Brochure MultiNA

Brochure MultiNA
C297-E062E
MCE-202 Microchip Electrophoresis System for DNA/RNA Analysis
MultiNA
Simplifies Gel Electrophoresis
Quick Setup, Great Results
M C E - 2 02 Micro ch ip E le ctr ophoresis System for DNA/RNA Analys is
Genotyping
RNA Analysis
is
for Genetic Research
earch
ear
ch
h
Infectious
Inffectious Disease
Analysis
Microbiological Analysis
Food Analysis
Start Analysis in Just Three Steps
Page 4
Extremely simple operation. Once the analysis schedule has been created, simply load the samples and
reagents and click the Start button.
Automated Analysis From 1 to 108 Loaded Samples
Page 6
Fast analysis with up to four microchips in parallel.
Wide Range of Applications
Page 8
Widely used for genetic research applications as well as food analysis, genotyping, microbiological analysis,
infectious disease analysis, and RNA analysis.
Consumables and Options
Page 10
Specifications
Page 11
St ar t An a ly sis in J ust T hr e e S t e ps
Extremely simple operation. Once the analysis schedule has been created, simply load the
samples and reagents and click the Start button.
Outstanding Ease of Use
Step
1
Select the samples with the
mouse to register in the analysis
schedule.
Register the analysis schedule.*
* A single analysis schedule permits analysis using
multiple reagent kits.
Reagent quantity is calculated
automatically based on the
number of measurements.
Step
2
Load the samples and reagents.
Step
3
Press the Start button.
Automated
Analysis
Sample
Application
Migration
Separation
Detection
Full automation of all steps from sample application to data analysis.
Result
4
Analysis results screen is displayed.
Washing
Analysis
Solution to Your Frustration with Agarose Gel Electrophoresis
Agarose Gel Electrophoresis
Ease of operation
Requires a number of different
manual steps
MultiNA
E a s y a u t o m a t e d a n alysis
alysis
Quality of Results
• The sequence of manual operations from gel creation
to visualization takes much time and effor t.
• A s there are many steps you are tired up with the
process.
• No need to cast gels.
• Just load your samples
and reagents for
automated analysis.
• Automated cleaning
after analysis.
Data quality could be better
Objective analysis of results
• Only approximate sizes can be recognized when
comparing to a ladder pattern.
• Discrepancies from analysis to analysis make
comparisons difficult.
• Inadequate separation.
• Difficult to detec t small DNA .
• Correction by internal standard markers and
ladder standards result in the output of
highly reproducible size data.
• High-sensitivity fluorescent dyes achieve
order- of-magnitude greater sensitivity than
agarose /ethidium bromide systems.
• Good separation and clear detection of DNA
below 10 0 bp.
?bp
Band
?
Management
Organisation of results is difficult
Convenient data management
• Data (photograph) organization is tedious.
• Hand-written records lead to loss
and mistakes.
• Gel images and waveform data saved as
image files.
• Viewer allows parallel
display of analysis data
from different times and
dates.
• Numerical data can be
output as a csv file for
analysis by Shimadzu
AutoFinder (option) .
Page10
ge10
Microchip Electrophoresis System for DNA/RNA Analysis
5
Automated Analysis of Up to 108 Loaded Samples
Reusable microchips and selecting the optimal reagent for each sample achieves excellent
analytical performance.
System for Automated Analysis
Reagents
Five dif ferent reagent kits are available to suit dif ferent
samples. To make operation visually simple, the reagent
holders and sof t ware screen display are color- coded to
match the reagent kit used.
Reagents
*
Reagent
holder
Sample stand
and ladder
Instrument
Au t o m a t e d A n a l y s i s
* The 96 -well PCR plate can be covered with an aluminum
sheet to prevent sample evaporation.
Permits automated analysis using parallel processing of up
to four microchips. Data for each sample can be obser ved
af ter each analysis is complete, with no need to wait for
all sample analyses to complete.
Au to m a t e d C l e a n i n g Fu n c t i o n
Microchips are rinsed with water af ter analysis is complete.
Automated cleaning can be per formed using the optional
R A Chip Cleaning Kit according to the microchip condition.
Microchips
Ex tremely fine flow channels and elec trode pat terns are
created in a quar tz substrate using MEMS* technology.
A special coating allows the microchips to be reused.
Chip stage
6
* MEMS (Micro Electro Mechanical Systems)
Displaying Analysis Results in the MultiNA Viewer
Analysis results are obtained as electronic data that can be obser ved using the MultiNA
Viewer sof tware.
The comparative view function allows data from analyses performed at different times to be
compared and analyzed on the same screen.
Sample Well Display
Displays the analysis progress
status in different colors.
Gel Image
Can be saved as image data ( jpg,
bmp, tif ).
Peak Table
Predicted size values and
concentrations can be saved to a
csv file.
Elec tropherogram
Can be saved as image data ( jpg,
bmp, tif ).
Automated Size Calculation
Low molecular marker
Ladder
High molecular marker
Size Calibration Curve
Size (bp)
Mobility corrected in this range
Time(sec)
Each reagent kit contains internal standard markers. By mixing the markers with the analysis target
( sample and ladder *1) before performing analysis, the mobility of the ladder and sample can be
correc ted. The sof tware automatically handles mobility correc tion utilizing markers, the size
calibration cur ve from the ladder peaks, and sample size predic tion. The sof tware also allows the
registration and setup of your own ladders as well as the commercially available ladders *2 .
(*1 A ladder is equivalent to markers used in agarose gel electrophoresis.
*2 Conditions, such as size and concentration, determine which ladder should be used.)
Microchip Electrophoresis System for DNA/RNA Analysis
7
W i de R a n g e o f Applica t ions
Widely used for genetic research as well as food analysis, genotyping, microbiological
analysis, infectious disease analysis, and RNA analysis.
Sample Preparation
Nucleic Acid
Extraction
Elec trophoresis Result
PCR
Application
Example
Samples
Application to Food Analysis
Purified DNA
PCR Produc ts
Detec tion
Detec tion of Allergenic Substances
Application News : No. B23
Japan was the world's earliest adopter of a labeling system for foods containing allergens. DNA analysis by
qualitative PCR can be per formed on five (wheat, buckwheat, peanuts, prawn, and crab) of the seven specified raw
materials (excluding egg and milk) .
Sample
LM 1
2
3
4
5
DNA extraction
Ion-exchange resin kit
LM
Lane
Lane
Lane
Lane
Lane
DNA Purification
PCR
Primer specific for
each sample
PCR Products
1
2
3
4
5
:
:
:
:
:
:
Ladder Marker(25bp DNA Ladder)
Wheat
Buckwheat
Peanuts
Prawn
Crab
Analysis of
PCR products
MCE-202 MultiNA
Detection of
Allergenic Substance
Application to Genotyping
Gel Images
Identification of Thunnus Using PCR-RFLP Method
Application News : No. B28
The tuna-specific genetic sequence in mitochondrial DNA is amplified using PCR. This amplified DNA is cleaved
with a restric tion enzyme and the pat tern used to identif y the tuna species.
* PCR- RFLP: ( Polymerase Chain Reac tion- Restric tion Fragment Polymorphism)
Yellowfin tuna
α Bigeye tuna
Ladder
Yellowfin tuna
α Bigeye tuna
Southern bluefin tuna
Ladder
Albacore tuna
Yellowfin tuna
β Bigeye tuna
α Bigeye tuna
PCR
Southern bluefin tuna
DNA Purification
Atlantic bluefin tuna
DNA extraction
Ladder
Sample
PCR Products
Restriction enzyme
processing
PCR-RFLP Products
MultiNA
electrophoresis
Identification of
Tuna Varieties
• Tuna Species Identification Manual (Food and Agricultural
Materials Inspection Center; Fisheries Research Agency, Japan)
8
Alu I Processing
Mse I Processing
Gel Images of PCR- RFLP Separation Patterns
Tsp 509 I Processing
Application to Multiplex-PCR
Identification of Rice Varieties
Application News : No. B30
Multiplex- PCR is per formed on four sets of samples using a variet y identification kit (from Kokken) . The rice
variet y can then be identified by comparing the pat tern obtained against pat terns for each rice variet y.
Hitomebore
Kirara 397
Kinuhikari
Akitakomachi
Koshihikari
Control
L adder
Hitomebore
Kirara 397
Kinuhikari
Akitakomachi
Koshihikari
Control
L adder
Hitomebore
Kirara 397
Kinuhikari
Akitakomachi
Koshihikari
Control
L adder
Hitomebore
Kirara 397
Kinuhikari
Akitakomachi
Koshihikari
DNA extraction
Control
L adder
Sample
Extracted DNA
Multiplex PCR by Variety
Identification Kit
PCR Products
Analysis of PCR
products-MultiNA
Acquisition of
appearance patterns
of PCR products
Collation of patterns
Identification of
Rice Varieties
Set A
Application to Multiplex-PCR
Set B
Set C
Set D
Analysis with Seeplex ® FluA ACE Subtyping Kit (from Seegene)
Sample
Nucleic Acid Extraction
Reverse Transcriptase
Reaction
Positive control
Negative control
Epidemiological s tudies to gain a detailed grasp of the virus t ype during an influenza epidemic are impor tant in
medical facilities and designated regions . The Seeplex FluA ACE Subt yping Kit allow s the detec tion of t ype A
influenza and the simultaneous detec tion of four major influenza A subt ypes ( H3 : Hong Kong ; H1: Soviet ;
H1( HA) : s wine flu ; H5 : avian flu ) . It ex tensively cover s clinical research in laboratories , examination rooms , and
infec tion control depar tment s .
4 3 2 1
Negative control
DNA
Positive control
Multiplex-PCR
PCR
4
Universal Influenza A
PCR Products
3
Swine H5
Analysis of PCR products
MCE-202 MultiNA
2
Avian H5
1
Seasonal H3
Influenza Virus
Type Evaluation
Gel Images
Seasonal H1
Electropherogram
Internal control
Application to RAPD-STS Method
Identification of Common Bean Cultivars by R APD -STS
Application News : No. B32
PCR is per formed on DNA ex trac ted from four t ypes of white kidney bean. The white kidney bean variet y can then
be identified by comparing the pat tern obtained against pat terns for each variet y.
* R APD -STS : ( Random Amplified Polymorphic DNA-Sequence Tagged Sites )
Example of RNA Analysis
Rat Total RNA Analysis
Application News : No. 6
During research using RNA , it is impor tant to continuously monitor the RNA qualit y to ensure that the RNA used is
not af fec ted by degradation by RNase. MultiNA is able to accurately recognize 18S -rRNA and 28S -rRNA based on
the calibration cur ve information acquired from the ladder.
Microchip Electrophoresis System for DNA/RNA Analysis
9
Dedi c a te d C o n su ma ble s
MultiNA Reagent Kits
Microchip
Reagent kits are designed to work optimally for different size
ranges and sample types.
P/N: 292-36000-91
Part Name: MICROCHIP, TYPE WE-C
The microchip is common to all reagent kits.
RA Chip Cleaning Kit
P/N: 292-27910-91 DNA-500 Kit (1000 analyses)
P/N: 292-27911-91 DNA-1000 Kit (1000 analyses)
P/N: 292-27911-91 DNA-2500 Kit (1000 analyses)
P/N: 292-36600-91 DNA-12000 Kit (1000 analyses)
P/N: 292-27913-91 RNA Kit (1000 analyses)
Reagent Kit Contents
P/N: 292-35925-91
Part Name: CHIP CLEANING KIT-RA
Fluorescent dye and reagent components can
be adsorbed onto the wall of the microchip
flow channel, thus reducing the separation
performance and lowering the number of
reuses. Cleaning of the microchip using the
CHIP CLEANING KIT-RA eliminates the adsorbed
components and improves (or restores) the
separation performance of the microchip.
(1) Separation buffer
(2) Marker solution (internal standard marker)
* The kits do not contain fluorescent dyes or ladders. (No ethidium bromide used.)
* The kits have a shelf life. Please use them immediately after opening.
Options
—Detection of Specific Size DNA
Shimadzu AutoFinder Optional Software for Detection of Specific Size DNA
P/N: 292-96800-02 Shimadzu AutoFinder
Shimadzu AutoFinder directly imports the MultiNA analysis results in a csv format to detect DNA of specific sizes. It enables the
simple and rapid analysis of data accumulated through large numbers of analyses in the course of daily routine work. Normally
complex manipulation of data required to evaluate the absence or presence of target bands and the detection of specific size
DNA. The Shimadzu AutoFinder is a powerful tool to support your analysis.
• Developed and manufactured by Shimadzu System Development Corporation.
Detection of Specific Bands
MultiNA Data Import Screen
Import
MultiNA
analysis
results.
• The detected bands are displayed color-coded.
Detection Parameter Setting Screen
• Select the error range and detection sensitivity.
• Select the color for bands to be detected.
10
Sp ecific ations
Sample rack
Compatible with 96-well PCR plate† and 12/8-strip PCR tube (Shimadzu recommended product)
Microchip
Quartz, 23 mm separation channel length, on-chip electrodes (insert up to four microchips)
Pretreatment
Automatic sample injection, automatic separation buffer replenishing, automatic chip cleaning
Electrophoresis Voltage
Max. rated voltage: 1.5 kV, max. current: 250 µA
Analysis Cycle time
Approx. 80 s (using four chips) * DNA standard analysis (DNA-1000/premixed).
This does not include time required for carrying out the first and final wash and initial analysis.
Detection Method
LED-excited fluorescence detector (470 nm excitation wavelength) * Class 1 LED product
Separation Size Range
25 to 500 bp (DNA-500 Kit)
100 to 1000 bp (DNA-1000 Kit)
100 to 2500 bp (DNA-2500 Kit)
100 to 12000 bp (DNA-12000 Kit)
Up to 28S rRNA (5.0 knt) (RNA Kit)
Resolution
5 bp (25 to100 bp), 5% (100 to 500 bp), 10% (500 to 1000 bp), 20% (1000 to 12000 bp)
Sizing Accuracy
±5 bp (25 to 100 bp), ±5% (100 to 500 bp), ±15% (DNA-1000, DNA-2500, DNA-12000)
Required Sample
Volume
DNA analysis: Premix mode: 2 to 10 µL (after mixing with marker solution: 6 to 30 µL)
On-Chip Mixing mode: 5 to 30 µL
RNA analysis: Premix mode: 3 to 15 µL (after mixing with marker solution: 6 to 30 µL)
In the Premix mode, the marker solution is mixed with the sample before loading in the instrument.
In the On-Chip Mixing mode, the sample and marker solution are loaded separately and mixed on the microchip under program control.
Maximum Salt
Concentration
Min. Detection Limit
Quantitation Range
Quantitation Accuracy
DNA analysis: 10mM Tris-HCI containing 125 mM KCl or NaCl max.
RNA analysis: 10mM Tris-HCI, containing 1 mM EDTA max.
DNA analysis: 0.5 to 50 ng/µL (at 10mM Tris-HCI, containing 50 mM KCl and 1.5 mM MgCl2)
RNA analysis: 25 to 500 ng/µL (total RNA), 25 to 250 ng/µL (mRNA) (10 mM Tris-HCI buffer, containing 1 mM EDTA)
DNA analysis: 0.2 ng/µL (at 10mM Tris-HCI buffer, containing 50 mM KCl and 1.5 mM MgCl2)
RNA analysis: 5 ng/µL (total RNA), 25 ng/µL (mRNA) (at 10 mM Tris-HCI buffer, containing 1mM EDTA)
DNA analysis: ±30% (at 10 mM Tris-HCI buffer, containing 50 mM KCL)
Quantitation Repeatability RNA analysis: CV 10% or less (CV 20% or less for eukaryotic-origin total RNA at concentrations of 150 ng/µL or more)
External Dimensions
W415 mm × D545 mm × H508 mm
Weight
43 kg
Power Supply
100 to 120 V, 220 to 240 (CE Marking) 300 VA max.
Note) The analysis performance specifications above are based on Shimadzu standard analysis conditions and standard samples.
Note) Reagent kits and microchips are not included as part of the MultiNA instrument’s standard accessories.
† An aluminum sheet (Shimadzu recommended product) can be applied to prevent sample evaporation.
Controller and Viewer Software
Controller
Creating analysis schedules, real-time control, automatic analysis pretreatment, automatic analysis post-treatment, automatic error
processing, analysis log management, analysis performance checks
Data Processing
Batch display/detailed display of gel images/pherograms, automatic quantitation and size prediction by size markers, data searching, data
import/export, manual editing and re-analysis
Reports
Multilevel data display, tree display of samples/files, RNA structural comparison, analysis performance check results, analysis log
Compatible PC
CPU: Intel® Pentium® III processor or its equivalent, 1 GHz min.
Memory: 512 MB min. (Windows XP), 1 GB min. (Windows 7)
HDD: 40 GB min. (free space: 1 GB min.)
Display: Resolution 1024 × 768 pixels min.
OS: Windows XP SP2 or later, Windows 7 Professional 32 bits
LAN port: 100Base-TX, single port min.
(* Expansion LAN port and LAN cable are not supplied with the instrument. Users should purchase these separately.)
Note) Control PC is not supplied with the instrument. Purchase the control PC separately.
Even if the PC model meets the conditions above, the software operation cannot be guaranteed due to the effects of Windows settings and the hardware
configuration.
The display language (English or Japanese) can be selected when the software is installed.
Windows 7 and Windows XP are registered trademarks of Microsoft Corporation
in the United States and other countries.
p
Microchip Electrophoresis System for DNA/RNA Analysis
11
MultiNA
MCE®-202 MultiNA is not available in the United States.
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its
affiliates, whether or not they are used with trademark symbol “TM” or “®”.
Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu
disclaims any proprietary interest in trademarks and trade names other than its own.
For Research Use Only. Not for use in diagnostic procedures.
The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu
does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
www.shimadzu.com/an/
© Shimadzu Corporation, 2012
Printed in Japan 3295-02203-30ANS
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertising