Carrier | 5358 | Human Interleukin-10 (hIL-10)

Sterile
Human Interleukin-10 (hIL-10)
(Mammalian Derived)
n SC
n SF
n LC
n LF
#5358
10 µg
(With Carrier)
10 µg
(Carrier Free)
50 µg
(With Carrier)
Orders n 877-616-CELL (2355)
orders@cellsignal.com
Support n 877-678-TECH (8324)
info@cellsignal.com
Web n www.cellsignal.com
50 µg
(Carrier Free)
Multi-milligram quantities available
New 07/10
This product is intended for research purposes only. This product is not intended to be used for
therapeutic or diagnostic purposes in humans or animals.
Source: Recombinant human IL-10 (hIL-10) Ser19 Asn178 (Accession # NP_000563) was expressed in human
293 cells at Cell Signaling Technology.
Molecular Characterization: Recombinant hIL-10
contains no “tags” and has a calculated MW of 18,647.
DTT-reduced protein migrates as an 18 kDa polypeptide and
non-reduced protein migrates as a 16 kDa polypeptide due
to intramolecular cystines. The expected amino-terminal
SPGQG of recombinant hIL-10 was verified by amino acid
sequencing.
Endotoxin: Less than 0.01 ng endotoxin/1μg hIL-10.
Purity: >98% as determined by SDS-PAGE of 6 μg
reduced (+) and non-reduced (-) recombinant hIL-10. All
lots are greater than 98% pure.
+
–
100
80
Phospho-Stat3
(Tyr705)
60
50
30
20
140
100
Stat3
80
30
Maintain sterility. Storage at -20ºC should be in a manual defrost
freezer.
0
hIL-10 (ng/ml)
Western blot analysis of extracts from RPMI 8226 cells,
untreated or treated with hIL-10 for 10 minutes, using PhosphoStat3 (Tyr705) (D3A7) Rabbit mAb #9145 (upper) or Stat3
Antibody #9132 (lower).
22
hIL-10
14
20
0
37
31
50
10
0
20
66
55
6
4
The purity of recombinant hIL-10 was determined by SDS-PAGE
of 6 µg reduced (+) and non-reduced (-) recombinant hIL-10
and staining overnight with Coomassie Blue.
Bioactivity: The bioactivity of recombinant hIL-10 was
determined in a MC/9 cell proliferation assay. The ED50 of
each lot is between 0.3-1.4 ng/ml.
650
450
OD -OD
© 2010 Cell Signaling Technology, Inc.
0.3
0.2
0.1
0
0.001
0.01
0.1
1
10
hIL-10 (ng/ml)
100
1000
Applications: Optimal concentration for the desired application
should be determined by the user.
Background: IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells and macrophages (1,2). IL-10 initiates
signal transduction by binding to a cell surface receptor complex
consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads
to the activation of Jak1 and Tyk2, which phosphorylates Stat3
(1,3). The anti-inflammatory activity of IL-10 is due to its ability
to block signaling through other cytokine receptors, notably
IFNγ receptor, by upregulating expression of SOCS1 (1,3). In
addition, IL-10 promotes T cell tolerance by inhibiting tyrosine
phosphorylation of CD28 (4,5). IL-10 is an important negative
regulator of the immune response, which allows for maintenance
of pregnancy (1). In contrast, increased IL-10 levels contribute to
persistent Leishmania major infections (6).
Background References:
(1) Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
(2) Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
The proliferation of MC/9 cells treated with increasing
concentrations of hIL-10 in the presence of 1 pg/ml mouse IL-4
#5208 was assessed. After 72 hours treatment with hIL-10, cells
were incubated with a tetrazolium salt and the OD450-OD650 was
determined.
0.4
Carrier free: Add sterile PBS, or PBS containing protein to minimize absorption of hIL-10 to surfaces. Solubilize for 30 minutes
at room temperature with occasional gentle vortexing. Stock
hIL-10 should be greater than 50 μg/ml.
Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.
60
50
40
116
97
Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS,
pH 7.2.
Reconstitution:
With carrier: Add sterile PBS, or PBS containing 1% bovine or
human serum albumin or 5-10% FBS to a final hIL-10 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at
room temperature with occasional gentle vortexing.
40
10
kDa
200
Formulation: With carrier: Lyophilized from a 0.22 μm filtered
solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-10.
kDa
140
(3) O’Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
(4) Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
(5) Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
(6) Von Stebut, E. (2000) Eur J Dermatol 17, 115-22.
#5358 MSDS
Material Safety Data Sheet (MSDS) for
Human Interleukin-10 (hIL-10)
(mammalian derived)
I. Identification:
IX. Physical And Chemical Properties
Product name: Human Interleukin-10 (hIL-10) (mammalian derived)
Product Catalog: 5358
CAS#: n/a
Manufacturer Supplier: Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923 USA
978-867-2300 TEL
978-867-2400 FAX
978-578-6737 EMERGENCY TEL
Appearance:
pH:
Melting Point:
Flash Point:
Boiling Point:
Freezing Point:
Volatile Organic Compounds:
Solubility in water:
lyophilized powder
data not available
data not available
data not available
data not available
data not available
data not available
soluble in water
II. Composition/Information:
X. Stability and Reactivity:
Substance Name: Interleukin-10, mammalian derived, from 293 cells
Stability: Stable under normal conditions.
Conditions to avoid: Strong oxidizing agents
Hazardous Decomposition: Data not available.
Synonym: IL-10
CAS#: n/a
XI. Toxicological Information:
III. Hazard Identification:
!! CAUTION: This product is not for use in humans. It is intended for Research
Use Only. To the best of our knowledge, the chemical, physical, and toxicological properties
of this material have not been established.
EMERGENCY OVERVIEW
OSHA: No known hazards. This substance is not classified as dangerous according to Directive 67/548/EEC.
XII. Ecological Information: No data available.
IV. First Aid Measures:
Inhalation: If inhaled, remove to fresh air. If breathing is difficult, get medical attention.
Ingestion: If swallowed, wash out mouth with water provided person is conscious. Get
medical attention.
Skin exposure: In case of contact, immediately wash skin with soap and water for at least 15
minutes. Remove contaminated clothing. Wash clothing before reuse.
Eye exposure: In case of contact with eyes, immediately flush eyes with water for at least 15
minutes. Get medical attention.
V. Fire Fighting Measures:
Flash Point: Data not available.
Autoignition Temperature: Data not available.
Explosion: Data not available.
Fire extinguishing media: Water spray, dry chemical, alcohol foam, or carbon dioxide.
Firefighting: Wear protective clothing and self-contained breathing apparatus to prevent
contact with skin and eyes. May emit toxic fumes under fire conditions.
VI. Accidental Release Measures: Wear appropriate personal
protective equipment. Sweep up material and avoid raising dust. Transfer to a closed chemical
waste container for disposal. Wash spill site after material has been picked up for disposal.
VII. Handling And Storage:
Store in tightly closed container at 4°C. Avoid inhalation. Avoid contact with eyes, skin, and
clothing. Wash thoroughly after handling.
VIII. Exposure Controls/Personal:
© 2010 Cell Signaling Technology, Inc.
Acute Effects: Not established.
Chronic Effects: Not established.
Potential Health Effects: Not established.
Inhalation: May be harmful if inhaled.
Skin: May be harmful if absorbed through skin.
Eyes: May cause eye irritation.
Ingestion: May be harmful if swallowed.
XIII. Disposal Considerations: Dispose of in accordance with federal,
state, local environmental regulations.
XIV. Transport Information:
DOT: Not dangerous goods.
ADR/RID: Not dangerous goods.
IMDG: Not dangerous goods.
IATA : Not dangerous goods.
XV. Regulatory Information:
Labeling according to EC Directives:
This product does not need to be labeled, in accordance with EC Directives or respective laws.
US Regulatory Information:
SARA Listed: No.
Canada (WHMIS): DSL No, NDSL No.
XVI. Other Information:
This compound is sold only for research use only. It is not for use in humans. To the best of
our knowledge, this document is accurate. It is intended to serve as a guide for safe use of this
product in a laboratory setting by experienced personnel. The burden of safe use of this material rests entirely with the user. Cell Signaling Technology, Inc., shall not be held liable for any
damage resulting from the handling of or from contact with the above product.
Ventilation System: A system of local and/or general exhaust is recommended.
Skin Protection: Wear compatible chemical resistant gloves and protective clothing.
Eye protection: Wear protective safety glasses or chemical safety goggles. Maintain eye
wash fountain and quick-drench facilities in work area.
Orders n 877-616-CELL (2355)
orders@cellsignal.com
Support n 877-678-TECH (8324)
info@cellsignal.com
Web n www.cellsignal.com
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