Auto-Sealing Lids Reusable Automation-Friendly Sealers for PCR Plates

Auto-Sealing Lids Reusable Automation-Friendly Sealers for PCR Plates
Auto-Sealing Lids
Reusable Automation-Friendly Sealers for PCR Plates
These lids are reusable automation-friendly sealers that prevent evaporation and
contamination during reaction assembly and seal tightly for thermal cycling when the
cycler lid is closed. Lids are constructed of metal with an attached compressible pad
(Microseal® ‘P+’ material).
When auto-sealing lids are used in combination with a Moto-Alpha™ motorized cycler lid,
automated sealing and unsealing of PCR plates can be accomplished without an expensive
heat-sealing or piercing device. The rigid metal lids may be handled with robotic arms
for full automation of lidding and delidding. The extremely tight seal produced by the
compressible pad in combination with the high-pressure motorized lid allows confident
cycling of very low-volume reactions (down to 1 µl) in 384-well PCR plates.
Both flat and arched lids are available. The arched lid automatically releases from the PCR
plate after thermal cycling, and it can then be removed by a robotic arm. Arched lids are
therefore ideal for SNP reactions that must be opened and resealed repeatedly. The flat lid
is the best choice for low-volume reactions (1–5 µl) and for storage of samples after PCR.
The flat lid must be removed manually.
Auto-Sealing Lid Selection Chart
Lid Format
Plate
Dimensions
Motorized Lid
Minimum Reaction
Volume
Motorized Lid
Pressure Setting
Manual Lid
Minimum
Reaction Volume
Feature
Flat lid
96-well
≥3 µl
1x
≥5 µl
384-well
≥1 µl
1x
≥2 µl
Remains sealed
for postcycling
storage
96-well
≥5 µl
2x
≥6 µl
384-well
≥1 µl
2x
Not recommended
Arched lid
Auto-Sealing Lids, Flat
MSL-2012 Contains four metal lids with attached sealing pads. Reusable up to 50 times.
For sealing PCR plates before, during, and after cycling.
Auto-Sealing Lids, Arched
MSL-2022 Contains four metal lids with sealing pads. Reusable up to 50 times. Self-releasing lids, for sealing
PCR plates before and during cycling.
MSL-2032 Wide tab. Lids have wide tabs along two edges to provide a larger surface for robotic grippers.
Contains four metal lids with sealing pads. Reusable up to 50 times. Self-releasing lids, for sealing
PCR plates before and during cycling.
Lid auto-releases
for repeated
access
Tips for Use
• Clean the surface of the pad before each use (e.g., wipe with a 10% bleach
solution, followed by an ethanol or ddH2O rinse)
• To ensure contamination-free reaction setup, place a lid on each PCR plate to be used.
Remove lid just before adding reaction mixtures, and replace it immediately after to reduce
evaporative loss and the possibility of contamination. If the lid is removed before the experiment is completed, it is best to maintain the same orientation when replacing the lid, to
avoid cross-contamination of wells
• The lid serves as an effective seal during PCR. After thermal cycling, the arched lid will
automatically release from the PCR plate. The flat lid will remain tightly sealed to the
PCR plate for up to 24 hours, and can be used for short-term storage down to –20°C. To
remove the flat lid, lift up at one corner and peel the lid off the plate. Before opening, check
to see if any samples are suspended above the well bottoms and, if so, briefly centrifuge
the plate to prevent aerosol generation
• For best results, use a constant lid temperature of 90°C and set the lid pressure at 1x for
flat lids, or 2x for arched lids
• If you notice any tendency for the pad to shift on the metal backing, turn the auto-sealing
lid 180° from run to run. If necessary, the pad can be peeled from the lid and repositioned
• Discard the auto-sealing lid after 50 uses
Sample remaining, % of 10 µl
Evaporation Prevention During Reaction Setup (Before Thermal Cycling)
100
100
100
100
90
90
90
90
80
80
70
70
80
96-well plate
70
Flat lid
80
384-well plate
70
Flat lid
Arched lid
Arched lid
No lid
No lid
60
60
15
30
45
60
75
90
15
30
45
60
75
90
Time after sample added to plate, min
Both flat and arched auto-sealing lids are effective at preventing reagent evaporation from PCR plates
during room-temperature reaction setup. Over 90 min, less than 3% (<0.3 µl) was lost to evaporation from
the 384-well plates, and less than 5% (0.5 µl) was lost from the 96-well plates. Hard-Shell® PCR plates
were used. Sample volume was 10 µl.
Practice of the patented polymerase chain reaction (PCR) process requires a license. Bio-Rad and MJ brand thermal cyclers and
systems include an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Their use
with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents.
Some applications may also require licenses from other third parties.
Part# 09646
US/EG
Rev D
05-0459
0905
Sig 1204
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