Thermo Fisher Scientific Pierce High pH User Guide

Thermo Fisher Scientific Pierce High pH is a kit designed for high-pH reversed-phase peptide fractionation, a method that separates peptides by hydrophobicity, enhancing the identification of proteins from complex samples in liquid chromatography-mass spectrometry (LC-MS) analysis. The kit includes pH-resistant reversed-phase resin spin columns and a high-pH solution, allowing for the fractionation of up to 12 proteolytically digested protein samples. This technique is particularly valuable for analyzing native, phosphorylated, or TMT-labeled peptide mixtures, leading to improved protein sequence coverage and a higher number of identified proteins compared to unfractionated samples.

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Thermo Fisher Scientific Pierce High pH Reversed-Phase Peptide Fractionation Kit Manual | Manualzz

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Key features

  • High pH peptide fractionation
  • 12 spin columns
  • pH-resistant resin
  • Optimized for LC-MS analysis
  • Increases protein identification
  • Compatible with TMT-labeled peptides
  • Simple and efficient workflow

Frequently asked questions

The Pierce High pH Reversed-Phase Peptide Fractionation Kit is designed to enhance protein identification from complex samples by separating peptides according to their hydrophobicity using a high-pH reversed-phase chromatography technique. This method optimizes the subsequent analysis of peptides by liquid chromatography-mass spectrometry (LC-MS).

The kit can be used to fractionate various types of peptide mixtures, including native, phosphorylated, and TMT-labeled samples. This versatility makes it suitable for a wide range of proteomic research applications.

Each kit provides enough materials to fractionate up to 12 proteolytically digested protein samples before LC-MS analysis.

By using the kit, you can expect to increase the number of proteins identified from complex samples due to the improved separation and analysis of peptides. This leads to greater protein sequence coverage and a higher overall identification rate.
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