User manual | Learn about the benefits of IN Cell Analyzer 6000 as a highly sensitive, laser-based confocal imaging platform with adjustable aperture for the most demanding high

GE Healthcare
Life Sciences
Cell analysis redefined
Cell analysis redefined
IN Cell Analyzer 6000 high-content analysis system
IN Cell Analyzer 6000 is a super-sensitive high
performance, laser-based confocal imaging
platform designed for the most demanding
high-content assays and screens. Featuring
a novel and proprietary optical system that
incorporates an iris-like variable aperture
design and next-generation sCMOS technology,
IN Cell Analyzer 6000 enables you to optimize
for speed and image quality in challenging and
variable assays.
Proprietary optical system
Inspired by the iris mechanism of the eye, the
proprietary optical system of IN Cell Analyzer 6000
delivers a new level of control for image acquisition
to maximize performance across a broad range
of imaging conditions.
• Use in open aperture mode to maximize speed
• Use in full confocal mode for efficient background rejection
• Select any aperture setting between 1 and 3 AU to suit your
specific biology
• Optimize the degree of confocality required for each channel
of your assay with the simple click of a mouse
Use of a fixed aperture in the light path to reject out-of-plane
light that does not contribute to image quality has been the
conventional strategy for achieving confocality for many years.
While conventional confocal technology is optimal when using
high-power objectives, lower and mid-power objectives are
typically used for everyday cellular assays. The variable aperture
design of the IN Cell Analyzer 6000 optical system enables
flexibility across a range of objectives, delivering the benefits
of confocal technology across a broad spectrum of highcontent challenges.
Optical engine: Principle of operation
Enabled by the use of a next-generation sCMOS detector and
laser line-scanning technology, the optical system selectively
sensitizes a defined region of the detector surface to stringently
control the capture of reflected photons that compose the image.
Hardware-based confocal systems have aperture geometries
defined by the physical dimensions of the light path, while the
optical system of IN Cell Analyzer 6000 enables the aperture
IRIS Optical Engine: Principle of operation
width to be configured between 1 and 3 AU.
Field of view
Sample
Laser module
Objective
Scanner
Collimated laser beam
Optical filters
Scanning mirror
Tuble lens
CMOS chip
Fully adjustable imaging
Extending the reach of high-throughput imaging
Settings may be adjusted along the widefield to confocal
spectrum for each channel of an assay, maximizing user
control of speed and image quality to suit each assay.
IN Cell Analyzer 6000 extends the reach of automated imaging
into samples and assays that are typically challenging to perform
in a high-throughput format.
• Flexible imaging for multiplexed assays: Multiplexed assays that
encompass multiple signals of different intensity can be managed
comprehensively for both signal quality and throughput. Optimal
imaging conditions can be selected for each individual signal,
enabling capture of all the assay biology.
• Sensitivity for imaging dim assays: The use of a highly sensitive,
low-noise sCMOS-based camera coupled with laser-based
illumination provides tremendous sensitivity to weak signals. This
generates high-quality data from signals that would typically be
lost in the noise.
Open aperture imaging: Non
confocal setting used to image
EGFP-2× FYVE cell line. (Hoechst™
33342 nuclei stain and Texas Red™
phalloidin staining).
A
Confocal imaging for thick and 3D
samples: Middle section of 60-µm thick
sample of Cytiva™ Cardiomyocytes,
stained with Cy™5 labeled antitroponin I and Hoechst 33342.
B
• Versatility for a range of sample types: Whether imaging 3D
biological structures, low-abundance endogenous biomolecules,
or co-localized proteins of interest, IN Cell Analyzer 6000 delivers
the control and performance to adapt assays more typically
associated with low-throughput confocal microscopy to a highthroughput format.
A
Selectable aperture imaging for cells in medium with strong fluorescent
background: EGFP-2× FYVE cell line with Hoechst 33342-nuclear stain and free
FITC in media. (A) Field-of-view captured in non-confocal (open aperture) mode and
(B) Same field-of-view captured using confocal mode, with reduced background.
Throughput without compromise
IN Cell Analyzer 6000 is also designed to enable increased assay
throughput through a range of features.
B
3D sectioning of Cy5 labeled CHO cells on Cytodex™ beads at 40× magnification
imaged using (A) confocal or (B) widefield modes.
Image acquisition flexibility
• Configurable confocality to balance speed and image quality to
specific requirements.
IN Cell Analyzer 6000 includes a series of user-friendly image
acquisition features that improve workflow and make image
acquisition simpler.
• A 5.5 Mp large field-of-view sCMOS camera, almost 4× larger than
traditional 1.4 Mp CCD cameras, so fewer images need to be
acquired to capture statistically robust data, saving time.
• Preview scan: Quickly preview your sample at any magnification
before starting an acquisition run. This avoids the need to image
unwanted areas and can significantly increase speed.
• A high-sensitivity next-generation sCMOS detector chip, with
typically 5× less noise than traditional 1.4 Mp CCD cameras,
delivers increased sensitivity and reduces exposure times.
• Manual microscope mode: A smooth transition from manual
microscopy to automated, high-content analysis, for easy
optimization of imaging conditions.
• Fast, precision stages that reduce non-imaging time.
• Whole-well imaging: Rapid capture of an entire well with a single
image (96-well plate) for imaging whole wells, large tissue sections,
small organisms, and rare events.
Configure your system
IN Cell Analyzer 6000 can be configured to your specific needs
by choosing from a range of optional features:
• Environmental control
• Temperature control
• Liquid handling
• Slide imaging
• Transmitted light imaging (brightfield, DIC, and phase contrast)
• Review scan intelligent scanning feature. A faster way to
automatically collect and analyze images by re-directing imaging to
only qualifying wells at a higher magnification using the specific
parameters you are looking for.
• High-density plate/array scanning: Fine-tune the well grid
alignment to facilitate scanning custom plates and arrays that might
be warped or not perfectly aligned.
• Easy optimization of the laser autofocus: This enables you to
identify differences between existing plate-maps and actual bottom
thickness then adjust the maps to prevent autofocus failure.
Technical information
Optical system
Proprietary epi-fluorescence based line scanning
confocal system. Allows image acquisition in confocal,
open aperture, and transmitted light modes.
Focus
Contrast-based software autofocus
Laser confocal hardware autofocus
Z stage resolution: 25 nm
Camera
sCMOS 5.5 Mp camera (2560 × 2160 pixels)
Field-of-view at 10×: 2.78 mm2
Cooling: 5ºC
Pixel size: 6.5 µm
Plate positioning
500 nm resolution
Plate compatibility
Compatible with 6-, 12-, 24-, 48-, 96-, 384-, and
1536-well microplates
Slide compatibility
1 × 3 in. standard microscope slide
Image acquisition
features
Preview scan capability
Whole-well imaging capability
Manual microscope mode
Optional hardware
modules
Environmental control
Slide handling
Temperature control
Liquid handling
Transmitted light imaging (brightfield, DIC,
and phase contrast imaging)
Computer
Windows® XP professional workstation
Dimensions
645 × 1140 × 605 mm (dwh)
Weight: 50 kg
Light sources
Imaging modes
Objectives
Four lasers (405, 488, 561, and 642 nm)
LED for transmitted light imaging
Line confocal (confocal aperture adjustable
from 1 to 3 AU)
Open aperture (widefield)
Transmitted light (brightfield, DIC,
and phase contrast imaging)
Supplied with 10×/0.45 and 20×/0.45
Other objectives available: 2×/0.1, 4×/0.20, 40×/0.60,
60×/0.95, 100×/0.9
Objective changer and Automated
spherical aberration
adjustment
Filter selection
Software compatibility IN Cell Investigator
IN Cell Miner HCM
Four laser blocking filters
Up to eight emission filters
Automation options
Compatible with commercially available laboratory
automation systems
Ordering information
Product
Code number
IN Cell Analyzer 6000
IN Cell Analyzer 6000 Imaging System
29-0433-23
IN Cell Analyzer Environmental Control Module
28-9534-85
IN Cell Analyzer Liquid Handling Module
28-9798-62
IN Cell Analyzer Temperature Control Module
28-9534-73
IN Cell Analyzer Transmitted Light Module
28-9534-87
IN Cell Analyzer 6000 Slide Handling Module
28-9544-75
IN Cell Investigator Image Analysis Software, single seat license 28-4089-71
IN Cell Analyzer 6000 Live Cell Package C (includes Temperature
Control, Liquid Handling, and Environmental Control Modules)
28-9798-74
For local office contact information, visit:
www.gelifesciences.com/contact
www.gelifesciences.com/incell
GE Healthcare UK Ltd
Amersham Place, Little Chalfont
Buckinghamshire
HP7 9NA, UK
GE, imagination at work, and GE monogram are trademarks of General Electric Company.
Cy, Cytiva and Cytodex are trademarks of GE Healthcare Companies.
Hoechst is a trademark of Hoechst GmbH Corporation, Texas Red is a trademark of
Life Technologies. Windows is a registered trademark of Microsoft Corporation.
© 2011–2013 General Electric Company—All rights reserved. First published March 2011.
IN Cell Analyzer 6000 is for research use only. Not for use in diagnostic procedures.
GE Healthcare Bio-Sciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden.
GE Healthcare Bio-Sciences Corp, 800 Centennial Avenue, P.O. Box 1327, Piscataway,
NJ 08855-1327, USA
GE Healthcare Europe GmbH, Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare Japan Corporation, Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku,
Tokyo 169-0073, Japan
28-9940-45 AD 04/2013

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