AquaLabo TOXmini Operating Manual

Operating Manual
Version: V 1.1
Date : 2020
microLAN b.v.
Biesbosweg 2
NL-5145 PZ Waalwijk
www.microlan.nl
www.toxcontrol.com
Contents
TOXmini
Contents
Symbol Key ........................................................................................... 4
Safety Instructions ................................................................................ 5
1. Overview .......................................................................................................... 7
2. Instrument Set-Up ........................................................................................... 12
2.1.
Control Panel .......................................................................................... 13
2.2
Measuring Chamber ................................................................................ 14
2.3
Connections ........................................................................................... 15
2.4
Battery Compartment .............................................................................. 16
2.5
Power Supply .......................................................................................... 17
2.6
Software .................................................................................................. 17
3
Getting started ................................................................................................. 18
4
Software Set-up and Operation ....................................................................... 20
4.1
Set-up ...................................................................................................... 20
4.2
Operation ................................................................................................ 21
4.2.1
Display mode .................................................................................. 21
4.2.2
Input field ........................................................................................ 21
4.2.3
Selection Fields (Text) .................................................................... 22
4.2.4
Option Fields................................................................................... 23
4.2.5
The Keys ......................................................................................... 24
5
Setting the Parameter ...................................................................................... 25
5.1
Password ................................................................................................. 26
5.2
User Input (Measurement Parameters) ................................................... 27
5.3
Date/Time ............................................................................................... 30
5.4
Language................................................................................................. 31
5.5
The RS232 Port....................................................................................... 32
5.6
The LCD Display .................................................................................... 34
6
Measuring ....................................................................................................... 35
6.1
Possible Adjustments to the Start Display .............................................. 35
6.2
Measurement Procedure......................................................................... 36
7
Processing the Results .................................................................................... 38
7.1
Readouts on the Display ......................................................................... 39
7.2
Transferring Data to a PC ....................................................................... 40
7.4
Deleting Results ...................................................................................... 42
8
Maintenance .................................................................................................... 44
9
Trouble Shooting ............................................................................................ 45
10
Technical Data ............................................................................................ 46
Symbol Key
TOXmini
Symbol Key
!
4
This symbol indicates a dangerous situation. Failing to heed this
warning poses the threat of injuries or equipment damage!
+
This character indicates important information.

This character is used to indicate processing steps.

This character marks enumeration.
[]
Messages on the TOXmini's display are written in bold type
and set in brackets [ ], for example [Measurement Start].
<>
Function keys for the TOXmini are written in bold type and
placed in arrow keys <ENT> or <ON>.
Safety Instructions
TOXmini
Safety Instructions
Prescribed Usage Instructions for Use of Applications
The TOXmini is a small portable luminometer. It is designed
for bio- and chemiluminescence measurements using
reagents that generate a relatively constant light output.
Typical applications are for example: research, clinical
diagnostics and hygiene monitoring.
For Your Safety
The TOXmini has been designed and manufactured in
accordance with state-of-the-art technology and regulations
governing technical safety.
The manufacturer has taken all steps to guarantee safe
operation. The user must ensure that the instrument is set up
and installed so as not to compromise its safe usage.
Furthermore it is the operator’s responsibility to adhere to any
local laws and regulations applicable to the installation and
use of this instrument.
Instruments are factory tested and delivered in a condition
safe for operation.
This manual contains instructions and warnings that must be
followed in order to enable safe operation of the instrument!
To disconnect the instrument from power pull out the power
supply DC- connector or pull out the wall power supply from
main power.
Use only a approved power supply with a main voltage of
100V to 240V and a secondary voltage of 6VDC / 2,1A. The
negative pole is on the inside of the plug.
The main supply voltage fluctuations must not exceed +10%
of the nominal voltage (230VAC).
The instrument is designed for indoor use only.
!
5
Safety Instructions
TOXmini
The following safety instructions are to be observed
before starting the instrument and during its operation:

The instrument may only be put into operation by
authorized persons and used by properly-trained
personnel. All users of the instrument must first read the
instruction manual.

Users should only carry out maintenance and repair
procedures found in the manual. During these procedures,
no equipment other than that mentioned in the manual
should be used. All maintenance and repair work not
covered in the instruction manual is to be carried out only
by service technicians authorized by microLAN.

Make sure that the power supply provided is suitable for
your line voltage.

Always keep the sample holder clean!

Avoid electrostatic charges!
microLAN accepts no liability (also in cases of third-party
involvement) for damages caused through improper use of the
instrument.
For the safety of the user and to ensure good working
condition, the proper steps for starting, testing and maintaining
the instrument are to be followed.
6
1.Overview
TOXmini
1. Overview
The TOXmini is a small portable luminometer which is
suitable for measuring all types of ”glow” luminescence, i.e.,
bio- and chemiluminescent reactions that emit a constant light
output over a given period of time. The TOXmini is used in
 research (molecular biology, medicine, biochemistry and
pharmacy),
 diagnostics and
 hygiene monitoring.
Common applications are:

Reporter gene assays

ATP measurements, including hygiene monitoring to
detect microbial contamination

NAD(P)H- and enzyme measurements

Luminescence immunoassays and

DNA probe assays
The TOXmini is designed for rapid standard measurements
of single samples. Measurement results are given in RLUs
(Relative Light Units). These results can be classified in
3 categories by user-definition of two threshold values.
The advantages of the TOXmini in a single glance:
 High accuracy of measurement with easy readout
 Simple operation of standard applications
 Storage of different measurement protocols
 Automatic storing of the last 2000 measurements,
comprising the date, time, site identification number and
test number
 Display of stored results
 Transfer of (selected) results to a PC
 Deletion of measured results
 Operation using mains supply or rechargeable batteries
7
1.Overview
TOXmini
... and operation is so simple
The Display
After the TOXmini is switched on with the <ON> key, the Start
display appears in the Display mode. The desired line can be
selected using the arrow keys
, and the <ENT> key
activates the selected field (see figure 1.1):
If you wish to select

the Options field (for example, [Measurement Start]),
measuring starts;

the Input field (Number), the Input mode is activated and
the numerator can be modified;

the Selection field (for example, [Assay]), the Selection
mode is switched on and selections can be made from the
available options.
Selections or input are confirmed by pressing <ENT>, which
causes the return to the display mode.
Pressing <ESC> in the Display mode leads back to the menu
in the preceding level, for example, from the Start menu to the
Main menu (see figure 1.2).
Measurement Start
Assay:
ATP
Location:
1
Test:
1
Insert sample
Figure 1.1: Start display
8
Options field
Selection field
Input field
Information line
1.Overview
TOXmini
The Key Functions (also see Chapter 4.2)
Switches on the instrument. Press “ON” again the
display illumination can be switched on or off.
Switches off the instrument.
Activates the selected field: Menu field, Input or
Selection field. Input and Selection mode:
Confirms the entry or selection.
Display Mode: Move one level higher in the menu
hierarchy. Input and Selection mode: Return to
Display Mode without transferring changes.
Display mode: Select a line.
Selection mode: Page through the selection list.
Input mode: Modify number values.
Input mode: Move the cursor.
Selection mode: Select Yes/No.
9
1.Overview
TOXmini
Overview of the menus
Figure 1.2: The menu structure
The user is directly in de main menu after switching on the instrument. There the
users has a choice between the <Measure>, <Results> and <Settings> options:
10
<Measure>
This option is called up if measurements should be
carried out.
<Results>
The data administration is accessed via this option (call
up or delete measurement results, data transfer to the
computer).
<Settings>
The submenu for the setting of the instrument and test
parameters is accessed by calling up this option.
1.Overview
TOXmini
How do I proceed?
Step
Chapter
Page
1. Getting started
3
18
2. Define measurement protocol
5.2
27
3. Select measurement protocol
6.1
35
4. Measure
6.2
36
Where in the manual is the explanation found?
Function
Chapter
Page
Start display
6.1
35
Start measuring
6.2
36
Site identification
6.1
35
Test identification (in process)
6.1
35
Selecting a measurement protocol
6.1
35
Changing a measurement protocol
5.2
27
Saving measured results
6.2
36
Displaying measured results
7.1
39
Transferring measured results
5.5, 7.2
32, 40
11
2.Instrument Set-Up
TOXmini
2. Instrument Set-Up
Function, quality and reliability were the values most important
during development.
On the front panel of the TOXmini you have access to the
measuring chamber containing the sample holder and
photomultiplier (see Chapter 2.2) and the control panel (see
Chapter 2.1).
The connector RS232 for data transfer and connector for the
accompanying power supply are located on the rear panel of
the TOXmini (see Chapter 2.3).
The battery compartment is placed on the bottom .
The housing is protected against beating rain according to
IP54 and can be operated in temperatures up to 30° C.
Figure 2.1: Instrument set-up
12
2.Instrument Set-Up
TOXmini
2.1.
Control Panel
The control panel contains 6 function keys that operate the
software and 2 keys that control the power switch, as well as
the graphic display.
The display's background illumination is automatically
switched to the energy-saving mode when the key pad has not
been used for several seconds. It will automatically be
switched back on with the next use of the key pad. Contrast
and brightness of the display can be software adjusted (see
Chapter 5.6).
Figure 2.2: Control panel
13
2.Instrument Set-Up
2.2
TOXmini
Measuring Chamber
The measuring chamber contains both the sample holder and
the photomultiplier. It can be opened using the black button.
Only in its closed state the measuring chamber is light-tight,
which is a prerequisite for luminescence detection.
Individual test tubes are inserted into the sample holder for
measurement. Tube formats range from 12 x 47 mm to 15 x
75 mm. Use the accompanying adapter for optimal results
when measuring the smaller sized tube formats (article no.
04TM32737 Adapter vials, 15mm x 42 mm, 10 pieces).
Black button to open lid
Sample holder
Photomultiplier
Figure 2.3: Profile of the measuring chamber containing the
sample holder and photomultiplier
14
2.Instrument Set-Up
TOXmini
To open the sample holder: Press the black button.
To close: The cover should be pressed down until it clicks
into place. Only then is the chamber completely closed to light
and measurement process enabled.
The Photomultiplier (Detector)
The photomultiplier (detector) is a single photon counter that
operates in a wavelength range of 380 – 630 nm. The linear
range covers 6 decades.
The measured counts are directly displayed as RLUs.
2.3
Connections
There are two external connections. Both are located on the
rear panel of the TOXmini and can be protected with the
sliding panel:

RS232 port for transferring data to a personal computer
(serial port). A standard zero-modem cable is needed.

Female connector to connect the accompanying power
supply. The negative pole is on the inside of the plug.
RS 232
power
Figure 2.4: Connections (rear panel of the instrument)
15
2.Instrument Set-Up
2.4
TOXmini
Battery Compartment
Remove test tubes from the measuring chamber before
turning the instrument over! Danger of damage from
leaking fluids!
The battery compartment is
located on the instrument's
underside.
The compartment is covered
by a panel, which is fastened
by a screw. The three
batteries must be inserted up
as shown in figure 2.5.
A warning message is
displayed 15 minutes before
the
batteries
become
completely discharged. This
is
indicated
by
the
message: Battery empty!
The instrument then shuts
down.
For battery loading connect
the instrument to the mains
supply (see chapter 2.3).
Loading only takes place if
the instrument is switched
on. Loading time max. 4-5
hours. (see chapter 2.5)
Figure 2.5: Instrument underside with battery compartment
Use only the recommended rechargeable battery!
Ni/MH Akku Ansmann
1.2 V/4500 mAh
microLAN accepts no liability for damages caused through use
of other batteries!
16
2.Instrument Set-Up
TOXmini
2.5
Power Supply
The TOXmini operates with an input voltage of 6 V. The
instrument can be run by means of either the power supply or
the rechargeable batteries.
When the instrument is connected to the power supply and t it
is switched on, the batteries will be charged. Simultaneously,
the TOXmini can be operated.
The built-in real-time clock is maintained by means of a lithium
battery (durability – min. 5 years – lasts usually approx. 10
yrs).
Battery type
Operating time without mains supply
Charging time while operating
Ni/MH 4500mAh
6h
4 – 5 h.
Frequent partial depletion and recharging of batteries,
together with aging, lowers their capacity. In order to reduce
this, batteries should be completely discharged and recharged
at regular intervals.
A warning message is displayed 15 minutes before the
batteries become completely discharged. This is indicated by
the message: Battery empty! The instrument then shuts
down.
Use only the recommended rechargeable batteries!
2.6
Software
The user software is set up in a series of menus accessed and
controlled by using 6 function keys. The software, parameters,
and up to 2000 measurements are stored in the non-volatile
memory.
The software will recognize overload conditions, that could be
caused by excessive light emissions from a sample and
display the warning message: Overload.
For software operation, see Chapter 4.
17
3.Getting started
TOXmini
3 Getting started
Unpacking
o Unpack the instrument and power supply and check the instrument
for damage. Immediately report any problems to the distributor or
manufacturer.
Connecting and Switching On
o
o
o
o
Slide the protective panel on
the rear of the instrument to
the open position to access
the connections (see figure
3.1).
Connect the power supply to
a suitable mains outlet and
plug the in line socket to the
power inlet. The internal
batteries will start to be
charged if the instrument is
switched on.
Switch on the instrument by
pressing the <ON> key.
A few seconds after being
switched on, the display will
first show the instrument
model number together with
the software version, followed
by the Start display (see
Figure 3.2).
Measurements are started
here.
18
RS232
Power
Figure 3.1: TOXmini rear panel
3.Getting started
TOXmini
Switching on the instrument in battery mode:
If the TOXmini is not connected to the power supply, simply
press the <ON> key.
Measurement Start
MAesss
sa
uny:g S ta r t ATP
cation: A T P1
ALsosay:
OTe
rt:st:
11
T es t:
1
Insert sample
P ro b e ein fü h ren
Figure 3.2: Start display
19
4. Software Set-up and Operation
TOXmini
4 Software Set-up and Operation
4.1
Set-up
The TOXmini's software is set up for easy measurement of
standard applications.
After switching on the instrument the measurement menu
Measurement Start appears on the display. A measurement
can be started immediately by pressing the <ENT> key.
In order to define the measurement parameters or the data
output, the user simply changes to the Main menu, where the
other menus can be selected by pressing the <ESC> key:
Assay:
Location
Test:
Measurement
menu
Assay:
Location
Test:
ATP
1
1
ENT
ESC
ENT
LB 9509
Main menu
Results
V 1.0
Measurement
Results
Settings
ENT
Password
Customer Settings
Date/Time
Language
RS232
System
Figure 4.1: Software set-up
20
RLU:
Result:
insert sample
ENT
ATP
1
1
Screen
PC
Delete
111
pass
4. Software Set-up and
TOXmini
Operation
4.2
Operation
The instrument is operated by use of 8 function keys. The
readout follows on a graphic display.
Results are given in Relative Light Units (RLUs), which
means an integrated value over the measurement period. This
RLU value is assembled by the measurement of RLU values
in time intervals of 5 msec.
The result is classified as pass, warn or fail according to the
thresholds values set previously. Additionally, the
classification is indicated by 3 LEDs:
green
=
pass
yellow
=
warn
red (blinking) =
fail
red (blinking) =
The measurement has been interrupted
due to the opening of the sample holder.
red (blinking) =
Overload
4.2.1
Display mode
When the TOXmini is switched on, the readout is
automatically in the Display mode. The cursor may be moved
from one line to the next
by using the arrow key. This
causes either the entire line or a field to be marked.
In the Start display the following fields are used for operation
(see Figure 4.3):
Input field: to input values,
Selection field: to select text, and
Options field: to select an option or a menu.
4.2.2
Input field
When you mark an Input field (text or figure values) using the
cursor and press the <ENT> key, the Input mode will be
activated, enabling the displayed value to be changed by
means of the 4 arrow keys. The Input mode is identified by a
blinking cursor.
The blinking cursor marks the points at which changes may be
made. The cursor is moved to the next point by pressing the
key.
21
4. Software Set-up and Operation
TOXmini
The
key enables the value at the selected point to be
raised or lowered using a scale of 0–9.
Pressing the <ENT> key a second time confirms the value and
exits the input mode.
Example: Changing test number 111 to 121
Measurement Start
Assay:
ATP
Location:
1
Test:
111
ENT
insert sample
Measurement Start
Assay:
ATP
Location:
1
Test:
0111
Measurement Start
Assay:
ATP
Location:
1
Test:
0111
insert sample
insert sample
Measurement Start
Assay:
ATP
Location:
1
Test:
121
Measurement Start
Assay:
ATP
Location:
1
Test:
0121
ENT
insert sample
insert sample
Figure 4.2: Changing a value
ENT
o
Activate the Input mode with
. The last digit of the
number will be marked by the blinking cursor.
o
Press the
be marked.
o
Press the
o
key once. The second-to-the-last digit will
key once. The value will increase by 1.
ENT
Press the
key in order to confirm the entry and exit
the Input mode.
4.2.3
Selection Fields (Text)
When you mark a Selection field using the cursor and press
the <ENT> key, the Selection mode will be activated, and you
will be able to page through the selection list using the
arrow
key. The Selection mode can also be identified
by a blinking cursor.
Pressing the <ENT> key a second time confirms the entry.
The program returns to the Display mode.
22
4. Software Set-up and
TOXmini
Operation
4.2.4
Option Fields
A selected menu or a selected option can be identified by the
extended cursor block covering the entire line. If a menu or
option has been marked with the cursor, pressing the <ENT>
key activates the selected menu. The program enters the
selected menu or activates the option.
Examples (see Figure 4.1):
 When you select the [Results] menu while in the Main
menu and subsequently press the <ENT> key, the
[Results] sub-menu and its options will be displayed.
 If while in the [Results] sub-menu you select the [Time/
Date] option, the entry fields for date and time input will be
displayed.
Measurement Start
Assay:
ATP
Mo
ec
sa
s utio
n gn:S ta r t 1
L
Ae
ss
st:
ay :
A1
T1
P
T
01
O rt:
T es t:
Options field
Selection field
Input field
1
1
insert sample
Information line
P ro b e ein fü h ren
Figure 4.3: Start display
23
4. Software Set-up and Operation
4.2.5
TOXmini
The Keys
Switches on the instrument. Press “ON” again the
display illumination can be switched on or off.
Switches off the instrument.
Activates the selected field: Menu field, Input or
Selection field. Input and Selection mode:
Confirms the entry or selection.
Display Mode: Move one level higher in the menu
hierarchy. Input and Selection mode: Return to
Display Mode without transferring changes.
Display mode: Select a line.
Selection mode: Page through the selection list.
Input mode: Modify number values.
Input mode: Move the cursor.
Selection mode: Select Yes/No.
24
5.Setting the Parameter
TOXmini
5 Setting the Parameter
In the [Settings] menu, the userspecific
instrument
and
measurement
parameters
are
defined. These parameters should
be set following the first start-up of
the instrument. The procedure for
doing so is described in the next
pages.
In the Main menu, select [Settings]
and press the <ENT> key. This
causes the options for the
parameter to be displayed.
Measurement Start
Assay:
ATP
Location:
1
Test:
1
insert sample
ESC
LB 9509
V 1.0
Measurement
Results
Settings
LB 9509
V 1.0
Measurement
Results
Settings
ENT
Settings
Password
Customer Settings
Date/Time
Language
RS232
System
Figure 5.1:
Selecting the menu
[Settings]
25
5.Setting the Parameter
5.1
TOXmini
Password
If you want only authorized persons to change parameter
settings and/or delete results, it is possible to define a
password (max. 4 characters). Upon delivery, the TOXmini's
password has been defined as ”0000”. If this preset password
is not changed, each password query can be bypassed by
using the <ENT> key.
Changing the Password
o
In the [Settings] sub-menu,
choose the [Password] option.
o
Press the <ENT> key.
Settings
Password
Customer Settings
Date/Time
Language
RS232
System
ENT
o
Enter the old password.
o
Confirm the entry with the <ENT>
key. The cursor moves to the [New
Password] line. The Input mode is
automatically activated.
Enter the new password.
Confirm the entry with the <ENT>
key. The new password will be
accepted, and the program
returns to the [Settings] submenu.
Password
o
o
Old Password: 0000
New Password:
0
Figure 5.2:
Password entry
The sequence described above can be interrupted at any time
by pressing <ESC>. The old password is still valid, and the
program returns to the [Settings] sub-menu.
26
5.Setting the Parameter
TOXmini
5.2
User Input (Measurement Parameters)
The measurement parameters are defined in the [User Input]
sub-menu. A total of up to 6 different programs containing
measurement parameters, respectively protocols can be
saved for various measurement requirements. The desired
program can then be selected with the [Assay] query in the
Start display prior to measurement start. A protocol record
contains all of the parameters that define a measurement: the
measuring time and the threshold settings.
Defining the Measurement
Parameters
o In the [Settings] sub-menu,
select the [Customer Settings]
option.
Settings
Password
Customer Settings
Date/Time
Language
RS232
System
ENT
o Press the <ENT> key.
Password
o Enter the password and press
the <ENT> key.
Password:
o (In the event that you have kept
the password ”0000”, it will
suffice to press the <ENT>
key.)
o Select the program for which
you wish to change the
parameter (for example, [ATP]).
o Press the <ENT> key. The next
screen will be displayed with
the [Error] parameter selected.
ENT
Customer Settings
Programs
ATP
Protocol 2
Protocol 3
Protocol 4
Protocol 5
Protocol 6
ENT
Assay
Figure 5.3: User Input
000 0
: ATP
Fail :
10000
Pass
:
5000
MeasTime : 10
27
5.Setting the Parameter
o
o
o
o
o
o
o
o
o
o
28
TOXmini
Press the <ENT> key in order to activate the Input mode
and enter the RLU value for the [limit fail] which will
serve as the upper/lower threshold value to classify
results as fail.
Confirm the entry with the <ENT> key.
Move the cursor to the [O.K.] parameter and press the
<ENT> key.
Enter the RLU value for the [limit pass] which will serve
as the lower/upper threshold of the range that will be
used to classify results as pass (Acceptance range).
Be aware that results that fall between the two
thresholds will be classified as warn (= Warning range).
Confirm the entry with the <ENT> key.
Move the cursor to the [Measuring Time] parameter and
press the <ENT> key.
Enter the desired measuring time in seconds and
confirm the entry with <ENT>.
When all individual parameters have been defined and
confirmed with the <ENT> key, press the <ESC> key in
order to return to the [Settings] sub-menu.
If you wish, you can select a different program (2-6) and
define its measurement parameters. Proceed as
described for [ATP].
5.Setting the Parameter
TOXmini
In accordance with the defined threshold values, the results
will be displayed and classified:
If [limit pass] is smaller than [limit fail], RLU values, which
are smaller than [limit pass], are classified as pass.
If [limit pass] is higher than [limit fail], RLU values, which are
higher than [limit pass], are classified as pass.
[limit pass] < [limit fail]:
Measurement
Class
Results (RLU)
Meaning
LED
RLU  limit pass
Acceptance range
green
limit pass < RLU < warn
limit fail
Warning range
yellow
RLU  limit fail
Error range
red
blinking
[limit pass] > [limit fail]:
Measurement
Class
Results (RLU)
Meaning
LED
RLU  limit pass
Acceptance range
green
limit pass > RLU > warn
limit fail
Warning range
yellow
RLU  limit fail
Error range
red
blinking
pass
fail
pass
fail
29
5.Setting the Parameter
5.3
TOXmini
Date/Time
The date and time for the internal real-time clock can be
entered or changed. All results will be saved with
corresponding time and date entries. These also serve as
selection criteria for the readout on the display, the transfer of
information to a PC, and the deletion of results.
In addition, the format for the date and time can be changed.
Procedure
o
In the [Settings] sub-menu,
select the [Date/ Time] option.
o
Press the <ENT> key.
o
o
o
o
o
o
30
Settings
Password
Einstellungen
Customer Settings
Passwort
Date/Time
Benutzereingaben
Language
Datum/Uhrzeit
RS232
Sprache
System
RS232
LCD-Anzeige
ENT
ENT
Mark the [Date] option, press the
Date/Time set:
<ENT> key, and enter the
Eingabe Datum/Zeit:
desired date as corresponds to
Date : 12.01.1998
the preset format. Confirm the
Time : :10:43
am
Datum
12.01.1998
Format :: 10:43 am
entry with the <ENT> key.
Zeit
Date : :Europe
Format
Mark the [Time] option, press the
Time : Europa
: 12h am/pm
Datum
<ENT> key, and enter the
Zeit
: 12h am/pm
desired time as corresponds to Figure 5.4:
the preset format. Confirm the Date and time entry
entry with the <ENT> key.
To change the Date and/or Time format, you should
proceed as follows:
Select option [Format]/[Date] and press the <ENT> key.
Select the date format [USA] (= 04/24/1998) or [Europe]
(= 24.04.1998) using the arrow keys. The current date entry
will automatically change to the selected format. Confirm
the selection with the <ENT> key.
Select the [Format]/[Time] option and press the <ENT>
key. Using the arrow keys, select the time format
[12h am/pm] or [24h]. The current date entry will
automatically change to the selected format. Confirm the
selection with the <ENT> key.
With the <ESC> key, return to the [Settings] sub-menu.
5.Setting the Parameter
TOXmini
5.4
Language
The TOXmini's operation language is selectable.
Procedure
o
In the [Settings] sub-menu,
select the [Language] option.
o
Press the <ENT> key.
o
Select the desired language
and press the <ENT> key in
order to confirm the selection.
This causes the program to
return to the [Settings] submenu.
Settings
Password
Customer Settings
Date/Time
Language
RS232
System
ENT
Language:
Englisch
German
Figure 5.5: Selecting the language
31
5.Setting the Parameter
5.5
TOXmini
The RS232 Port
The RS232 port on the rear of the TOXmini enables the
transfer of saved data (up to max. 2000 measurements) to a
PC. This requires a standard zero-modem cable.
The transmission parameters necessary for your computer's
configuration are to be set in the [RS232] menu. In general it
is possible to use the pre-set parameters. If the terminal
program’s settings do not match the TOXmini's transmission
parameters, adapt the program’s settings to the TOXmini’s
requirements. Otherwise no data transfer is possible.
Changing the Transmission Parameters
o
In the [Settings] sub-menu,
select the [RS232] option.
o
Press the <ENT> key.
o
The transmission parameters for
the RS232 port will be displayed.
Modify these to correspond with
those of your PC. Activate the
desired parameter with the
<ENT>
key,
select
the
corresponding value, and confirm
the selection with the <ENT> key.
Settings
Password
Customer Settings
Date/Time
Language
RS232
System
ENT
[Baud Rate]
Selection: [300], [600], [1200],
[2400], [4800], [9600],
[19200] and [38400] Baud.
[Data Bits]
Selection: [7] and [8].
[Parity]
Selection: [None], [Odd], [Even].
[Stop Bits]
Selection: [1] or [2]
[Flowcontrol]
32
RS232:
Baudrate
Databits
Parity
: 19200
: 7
: None
Flowcontrol: XON/XOFF
Figure 5.6:Defining
the transmission
parameters
5.Setting the Parameter
TOXmini
Selection:
o
[XON/XOFF] =
[Rts/Cts] =
[None] =
Pressing the <ESC> key, returns you to the [Settings]
sub-menu.
In order to transfer the selected data to your PC, first select
the [Results] sub-menu in the Main menu and then the [PC]
option. From this menu it is possible to select the data to be
transferred according to date, time, and site (see Chapter 7.2).
Please note:
Your PC must have a terminal program in order to be able to
process the transferred data.
33
5.Setting the Parameter
5.6
TOXmini
The LCD Display
Using this sub-menu, it is possible to modify brightness and
contrast on the LCD display.
Procedure
o
In the [Settings] sub-menu,
select the [System] option.
o
Press the <ENT> key.
o
Select
the
[Brightness]
System:
parameter and press the
<ENT> key. Using the arrow
Brightness : 3
keys,
the
brightness
Contrast : 7
may be set between levels 1–
4. The display illumination is
changed simultaneously to
Figure 5.7:
this entry.
Setting display brightness
Once you have adjusted the
and contrast
brightness to the desired
level, press the <ENT> key
again.
Select the [Contrast] parameter and press the <ENT>
key. Using the arrow keys, the contrast level may be
adjusted between levels 1–10.
Once you have adjusted the contrast as desired, press
the <ENT> key again.
By pressing the <ESC> key, you return to the [Settings]
sub-menu.
o
o
o
o
34
Settings
Password
Customer Settings
Date/Time
Language
RS232
System
ENT
6. Measuring
TOXmini
6
Measuring
To start a measurement in the Measure menu choose the
[Measurement Start] option and press <ENT>.
Parameters for the measurement can be selected and/or set
in the Measure menu. This is achieved by moving the cursor
to the appropriate line using the arrow keys
and
activating the Input and/or the Selection mode with the <ENT>
key. Changes are made by using the 4 arrow keys.
6.1
Possible Adjustments to the Start Display
[Assay]
The desired measurement protocols can
be selected from 6 variations. These are
prepared in the [Settings]/ [User Input]
menu.
[Location]
An identification number for the
location (site) can be entered.
[Test]
An identification number for the
measurement can be entered. This
number will be automatically raised by
1 following each measurement, but can
be changed as the user desires.
Measurement Start
Assay:
ATP
Location:
1
Test:
1
insert sample
Measurement Start
Assay:
Protocol 1
Location:
1
Test:
1
insert sample
:
Measurement Start
Protocol 6
Assay:
Location:
1
Test:
1
insert sample
Figure 6.1: Selecting the desired measurement protocol
35
6. Measuring
6.2
o
o
o
o
o
o
o
36
TOXmini
Measurement Procedure
Prepare the sample according
Measurement Start
Assay:
ATP
to the reagent manufacturer’s
Location:
1
instructions.
Test:
1
Select
the
desired
insert sample
measurement protocol and if
necessary, enter a location, as
ENT
well as a test identification
number.
Assay:
ATP
Open the cover of the
Location:
1
Test:
1
measuring chamber and insert
Time:
6,5,4...
the test tube into the sample
holder.
Close the measuring chamber.
ENT
The cap must click into place.
The measuring chamber will
Assay:
ATP
then be light tight and the
Location:
1
Test:
1
measurement can be started.
RLU:
111
At the [Measurement Start]
Result: pass
menu option, press the <ENT>
key.
The measurement will starts.
The remaining measuring time Figure 6.2:
in seconds is displayed while Measurement procedure
the measurement is being
taken.
On completion of the measurement the RLU value is
displayed. The measurement will also be classified
according to the threshold value settings (pass = O.K.,
fail = Error range or warn = Warning range) and
indicated by the illumination of the appropriate LED.
6. Measuring
TOXmini
Automatic Storing of Results
The results are saved sequentially. If the memory is full (after
the 2000th measurement), the oldest measurement data will
be lost.
Stopping a Measurement
o
A measurement in progress can be stopped by pressing
the <ESC> key. A safety query appears asking if you are
certain that you would like to abort the measurement.
o
Using the arrow keys
, select [YES] or [NO] and
confirm your answer using the <ENT> key. The
measurement will be aborted following selection of the
[YES] key, and the program returns to the Start display.
Following selection of the [NO] key, the measurement
continues.
Returning to the Start Display and Restarting a
Measurement
o
To return to the Start display, press the <ENT> key
following the completion of the measurement. This
causes a return to the Start display and automatically
raises the test number by 1. You may immediately start
taking a new measurement.
37
7. Processing the Results
TOXmini
7 Processing the Results
There are several options available in the [Results] menu for
processing the stored results:

The results, selected using Time and Site, may be
transferred to a PC.

The results, selected using Time and Site, may be shown
on the LCD display.

Selected results can be deleted.
Choosing the [Results] menu
o
o
When the readout shows the
Start display, press the <ESC>
key.
Measurement Start
Assay:
ATP
Location:
1
Test:
1
insert sample
The Main menu appears.
ESC
LB 9509
o
Select the [Results] menu.
o
Press the <ENT> key.
o
The options for processing the
results will be displayed.
V 1.0
Measurement
Results
Settings
ENT
Results
Screen
PC
delete
Figure 7.1:
[Results] menu
38
7. Processing the Results
TOXmini
7.1
Readouts on the Display
The results can be selected using the time of measurement
and the site identification number and subsequently shown
one after the other on the LCD display.
Procedure
o
o
o
o
o
o
Select the [Screen] option.
Press the <ENT> key.
The [Display by starting at]
display is shown with the
selection criteria. Automatically
the actual date, time and the site
“0” are presented. If nothing is
changed, the oldest result is
shown.
Enter [Date] and [Time] as the
starting points. This causes all
measurements taken between
the entered point in time and the
current date to be selected for
readout.
As a further criterion for selection,
enter the [Site] identification
number for the measurements
that should be displayed. If you
would like to display the
measurements
independently
from the site identification, enter
”0”.
Select [Start Search] with the
cursor and press the <ENT> key.
Results
Screen
PC
delete
ENT
Display by
Starting at:
Date
:12/28/1997
Time
: 06:20 pm
Location :
1
Start search
ENT
Assay:
ATP
Date:
12/28/1997
Time:
06:20 pm
Location:
1
RLU:
Result:
111
pass
Figure 7.2:
Displaying results
o
This causes the first (= earliest in
terms
of
time)
result
corresponding to the selection
criteria to be shown.
o
o
Page further with the
key.
Pressing the <ESC> key returns you to the [Results]
menu.
39
7. Processing the Results
7.2
TOXmini
Transferring Data to a PC
The measured results can be selected using the time of
measurement and the site identification number and
subsequently transferred to a PC.
Procedure:
o
o
o
o
o
o
o
o
o
40
Connect the RS232 port on the
Results
rear side of the instrument with the
Screen
serial port of your PC and start
PC
your
terminal
program
(see
delete
Chap. 2.3).
On the TOXmini’s display, select
the [PC] option.
ENT
Press the <ENT> key.
Display by
The [Display by Starting Point at]
Starting at:
display is shown with the selection
criteria. Automatically the actual
Date : 12/28/1997
Time
: 06:20 pm
date, time and the site “0” are
Location :
1
presented. If nothing is changed,
start search
the oldest result is transferred.
Enter [Date] and [Time] as the
ENT
starting point. This causes all
measurements taken between the
Transfer of data
entered point in time and the
current date to be selected for
transfer.
As a further criterion for selection,
enter the [Site] identification
number for the measurements that
should be transferred. If you would
Figure 7.3:
like to display the measurements
Transferring results
independently
from
the
site
to a PC
identification, enter ”0”.
Select [Start Search] with the cursor and press the
<ENT> key.
This causes all results corresponding to the time and site
criteria to be transferred to the PC. The message
[Transfer of data] will appear on the display.
Pressing the <ESC> key returns you to the [Results]
sub-menu.
7. Processing the Results
TOXmini
Format of Data Transfer
a) Data transfer to PC
Data is transferred in the following order:
Date
Time
Program
Limit pass
Limit fail
Measuring time
Location identification number
Test identification number
Result (= integrated RLUs) or ”Overload”
<CR> (= carriage return)
<LF> (= line feed)
If an overload occurs during a measurement, “Overload” is
transferred instead of a result (RLU value).
Each value is separated by using <TAB>.
Example:
03/23/98<TAB>08:32:58<TAB>ATP<TAB>10000<TAB>5000
<TAB>10<TAB>1<TAB>3<TAB>1556<TAB><CR><LF>
b) On-line data transfer to PC
Here, data are transferred in the same format and sequence
as in a) however, additionally 100 single RLU values are
transferred, which are positioned in front of the integrated
RLU value. Also the single RLU values are separated by
using <TAB>.
If the measurement is aborted, the values are transferred up
until stopping the measurement and ”CANCEL” will be
transferred.
41
7. Processing the Results
7.4
TOXmini
Deleting Results
The results to be deleted are
selected using the time of measurement as well as the site identification
number and are shown before
deletion on the display.
Results
Screen
PC
delete
ENT
Procedure
o
Select the [Delete] option.
o
Press the <ENT> key and enter
the password. Confirm the entry
with the <ENT> key.
o
The [Display by starting at]
display is shown with the
selection criteria. Automatically
the actual date, time and the site
“0” are presented. If nothing is
changed, the oldest result is
deleted.
o
Enter [Date] and [Time] as the
starting points. This causes all
measurements taken between
the entered point in time and the
current date to be selected for
deletion.
o
As a further criterion for selection,
enter the [Site] identification
number for the measurements
that should be displayed. If you
would like to delete the
measurements
independently
from the site identification, enter
”0”
o
Select [Start Search] with the
cursor and press the <ENT> key.
Password
Password: 0000
ENT
Display by
Starting at:
ESC
Date : 12/28/1997
Time
: 06:20 pm
Location :
1
start search
ENT
Assay:
ATP
Date:
12/28/1997
Time:
06:20 pm
Location:
1
RLU:
111
Result: pass
ENT
ESC
Assay:
ATP
Date:
12/28/1997
Time:
06:20 pm
Location:
1
RLU:
111
Result: pass
Delete result ?
ENT
? Delete
Result?
Yes
No
Figure 7.4: Deleting
results
42
7. Processing the Results
TOXmini
o
This causes the first measured result corresponding to
the entered point in time and the defined site
identification to be displayed.
o
Page further with the
key until you reach the readout
of the result that you would like to display.
o
Press the <ENT> key to delete the displayed
measurement.
The safety query [Delete measurement?] will appear.
Pressing the <ENT> key again will cause the displayed
measurement to be deleted and the next measurement
to be displayed. The deletion is aborted with the <ESC>
key, and you can continue paging through the list.
In this way, you can delete measurements at your
discretion.
Pressing the <ESC> key returns you to the [Results]
sub-menu.
o
o
o
o
43
8. Maintenance
TOXmini
8 Maintenance
The TOXmini is extremely maintenance-friendly and requires
almost no (or very little) maintenance .
Please observe the following:
1. Sample Holder
 The sample holder must always be kept clean and free
from dust, in order not to spoil measuring results!
 Fluids in the measuring chamber could damage the
instrument!
 In the event that fluid enters the test chamber,
immediately remove the test tube and turn the
instrument upside down to remove the fluid before it
enter the measuring chamber! Clean the sample
holder.
 If fluid enters the measuring chamber, inform service
personnel immediately!
2. Caring for the Rechargeable Batteries
To prevent the capacities of the batteries from diminishing,
they should be completely discharged on a regular
basis (every 2–3 months) and then recharged.
Discharging duration: ca. 6 h.
Recharging duration: ca. 4–5 h.
In order to discharge the batteries, detach the TOXmini
from the power supply and leave it on for a given period of
time, for example, overnight. To recharge the batteries,
reattach the instrument to the power supply and switch it
on.
44
9. Trouble Shooting
TOXmini
9 Trouble Shooting
Error
Cause
Solution
Display - 10
HV part could be
wet
Turn off instrument
and let it dry.
Switch on again. If
error message
appears again,
contact service.
other error
messages
contact service
45
10. Technical Data
TOXmini
10 Technical Data
Detector
Sensitivity
Dynamic range
Tube format
Language
Display
Interface
Software
Measurement
time
Data storage
PC-software
Power supply
Installation category
Current consumption
Batteries
Humidity
Altitude
Temperature
Recommended
operation temperature
Pollution degree
Dimensions
(WxDxH)
Weight
46
Ultra fast single photon counter with spectral
sensitivity range of 380-630 nm
1 fmol ATP using the ATP Bioluminescence
Assay Kit CLSII (Boehringer Mannheim, FRG)
more than 6 decades
12 mm diameter, 47-75 mm height;
15 mm diameter, 47-75 mm height
English or German (software selectable)
Graphic display with backlit LED, 128 x 64
dots
RS232 C interface for data transfer to a PC
built-in microprocessor, storage of 6 different
measuring protocols
selectable in steps of 1 s between 1 and 999 s
storage of the last 2000 results
data capture software program WinTerm for
easy data transfer and evaluation
Prim.: 230V / 50Hz; 115V / 60Hz
Sec.:
6V DC, 2,1A
(Negative pole is on the inside of the plug!)
II
max. 15 VA
3 rechargeable Ni/MH Akku Baby
1.2 V / 4500 mAh
80% at 31°C degreasing linearly to 50% at
40°C no condensation
<2000m
5°C to 40°C
+15°C to +30°C
2
150 mm x 280 mm x 170 mm
approx. 2 kg net, incl. batteries
10. Technical Data
TOXmini
RS232 interface: pin configuration
Pin1
Pin2
Pin3
Pin4
Pin5
Pin6
Pin7
Pin8
Pin9
I
O
O
I
O
nc
RxD
TxD
nc.
GND
nc.
RTS
CTS
nc.
Ordering Information
TOXmini portable luminometer
Disposable Glass Cuvettes 200
Adapter vials, 15x42 mm,10 pack
Rechargeable batteries, 3 pack
PC connecting cable
Sturdy Metal Transport case
PC Terminal software WinTerm
Test Light source
Ordering number
01TM32526-10
04AZF686004-200
01TM32737
06TM40650
07TM20246
01TM32700
07TM29890
01TM11070
47