ETS | 1 | ETS-1 Antibody - Cell Signaling Technology

Store at -20°C
ETS-1 Antibody
#6258
3100 µl
n
Orders n 877-616-CELL (2355)
orders@cellsignal.com
Support n 877-678-TECH (8324)
info@cellsignal.com
Web n www.cellsignal.com
(10 western blots)
New 06/12
For Research Use Only. Not For Use In Diagnostic Procedures.
Entrez-Gene ID #2113
Swiss-Prot Acc. #P14921
Applications
W, IP
Endogenous
Species Cross-Reactivity*
H, M, R
Molecular Wt.
52 kDa
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
Source
Rabbit**
Specificity/Sensitivity: ETS-1 Antibody recognizes
endogenous levels of total ETS-1 protein.
Source/Purification: Polyclonal antibodies are produced
by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1
protein. Antibodies are purified by protein A and peptide
affinity chromatography.
CA
R8
**Anti-rabbit secondary antibodies must be used to
detect this antibody.
C6
OV
-7
A2
kDa
RL
Background: ETS-1 is a proto-oncoprotein that belongs
to the E26 Transformation-specific Sequence (ETS) family
of transcription factors that share a unique and highly
conserved DNA binding domain (1). ETS-1 plays important
roles in vascular development and angiogenesis (2), and
vascular inflammation and remodeling (3). The target genes
of ETS-1 include receptor tyrosine kinases, MMPs, and cell
adhesion molecules (4-6). In addition, ETS-1 is involved in
regulation of energy metabolism in cancer cells (7). ETS-1
activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine
residues in the exon VII domain by CaMKII inhibits ETS-1
DNA binding activity (8), phosphorylation at its Thr38 site
by Ras activates ETS-1 (9).
0
*Species cross-reactivity is determined by western blot.
Recommended Antibody Dilutions:
Western blotting
1:1000
Immunoprecipitation1:50
200
140
100
80
60
50
For product specific protocols please see the web page
for this product at www.cellsignal.com.
ETS-1
Please visit www.cellsignal.com for a complete listing
of recommended complementary products.
40
30
20
10
Western blot analysis of extracts from various cell lines using
ETS-1 Antibody.
Background References:
(1) Bartel, F.O. et al. (2000) Oncogene 19, 6443-54.
(2) Sato, Y. (2001) Cell Struct Funct 26, 19-24.
© 2012 Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
(3) Oettgen, P. (2006) Circ Res 99, 1159-66.
(4) Dube, A. et al. (1999) Circ Res 84, 1177-85.
(5) Lelièvre, E. et al. (2000) Oncogene 19, 2438-46.
(6) Ghosh, S. et al. (2012) J Biol Chem 287, 15001-15.
(7) Verschoor, M.L. et al. (2010) PLoS One 5, e13565.
(8) Pognonec, P. et al. (1988) EMBO J 7, 977-83.
(9) Yang, B.S. et al. (1996) Mol Cell Biol 16, 538-47.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4°C with gentle shaking, overnight.
Applications Key:
W—Western
Species Cross-Reactivity Key:
IP—Immunoprecipitation
H—human
M—mouse
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans
IHC—Immunohistochemistry
R—rat
Hr—horse
Hm—hamster
ChIP—Chromatin Immunoprecipitation
Mk—monkey
Mi—mink
All—all species expected
C—chicken
IF—Immunofluorescence
F—Flow cytometry
Dm—D. melanogaster X—Xenopus
Z—zebrafish
E-P—ELISA-Peptide
B—bovine
Species enclosed in parentheses are predicted to react based on 100% homology.
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