OMEGA BIOTEK RNA-Solv reagent Owner's Manual

OMEGA BIOTEK RNA-Solv reagent is a single-reagent system for total RNA isolation from various sources, including cells and tissues. This efficient method maintains RNA integrity while lysing cells and denaturing RNases. Chloroform extraction separates RNA into an aqueous phase for simple isopropanol recovery. Suitable for diverse applications, including Northern blotting, RT-PCR (with DNase treatment recommended).

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RNA-Solv Reagent Manual: Total RNA Isolation | Manualzz

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Key features

  • Single-reagent system
  • Maintains RNA integrity
  • Wide range of sample types
  • Fast protocol (1 hour)
  • Suitable for various downstream applications

Frequently asked questions

The viewed document states it's suitable for a wide range of starting material; up to 1 g of tissue or 1 x 10⁸ cells of human, animal, plant, or bacterial origin.

According to the viewed document, RNA-Solv® Reagent is stable for at least 12 months when stored at 2-8°C.

Measure absorbance at 260 nm and 280 nm with a spectrophotometer. One OD unit at 260 nm corresponds to 40 μg/mL RNA. Dilute in TE buffer for accurate spectrophotometric analysis.

Store RNA samples at -70°C in water. Under these conditions, RNA is stable for more than a year.

The viewed document warns that the reagent is toxic if swallowed and can cause burns on contact with skin. Wear gloves and eye protection, and work in a chemical fume hood.
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