EASY-nLC Series Xcalibur Data System 2.1 or Later User Guide

EASY-nLC Series Xcalibur Data System 2.1 or Later User Guide

P

Preface

This guide describes how to configure the Thermo EASY-nLC™ virtual instrument (VI) control software from the Thermo Foundation™ platform, set up the liquid chromatography

(LC) parameters for LC/MS instrument methods in the Instrument Setup window of the

Thermo Xcalibur™ data system, and set up the sample position settings and injection volumes for an injection sequence from the Sequence Setup view in the Xcalibur data system.

IMPORTANT To establish communication with the EASY-nLC instrument and to avoid the premature termination of data acquisition during sequence runs, set up the data system computer as described in

“Data System Computer Settings” on page viii

.

Contents

Related Documentation

System Requirements

Data System Computer Settings

Cautions and Special Notices

Contacting Us

To suggest changes to the documentation or to the Help

Complete a brief survey about this document by clicking the button below.

Thank you in advance for your help.

Thermo Scientific EASY-nLC Series User Guide for the Xcalibur Data System

v

Preface

Related Documentation

Thermo Fisher Scientific provides these manuals as PDF files on the data system computer and on the USB flash drive that ships with the EASY-nLC instrument:

• EASY-nLC Series Preinstallation Requirements Guide

EASY-nLC Series Getting Started Guide (using the stand-alone, touch-screen application)

• EASY-nLC Series Troubleshooting and Maintenance Guide

• EASY-nLC Series User Guide for the Xcalibur Data System

The data system also provides Help for the EASY-nLC virtual instrument control software.

To access the EASY-nLC manuals on the data system computer

Choose Start > Programs (or All Programs) > Thermo Instruments > Manuals >

LC Devices > Thermo.

To open the Help from the EASY-nLC view of the Instrument Setup window

• To open the Welcome page of the Thermo EASY-nLC Help, choose Help > Thermo

EASY-nLC Help from the menu bar.

• To open the Help topic for the current page of the Thermo EASY-nLC view, do one of the following:

– Choose Help > Help On Current Item F1 from the menu bar.

– Press the F1 key on the computer keyboard.

To open the Help from the Thermo EASY-nLC Configuration dialog box of the

Foundation Instrument Configuration window

Press the F1 key on the computer keyboard.

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EASY-nLC Series User Guide for the Xcalibur Data System Thermo Scientific

Preface

System Requirements

Table 1 lists the data system computer hardware and software requirements for the EASY-nLC

device driver. This device driver is provided on the LC Devices DVD and is designed for use with the Thermo Xcalibur data system or an equivalent Thermo Scientific mass spectrometry application.

IMPORTANT Before you install the device driver provided on the LC Devices DVD, ensure that the data system computer has a compatible version of the Foundation platform as noted in the Thermo LC Devices x.x.x Release Notes, where x.x.x is the version you want to install. You can find the Release Notes document, which includes the system requirements, on the LC Devices DVD.

Table 1. Data system computer hardware and software requirements

Hardware

Data system computer

Minimum requirements

• 2 GHz processor with 1 GB RAM

• DVD-ROM drive

• 80 GB hard drive capacity

• Display resolution 1280 × 1024 resolution

• 2 Network cards

• NTFS format

Requirements Software

Operating system

Third-party software

Thermo Scientific software

Microsoft™ Windows™ operating system:

• Windows 7 Professional (32-bit and 64-bit a

)

–or–

• Windows XP Workstation SP3

Adobe™ Reader™ 9.0 or later

LC Devices

EASY-nLC device driver

Foundation platform

2.8.0 or later 1.0.2 SP2 or later

2.7.0

1.0.2 SP2 or 2.0.0 SP1 a

LC Devices 2.8.0 and later are compatible with Windows 7, 32-bit and 64-bit

Thermo Scientific EASY-nLC Series User Guide for the Xcalibur Data System

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Preface

Data System Computer Settings

Table 2 lists the computer settings that are required to avoid the premature termination of

data acquisition during sequence runs. It also provides the virus scanner setting that is required to establish communication with the EASY-nLC instrument.

IMPORTANT After you install the Thermo EASY-nLC device driver on a Windows 7 computer, exclude the Thermo EASY-nLC software from the Prevent IRC

Communication list for the computer’s virus scanner (see

“Excluding the EASY-nLC from the VirusScan Enterprise” on page 5

).

If you do not exclude the Thermo EASY-nLC software from the Prevent IRC

Communication list, the data system computer cannot establish communication with the

EASY-nLC instrument.

Table 2. Data system computer settings

Category

Power saver

Settings

• Screen saver: None

• Hibernate: Not enabled or Never

• Turn off hard disk: Never

Windows updates

Virus scanner

• Turn off monitor: Never

If the data system computer is connected to the Internet, turn off the automatic Windows updates.

For the Windows 7 operating system, exclude the

Thermo EASY-nLC software from the virus scanner.

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EASY-nLC Series User Guide for the Xcalibur Data System Thermo Scientific

Preface

Cautions and Special Notices

Make sure you follow the cautions and special notices presented in this guide. Cautions and special notices appear in boxes; those concerning safety or possible system damage also have corresponding caution symbols.

This guide uses the following types of cautions and special notices.

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of data, or invalid test results; or might contain information that is critical for optimal performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Thermo Scientific EASY-nLC Series User Guide for the Xcalibur Data System

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Preface

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

To contact Technical Support for the EASY-nLC instrument

Phone

Fax

Web site

E-mail

800-532-4752

561-688-8736 www.proxeon.com

(North and South America) [email protected]

(Other continents) [email protected]

Knowledge base www.thermokb.com

Find software updates and utilities to download at mssupport.thermo.com

.

To order consumable and spare parts for the EASY-nLC instrument

For the EASY-nLC 1000 instrument, go to www.proxeon.com/productrange/nano_lc_easy-nlc_1000/accessories_spares/index.html

.

For the EASY-nLC II instrument, go to www.proxeon.com/productrange/nano_lc/accessories-spares/index.html

.

To get local contact information for sales or service

Go to www.thermoscientific.com/wps/portal/ts/contactus .

To copy manuals from the Internet

Go to mssupport.thermo.com

, agree to the Terms and Conditions, and then click

Customer Manuals in the left margin of the window.

To suggest changes to the documentation or to the Help

• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62 .

• Send an e-mail message to the Technical Publications Editor at [email protected]

.

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EASY-nLC Series User Guide for the Xcalibur Data System Thermo Scientific

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