HESI-II Probe Quick Reference Guide Revision B

HESI-II Probe Quick Reference Guide Revision B
HESI-II Probe Quick Reference Guide
For a system with a HESI-II probe, use the recommendations below to optimize performance and the instructions
on the back of this guide to replace the HESI-II probe needle insert.
Initial
Settings
These initial tune settings are based on a 50 percent aqueous solution.
Liquid
flow rate
(μL/min)
Ion transfer
tube temp
(°C)
Vaporizer
temp
(°C)
Sheath gas
pressure
(psi)
Auxiliary gas
flow
(arbitrary units)
Spray
voltage
(V)
Typical N2 gas
consumption
(L/min)
5
240
Off to 50
5
0*
+3000 (-2500)**
<1
200
350
250 to 350
35
10
+3000 (-2500)
8
500
380
300 to 500
60
20
+3000 (-2500)
13
1000
400
500
75
20
+3000 (-2500)
17
*
For a TSQ Series mass spectrometer, the allowable range for the auxiliary gas flow is 0 to 60 units.
For an LTQ Series mass spectrometer controlled from LTQ 2.5.0 or lower, the allowable range for the auxiliary gas flow is 5 to 60 units.
For LTQ 2.5.5 or higher, the allowable range for the auxiliary gas flow is 0 to 60 units for vaporizer temperatures up to 100.00 °C and 5 to 60 units for
vaporizer temperatures above 100.00 °C.
**Negative ion mode
Adjusting the
Probe
Position
Because the probe position can affect sensitivity, follow these guidelines as a starting point.
Liquid flow rate range
(μL/min)
Front-to-back position
(micrometer lines)
Probe depth
(probe depth line)
Side-to-side position
(+1 to -1 marks)
1 to 50
50 to 2000
1.75
1.75
B
D
0
0
Y To adjust the probe position
CAUTION Because the surface of the Ion Max source housing is hot during high temperature
operation, take care when adjusting the probe position.
• Using the micrometer, adjust the front-to-back probe position.
• Using the knurled nut on the left side and the +1 to -1 markers on the top front of the Ion Max source housing,
adjust the side-to-side probe position.
CAUTION - HOT SURFACE
1
+
0
0 1
1
Side-to-side
position set
to 0
Knurled nut
Revision B 70005-97002
Copyright© 2009 Thermo Fisher Scientific Inc.
All rights reserved.
Micrometer
Micrometer set
to 1.75
• Using the A, B, C, and D
markers on the probe as a guide,
adjust the probe depth.
A, B, C, D depth markers
A
C B
D
A
C
Replacing
the Needle
Insert
B
D
The factory-assembled needle insert consists of an adjustable union, a needle guide fitting, a ferrule, an O-ring,
an ESI needle, and a metal needle. Because the needle insert is factory-adjusted, do not disassemble it.
IMPORTANT Take care to avoid operating the HESI-II probe at elevated temperatures without solvent
flow from the LC system or the syringe pump. Allowing the HESI-II probe to run dry at elevated
temperatures for extended time periods can cause blockage of the replaceable metal needle.
Replacing the metal needle insert requires a 3-mm (7/64-in.) hex wrench or ball driver.
Y To replace the needle insert
1. Remove the HESI-II probe from the Ion Max
API source housing.
2. Unscrew the fingertight fitting from the sample
inlet port.
3. Remove the metal needle insert from the probe:
a. Using a 3-mm (7/64-in.) hex wrench or ball
driver, remove the two M4 ×35-mm length,
socket head cap screws. Pull the end cover off
of the probe.
b. Unscrew the needle insert, and then pull it
out of the probe body.
4. Insert a new needle insert into the probe body.
Description
Part number
Needle insert for
high-flow applications
Needle insert for
low-flow applications
OPTON-53010
M4×35-mm length
socket head cap screws (2×)
End cover
Needle insert
Adjustable union fitting
(of the needle insert)
OPTON-53011
5. Hand tighten the adjustable union fitting (of the
needle insert) until the needle insert tip protrudes
from the probe nozzle by 1.5 mm.
6. Position the end cover on the probe body.
7. Insert the two M4 ×35-mm length, socket head cap
screws into the end cover, and then tighten them
with a 3-mm (7/64-in.) hex wrench or ball driver.
8. Reconnect the fingertight fitting to the sample
inlet port.
9. Seat the HESI-II probe in the probe port of the
Ion Max API source housing and reconnect the
vaporizer cable, 8 kV cable, and nitrogen lines.
2
1.5 mm
Probe
body
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