UV-3600 Plus UV-VIS-NIR Spectrophotometer C101-E128

UV-3600 Plus UV-VIS-NIR Spectrophotometer C101-E128
C101-E128
UV-VIS-NIR Spectrophotometer
UV-3600 Plus
Incorporating the latest technology to achieve
high sensitivity, high resolution, and an ultra-low
stray light level, leading the way to new solutions
UV-VIS-NIR Spectrophotometer
UV-3600 Plus
High Sensitivity
Shimadzu developed the world’s first UV-VIS-NIR spectrophotometer with three detectors, consisting of a PMT (photomultiplier
tube) for the ultraviolet and visible regions and InGaAs and cooled PbS detectors for the near-infrared region. With conventional
instruments that use only PMT and PbS detectors, there is a drop in sensitivity in the crossover region between those detectors.
Using an InGaAs detector to cover this crossover region, however, ensures high sensitivity across the entire measured wavelength
range, and gives a noise level of 0.00003 Abs at 1500 nm.
In addition to the main UV-3600 Plus unit, the Multi-purpose large-sample compartment and the Integrating sphere attachment
also feature three detector, enabling the high-sensitivity measurement of solid samples.
High Resolution, Ultra-Low Stray-Light, and Wide Wavelength Range
A high-performance double monochromator makes it possible to attain an ultra-low stray-light level (0.00005 % max. at 340 nm)
with a high resolution (maximum resolution: 0.1 nm). The wide wavelength range of 185 to 3,300 nm enables measurement
across the ultraviolet, visible, and near-infrared regions.
This instrument can perform spectrophotometry for a variety of applications.
The Advantages of Double-Grating Monochromators
The UV-3600 Plus uses gratings for both the pre-monochromator and main monochromator. Our holographic
diffraction gratings are designed to achieve high efficiencies and exceptionally low stray-light levels. Furthermore, to
improve light energy efficiency, blazed gratings are used for wavelength regions that by nature have low light source
energy or lower detector sensitivity. A G-G system that uses gratings for both the pre-monochromator and main
monochromator ensures low stray light, with a constant optimal resolution of 0.1 nm in the ultraviolet and visible
regions and 0.4 nm in the near-infrared region.
Wide Range of Optional Accessories
The three-detector Multi-purpose large-sample compartment or Integrating sphere options enable high-sensitivity measurement of
solid samples. Use the ASR Series of absolute specular reflectance accessories to perform high-accuracy absolute reflectance
measurements. In addition, temperature-controlled cell holders and micro cell holders are available to handle a wide range of
measurement applications.
MPC-603 Multi-purpose large-sample compartment
H igh S en sitiv it y
The UV-3600 Plus provides precise transmittance or reflectance measurements in the ultraviolet to near-infrared regions. The level of sensitivity in
the near-infrared region is significantly enhanced by using the combination of an InGaAs detector and a cooled PbS detector for this region.
Spectra can be obtained without interruption for the entire range, with a high level of sensitivity and precision.
InGaAs
PbS
InGaAs detector
PbS detector
PMT
Optical system around detectors
Sensitivity Characteristic
100
Relative Value (%)
PMT detector
PMT
InGaAs
PbS
Some spectrophotometers use a PMT (photomultiplier tube) for the ultraviolet and visible
region and a PbS detector for the near-infrared region. Neither detector, however, is very
0
1000
2000
sensitive near the detector-switchover region. This prevents high-sensitivity measurement in this
3000
range. The UV-3600 Plus makes it possible to take high-sensitivity measurements in the
Wavelength (nm)
switchover range by using an InGaAs detector.
Relationship between Detectors and Measurable Range
165nm
380nm
UV
PMT
InGaAs
PbS
780nm
Visible
3300nm
NIR
165 to 1000 nm
700 to 1800 nm
1600 to 3300 nm
Switching between the photomultiplier tube and the InGaAs detector is possible in the range of 700 to 1,000 nm (the default switchover wavelength is 830 nm).
Switching between the InGaAs detector and the PbS detector is possible in the range of 1,600 to 1,800 nm (the default switchover wavelength is 1,650 nm).
4
Comparison between Two-Detector and Three-Detector Measurements
With the UV-3600 Plus, an InGaAs detector is used in addition to a PMT (photomultiplier tube) and a cooled PbS detector. This serves to significantly reduce
the noise level in the InGaAs detector range (900 to 1,600 nm), in comparison to a conventional two-detector instrument (i.e., equipped with only PMT and a
PbS detectors).
0.300
UV-3600 Plus(InGaAs detector)
PbS detector
T%
0.200
0.100
The figure on the right shows transmittance spectra for water
measured with the UV-3600 Plus (InGaAs detector and cooled
0.000
PbS detector) and a conventional instrument (PbS detector) in the
range of 1,370 to 1,600 nm. The noise level is significantly less
-0.050
1370.0
with the UV-3600 Plus. (A mesh filter is used on the reference-
1400.0
1450.0
1500.0
beam side to maintain balance with the sample-beam side.)
1550.0
1600.0
nm
High-Accuracy Measurement with Minimized Detector Switchover Noise and Bump
Noise and bump caused by switching detectors is minimized to assure accurate measurement. Noise or bump is hardly observed even when using a transmission
cell with a long optical path of 50 or 100 mm.
0.400
1.500
0.300
Abs.
Abs.
1.00
0.200
0.500
0.100
0.000
700.0
800.0
900.0
nm
1000.0
0.000
1550.0
1600.0
1650.0
1700.0
nm
The figures above on the left and right are, respectively, transmittance spectra for ethylbenzene (obtained using a cell with an optical path of 100 mm) and cyclohexane (obtained using
a cell with an optical path of 10 mm). No baseline change is observed at the detector changeover wavelengths (870 and 1,650 nm).
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
5
High Resolution, Low Stray-Light Level,
and Wide Wavelength Range
The UV-3600 Plus is equipped with a high-performance, grating-grating double monochromator, and achieves a low stray-light level with
high resolution. The wavelength range is 185 to 3,300 nm. This instrument can perform spectrophotometry for various types of samples,
ranging from those requiring high resolution, such as gas samples, to highly concentrated liquid samples.
High-Resolution Spectra of Benzene Gas
The spectrum shown on the left was obtained by enclosing benzene gas in a cell with an optical-path length of 10 mm and performing measurement.
The spectral bandwidth is 0.1 nm. The triplet in the neighborhood of 250 nm (enlarged on the right) can be clearly observed. This instrument allows
high-resolution spectra to be measured with little noise.
1.800
0.350
1.500
0.300
Abs.
Abs.
0.250
1.000
0.200
0.500
0.150
0.100
249.0
0.000
240.0
245.0
250.0
255.0
249.5
250.0
250.5
251.0
nm
260.0
nm
Ultra-Low Stray-Light Level of 0.00005 % Max. (340 nm)
The figure below on the left is a spectrum for aqueous NaNO2 solution, and the figure on the right shows an enlarged view of the neighborhood of 340 nm.
In the figure on the right, the red spectrum is for aqueous NaNO2 solution and the blue spectrum is the 0 % line obtained when a shutter block is inserted on
the sample-beam side. The UV-3600 Plus achieves an ultra-low stray-light level of less than 0.00005 % at 340 nm.
(A mesh filter is used on the reference-beam side to maintain balance with the sample-beam side.)
100.000
0.00010
800.00
0.00005
T%
T%
60.00
40.00
0.00000
20.00
0.100
330.0
340.0
350.0
360.0
370.0
380.0
-0.00005
330.0
390.0
335.0
340.0
nm
345.0
350.0
nm
Linearity up to Absorbance Level 6
The figure on the left shows spectra obtained by measuring aqueous KMnO4 solution at six concentration levels. A mesh filter was inserted on the
reference-beam side and a differential method was used to perform measurement up to absorbance level 6. Using negative absorbance enables measurement
with little noise, even at high absorbance levels. The figure on the right shows the calibration curve using peak absorbances in vicinity of 525nm for aqueous
KMnO4 solution, and shows that linearity is maintained up to absorbance level 6.
6.000
Calibration curve
6..000
4.000
Abs.
Abs.
4..000
2..000
2.000
0.000
250.0
0..000
0..000
300.0
400.0
500.0
nm
6
600.0
700.0
100.000
200.000
300.000
Concentration (mg/L)
400.000
Covers Wide Wavelength Range from Ultraviolet to Near-Infrared
The wavelength range of 185 to 3,300 nm enables measurement over the ultraviolet, visible, and near-infrared regions.
In addition, the acquired spectra exhibit little noise across the entire range.
The figure on the right shows a spectrum obtained by measuring toluene in the range of 185 to 3,300
nm using a cell with an optical-path length of 2 mm.
100.000
Spectra in the ultraviolet, visible, and near-infrared regions can be obtained.
80.000
T%
60.000
40.000
20.000
0.000
185.0
1000.0
2000.0
3000.0
3300.0
nm
The figure on the right shows the spectrum for a low-transmittance film on a silica wafer in the range
of 200 to 1,600 nm. Although the film is a special type of film with a transmittance of almost zero, it
0.150
has been measured with high precision and little noise. (A mesh filter is used on the reference-beam
side to maintain balance with the sample-beam side.)
T%
0.100
0.050
0.000
-0.010
200.0
500.0
1000.0
1500.0 1600.0
nm
Molecules of alcohol such as 1-butanol are thought to consist of a mixture of non-hydrogen-bonded
isolates and aggregates formed through relatively weak hydrogen-bonding between OH groups.
2.500
As the temperature rises, the hydrogen-bonding becomes weaker and the aggregates separate into
isolates.
2.000
20℃
40℃
60℃
The figure on the right shows near-infrared spectra for 1-butanol obtained at 20 °C, 40 °C, and 60 °C.
The peaks in the neighborhood of 1,400 nm that become larger as the temperature increases are OH
smaller as the temperature increases are OH peaks for a hydrogen-bonded aggregate.
Abs.
1.500
peaks for a non-hydrogen-bonded isolate. The peaks in the neighborhood of 1,600 nm that become
1.000
Isolate-OH
0.500
Aggregate-OH
0.000
1300.0
1400.0
1500.0
1600.0
1700.0
1800.0
nm
As with the main UV-3600 Plus unit, the multi-purpose large-sample compartment and integrating
sphere attachment are also equipped with three detectors.
The figure on the right shows the near-infrared spectrum of a Si wafer that was measured with an
ISR-603 integrating sphere attachment (InGaAs detector + cooled PbS detector). Even solid samples
ISR-603 : InGaAs
PMT ISR-3100 : PbS
can be measured with low noise and high sensitivity, compared to measurements performed using
ISR-603 : InGaAs
PMT ISR-3100 : PbS
T%
T%
only a conventional PbS detector.
Red : ISR-603
Violet : ISR-3100
Blue : ISR-603
Violet : ISR-3100
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
7
A Wealth of Applications
Electrical, Electronics, and Optics
High-absorbance measurement of polarizing films
Reflection measurement of multilayer films
Absolute reflectance measurement of highly reflective mirrors
Spectral characteristic measurement of beam splitters
Relative emission measurement of LEDs
Transmittance measurement of quartz plates
Absolute reflectance measurement of anti-reflection coatings
Transmittance measurement of functional films
Diffuse reflectance measurement and band gap measurement of semiconductor materials
Transmittance measurement of solar cell cover glass, etc.
Food
Diffuse reflectance measurement of wheat flour
Quantitation of vitamins, food additives, and minerals
Quantitation of phenol elution in containers and packaging materials
Construction
Transmittance measurement of window glass and window glass films
Reflectance measurement of paints and building materials
Chemicals
Transmittance and color measurements of plastic materials
Reflectance measurement of silica-based white powered materials
Thickness measurement of thin films
Near-infrared measurement of organic solvents
Pharmaceuticals, Cosmetics, and Life Sciences
Cosmetic color measurement and ultraviolet screening measurement
Measurement of drugs containing crystallization water
Measurement of moisture in plants
Measurement of various amino acids
Quantitation of proteins and nucleic acids
Near-infrared measurement of pharmaceutical components
Textiles
Transmittance and reflectance measurements and ultraviolet screening measurement of textiles
Color measurement of textiles
8
Ele ctrica l, Elec tronic s, and Optic s
Transmittance Measurement of Very Small Samples
The current miniaturization of various products, such as sensors, means that the measurement of very small samples is now required. The figure shows the
results of a transmission spectrum measurement on a micro-sensor window.
When small samples are measured, the size of the light beam must be adjusted to the size of the sample. By using an optional MPC-603 unit and an aperture
unit (P/N 206-27362-91), the measurement beam can be narrowed to 2 mm in diameter, which allowsan appropriate optical system toube set up, even for
measurements of extremely small areas.
The MPC-603 is a UV-3600 Plus option that enables the measurement of various samples ranging from small large.
Ele ctrica l, Elec tronic s, and Optic s
Transmittance Measurement of Lenses
Imaging devices, such as mobile phones, digital cameras, and security cameras, are all equipped with lenses. The transmittance of a lens is one factor that
determines lens performance. However, because the lens itself focuses light, it is an especially difficult type of sample to measure accurately.
Because of the focal capability of lenses, the total light passing through the spectrophotometer during the measurement of the baseline can be different than
that after passing through the lens during measurement due to refraction. In such cases, the use of an integrating sphere can be used to collect all the light
passing through the lens and provide for more accurate measurements. In addition, using the transmission-type integrating sphere included with the BIS-603
can reduce measurement errors.
Furthermore, using the V stage, provided as standard on the MPC-603 multi-purpose large-sample compartment, allows measuring the transmittance of lenses
of various lengths and sizes.
The MPC-603 and BIS-603 are an ideal combination for measuring lenses.
Measurement of lens by
4-hole type of integrating sphere
Measurement of lens by
transmission-type integrating sphere
reference
lens
There may be measurement error caused by
reflectance differences between the reference
and internal surface of integrating sphere.
lens
There may no measurement error because
of same reflectance in internal surface
of integrating sphere.
Options Used for Measurements
MPC-603 Multi-Purpose Large-Sample Compartment
BIS-603 Sample Base Plate Integrating Sphere Set
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
9
A Wealth of Applications
Ele ctrica l, Elec tronic s, and Optic s
Absolute Reflectance Measurement of Mirrors
The reflectance of mirrors used in telescopes, lasers, and some other devices is extremely important because it serves as a factor in determining their
performance. The total light reflecting off of a sample can be composed of a specular component and a diffuse component. For mirrors, the specular
reflectance is the reflectance component of significance. In addition, reflectance measurements can be made as relative to the reflectance of a background
material or as absolute. Absolute values of specular reflectance are determined by use of an absolute reflectance measurement (ASR) accessory. The figure
below shows the results of measuring a mirror.
An absolute reflectance attachment, which enables measuring the reflectance of mirrors, can be installed on the optional MPC-603 unit.
Options Used for Measurements
MPC-603 Multi-Purpose Large-Sample
Compartment
ASR-3105/3112/3130/3145 Absolute Reflectance Attachment
Ref
100% light M2
Measurement light
M2'
Polarizer position
M3
M1
Integrating
sphere
Sample
Construction
BIS-603 Sample Base Plate Integrating Sphere Set
10
Large Polarizer Set, Polarizer Type I, Type II, Type III
E le ctrica l, Elec tronic s, and Optic s
Band Gap Calculation
Research into solar-cell and photocatalytic materials often involves the measurement of the band gap, which is a basic physical property of the materials.
Shown below are the diffuse reflectance spectra of three semiconductor materials used in the production of solar cells using the ISR-603 integrating sphere.
The absorption edge, the wavelength where the reflectance decreases, differs depending upon the sample type. This difference indicates a difference in the
band gap* of the samples.
The band gaps of the samples were calculated using the Tauc method, and were determined to be 1.63ev for GuGaSe2 (red line) 1.27 eV for Culn0.5Ga0.5Se2
(blue line) and 0.99 eV for CuInSe2 (black line).
* The band gap refers to the energy difference between the top of the valence band filled with electrons and the bottom of the conduction band devoid of electrons.
The wavelength range of the UV-3600 Plus is extremely effective for band gap calculations.
* The samples are courtesy of the Wada Laboratory, Faculty Science and Technology, Ryukoku University.
Sample
Band Gap Value
CuGaSe 2
1.63 eV(757nm)
Culn 0.5Ga 0.5Se 2
1.27eV (977nm)
CuInSe 2
0.99eV(1253nm)
Options Used for Measurements
Excel Macro for Band Gap Calculation
ISR-603 Integrating Sphere Attachment
300
2
(hν
(R∞)
)
250
200
150
100
This hγ value indicates
the band gap value.
50
0
0.7 0.8 0.9 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2
hν
(eV)
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
11
A Wealth of Applications
C h emicals
Thickness Measurement of Plastic Wrap for
Food Packaging
Interference wave patterns sometimes occur when light passes through a thin,
transparent film. The film thickness of a sample can be determined from the
wavelength intervals of these interference wave patterns. The figure shows the
transmittance spectrum of plastic wrap for food packaging. An interference
wave pattern can be seen. By utilizing the optional film thickness measurement
software, the film thickness was calculated to be 10.4 μm.
Note: The refractive index of the sample must be entered in order to perform the
calculation.
The number of interference fringes decrease as the film gets thinner. The wide
wavelength range of the UV-3600Plus allows for the measurement of
interference fringes for very thin films.
Options Used for Measurements
Film Thickness Measurement Software
12
Film Holder
Textile s
Diffuse Reflectance Measurement of
Various Fabrics
The diffuse reflectance spectra of various textiles were measured in the visible
region using the ISR-603 integrating sphere attachment.
The blue line indicates a blue fabric and the red line indicates a red fabric. The
blue fabric mainly reflects short-wavelength blue light, so it appears blue, while
the red fabric mainly reflects long-wavelength red light, so it appears red.
The combination of the UV-3600 Plus and the ISR-603 is ideal for measuring
color. Also, the optional color measurement software enables colors to be
calculated and displayed in a variety of color space models.
Options Used for Measurements
Color Measurement Software
ø60mm ISR-603 Integrating Sphere Attachment
13
A Wealth of Applications
C o n stru ction
Transmission Measurement of Window Glass (Solar Transmittance)
In recent years, measures to offset the effects of Global Warming and Heat Islands have included the incorporation of various kinds of functional glass into
modern building materials. These new glass materials reduce the transmittance of infrared radiation and also offer thermal insulation potential.
Solar transmittance is defined by JIS as an index that represents the transmission characteristics of sunlight, from visible to near-infrared light. The figure below
shows the results of measuring the transmission spectra of various types of glass and calculating their solar reflectance. It reveals that solar transmittance
varies depending on the type of glass.
This method of measuring solar transmittance requires a 250 to 2100 nm measurement range and an integrating sphere. The combination of the UV-3600
Plus and ISR-603 is ideal for solar transmittance measurements.
Solar Transmittance (Te)
Visible Light Transmittance (TV)
Transparent glass 1
Data Name
81.412
88.733
Transparent glass 2
89.612
91.339
Heat-absorbing glass 1
47.428
75.051
Heat-absorbing glass 2
44.367
43.323
Heat-absorbing glass 3
52.023
50.029
Options Used for Measurements
ISR-603 Integrating Sphere Attachment
14
DTL-UVPC Solar Transmittance Measurement Software
P h arma ceutic als
Measurement of Anhydrous Caffeine in Cold Remedy
Anhydrous caffeine is included in cold medicines. The figure below shows the results of measuring anhydrous caffeine using a powdered sample holder.
It enables volumes as small as 0.16 mL to be measured easily.
The combination of the UV-3600 Plus, ISR-603, and powdered sample holder is ideal for the diffuse reflectance measurement of powder.
Options Used for Measurements
Powdered Sample Holder
ISR-603 Integrating
Sphere Attachment
Fo o d
Evaluation of Light-Shielding Effect of Food Containers
Foods on display in shops are exposed to various types of light, including ultraviolet, visible, and near-infrared light. Such exposure can cause protein
oxidation, vitamin destruction, fading, and other adverse effects on the foods. Therefore, it’s important to examine the light-shielding effect (transmittance)
of food containers. In this example, the left figure shows the transmittance spectra of a PET bottle and a paper-based milk carton measured by the
UV-3600 Plus and MPC-603. The figure on the right shows the ultraviolet region from 200 to 380 nm. The light shielding effect, especially in the UV
region, of the paper-based container is superior to that of the PET bottle.
100.0
T%
100.0
T%
PET bottle
PET bottle
80.0
80.0
60.0
60.0
40.0
40.0
20.0
20.0
Milk carton
Milk carton
0.0
200.0
500.0
1000.0
1500.0
2000.0 2300.0
nm
0.0
200.0
250.0
300.0
350.0
380.0
nm
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
15
UV Pro b e S o f t w a r e
All-In-One Software
UVProbe is an all-in-one software package equipped with the following four
functions:
Spectrum Module
Spectrum module
Photometric module (quantitation)
Kinetics module (time-course measurement)
Report generator
Each can be easily operated from its own special screen. In addition to a
wealth of data processing functions, including peak detection and area
calculations, the software is equipped with security functions to configure
operational authority user by user, as well as data audit trail and equipment
audit trail functionality.
Photometric Module
Kinetics Module
Report Generator
The report generator provides the freedom to arrange graphs, tables, etc. to suit users' needs.
The thickness and color of graph lines, as well as font size, can now be specified. Pasting labels on
graphs and editing text is as easy as can be, allowing the user to effectively print comments along
with the analysis results.
16
Optio n al S o f t w a r e
DTL-UVPC Solar Transmittance Measurement Software
(P/N 206-23130-91)
This software calculates the solar transmittance, solar reflectance, visible-light
transmittance,and visible-light reflectance of sheet glass and films. It can also calculate some
color-relatedproperties (tristimulus values, chromaticity coordinates, dominant wavelength,
and excitation purity).
• Create and save custom weighting coefficient tables as files.
• Recalculate existing data after changing standard samples.
• Create custom illumination for color calculations, and save the created illumination as a file.
• Perform white plate correction for highly accurate calculations, and save the white plate reflectance data as a file.
• Graph chromaticity coordinates.
Note: This software runs on Windows 7 Professional.
Color Measurement Software
Measurement window
(P/N 206-67449)
This software calculates color values of the measurement sample from the spectrum measured.
• Calculable Items
Tristimulus value (XYZ), chromaticity coordinates (xy), Hunter color coordinate system/color difference formula,
CIELAB color coordinate system/color difference formula, CIELUB color coordinate system/color difference formula,
yellowness/after-yellowing, whiteness, whiteness B (blue reflectance), Munsell, metamerism, three attributes from
CIELUB and their difference, primary wavelength, excitation purity
• The software is fully equipped with convenient graphic functions including chromaticity diagrams and enlarged
color-difference views.
• It provides a wealth of recalculation functions, enabling items and conditions with respect to the spectra obtained to
be changed for recalculation.
• The visual field (2°, 10°) and the illumination (A, B, C, D65, F6, F8, and F10) are freely selectable. In addition, the user
can configure particular weighting coefficients, enabling calculations with respect to any illumination. The configured
illumination can also be saved.
• Standard white plate values can be configured, enabling corrected calculations.
• Standard samples can be freely specified, enabling color-difference calculations.
• Thickness conversion calculations are possible with respect to glass, filters, and other transmissive materials.
• The average and standard deviation of multiple data points can be calculated.
• Up to 100 data points can be shown.
Note: This software runs on Windows 7 Professional.
Lab chromaticity diagram display window
Film Thickness Measurement Software
(P/N 206-66877)
This software measures the thickness of thin films from the wavelengths of peak (or valley)
interference waveforms overlapping the spectrum.
The film thickness is measured through optical methods without physical contact.
• The film thickness is calculated from linear regression by applying the method of least squares to the wavelengths
of the multiple peaks and valleys automatically detected. (The thin film's refractive index and the angle of
incidence must be configured as calculation conditions.)
• The calculation conditions can be changed with respect to the measured spectra, enabling recalculation.
• A range can be set for use in the calculations while checking the spectral interference waveform onscreen.
• The measurable film thickness range is (minimum measured wavelength)/n to 50 × (maximum measured
wavelength)/n. (Reference value)
Note: This software runs on Windows 7 Professional.
Recalculation window
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
17
Accessories
ISR-603 Integrating Sphere Attachment
(P/N 207-20100-58)
This attachment is used for the measurement of diffuse and spealar
reflectance. Transmission of liquid and solid samples can also be measured.
●
Wavelength range
: 220 to 2600 nm
●
Integrating sphere
: 60 mm I.D.
Mirror M3
Reference beam
Mirror M2
BaSO4
●
Detectors
●
Maximum size of reflective sample : Approx. 100 mm dia. × 15 mm thick
: PMT, InGaAs, cooled PbS
●
Incident angle
90°
8°
: 0 deg. / 8 deg.
Note: The optional cuvette attachment is required for transmission measurement of liquid samples.
Sample beam
ø60 integrating
sphere
Mirror M1
Specular Reflectance Measurement Attachment (5° Incident Angle)
Structure
(P/N 206-14046)
Sample
(measuring surface down)
The technique of specular reflectance measurement is often applied to the
examination of semiconductors, optical materials, multiple coating/firm layers, etc,
and are relative to a reference reflecting surface. The 5° incident angle minimizes the
influence of polarized light so no polarizer is required, providing for a simple
operation.
●
Optics
Samples as large as W100 × D160 × T15 mm can be readily measured. The minimum size is 7 mm in
diameter.
Light beam
• Sample placement is easy - just set it on a holder with the measuring surface down.
Powdered Sample Holder
(P/N 206-89065-41)
This powdered sample holder simplifies the reflectance measurement of powder
samples using an integrating sphere. The container is ideal for powders that do not
pack well into conventional powder holders. It can be attached to all Shimadzu
integrating spheres.
●
Capacity of 0.16 mL, 3 included
MPC-603 Multi-Purpose Large-Sample Compartment
(P/N 207-20600-58)
This multi-purpose sample compartment allows measurement of various shaped
samples to obtain both reflection and transmission spectra. An integrating sphere is
built-in to ensure accurate measurement of solid samples.
:PMT, InGaAs, cooled PbS
• Maximum sample size :Transmission 305 mm dia. × 50 mm thick
or 204 mm dia. × 300 mm thick
Reflection 305 mm dia. × 50 mm thick
• Original S-beam/R-beam switching function allows reflection measurement using incidence angles
of 0 deg. for diffuse reflectance measurements and 8 deg. without leaning the sample for total
1020
Note 1: A separate space must be provided for the
personal computer.
Note 2: A small table for the MPC-603 must be
provided separate from that for the UV-3600 Plus.
Table dimensions: Able to accommodate MPC-603
unit (W580 × D420 mm)
Table height: Within +8 to -15 mm of the UV-3600
Plus table height
UV-3600 Plus
660
:240 to 2600 nm
• Detectors
MPC-603
reflectance measurements.
• The V stage is built-in. The sample position can be freely adjusted both forward and backward as well as up and down.
18
581
436
• Wavelength range
Unit: mm
Absolute Reflectance Attachment
Ref
100% light M2
Model
P/N
ASR-3105 Absolute Reflectance Attachment, 5°
206-16817
Measurement light
M2'
Polarizer position
M3
ASR-3112 Absolute Reflectance Attachment, 12°
206-16100
ASR-3130 Absolute Reflectance Attachment, 30°
206-15001
ASR-3145 Absolute Reflectance Attachment, 45°
206-15002
M1
Integrating
sphere
Sample
These accessories are intended for use with the MPC-603 Sample Compartment and
require the BIS-603 Sample Base Plate-Integrating Sphere Set (P/N 206-17059). At larger
angles of incidence (30°, 45°), a polarizer is also required.
• Wavelength range
: 300 to 800 nm
• Accuracy
: with respect to 90 % reflectance samples
• Incidence angle 5°
: ±1.5 %
Incidence angle 12°
: ±1.0 %
Construction
• 100 % level sample setting : The sample measurement optical path can be
switched using the single-touch V-N method.
• Approximate sample size : 25 to 200 mm dia., or 20 to 150 mm square, up to 30
mm thick
Incidence angle 30°, 45° : ±2.5 %
Note: The BIS-603 Sample Base Plate-Integrating Sphere Set (P/N 207-21100-58) is required for mounting these absolute specular reflectance attachments.
BIS-603 Sample Base Plate Integrating Sphere Set
(P/N 207-21100-58)
This base plate is used to install an absolute specular reflectance attachment in the MPC-603
multi-purpose chamber.
ASR-3112 attached on the BIS-603
Large Polarizer Set, Polarizer Type I, Type II, Type III, Polarizer Adaptor Set
Polarizers are needed to obtain highly precise absolute reflectance measurements at large incident angles
without affecting the polarization characteristics.
The Polarizer Adaptor set (P/N 206-15693) is required for the Polarizer Type I, Type II and Type III.
Model
Large Polarizer Set
P/N
Effective Diameter
Wavelength Ranges
206-15694
20mm
250 to 2500 nm
Polarizer Type I
206-13236-01
18mm
400 to 800 nm
Polarizer Type II
206-13236-02
17mm
260 to 700 nm
Polarizer Type III
206-13163
10mm
260 to 2500 nm
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
19
A ccess o r ies
Cells
Optical Path (L) Required Sample Volume
Description
10 mm
Type
Fused Silica (S)
①
2.5 to 4.0 mL
20 mm
5.0 to 8.0 mL
50 mm
12.5 to 20.0 mL
100 mm
25.0 to 40.0 mL
Square cell with stopper
10 mm
2.5 to 4.0 mL
②
Semi-micro cell
10 mm
1.0 to 1.6 mL
③
*1
Semi-micro black cell
10 mm
1.0 to 1.6 mL
③’
*1
5 mm
25 to 100 μL
⑦’
*2
10 mm
50 to 200 μL
⑦
*2
10 mm
50 to 400 μL
⑧
*2
10 mm
3.8 mL
Rectangular cell
Supermicro black cell
Micro black cell
Cylindrical cell
Short path cell
20 mm
7.6 mL
50 mm
19.0 mL
100 mm
38.0 mL
1 mm
0.3 to 0.4 mL
2 mm
0.5 to 0.8 mL
5 mm
1.3 to 2.0 mL
4
⑥
④
⑤
Glass (G)
Fused Silica (IR)
200-34442
200-34565
200-66579-01
200-34446
200-34446-01
200-66579-02
200-34944
200-34944-01
200-66579-03
200-34676
200-34676-01
200-66579-04
200-34444
200-34444-01
200-66579-21
200-66501
200-66501-01
200-66579-11
200-66551
—
208-92116
—
—
200-66578-11
—
—
200-66578-12
—
200-66579-12
—
200-34448
(silica window)
200-34448-01 (glass window)
200-66579-31 (silica window IR)
200-34472
(
)
200-34472-01 (
)
200-66579-32 (
)
200-34473-01 (
)
200-34473-03 (
)
200-66579-33 (
)
200-34473-02 (
)
200-34473-04 (
)
200-66579-34 (
)
200-34660-01
200-34662-01
200-66579-05
200-34655
200-34662-11
200-66579-06
200-34449
200-34449-01
200-66579-07
4
12.5
25
45
12
25ø
3
12
.5
①
②
③
12.5
③’
12
12
2
④
⑤
⑥
⑦
.5
2
2
⑦’
⑧
Unit: mm
Note *1 With a 5 mm slit, the cell holder with micro cell mask (P/N 204-06896) is required.
*2 The supermicro cell holder (P/N 206-14334) is required.
Film Holder
Rotating Film Holder
(P/N 204-58909)
(P/N 206-28500-41)
Used in transmittance measurement of thin samples such as films
and filters.
This film holder can rotate samples in a plane centered on the optical axis.
Polarizers Type I, II, and III can be attached to the base plate. The Large
Polarizer Set cannot be used.
• Sample size : 33 mm × 30 mm × 2 mm thick
• Sample Size
Minimum : W16 × H32 mm
Maximum : W80 × H40 × t20 mm
Four-Cell Sample Compartment Unit
Multi-Cell Sample Compartment
(P/N 206-23670-91)
(P/N 206-69160-01)
Accommodates 4-cell holders of various types.
Holds up to six 10-mm square cells on the sample side. No temperature
control capability.
• Incorporates a 4-cell holder
for 10-mm standard cells.
• Number of cells : 6 on the sample side
1 on the reference side
Note: Cells are not included.
20
Universal Rectangular Cell Holder,
Four-Cell Type (P/N 204-27208)
Reference-Side Rectangular Long-Path
Absorption Cell Holder (P/N 204-28720)
Holds four rectangular cells with an
optical path length of 10, 20, 30, 50,
70, or 100 mm.
If using a 4-cell-type universal rectangular cell holder, only use a
reference-side cell holder if necessary.
Note:
The Four-Cell Sample Compartment unit (P/N
206-23670-91) is required. When a rectangular,
long-path cell is used on the reference side, its
holder (P/N 204-28720) is required.
Long-Path Rectangular Cell Holder
Cylindrical Cell Holder
(P/N 204-23118-01)
(P/N 204-06216-02)
Holds two rectangular cells with
an optical path length of 10, 20,
30, 50, 70, or 100 mm.
Holds two cylindrical cells with
an optical path length of 10,
20, 50, or 100 mm.
Super Micro Cell Holder
Micro Cell Holder with Mask
(P/N 206-14334)
(P/N 204-06896)
Holds supermicro cells for
measurement of extremely small
volume samples. The cell height is
adjustable, and the required
sample volume can be adjusted in
the range of 50 to 200 μL, depending on
the type of black cell used.
Required when using semi-micro
cells or micro cells with an
optical path width of 3 mm or
less. (The mask width can be
adjusted.)
• Applicable cells : ⑦, ⑦', and ⑧ in the list of cells on page 12.
Cells are not included.
• Mask : Choice of W1.5 × H1 mm or W1.5 × H3 mm
3-μL Capillary Cell Set for Ultramicro Volume Measurement
(P/N 206-69746)
Recommended for small-volume and precious samples, such as in biological applications. Solution
sample is drawn into the capillary cell by capillary action. The end of the capillary cell is sealed with
the critoseal and the capillary cell is set in the capillary cell adapter, where it is analyzed. The holder is
the same size as a 10-mm standard cell and can be mounted to the standard cell holder.
• The minimum sample volume required : 3 μL when tube closure is used (theoretical value)
• Supplied with 100 capillaries (made of quartz) and a tube closure
• Inner diameter of capillary : 0.5 mm
Note: Usually, the effective optical path length is approximately one-twentieth of a 10-mm square cell.
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
21
Acce ss o ries
8/16-Series Micro Multi-Cell
Cell Holders
Model
P/N
8/16 Series Micro Multi-cell Holder MMC-1600
206-23680-91
8/16 Series Constant-Temperature Micro Multi-cell Holder MMC-1600C
206-23690-91
This cell holder holds one 8 or 16-cell micro multi-cell for micro-volume
measurement. Two types of micro multi-cell holders are available: the standard type
(MMC-1600) and the constant-temperature water circulation type (MMC-1600C).
• Applicable temperature range : 10 °C to 60 °C (C type)
• Temperature deviation between circular water and cell : max. 3 °C (C type)
• Temperature stabilizing time : 15 min or less (C type)
Micro Multi-Cells
Model
P/N
8-series Micro Multi-cell; optical path length; 10 mm; cell volume: 100 μL
208-92089
16-series Micro Multi-cell; optical path length: 10 mm; cell volume: 100 μL
208-92088
8-series Micro Multi-cell; optical path length: 5 mm; cell volume: 50 μL
208-92086
16-series Micro Multi-cell; optical path length: 5 mm; cell volume: 50 μL
208-92085
There are two types of micro multi-cells available in both the 8-series and 16-series
models: a 50 μL type and a 100 μL type. The cell intervals of the 8-series micro
multi-cells are applicable for use with 8 × 12-well microplates and 8-channel
pipettes. Microplate samples aspirated into multi-channel pipettes can be injected
directly into the cells for measurement.
• Micro-volume samples can be measured. (Minimum sample
volume : 50 μL to 100 μL)
• Support for commercial microplates and micro pipettes (with
8-series micro cells).
• Up to 16 samples can be measured at a time (with 16-series
micro cell).
CPS-100 Cell Positioner, Thermoelectrically Temperature Controlled
(P/N 206-29500-41)
This attachment permits measurement of up to six sample cells under
constant-temperature conditions. Combination of this attachment and the Kinetics
mode of UVProbe software provides measurement of temperature-sensitive enzyme
kinetics of one to six samples.
• Number of cells : 6 on the sample side (temperature-controlled)
1 on the reference side (temperature not controlled)
• Temperature control range : 16 °C to 60 °C
• Temperature display accuracy (difference from the true value) : ±0.5 °C
• Temperature control precision (variation of temperature) : ±0.1 °C
• Ambient temperature : 15 °C to 35 °C
Note: Standard cells (P/N 200-34442) is not included, please purchase separately.
A USB adapter CPS (P/N 206-25234-91) is required.
TCC-100 Thermoelectrically Temperature Controlled Cell Holder
(P/N 206-29510-41)
Uses the Peltier effect for controlling the sample and reference that meets the
following specifications :
no thermostated bath or cooling water is required.
• Number of cells : One each on the sample and reference sides (temperature-controlled)
• Temperature control range : 7 °C to 60 °C
• Temperature display accuracy (difference from the true value) : ±0.5 °C
• Temperature control precision (variation of temperature) : ±0.1 °C
Note: Standard cells (P/N 200-34442) is not included, please purchase separately.
22
Constant-Temperature Cell Holder
(P/N 202-30858-04)
Maintains a sample cell and reference cell at a desired, uniform temperature by circulating
constant-temperature water.
• Temperature range : 5 °C to 90 °C
(depends on the performance of the constant-temperature water circulator)
• Cell holder : Accepts a pair of 10-mm standard cells.
• Connecting joint outer diameter : 6 mm and 9 mm (two levels)
Constant-Temperature Four-Cell Holder
(P/N 204-27206-02)
Maintains four sample cells and a reference cell at a desired, uniform temperature by
circulating constant-temperature water.
• Temperature range : 5 °C to 90 °C
(depends on the performance of the constant-temperature water circulator)
• Cell holder : Accepts four 10-mm standard cells plus a reference cell
• Connecting joint outer diameter : 9 mm
Note: The Four-Cell Sample Compartment Unit (P/N 206-23670-91) is necessary.
S-1700 Thermoelectric Single-Cell Holder
(P/N 206-23900-91)
This cell holder permits setting of a temperature program to increase and decrease the sample cell temperature.
• The thermoelectric system allows prompt control of sample temperature between 0 °C and 110 °C.
• Temperature increase/decrease speed can be changed using 12 settings, which means the holder can be used in analysis
of melting curves for nucleic acids, etc. that occur during quick as well as slow heating (or cooling).
• A stirrer is also provided to ensure uniform temperature distribution throughout the cell.
• Cooling water circulation is required for Peltier element cooling. And
though tap water can be used, it is recommended that a commercially
• Cells are not supplied. Please use 10-mm
available constant-temperature water circulator be used, as the
standard tight-sealing cells (a Hellma product).
following conditions must be fulfilled to exact maximum performance
• Temperature accuracy in cell
from the S-1700.
(when room temperature is 25 °C) :
Cooling water specification : 20 ± 2 °C
Water flow : 4.8 L/min or more.
• Temperature is not controlled at the reference side.
Type
Minimum Sample
Volume Required
Optical Path Length
110-QS-10
10 mm
3.5 mL
115B-QS-10
10 mm
400 μL
Within 0.25 °C (0 °C to 25 °C)
Within ±1 % of set value (25 °C to 75 °C)
Within ±2 % of set value (75 °C to 110 °C)
TMSPC-8 Tm Analysis System
(P/N 206-24350-91)
This system obtains a temperature-versus-absorbance curve data, and the Tm Analysis
Software analyzes the Tm (melting temperature) of nucleic acids such as DNA and RNA.
The system consists of an 8 Series Micro Multi-Cell Holder, Tm Analysis Software, and
Temperature Controller. 8 Series Micro Cells, Silicone Cap, and Constant-Temperature Water
Circulator for protecting the Peltier device are not included. Please purchase separately.
Model
P/N
8 Series Micro Cell Optical Path 10 mm, Sample Volume 100 μL
208-92097-11
8 Series Micro Cell Optical Path 1 mm, Sample Volume 35 μL
208-92140
Silicone Cap for Micro Cell (24 pcs)
206-57299-91
• Temperature control range : 0.0 to 10.0 °C
• Tm Calculation mode : Average Method, Differential Method
• OS : Windows 7 Professional
Note: Please purchase a constant-water circulator that meets the following specifications :
Temperature range : 20 ± 2 °C, Flow rate: 4.8 L/min or more
Inner diameter of the connecting pipe : 8, 10, 12 mm dia.
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
23
A ccess o ries
NTT-2200P Constant-Temperature Water Circulator
(P/N 208-97263)
Circulates temperature-controlled water to a constant-temperature cell holder.
• Temperature range
: Ambient +5 °C to 80 °C
• Temperature control precision : ±0.05 °C or more
• Maximum pumping rate
: 27/31 L/min, 9.5/13 m (50/60 Hz)
• External circulation nozzle
: 10.5 mm OD (both outlet and return)
• Tank capacity
: About 10 L (9 L during use)
• Safety features
: Detection of over-temperature of Upper or Lower limits,
Detection of heater wire malfunction, Protection from heating too little circulating water,
Detection of sensor malfunction, Independent over-heat protection, Over-current circuit protector
• Standard accessories
: Lid with handles, Rubber hose (4 m; inner diameter: 8 mm; outer diameter: 12 mm; quantity: 1),
• Dimensions
: W270 × H560 × D400 mm
• Power requirements
: 100 VAC, 1,250 VA, with 1.7-m power cord and grounded plug
Hose clamps (4 pcs), instruction manual (Japanese and English)
Sipper Unit
P/N
Standard Sample Volume
Sipper Unit 160L (Standard Sipper)
Model
206-23790-91
2.0 mL
Sipper Unit 160T (Triple-Pass Sipper)
206-23790-92
1.5 mL
Sipper Unit 160C (Constant-Temperature Sipper)
206-23790-93
2.5 mL
Sipper Unit 160U (Supermicro Sipper)
206-23790-94
0.5 mL
Four types of sipper units with different flow cell types are available.
The stepping motor-driven peristaltic pump ensures reliable and smooth
aspiration of sample solution.
Note:
The use of a Solenoid Valve (Fluoropolymer) (P/N 204-06599-01) and
the SWA-2 Sample Waste Unit (P/N 206-23820-91) are recommended
when measuring strong acids, strong alkalis, or organic solvents.
(These sippers can be controlled directly from the UV-3600 Plus so no interface is required.)
Syringe Sipper
Model
P/N
Syringe Sipper N (Normal temperature type)
206-23890-51
Syringe Sipper CN (Constant temperature, water circulator type)
206-23890-92
The sipper unit employs a syringe-pump system. The liquid-contact
surfaces are composed of a Fluoropolymer, glass, or quartz, imparting
excellent chemical resistance and ease of maintenance, and allowing
measurement of almost any sample type. Further, the extremely high
repeatability of the sipping volume (repeat precision: ±0.03 mL) makes it
ideal when performance validation is required.
Note: Flow cell available separately. Choose from the recommended flow cells listed below.
in accordance with the application.
• The flow cell can be changed
independently for excellent ease of
maintenance. (CN type)
• Circulated-water temperature range :
Recommended Flow Cells
24
• The type of flow cell can be selected
ambient to 60 °C
Cell Type
P/N
Optical Path Length
Dimensions of Aperture
Standard Required Sample Volume
Square (ultra-micro)
208-92114
10 mm
2 mm dia.
0.9 mL
Square (micro)
208-92113
10 mm
3 mm dia.
1.0 mL
mask R (P/N 206-88679) to the reference cell holder is
Square (semi-micro)
208-92005
10 mm
11 (H) × 3.5 (W) mm
5.0 mL
recommended to balance the light intensity.
Note: If a square flow cell (micro or supermicro) is used, attaching
ASC-5 Auto Sample Changer
(P/N 206-23810-91)
Combine with a Sipper 160 to build an automated multi-sample
spectrophotometry system.
• The aspirating nozzle is programmed to move in the X, Y, and Z (vertical) directions.
• Up to 8 sets of operational parameters, including rack size and the numbers of test
tubes, can be set. The numbers are stored in battery backed-up internal memory.
• Up to 100 test tubes may be set together on the rack.
Note 1: An ASC USB adapter (P/N 206-25235-91) is required.
Note 2: A commercially available test tube stand, with a footprint smaller than 220 × 220 mm, is applicable.
UV Automation System Connection Kit
(P/N 206-80880-01)
This kit makes it possible to connect the instrument to a Gilson GX-271 liquid handler.
The liquid handler automatesvarious pretreatmenttasks, such as dispensing and diluting
the sample and adding reagents.
This kit enables the spectrophotometer to operate together with the liquid handler
during measurements.
• The connection kit consists of a flow cell unit and connection cable.
Connection
cable
Flow cell unit
10-mm Micro Flow-thru Cell with Holder (P/N 204-06222)
5-mm Micro Flow-thru Cell with Holder (P/N 204-06222-01)
P/N
Optical Path Length
Volume
204-06222
10 mm
0.3 mL
204-06222-01
5 mm
0.15 mL
Used for the continuous analysis of samples such as the liquid output
of column chromatography.
• Inner diameter of tube : 1 or 2 mm
Front Panel with Holes
(P/N 204-27588-03)
Allows the tubes of a flow cell, for example, to be connected through the front panel
of the instrument.
Flow-Thru Cell for HPLC
(P/N 206-12852)
With this flow-thru cell attached, the spectrophotometer can be used as a
variable-wavelength UV-VIS detector for an HPLC system.
• Inner diameter : 1 mm; Optical path length : 10 mm; Inner volume : 8 μL
• Flow-thru cell on the sample side and cell holder with a mask on the reference side.
• SUS tube : Outer diameter : 1.6 mm; Inner diameter : 0.3 mm
UV-3600 Plus
UV-VIS-NIR Spectrophotometer
25
UV-3600 Plus
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www.shimadzu.com/an/
© Shimadzu Corporation, 2014
Printed in Japan 3655-12415-20AIT
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