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C
HAPTER
F
OURTEEN
: M
OLECULAR
W
EIGHT
C
ALIBRATION
3. In the new window, select the standard you wish to copy. Then click on Copy. on Add to enter a new Value or click on a value and click Edit to edit what you wish in the copy. Then click on OK.
A
PPLYING A
S
TANDARD TO A
L
ANE
Calibrating a Lane
1. Select the lane you wish to calibrate.
Master Tools > Molecular Weight, Step
1: Select MW standard tab window will appear. From the list of standards, choose the standard you wish to use. If the standard is not on the list, add the standard to the list (see Managing Weight
Standards). Click Next. A new screen appears Step 2: Calibrate MW standard. weights to match the bands that actually appear. Complete instructions for this appear below.
You also can access this feature using the 1D Analysis > Rf lines tool plugin.
Using the Stretch Factor
The stretch factor establishes a mathematical relationship between the weights to describe their relative movement. The larger the stretch factor, the lighter weights move in relationship to heavy ones. The smaller the stretch factor, the less the lighter weights move. A stretch factor of 1.0 indicates linear movement (weights move in direct proportion to their relative weights).
To adjust the weights to match the bands, you may choose to use the stretch factor: calibration mode is selected.
2. Drag a known weight up or down with the mouse until it matches the appropriate band. Alternatively, you can select the weight with the mouse (or TAB key) and move the weight up and down with the UP and DOWN arrows on the keyboard
(this helps adjust the weight by small amounts). When you have a match of the weight with the band, the line color changes from blue to yellow (default colors).
3. Adjust the stretch factor (scaling) between weights until the other weights match their appropriate bands. You can adjust it using the mouse wheel (rolling up increases the value, rolling down decreases it), or you can enter a new value into the text box and click Set.
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Table of contents
- 50 Ftp Transfer
- 51 Darkroom and Lens
- 52 Overview
- 52 Darkroom connectivity
- 54 Selecting a Camera
- 55 OptiChemi
- 60 BioChemi HR/Chemi HR
- 62 Fluor / Chemi Cameras
- 66 Digi Camera
- 70 Scanning Images
- 72 Purpose
- 72 How to Access the Player
- 72 Player Features
- 73 Player Options
- 74 Extract or Delete individual Image files (frames)
- 75 Create A Sequence (.sqv) file by Merging images
- 76 .AVI files
- 77 Loading Images
- 77 Saving Images
- 78 Image Files
- 79 Transferring Images Using FTP
- 81 Overview
- 81 Undo and Redo
- 82 Using Selection Tools
- 84 Paste
- 84 Paste Special
- 87 Overview
- 89 Rotate
- 90 Flip Horizontally
- 90 Flip Vertically
- 90 Resize
- 91 Reduce To Mono
- 91 Convert
- 92 Remove Noise
- 92 Despeckle
- 93 Sharpen
- 93 Emboss
- 94 Starfield Subtraction
- 95 Duplicating Images
- 95 Burn Changes on New Images
- 95 Background Correction
- 95 Background Subtraction
- 96 Overview
- 97 Viewing And Hiding Annotations
- 97 Types Of Annotation
- 98 Spatial Calibration
- 100 Rulers
- 100 Creating Annotations
- 101 The Text Annotation Window
- 102 Selecting Annotations
- 102 Moving And Resizing Annotations
- 103 Formatting Annotations
- 104 Deleting Annotations
- 105 Overview
- 106 Calibrating Image Scale
- 106 Image History
- 107 Understand a Pixel
- 109 Reports
- 110 Print
- 111 Overview
- 111 Navigation
- 114 Finding 1D Gel Lanes and Bands
- 117 Modifying Bands
- 119 Clearing All Lane and Band Information
- 120 Overview
- 120 Retardation factor (Rf) Lines
- 122 Molecular Weight Calibration
- 124 Applying a Standard to a Lane
- 125 Removing a Standard from a Lane
- 126 Overview
- 126 Background Correction
- 128 Concentration Calibration
- 130 Selecting Data Points
- 132 Selecting Curve Type
- 133 Removing Concentration Calibration
- 134 Overview
- 134 Lane Information
- 135 Band Information
- 136 Lane Profile Graph
- 139 Data Explorer
- 143 Fixed Image and Analysis Reports
- 146 Overview
- 146 Automatic Counting
- 147 Manual Colony Counting
- 151 Results Window and Explanation of Statistics
- 157 Purpose
- 157 Area Density Options
- 160 Steps to follow
- 164 Saving and Printing the Results and Data
- 164 Intensity Calibration Curves
- 169 Spatial Calibration
- 170 Purpose
- 170 Features
- 170 Usage
- 172 Secure User Accounts