O&M Manual for NuAire NU-602 Animal Handling Biosafety Cabinet

O&M Manual for NuAire NU-602 Animal Handling Biosafety Cabinet
Labgard Class II, Type A2 Laminar Flow
Biological Safety Cabinet
Models
NU-602-4XX/5XX/6XX
NU-602-4XXE/5XXE/6XXE
Console
Operation & Maintenance Manual
October, 2011
(Series 25)
Revision 3
115 VAC Only
115 VAC Only
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
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Congratulations!
You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available.
With proper care, maintenance (certification), and laboratory procedure, this cabinet will give you years of
product and personnel protection from particulate contaminants as prescribed in National Sanitation Foundation
(NSF) Standard No. 49. Please read this manual carefully to familiarize you with proper installation,
maintenance and operation of the cabinet. Other reference and guideline materials are available through the
following web sites.
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absa-canada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.osha.gov/dts/osta/
www.nuaire.com
Acknowledgment
NuAire, Inc. acknowledges that some material in this manual reflects information supplied by the National Institutes of Health personnel both in
verbal and written specifications. In particular, NuAire acknowledges that information in Section 8 was obtained from the following sources:
1. Technical Report No. FPS 56500000001. Prepared by Dow Chemical Co., for the National Cancer Institute, May 1, 1972.
2. Stolar MH, Power LA, Vielo CS: Recommendations for handling cytotoxic drugs in hospitals. Am J Hosp Pharm 1983;40:
1163-1171.
3. Anderson R.W., Director of Pharmacy, University of Texas, M.D. Anderson Hospital and Tumar Institute at Houston.
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ABOUT THIS OPERATION & MAINTENANCE MANUAL
The information contained in this manual is intended to reflect our current production standard
configuration model along with the more frequently purchased options. Any unique additions, modifications,
shop drawings are appended in the back flap of this manual, along with any modifications and/or additions to
procedures as outlined in this manual. A copy of the original factory test report is also appended to this manual.
In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement
copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief
description of the information desired.
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Labgard Class II, Type A2
Laminar Flow Biological Safety Cabinet
Models NU-602-4XX/5XX/6XX
NU-602-4XXE/5XXE/6XXE
Operation & Maintenance Manual
TABLE OF CONTENTS
Section No. 1 ...................................................................... General Description
Section No. 2 ...................................................................... Models and Features
2.1 ................................................................... Optional Systems
Section No. 3 ...................................................................... Warranty
Section No. 4 ...................................................................... Shipments
Section No. 5 ...................................................................... Installation Instructions
5.1 ....................................................................... Location
5.2 ....................................................................... Set-up Instructions
5.3 ....................................................................... Certification Testing Methods and Equipment
Section No. 6 ...................................................................... Operating the NU-602
6.1 ....................................................................... Operator Controls & Indicators
6.2 ....................................................................... Auto-Lift Operating Controls
6.3 ....................................................................... Operating Guidelines
6.4 ....................................................................... Ergonomics
6.5 ....................................................................... Operating Sequence
6.6 ....................................................................... Cleaning Procedure
Section No. 7 ...................................................................... General Maintenance
7.1 ....................................................................... Decontamination
7.2 ....................................................................... Fluorescent Lamp Bulb Replacement
7.3 ....................................................................... HEPA Filter/Motor Replacement
7.4 ....................................................................... Auto-Lift System
7.5 ....................................................................... Airflow Calibration
7.6 ....................................................................... Filter Integrity Check
7.7 ....................................................................... Cabinet Leak Tightness Test
Section No. 8 ...................................................................... Error Indicators & Troubleshooting
Section No. 9 ...................................................................... Remote Contacts
Section No. 10 .................................................................... Optional Equipment
10.1 ..................................................................... Ultraviolet Light
Section No. 11 .................................................................... Electrical/Environmental Requirements
Section No. 12 .................................................................... Disposal and Recycle
Insert ....................................................................................... Replacement Parts List
MANUAL DRAWINGS
BCD-05658 ................................................... NU-602 Airflow Schematic
BCD-05436 ................................................... NU-602-400/400E
BCD-05437 ................................................... NU-602-500/500E
BCD-05438 ................................................... NU-602-600/600E
BCD-05440 ................................................... NU-602 w/Sterile Cage Delivery Option
BCD-05441 ................................................... NU-602 w/Dirty Cage Collection Option
BCD-05439 ................................................... NU-602 w/Refuse Disposal Option
BCD-01816 ................................................... Service Control Center Installation
BCD-06719 ................................................... Control Panel
ASSEMBLY DRAWINGS
ACD-04275................................................... Exhaust Filter Guard Installation
BCD-00919 ................................................... Filter Replacement Procedure
BCD-05457 ................................................... Auto-Lift Castor Base Assembly
ELECTRICAL DRAWINGS
BCD-13237 ................................................... NU-602-400/500/600 Electrical Schematic
BCD-13238 ................................................... NU-602-400E/500E/600E Electrical Schematic
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Labgard Class II, Type A2
Laminar Flow Biological Safety Cabinet
Animal Cage Changing Station
Models
NU-602-4XX/5XX/6XX
NU-602-4XXE/5XXE/6XXE
Manufactured By:
NuAire, Inc. - Plymouth, Minnesota
1.0
General Description
The LABGARD Model NU-602 Laminar Flow Biological Safety Cabinet (LFBSC) is highly similar to
the NuAire Model NU-407 but slightly modified to better serve the personnel in animal research. The NU-602
has passed the performance criteria of NSF 49 in tests conducted at NuAire, Inc. in Plymouth, MN.
The Laminar Flow Biological Safety Cabinet, (LFBSC) when used with proper technique, is an effective
laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of
both animal and personnel to airborne biological or particulate chemical agents in low to moderate risk-hazard
research and drug preparation or product operations, as prescribed by the Center for Disease Control (CDC)
Atlanta, Georgia.
In order to meet the special needs of the science of animal research, the NU-602 includes the following
significant features:
1.
2.
3.
4.
Greater visibility because the fluorescent lamp lighting the interior of the cabinet is placed
inside the back of the work zone. A shield prevents direct viewing of the lamp. Thus, the full
window is clear for viewing the work area.
Larger work opening access under the viewing window to the work zone is increased to 12
inches (305mm).
Autolift System with heavy duty stainless steel castors have been integrated into the base, yet
keeping the overall cabinet height at 77.50 (1968mm) inches. At this height, the cabinet can be
rolled through most doorways. The cabinet is very easy to move and rise to variable working
heights for user ease.
Prefilter is placed ahead of the HEPA filters to remove hair and dander, prolonging HEPA life.
The prefilter is easy to service.
In addition, to the foregoing, three significant systems are offered as purchasable options which greatly
improve work flow, increase flexibility and improve cleanliness in the handling of the animals:
• Sterile Cage Delivery Option
• Dirty Cage Collection Option
• Refuse Disposal Option
The NU-602 console LFBSC is known as a Class II, type A2 Biological Safety Cabinet. This is possible
since NuAire's cabinet conforms to the following requirements:
1.
2.
3.
4.
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Maintains a minimum average inflow velocity of 100 LFPM through the work access opening.
Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust
plenum.
Exhaust airflow can either be room re-circulated or exhausted outside using a canopy exhaust
transition.
Has all biologically contaminated ducts and plenums under negative pressure or surrounded by
negative pressure (type A1 permits positive pressure contaminated ducts and plenums).
5
1.2
Safety Instructions
These safety instructions describe the safety features of the LABGARD Model NU-602 LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been
thoroughly tested before delivery. It may, however, present potential hazards if it is not used according
to the intended purpose or outside of operating parameters. Therefore, the following procedures must
always be observed:
• The safety cabinet must be operated only by trained and authorized personnel.
• For any operation of this unit, the operator must prepare clear and concise written instructions
for operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and
technical regulations, in particular.
o which decontamination measures are to be applied for the cabinet and accessories
o which protective measures apply while specific agents are used
o which measures are to be taken in the case of an accident
• Repairs to the device must be carried out only by trained and authorized expert personnel.
• Keep these operating instructions close to the unit so that safety instructions and important
information are always accessible.
• Should you encounter problems that are not detailed adequately in the operating instructions,
please contact your NuAire Representative of NuAire technical Services.
1.3
Explanation of Symbols
!
WARNING
WARNING indicates a potentially hazardous
situation which, if not avoided, could result in
death of serious injury.
!
CAUTION:
CAUTION indicates a potentially hazardous
situation which, if not avoided, may result in
minor or moderate injury.
CAUTION
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation which, if
not avoided, may result in property damage.
Potential electrical hazard, only qualified person
to access.

NOTE:
Flammable Hazard
Used for important information.
Biohazard
Hazardous Gases!
Personal Protection
Equipment Required.
Ground, Earth
Chemical Hazard
Lead Free
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2.0
Models & Features
The Model NU-602, Class II, Type A2 Laminar Flow Biological Safety Cabinet is manufactured in three
sizes: 4 ft. (0.9m), 5 ft. (1.2m), and 6 ft. (1.8m).
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2.1
Optional Systems
The NU-602 Biological Safety Cabinet can be purchased with several operational systems which can
greatly improve work flow, increase flexibility, and improve cleanliness in the handling of small
animals. These systems are limited to factory installation and must be purchased when the basic cabinet
is purchased. These systems are identified as the following:
1.
2.
3.
Sterile Cage Delivery Option
Dirty Cage Collection Option
Refuse Disposal Option
2.1.1
Sterile Cage Delivery Option
The NuAire Model NU-602 is offered with a Sterile Cage Delivery option. Space requirements
for this system limit installation to the 6 foot cabinet. Sterile cages are delivered under wraps to
the cage changing station and placed in the delivery system where wraps are removed, twelve or
more mice cages or 6 rat cages will maintain sterility because they are constantly "bathed" in
HEPA filtered air (99.99% efficient for removal of 0.3 micron particles). The delivery system
contains a spring balanced platform that moves vertically to place a sterile cage(s) at a position
where they can easily be picked up by an operator. As each cage is removed from the stack of
cages on the platform, the platform rises to place another sterile cage in position to be easily
picked up. The HEPA filtered air flows down (around) the cages continuously drawn finally
into the cabinet blower system where 70% of the air is recycled through the supply HEPA filter
and 30% is exhausted to the room through an exhaust HEPA filter (see Drawing BCD-05440).
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2.1.2
Dirty Cage Collection Option
The NuAire Model NU-602 is offered with a Dirty Cage Collection Option (see Drawing BCD05441). This option permits an operator to change animals from a dirty cage to a clean cage and
place the dirty cage (complete with old bedding, etc.) through a door in a wall of the cabinet into
a bag for autoclaving. Thus, the operator has the personnel protection of a biological safety
cabinet, the animal is protected from contamination and the soiled cages are dispensed in a
convenient manner ready for sterilizing (animal cages are not furnished with this option). This
option can be manufactured into either both left or right sides of the cabinet and is available in
all three sizes.
Typically, a stack of sterile cages would be placed on the right side of the work tray. In the
biological cabinet, a dirty cage complete with the mouse would be brought into the biological
cabinet work zone on the left side (one-at-a-time). The mouse would be transferred from the
dirty cage to a clean cage which would have been serviced as necessary. The dirty cage would
then be placed into the collection bag to be autoclaved.
The operator can build a stack of 8 dirty cages placing them one-at-a-time into the autoclave
bag.
The cage support platform is adjustable up/down, but the nominal setting of the platform is 8-1/2
inches (216mm) below the bag attachment. Subsequent cage placing procedures may dictate a
more comfortable position to the operator.
The autoclave bag is stripped over the bag attachment; tight fit is desirable, but even a slightly
large bag can be held in place with a bit of tape in a few places while the "O" ring performs the
final retaining action.
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2.1.3
Refuse Disposal Option
The NuAire Model NU-602 is offered with a Refuse Disposal Option. Space requirements for this
system limit the installation to the 6 foot (1.8m) cabinet. The disposal function is displayed on the left
side work tray with the right side of the work tray used for processing the animal cages. Refuse is
dumped into a double bag system, with the outer bag fastened to the underside of the plenum below the
work tray and outer bag fastened to a metal ring member that fits snugly into the work tray. The ring
member is lifted out of the work tray to permit an operator to fasten the inner bag. Both bags fit snugly
to metal rings, but are also retained by "O" rings. The diameter of both bag rings is approximately
14-1/2" (368mm) or 45" (1143mm) in circumference. The outer bag seals the interior of the cabinet from
the room and the inner bag carries the refuse. In preparing to dispose of the refuse, the inner bag is
released from the metal ring in the work tray and dropped into the outer bag. Then the outer bag is
released, tied and the refuse can is transported to an autoclave for sterilization of the bags. NuAire does
not supply replacement bags. Replacement bags may be obtained, using the above ring specifications, in
various laboratory supply catalogs.
Typically, dirty cages with animals would be brought into the station on the left side. Clean cages would
be stacked in the station on the right side having been autoclaved with bedding already installed.
Animals would then be transported one at a time into the clean cages and the dirty cage would be
dumped into the refuse bag. The dirty cage would then be subjected to normal procedure cleaning and
sterilization (see Drawing BCD-05439).
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3.0
Warranty
NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a
similar part to replace any material in its equipment within 36 months after the date of sale if proved to
the satisfaction of the company to have been defective at the time it was sold provided that all parts
claimed defective shall be returned, properly identified to the company at its factory, charges prepaid.
Factory installed equipment or accessories are warranted only to the extent guaranteed by the original
manufacturer, and this warranty shall not apply to any portion of the equipment modified by the user.
Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of the
defect, the date of the initial installation and the serial and model number of the equipment.
This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse,
abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts of
God. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty
shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior
to returning any item, for any reason, contact NuAire for a Return Authorization Number. This number
must accompany all returns. Any product shipped to NuAire without this number will be returned
refused shipment or collect freight.
4.0
Shipments
NuAire takes every reasonable precaution to assure that your LABGARD cabinet arrives without
damage. Motor carriers are carefully selected and shipping cartons have been specially designed to
insure your purchase. However, damage can occur in any shipment and the following outlines the steps
you should take on receipt of a NuAire LABGARD cabinet to be sure that if damage has occurred, the
proper claims and actions are taken immediately.
4.1
Damaged Shipments
4.1.1
Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment
before acceptance.
4.1.2
If there is visible damage, the material can be accepted after the driver makes a notation on the
consignee's copy of the freight bill. Then an inspection must be made to verify the claim against
the carrier. This inspection is the basis of your filing the claim against the carrier.
4.1.3
If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT
AT ONCE and, request an inspection. Without this inspection, the transportation company may
not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit
must be prepared stating that he was contacted on a certain date and that he failed to comply
with the request. This along with other papers in the customer's possession will support the
claim.
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5.0
Installation Instructions
5.1
Location
Within the laboratory, etc., the ideal location of the biological safety cabinet is away from personnel
traffic lanes, air vents (in or out), doors and/or any other source of disruptive air currents.
If drafts or other disruptive air currents exceed the intake velocity of the cabinet through the access
opening, the potential exists for contaminated air to exit or enter the work tray area of the cabinet. It
depends on the severity of the air current. REMEMBER: A BIOLOGICAL SAFETY CABINET IS
NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.
W Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for
maintenance purposes. The electrical outlet into which the cabinet is connected should be readily
accessible for maintenance purposes. Do not position the cabinet to prevent access to the power
cord. The power cord plug serves as the disconnect and should remain readily accessible. If the outlet
is inaccessible, such as a conduit (hardwired) connection, then an appropriate warning label should be
applied near the cabinets on/off switch to indicate the circuit breaker on the power distribution panel to
be used. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE
CABINET TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX.
If it is desired to exhaust the cabinet to the outside, NuAire offers two general categories of exhaust
transitions which will capture the exhaust efflux from the cabinet. These are:
1. Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan) Strongly Recommended
2. Gas-Tight Exhaust Transitions
THE EXHAUST SYSTEM SHOULD BE FITTED WITH A BACKDRAFT
 NOTE:
DAMPER TO PREVENT REVERSING OF AIRFLOW IN THE SYSTEM.
Both types of transitions have some common attributes, in addition to some that are unique. NuAire
strongly recommends a canopy or thimble exhaust for most applications. See separate instruction
sheets for a discussion of exhaust transitions and installation requirements.
5.2
Set-Up Instructions
Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is
usually the best procedure to leave the skid in place until the cabinet is located in its approximate
position to facilitate ease in handling. It can then be removed from the skid by removing the brackets,
bolts and screws holding the cabinet to the skid.
The unit is shipped for on-site assembly of the following item:
Exhaust Filter Guard
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!
5.2.1
Exhaust Grill Installation
The exhaust HEPA filter is protected with a cardboard cover for shipping. Remove the cover
and install the expanded metal exhaust guard to the exhaust chamber with 1/4-20 acorn nuts and
washers provided. If an exhaust transition is being installed, the guard is not required.
5.2.2
Gas Service
NuAire doesn't recommend the use of natural gas within the LFBSC, but if gas service is
determined to be necessary for the application, appropriate safety measures must take place.
All NuAire LFBSC's have precautionary warning labels that say the following:
CAUTION
Use of explosive or flammable substances in this cabinet should be evaluated by your
appropriate safety personnel.
Once the determination has been made by the appropriate safety personnel, the application of
natural gas must be performed in accordance to national, state and local codes. IT IS ALSO
STRONGLY RECOMMENDED THAT AN EMERGENCY GAS SHUTOFF VALVE BE
PLACED JUST OUTSIDE THE LFBSC ON THE GAS SUPPLY LINE.
All NuAire LFBSC's meet the safety requirements of UL and CSA for Laboratory Equipment.
To comply with these safety requirements, NuAire uses only certified gas valves. In addition, if
external piping is required, only black pipe is used for this application.
As previously stated NuAire doesn't recommend the use of natural gas within the LFBSC and
ASSUMES NO RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The
Bunsen burner flame within the LFBSC not only contributes to heat build-up; is also disrupts the
laminar air stream, which must be maintained for maximum efficiency. IF THE
PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON
DEMAND IGNITION IS STRONGLY RECOMMENDED. DO NOT USE CONSTANT
FLAME GAS BURNERS. During use, the Bunsen burner should be placed to the rear of the
workspace where resulting air turbulence will have a minimal effect.
5.2.3
Plumbing Services
Ground key cocks with the type of service specified by the removable button on the handle, are
located in the work zone. The Ground Key cocks are not recommended for pressure over 30 psi
(2.0 BAR). Reducing valves should be installed external to the cabinet if necessary. Ground
key cocks should never be used for flammable gasses or oxygen service. A special needle for
oxygen service or certified valve is required and available upon request.
External connection is to 3/8” (38mm) NPT coupling in the inner sidewalls. Plugged clearance
holes in the outer sidewalls are provided for easy access to the inner couplings via a NPT nipple.
Connection to plant utilities should be made with proper materials for the individual service and
according to national and/or local codes. Observe all labels pertaining to the type of service and
operating pressure.
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5.2.4

Electrical Services
The NU-602 series Biological Safety Cabinets may be "hardwired" (optional) or connected via
an electrical power cord which is standard. The unit requires 115 or 230 VAC, 60 or 50Hz,
single phase (current rating varies per cabinet size, reference Electrical/Environmental
Requirements). It is recommended that power to the unit be on its own branch circuit, protected
with a circuit breaker or fuse at the distribution panel.
NOTE: THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE FLUORESCENT
LIGHTING. ELECTRONIC BALLASTS OPERATE WITH HIGH INRUSH CURRENT.
IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH GROUND FAULT
INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO
TRIP.
5.2.5
 NOTE:
Final Assembly
REMOVE THE PROTECTION CARDBOARD COVER OVER THE EXHAUST HEPA,
located under the protective screen. The exterior surfaces and viewing glass are easily cleaned
with any mild household detergent cleaner using a soft cloth. Harsh chemicals, solvent-type
cleaners and abrasive cleaners should not be used.
Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily
cleaned with any mild household detergent cleaner using a soft cloth. Turn the cabinet on and
let it operate for 60 minutes before using it as a LFBSC.
5.3
Certification Testing Methods and Equipment
After installation and prior to use, NuAire recommends that the cabinet be certified or commissioned to
factory standards. At a minimum, the following tests should be performed.
1.
2.
3.
4.
HEPA filter leak test
Downflow velocity test
Inflow velocity test
Airflow smoke patterns
The testing methods and equipment required are specified on the factory inspection report included with
this manual (see insert in back cover).
 NOTE:
IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED
TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR
CERTIFYING BIOLOGICAL SAFETY CABINETS (SEE INSERT).
THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE
 NOTE: AFTER
CABINET BE RECERTIFIED AT A MINIMUM ON AN ANNUAL BASIS AND AFTER
EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME THE
OPERATOR FEELS IT IS NECESSARY.
Note that the Labgard cabinets, filters and seals provide premium performance; Quality Control in both
design and manufacturing assure superior reliability. However, protection to both product and operator
is so vital that certification to the performance requirements should be accomplished as stated to ensure
biological safety established by the factory standards.
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Labgard Class II, Type A2
Laminar Flow Biological Safety Cabinet
Models NU-602-4XX, 5XX, 6XX
NU-602-4XXE/5XXE/6XXE
Catalog Number
Performance Specifications
1. Personal Protection
2. Product Protection
NSF Std. No. 49 Class
Style of Cabinet
Cabinet Construction
Pressure Tight Design
Diffuser for Air Supply (Metal)
HEPA Seal Type:
Supply Filter-99.99% Eff. on 0.3 microns
Exhaust Filter-99.99% Eff. on 0.3 microns
Fumigation per NIH/NSF Procedure
Standard Services:
Gas Cocks 3/8" NPT
Duplex Outlet
Optional Services:
Gas Cocks 3/8" NPT
Duplex Outlets
Ultraviolet Light
Cabinet Size: inches (mm)
Height
Width
Depth
Work Access Opening:
Standard Opening Height - inches (mm)
Standard Inflow Velocity (DIM)
Work Zone: inches (mm)
Height
Width
Depth
Viewing Window: inches (mm)
Hinged Tempered Glass
Minimum Exhaust Flow Standard
Plant Duct Static Pressure
Heat Rejected, BTU, Per Minute
Electrical: 115V (230V)
Volts, AC 60/50 Hz
Amps: Blower/Lights
Amps: Duplex
Amps: Total
12 ft. Power Cord (one)
*Crated Shipping Weight:
Net Weight
Catalog Number
NU-602-5XX
Nominal 5 foot (1.5m)
NU-602-4XX
Nominal 4 foot (0.9m)
NSF/ANSI 49
NSF/ANSI 49
Class II, Type A2
Console
All welded stainless steel
16GA, Type 304
14GA CRS Base
Non-flammable
NSF/ANSI 49
NSF/ANSI 49
Class II, Type A2
Console
All welded stainless steel
16GA, Type 304
14GA CRS Base
Non-flammable
NSF/ANSI 49
NSF/ANSI 49
Class II, Type A2
Console
All welded stainless steel
16GA, Type 304
14GA CRS Base
Non-flammable
HEPEX Seal*
Neoprene, Springloaded
Yes
HEPEX Seal*
Neoprene, Springloaded
Yes
HEPEX Seal*
Neoprene, Springloaded
Yes
One, Right Sidewall
One, Right Sidewall
One, Right Sidewall
One, Right Sidewall
One, Right Sidewall
One, Right Sidewall
Up to 3 ea. Sidewall
1 ea. Sidewall
One, Backwall
Up to 3 ea. Sidewall
1 ea. Sidewall
One, Backwall
Up to 3 ea. Sidewall
1 ea. Sidewall
One, Backwall
77 (1956)
50 1/2 (1283)
31 3/4 (806)
77 (1956)
62 1/2 (1588)
31 3/4 (806)
77 (1956)
74 1/2 (1588)
31 3/4 (806)
12 (305)
105 FPM (.53m/s)
12 (305)
105 FPM (.53m/s)
12 (305)
105 FPM (.53m/s)
26 1/2 (673)
46 1/2 (1232)
24 1/2 (622)
26 1/2 (673)
58 1/2 (1486)
24 1/2 (622)
26 1/2 (673)
70 1/2 (1791)
24 1/2 (622)
12 (305mm) opening
407 CFM (629CMH)
0.05-0.1" H20
12 (305mm) opening
512 CFM (870 CMH)
0.05-0.1" H20
12 (305mm) opening
617 CFM (1048CMH)
0.05-0.1" H20
24
UL/UL-C Listed (115V only)
115/60 (230/50,60)
10 (7)
3 (3)
13 (10)
12 GA - 3 Wire, 20A
815 lbs.
780 lbs.
35
UL/UL-C Listed (115V only)
115/60 (230/50,60)
12 (8)
3 (3)
15 (11)
12 GA - 3 Wire, 20A
915 lbs.
880 lbs.
43
UL/UL-C Listed (115V only)
115 (230/50,60)
12 (8)
3 (3)
15 (11)
12 GA - 3 Wire, 20A
1015 lbs.
980 lbs.
*Crated shipping weight does not include weight for accessories or options.
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NU-602-6XX
Nominal 6 foot (1.8m)
23
6.0
Operating the NU-602
6.1
Operator Controls & Indicators
The following is a description of the controls and indicators, left-to-right on drawing BCD-06719.
6.1.1
Minihelic Gauge
The unit is normally equipped with a minihelic gauge. The minihelic gauge displays the static
pressure within the pressure plenum supplying the downflow and exhaust filter. The gauge is
calibrated in "inches of water gauge" or "millimeter of water gauge" pressure. As the HEPA
filters load with particulate matter, the amount of static pressure will increase, giving an
indication of the "health" of the cabinet. The initial pressure reading will be approximately
0.50" w.g. ± 0.05" w.g. (12mm w.g. ± 3mm w.g.).
6.1.2
Indicator Light
An indicator light is located above the blower on/off switch and lights when power is applied to
the motor blower.
6.1.3
Blower Switch
The blower switch applies power to the internal blower/motor when in the "ON" position. The
blower switch also has a second set of poles, which are available for use as a contact closure for
an exhaust system. In addition, power is applied to the accessory outlet located in the rear of the
service control center.
The purpose of the outlet is to provide "switched" power for the following:
1.
2.
Exhaust transition with integral blower, or
To power a low power appliance, such as an Airflow Monitor.
The rating of the outlet is 115 Vac at 2 amps maximum. The 230 Vac cabinets do not include
this outlet as standard.
6.1.4
Fluorescent/U.V. Light Switch
This switch provides on/off control for the fluorescent light and/or the ultraviolet (U.V.) light if
present (optional). With the U.V. light option, the switch provides for on-center off-on operation
so that both the fluorescent light and the U.V. light cannot be energized at the same time. Proper
care should be exercised when the U.V. light is on.
6.1.5
Circuit Breaker-Blower
The Blower is protected with a circuit breaker. The circuit breaker in conjunction with the
motor's thermal protector is designed to open under locked rotor or half-wave power conditions.
Should the circuit breaker open (pop-out button will appear) merely depress to reset. If the
circuit breaker continually opens, a failure has occurred in the motor or solid-state speed
controller. Consult a qualified repair technician or NuAire, Inc. for replacement.
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24
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25
6.1.6
Outlet Switch
This switch provides on/off control for the 115 or 230 Vac power available in the outlet within the
interior or the cabinet.
6.1.7
Circuit Breaker-Outlets
The outlet located in the sidewall of the work area is protected with a 3 amp circuit breaker. The
circuit breaker may trip at 110% of load rating but will trip at 145% of load rating in less than 2
seconds. Should the circuit breaker open, (pop-out button will appear), unplug the appliance
plugged into the outlet and merely depress the pop-out button to reset.
6.1.8
Airflow Control
The operating airflows within the cabinet (i.e. 75 LFPM (.38 m/s) downflow and 105 LFPM
(.53 m/s) air inflow barrier) are controlled by a potentiometer and an exhaust damper. The
potentiometer is located under the hinged cover of the service control center, in the left-rear
corner. The shaft of the potentiometer has been painted red for easy identification.
The potentiometer controls the operating voltage applied to the motor/blower. The potentiometer
is adjustable over 270 degrees with a slotted screwdriver, which varies the applied voltage. This
adjustment should only be made by a qualified technician employing a thermoanemometer or
velometer in order to insure airflows.
6.2
Auto-Lift Operating Controls
The following is a description of the controls and indicators (see Drawing BCD-05456).
6.2.1
Power Switch
The power switch applies power to the auto-lift control system. A green light within the switch
indicates an "ON" state.
6.2.2
Mode Switch
The mode switch has three modes of activation, full down, run and lock. The full down mode is
used to return the Auto-Lift down to its lowest position from any raised position. The run mode
is used to alter the Auto-Lift up or down into a desired position. The lock mode is used to lock
the Auto-lift system in any raised position.
6.2.3
Worksurface Height Switch
The worksurface height switch allows for the precise selection of the worksurface height in
inches (cm). The worksurface height switch is used in conjunction with the mode switch in the
run position. Changes to the worksurface height can be made at anytime.
CAUTION:
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Lift system is designed for a 10% duty cycle with a maximum on time of
2 minutes.
26
6.3
Operating Guidelines
The intent herein is to present general operational guidelines that will aid in the use of the Laminar Flow
Biological Safety Cabinet (LFBSC) to control airborne contaminants of low to moderate risk as stated in
Technical Report No. FPS 56500000001, prepared by Dow Chemical U.S.A., for the National Cancer
Institute, May 1, 1972.
Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be
developed in order to obtain a maximum potential for safety and protection. The pre-planning necessary
to develop these protocols is based on several fundamental considerations, each of which will contribute
to optimum benefits from the equipment:
a.
b.
c.
d.
e.
Know your "Safe Working Area"
Minimize disruption of "air curtain"
Minimize room activity
Utilize unidirectional airflow
Employ aseptic techniques
6.3.1
Know Your "Safe Working Area"
The LFBSC safe working area is basically the worktray or depressed area. All work
should be performed on or above the worktray. The area on or above the front grill is a
non-safe working area.
6.3.2
Minimize Penetration of "Air Curtain"
The minimum number of items necessary should be placed into the cabinet to prevent
overloading, but the work should also be planned to minimize the number of times an operator's
hands and arms must enter and leave the air curtain at the open face. The ideal situation is to
have everything needed for the complete procedure placed in the hood before starting, so that
nothing need pass in or out through the air barrier at the face until the procedure is completed.
This is especially important in working with moderate risk agents.
Unnecessary rising of the hands inside the cabinet above the level of the work opening should be
avoided. This presents an inclined plane from hands to elbows along which the downflow of air
may run to, and possibly out, the open face.
 NOTE:
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When working with agents of lower risk, it is not as important for all materials to be placed in
the cabinet before starting, or for the procedure to be completely finished before materials are
removed. Also, the time period for a unit may be continued over a more extended period during
which entries and withdrawals from the cabinet may be made.
27
6.3.3
Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may create
disruptive air currents as well as interfere with the work of the operator. A person walking past
the front of a cabinet can cause draft velocities up to 175 fpm, which are sufficient to disrupt the
air balance of the laminar flow unit.
6.3.4
Utilize Unidirectional Air Flow
The operator must keep two important facts in mind: (1) The air, as supplied to the work area
through filters from the top, is contaminant free and (2) Airborne contamination generated in the
work area is controlled by the unidirectional flow of parallel air streams in a top-to-bottom
direction.
A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the
capability of controlling lateral movement of airborne particulates. A cone of turbulence extends
below the object and laminarity of the air stream is not regained until a point is reached
downstream, approximately equal to three to six times the diameter of the object. Within the
parameters of this cone, particles may be carried laterally by multidirectional eddy currents.
Transfer of viable materials and manipulations which may generate aerosols should not be
performed above sterile or uninoculated materials. Items should be localized on the work
surface in "clean" and "dirty" groups.
6.3.5
Employ Aseptic Technique
The operator must not assume an attitude of "let the cabinet do it" when performing procedures
within a LFBSC. Properly balanced and properly used cabinets will do an excellent job of
controlling airborne contamination and containing viable agents, but the cabinet will not
eliminate contact transmission of contamination. Normal laboratory contamination control
procedures and basic aseptic techniques are necessary to obtain maximum benefit from the
cabinet. For example, open bottle, tube or flask mounts should be kept as parallel as possible to
the downflow to minimize capture of chance particulates. This precaution is merely an
extension of good aseptic technique as practiced on open bench tops. The good laboratory
practices designed to minimize creation and/or release of aerosols to the environment should not
be discontinued.
Items of equipment in direct contact with the etiologic agent must remain in the cabinet until
enclosed or until surface-decontaminated. Trays of discard pipettes must be covered before
removal from the cabinet (aluminum foil may substitute for fabricated covers).
If an accident occurs which spills or splatters suspensions of etiologic agent around the work
area, all surfaces and items in the cabinet must be surface-decontaminated before being
removed.
Applying a burner flame to flask and tube necks when mating surfaces of sterile assemblies is a
conventional method of minimizing chance contamination. However, the efficiency of this
operation is usually related to the removal of airborne contamination occurring while the item is
uncovered. If the manipulation is carried out in an environment free of airborne particulates,
then the need for the flaming operation is essentially removed. This is one of the additional
advantages of the LFBSC - use of the gas burner is seldom necessary. The gas burner flame in
one of these units not only contributes significantly to the heat build-up, it also disrupts the
laminar air streams which must be maintained for maximum efficiency.
!
CAUTION IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON
DEMAND IGNITION IS RECOMMENDED. DO NOT USE CONSTANT FLAME GAS
BURNERS. It should also be placed to the rear of the work space where resulting air turbulence
will have a minimal effect. If cabinet air is inadvertently turned off, the flame could damage the
HEPA filters.
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28
6.4
Ergonomics
Ergonomics, the study or accommodation of work practices is extremely important for proper cabinet usage
and user health and safety. An evaluation of normal work practices should be performed with each user when
working in a cabinet. Evaluation criteria should be at a minimum:
a. Proper user posture
b. Effective workzone layout for work practice
c. Vision or sightlines
For each of the above evaluation criterion, several aids may be supplied to accommodate the user.
• Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to assure
proper user posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure
back is fully supported with proper chair adjustments.
• Forearm/elbow support - The cabinet is provided with a forearm support on the work access opening.
Periodic mini-breaks during work practice should be taken resting forearm to avoid stress and fatigue.
Elbow rests are optional that can provide support for particular work practices, such as pipetting.
• Effective workzone layout - Always prepare your work procedure to minimize reach to avoid neck and
shoulder stress and fatigue. Rotating tables are optional to maximum workzone and minimize reach.
Vision and sightline - Always prepare your work procedure to eliminate glare and bright reflections on the
window. Keep your window clean and sightlines clear to your effect workzone.
6.5
Operating Sequence
6.5.1
Start Up
Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet to make
sure they are unobstructed. Many of the cabinets are provided with magnehelic gauges which
indicate pressure differentials across the supply filters. They may indicate to the LFBSC
maintenance technician when to replace the filters, dependent upon the blower fan capacity.
Blower speed must only be readjusted by qualified maintenance technicians.
Note: Some cabinets are equipped with ultraviolet (UV) lights. These must be turned off
during the day while laboratory personnel are occupying the room. Good procedure
includes the decontamination or wipedown of cabinet surfaces with chemical
disinfectant before work commences. This practice eliminates the need for UV lights,
whose primary utility in this application is inactivation of surface contamination since
the filters effectively remove all airborne contaminants. UV lights, therefore, are not
recommended in the LFBSC.
Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun
in the cabinet. If the filtered air exhausted from the unit is discharged into the room, as in some
installations, an additional advantage is obtained from purification (filtration) of the room air
circulated through the equipment. Because of this characteristic contributing to the quality of
the laboratory environment, some owners of LFBSC leave them in operation beyond the time of
actual use.
6.5.2
Wipedown
The interior surfaces of the work space should next be disinfected (see cleaning procedures) by
wiping them thoroughly with 70% alcohol or similar non-corrosive antimicrobial agents. DO
NOT USE ANY CHLORINATED OR HALOGEN MATERIALS IN THE CABINET.
6.5.3
Materials & Equipment
The apparatus and materials should next be placed into the cabinet. Care must be exercised that
no items be placed over the front intake grills. Materials should be arranged so that clean, dirty
(used), and virus materials are well separated. Passage of contaminated materials over
uninoculated cultures or clean glassware should be avoided and transfer of viable materials
should be performed as deeply into the cabinet (away from open face) as possible.
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6.5.4
Air Purge
Additional purging of the work space without user activity should be allowed for 2-3 minutes
after materials and apparatus have been placed in it. This will rid the area of all "loose"
contamination that may have been introduced with the items.
6.5.5
Perform Work
The work can now be performed. The technician performing the work is encouraged to wear a
long-sleeved gown with knit cuffs and rubber gloves. This will minimize the shedding of skin
flora into the work area and concurrently protect the hands and arms from viable agent
contamination. At a minimum, the hands and arms should be washed well with germicidal soap
before and after work in the cabinet. For the preparation of antineoplastic drugs, the following
procedures summarize those contained in OSHA Instruction PUB 8-1.1, "Work Practice
Guidelines for Personnel Dealing with Cytotoxic (Antineoplastic) Drugs." The above document
should be thoroughly studied / reviewed prior to drug preparation in the cabinet.
a.
b.
c.
d.
e.
A sterile plastic-backed absorbent drape should be placed on the work surface during
mixing procedures. The drape should be exchanged whenever significant spillage
occurs, or at the end of each production sequence.
Vials should be vented with a filter needle to eliminate internal pressure or vacuum.
Before opening ampules, care should be taken to insure that no liquid remains in the tip
of the ampules. A sterile gauze sponge should be wrapped around the neck of the
ampules while opening.
Final drug measurement should be performed prior to removing the needle from the
stopper of the vial.
A non-splash collection vessel should be available in the biological safety cabinet to
discard excess drug solutions.
6.5.6
Terminal Purging & Wipedown
Following completion of work, allow the cabinet to run for 2-3 minute period without personnel
activity to purge the unit. The decontamination of the interior surfaces should be repeated after
removal of all materials, cultures, apparatus, etc. A careful check of grills and diffuser grids
should be made for spilled or splashed nutrients which may support fungus growth and resulting
spore liberation that contaminates the protected work environment.
6.5.7
Prefilter
Located under the work surface is a prefilter area. This area forms the return air path to the
motor/blower. If the air flow is blocked by paper or the prefilter is "loaded", the performance of
the cabinet could be seriously affected. Therefore, THE PREFILTER SHOULD BE
CHECKED AND CLEANED NO LESS THAN ON A WEEKLY BASIS; on a daily basis if
procedures dictate the use of paper products. Any paper removed must be properly disposed of
as Contaminated Hazardous Waste.
6.5.8
Shut Down
Turn off blowers and lights. Do not use cabinet as a depository for excess lab equipment during
periods of non-operation. If the antineoplastic agents are being prepared in the cabinet, it is
recommended to let the cabinet run 24 hours per day. This lessens the possibility that
contaminants may escape.
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6.6
Cleaning Procedures
Cleaning the cabinet is an important function in terms of both containment and sterility. Use the
following procedure to effectively surface disinfect or clean the cabinet workzone surfaces.
a.
b.
CAUTION:
c.
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Raise the window to a full-open position, if desired.
Apply appropriate disinfecting solution to cabinet surfaces. Most surface disinfectants require a
specific contact time depending upon the microbiological agents used within the cabinet.
CONSULT APPROPRIATE DISINFECTANT DOCUMENTATION FOR PROPER
APPLICATION AND SAFETY PRECAUTIONS.
DISINFECTANTS THAT USE CHLORIDES AND HALOGENS WILL CAUSE
DAMAGE TO THE STAINLESS STEEL SURFACES IF LEFT ON FOR LONG
PERIODS OF TIME.
After the specified contact time, wipe up excess disinfectant. IF THE DISINFECTANT
USED CONTAINS CHLORIDES OR HALOGENS, RE-WIPE ALL SURFACES WITH
70% ALCOHOL OR SIMILAR NON-CORROSIVE ANTI-MICROBIAL AGENT TO
PREVENT DAMAGE TO STAINLESS STEEL SURFACES.
31
7.0
General Maintenance
!
ATTENTION ACCOMPANY'S
INFORMATION OR IMPORTANT
SYMBOL
POTENTIAL ELECTRICAL
HAZARD ONLY QUALIFIED
PERSON TO ACCESS
188
191
CAUTION:
7.1
All maintenance actions on this equipment must be performed by a qualified technician
who is familiar with the proper maintenance procedures required for this equipment. This
includes both certification as well as repair.
Decontamination
No maintenance should be performed on the interior of the Labgard cabinet (area behind access panels)
unless the cabinet has been microbiologically decontaminated, is known to be biologically clean, or
known to be chemically inert. Surface disinfection is performed as specified in the cleaning procedures.
!
CAUTION! Hazardous Gases! Personal Protection Equipment Required.
A disinfection using formaldehyde must be performed in accordance with the specifications of
NSF 49/1992, Annex G.
This procedure presents considerable risks and must be performed only by specially trained
and authorized service personnel in accordance with applicable safety regulations.
The formaldehyde is vaporized within the tightly sealed sample chamber. The quantity of the
applied formaldehyde depends on the volume of the sample chamber in the safety cabinet that is
to be disinfected. The formaldehyde evaporates immediately after reaching its boiling point; the
minimum reaction time is 6 hours. Therefore, the formaldehyde should be neutralized after the
specified reaction time by vaporizing ammonia.
!
CAUTION! Flammable Hazard!
Formalin is flammable. The auto-ignition temperature of formalin is 430° C (820° F).
With a volume percentage of 7.75% in dry air, formaldehyde vapor may explode.
For vaporization, do not use heating devices reaching temperatures above 250° C (477° F).
!
CAUTION! Chemical Hazard!
Formalin in reaction with hydrogen chloride will form BCME which is a hazardous chemical.
When using formalin, all residues of hydrogen chloride in the work chamber of the cabinet must
be removed.
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32
If microbiological decontamination is necessary, use the following procedure:
1.
2.
Remove hinged window if desired.
Place decontamination equipment inside the work area. Reference decontamination procedure,
per NSF Standard 49, Annex G, using the following chart to calculate chemical requirements.
Cabinet Size
Approximate Cabinet
Dimensions
Cabinet
Volume
-4XX/E
-5XX/E
-6XX/E
70-1/2 H x 30D x 46-1/2W
(1.79 x .762 x 1.18m)
56.9 cu. ft.
(1.61 cu. m)
70½H x 30D x 58-1/2W
(1.79 x .762 x 1.49m)
71.6 cu. ft.
(2.03 cu. m)
70½ x 30D x 70-1/2W
(1.79 x .762 x 1.79m)
86.3 cu. ft.
(2.44 cu. m)
Note the outlets in the work area are energized as long as the cabinet is plugged in and switched on the
front panel. Unplug the cabinet before decontamination equipment is plugged into these outlets or run
the decontamination power cords under the front seal area.
3.
Use duct tape and plastic to seal the front and exhaust area.
CAUTION:
4.
BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY
LABORATORY EXPOSURE TO DECONTAMINATION GAS.
Perform decontamination procedure per NSF Standard 49, Annex G.
If the cabinet has been used to prepare antineoplastic drugs, (chemotherapy), or other toxic chemicals,
decontamination of the cabinet cannot be accomplished by the above procedure. It is recommended that
the following protective measures be taken:
1.
2.
3.
4.
5.
Gloves
Gloves must be worn. Care must be taken not to cut, puncture, or tear the gloves. No one glove
material is impervious to all CYTAs; disposable surgical or polyvinyl chloride (PVC) gloves
provide substantial but not complete protection. PVC gloves probably are more protective than
surgical gloves, but they are stiffer and less tactile. Gloves should be discarded after each use.
Gloves should be tucked into the cuffs of the gown. Double gloving should be considered.
Face & Eye Protection
A disposable dust and mist respirator and either a plastic face shield (preferred) or chemical
splash goggles must be worn. The face shield or goggles should be wiped clean with a suitable
tissue and water after each use.
Gowns
A protective garment must be worn. The garment should be made of lint-free, low-permeability
fabric and must have a closed front, leg sleeves, and elastic or knit closed cuffs. Tyvek1
isolation gowns are one example of an acceptable garment. The garment must be worn outside
the work area. Disposable gowns are preferred over reusable. Front-buttoned coats are not
recommended.
Hair & Shoe Covers
Disposable hair and shoe covers should be worn.
Motion
Slow and deliberate motions are necessary when working in the interior of the cabinet, in order
to minimize the generation of particulates.
Please consult with NuAire, Inc. about any unique contamination problems.
Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area behind the
access panel containing the HEPA filters and motor (blower assembly). All required adjustments in
order to maintain proper cabinet airflows are external to the cabinet interior. The motor is lubricated for
life and is thermally protected with automatic reset.
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33
7.2
Fluorescent Lamp Bulb Replacement
The bulb is fluorescent, cool white, high output (HO), rapid start and placed internal to the cabinet. The
life rating of the bulb is 9000 hours based on three-hour burning cycles.
To replace a bulb, it is necessary to remove the bulb shield.
1.
2.
3.
7.3
First, switch Cabinet Light Switch off.
The bulb is removed by displacing the bulb to one side against the compressible bulb holder.
Reverse the procedure to install the new bulb.
HEPA Filter/Motor Replacement (Drawing BCD-00919)
The HEPA Filters under normal usage and barring an accident (a puncture), do not need replacement
until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100
LFPM (.51 m/s) (min.). This may permit the average downflow velocity to be as low as 70 LFPM (.35
m/s) as long as no point falls below 20 percent of the average downflow velocity.
The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to
be biologically "clean".
7.3.1
Procedure (see Drawing BCD-00919)
CAUTION:
Disconnect electrical power from the unit before attempting any maintenance action.
Step 1: Remove the exhaust filter access cover and the supply filter access cover, by removing
all acorn nuts, washers and lockwashers (many places).
The interior of the cabinet is now fully exposed for replacement of the filters.
Step 2: Filter Removal
It is not always necessary to replace both the supply and exhaust filter at the same time
although it may be cost effective to do so. If during the course of certifications, the
downflow always falls off while the exhaust increases, the supply filter is "loading"
faster than the exhaust filter and only the supply filter may need replacement. The
opposite might also happen depending upon many factors.
a.
To remove the supply HEPA:
1.
2.
b.
First, release the pressure clamps (3 for 4 foot (.9m) and 5 for 5 and 6 foot (1.2m
and 1.8m)). The clamps provide very high tension and may require mechanical
assistance to unfasten.
Second, remove the filter. The plenum frame is hinged in the rear and must be
raised slightly while removing the HEPA filter. Handles are provided in the
front of the filter.
To remove the exhaust HEPA:
Release the tension on the exhaust filter clamps by turning the knobs clockwise (4
places). This will lower the pressure plenum exhaust frame to a level that permits
removal of the HEPA.
!
CAUTION:
Dispose of spent HEPA filters properly. Avoid direct contact to "dirty side" of the
filters. Label toxic waste.
1 Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributors.
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34
Step 3: Filter Installation
WHEN INSTALLING NEW FILTERS, USE ONLY FILTERS OF THE SAME
RATED, FLOW AND SIZE AS ORIGINALLY INSTALLED. Both filters (supply
and exhaust) require top and bottom gaskets which should be 1/4 inch (6mm) thick,
closed all neoprene. A light coating of silicone or Vaseline grease should be applied to
all gaskets. Care must be taken to assure that the filters are not punctured or the gaskets
damaged when reinstalling the filters. Remove the handles from the front of the dirty
filters and install on the new filters.
a.
Supply filter installation:
Insert the supply filter through the front opening, tilting the filter upward to allow rear
edge of filter to slide on the bottom rail, until the front edge of the filter rests on the front
rail. Install the front clamps with flanged tip insert into the slot in front rail.
b.
Exhaust filter installation:
Before installing the exhaust filter be sure to open the choke plate fully. This will assist
in adjusting the airflow. Next, slide the exhaust filter all the way to the rear of the
pressure frame. Turn the knobs evenly, counterclockwise until an even seal is obtained.
Compress the gaskets about 1/8 inch (3mm), top and bottom.
Step 4: Motor/Blower Assembly Removal
a. The motor/blower is accessible by removing the front access plate in the cabinet base.
7.4
1.
Remove front access plate via #8-32 screws on cabinet base.
2.
Remove the HEPEX bag from the blower via the band clamp.
3.
Disconnecting the electrical connections from the junction box on the
blower housing or bulkhead connector on sidewall of base cabinet.
4.
Remove the blower from the cabinet base via the four #1/4-20 nuts.
5.
Re-install the new motor/blower assembly.
Auto-Lift System (Drawing BCD-05457)
The Auto-Lift has been factory set to adjust the worksurface height between 31 inches (787mm) and
38 inches (965mm). However, due to variances of the floor surface, both at NuAire's factory and at the
installation site, some adjustments may be required. Please observe some items before any work is
attempted on the Auto-Lift System.
1.
Always begin making adjustments from the 31 inch (787mm) worksurface height.
2.
Always use caution and observe safety measures when performing maintenance on the
cabinet.
3.
Never allow the unit to rise higher than 39 inches (991mm) measured at the work surface height.
4.
Never leave hands, feet, or toes under the unit, especially when lowering cabinet.
7.4.1
Unbinding Telescoping Members
a.
Due to floor difference between factory and installation site, the guides for the
telescoping members may require re-alignment.
b.
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Please note that although these parts aren't holding the cabinet up, they do prevent
wobbling, so use caution not to move the cabinet when adjusting.
35
7.4.2
Actuator Replacement
a.
Remove side and bottom cover panels via fasteners on cabinet front, rear &
bottom.
b.
If possible, lower cabinet to its lowest position and block cabinet to take load off
the castors.
c.
Remove power from the cabinet.
CAUTION:
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THE BOTTOM PLATE WITH THE CASTOR ATTACHED WEIGHS
NEARLY 50 POUNDS. USE EXTRA CARE NOT TO DROP IT ON
HANDS OR FEET.
d.
Remove cotter pins and clevis pins at top and bottom, disconnect wires at
interconnect and remove motor.
e.
Clamp base plate to cabinet base.
f.
Install new actuator into position by attaching top pin first, then, rotate bottom tube into
position for bottom pin attachment.
g.
Re-attach bottom and side cover panels.
36
7.5
Airflow Calibration
The NU-602 Airflow Calibration Consists of adjustments to balance the airflow within the cabinet.
THIS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN
 NOTE: MEASURE
THE AIRFLOW FROM THE FILTERS WITH A SUITABLE VELOMETER.
NuAire provides two adjustments to balance the airflow within the cabinet. These are:
a.
b.
blower speed adjustment via motor voltage regulator
exhaust filter choke
The blower speed controller adjusts the cabinet’s total volume of airflow while the choke adjusts or
balances the exhaust airflow as well as makes up for filter resistance tolerances. Since it has been
NuAire's experience that the filters may not "load" evenly, both adjustments are necessary for proper
cabinet performance.
The cabinet is considered to be certifiable if the following airflow measurements are present:
a.
Downflow Average:
75 LFPM ± 5 LFPM (.38 m/s ± .025 m/s).
b.
Inflow Average:
105 LFPM ± LFPM (.53 m/s ± .025 m/s) using the calculated
inflow velocity measurement method.
STARTING AIRFLOW CALIBRATION PROCEDURE LET THE CABINET RUN
 NOTE: BEFORE
FOR AT LEAST 10 MINUTES.
7.5.1 Downflow Calibration
Step 1:
• Place a velometer in the cabinet workzone on the horizontal plane defined by the
bottom edge of the viewing window. Spot check several points on the
recommended downflow velocity test grid found in table 7.0.
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Step 2:
• If necessary, adjust airflow control potentiometer, located in the control center to
the above stated airflow requirements.
Step 3:
• Proceed to inflow calibration
37
7.5.2
Inflow Calibration
Step 1:
• Measure the inflow velocity using the recommended procedure found in Table
7.0. If necessary, adjust the exhaust filter choke, located under the front
decorative panel, to achieve the correct average inflow velocity within the stated
range of 105 ± 5 LFPM (.53 ± .025 m/s).
• Less than 100 LFPM (.51 m/s);
First, open the choke plate or make sure it is open. If this is insufficient, then
increase the motor speed control.
• Greater than 110 LFPM (.56 m/s);
First, adjust the motor speed control to achieve 1/2 the exhaust excess, then close
the choke plate to achieve the balance. In this fashion, the downflow should
remain nearly constant (i.e. what the reduced speed took away, the choke plate
restores).
Note:
Step 2:
7.6
The choke plate adjustment requires a 3/16 inch (5mm) Allen wrench with a 6
inch (152mm) length. While monitoring the exhaust flow to check position,
turning the set screw clockwise will open the choke while turning the set screw
counter clockwise closes the choke.
• Once exhaust adjustment is complete, return the downflow calibration and then
check average downflow velocity. If the downflow average remains within the
correct range, the calibration is complete. If not, readjust as necessary to obtain
the correct calibration range.
Filter Integrity Check
In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly
accessible, by the measuring instrument.
The diffuser plate placed below the HEPA to protect the filter during normal usage may be removed as
follows: The diffuser is secured to the cabinet shell by fasteners located immediately behind the front
viewing window. After removing the fasteners, drop the front of the diffuser plate several inches and
pull forward gently. Note that the diffuser is purposely a tight fit - it is secured to the back wall of the
cabinet interior by a light push - fit with projecting studs.
The exhaust filter is typically more difficult to check, since charcoal filters, or exhaust transitions could
cover the filter. Access panels are usually provided and should be removed. If an air gap exhaust
transition is provided, the air gap must be sealed with duct tape or other suitable means to prevent
contaminated air from migrating into the exhaust efflux. All exhaust blowers/fans should be turned off
during the check.
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Table 7.0
Recommended Measurement Methods for Cabinet Downflow & Inflow.
A.
Downflow Measurement
a.
Instruments: TSI 8355 Thermoanemometer
b.
Procedure: Supply filter efflux is measured on a grid, in a horizontal plane defined by the
bottom edge of the window. No readings should be taken closer than 6 inches (152mm) from
the inside perimeter.
c.
Test Data - Inches (mm):
4XX/E
5XX/E
6XX/E
6
(152)
6
(152)
6
(152)
11.750
(298)
11.812
(300)
11.850
(301)
17.500
(445)
17.625
(448)
17.700
(450)
23.250
(591)
23.437
(595)
23.550
(598)
29.000
(737)
29.250
(743)
29.400
(747)
34.750
(883)
35.062
(891)
35.250
(895)
40.500
(1029)
40.875
(1038)
41.100
(1044)
46.687
(1186)
46.950
(1193)
52.500
(1334)
52.800
(1341)
58.650
(1490)
64.500
(1638)
6
(152)
11.750
(298)
17.5
(445)
d.
B.
Acceptance Criteria:
1.
Average downflow velocity = 70 to 80 fpm (.35 to .41 m/s)
2.
Individual readings must be within +20 percent of the average downflow velocity.
Exhaust Air CFM/Access Opening Inflow Velocity (Applicable on all units)
a.
Instrument: Shortridge Flowhood ADM-870
b.
Procedure:
The inflow velocity is measured by using a Direct Inflow Measurement (DIM) Instrument (i.e.
shortridge flowhood). The DIM Instrument should be equipped with a flowhood that is as close
as possible to the width of the cabinet (i.e. NU-602-400 should use 1 x 4 foot flowhood). The
DIM Instrument should also be duct taped to the cabinet to prevent any sneak air paths from
occurring.
The DIM Instrument will read inflow volume (i.e. CFM). Use the window access opening area
and correction factor to calculate inflow velocity.
NuAire Spec
(fpm) Calculated
Model
NU-602400/E
NU-602500/E
NU-602600/E
DIM
(CFM)
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Access
Opening Area
DIM
CFM=FPM
Correction
Factor
Results
100-110
3.88
12"
371 = 96
96 + 9 (+9%) = 105 fpm
100-110
4.88
12”
490 = 100
100 + 5 (+5%) = 105 fpm
100-110
5.88
12"
555 = 94
94 + 11 (+12%) = 105 fpm
CFM
AREA
Correction
Factor
= FPM
39
Actual
FPM
C.
Alternate Procedure:
a.
Instrument: TSI 8355 Thermoanemometer.
b.
Procedure: Exhaust filter efflux is measured on a 4 x 4 inch (102mm) grid, 4 inches (102mm)
above the exhaust filter. The average access opening inflow velocity can be calculated from
these measurements, since the exhaust volume is equal to the inflow volume.
c.
Test Data - Inches (mm)
Back Wall
Number of Readings
3.25
(83)
7.25
(184)
11.25
(2.86)
ft./min(m/s)
Exhaust Area
ft.2(m2)
Calc. Exhaust
ft.3/min.(m3/s)
Volume
Access Opening
ft.2(m2)
Inflow Velocity
15.25
(387)
19.25
(489)
400/E
4.250
(108)
4.250
(108)
4.250
(108)
500/E
600/E
d.
e.
7.7
Ave. Exhaust.
Vel.
8.250
(210)
8.250
(210)
8.250
(210)
12.250
(311)
12.250
(311)
12.250
(311)
16.250
(413)
16.250
(413)
16.250
(413)
20.250
(514)
20.250
(514)
20.250
(514)
24.250
(616)
24.250
(616)
28.250
(718)
32.250
(819)
Acceptance Criteria:
1.
Access opening inflow velocity = 100 to 110 fpm (.51 to .56 m/s)
Meets Acceptance Criteria: Yes ______ No ______
Cabinet Leak Tightness Test
The cabinet leak tightness test can be performed by several different methods. However, to perform the
test, the window access area and the exhaust HEPA filter area must be sealed to pressurize the cabinet.
The window access area may be sealed by a custom plate or plastic and duct tape. The exhaust HEPA
filter area may also be sealed by a custom plate or plastic and duct tape. Sealing should be accomplished
on the cabinet face and cabinet top to assure a flat sealable surface.
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40
8.0
Error Indicators & Troubleshooting
Audible alarms and error indicators occur for a variety of reasons. Whenever an alarm condition is
present, the audible alarm and error indicator will be presented and stay on until the error is cleared. When
presented with an error indicator, please perform the following:
Step 1: NOTE ALL ERROR INDICATORS. When the cabinet is running, any and all red indicators
display an error.
Step 2: VERIFY ERROR INDICATORS. Error indicators can be verified by turning the errored
function on/off.
Step 3: MONITOR RE-OCCURRENCE OF ERROR INDICATORS. If re-occurrence of the error
indicator is immediate or daily, use guide below to correct the situation.
Error Indicator Troubleshooting Guide
Error Indicator
Indicator
Correction
Cabinet fluorescent lights
won't turn on.
Check blower/light circuit breaker on front of control
center. Check fluorescent lamps. Check voltage to
light ballast's. Check ballast. Check light switch.
Cabinet blower won't
turn on.
Check sliding window for correct operational height.
Check blower/light circuit breaker on front of control
center. Check voltage to blower. At motor voltage
regulator and at bulkhead connector. Check wiring to
blower. Check blower capacitor. Check sliding
window blower cutoff microswitch. Check blower
motor.
Cabinet outlets won't
turn on.
Check outlet circuit breaker on front of control center.
Check voltage to outlets.
Cabinet ultraviolet light
won't turn on.
Check blower/light circuit breaker on front of control
center. Check ultraviolet lamp. Check voltage to
ultraviolet ballast's. Check ballast. Check light switch.
Blower/lights circuit breaker
continues to trip after reset.
Check for short on output of circuit breaker. Replace
circuit breaker. Isolate output of circuit breaker by
disconnecting control center connectors, light circuit,
motor voltage regulator, etc. to isolate the short.
Magnehelic gauge
Magnehelic gauge
reads "NO" or
"LOW FLOW"
Check Magnehelic gauge operation. Check for
pinched tubing in control center. Make sure airflow is
not too low.
9.0
Remote Contacts
9.1
Exhaust System Contacts
The exhaust system contacts are single pole normally open contact closure outputs which are activated
whenever the blower switch is turned on. The contact points are located on the blower switch. Contact
ratings are 250 VAC maximum at 2 Amps.
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10.0 Optional Equipment
10.1
Ultraviolet Light
!
CAUTION: Ultraviolet light will injure your eyes. Avoid direct viewing at all times.
Personnel should not be present when ultraviolet lamp is on
10.1.1 Overview
The germicidal ultraviolet is primarily intended for the destruction of bacteria and other
microorganisms in the air or on directly exposed surfaces. Approximately 95% of the ultraviolet
radiations from germicidal tubes are in the 253.7 nanometer region. This is a region in the
ultraviolet spectrum which is near the peak of germicidal effectiveness. The exposure necessary
to kill bacteria is the product of time and intensity. High intensities for a short period of time, or
low intensities for a longer period are fundamentally equal in lethal dosage on bacteria
(disregarding the life cycle of bacteria). The intensity of light falling on a given area is governed
by the inverse law; that is the killing intensity decreases as the distance increases from the tube.
The germicidal tube is placed in the cabinet to provide an average intensity of 100
microwatts per square centimeter (for a new tube) falling on a horizontal plane defined by
the center of the work surface. The minimum requirement per paragraph 5.12 of NSF
Standard 49 is 40 microwatts per square centimeter (ref. NSF Std. #49, June, 1976).
10.1.2 Operation
The operation of the ultraviolet light is accomplished by pressing the UV switch location on the
front panel.
10.1.3 Precaution
The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and
reddening of the skin, if of sufficiently high intensity, or if exposure covers a prolonged period
of time. For this reason, one should avoid direct eye and skin exposure to ultraviolet light. If
exposure cannot be avoided, it is necessary for personnel to wear eye goggles or face shields,
and long sleeve gowns with rubber gloves.
Since the window of the NU-602 does not close completely, it is recommended that personnel
leave the cabinet face area when in use.
10.1.4 Maintenance
The output of an ultraviolet light deteriorates with burning age. The useful life of the light is
approximately 7000 hours under specific test conditions.
Before testing with lamp off, the light may be cleaned with a lint-free cloth dampened
 NOTE:
with alcohol or ammonia and water.
It is recommended that either a time schedule be established or the tube's output be measured
periodically and the tube replaced when its output falls below 40 microwatts per square
centimeter or exceeds 7000 hours of operation. Lights should be allowed to operate
approximately 5 to 10 minutes (longer when the light is in low temperatures) to warm up
sufficiently before reading the output with a meter.
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Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations (e)
Mold Spores
Penicillum roqueforti
Penicillium expansum
Penicillium digitiatum
Aspergillus glaucus
Aspergillus flavus
Aspergillus niger
Rhizopus nigricans
Mucor racemosus A
Mucor racemosus B
Oospora lactis
Microwatt
seconds
per cm/2
26,400
22,000
88,000
88,000
99,000
330,000
220,000
35,200
35,200
11,000
Protozoa
Microwatt
seconds
per cm/2
Paramecium
200,000(a)
Nematode Eggs
40,000(b)
Algae
22,000(c)
Virus
Baceriophage (E. Coli)
Tobacco Masaic
Influenze
6,600
440,000
3,400(d)
Yeasts
Saccharomyces
ellipsoideus
Saccharomyces cerevisiae
Brewers' yeast
Baker's yeast
Common yeast cake
13,200
17,600
13,200
6,600
8,800
13,200
Bacteria
Streptococcus lactis
Strep. hermolyticus (alpha type)
Staphylococcus aureus
Staphylococcus albus
Micrococcus sphaeroides
Sarcina lutea
Pseudomonas fluorescens
Escherichia coli
Proteus vulgaris
Serratia marcescens
Bacillus subtilis
Bacillus subtilis spores
Spirillum rubrum
8,800
5,500
6,600
5,720
15,400
26,400
7,040
7,040
7,480
6,160
11,000
22,000
6,160
References:
(a)
(b)
(c)
(d)
(e)
Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy,
D. Van Nostrand o., New York, New York, pp 253
Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162
Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp.,
pp. 713-714
Hollaender, A., Oliphant, J.W. (1944) The Inactivation Effect of Monochromatic Ultraviolet. Radiation
on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454
This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations" comes from
Westinghouse brochure entitled - "Westinghouse Sterilamp Germicidal Ultraviolet Tubes"
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11.0 Electrical/Environmental Requirements
11.1
Electrical: (Supply voltage fluctuations not to exceed +/- 10%)
*NU-602-4XX
*NU-602-5XX
*NU-602-6XX
**NU-602-4XXE
**NU-602-5XXE
**NU-602-6XXE
115 VAC,
115 VAC,
115 VAC,
230 VAC,
230 VAC,
230 VAC,
60 Hz,
60 Hz,
60 Hz,
50 Hz,
50 Hz,
50 Hz,
1 Phase,
1 Phase,
1 Phase,
1 Phase,
1 Phase,
1 Phase,
13 Amps
15 Amps
15 Amps
9 Amps
11 Amps
11 Amps
*UL/UL-C Listed **CE Certified

11.2
Operational Performance (for indoor use only)
Environment Temperature Range:
60°F - 90°F (15.6°C - 32.2°C)
Environment Humidity:
20% - 60% Relative Humidity
Environment Altitude:
6562 Feet (2000 Meters) Maximum
11.3
Light Exposure
Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity.
11.4
Installation Category: 2.0
Installation category (overvoltage category) defines the level of transient overvoltage, which the
instrument is designed to withstand safely. It depends on the nature of the electricity supply and its
overvoltage protection means. For example, in CAT II, which is the category used for instruments in
installations supplied from a supply comparable to public means, such as hospital and research
laboratories and most industrial laboratories, the expected transient overvoltage is 2500 V for a 230 V
supply and 1500 V for a 120 V supply.
11.5
Pollution Degree:
2.0
Pollution degree describes the amount of conductive pollution present in the operating environment.
Pollution degree 2 assumes that normally only non-conductive pollution such as dust occurs with the
exception of occasional conductivity caused by condensation.
11.6
Chemical Exposure
Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting.
Chlorinated and Halogen materials are not recommended for use on stainless steel surfaces. Chamber
decontamination can be accomplished by paraformaldehyde, vapor phased Hydrogen Peroxide or
Ethylene Oxide without degradation of cabinet materials.
11.7
EMC Performance (classified for light industrial)
Emissions:
EN61326
Immunity:
EN61326
NOTE: The EMC performance requirements are generated within the product enclosure. The enclosure will be
all metal grounded to earth. In addition, the membrane front panel will also include a ground plane for
maximum protection and an electrostatic shield.
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12.0 Disposal and Recycle
Cabinets that are no longer in use and are ready for disposal contain reusable materials. ALL
components with the exception of the HEPA filters may be disposed and/or recycled after they are
known to be properly disinfected.
 NOTE:
Follow all local, state and federal guidelines for disposal of HEPA filter solid waste.
BIOHAZARD
!
CAUTION
Prior to any disassembly for disposal, the cabinet must be
decontaminated.
RECYCLE
Component
Main Cabinet (Top)
Main Cabinet (Bottom)
Front Grill
Worksurface
Window
Window Frame
Front Service Panel
Control Center
Supply Diffuser
Exhaust Filter
HEPA Filter Frames
Hepex Bag
Blower Wheel & Housing
Motor
Printed Wiring Assembly
Wire
Ballasts
Connectors
Hardware
Lift Motor
Castors
Lift System Base Plate
Lift System Glides
LEAD FREE
Material
Stainless Steel
Painted Steel
Stainless Steel
Stainless Steel
Safety Glass
Stainless Steel
Painted Steel
Painted Steel
Aluminum
Aluminum
Painted Steel
PVC
Steel
Various Steel/Copper
Lead Free Electronic
PVC Coated Copper
Various Steel, Electronic
Nylon
Stainless Steel and Steel
Various Steel /Copper
Stainless Steel
Stainless Steel
Plated Steel
NOTE: Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic.
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