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The software also allows you to choose the color of the graph's axes. To do so:
1. Underneath the Graph color options in Lane Profile Graph, click on the down arrow of Axis Color.
2. Select the color you wish to see. The software automatically changes the color.
Background Correction Options
If you wish to change the background correction option from the Lane Profile Graph:
1. Underneath the Background Correction options in Lane Profile Graph, click on the down arrow of No Background Correction.
2. Select the correction you wish to see. The software automatically changes the graph. You can also select to see a graph that takes into account the corrected values.
If you select Rolling Disc, a new dialog box appears asking you to enter the desired radius size of the disc.
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Viewing Data Explorer Tabular Results
Aside from viewing graphs and information windows about the lanes and bands, the software also offers you the option of seeing the data in a spreadsheet format that is user configurable.
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To access this Analysis > 1D Analysis > Master Tools, select Data Explorer.
Data Explorer opens a tabular format with the ability to include or exclude various data fields from the Data Explorer Report. Predefined report configurations are included to quickly select/deselect data fields appropriate to certain experiments.
The Data Explorer window also offers Report Printing and Data Export options.
The top left corner of the Data Explorer window offers a drop-down menu for quickly selected preconfigured reports rather than having to manually filter report data for commonly reported analysis data. When selecting theses reports you will notice the various fields being selected/deselected from the list of data fields.
You also can access this feature using the 1D Analysis Plugin.
Filtering Data
Accessible when you create tabular reports or export data, the drop-down menu allows you to choose what specific data you would like to appear in reports and in files you export.
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To Select Filter Data Fields
The top left corner of the Data Explorer window offers a drop-down menu for quickly selected preconfigured reports rather than having to manually filter report data for commonly reported analysis data.
When selecting theses reports you will notice the various fields being selected/deselected from the list of data fields. Select from:
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Image Name
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Number of lanes
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Background information
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Disk radius
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Lane and band information
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Concentrations
•
Molecular weights
To Show Filtered Data
To the right of the data fields is the actual analysis data which includes the fields currently selected. Next to the image name there is a + symbol which indicates all of the analysis data is minimized under the image name.
1. To show the analysis data, click the + symbol.
2. To further expand the data views for the individual categories, select the + symbol to the left of each category.
3. If you wish to filter the data further, select/deselect the appropriate data fields on the left side of the Data Explorer window.
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Any data column can be removed from the report by clicking and dragging that data column back to the data field on the left side.
Printing Data Explorer Tabular Reports
In Tabular Reports, you can print whatever data you select about the image and the analyses you have performed on the bands and lanes.
To Print Data Explorer Tabular Reports
1.
In the bottom left corner of the Data Explorer Tabular Reports window there are several print options: a. Page header: Displays as the page title on the top of the report b. Page footer: Displays as the page information at the bottom of the report c. Page setup: Displays the page setup options as offered by your specific printer d. Print preview: Displays a preview of what will be print on the report.
2.
Enter the header and footer information, set the page format, and click Print.
When the print window opens, click OK.
Export Data Explorer Reports
LS software allows you to export data to Microsoft Excel® or to other software packages for further analysis or documentation.
To Export Data Explorer Tabular Reports
To export data:
1.
Under Analysis > 1D Analysis >
Master Tools, select Data Explorer.
The Tabular Reports window appears.
2.
Select the data fields you wish to export.
3.
In the bottom right corner of the Data
Explorer Tabular window, you can select from two options and select whether you wish to export the data by
Comma, Semicolon, Space, Tab
(where you type in the delimiting character or characters):
•
To Excel
•
To CSV
4.
Click the To Excel or To CSV button.
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Table of contents
- 50 Ftp Transfer
- 51 Darkroom and Lens
- 52 Overview
- 52 Darkroom connectivity
- 54 Selecting a Camera
- 55 OptiChemi
- 60 BioChemi HR/Chemi HR
- 62 Fluor / Chemi Cameras
- 66 Digi Camera
- 70 Scanning Images
- 72 Purpose
- 72 How to Access the Player
- 72 Player Features
- 73 Player Options
- 74 Extract or Delete individual Image files (frames)
- 75 Create A Sequence (.sqv) file by Merging images
- 76 .AVI files
- 77 Loading Images
- 77 Saving Images
- 78 Image Files
- 79 Transferring Images Using FTP
- 81 Overview
- 81 Undo and Redo
- 82 Using Selection Tools
- 84 Paste
- 84 Paste Special
- 87 Overview
- 89 Rotate
- 90 Flip Horizontally
- 90 Flip Vertically
- 90 Resize
- 91 Reduce To Mono
- 91 Convert
- 92 Remove Noise
- 92 Despeckle
- 93 Sharpen
- 93 Emboss
- 94 Starfield Subtraction
- 95 Duplicating Images
- 95 Burn Changes on New Images
- 95 Background Correction
- 95 Background Subtraction
- 96 Overview
- 97 Viewing And Hiding Annotations
- 97 Types Of Annotation
- 98 Spatial Calibration
- 100 Rulers
- 100 Creating Annotations
- 101 The Text Annotation Window
- 102 Selecting Annotations
- 102 Moving And Resizing Annotations
- 103 Formatting Annotations
- 104 Deleting Annotations
- 105 Overview
- 106 Calibrating Image Scale
- 106 Image History
- 107 Understand a Pixel
- 109 Reports
- 110 Print
- 111 Overview
- 111 Navigation
- 114 Finding 1D Gel Lanes and Bands
- 117 Modifying Bands
- 119 Clearing All Lane and Band Information
- 120 Overview
- 120 Retardation factor (Rf) Lines
- 122 Molecular Weight Calibration
- 124 Applying a Standard to a Lane
- 125 Removing a Standard from a Lane
- 126 Overview
- 126 Background Correction
- 128 Concentration Calibration
- 130 Selecting Data Points
- 132 Selecting Curve Type
- 133 Removing Concentration Calibration
- 134 Overview
- 134 Lane Information
- 135 Band Information
- 136 Lane Profile Graph
- 139 Data Explorer
- 143 Fixed Image and Analysis Reports
- 146 Overview
- 146 Automatic Counting
- 147 Manual Colony Counting
- 151 Results Window and Explanation of Statistics
- 157 Purpose
- 157 Area Density Options
- 160 Steps to follow
- 164 Saving and Printing the Results and Data
- 164 Intensity Calibration Curves
- 169 Spatial Calibration
- 170 Purpose
- 170 Features
- 170 Usage
- 172 Secure User Accounts