Okolab H301-PI-736-ZR1S User Manual

User Manual
H301-PI-736-ZR1S/ZR2S
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H301-PI-736-ZR1S/ZR2S
Compatible with the following Z piezo stages
• PI:P-736-ZR1S/ZR2S;
Compatible with the following Okolab Controllers
• H301-T-BL-PLUS
• UNO-COMBINED-CONTROLLER
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• H401-T-DUAL
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H301-PI-736-ZR1S/ZR2S
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Index
Components and dimensions................................................................................................... 3
2.
Insertion of the Sample Feedback Temperature Sensor .................................................................. 4
3.
Working with 35 Petri Dish- .................................................................................................... 4
4.
Working with 1”x3’’ and 1”x2’’chamber slides............................................................................ 5
5.
Connection of the Gas Supply ................................................................................................. 6
6.
Working with Perfusion ......................................................................................................... 6
7.
Connection of the Chamber with Z piezo stage ............................................................................ 7
8.
Cleaning ........................................................................................................................... 8
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1.
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1. Components and dimensions
H301-PI-736-ZR1S/ZR2S includes the following components:
• Chamber main body with embedded temperature sensor
• Lid with Indium Tin Oxide (ITO) conductive coating and embedded temperature sensor
• Chamber riser. It is a removable frame increasing the height of the chamber from 20 to 25 mm
Lid
Distance between focal plane
and upper side of the glass
lid with the chamber riser
Distance between focal plane
and upper side of the glass
lid without the chamber riser
Chamber Main Body
Chamber Riser
Chamber top view
Sample Holder-Ordered Separately
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Figure 1. H301-PI-736-ZR1S/ZR2S. Components and Dimensions.
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2. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-PI-736-ZR1S/ZR2S
chamber riser (see Figure 2, Frontal and 3D views).
Temperature Sensor dedicated opening
Temperature Sensor dedicated opening
1.Frontal view
2.3D view
Figure 2. Insertion of the temperature sensor inside the chamber.
3. Working with 35 Petri DishFlat springs prevent movement of 35 mm Petri dishes inside of the sample holder (See Figure 3).
B
A
1
2
3
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Figure 3. Plate adapter for 35mm Petri-dish holder.
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4. Working with 1”x3’’ and 1”x2’’chamber slides
Flat springs prevent movement of 1”x3’’ and 1”x2’’ chamber slides inside of the sample holder.
A
A
1
2
Figure 4. Sample holder for 1”x3” chambered cover glass holder.
A
A
1
2
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Figure 5. Sample holder for 1”x2” chambered cover glass holder.
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5. Connection of the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-PI-736-ZR1S/ZR2S. Silicon tubing
connects to a gas input - brass opening - located on a corner of the H301-PI-736-ZR1S/ZR2S. See Figure 6. Connect by
gently pushing silicon tubing onto brass opening.
Gas Input
Figure 6.Connection with gas supply.
6. Working with Perfusion
The Chamber Riser included with H301-PI-736-ZR1S/ZR2S features 12 perfusion holes for the insertion of perfusion
tubing up to 2.5 mm in outer diameter. Small screws plug the perfusion holes when not in use. (Grub screws M3x6).
Remove small screws as needed before introducing perfusion tubing.
Figure 7 shows location of perfusion holes.
Perfusion dedicated opening
1.Frontal view
2.3D view
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Figure 7. Perfusion
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7. Connection of the Chamber with Z piezo stage
Follow the steps shown in the images of Figure 8 and listed below in order to correctly connect the chamber with Z
piezo stage:
1.
Place the sample holder on the stage and tighten the four M2x3 Pan head screws, as shown in Figure 8 Image 1
2.
Place the chamber main body on the stage and tighten the two M2x3 Pan head screws, as shown in Image 2 and 3
Figure 8
3.
Place the lid on the chamber, as shown in Image 3 of Figure 8
Screw M2x3
1
2
Screw M2x3
3
4
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Figure 8. How to assemble the chamber to the stage.
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8. Cleaning
•
Turn the system off and pull the mains plug out the socket
•
Remove the lid from the chamber main body, and keep it separate from the chamber main body while the
chamber cools down.
•
To clean the body and the glass lid of the chamber, wipe with a soft micro-fiber cloth. For stubborn smudges,
you can damp the soft micro-fiber cloth with ethyl alcohol (product code UN1170). Do not put any liquid directly on the chamber. While cleaning the glass lid, do not apply strong force to the surface of the glass lid
H301-PI-736-ZR1S/ZR2S_User Manual Rev 02_Published in January 2020
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because it can be damaged.