Calnexin Antibody - Cell Signaling Technology

Store at -20°C
Calnexin Antibody
#2433
3100 µl
n
Orders n 877-616-CELL (2355)
orders@cellsignal.com
Support n 877-678-TECH (8324)
info@cellsignal.com
Web n www.cellsignal.com
(10 western blots)
rev. 07/18/11
This product is intended for research purposes only. This product is not intended to be used for
therapeutic or diagnostic purposes in humans or animals.
Entrez-Gene ID #821
Swiss-Prot Acc. #P27824
Molecular Wt.
Source
W, IHC-P, IF-IC
Endogenous
H
90 kDa
Rabbit**
Specificity/Sensitivity: Calnexin Antibody detects
endogenous levels of total calnexin protein.
Source/Purification: Polyclonal antibodies are produced
by immunizing animals with a synthetic peptide corresponding to a sequence around Ala51 of human calnexin.
Antibodies are purified by protein A and peptide affinity
chromatography.
Ca
LN
RD
La
PA
NC
3
He
kDa
29
Background: Secretory and transmembrane proteins are
synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the
formation of disulfide bonds and amino-linked glycosylation
and folding. To help proteins fold properly, the ER contains
a pool of molecular chaperons including calnexin. Calnexin
was identified as being involved in the assembly of murine
class I histocompatibility molecules (1,2). Calnexin is a
calcium-binding protein embedded in the ER membrane
that retains the newly synthesized glycoproteins inside ER
to ensure their proper folding and quality control (3,4,5).
A lectin site on calnexin for an oligosaccharide processing
intermediate defines its specificity for glycoproteins (5).
Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.
P
Species Cross-Reactivity*
1
Applications
200
140
100
80
Calnexin
60
50
Recommended Antibody Dilutions:
Western blotting
1:1000
Immunohistochemistry (Paraffin)
1:50†
Unmasking buffer: Citrate
Antibody diluent: SignalStain® Antibody Diluent #8112
Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114
†Optimal IHC dilutions determined using SignalStain® Boost IHC
Detection Reagent.
Immunofluorescence (IF-IC)1:50
30
For application specific protocols please see the web
page for this product at www.cellsignal.com.
20
Please visit www.cellsignal.com for a complete listing
of recommended companion products.
40
Western blot analysis of extracts from various cell lines using
Calnexin Antibody.
Background References:
(1) Degen, E. and Williams, D.B. (1991) J. Cell Biol. 112,
1099–1115.
(2) Ahluwalia, N. et al. (1992) J. Biol. Chem. 267,
10914–10918.
(3) Rajagopalan, S. et al. (1994) Science 263, 387–390.
(4) Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19,
124–128.
.
© 2011 Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
(5) Williams, D.B. (2006) J. Cell Sci. 119, 615–623
Immunohistochemical analysis of paraffin-embedded human
breast carcinoma using Calnexin Antibody.
Confocal immunofluorescent analysis of HeLa cells using Calnexin Antibody (green). Actin filaments have been labeled with
Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5®
#4084 (fluorescent DNA dye).
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4°C with gentle shaking, overnight.
Applications Key:
W—Western
Species Cross-Reactivity Key:
IP—Immunoprecipitation
H—human
M—mouse
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans
IHC—Immunohistochemistry
R—rat
Hr—Horse
Hm—hamster
ChIP—Chromatin Immunoprecipitation
Mk—monkey
All—all species expected
Mi—mink
C—chicken
IF—Immunofluorescence
F—Flow cytometry
Dm—D. melanogaster X—Xenopus
Z—zebrafish
Species enclosed in parentheses are predicted to react based on 100% homology.
E-P—ELISA-Peptide
B—bovine