Thermo Fisher Scientific AmpFLSTR NGM SElect Express PCR Amplification Kit User Guide

Thermo Fisher Scientific AmpFLSTR NGM SElect Express PCR Amplification Kit is a powerful tool for forensic and paternity testing. This STR multiplex assay is optimized for direct amplification of single-source samples, including blood and buccal samples obtained from treated or untreated paper substrates or buccal swabs. This kit allows for efficient and accurate analysis of DNA profiles using multiple STR loci and the sex-determining marker Amelogenin.

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AmpFLSTR NGM SElect Express PCR Amplification Kit User Guide | Manualzz

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Key features

  • Direct amplification of single-source samples
  • Amplifies 16 autosomal STR loci and Amelogenin
  • Optimized for treated or untreated paper and swab substrates
  • Validation for forensic and paternity testing
  • Compatible with Applied Biosystems Genetic Analyzers
  • Improved primer synthesis and purification
  • Non-nucleotide linkers for efficient separation
  • Five-dye fluorescent system for simultaneous amplification

Frequently asked questions

The kit is optimized for single-source samples, including blood and buccal samples collected on various substrates like treated or untreated paper, and buccal swabs.

The kit amplifies 16 autosomal STR loci (D10S1248, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D22S1045, D19S433, TH01, FGA, D2S441, D3S1358, D1S1656, D12S391, and SE33) and Amelogenin.

The NGM SElect™ Express Kit is compatible with Applied Biosystems® 3100/3100-Avant, 3130/3130xl, and 3500/3500xL Genetic Analyzers. It is also validated for use with Veriti® 96-Well Thermal Cycler and GeneAmp® PCR System 9700 with the silver or gold-plated silver blocks.
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