Replacing a Pump PCB. Thermo Fisher Scientific EASY-nLC Series Troubleshooting and
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5
Field Service Maintenance
Replacing a Pump PCB
Replacing a Pump PCB
The procedure for replacing the pump PCB differs between the PLU model pump and the
PLF model pump.
Note The PLU pump is standard in the EASY-nLC 1000 instrument. The PLF pump is standard in most EASY-nLC II instruments.
Figure 8 on page 9 shows these two pump
models.
To replace a pump PCB, follow the appropriate procedure:
•
“Replacing a Pump PCB in the PLU Pump,” on this page
•
“Replacing a Pump PCB in the PLF Pump” on page 167
Replacing a Pump PCB in the PLU Pump
Replacing the pump PCB on the PLU model pump requires these items.
Tools
• #2 Phillips screwdriver
• 2.5 mm hex wrench
• Small flathead screwdriver
Parts
Pump PCB, P/N LC574
To replace the pump PCB on the PLU pump, follow these steps:
1.
“To remove a pump PCB from the PLU model pump,”
on this page
2.
“To replace a pump PCB on the PLU model pump” on page 166
To remove a pump PCB from the PLU model pump
1. Close down the EASY-nLC 1000 instrument as described in
EASY-nLC Instrument” on page 21 .
2. Disconnect the power cable from the instrument’s back panel.
3. Using a #2 Phillips head screwdriver, with a quarter-turn loosen the three captive screws that secure the right side panel to the instrument housing. Then remove the panel.
4. Disconnect the P-Bus communication cables, the flow sensor cable, and the pressure sensor cable from the pump.
Figure 176 on page 164 shows these cables connected to the pump.
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5
Field Service Maintenance
Replacing a Pump PCB
5. Using a 2.5 mm L-hex wrench or ball driver, loosen the four captive screws on the PCB shield (see
Figure 176 ). Then remove the PCB shield (see Figure 177
).
Figure 176. Four PCB shield screws (PLU model pump)
Flow sensor cable
Pressure sensor cable
P-Bus cables
The four captive screws remain in place (see Figure 177
).
Figure 177. Removing the PCB shield (PLU model pump)
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EASY-nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
5
Field Service Maintenance
Replacing a Pump PCB
6. Slide the PCB forward and away from the four mounting studs.
7. Disconnect the motor cable on the back of the pump PCB (see
Figure 178. Motor cable connected to the pump PCB (PLU model pump)
Motor cable
Thermo Scientific EASY-nLC Series Troubleshooting and Maintenance Guide
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Field Service Maintenance
Replacing a Pump PCB
To replace a pump PCB on the PLU model pump
1. Using a small flathead screwdriver, reset the address on the rotary switch on the pump
) as follows:
• Pump A =1
• Pump B = 2
• Pump S = 3
Figure 179. PCB rotary switch
PCB rotary switch
2. Plug the motor cable into the back of the PCB (see Figure 178 on page 165 ).
3. Insert the new pump PCB onto the pump.
4. Install the PCB shield.
5. Using a 2.5 mm L-hex wrench or ball driver, tighten the four 2.5 mm hex screws in the
PCB shield.
6. Reconnect the P-Bus communication cables, the flow sensor cable, and the pressure
sensor cable (see Figure 176 on page 164
) to the pump.
7. Mount the right side panel to the instrument housing. Then, with a #2 screwdriver, secure the panel by rotating the three captive screws a quarter-turn clockwise.
8. Reconnect the power cable to the back panel of the instrument.
9. Turn on the instrument.
10. Wait for 30 minutes to allow the temperature in the housing to stabilize.
11. Calibrate the flow sensor by following the automated script described in “Calibrate –
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EASY-nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
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Field Service Maintenance
Replacing a Pump PCB
Replacing a Pump PCB in the PLF Pump
Replacing a pump PCB (PLF model pump) in the EASY-nLC II instrument requires these items.
Tools
• #2 Phillips screwdriver
• 2.5 mm hex wrench
• Small flathead screwdriver
Parts
Pump PCB, P/N LC274
To replace the PCB on a PLF pump, follow these steps:
1.
To remove the pump PCB from the PLF model pump (EASY-nLC II instrument)
2.
To install the pump PCB on a PLF pump (EASY-nLC II instrument)
To remove the pump PCB from the PLF model pump (EASY-nLC II instrument)
1. Close down the EASY-nLC II instrument as described in “Closing Down the EASY-nLC
.
2. Disconnect the power cable from the instrument’s back panel.
3. Using a #2 Phillips head screwdriver, with a quarter-turn loosen the three captive screws that secure the right side panel to the instrument housing. Then remove the panel.
4. Disconnect the P-Bus communication cables (see Figure 169 on page 158 ) from the
pump actuator.
5. Unscrew and remove the four 2.5 mm hex screws that connect the PCB to the pump.
6. Disconnect the pressure sensor grounding cable (yellow wire).
7. Gently slide the PCB outward and turn it around to gain access to its back side.
8. Disconnect the pressure sensor communication cable. Take care to avoid putting any stress on the cables.
9. Disconnect the motor cable. Again, take care to avoid putting any stress on the cables.
10. Remove the PCB.
11. Go to the next procedure
“To install the pump PCB on a PLF pump (EASY-nLC II instrument).”
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Field Service Maintenance
Replacing a Pump PCB
To install the pump PCB on a PLF pump (EASY-nLC II instrument)
1. Remove the damaged PCB as described in the previous procedure, “To remove the pump
PCB from the PLF model pump (EASY-nLC II instrument)” on page 167 .
2. Using a small flathead screwdriver, reset the address on the rotary switch on the new pump PCB as follows:
• Pump A =1
• Pump B=2
• Pump S=3
3. Reconnect the motor cable to the back of the PCB (see
).
4. Mount the new PCB onto the pump.
5. Secure the PCB to the pump as follows:
• Slide the four screws into the holes in the four corners of the PCB.
• Slide the connector at the end of the pressure sensor grounding cable under the screw in the lower-right corner of the PCB.
• Using a 2.5 mm hex wrench, tighten the four screws.
Figure 180. PLF pump with the pressure sensor cable connected
9
0 1
2
P-Bus cable
Pressure sensor grounding cable
P-Bus cable
Motor cable routed to the back of the PCB
CAUTION To avoid damaging the PCB, make sure that the grounding connector does not touch any other components.
6. Reconnect the P-Bus communication cables.
7. Calibrate the flow sensor by following the automated script described in “Calibrate –
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EASY-nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
6
Troubleshooting
To troubleshoot problems with the EASY-nLC instrument, follow the troubleshooting tips and procedures in this chapter.
Contents
•
•
Using the Direct Controls for Troubleshooting and Maintenance
•
Troubleshooting a Pump that Fails the Flush Air Script
•
Troubleshooting a Pump that Fails the Leaks Script
•
•
Troubleshooting the Results of the System Leak Test
•
Identifying a Leaking Check Valve
•
Troubleshooting a System Blockage
•
Troubleshooting the Autosampler Aspiration and Calibration
•
•
Troubleshooting Communication Problems
•
Verifying that the LC/MS System Is Properly Grounded
Thermo Scientific EASY-nLC Series Troubleshooting and Maintenance Guide
169
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Table of contents
- 13 Contents
- 19 Preface
- 20 Related Documentation
- 20 Downloading Manuals from the Customer Manuals Web Site
- 21 Safety and Special Notices
- 23 Contacting Us
- 25 Introduction
- 26 Hardware Components
- 26 Autosampler
- 27 ASC Model—Current
- 28 ASA Model—Legacy EASY-nLC II Instruments
- 29 Solvent System Components Behind the Right Side Panel
- 29 View Behind the Right Side Panel
- 32 Syringe Pumps
- 34 Pressure Sensors
- 34 Flow Sensors
- 35 Check Valve Assemblies
- 36 Six-Port Rotary Valves
- 38 Autosampler Needle
- 39 Computer and Autosampler Behind the Left Side Panel
- 40 Back Panel
- 42 Touch-Screen Application
- 42 Description of the Touch-Screen Monitor
- 44 Logging In to the EASY-nLC Instrument for Maintenance Tasks
- 45 Closing Down the EASY-nLC Instrument
- 47 Specifications
- 47 Performance Specifications
- 48 Technical Specifications
- 51 Maintenance Schedule
- 51 Daily Maintenance
- 52 Weekly Maintenance
- 52 Quarterly Maintenance
- 53 Yearly Maintenance
- 53 Field Service Repairs
- 55 Maintenance Scripts and Service Records
- 55 Maintenance Scripts
- 56 Prepare – Purge Solvent
- 57 Prepare – Flush Air
- 58 Prepare – Precolumn Equilibration
- 60 Prepare – Analytical Column Equilibration
- 62 Prepare – Isocratic Flow
- 63 Test – MS Connection
- 66 Test – Sample Pickup
- 68 Test – Leaks
- 71 Test – Valve Check
- 72 Test – Back Pressure
- 73 Test – Autosampler Torque
- 74 Test – Pump Torque
- 74 Calibrate – Valve Tune
- 75 Calibrate – Flow Sensors
- 76 Calibrate – Reset Pressure Sensor
- 77 Calibrate – Direct Infusion
- 79 Keeping Service Records
- 80 Checking and Resetting the Device Usage Counters
- 81 Routine Maintenance
- 82 Maintaining a Clean Working Environment
- 82 Replacing the Main Power Fuse
- 84 Maintaining the Syringe Pumps
- 85 Retracting the Piston
- 86 Replacing the Piston Seal and Cleaning the Piston in a PLF Pump
- 91 Replacing the Piston Seal in a PLU Pump
- 95 Priming the Pump
- 96 Resetting the Pump Usage Counter
- 97 Removing Air After Replacing a Piston Seal or a Pump
- 97 Running the Leaks Script after Replacing a Piston Seal or a Pump
- 98 Maintaining the Rotary Valves
- 98 Cleaning the Rotor Seal and Stator
- 101 Replacing the Rotor Seal
- 102 Replacing the Stator
- 104 Replacing the Check Valves
- 105 Replacing an Inline Filter for the EASY-nLC 1000 Instrument
- 107 Using nanoViper Fittings
- 108 Replacing the Autosampler Needle
- 113 Replacing the Sample Loop
- 114 Replacing a Pressure Sensor for the PLU Pump
- 116 Replacing a Flow Sensor
- 116 Replacing a Flow Sensor in the EASY-nLC 1000 Instrument
- 120 Replacing a Flow Sensor in the EASY-nLC II Instrument
- 124 Replacing the Hard Drive
- 129 Managing the Devices List
- 131 Field Service Maintenance
- 132 Replacing the Built-In Computer
- 137 Replacing the Monitor
- 141 Replacing a Pressure Sensor for a PLF Pump
- 146 Replacing the ASC Autosampler
- 152 Upgrading from an ASA Autosampler to an ASC Autosampler
- 161 Replacing the Autosampler Cooler
- 161 Replacing the ASC Cooler
- 164 Replacing the ASA Cooler
- 171 Replacing a Rotary Valve
- 174 Replacing a Pump
- 174 Replacing a PLU Pump
- 178 Replacing a PLF Pump with a PLU Pump
- 187 Replacing a Pump PCB
- 187 Replacing a Pump PCB in the PLU Pump
- 191 Replacing a Pump PCB in the PLF Pump
- 193 Troubleshooting
- 194 Troubleshooting Tips
- 195 Autosampler Problems
- 196 Contact Closure Problems
- 196 Instrument Startup Problems
- 197 Delayed Elution
- 198 Leaks that Cause Delayed Elution
- 200 Introduced Swept Volume
- 202 Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps
- 204 System Reaches Its Maximum Pressure During the Gradient
- 207 Sample Signal Weak or Absent
- 208 Weak or Absent Signal—Sample Not Aspirated
- 209 Weak or Absent Signal—Defective Precolumn
- 210 Weak or Absent Signal—Leak Upstream of Flow Sensor B
- 211 Slow or No Pressure Increase in Subsystem A or B
- 213 Errors Reported by the Xcalibur Data System
- 218 Carryover
- 220 Spray Issues
- 221 Chromatographic Performance
- 222 Device Failures
- 226 Miscellaneous
- 228 Using the Direct Controls for Troubleshooting and Maintenance
- 228 Using the Valve Controls
- 229 Using the Pump Controls
- 229 Opening the Direct Controls for the Pumps
- 230 Using the Direct Controls To Fill or Empty Pump A or B
- 233 Using the Direct Controls To Fill or Empty Pump S
- 234 Using the Autosampler Controls
- 236 Troubleshooting a Pump that Fails the Flush Air Script
- 240 Troubleshooting a Pump that Fails the Leaks Script
- 242 Running a System Leak Test
- 245 Troubleshooting the Results of the System Leak Test
- 246 Checking Valve S for a Leak
- 246 Checking the Sample Loop Connections for a Leak
- 247 Locating a Leak in the Solvent Path from Valve B to Flow Sensor B
- 248 Locating a Leak in the Solvent Path from Pump A to Flow Sensor A
- 250 Locating a Leak In or Between Valve S and Valve W
- 258 Locating a Leak Between the Flow Sensors and Valve S
- 259 EASY-nLC 1000: Locating a Leak Between the Flow Sensors and Valve S
- 270 EASY-nLC II: Locating a Leak Between the Flow Sensors and Valve S
- 272 Identifying a Leaking Check Valve
- 273 Troubleshooting a System Blockage
- 274 Running the Back Pressure Test for the A and B Solvent Paths
- 275 Troubleshooting a System Blockage when Test Solvent A Fails
- 281 Troubleshooting a System Blockage when Test Solvent B Fails
- 285 Troubleshooting the Autosampler Aspiration and Calibration
- 287 Checking Sample Pickup
- 288 Troubleshooting Communication Problems
- 288 Network Access
- 289 Network Connection Failures
- 290 Testing the Network Connection to the EASY-nLC Computer
- 292 Verifying that the LC/MS System Is Properly Grounded
- 293 Calibrating the Autosampler’s XYZ Robot
- 294 Replacing the Autosampler Adapter Plate
- 295 Replacing the Adapter Plate in the ASC Model Autosampler
- 296 Replacing the Adapter Plate in the ASA Model Autosampler
- 298 Managing Plate Formats
- 298 Selecting a Plate Format
- 299 Creating a New Plate Format
- 301 Deleting a Plate Format
- 302 Preparing the Autosampler for the Calibration Routines
- 304 Calibrating Plates
- 309 Calibrating the Wash Bottle and Extra Vial Positions
- 311 Remote Support
- 312 Connecting the EASY-nLC Instrument to the Support Server
- 317 Saving System Files on a USB Removable Storage Device
- 320 Downloading the Latest Firmware File
- 323 Returning the EASY-nLC Instrument
- 323 Transport Instructions
- 325 Declaration of Contamination
- 327 Error Codes
- 335 Mobile Phase Viscosity
- 337 Consumables and Replacement Parts
- 337 Ordering Information
- 338 Solvent System Schematic for the EASY-nLC II Instrument
- 340 Solvent System Schematic for the EASY-nLC 1000 Instrument
- 342 Common Replacement Parts
- 342 Common Replacement Parts for the EASY-nLC II Instrument
- 343 Common Replacement Parts for the EASY-nLC 1000 Instrument
- 345 Quick Reference Guides for Routine Maintenance
- 347 Using nanoViper Fittings Quick Reference Guide
- 347 Connecting nanoViper Fittings
- 348 nanoViper Tubing Connections
- 349 Returning the System to Atmospheric Pressure
- 349 Upstream of Valve S Solvent Lines
- 350 Downstream of Valve S Solvent Lines
- 351 Maintaining the PLU Pump Quick Reference Guide
- 353 Maintaining the PLF Pump Quick Reference Guide
- 355 Maintaining the Rotary Valves Quick Reference Guide
- 357 Replacing a Damaged Autosampler Needle
- 359 Index