DNA Engine Thermal Cyclers From the MJ line of amplification products. ®

DNA Engine Thermal Cyclers From the MJ line of amplification products. ®
DNA Engine
Thermal Cyclers
From the MJ line of amplification products.
The MJ Line of Amplification Products
Cyclers That Meet
the Needs of
Every Researcher
DNA Engine
Thermal Cycler
Upgradable to Opticon™
and Chromo4™
real-time systems
Bio-Rad is proud to offer the MJ line of thermal
cyclers, which includes the landmark DNA Engine
family. Since 1988, the MJ brand has become
practically synonymous with thermal cycling.
Having mastered Peltier technology, MJ line cyclers
were the first to deliver excellent thermal control
in a compact and affordable instrument. In fact, the
original Peltier thermal cycler, the PTC-100® cycler,
and later the DNA Engine cycler, established the
design on which most of today’s thermal cyclers
are modeled.
The DNA Engine line is our premium value
instrument platform, incorporating the most
advanced thermal technologies with unparalleled
flexibility. The hallmark of the DNA Engine — the
Alpha unit — allows you to adjust your cycler’s
capacity and vessel accommodation as your
research needs evolve.
Dyad /Dyad Disciple
Cycler Tandem
Choice of two space-saving
DNA Engine family cyclers
DNA Engine Dyad
Thermal Cycler
Advanced graphical interface
Alpha Units
Interchangeable sample blocks
Dyad Disciple
Thermal Cycler
DNA Engine Tetrad 2 Thermal Cycler
Updated version of the cycler that powered
the Human Genome Project
Directed by an attached
Dyad cycler or PC;
upgradable to Chromo4
real-time system
Alpha units
Alpha Units —
the Heart of the
DNA Engine Thermal Cycler
Alpha units are swappable sample-block/heat-pump
assemblies that can be used to customize any DNA
Engine line thermal cycler, and over ten Alpha units have
been developed to accommodate the diverse needs
of modern laboratories. Alpha types vary by number of
sample blocks (one or two), sample capacity, and heated
lid technology — and the new Chromo4 module performs
real-time PCR.
Each Alpha unit contains the thermoelectric heat pumps
and sensors that are required to modulate sample
temperatures, while the thermal cycler base delivers
the electric power and appropriate software control.
Our pioneering multi-sensor, multizone temperature
control provides superb temperature uniformity across
a sample block, producing consistent temperature profiles
across samples and between subsequent runs. Each
Alpha unit is meticulously temperature-qualified using
NIST-traceable standards, ensuring comparable
performance from any Alpha unit, regardless of format
or thermal cycler chassis used. In fact, frequent changing
does not affect thermal performance of Alpha units, and
protocols established on one instrument can be readily
transferred to other DNA Engine line cyclers.
Interchangeable formats for changing demands
Installing an Alpha unit requires no tools — Alpha units can be rapidly exchanged to quickly
accommodate various vessel types and a range of sample capacities.
Standard Alpha Units —
Manual Heated Lids
Slide Chambers™
Alpha Unit
Moto Alpha™
Units — Motorized
Heated Lids
Chromo4™ Detector —
4-Color Real-Time
Standard Alpha units feature adjustable heated lids, which are manually set to optimize the sealing
pressure for diverse types of vessels and sealers. The amount of pressure is adjusted with the use
of a thumbwheel and the lid temperature is set with the thermal cycler software.
Single-Block Alpha Units — 96-well single blocks are gradient capable.
Holds 96 x 0.2 ml tubes, one 96-well plate, or up to 30 x 0.5 ml tubes
Holds one 384-well plate
Holds 60 x 0.5 ml tube
Holds microarrays, biochips, and flat-bottom vessels
Dual-Block Alpha Units — Dual Alpha™ units are independently controllable, so separate protocols may be run side-by-side.
Holds 2 x 48 x 0.2 ml tubes or up to 2 x 12 x 0.5 ml tubes
Holds 30 x 0.5 ml and 48 x 0.2 ml tubes
Holds 2 x 30 x 0.5 ml tubes
Slide Chambers Alpha units thermally cycle glass microscope slides in a dual-block format,
with each block holding up to 16 slides, and are designed for in situ techniques and humidified
chamber hybridizations.
Slide Chambers Dual Block — The two chambers are independently controllable, so separate protocols may be
run side-by-side.
Holds 2 x 16 glass slides
Holds 2 x 16 glass slides, reverse mounted for Tetrad™ cyclers
Moto Alpha units are equipped with motorized heated lids and are designed for automation
integrations, low-volume cycling, and high-throughput applications. The sealing pressure
and opening angle are set with the software and may be controlled remotely from a PC.
Moto Alpha Units — Moto Alpha units are available in single-block formats only; 96-well single blocks are gradient capable.
Holds 96 x 0.2 ml tubes, one 96-well plate, and up to 30 x 0.5 ml tubes
Holds one 384-well plate
Holds microarrays, biochips, and flat-bottom vessels
The Chromo4 module is a real-time detector mounted to a 96-well gradient Alpha unit and
equipped with a user-changeable photonics shuttle for four-color excitation and detection. The
Chromo4 module uses long-lived LEDs for excitation and sensitive photodiodes for detection,
ensuring accurate results from start to finish.
Chromo4 Detector — Requires external PC; gradient capable.
Holds 96 x 0.2 ml tubes or one 96-well plate
Chromo4 real-time PCR detector; includes optical housing and analysis software
CDM-324001 Chromo4 photonics shuttle; contains LEDs, photodiodes, and filters
DNA Engine family cyclers
DNA Engine Line
Thermal Cyclers
DNA Engine
The popular single-bay DNA Engine cycler, the
namesake of the product line, revolutionized
thermal cycling when introduced in 1994. The
DNA Engine was the first to provide a high level
of thermal performance with swappable blocks
and an elegant compact design. The DNA Engine
can be upgraded to a dedicated real-time Opticon
system or be used with the swappable Chromo4
real-time detector.
The dual-bay DNA Engine Dyad thermal cycler features
a high-density graphical interface and color display.
Point-and-click navigation through the software (via
onboard touchpad or external mouse) enables rapid
input of protocols. For laboratories expecting increased
sample throughput, the Dyad can be expanded to a
four-bay system with the addition of the Dyad Disciple
thermal cycler.
DNA Engine line thermal cyclers include 1-, 2-, and 4-bay cycling systems, with
each model delivering equivalent thermal performance. Multi-bay systems allow
each Alpha unit to be independently controlled, and with dual-block Alpha units,
two different protocols can be run side-by-side in a single bay. For instance, a
four-bay Tetrad cycler (when outfitted with four dual-block Alpha units) can run
up to eight independent protocols simultaneously.
Dyad Disciple
The Dyad Disciple thermal cycler converts the Dyad
instrument to a 4-bay thermal cycler, or may be run
independently with a desktop PC. The Dyad Disciple
attaches to a Dyad cycler or a PC running Windows
OS and outfitted with Disciple Desktop™ freeware.
For remote operation in robotic integrations, the Dyad
Disciple is an excellent choice due to its small size and
economical price. The Dyad Disciple can be upgraded
to real-time PCR capability using a Chromo4 detector
with the Opticon Monitor™ software run through an
attached PC.
Tetrad 2
The Tetrad 2 thermal cycler features the advanced
graphical interface found on the Dyad cycler grafted
onto the space-saving Tetrad four-bay design. The
high-capacity Tetrad served as the thermal cycling
backbone for the Human Genome Project. With the
advanced programming interface and increased file
management tools, the Tetrad 2 is the preferred
cycler for high-throughput cycling in both research
and production environments.
Technology for
Unparalleled Results
The integrity of MJ line thermal cyclers rests on the
performance of the Peltier heat pumps (or thermoelectric
modules, TE) that are responsible for delivering rapid and
accurate thermal changes to reaction samples. In order to
maintain the highest TE production guidelines, our engineers
created their own state-of-the-art TE manufacturing facility,
which produces solid-state TE modules that withstand the
thermal stresses associated with rapidly cycling temperatures
and provide years of accurate and reliable thermal performance.
Comparative Lifetime of Peltier TEs
Relative conductance
MJ Line Cyclers Deliver the Best in
Peltier Technology
Extensive life testing, in which TEs are subjected to
repeated temperature cycling until failure, indicates that
MJ brand TEs outperform and outlast any other TE on
the market. The collected data show that TEs in MJ line
cyclers last significantly longer, and decay at a much
slower rate, than some of the best TEs built by
manufacturers throughout the world.
MJ cycler TE’s
Number of cycles
A standard accelerated life test for thermoelectric modules involves
repeatedly cycling a sample block of standard mass. The x-axis represents
the number of cycles and the y-axis represents relative change in
conductance (decreased conductance is a sign of incipient failure). The
various “brands” represent competing premium TEs designed or rated for
thermal cycling.
Block Assembly Diagram of an Alpha Unit
Multizone Thermal Control
Multiple zones of thermal control across sample
blocks enable the DNA Engine line of cyclers to
deliver remarkable well-to-well temperature uniformity
(±0.4°C). Precision Joule heaters adjust the temperature
of perimeter wells — where thermal losses to radiative
heat are the highest — independently of center wells.
Sophisticated algorithms use readings from three
sensors in a single-block Alpha unit to adjust four
independently controlled thermal zones (left, right,
inner, and outer), quickly bringing the sample block
to uniformity even when samples are asymmetrically
loaded. Multizone control consistently delivers
thermal uniformity.
Sample block
Joule heaters
Heat sink
Precision Joule heaters adjust perimeter wells where thermal losses
to radiative heat are the highest. Multiple sensors and independently
controlled TEs compensate for asymmetrically loaded samples.
As an alternative, DNA Engine line cyclers also offer a
calculated temperature control mode. With this option,
the software asks the user to input the sample volume
and vessel type to account for the correct amount of
temperature lag. The microprocessor then calculates
an optimized thermal profile and directs the block
to overshoot its target slightly; it starts timing when
the sample temperature itself reaches the set point
(lower panel, opposite). By removing some guesswork,
calculated control can reduce both optimization time
and overall run time. Calculated control is recommended
for most applications.
Block Temperature Control
Temperature, ˚C
Time, sec
Calculated Temperature Control
Temperature, ˚C
“Block” vs. “Calculated” Temperature Control
The DNA Engine line of thermal cyclers offers two modes
of controlling sample temperatures. With the simplest
method, block temperature control mode, the instrument
monitors the temperature of the block and precisely heats
and cools as directed. However, when the temperature
within a sample is measured, an almost asymptotic lag
can be noticed as heat is transferred from block to vessel
to sample (upper panel, opposite).
Time, sec
Temperatures were ramped from 60–90˚C using a 96-well Alpha unit
on a DNA Engine cycler with 0.2 ml tubes and a 25 μl sample volume.
Accurate incubation times are crucial for successful transfer
of protocols from a gradient to a nongradient mode.
Temperature, ˚C
Dynamic Ramping in Gradient Mode
Dynamic Ramping and the Gradient Feature
Multizone technology allows DNA Engine line cyclers to
create a highly reproducible temperature gradient across
the sample block using software alone. The gradient
feature allows optimization of reactions in a single
experiment using a range of temperatures simultaneously.
Temperature gradients ranging from 1˚C up to 24˚C can
be programmed across a 96-well sample block. The
gradient feature employs dynamic ramping, which means
that the temperature gradient forms during ramping. All
wells come to their designated temperature at the same
time, making the incubation period consistent among all
samples in the experiment.
Time, sec
Four thermal cyclers were programmed to develop a 45–65°C gradient
across 12 columns. Thermal measurements were taken with NIST-traceable,
laser-trimmed thermistors from 48 wells (4 wells/column) in each cycler.
Mean temperature of each column in each cycler (48 total traces) is plotted.
Note that the software adjusts ramp rates so that all samples reach the
incubation temperature at the same time.
Automation solutions
Low-Volume Cycling
and Robotics
The MJ line is a top choice for high-throughput and automated environments.
DNA Engine line cyclers boast the smallest footprint available, are readily
networked, and can be remotely operated using a universal set of ASCII
commands. Over the years, our scientists have partnered with many leading
robotics manufacturers to offer the most flexibility for the incorporation of
thermal cyclers into spatially constrained workstations.
Moto Alpha unit and Remote
Alpha Dock system (inset)
For especially tight integrations, we’ve developed the Remote Alpha Dock™
(RAD) system which allows an Alpha unit to be operated up to 3 m away
from any DNA Engine line cycler chassis. The two-bay Dyad Disciple cycler
is particularly suited for automation — having the smallest possible per-bay
dimensions. Historically, the Tetrad has been the favored instrument for
high-throughput laboratories (with a 1,536-sample capacity when equipped
with four 384-well Alpha units), while the DNA Engine cycler remains very
popular for lower-throughput robotic applications.
The Moto Alpha Unit
The Moto Alpha unit is the last element needed for
fully automated thermal cycling — offering remote lid
opening and closing, high-pressure sealing, and a
number of other robot-friendly features. When used
with Hard-Shell® microplates and Microseal® 'P+'
sealing pads, optimal performance can be expected.
Moto Alpha features include:
Motorized heated lid can be operated directly
or remotely
High sealing pressure is applied evenly across
every well
Active pressure sensing ensures repeatability
Low volume (1–5 μl) cycling can be performed reliably
Stainless lifter springs present the microplate 5 mm
above the block
Sealing pressure and opening angle are set
with software
Key Accessories for Automation
Hard-Shell 96- and 384-well microplates contain a
rigid skirt designed to resist warping and shrinkage
due to the thermal stresses of high-temperature
cycling. The absence of plate
deformation ensures reliable
robotic gripping of plates,
while maintaining the flatness
and well spacing necessary
for reproducible pipetting.
Bio-Rad’s arched auto-sealing lids allow simple
automation of sealing, opening, and resealing plates
without the need to integrate costly automated sealing
and unsealing workstations. Arched auto-sealing lids
automatically release from microplates when the cycler
is opened, and therefore are ideal for repeated access.
In contrast, flat auto-sealing lids remain tightly sealed
to the microplate for up to 24 hours after cycling, and
can be used for short-term storage down to –20˚C.
The lids contain sealing pads that are easily cleaned
between uses, and are reusable up to 50 times. For
cycling of low-volume reactions (1–3 μl), auto-sealing
lids form an extremely tight seal and reliably seal when
used with the Moto Alpha motorized lid. Evaporative
losses of low-volume samples during dispensing are
also reduced 5-fold by placing an auto-sealing lid over
a reaction plate as soon as the plate is filled.
Microseal 'P+' pads use the same sealing pad as the
auto-sealing lids, but in a format that can be adhered
directly to the cycler lid. Ejector pins in the lid ensure
that the plate will not stick to the lid when the cycler
is opened.
Real Time
Real-Time PCR in
1, 2, or 4 Colors
Real-time quantitative PCR is a highly sensitive technology that combines
DNA amplification with simultaneous process monitoring. Accumulation of
labeled product is optically monitored as the amplification reaction progresses.
By comparing the rates of product accumulation between a set of known
standards and a set of experimental samples, the initial quantities of template
in the unknown samples can be assessed. Real-time analysis is a powerful
technique used in many applications including, gene expression profiling,
quantification of DNA and RNA targets, microbial detection, and viral
load determination.
real-time system
Opticon Systems
The Opticon and Opticon 2 systems are dedicated
real-time detectors that are built around the DNA Engine
cycler with a 96-well gradient Alpha unit. These two
systems offer one- and two-color detection respectively.
Opticon instruments feature a unique optical detection
system incorporating fixed-LED excitation and
photomultiplier tube detection that provides a broad
linear dynamic range of detection, including detection
of single-copy targets, without any moving parts.
The Chromo4 Detector
The four-color Chromo4 fluorescence detector allows
configuration of a DNA Engine line thermal cycler
into a real-time system. The Chromo4 detector, which
is mounted to a swappable 96-well gradient Alpha
unit, permits multicolor detection for multiplexing or
for detection of a variety of targets using various dye
chemistries simultaneously. The compact Chromo4
module features a photonics shuttle that can be ordered
with customized filter sets, tailoring the detection
capability specifically to your needs.
Reaction Vessels and Sealers
Bio-Rad offers a full line of top-quality reaction vessels and sealing systems for thermal cycling
applications. DNA Engine line cyclers accommodate a variety of reaction vessels depending
on the chosen Alpha unit. Whether you require 0.5 ml or 0.2 ml tubes, high-density microplates,
or glass microscope slides, Bio-Rad can outfit you with the appropriate solution for each. A full
selection of reliable sealing systems is available for each vessel and can be custom selected
based on vessel type, cycling application, and storage method.
0.5 ml
Full-Height Low-Profile Multiplate™ Low-Profile Microseal
Microseal Hard-Shell Concord™
0.2 ml
0.2 ml
Unskirted Multiplate
Tubes and Strip Tubes Microplates Unskirted Semi-Skirted
Strip Tubes
Microplates Microplates Microplates Microplates Microplates
Microseal Hard-Shell
Microplates Microplates
Alpha block sizes
60, 30
96, 48, 32
96, 48, 32
96, 48, 32
96, 48, 32
Thermal cycling
reaction volumes
10–200 µl
5–125 µl
5–125 µl
5–125 µl
5–125 µl
5–125 µl
5–125 µl
5–125 µl
20–150 µl
5–25 µl
3–30 µl
Maximum well volume
600 µl
250 µl
200 µl
330 µl
200 µl
330 µl
220 µl
250 µl
210 µl
40 µl
50 µl
Suggested when using
fewer than 96 wells
Recommended for
use with Opticon and
Chromo4 systems
Thermal cycling
reaction volume
'A' Film
'B' Seals
Sealing Mat
'F' Foil
'P' Pads
≥10 µl*
≥5 µl**
≥10 µl*
≥5 µl**
≥10 µl
≥25 µl*
≥5 µl**
≥15 µl*
≥10 µl**
Recommended for
use with Opticon and
Chromo4 systems
Domed Optical, Flat Frame-Seal™ Hyb-Seal™
'P+' Pads†/ Liquid Wax Strip Caps Strip Caps Chambers
Auto-Sealing Overlay
≥5 µl*
≥1 µl**
Compatible with
cold storage
Seal can be pierced
Suggested for
sealing tubes
Seals 384-well
† Designed for use with the new motorized Moto Alpha unit.
* Applies to 96-well format
** Applies to 384-well format
≥5 µl
25, 60, 125,
or 300 µl
165, 355,
or 465 µl
15–150 µl
Seals glass slides
≥5 µl
sealing possible
Reusable sealer
≥2 µl
Alpha Unit Specifications
Gradient Specifications (96-Well Alpha Units Only)
Thermal range
0–105˚C (4–100˚C for
Slide Chambers unit)
Speed of ramping
Up to 3˚C/sec (up to 1.2˚C/sec for
Slide Chambers unit)
Temperature accuracy
±0.3˚C of programmed target at 90˚C
(±0.4˚C for dual-block Alpha units and
Slide Chambers unit); NIST-traceable
Temperature uniformity
Lid options
Gradient accuracy
±0.3˚C of programmed target at end
columns within 30 sec after gradient
step timer starts; NIST-traceable
Column uniformity
±0.4˚C, well-to-well within column, within
30 sec of reaching target temperature
Gradient calculator accuracy ±0.4˚C of actual well temperature
±0.4˚C well-to-well within 30 sec of arrival
at 90˚C (±0.5˚C for dual block Alpha units;
±0.6˚C well-to-well within 60 sec of arrival
at 90˚C for Slide Chambers unit)
Lowest temp for gradient
Highest temp for gradient
Temperature differential range 1–24˚C
Motorized or manual, all heated
and adjustable
Instrument Specifications
DNA Engine
Thermal Cycler
Number of cycling bays
DNA Engine Dyad
Thermal Cycler
Dyad Disciple
Thermal Cycler
DNA Engine Tetrad 2
Thermal Cycler
Input power
100–240 VAC, 50–60 Hz,
850 W max
200–240 VAC, 50–60 Hz,
1,600 W max, fitted with
NEMA L6-20P plug
200–240 VAC, 50–60 Hz,
1,600 W max, fitted with
NEMA L6-20P plug
200–240 VAC, 50–60 Hz,
3,200 W max, fitted with
NEMA L6-20P plug
9 kg (chassis and Alpha unit)
16.8 kg (chassis and two
96-well Alpha units)
10.1 kg (chassis only)
21.6 kg (chassis only)
Size (W x D x H)
24 x 35 x 25 cm,
chassis and Alpha unit
48 x 29 x 21 cm,
chassis and Alpha units
46 x 28 x 21 cm,
chassis and Alpha units*
47 x 61 x 21 cm,
chassis and Alpha units
20 x 4 alphanumeric LCD
320 x 240 pixels, 256 colors
No display
320 x 240 pixels, 256 colors
Mouse controllable
IEEE-488 bidirectional
general-purpose interface
bus, 9-pin RS-232 serial
port for printer or remote
use, 25-pin 8-bit parallel
interface printer port
RS-232, Ethernet,
Dyad Disciple expansion
2 USB ports (1 per bay),
Dyad expansion
RS-232, Ethernet
Program capacity
400 typical programs;
up to 12 passwordprotected folders
1,000 typical programs
No intrinsic memory —
relies on Dyad or
external PC
1,000 typical programs
All DNA Engine line thermal cyclers offer: Peltier-effect thermal cycling technology, choice of temperature control mode, interchangeable Alpha units with
heated lids, gradient capability (when outfitted with 96-well Alpha units), power failure restore, networking capability and an Instant Incubation option.
* Overall size of a four-bay Dyad/Dyad Disciple system — stacked configuration with rack accessory, 48 x 44 x 62 cm (W x D x H) with Alpha units; linear
configuration with baffle accessory, 105 x 29 x 21 cm (W x D x H) with Alpha units.
The MJ Line of Amplification Products
Ordering information
Catalog #
Catalog #
DNA Engine Thermal Cycler Chassis
DNA Engine Thermal Cycler Chassis
(does not include the Alpha unit — requires 1)
Cycling Accessories
Remote Alpha Dock System, for DNA Engine line
(requires fan power supply)
DNA Engine Dyad Thermal Cycler Chassis
(does not include the Alpha units — requires 2)
Dyad Disciple Thermal Cycler Chassis
(does not include the Alpha units — requires 2)
DNA Engine Tetrad 2 Thermal Cycler Chassis
(does not include the Alpha units — requires at
least 2 and fits up to 4)
Baffle Accessory, for a Dyad Disciple system
(linear configuration)
Dyad Disciple USB Accessory Kit, connects 2
Disciple cyclers to a single PC. Includes USB hub
and 5 USB cables
Dyad Disciple Power Strip Accessory Kit, allows 2
Dyad Disciple systems to operate from a single 220 V
outlet; includes powerstrip and 2 power cords
Available Alpha Units
Single Alpha Unit, holds 60 x 0.5 ml tubes
Single Alpha Unit, holds 96 x 0.2 ml tubes or
one 96-well plate
Single Alpha Unit, holds one 384-well plate
Single Alpha Unit, holds microarrays, biochips,
flat-bottom vessels
Dual Alpha Unit, holds 2 x 30 x 0.5 ml tubes
Dual Alpha Unit, holds 2 x 48 x 0.2 ml tubes
or half-plates
Dual Alpha Unit, holds 30 x 0.5 ml and
48 x 0.2 ml tubes
Dual Alpha Unit, holds 2 x 16 glass slides
(for DNA Engine, Dyad, Dyad Disciple cyclers)
Dual Alpha Unit, holds 2 x 16 glass slides
(for Tetrad cyclers)
Moto Alpha Unit, holds 96-well plate, with
integrated motorized heated lid
Moto Alpha Unit, holds 384-well plate, with
integrated motorized heated lid
Flat Block Moto Alpha Unit, customizable flat
surface block with four screw-down points, with
integrated motorized heated lid and flat inner lid
Flat Block Moto Alpha Unit, customizable flat
surface block with four screw-down points, with
integrated motorized heated lid and skirted inner lid
Chromo4 Real-Time PCR System, includes
optical housing and analysis software (requires
additional products)
Fan Power Supply, for Remote Alpha Dock system
Dyad Disciple Accessories
Rack Accessory, for a Dyad Disciple system
(stacked configuration)
For more information on other products featured in this
brochure, including the Chromo4 detector and Opticon
real-time systems and MJ reaction vessels and sealers,
visit our web site at www.bio-rad.com
Practice of the patented polymerase chain reaction (PCR) process requires a license.
The DNA Engine line of thermal cyclers, the PTC-100 thermal cycler, and the Opticon
and Opticon 2 systems include an Authorized Thermal Cycler, and may be used with
PCR licenses available from Applied Biosystems. Their use with Authorized Reagents
also provides a limited PCR license in accordance with the label rights accompanying
such reagents. Some applications may also require licenses from other third parties.
Appearances and specifications are subject to change without notice.
Windows is a trademark of Microsoft Corporation.
Laboratories, Inc.
MJ Products web site www.mjr.com Corporate web site www.bio-rad.com USA (888) 735-8437 Australia 02 9914 2800 Austria (01)-877 89 01
Belgium 09-385 55 11 Brazil 55 21 2527 3454 Canada (905) 712-2771 China (86 21) 6426 0808 Czech Republic + 420 2 41 43 05 32
Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65 Germany 089 318 84-0 Greece 30 210 777 4396 Hong Kong (852) 2789 3300
Hungary 36 1 455 8800 India (91-124)-2398112/3/4, 5018111, 6450092/93 Israel 03 951 4127 Italy 39 02 216091 Japan 03-5811-6270
Korea 82-2-3473-4460 Latin America 305-894-5950 Mexico 55-52-00-05-20 The Netherlands 0318-540666 New Zealand 64 9 415 2280
Norway 23 38 41 30 Poland + 48 22 331 99 99 Portugal 351-21-472-7700 Russia 7 095 721 1404 Singapore 65-64153188 South Africa 00 27 11 4428508
Spain 34 91 590 52 00 Sweden 08 555 12700 Switzerland 061 717 95 55 Taiwan (886 2) 2578 7189/2578 7241 United Kingdom 020 8328 2000
Life Science
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