Introduction. Thermo Fisher Scientific Pierce Pre-Coated Iodination Tubes
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INSTRUCTIONS
Pierce Pre-Coated Iodination Tubes
28601
Number
28601
MAN0016379
Rev. A.0
Pub. Part No. 2160692.5
Description
Pierce Pre-Coated Iodination Tubes, 10 borosilicate test tubes (12 75mm) coated with 50µg Pierce
Iodination Reagent (formerly called “IODO-GEN” Iodination Reagent)
Tube-to-tube variation: 5%
Storage: Upon receipt store at room temperature protected from moisture. After opening barrier pouch, close the interior resealable bag containing the tubes with the yellow closures and desiccant.
Table of Contents
Introduction ................................................................................................................................................................................. 1
Important Product Information .................................................................................................................................................... 2
Example Protocol I: The Chizzonite Indirect Method for Iodination .......................................................................................... 2
Example Protocol II: Direct Method for Iodination ..................................................................................................................... 3
Example Protocol III: Iodination of Crosslinkers ........................................................................................................................ 4
Cited References .......................................................................................................................................................................... 5
General References ...................................................................................................................................................................... 5
Introduction
The Thermo Scientific™ Pierce™ Pre-Coated Iodination Tubes are a ready-to-use source of Pierce Iodination Reagent that provides convenient, consistent radioiodination of proteins and cell membranes.
1 Pierce Iodination Reagent (1,3,4,6tetrachloro-3 , 6 -diphenylglycouril; formerly called “IODO-GEN” Iodination Reagent) is an effective and mild oxidation reagent. This reagent is as effective as enzymatic methods for iodination of externally exposed residues and as effective as chloramine-T for general protein iodination, in addition to being more mild retained cell viability.
2
1-5 resulting in limited oxidative damage and
During oxidative reactions in the Pre-Coated Tubes, which are coated at the bottom with about 100µL, the iodination reagent remains adherent to the vessel because it is insoluble in typical aqueous media. This feature enables the sample to be decanted from the coated vessel to terminate the oxidative reaction. Although Pierce Iodination Reagent can be coated to any borosilicate glass surface, coating and drying procedure is very difficult to achieve in the typical laboratory without the reagent becoming inactive or flaking from the surface. The ability of the reagent to adhere tightly to a vessel depends on temperature, humidity, solvent and evaporation rate, and the coating procedure can be time-consuming, troublesome and difficult to reproduce. We have optimized these conditions in preparing the Pre-Coated Iodination Tubes, providing a readyto-use product for convenient iodination of typical protein and peptide samples.
Pierce Pre-Coated Iodination Tubes eliminate the troublesome coating step and offers a tightly adherent film of iodination reagent that will not flake off the tube walls. Each tube is consistently coated and exhibits less than 5% variation from tubeto-tube. The consistent coating results in highly reproducible radioiodinations.
Pierce Biotechnology
3747 N. Meridian Road
PO Box 117
Rockford, lL 61105 USA
(815) 968-0747
(815) 968-7316 fax
Thermofisher.com

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