EASY-nLC 1200 Preinstallation Requirements Guide Revision A

Thermo
EASY-nLC 1200
Preinstallation Requirements Guide
60053-97271 Revision A September 2015
© 2015 Thermo Fisher Scientific Inc. All rights reserved.
EASY-nLC, EASY-Spray, and Nanospray Flex are trademarks and Optima and Xcalibur are registered
trademarks of Thermo Fisher Scientific in the United States.
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registered trademarks of Advion Biosystems, Inc. Agilent and Varian are registered trademarks of Agilent
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Company. Teflon is a registered trademark of E.I. du Pont de Nemours and Company.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Revision History: Revision A September 2015
Hardware versions: EASY-nLC 1200 instrument
For Research Use Only. Not for use in diagnostic procedures.
IMPORTANT PREINSTALLATION INFORMATION... PLEASE READ
EASY-nLC Installation Request Form
Dear User:
Read the EASY-nLC 1200 Preinstallation Requirements Guide, and then print and complete the following
installation request form. After all items on the form are fulfilled, sign and date the form. Then, mail or fax this
form to your local Thermo Fisher Scientific sales/service office. The address and fax number for your local office
are located on the following pages.
 1. All laboratory remodeling has been completed.
 2. Your EASY-nLC instrument is on site.
 3. Principal operator will be available during the
installation/certification period.
 4. Sufficient bench space is available for all of the
equipment. List the following:
Width:
__________________________
Depth:
__________________________
Height:
__________________________
 5. Workbench can support four times the weight of
the instrument and is free from vibration.
EASY-nLC 1200
35kg (77.0 lb)
 6. Lighting is adequate.
 7. Main power is installed and is in compliance with
local electrical codes.
 8. Power for test and cleaning equipment is
installed.
 9. Power outlets are of the correct configuration.
Note NEMA type: ____________________
 10. Voltage of power outlet has been measured.
Note measured voltage: ______________
 11. Power is free from fluctuations due to slow
changes in the average voltage or changes due
to surges, sags, or transients.
 12. Air conditioning is adequate for temperature,
humidity, and particulate matter control. The
laboratory can be maintained at a constant
temperature, between 5 and 30 °C (41 and
86 °F).
 13. Relative humidity is between 20 and 80% with
no condensation.
 14. Instrument work area is free from magnetic
disruption and electrostatic discharge.
 15. LC/MS-grade water, acetonitrile, and formic acid,
are available for testing the performance of your
LC/MS system.
For best results, order the following solvent
blends from Thermo Fisher Scientific:
• LC/MS-grade 0.1% formic acid in water
• LC/MS-grade 80% acetonitrile, 20% water
with 0.1% formic acid
 16. Provision has been made for collecting the
solvent waste.
 17. One voice telephone line is installed near the
LC/MS system.
 18. One network connection is installed near
the instrument. The network firewall must allow
outgoing TCP/IP traffic from the EASY-nLC
instrument to the remote support server at
IP address 46.226.219.10, port 22.
 19. An appropriate MS detector with a nanospray ion
source is installed near the instrument.
 20. All relevant safety regulations are complied with.
Have any special acceptance specifications been agreed to in the contract?
If YES, attach full details of specifications.
Yes  No 
Is there any additional equipment that needs to be interfaced to the instrument? Yes  No 
If YES, attach full details of additional equipment.
Note: We reserve the right to invoice against the engineer’s time if the installation requirements are not met on the
date of the installation.
EASY-nLC Information Request Form (continued)
Print your name, company name, and company address clearly below:
Name _______________________________________________________
Company _____________________________________________________ Telephone____________________
Address ___________________________________________________________________________________
Address ___________________________________________________________________________________
City ______________________________________ State _____________ Country______________________
Signature _____________________________________________________ Date ________________________
Note After receiving this checklist, your local field service representative will contact you to schedule
installation.
Chromatography and Mass Spectrometry Offices
For up-to-date contact information, visit www.thermoscientific.com/wps/portal/ts/contactus.
North America
France
United States
(Also representing French-speaking North Africa,
Algeria, Morocco, and Tunisia)
1400 North Point Pkwy #10
West Palm Beach, FL 33407
E-mail:
[email protected]
Phone.........................[1] 800 532 4752
Fax .............................[1] 877 373 4006
Canada
2845 Argentia Road, Unit 4
Mississauga, Ontario, L5N 8G6
E-mail:
[email protected]
Phone.........................[1] 800 530 8447
Fax .............................[1] (905) 890 9161
Europe
Austria
Wehlistrasse 27b
A-1200 Wien
E-mail: [email protected]
Phone.........................[43] (0) 1 333 50 34-0
Fax .............................[43] (0) 1 333 50 34-26
Belgium
Clintonpark “Keppekouter”
Ninovesteenweg 198
B-9320 ERMEBODEGEM - AALST
E-mail: [email protected]
Phone.........................[32] (0) 2 482 3030
Fax .............................[32] (0) 2 482 3031
Denmark
Fruebjergvej 3
2100 København Ø
E-mail: [email protected]
Phone.........................[45] (70) 236267
Fax .............................[45] (70) 236263
16 Avenue du Québec
Silic 765
Z.A. de Courtaboeuf
F-91963 Les Ulis Cédex
E-mail: [email protected]
Phone .........................[33] (0) 1 60 92 49 50
Fax .............................[33] (0) 1 60 92 48 99
Germany
Im Steingrund 4-6
D-63303 Dreieich
E-mail: [email protected]
Phone .........................[49] (0) 6103 408 1050
Fax .............................[49] (0) 6103 408 1213
Italy
Strada Rivoltana
I-20090 Rodano (Milano)
E-mail: [email protected]
Phone .........................Numero Verde (800) 823 162
Fax .............................[39] (02) 95320 225
Netherlands
Takkebijsters 1
NL-4817 BL Breda
E-mail: [email protected]
Phone .........................[31] (0) 76 579 55 55
Fax .............................[31] (0) 76 581 09 61
Norway—see “Sweden, Norway, and Finland”
Spain
C/Valportillo I, no22 1a Planta
Edificio Caoba
ES-28108 Alcobendas - Madrid
E-mail: [email protected]
Phone .........................[34] (914) 845 965
Fax .............................[34] (914) 843 598
Finland—see “Sweden, Norway, and Finland”
Notes: The country code is enclosed in square brackets [ ]. The city code or area code is enclosed in parenthesis ( ).
Chromatography and Mass Spectrometry Offices—Continued
Europe—continued
Sweden, Norway, and Finland
Pyramidbacken 3
S-141 75 Kungens Kurva (Stockholm)
Sweden
E-mail: [email protected]
Phone.........................[46] (0) 8 556 468 20
Fax .............................[46] (0) 8 556 468 08
Switzerland
Neuhofstrasse 11
4153 Reinach
E-mail: [email protected]
Phone.........................[41] (617) 16 77 40
Fax .............................[41] (617) 16 77 20
United Kingdom
Stafford House
1 Boundary Park
Boundary Way
Hemel Hempstead
Hertfordshire HP2 7GE
E-mail: [email protected]
Phone ........................[44] (0) 870 241 1034
Fax .............................[44] (0) 1442 233 667
Australasia and Asia
Australia
P.O. Box 9092
5 Caribbean Drive
Scoresby, VIC 3179
E-mail: [email protected]
Phone.........................[61] 39757 4300
Fax .............................[61] 9763 1169
Japan
C-2F
3-9 Moriya-cho, Kanagawa-ku
Yokohama 221-0022
E-mail: [email protected]
Phone.........................[81] (45) 453 9100
Fax .............................[81] (45) 453 9110
P.R. China
7th Floor, 7F Tower West, Younghe Plaza
No. 28, Andingmen East Street
Beijing 100007
E-mail: [email protected]
Phone (free lines).......800 810 5118
...................................400 650 5118
Fax .............................[86] 10 88370548
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations. When the instrument is delivered to you, it meets all pertinent
electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name.
Changes that you make to your instrument might void compliance with one or more of these EMC and safety
standards. Changes to your instrument include replacing a part or adding components, options, or peripherals not
specifically authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and
safety standards, replacement parts and additional components, options, and peripherals must be ordered from
Thermo Fisher Scientific or one of its authorized representatives.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside
this manual pertaining to the safe use and operation of this product before using the device.
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific
instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one
person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: This instrument must be used in the manner specified by Thermo Fisher
Scientific to ensure protections provided by the instrument are not impaired. Deviations from specified instructions on
the proper use of the instrument include changes to the system and part replacement. Accordingly, order replacement
parts from Thermo Fisher Scientific or one of its authorized representatives.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument.
For manufacturing location, see the label on the instrument.
WEEE Compliance
This product complies with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the
European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway.
If this product is located in Europe and you want to participate in the Thermo Fisher Scientific Business-to-Business
(B2B) Recycling Program, send an email request to [email protected] with the following information:
• WEEE product class
• Name of the manufacturer or distributor (where you purchased the product)
• Number of product pieces, and the estimated total weight and volume
• Pick-up address and contact person (include contact information)
• Appropriate pick-up time
• Declaration of decontamination, stating that all hazardous fluids or material have been removed from the product
For additional information about the Restriction on Hazardous Substances (RoHS) Directive for the European Union,
search for RoHS on the Thermo Fisher Scientific European language websites.
IMPORTANT This recycling program is not for biological hazard products or for products that have been medically
contaminated. You must treat these types of products as biohazard waste and dispose of them in accordance with
your local regulations.
Conformité DEEE
Ce produit est conforme avec la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et
Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs sociétés de recyclage dans chaque état membre de l’Union
Européenne et ce produit devrait être collecté ou recyclé par celle(s)-ci. Pour davantage d'informations, rendez-vous sur
la page www.thermoscientific.fr/rohs.
WEEE Konformität
Dieses Produkt entspricht der EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC. Es ist
mit dem folgenden Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten
getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Weitere Informationen
finden Sie unter www.thermoscientific.de/rohs.
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Cautions and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Thermo Scientific
Chapter 1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Meeting the Site Requirements for the EASY-nLC Instrument . . . . . . . . . . . . . . 1
Laboratory Requirements for the EASY-nLC Instrument . . . . . . . . . . . . . . . . . . 2
Chapter 2
Site Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Telephone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Internet Access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Chapter 3
Operating Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Temperature and Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Air Conditioning Load . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Vibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Lighting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Particulate Matter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Electrostatic Discharge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Chapter 4
Line Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Quality of Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Power Monitoring Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Power Conditioning Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Electrical Outlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Line Voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Grounding Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Maximum Load Capacity for Each Fourplex Outlet . . . . . . . . . . . . . . . . . . . 18
Power Supply Cords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Uninterruptible Power Supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Technical Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
EASY-nLC 1200 Preinstallation Requirements Guide
xi
Contents
Chapter 5
Waste and Ventilation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Chapter 6
Solvents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Chapter 7
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Common Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Additional Installation Peripherals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Chapter 8
Instrument Arrival . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Receiving Shipping Packages and Reporting Damage . . . . . . . . . . . . . . . . . . . . 27
Shipment Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Domestic Shipments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
International Shipments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
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EASY-nLC 1200 Preinstallation Requirements Guide
Thermo Scientific
Preface
Preface
This guide describes what you as the customer must do to prepare for the installation of the
Thermo Scientific EASY-nLC™ (nanoflow liquid chromatography) instrument. Prior to
delivery of the instrument and before making an installation appointment with a Thermo
Fisher Scientific field service engineer, use this guide in planning and preparing your
laboratory site. Read each topic carefully to ensure that your laboratory is ready for the
instrument installation.
Contents
• Related Documentation
• Cautions and Special Notices
• Contacting Us
 To suggest changes to documentation or to Help
Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.
Thank you in advance for your help.
Related Documentation
In addition to this guide, Thermo Fisher Scientific provides the following documents for the
EASY-nLC instrument as PDF files:
• EASY-nLC 1200 User Guide for the Xcalibur Data System
• EASY-nLC 1200 Getting Started Guide (for the touch-screen software)
• EASY-nLC 1200 Troubleshooting and Maintenance Guide
• Safety and Regulatory Guide
The EASY-nLC 1200 also ships with a printed copy of the Safety and Regulatory Guide.
This guide contains important safety information about Thermo Scientific™ liquid
chromatography (LC) and mass spectrometry (MS) systems. Make sure that all lab
personnel have read and have access to this document.
You can find the EASY-nLC Series manuals in the following locations:
• On the USB flash drive provided in the EASY-nLC accessory kit
• On the Thermo Scientific Customer Manuals Web Site
• On the data system computer
Thermo Scientific
EASY-nLC 1200 Preinstallation Requirements Guide
xiii
Preface
 To view the product manuals on the data system computer
Choose Start > All Programs > Thermo Instruments > Manuals > LC Devices >
Thermo.
 To view the EASY-nLC Help
• From the EASY-nLC view of the Instrument Setup window, choose Help >
Thermo EASY-nLC Help to open the Help to the Welcome topic.
• To view the Help topic for the current view, page, or dialog box, press the F1 key for
information about setting parameters. Or, from the EASY-nLC view of the
Instrument Setup window, choose Help > Help On Current Item.
For more information, visit www.thermoscientific.com.
Cautions and Special Notices
Make sure to observe the safety and special notices that appear in boxes.
The following two stickers appear on the EASY-nLC instrument:
• The sticker below warns you that the instrument includes a sharp needle and moving
parts that are accessible to the operator. To prevent personal injury or damage to parts of
the EASY-nLC instrument, take care when loading samples into the instrument’s tray
compartment.
WARNING:
AVERTISSEMENT:
SHARP NEEDLE AND MOVING PARTS.
KEEP HANDS CLEAR.
AIGUILLE POINTURE ET PARTIES MOUVANTES.
TENIR LES MAINS A` L’ECART
´
• The sticker below alerts you to consult this manual for instructions on how to operate the
instrument.
The safety and special notices in this manual include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
CAUTION Highlights lifting hazards.
xiv
EASY-nLC 1200 Preinstallation Requirements Guide
Thermo Scientific
Preface
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or may contain information that is critical for optimal
performance of the instrument.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
IMPORTANT General Residual Hazards
WARNING—Biohazard Human-sourced material, for example microorganisms, cell
cultures, tissues, body fluids, and other biological agents can transmit infectious diseases.
To avoid infections with these agents:
• Assume that all biological substances are at least potentially infectious.
• Wear personal protective equipment as required by the hazard and follow good
laboratory practice.
• Dispose of bio-hazardous waste in an environmentally safe manner that is consistent
with local regulations. Follow a regulated, approved waste disposal program.
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
 To contact Technical Support
Phone
(U.S. and Canada) 800-532-4752
Web site
For up-to-date contact information for other locations, go to
www.thermoscientific.com/wps/portal/ts/contactus.
E-mail
(North and South America)
[email protected]
(Other continents)
[email protected]
 (U.S. and Canada) To contact Customer Service for ordering information
Thermo Scientific
Phone
800-532-4752
Fax
561-688-8731
E-mail
[email protected]
Web site
www.thermoscientific.com
EASY-nLC 1200 Preinstallation Requirements Guide
xv
Preface
For all other sales and service contacts for Thermo Scientific CMD products, see
“Chromatography and Mass Spectrometry Offices” on page v.
 To order consumable and spare parts for the EASY-nLC instrument
For the EASY-nLC 1200 instrument, go to
www.thermoscientific.com/nanoLCMS
 To copy manuals from the Internet
Go to mssupport.thermo.com and click Customer Manuals in the left margin of the
window.
 To suggest changes to documentation or to Help
• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.
• Send an e-mail message to the Technical Publications Editor at
[email protected]
xvi
EASY-nLC 1200 Preinstallation Requirements Guide
Thermo Scientific
1
Introduction
This document describes the requirements for the laboratory where you plan to install the
EASY-nLC instrument. If you have any questions prior to the installation appointment,
contact your local sales representative or our remote support center:
• For North and South America, contact your local sales representative at
[email protected]
• For all other continents, contact your local sales representative at
[email protected]
Contents
• Meeting the Site Requirements for the EASY-nLC Instrument
• Laboratory Requirements for the EASY-nLC Instrument
Meeting the Site Requirements for the EASY-nLC Instrument
To confirm that you have met the site requirements in this document, fill out and forward a
signed copy of the Installation Request Form at the beginning of this document.
The EASY-nLC instrument operates reliably under carefully controlled environmental
conditions. As the purchaser, you are responsible for providing a suitable location, a suitable
operating environment, a source of power of acceptable quality, correct solvent supplies, and
proper waste systems.
Operating an EASY-nLC instrument or maintaining it in a condition outside the operating
environment specifications described in this guide might cause failures of many types. The
repair of such failures is specifically excluded from the standard warranty and service contract
coverage.
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EASY-nLC 1200 Preinstallation Requirements Guide
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1
Introduction
Laboratory Requirements for the EASY-nLC Instrument
Laboratory Requirements for the EASY-nLC Instrument
Table 1 summarizes the installation requirements for the EASY-nLC 1200 instrument.
Table 1. Laboratory requirements
Specification
EASY-nLC 1200
Instrument dimensions
Width
36 cm (14.2 in.)
Deptha
38 cm (15.1 in.)
Heightb
45 cm (17.7 in.)
Weightc
35 kg (77.2 lb)
Solvents
Use only LC/MS-grade solvents with the EASY-nLC instrument. For more information, see
Chapter 6, “Solvents.”
Solvent A
0.1% formic acid in water
Solvent B
0.1% formic acid in max 95% acetonitrile in water.
Wash bottle 3
0.1% formic acid in water
Power requirements
For UPS dimensioning, assume 250 W.
120 Vac
±10%; 50/60 Hz; 250 VA
230 Vac
±10%; 50/60 Hz; 250 VA
Note The EASY-nLC instrument and the Thermo Scientific mass spectrometer require
one outlet each. The nanospray ion source requires three outlets. The optional data system
computer, Ethernet switch, monitor, and printer also require one outlet each. For more
information, see Chapter 4, “Line Power.”
Laboratory environment
For optimal instrument performance, ensure the following:
• The benchtop must be vibration free and well lit.
• The air conditioning system must be capable of maintaining a temperature range of 5
to 30 °C with minimum temperature fluctuations and a relative humidity between 20
to 80% with no condensation.
• The laboratory air must be free from excessive dust, smoke, or other particulate matter
in excess of 5 μm—that is, fewer than 3500000 particles per cubic meter
(100000 particles per cubic foot).
2
a
Provide additional space behind the instrument for ventilation and access to the back panel connections.
b
Provide additional space above the instrument for the solvent bottles.
c
Ensure that the laboratory benchtop can support four times the system weight.
EASY-nLC 1200 Preinstallation Requirements Guide
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2
Site Preparation
Before a Thermo Fisher Scientific field service engineer can install the EASY-nLC instrument,
you must prepare the site. Supporting the weight of the EASY-nLC instrument, (optional)
LC detector, and mass spectrometer requires large and strong workbenches. Each LC/MS
system also has specific power requirements. You must install a telephone line and an Internet
connection within reach of the workbench.
You are responsible for providing an acceptable installation site.
Contents
• Shipping
• Placement
• Telephone
• Internet Access
Shipping
The EASY-nLC instrument and all auxiliaries ship in a box, which is then placed on a pallet.
Table 2 lists the dimensions of the box and the complete shipping consignment on the pallet.
Table 2. Shipping dimensions
Dimension
Box with protective padding
Pallet with shipping consignment
Width
60 cm (23.6 in.)
80 cm (31.5 in.)
Length
55 cm (21.7 in.)
60 cm (23.6 in.)
Height
65 cm (25.6 in.)
80 cm (31.5 in.)
The total weight of the shipping container is 45 kg (99 lb).
Thermo Scientific
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2
Site Preparation
Placement
Because the box comes with special protective padding, save the box for possible reuse.
CAUTION If the instrument shipping container, Shock Watch, or other indicators show
any evidence of damage or mishandling during shipment, do NOT open the container.
Call your Thermo Fisher Scientific representative for instructions on what to do. If there
is no evidence of shipping damage or mishandling, then you can proceed with the
instructions that follow.
Placement
Out of the box, the instrument weighs approximately 35 kg (77 lb).
CAUTION The EASY-nLC instrument is too heavy for one person alone to lift. To avoid
personal injury, use two people to move the instrument to a table cart for transport.
Table 2 lists the dimensions of the EASY-nLC 1200 instrument.
Table 3. EASY-nLC instrument dimensions
Dimension
EASY-nLC 1200
Width
36 cm (14.2 in.)
Depth
38 cm (15.1 in.)
Height
45 cm (17.7 in.)
Provide at least 15 cm (6 in.) of free space at the back of the instrument to ensure proper air
circulation and a least 15 cm (6 in.) above the instrument for the solvent bottles. Place the
EASY-nLC instrument as close as possible to the MS detector (mass spectrometer) to
minimize dead volume in the transfer lines.
Figure 1 shows the EASY-nLC instrument installed as part of an LC/MS system with a data
system computer, an Ethernet switch, a Thermo Scientific mass spectrometer, and an
EASY-Spray™ ion source.
Note The EASY-Spray source has a power supply unit that connects to an electrical wall
outlet. The NanoFlex ion source gets its power from the mass spectrometer.
The Thermo Scientific MS detector and the EASY-nLC instrument communicate with the
data system computer through an Ethernet switch that is connected to the data system
computer. Connecting the data system computer to your local area network requires an
additional network card.
IMPORTANT For remote diagnostics, connect the EASY-nLC instrument directly to the
Internet access port using the supplied Category 6 network cable.
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2
Site Preparation
Placement
A contact closure cable between the EASY-nLC instrument and the Thermo Scientific
MS detector synchronizes the run timing.
Table 5 on page 16 describes the line power connections.
Note With the 3.2 or later version of the EASY-nLC touch-screen application, the
EASY-nLC instrument provides line power to the EASY-Spray ion source by way of a
P-bus cable. For information about connecting the EASY-Spray ion source to the
EASY-nLC instrument, refer to the EASY-nLC Series Getting Started Guide (touch-screen
application version 3.2).
Figure 1.
LC/MS system setup with a data system computer connected to the local area network
Ion source monitor
LTQ Series
MS detector
EASY-nLC
instrument
Data system
computer
Load
Detector
Inject
Waste
Power
Vacuum
Communication
System
Scanning
EASY - nLC 1200
EASY - nLC II
Ethernet
switch
Pump On
Power line
connection
for the EASY-nLC
instrument
Ethernet cable from
the Ethernet switch
to the data system computer
Intranet connection to the data system computer
(requires an additional network card)
Thermo Scientific
Ethernet cable from
the EASY-nLC instrument
to the Ethernet switch
Power line
connections for the
EASY-Spray monitor
and camera
Power line
connection
for the
MS detector
Ethernet cable from
the MS detector to
the Ethernet switch
Contact closure cable
from the
EASY-nLC instrument
to the MS detector
EASY-nLC 1200 Preinstallation Requirements Guide
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2
Site Preparation
Telephone
Telephone
Install a telephone in your laboratory near the instrument so that, if necessary, you can
conveniently operate the instrument while in contact by telephone with Thermo Fisher
Scientific Technical Support. Place the voice telephone outlet within 2 m (6 ft) of your
LC/MS system.
IMPORTANT Your instrument is designed to work in a controlled electromagnetic
environment. Do not use radio frequency transmitters, such as mobile phones, in close
proximity to the instrument.
Internet Access
For online support and monitoring, provide Internet access for the EASY-nLC instrument
within reach of the laboratory benchtop, and ask your IT system administrator to set up the
network firewalls to allow outgoing traffic to IP address 46.226.219.10, port 22.
IMPORTANT To connect to the Thermo Fisher Scientific support server, which is a
Linux™ server, the EASY-nLC instrument uses the Secure Shell (SSH™) network protocol
for secure data communication.
When you request a connection to the support server by using the EASY-nLC
instrument’s touch-screen controls, the SSH program on the EASY-nLC instrument opens
a connection to the support server. This connection includes an SSH tunnel through
which the remote support software and a Thermo Fisher Scientific technical support
engineer can connect back to the EASY-nLC instrument to perform various operations on
the EASY-nLC instrument.
To use the remote support feature, the firewalls for your local network must allow
outgoing TCP/IP traffic from the EASY-nLC instrument to the support server at
IP address 46.226.219.10, port 22.
When installing the EASY-nLC instrument, your local Thermo Fisher Scientific field service
engineer sets up the instrument to use dynamic IP addresses through a local DHCP server, or
gives it a fixed IP address (probably through your IT system administrator).
Figure 2 shows the EASY-nLC instrument connected to a laboratory LAN port with Internet
capability by way of a shielded Ethernet cable.
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EASY-nLC 1200 Preinstallation Requirements Guide
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2
Figure 2.
Site Preparation
Internet Access
Laboratory LAN port connection
Shielded Ethernet cable
LAN port on the
back panel of the
EASY-nLC
instrument
P-BUS
IN3 OUT3 IN2 OUT2 IN1 OUT1
USB
RS-232
LAN
MONITOR
HARD
DRIVE
I
O
110-120V▼
LAN MAC ADDRESS: 00-6--E0-45-4E-28
Laboratory
LAN port
with Internet
capability
220-240▼
S/N
E A S Y- n L C 1 0 0 0
P/N
Made in U.S.A.
120/230 V : 50/60 Hz: 250 W
Fuse at 120 V– : T5 AL 250 V
Fuse at 230 V– : T2.5 AL 250 V
LC-011xxx
LC120
This device complies with Part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and
(2) This device must accept any interference received, including interference
that may cause undesired operation.
This instrument is for research use only.
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3
Operating Environment
Attention to the operating environment will ensure continued high performance of your
EASY-nLC instrument. Any expenditures for air conditioning are more than offset by good
sample throughput and reduced repair costs.
You are responsible for providing the operating environment necessary for proper operation of
the EASY-nLC instrument.
Contents
• Temperature and Humidity
• Air Conditioning Load
• Vibration
• Lighting
• Particulate Matter
• Electrostatic Discharge
Temperature and Humidity
The air conditioning system must be capable of maintaining a temperature range of
5 to 30 °C (41 to 86 °F) with minimum temperature fluctuations. Temperature fluctuations
greater than ±5 °C (±9 °F) can adversely affect chromatographic performance of the LC
instrument.
IMPORTANT As the laboratory temperature increases, instrument reliability decreases. All
electronic components generate heat while operating. For the components to continue to
operate reliably, ensure that this heat is dissipated to the surrounding air.
There must be a good flow of room air around the instrument, and the air conditioning
system must be capable of maintaining a constant temperature in the immediate vicinity of
the instrument.
IMPORTANT To maintain instrument performance, do not place the EASY-nLC
instrument under an air duct, near windows, or near heating or cooling sources.
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3
Operating Environment
Air Conditioning Load
Maintain the relative humidity of the operating environment between 20 to 80% with no
condensation.
Operating an EASY-nLC instrument in an environment with very low humidity can cause
static electricity to accumulate and discharge, which can shorten the life of the electronic
components. Operating the instrument in an environment with high humidity can cause
condensation, oxidation, and short circuits. It can also cause dust to accumulate, which can
block filters on cooling fans.
To ensure that your laboratory is always within the required temperature and humidity
specifications, Thermo Fisher Scientific recommends that you equip your laboratory with a
temperature/humidity monitor.
Air Conditioning Load
The typical air conditioning load for an EASY-nLC instrument is approximately 125 W.
Vibration
Keep floors free of vibration caused, for example, by nearby equipment.
Lighting
For comfort and safety in performing LC instrument operations, make sure that your
laboratory provides excellent lighting.
Particulate Matter
Ensure that the air in your laboratory is free from excessive dust, smoke, or other
particulate matter in excess of 5 μm—that is, fewer than 3 500 000 particles per cubic meter
(100 000 particles per cubic foot).
Dust can clog the air filters, which causes reduced air flow around electronic components.
Dust on electronic components can act as an insulating blanket, which reduces the transfer of
heat from the components to the surrounding air.
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3
Operating Environment
Electrostatic Discharge
Electrostatic Discharge
Electrostatic discharge (ESD) can damage the electronic components of your LC/MS system.
The discharge of static electricity is not perceptible to humans until the potential is at least
4000 V. However, a discharge of electrostatic potential as small as 50 V can damage many
electronic components. While ESD damage can be catastrophic and cause your system to
cease functioning, more commonly, ESD damage might cause latent problems that are
detrimental to sensitive electrical components, causing premature failures.
The LC/MS system is designed to withstand electrostatic discharges up to 4 kV (air discharge)
and 4 kV (contact discharge) with all panels in place. However, removing the panels and
handling the printed circuit boards (PCBs) without proper precautions might damage the
electrical components or cause them to fail prematurely.
Static electricity can develop in a variety of ways. Some examples follow:
• Walking across a carpet in a room that is at 20 percent relative humidity can generate as
much as 35000 V of electrostatic potential on the surface of your body. A similar trip in a
room at 80 percent relative humidity generates about 1500 V of electrostatic potential.
• Sitting and working in a chair padded with polyurethane foam in a room at 20 percent
relative humidity can cause as much as 18000 V of electrostatic potential to develop on
your skin. At 80 percent relative humidity, the electrostatic potential can be as much as
1500 V.
• Working in a laboratory coat and clothing made of synthetic fibers can cause static
electricity to accumulate on your skin.
• Using Styrofoam™ cups and packing materials results in a considerable electrostatic
charge.
Because of ESD, Thermo Fisher Scientific recommends the following precautions, especially
when operating your LC/MS system at the lower end of the relative humidity specification:
• Use a static-dissipating floor covering (such as tile or conductive linoleum) in the room
that houses your instrument.
• Use laboratory chairs covered with natural fibers or other static-dissipating material.
• Wear a laboratory coat and clothing made of natural fiber or other static-dissipating
material when you are operating the instrument.
• Keep Styrofoam cups or packing materials away from the instrument.
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4
Line Power
The quality of line power delivered to your EASY-nLC instrument can affect its performance
and longevity. To ensure that your LC instrument performs optimally and is not damaged by
line power fluctuations, verify that your laboratory electrical supply complies with all power
quality requirements.
You are responsible for providing a power source of acceptable quality to operate your
LC instrument.
Contents
• Quality of Power
• Power Monitoring Devices
• Power Conditioning Devices
• Electrical Outlets
• Power Supply Cords
• Uninterruptible Power Supply
• Technical Assistance
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4
Line Power
Quality of Power
Quality of Power
The quality of power supplied to your EASY-nLC instrument is very important. The line
voltage must be stable and within the specifications listed in this guide. The line voltage must
be free of fluctuations due to slow changes in the average voltage, surges, sags, or transients.
Table 4 contains definitions for the three most common voltage disturbances.
Table 4. Common voltage disturbances
Voltage disturbance
Definition
Slow average
A gradual, long-term change in average root mean square
(RMS) voltage level, with typical durations greater than
2 seconds
Sags and surges
Sudden changes in average RMS voltage level, with typical
durations between 50 microseconds and 2 seconds
Transients or impulses
Brief voltage excursions of up to several thousand volts with
durations up to 50 microseconds
CAUTION Constant high line voltage, impulses, or surges in voltage can cause overheating
and component failures. Constant low line voltage or sags in voltage can cause the system
to function erratically or not at all. Transients, even a few microseconds in duration, can
cause electronic devices to fail catastrophically or to degrade and eventually shorten the
lifetime of your system. For these reasons, make sure that you establish the quality of the
line voltage in your laboratory before installing an EASY-nLC instrument.
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4 Line Power
Power Monitoring Devices
Power Monitoring Devices
Monitor the quality of your line power with a power line disturbance analyzer.
This type of device provides a continuous record of line performance by analyzing and
printing out information on three types of voltage disturbances: slow average, sag and surge,
and transient. The Dranetz™ power line disturbance analyzer is a device capable of detecting
and recording most types of line power problems.1 You can rent power line analyzers from
electrical equipment suppliers.
Monitor the power line 24 hours a day, for seven consecutive days. If inspection of the
printout indicates disturbances, take corrective action.
Power Conditioning Devices
You can correct a line voltage problem using various line voltage conditioning devices. If you
have good regulation but the power line disturbance analyzer shows transient voltages, then an
isolation/noise-suppression transformer can resolve the problem. If there are both transient
and regulation problems, consider power conditioners that can control these problems.
When the line voltage is free from voltage sags, surges, and impulses but is more than
10 percent outside of the voltage specifications, a buck/boost transformer can lower
(buck 10 percent) or raise (boost 10 percent) the line voltage.
Each buck/boost transformer is encased in a metal housing approximately 13 × 13 × 26 cm
(5 × 5 × 10 in.) and is equipped with a 2 m (6 ft) power cable. To order the buck/boost
transformer kit (P/N OPTON-01460), contact Thermo Fisher Scientific San Jose, and then
have your electrician install the buck/boost transformer before a Thermo Fisher Scientific
field service engineer installs your LC system. The transformer kit includes the installation
instructions.
CAUTION For compliance and safety, recognized domestic and international
organizations (for example, UL, CSA, TÜV, and VDE) must certify your uninterruptible
power supply (UPS) devices.
1
Thermo Scientific
Thermo Fisher Scientific does not endorse any power monitoring company, nor does it endorse products other
than its own. Companies and products listed in this guide are given as examples only.
EASY-nLC 1200 Preinstallation Requirements Guide
15
4
Line Power
Electrical Outlets
Electrical Outlets
Before you install an EASY-nLC instrument, plan your power system. See Table 5 for an
example of the number of power outlets that your laboratory might require. A stand-alone
EASY-nLC instrument requires one outlet. A Thermo Scientific MS detector and its
associated data system hardware require two fourplex outlets. The nanospray ion source
requires three power outlets.
Note For information about the power requirements for a Thermo Scientific mass
spectrometer, refer to its preinstallation requirements guide or getting connected guide.
Locate the electrical outlets within reach of the workbench where you plan to install the
LC/MS system. For information about the power cords supplied with the EASY-nLC system,
see “Power Supply Cords” on page 18.
Table 5. Laboratory power outlets for an LC/MS system with an EASY-nLC instrument
Item
Outlets
EASY-nLC instrument
1
Data system for the MS detector and optional control of the EASY-nLC
instrument
• CPU
1
• Monitor
1
• Printer
1
• Ethernet switch
1
MS detector and ion source
• Mass spectrometer
1
• Nanospray ion source
2 or 3
(Optional) High-intensity lamp: For help in instrument maintenance.
1
(Optional) Laboratory stereoscope: For inspecting fused-silica parts, used when
performing nanoflow or microfluidic experiments.
1
Total outlets required for this configuration
9 to 11
Note Additional power outlets might be required for test and cleaning equipment, such as
an ultrasonic bath. Thermo Fisher Scientific recommends that there be several additional
power outlets close to the workbench space within your laboratory.
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4 Line Power
Electrical Outlets
These topics describe the line voltage, maximum load capacity, and grounding requirements
for the electrical outlets:
• Line Voltage
• Grounding Requirements
• Maximum Load Capacity for Each Fourplex Outlet
Line Voltage
The EASY-nLC system is equipped with an auto-ranging power entry module and can
operate within the range of 120/230 Vac ±10% 50/60 Hz; 250 VA.
Grounding Requirements
Installing an EASY-nLC instrument requires a minimum of one outlet. The electrical outlet
must be earth ground hard-wired to the main panel.
If you are using the EASY-nLC instrument as an inlet to an MS detector, the LC/MS system
might require two or more fourplex outlets. The interconnected electrical outlets for the
LC instrument, the MS detector, and the data system computer must have a common point
to one ground connector.
CAUTION When using the EASY-nLC instrument as an inlet to an LC/MS system, the
LC instrument, the MS detector, and the (optional) data system hardware must have a
common ground.
Connecting the hardware to external grounds at different potentials can do the following:
• Create a ground loop that causes noise and interference.
• Damage the EASY-nLC instrument’s built-in computer.
CAUTION Improper grounding of the EASY-nLC instrument creates an electrical safety
hazard.
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4
Line Power
Power Supply Cords
Maximum Load Capacity for Each Fourplex Outlet
Do not exceed the electrical outlet specifications. The maximum load for a 115 Vac fourplex
outlet is typically 20 A, and the maximum load for a 230 Vac fourplex outlet is typically 16 A.
The maximum current drawn by the EASY-nLC instrument depends on the line voltage:
• For 115 Vac line power, the EASY-nLC instrument draws up to 5.0 A.
• For 230 Vac line power, the EASY-nLC instrument draws up to 2.5 A.
CAUTION Never connect an MS detector and an EASY-nLC instrument to the same
electrical outlet circuit. Connect the EASY-nLC instrument and the MS detector to
separate electrical outlets.
Power Supply Cords
Each EASY-nLC instrument comes with a 2.5 m (8.2 ft) long power cord.
The data system hardware components that come with each Thermo Scientific MS detector
are approximately 1.8 m (6 ft) long.
Uninterruptible Power Supply
If your local area is susceptible to corrupted power or power disruptions, install an
uninterruptible power supply (UPS) in your laboratory.
CAUTION For compliance and safety, your uninterruptible power supply (UPS) devices
must be certified by recognized domestic and international organizations (for example,
UL, CSA, TÜV, and VDE).
Technical Assistance
Occasionally, you might encounter line power sources of unacceptable quality that adversely
affect the operation of the EASY-nLC instrument. Correcting line power problems is your
responsibility. Contact your local office for Thermo Scientific products for assistance in
monitoring the line voltage in your laboratory and in selecting a line conditioner.
Specifying power conditioning equipment is a complex task that is best handled by a company
or consultant specializing in that field. Contact your local Thermo Fisher Scientific office for
assistance in locating a power consultant in your area.
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5
Waste and Ventilation
The waste and exhaust arrangements for your EASY-nLC instrument can affect the proper
performance of the instrument. You must collect and dispose of solvent wastes properly.
You are responsible for providing the proper waste disposal and ventilation systems required to
operate your LC/MS system.
Waste solvent from the needle wash and purge steps collects in the waste bottle within the
EASY-nLC instrument. The solvent from the pre-column equilibration step routes to the
waste beaker within the EASY-nLC instrument. The maximum flow rate for a purge cycle is
300 μL/min.
In addition to providing a proper waste disposal system, you must also ensure that your
laboratory is adequately ventilated to prevent the buildup of solvent fumes.
Thermo Scientific
EASY-nLC 1200 Preinstallation Requirements Guide
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6
Solvents
You are responsible for providing the high-purity solvents required to install and operate the
EASY-nLC instrument.
The installation solvents for the EASY-nLC instrument are as follows:
Solvent A:
Solvent B:
Wash solvent 3:
LC/MS-grade 0.1% formic acid in water
0.1% formic acid in a mixture of 80% acetonitrile in water.
LC/MS-grade 0.1% formic acid in water
CAUTION Solvent contaminants can cause system blockages and poor spray stability.
To minimize instrument problems caused by solvent contaminants, follow these
guidelines for the EASY-nLC instrument:
• Use only LC/MS-grade solvents.
• Use only commercially manufactured LC/MS-grade solvent blends, as mixing
solvents in a typical laboratory environment can introduce contaminants as well as
solvent concentration errors.
• Do not filter LC/MS-grade solvents, as filtering solvents can introduce contaminants.
• Do not use HPLC-grade solvents, as HPLC-grade solvents contain more
contaminants than LC/MS-grade solvents.
• Do not use water from laboratory purification systems, as laboratory purified water
contains more contaminants than LC/MS-grade water.
IMPORTANT For best results, use the Optima™ brand of LC/MS-grade solvents and
solvent blends supplied by Thermo Fisher Scientific. These solvents are manufactured in
facilities with an ISO 9001:2008 certified quality system to ensure optimum quality and
product uniformity.
To prepare these solutions, order the appropriate LC/MS-grade solvents and solvent blends.
Table 6 lists the solvents that you can order from Thermo Fisher Scientific.
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6
Solvents
Table 6. Solvents
Solvent/reagent
Grade
Water
Optima™ UHPLC-MS 1 L
A956-1
Acetonitrile
Optima™ UHPLC-MS 1 L
A458-1
Formic acid (99.5%)
Optima™ LC/MS
10×1mL
A117-10X1AMP
Water with 0.1% formic acid
Optima™ LC/MS
1L
LS118-1
80% acetonitrile, 20% water with Optima™ LC/MS
0.1% formic acid
500mL
LS122-500
0.1% formic acid in acetonitrile
1L
LS120-1
Optima™ LC/MS
Size
Catalog number
CAUTION Store and handle all chemicals in accordance with standard safety procedures.
The Optima LC/MS mobile phases can be ordered through www.fishersci.com (search for
Optima LC/MS)
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7
Installation
Before installation, make sure that all preparations described in the previous chapters are
complete.
When you have completed your laboratory site preparation, the EASY-nLC Installation
Request Form has been mailed or faxed to your local office for Thermo Scientific products,
and the instrument has been delivered, call your local Thermo Fisher Scientific office to
arrange for an installation date.
Contents
• Spare Parts
• Common Replacement Parts
• Additional Installation Peripherals
• Training
• Preventive Maintenance
Spare Parts
Table 7 lists the spare parts supplied with the EASY-nLC 1200 instrument.
Note The EASY-nLC instrument comes with a contact closure cable for a Thermo
Scientific MS detector. If you plan to connect the nanoflow LC instrument to a
non-Thermo Scientific MS detector, order the appropriate contact closure cable
(see Table 9 on page 25).
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7
Installation
Common Replacement Parts
Table 7. Spare parts shipped with the EASY-nLC 1200 instrument
Description
Part number
Autosampler injection needle
LC302
Autosampler vials with caps (24)
N/A
Adapter, 8 × 12 microtiter plate
LC191
Cable, contact closure, Thermo
Scientific MS detector
LC160
Cables, Ethernet (2)
N/A
Cord, Power
N/A
Fuses
110 V, 5 A
LC157
230 V, 2.5 A
LC158
Microtiter plates, 8 × 12 (2) and
silicone mats (2)
N/A
Seals, piston (4) and installation tool
LC510
Solvent bottles
LC186
Tubing, column out
LC560
Wash bottles (6)
LC182
Wash insert (1)
LC184
Union, leak test, stainless steel
(Viper union)
SC900
Common Replacement Parts
For proper maintenance of your EASY-nLC instrument, Thermo Fisher Scientific
recommends that you maintain an inventory of replacement parts.
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7 Installation
Additional Installation Peripherals
Table 8 lists the annual consumption of common replacements parts for the EASY-nLC 1200
instrument.
Table 8. Common replacement parts for the EASY-nLC 1200 instrument
Description
Part
number
Annual
consumption
Pump Piston Seal Replacement Kit
(contains four piston seals and the piston seal tool)
LC510
4 piston seals
(1/pump)
Column Out solvent line
LC560
2
Waste In solvent line
LC562
1
Autosampler needle, ASC model
LC302
2
Replacement filter discs
(contains two 10 μm filter discs)
LC232
2 filter discs
(1/solvent bottle)
Additional Installation Peripherals
To install the EASY-nLC instrument as an inlet to an MS detector, do the following:
• Order the appropriate ion source.
Thermo Fisher Scientific recommends that you use the Thermo Scientific Nanospray
Flex ion source.
• Ensure that the mass spectrometer and ion source are on site before the scheduled
installation date.
• If necessary, order a contact closure cable compatible with your MS detector.
Table 9 lists the contact closure cables that Thermo Fisher Scientific supplies for the
EASY-nLC instrument.
Table 9. Contact closure cables
MS detector
Thermo Scientific
Part number
Thermo Scientific (supplied with the EASY-nLC instrument)
LC160
ABI/MDS/Sciex™
LC161
Bruker™/Agilent™
LC162
Waters™/Micromass™
LC163
Varian™
LC164
EASY-nLC 1200 Preinstallation Requirements Guide
25
7
Installation
Training
Training
When your new EASY-nLC instrument is on site and ready for installation, a Thermo Fisher
Scientific field service engineer will install it.
During the installation, the field service engineer demonstrates the basics of equipment
operation and routine maintenance. You are responsible for ensuring that the appropriate
personnel are present for this training session.
Do not plan to use your new instrument for sample analysis until the installation is complete
and you have signed the acceptance form.
Preventive Maintenance
Routine and preventive maintenance of the EASY-nLC instrument is your responsibility.
Regular preventive maintenance is essential. It increases the life of the instrument,
maximizes the uptime of your instrument, and provides you with optimum instrument
performance. For more information on maintenance procedures, refer to the user guide for
the EASY-nLC instrument.
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EASY-nLC 1200 Preinstallation Requirements Guide
Thermo Scientific
8 Instrument Arrival
Receiving Shipping Packages and Reporting Damage
Instrument Arrival
Electronic equipment carriers that specialize in the handling of delicate machinery ship the
EASY-nLC instrument to your site. Occasionally, however, equipment is damaged in transit.
You are responsible for checking the shipping packages upon arrival.
Contents
• Receiving Shipping Packages and Reporting Damage
• Shipment Methods
Receiving Shipping Packages and Reporting Damage
When your instrument arrives, move it to a protected location indoors. If you have questions
about moving your instrument, contact your local office for Thermo Fisher Scientific
products.
If the instrument shipping container, Shock Watch, or other indicators show evidence of
damage or mishandling during shipment, do NOT open the container. Call your Thermo
Fisher Scientific representative for further instructions.
The preface of this guide and the contact information following the Installation Request Form
list telephone and fax numbers for service offices.
IMPORTANT Freight insurance requires that you note obvious damage on the receiving
documents. Thermo Fisher Scientific does not accept liability for damage if materials are
received with obvious damage AND the damage is not recorded on the receiving
documents.
Take the following precautions when receiving material:
• Check carefully for obvious damage or evidence of rough handling.
• Note any apparent external damage on all copies of the receiving documents and describe
briefly the extent of the damage. Have the driver sign (or initial) next to your comments
to signify agreement with your observations.
• To report the damage, contact your local service office.
Thermo Scientific
EASY-nLC 1200 Preinstallation Requirements Guide
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8
Instrument Arrival
Shipment Methods
Shipment Methods
If the instrument is damaged in transit, the shipment method determines the party who
assumes the risk of damage and files a claim against the carrier—Thermo Fisher Scientific or
the purchaser. To determine the shipment method for instruments shipped from the San Jose,
California site, check the sales agreement or the sales quote.
Table 10 lists the party who files a damage claim against the carrier for instruments damaged
in transit on the basis of the shipment method that was used to ship the instrument from the
San Jose, California site.
Table 10. Shipment methods for domestic and international shipments
Destination
Shipment method
Domestic
Destination or Origin—carrier paid by
(United States) Thermo Fisher Scientific
International
28
Party responsible for filing a
damage claim
Thermo Fisher Scientific
Origin—carrier paid by Purchaser
Purchaser
Carriage Paid To (CPT) named
destinationa
Purchaser
Carriage and Insurance Paid (CIP) to
named destinationb
Thermo Fisher Scientific
a
Unless specified differently, Thermo Fisher Scientific uses this shipment method for international shipments.
b
Under special circumstances, Thermo Fisher Scientific uses this shipment method for international shipments.
EASY-nLC 1200 Preinstallation Requirements Guide
Thermo Scientific
8
Instrument Arrival
Shipment Methods
Domestic Shipments
Thermo Fisher Scientific ships instruments from the San Jose site to destinations in the
United States in these ways:
• Destination or Origin—carrier paid by Thermo Fisher Scientific
Any damage incurred in shipment is the responsibility of Thermo Fisher Scientific, and
Thermo Fisher Scientific files a claim against the carrier.
• Origin—carrier paid by the Customer
Any damage incurred in shipment is the responsibility of the purchaser, and the purchaser
files a claim against the carrier
International Shipments
Thermo Fisher Scientific ships instruments from the San Jose site to international destinations
in these ways:
• Carriage Paid To (CPT) named destination
Unless specified differently, Thermo Fisher Scientific uses this shipping method to ship
instruments from the San Jose site to international destinations. Once the instrument
reaches the named destination, any damage incurred in shipment is the responsibility of
the purchaser, and the purchaser files a claim against the carrier.
• Carriage and Insurance Paid (CIP) to named destination
Under special circumstances, Thermo Fisher Scientific uses this shipping method to ship
instruments from the San Jose site to international destinations. Any damage incurred in
shipment is the responsibility of Thermo Fisher Scientific, and Thermo Fisher Scientific
files a claim against the carrier.
Thermo Scientific
EASY-nLC 1200 Preinstallation Requirements Guide
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I
Index
A
F
acetonitrile, solvent requirements 22
air conditioning load 10
fans 10
FCC compliance vii
filtering solvents 21
firewalls, network 6
formic acid, solvent requirements 22
freight insurance 27
B
best practices
ordering pre-mixed solvent blends 21
using power conditioners 15
blending solvents 21
buck/boost transformer kit 15
G
grounding requirements 17
C
H
Carriage Paid To (CPT), international shipments 29
compliance
FCC vii
regulatory vii
WEEE ix
computer damage, cause of 17
consumable parts 24
contact closure cables, ordering 25
contact information
Customer Service (U.S., Canada) xv
Technical Support xv
worldwide service offices v–vi
HPLC-grade solvents 21
humidity, operating environment 10
D
dimensions
EASY-nLC instrument 4
shipping containers 3
documentation, accessing xiv, xvi
E
electrical outlets 16
electrostatic discharge requirements 11
ESD requirements 11
Thermo Scientific
I
installation request form iii, 23
insurance, freight 27
Internet access 6
ion source, nanospray 4, 25
isolation/noise-suppression transformer 15
L
LC/MS-grade solvents 21
lighting requirements 10
line power
electrical outlets 16
monitoring 15
nanospray ion source 16
power conditioning devices 15
quality 14
requirements 13, 18
line voltage, operating range 17
EASY-nLC 1200 Preinstallation Requirements Guide
31
Index: M
M
maintenance 26
manuals, customer xiii, xvi
mixing solvents 21
MS detector, proximity of 4
N
network
cards 4
firewalls 6
noise and interference, cause of 17
telephone 6
temperature, operating environment 9
training 26
U
uninterruptible power supply 18
URLs, consumable parts xvi
USB flash drive with manuals xiii
V
ventilation system 19
vibration, floor 10
O
operating environment
electrostatic discharge 11
humidity 10
particulate matter 10
outlets, electrical 16
W
waste solvents, disposing of 19
water, solvent requirements 22
WEEE compliance ix
weight, EASY-nLC instrument 4
P
particulate matter requirements 10
port 22, firewall setup for 6
power
monitoring devices 15
quality of 15
purification system, water 21
R
regulatory compliance vii
replacement parts 25
S
safety and special notices xiv
safety standards vii
Secure Shell (SSH) network protocol 6
shipments
damaged equipment 4
domestic 29
international 29
site preparation 3
solvents
filtering 21
installation 21
space requirements 3
spare parts 24
T
technical assistance 18
32
EASY-nLC 1200 Preinstallation Requirements Guide
Thermo Scientific