TruSeq Rapid Enrichment Library Prep Protocol Guide (1000000000753 v00)

TruSeq Rapid Enrichment Library Prep Protocol Guide (1000000000753 v00)
TruSeq Rapid Exome Library Prep
Protocol Guide
For Research Use Only. Not for use in diagnostic procedures.
Tagment Genomic DNA
Clean Up Tagmented DNA
Amplify Tagmented DNA
Clean Up Amplified DNA
Hybridize Probes
Capture Hybridized Probes
Perform Second Hybridization
Perform Second Capture
Clean Up Captured Library
Amplify Enriched Library
Clean Up Amplified Enriched Library
Validate Enriched Libraries
Acronyms
Technical Assistance
ILLUMINA PROPRIETARY
Document # 1000000000753 v00
November 2015
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This document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"), and are intended solely for the
contractual use of its customer in connection with the use of the product(s) described herein and for no other purpose. This
document and its contents shall not be used or distributed for any other purpose and/or otherwise communicated, disclosed,
or reproduced in any way whatsoever without the prior written consent of Illumina. Illumina does not convey any license
under its patent, trademark, copyright, or common-law rights nor similar rights of any third parties by this document.
The instructions in this document must be strictly and explicitly followed by qualified and properly trained personnel in order
to ensure the proper and safe use of the product(s) described herein. All of the contents of this document must be fully read
and understood prior to using such product(s).
FAILURE TO COMPLETELY READ AND EXPLICITLY FOLLOW ALL OF THE INSTRUCTIONS CONTAINED HEREIN
MAY RESULT IN DAMAGE TO THE PRODUCT(S), INJURY TO PERSONS, INCLUDING TO USERS OR OTHERS, AND
DAMAGE TO OTHER PROPERTY.
ILLUMINA DOES NOT ASSUME ANY LIABILITY ARISING OUT OF THE IMPROPER USE OF THE PRODUCT(S)
DESCRIBED HEREIN (INCLUDING PARTS THEREOF OR SOFTWARE).
© 2015 Illumina, Inc. All rights reserved.
Illumina, 24sure, BaseSpace, BeadArray, BlueFish, BlueFuse, BlueGnome, cBot, CSPro, CytoChip, DesignStudio,
Epicentre, ForenSeq, Genetic Energy, GenomeStudio, GoldenGate, HiScan, HiSeq, HiSeq X, Infinium, iScan, iSelect,
MiSeq, MiSeqDx, MiSeq FGx, NeoPrep, NextBio, Nextera, NextSeq, Powered by Illumina, SureMDA, TruGenome,
TruSeq, TruSight, Understand Your Genome, UYG, VeraCode, verifi, VeriSeq, the pumpkin orange color, and the
streaming bases design are trademarks of Illumina, Inc. and/or its affiliate(s) in the U.S. and/or other countries. All other
names, logos, and other trademarks are the property of their respective owners.
Tagment Genomic DNA
Tagment Genomic DNA
Preparation
1
Save the following TAG58 program on the thermal cycler:
} Choose the preheat lid option and set to 100°C
} 58°C for 10 minutes
} Hold at 10°C
} Each well or tube contains 50 μl.
2
Save the following TAG60 program on the thermal cycler:
} Choose the preheat lid option and set to 100°C
} 60°C for 5 minutes
} Hold at 10°C
} Each well or tube contains 65 μl.
Procedure
1
Quantify gDNA using a fluorometric method.
2
Dilute gDNA in Tris-HCl 10 mM, pH 8.5 to a final volume of 10 μl at 5 ng/μl.
3
Add the following items in the order listed to a new Hard-Shell PCR plate or to a
new 0.2 ml thin-wall PCR tube or 8-tube strip.
} TD (25 μl)
} Normalized gDNA (10 μl)
} TDE2 (15 μl)
4
Mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
5
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
6
Place on the preprogrammed thermal cycler and run the TAG58 program.
7
Add 15 μl ST2, and then pipette to mix
8
Place on the preprogrammed thermal cycler and run the TAG60 program.
TruSeq Rapid Exome Library Prep Protocol Guide
3
Clean Up Tagmented DNA
Procedure
1
Transfer all supernatant to a new midi plate or to a new 1.5 ml microcentrifuge tube.
2
Add 52 μl SPB, and then mix thoroughly as follows.
} [Plate] Pipette up and down 10 times.
} [Tube] Pipette up and down.
3
Incubate at room temperature for 5 minutes.
4
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
5
Transfer 98 μl supernatant to a new midi plate or to a new 1.5 ml microcentrifuge
tube.
6
Add 137 μl SPB, and then mix thoroughly as follows.
} [Plate] Pipette up and down 10 times.
} [Tube] Pipette up and down.
7
Incubate at room temperature for 5 minutes.
8
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
9
Remove and discard all supernatant.
10 Wash 2 times with 200 μl 80% EtOH.
11 Using a 20 μl pipette, remove residual 80% EtOH.
12 Air-dry on the magnetic stand for 5 minutes.
13 Add 22.5 μl RSB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
14 Remove from the magnetic stand.
15 Incubate at room temperature for 2 minutes.
16 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
17 Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
18 Transfer 20 μl supernatant to a new Hard-Shell PCR plate or to a new 8-tube strip.
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Document # 1000000000753 v00
Amplify Tagmented DNA
Amplify Tagmented DNA
Preparation
1
Save the following LAM AMP program on the thermal cycler:
} Choose the preheat lid option and set to 100°C
} 72°C for 3 minutes
} 98°C for 30 seconds
} 10 cycles of:
} 98°C for 10 seconds
} 60°C for 30 seconds
} 72°C for 30 seconds
} 72°C for 5 minutes
} Hold at 10°C
} Each well or tube contains 50 μl.
Procedure
1
[Plate] Arrange Index 1 (i7) adapters in columns 1–12 of the TruSeq Index Plate
Fixture.
2
[Plate] Arrange Index 2 (i5) adapters in rows A–H of the TruSeq Index Plate Fixture.
3
[Plate] Place the plate on the TruSeq Index Plate Fixture.
4
Add 5 μl of each Index 1 (i7) adapter as follows.
} [Plate] Using a multichannel pipette, add to each column.
} [Tube] Add a different index to each tube.
5
Replace the cap on each i7 adapter tube with a new orange cap.
6
Add 5 μl of each Index 2 (i5) adapter as follows.
} [Plate] Using a multichannel pipette, add to each column.
} [Tube] Add a different index to each tube.
7
Replace the cap on each i5 adapter tube with a new white cap.
8
Add 20 μl LAM, and then mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 1 minute.
} [Tube] Pipette up and down.
9
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
10 Place on the thermal cycler and run the LAM AMP program.
SAFE STOPPING POINT
If you are stopping, seal the plate or cap the tube and store at 2°C to 8°C for up to 2 days.
Alternatively, leave on the thermal cycler overnight.
TruSeq Rapid Exome Library Prep Protocol Guide
5
Clean Up Amplified DNA
Procedure
1
Centrifuge at 280 × g for 1 minute.
2
Transfer 50 μl total volume to a new midi plate or to a new 1.5 ml microcentrifuge
tube.
3
Add 90 μl SPB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
4
Incubate at room temperature for 5 minutes.
5
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
6
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
7
Remove and discard all supernatant.
8
Wash 2 times with 200 μl 80% EtOH.
9
Using a 20 μl pipette, remove residual 80% EtOH.
10 Air-dry on the magnetic stand for 5 minutes.
11 Add 17 μl RSB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
12 Remove from the magnetic stand.
13 Incubate at room temperature for 2 minutes.
14 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
15 Place on a magnetic stand and wait until the liquid is clear (~2 minutes).
16 Transfer 15 μl supernatant to a new Hard-Shell PCR plate or to a new 1.5 ml
microcentrifuge tube or 8-tube strip.
17 Quantify the library using a fluorometric method.
SAFE STOPPING POINT
If you are stopping, seal the plate or cap the tube and store at -25°C to -15°C for up to
14 days.
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Document # 1000000000753 v00
Hybridize Probes
Hybridize Probes
Preparation
1
Save the TRE HYB program on the thermal cycler:
} Choose the preheat lid option and set to 100°C
} 95°C for 10 minutes
} 58°C for 30 minutes
} Each well or tube contains 10 μl.
Pool Libraries
1
Combine 500 ng of each DNA library, making sure that each library has a unique
index.
} If the total volume is > 30 μl, concentrate the pooled sample to 30 μl.
} If the total volume is < 30 μl, increase the volume to 30 μl with RSB.
Procedure
1
Add the following reagents in the order listed to a new midi plate or to a new 1.5 ml
microcentrifuge tube.
} DNA library sample or pool (30 μl)
} BLR (10 μl)
} CEX (10 μl)
2
Mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 1 minute.
} [Tube] Pipette up and down.
3
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
4
Add 125 μl SPB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
5
Incubate at room temperature for 10 minutes.
6
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
7
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
8
Remove and discard all supernatant.
9
Wash 2 times with 200 μl 80% EtOH.
10 Using a 20 μl pipette, remove residual 80% EtOH.
11 Air-dry on the magnetic stand for 10 minutes.
TruSeq Rapid Exome Library Prep Protocol Guide
7
12 Add 7.7 μl EHB1, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
13 Remove from the magnetic stand.
14 Incubate at room temperature for 2 minutes.
15 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
16 Place on a magnetic stand and wait until the liquid is clear (~2 minutes).
17 Transfer 7.5 μl supernatant to a new Hard-Shell PCR plate or to a new thin-wall
PCR tube or 8-tube strip.
18 Add 2.5 μl EHB2, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
19 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
20 Place on the thermal cycler and run the TRE HYB program.
8
Document # 1000000000753 v00
Capture Hybridized Probes
Capture Hybridized Probes
Preparation
1
[Plate] Preheat a microheating system with midi plate insert to 50°C.
2
[Tube] Preheat a heat block to 50°C.
Procedure
1
Centrifuge at 280 × g for 1 minute.
2
Transfer all (~10 μl) to a new midi plate or to a new 1.5 ml microcentrifuge tube.
3
Add 250 μl SMB, and then mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 5 minutes.
} [Tube] Pipette up and down.
4
Incubate at room temperature for 25 minutes.
5
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
6
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
7
Remove and discard all supernatant.
8
Remove from the magnetic stand.
9
Add 200 μl EEW, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 4 minutes. Pipette to resuspend the bead pellet
further.
} [Tube] Pipette up and down.
10 Incubate as follows.
} [Plate] Place on the 50°C microheating system with the lid closed for 30 minutes.
} [Tube] Place on the 50°C heat block for 30 minutes.
11 Immediately place on a magnetic stand and wait until the liquid is clear
(~2 minutes).
12 Remove and discard all supernatant.
13 Remove from the magnetic stand.
14 Repeat steps 9–13 for a total of 2 washes.
15 Mix 28.5 μl EE1 and 1.5 μl HP3, and then vortex.
16 Add 23 μl elution premix, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 2 minutes.
} [Tube] Pipette up and down.
17 Incubate at room temperature for 2 minutes.
18 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
TruSeq Rapid Exome Library Prep Protocol Guide
9
19 Place on a magnetic stand and wait until the liquid is clear (~2 minutes).
20 Transfer 21 μl supernatant to a new midi plate or to a new 1.5 ml microcentrifuge
tube.
21 Add 4 μl ET2, and then mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 1 minute.
} [Tube] Pipette up and down.
22 Add 5 μl RSB, and then mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 1 minute.
} [Tube] Pipette up and down.
23 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
SAFE STOPPING POINT
If you are stopping, seal the plate or cap the tube and store at -25°C to -15°C for up to
7 days.
10
Document # 1000000000753 v00
Perform Second Hybridization
Perform Second Hybridization
Procedure
1
Add the following reagents in the order listed.
} BLR (10 μl)
} CEX (10 μl)
2
Mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 1 minute.
} [Tube] Pipette up and down.
3
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
4
Add 125 μl SPB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
5
Incubate at room temperature for 10 minutes.
6
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
7
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
8
Remove and discard all supernatant.
9
Wash 2 times with 200 μl 80% EtOH.
10 Using a 20 μl pipette, remove residual 80% EtOH.
11 Air-dry on the magnetic stand for 10 minutes.
12 Add 7.7 μl EHB1, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
13 Remove from the magnetic stand.
14 Incubate at room temperature for 2 minutes.
15 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
16 Place on a magnetic stand and wait until the liquid is clear (~2 minutes).
17 Transfer 7.5 μl supernatant to a new Hard-Shell PCR plate or to a new 8-tube strip.
18 Add 2.5 μl EHB2, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
19 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
TruSeq Rapid Exome Library Prep Protocol Guide
11
20 Place on the thermal cycler and run the TRE HYB program.
12
Document # 1000000000753 v00
Preparation
1
[Plate] Preheat a microheating system with midi plate insert to 50°C.
2
[Tube] Preheat a heat block to 50°C.
Procedure
1
Centrifuge at 280 × g for 1 minute.
2
Transfer 10 μl supernatant to a new midi plate or to a new 1.5 ml microcentrifuge
tube.
3
Add 250 μl SMB, and then mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 5 minutes.
} [Tube] Pipette up and down.
4
Incubate at room temperature for 25 minutes.
5
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
6
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
7
Remove and discard all supernatant.
8
Remove from the magnetic stand.
9
Add 200 μl EEW, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 4 minutes. Pipette to resuspend the bead pellet
further.
} [Tube] Pipette up and down.
10 Incubate as follows.
} [Plate] Place on the 50°C microheating system with the lid closed for 30 minutes.
} [Tube] Place on the 50°C heat block for 30 minutes.
11 Immediately place on a magnetic stand and wait until the liquid is clear
(~2 minutes).
12 Remove and discard all supernatant.
13 Remove from the magnetic stand.
14 Repeat steps 9–13 for a total of 2 washes.
15 Mix 28.5 μl EE1 and 1.5 μl HP3, and then vortex.
16 Add 23 μl elution premix, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 2 minutes.
} [Tube] Pipette up and down.
17 Incubate at room temperature for 2 minutes.
TruSeq Rapid Exome Library Prep Protocol Guide
13
Perform Second Capture
Perform Second Capture
18 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
19 Place on a magnetic stand and wait until the liquid is clear (~2 minutes).
20 Transfer 21 μl supernatant to a new midi plate or to a new 1.5 ml microcentrifuge
tube or 8-tube strip.
21 Add 4 μl ET2, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
22 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
14
Document # 1000000000753 v00
Clean Up Captured Library
Clean Up Captured Library
Procedure
1
Add 45 μl SPB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
2
Incubate at room temperature for 5 minutes.
3
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
4
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
5
Remove and discard all supernatant.
6
Wash 2 times with 200 μl 80% EtOH.
7
Use a 20 μl pipette to remove residual EtOH.
8
Air-dry on the magnetic stand until dry (~5 minutes).
9
Add 27.5 μl RSB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
10 Remove from the magnetic stand.
11 Incubate at room temperature for 2 minutes.
12 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
13 Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
14 Transfer 25 μl supernatant to a new Hard-Shell PCR plate or to a new 8-tube strip.
SAFE STOPPING POINT
If you are stopping, seal the plate or cap the tube and store at -25°C to -15°C for up to
7 days.
TruSeq Rapid Exome Library Prep Protocol Guide
15
Amplify Enriched Library
Preparation
1
Save the following AMP10 program on the thermal cycler:
} Choose the preheat lid option and set to 100°C
} 98°C for 30 seconds
} 10 cycles of:
} 98°C for 10 seconds
} 60°C for 30 seconds
} 72°C for 30 seconds
} 72°C for 5 minutes
} Hold at 10°C
} Each well or tube contains 50 μl.
Procedure
1
Add 5 μl PPC.
2
Add 20 μl EAM, and then mix thoroughly as follows.
} [Plate] Shake at 1200 rpm for 1 minute.
} [Tube] Pipette up and down.
3
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
4
Place on the thermal cycler and run the AMP10 program.
SAFE STOPPING POINT
If you are stopping, seal the plate or cap the tube and store at 2°C to 8°C for up to 2 days.
Alternatively, leave on the thermal cycler overnight.
16
Document # 1000000000753 v00
Clean Up Amplified Enriched Library
Clean Up Amplified Enriched Library
Procedure
1
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
2
Transfer 50 μl to a new midi plate or to a new 1.5 ml microcentrifuge tube.
3
Add 50 μl SPB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
4
Incubate at room temperature for 5 minutes.
5
Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
6
Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
7
Remove and discard all supernatant.
8
Wash 2 times with 200 μl 80% EtOH.
9
Use a 20 μl pipette to remove residual EtOH.
10 Air-dry on the magnetic stand until dry (~5 minutes).
11 Add 32 μl RSB, and then mix thoroughly as follows.
} [Plate] Shake at 1800 rpm for 1 minute.
} [Tube] Pipette up and down.
12 Remove from the magnetic stand.
13 Incubate at room temperature for 2 minutes.
14 Centrifuge as follows.
} [Plate] Centrifuge at 280 × g for 1 minute.
} [Tube] Centrifuge briefly.
15 Place on a magnetic stand and wait until the liquid is clear (2–5 minutes).
16 Transfer 30 μl supernatant to a new Hard-Shell PCR plate or to a new 1.5 ml
microcentrifuge tube or 8-tube strip.
SAFE STOPPING POINT
If you are stopping, seal the plate or cap the tube and store at -25°C to -15°C for up to
7 days.
TruSeq Rapid Exome Library Prep Protocol Guide
17
Validate Enriched Libraries
Quantify Libraries
1
Quantify the postenriched library using the Qubit dsDNA BR Assay Kit.
Assess Quality
18
1
If the library concentration is higher than the supported quantitative range for the
High Sensitivity DNA chip, dilute the library 1:10 with RSB.
2
Run 1 μl of post enriched library using a High Sensitivity DNA chip.
Document # 1000000000753 v00
Acronyms
Acronyms
Acronym
Definition
BLR
Blocker
CEX
Coding Exome Oligos
EAM
Enrichment Amplification Mix
EE1
Enrichment Elution Buffer 1
EEW
Enhanced Enrichment Wash Solution
EHB1
Enrichment Hybridization Buffer 1
EHB2
Enrichment Hybridization Buffer 2
ET2
Elute Target Buffer 2
HP3
2N NaOH
LAM
Library Amplification Mix
PPC
PCR Primer Cocktail
RSB
Resuspension Buffer
SMB
Streptavidin Magnetic Beads
SPB
Sample Purification Beads
ST2
Stop Tagment Buffer 2
TD
Tagment DNA Buffer
TDE2
Tagment DNA Enzyme 2
TruSeq Rapid Exome Library Prep Protocol Guide
19
Notes
For technical assistance, contact Illumina Technical Support.
Table 1 Illumina General Contact Information
Website
Email
www.illumina.com
[email protected]
Table 2 Illumina Customer Support Telephone Numbers
Region
Contact Number
Region
North America
1.800.809.4566
Japan
Australia
1.800.775.688
Netherlands
Austria
0800.296575
New Zealand
Belgium
0800.81102
Norway
China
400.635.9898
Singapore
Denmark
80882346
Spain
Finland
0800.918363
Sweden
France
0800.911850
Switzerland
Germany
0800.180.8994
Taiwan
Hong Kong
800960230
United Kingdom
Ireland
1.800.812949
Other countries
Italy
800.874909
Contact Number
0800.111.5011
0800.0223859
0800.451.650
800.16836
1.800.579.2745
900.812168
020790181
0800.563118
00806651752
0800.917.0041
+44.1799.534000
Safety data sheets (SDSs)—Available on the Illumina website at
support.illumina.com/sds.html.
Product documentation—Available for download in PDF from the Illumina website. Go
to support.illumina.com, select a product, then select Documentation & Literature.
TruSeq Rapid Exome Library Prep Protocol Guide
Technical Assistance
Technical Assistance
Illumina
5200 Illumina Way
San Diego, California 92122 U.S.A.
+1.800.809.ILMN (4566)
+1.858.202.4566 (outside North America)
[email protected]
www.illumina.com
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