Store at -20°C ETS-1 Antibody #6258 3100 µl n Orders n 877-616-CELL (2355) firstname.lastname@example.org Support n 877-678-TECH (8324) email@example.com Web n www.cellsignal.com (10 western blots) New 06/12 For Research Use Only. Not For Use In Diagnostic Procedures. Entrez-Gene ID #2113 Swiss-Prot Acc. #P14921 Applications W, IP Endogenous Species Cross-Reactivity* H, M, R Molecular Wt. 52 kDa Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Source Rabbit** Specificity/Sensitivity: ETS-1 Antibody recognizes endogenous levels of total ETS-1 protein. Source/Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. CA R8 **Anti-rabbit secondary antibodies must be used to detect this antibody. C6 OV -7 A2 kDa RL Background: ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9). 0 *Species cross-reactivity is determined by western blot. Recommended Antibody Dilutions: Western blotting 1:1000 Immunoprecipitation1:50 200 140 100 80 60 50 For product specific protocols please see the web page for this product at www.cellsignal.com. ETS-1 Please visit www.cellsignal.com for a complete listing of recommended complementary products. 40 30 20 10 Western blot analysis of extracts from various cell lines using ETS-1 Antibody. Background References: (1) Bartel, F.O. et al. (2000) Oncogene 19, 6443-54. (2) Sato, Y. (2001) Cell Struct Funct 26, 19-24. © 2012 Cell Signaling Technology, Inc. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. (3) Oettgen, P. (2006) Circ Res 99, 1159-66. (4) Dube, A. et al. (1999) Circ Res 84, 1177-85. (5) Lelièvre, E. et al. (2000) Oncogene 19, 2438-46. (6) Ghosh, S. et al. (2012) J Biol Chem 287, 15001-15. (7) Verschoor, M.L. et al. (2010) PLoS One 5, e13565. (8) Pognonec, P. et al. (1988) EMBO J 7, 977-83. (9) Yang, B.S. et al. (1996) Mol Cell Biol 16, 538-47. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Applications Key: W—Western Species Cross-Reactivity Key: IP—Immunoprecipitation H—human M—mouse Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans IHC—Immunohistochemistry R—rat Hr—horse Hm—hamster ChIP—Chromatin Immunoprecipitation Mk—monkey Mi—mink All—all species expected C—chicken IF—Immunofluorescence F—Flow cytometry Dm—D. melanogaster X—Xenopus Z—zebrafish E-P—ELISA-Peptide B—bovine Species enclosed in parentheses are predicted to react based on 100% homology.