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4
Routine Maintenance
Replacing an Inline Filter for the EASY-nLC 1000 Instrument
6. Do one of the following:
• For a solvent-side check valve, reconnect the solvent inlet line.
• For a waste-side check valve, reconnect the waste line.
7. Mount the right side panel to the instrument housing. Then, with a #2 Phillips head screwdriver, secure the panel by rotating the three captive screws a quarter-turn clockwise.
8. Run the Purge Solvent script for the pump associated with the replaced check valve, and monitor the flow from the solvent bottle when the pump is filling and the flow to the waste beaker when the pump is emptying (see
“Prepare – Purge Solvent” on page 32 ).
Replacing an Inline Filter for the EASY-nLC 1000 Instrument
Inline filters are connected to both the inlet and outlet ports of the flow sensors. The inline filter consists of a stainless steel body (nut, filter, and tube) and a ferrule (see
Figure 69. Inline filter components (nut with integrated tube and filter and ferrule)
Thermo Scientific
Replacing an inline filter requires the following tools and materials.
Tools
• 5/16 in. open-ended wrench
• #2 Phillips head screwdriver
Parts and materials
• Powder-free safety gloves
• Inline filter
CAUTION Wear powder-free gloves and safety glasses when handling parts of the LC system that come into contact with solvents.
To install an inline filter into the receiving port of a flow sensor
1. Close down the EASY-nLC system, and then turn off the power to the instrument
(see
“Closing Down the EASY-nLC Instrument” on page 21 ).
2. Using a #2 Phillips head screwdriver, with a quarter-turn loosen the three captive screws that secure the right side panel to the instrument housing. Then remove the panel.
EASY-nLC Series Troubleshooting and Maintenance Guide
81
4
Routine Maintenance
Replacing an Inline Filter for the EASY-nLC 1000 Instrument
3. While holding the wide end of the ferrule toward the nut, slide the ferrule over the tube that extends from the threaded end of the nut.
IMPORTANT The inline filters have a swept volume (flow path volume) of less than one microliter.
To prevent leaks, take care to properly seat the ferrule in the receiving port. Once the ferrule is swaged onto the tubing, do not connect the fitting to a different receiving port.
4. Using a 5/16 in. open-ended wrench, tighten the nut.
5. Install the nanoViper solvent inlet line into the inline filter (see
Figure 70. Installed inline filters
Inline filter
6. Run the Back Pressure script and the Leak script on the system as described in these topics:
•
“Test – Back Pressure” on page 48
•
82
EASY-nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
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Table of contents
- 13 Contents
- 19 Preface
- 20 Related Documentation
- 20 Downloading Manuals from the Customer Manuals Web Site
- 21 Safety and Special Notices
- 23 Contacting Us
- 25 Introduction
- 26 Hardware Components
- 26 Autosampler
- 27 ASC Model—Current
- 28 ASA Model—Legacy EASY-nLC II Instruments
- 29 Solvent System Components Behind the Right Side Panel
- 29 View Behind the Right Side Panel
- 32 Syringe Pumps
- 34 Pressure Sensors
- 34 Flow Sensors
- 35 Check Valve Assemblies
- 36 Six-Port Rotary Valves
- 38 Autosampler Needle
- 39 Computer and Autosampler Behind the Left Side Panel
- 40 Back Panel
- 42 Touch-Screen Application
- 42 Description of the Touch-Screen Monitor
- 44 Logging In to the EASY-nLC Instrument for Maintenance Tasks
- 45 Closing Down the EASY-nLC Instrument
- 47 Specifications
- 47 Performance Specifications
- 48 Technical Specifications
- 51 Maintenance Schedule
- 51 Daily Maintenance
- 52 Weekly Maintenance
- 52 Quarterly Maintenance
- 53 Yearly Maintenance
- 53 Field Service Repairs
- 55 Maintenance Scripts and Service Records
- 55 Maintenance Scripts
- 56 Prepare – Purge Solvent
- 57 Prepare – Flush Air
- 58 Prepare – Precolumn Equilibration
- 60 Prepare – Analytical Column Equilibration
- 62 Prepare – Isocratic Flow
- 63 Test – MS Connection
- 66 Test – Sample Pickup
- 68 Test – Leaks
- 71 Test – Valve Check
- 72 Test – Back Pressure
- 73 Test – Autosampler Torque
- 74 Test – Pump Torque
- 74 Calibrate – Valve Tune
- 75 Calibrate – Flow Sensors
- 76 Calibrate – Reset Pressure Sensor
- 77 Calibrate – Direct Infusion
- 79 Keeping Service Records
- 80 Checking and Resetting the Device Usage Counters
- 81 Routine Maintenance
- 82 Maintaining a Clean Working Environment
- 82 Replacing the Main Power Fuse
- 84 Maintaining the Syringe Pumps
- 85 Retracting the Piston
- 86 Replacing the Piston Seal and Cleaning the Piston in a PLF Pump
- 91 Replacing the Piston Seal in a PLU Pump
- 95 Priming the Pump
- 96 Resetting the Pump Usage Counter
- 97 Removing Air After Replacing a Piston Seal or a Pump
- 97 Running the Leaks Script after Replacing a Piston Seal or a Pump
- 98 Maintaining the Rotary Valves
- 98 Cleaning the Rotor Seal and Stator
- 101 Replacing the Rotor Seal
- 102 Replacing the Stator
- 104 Replacing the Check Valves
- 105 Replacing an Inline Filter for the EASY-nLC 1000 Instrument
- 107 Using nanoViper Fittings
- 108 Replacing the Autosampler Needle
- 113 Replacing the Sample Loop
- 114 Replacing a Pressure Sensor for the PLU Pump
- 116 Replacing a Flow Sensor
- 116 Replacing a Flow Sensor in the EASY-nLC 1000 Instrument
- 120 Replacing a Flow Sensor in the EASY-nLC II Instrument
- 124 Replacing the Hard Drive
- 129 Managing the Devices List
- 131 Field Service Maintenance
- 132 Replacing the Built-In Computer
- 137 Replacing the Monitor
- 141 Replacing a Pressure Sensor for a PLF Pump
- 146 Replacing the ASC Autosampler
- 152 Upgrading from an ASA Autosampler to an ASC Autosampler
- 161 Replacing the Autosampler Cooler
- 161 Replacing the ASC Cooler
- 164 Replacing the ASA Cooler
- 171 Replacing a Rotary Valve
- 174 Replacing a Pump
- 174 Replacing a PLU Pump
- 178 Replacing a PLF Pump with a PLU Pump
- 187 Replacing a Pump PCB
- 187 Replacing a Pump PCB in the PLU Pump
- 191 Replacing a Pump PCB in the PLF Pump
- 193 Troubleshooting
- 194 Troubleshooting Tips
- 195 Autosampler Problems
- 196 Contact Closure Problems
- 196 Instrument Startup Problems
- 197 Delayed Elution
- 198 Leaks that Cause Delayed Elution
- 200 Introduced Swept Volume
- 202 Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps
- 204 System Reaches Its Maximum Pressure During the Gradient
- 207 Sample Signal Weak or Absent
- 208 Weak or Absent Signal—Sample Not Aspirated
- 209 Weak or Absent Signal—Defective Precolumn
- 210 Weak or Absent Signal—Leak Upstream of Flow Sensor B
- 211 Slow or No Pressure Increase in Subsystem A or B
- 213 Errors Reported by the Xcalibur Data System
- 218 Carryover
- 220 Spray Issues
- 221 Chromatographic Performance
- 222 Device Failures
- 226 Miscellaneous
- 228 Using the Direct Controls for Troubleshooting and Maintenance
- 228 Using the Valve Controls
- 229 Using the Pump Controls
- 229 Opening the Direct Controls for the Pumps
- 230 Using the Direct Controls To Fill or Empty Pump A or B
- 233 Using the Direct Controls To Fill or Empty Pump S
- 234 Using the Autosampler Controls
- 236 Troubleshooting a Pump that Fails the Flush Air Script
- 240 Troubleshooting a Pump that Fails the Leaks Script
- 242 Running a System Leak Test
- 245 Troubleshooting the Results of the System Leak Test
- 246 Checking Valve S for a Leak
- 246 Checking the Sample Loop Connections for a Leak
- 247 Locating a Leak in the Solvent Path from Valve B to Flow Sensor B
- 248 Locating a Leak in the Solvent Path from Pump A to Flow Sensor A
- 250 Locating a Leak In or Between Valve S and Valve W
- 258 Locating a Leak Between the Flow Sensors and Valve S
- 259 EASY-nLC 1000: Locating a Leak Between the Flow Sensors and Valve S
- 270 EASY-nLC II: Locating a Leak Between the Flow Sensors and Valve S
- 272 Identifying a Leaking Check Valve
- 273 Troubleshooting a System Blockage
- 274 Running the Back Pressure Test for the A and B Solvent Paths
- 275 Troubleshooting a System Blockage when Test Solvent A Fails
- 281 Troubleshooting a System Blockage when Test Solvent B Fails
- 285 Troubleshooting the Autosampler Aspiration and Calibration
- 287 Checking Sample Pickup
- 288 Troubleshooting Communication Problems
- 288 Network Access
- 289 Network Connection Failures
- 290 Testing the Network Connection to the EASY-nLC Computer
- 292 Verifying that the LC/MS System Is Properly Grounded
- 293 Calibrating the Autosampler’s XYZ Robot
- 294 Replacing the Autosampler Adapter Plate
- 295 Replacing the Adapter Plate in the ASC Model Autosampler
- 296 Replacing the Adapter Plate in the ASA Model Autosampler
- 298 Managing Plate Formats
- 298 Selecting a Plate Format
- 299 Creating a New Plate Format
- 301 Deleting a Plate Format
- 302 Preparing the Autosampler for the Calibration Routines
- 304 Calibrating Plates
- 309 Calibrating the Wash Bottle and Extra Vial Positions
- 311 Remote Support
- 312 Connecting the EASY-nLC Instrument to the Support Server
- 317 Saving System Files on a USB Removable Storage Device
- 320 Downloading the Latest Firmware File
- 323 Returning the EASY-nLC Instrument
- 323 Transport Instructions
- 325 Declaration of Contamination
- 327 Error Codes
- 335 Mobile Phase Viscosity
- 337 Consumables and Replacement Parts
- 337 Ordering Information
- 338 Solvent System Schematic for the EASY-nLC II Instrument
- 340 Solvent System Schematic for the EASY-nLC 1000 Instrument
- 342 Common Replacement Parts
- 342 Common Replacement Parts for the EASY-nLC II Instrument
- 343 Common Replacement Parts for the EASY-nLC 1000 Instrument
- 345 Quick Reference Guides for Routine Maintenance
- 347 Using nanoViper Fittings Quick Reference Guide
- 347 Connecting nanoViper Fittings
- 348 nanoViper Tubing Connections
- 349 Returning the System to Atmospheric Pressure
- 349 Upstream of Valve S Solvent Lines
- 350 Downstream of Valve S Solvent Lines
- 351 Maintaining the PLU Pump Quick Reference Guide
- 353 Maintaining the PLF Pump Quick Reference Guide
- 355 Maintaining the Rotary Valves Quick Reference Guide
- 357 Replacing a Damaged Autosampler Needle
- 359 Index