Maintaining the PLF Pump Quick Reference Guide. Thermo Fisher Scientific EASY-nLC Series Troubleshooting and
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Maintaining the PLF Pump Quick Reference Guide
Replace the piston seal when you detect a leak in the pump.
Contents
1.
Removing the Pump Head and Cleaning the Piston
2.
3.
4.
5.
Preparing the Instrument for Operation
CAUTION Wear powder-free safety gloves when working with solvents and handling the wetted components of the instrument.
Replacing a piston seal requires these tools and materials.
Tools Materials
3 mm hex wrench
Piston seal tool
Pipette
Powder-free safety gloves
1/4 in. open-ended wrench LC/MS-grade methanol
#2 Phillips head screwdriver LC/MS-grade acetonitrile
Piston seal, P/N LC210 (includes the piston seal tool)
Removing the Pump Head and Cleaning the Piston
1. Set the pump piston to the fully retracted position as follows: a. On the Maintenance > Scripts page, Select Prepare in the
Category list and then select Purge Solvent in the Name list.
Press the Parameters tab. In the Purge Iterations box, enter 0.
Select the check box for the appropriate pump.
b. Press Start. The piston moves down to the 140 μL position.
2. Exit the EASY-nLC II system and turn off the power.
3. Using a #2 Phillips head screwdriver, with a quarter-turn loosen the three captive screws that secure the right side panel to the instrument. Then remove the panel.
4. Remove the tubing connected to the pump head as follows:
• If a PEEK fitting is connected to the pump head, use a
13 mm open-ended wrench, to remove it.
Figure 1. PEEK fitting on the externally threaded pump head
• If a stainless steel fitting is connected to the pump head, use a
1/4 in. open-ended wrench to remove it.
Figure 2. Stainless steel fitting on the internally threaded pump head
Stainless steel fitting connected to an internally threaded pump head
5. Using a 13 mm open-ended wrench, remove the pump head.
Figure 3. Removing the pump head
Pump head
6. Place the piston in the fully extended position as follows: a. Turn on the instrument and log in as an administrator.
b. Press Home > Overview, and then press the pump icon for the pump you want to control. The Pump dialog box opens.
c. Set the flow rate to 300 μL/min and the volume to 140 μL.
d. Press Start.
7. Clean the piston with the lint-free tissue soaked in methanol, and visually inspect the piston for any scratches.
CAUTION Take care to avoid solvent drips onto the pump PCB.
8. Return the piston to the fully retracted position as follows: a. In the Pump dialog box, set the flow rate to – 300 μL/min and the dispense volume to 140 μL. b. Press Start.
9. When the piston is fully retracted, close down the EASY-nLC system and turn off the power.
PEEK fitting connected to an externally threaded pump head
Removing the Worn Piston Seal
1. Clean the piston seal tool in a beaker filled with 100% methanol.
Figure 4. Two-component piston seal tool for the PLF model pump
2. Insert the piston seal tool into the worn piston seal and pull the piston seal out of the pump head.
Figure 5. Using the piston seal tool to remove the worn piston seal
Installing a New Piston Seal
1. Insert the guide tube into the pump head flange.
Figure 6. Guide tool inserted into the pump head flange
Guide tool
Worn piston seal
2. Using a pipette, fill the groove in the piston seal with methanol.
3. Place the new seal with the groove facing up on the piston seal tool.
Figure 7. Piston seal mounted onto the piston seal tool
4. Insert the piston seal tool into the guide tool.
Figure 8. Inserting the piston seal tool into the guide
5. Push the piston seal tool into the guide until you feel resistance.
6. Remove the guide tube and the piston seal tool, and then check that the piston seal is properly seated.
7. Using a 13 mm open-ended wrench, reconnect the pump head to the pump body. Do not connect the stainless steel tubing to the pump head.
330
Priming the Pump
1. Turn on the EASY-nLC instrument and log in as an administrator.
2. Fill the pump head with the appropriate solvent as follows: a. Press Home > Overview.
b. Press the pump icon for the pump that you want to control.
c. Set the flow rate to 300 μL/min and the volume to 140 μL.
Then press Start.
The piston moves upward to the top of the pump head.
d. Pipette the appropriate solvent into the pump head.
e. Set the flow rate to –300 μL/min and the volume to 140 μL.
Then press Start.
The piston moves down, drawing solvent into the pump head. Make sure the pump head is filled with solvent during the entire retraction of the piston.
CAUTION Be careful not to spill solvent onto the printed circuit board behind the LED panel.
3. Using a 1/4 in. open-ended wrench, reconnect the tubing to the pump head.
Preparing the Instrument for Operation
1. Reinstall the right panel.
2. Reset the usage counter for the pump as follows: a. Press Maintenance > Devices. b. Select the pump from the list of devices.
c. Press the Summary tab.
d. Press Reset.
The value in the Intermediate Volume box resets to 0.
3. To draw fresh solvent through the solvent lines and to remove air, do the following: a. Open the Parameter page for the Purge Solvent script.
b. In the Purge Iterations box, enter 10.
c. Select the check box for the appropriate pump. d. Press Start and wait for the system to perform 10 purge cycles.
4. To flush air from the pump head, do the following: a. In the Name category on the Maintenance > Scripts page, select Flush Air.
b. Press the Parameters tab.
c. In the Flush Threshold [μL] box, enter 10.
d. Press Start.
e. Wait for the script to finish.
5. Run the Leaks script for the pump as follows: a. On the Maintenance > Scripts page, select Test in the
Category list, and then select Leaks in the Name list.
b. Press the Parameters tab.
c. Select the check box for the pump with the new seal.
d. Press Start.
6. When the instrument is leak tight, it is ready for use.
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Table of contents
- 13 Contents
- 19 Preface
- 20 Related Documentation
- 20 Downloading Manuals from the Customer Manuals Web Site
- 21 Safety and Special Notices
- 23 Contacting Us
- 25 Introduction
- 26 Hardware Components
- 26 Autosampler
- 27 ASC Model—Current
- 28 ASA Model—Legacy EASY-nLC II Instruments
- 29 Solvent System Components Behind the Right Side Panel
- 29 View Behind the Right Side Panel
- 32 Syringe Pumps
- 34 Pressure Sensors
- 34 Flow Sensors
- 35 Check Valve Assemblies
- 36 Six-Port Rotary Valves
- 38 Autosampler Needle
- 39 Computer and Autosampler Behind the Left Side Panel
- 40 Back Panel
- 42 Touch-Screen Application
- 42 Description of the Touch-Screen Monitor
- 44 Logging In to the EASY-nLC Instrument for Maintenance Tasks
- 45 Closing Down the EASY-nLC Instrument
- 47 Specifications
- 47 Performance Specifications
- 48 Technical Specifications
- 51 Maintenance Schedule
- 51 Daily Maintenance
- 52 Weekly Maintenance
- 52 Quarterly Maintenance
- 53 Yearly Maintenance
- 53 Field Service Repairs
- 55 Maintenance Scripts and Service Records
- 55 Maintenance Scripts
- 56 Prepare – Purge Solvent
- 57 Prepare – Flush Air
- 58 Prepare – Precolumn Equilibration
- 60 Prepare – Analytical Column Equilibration
- 62 Prepare – Isocratic Flow
- 63 Test – MS Connection
- 66 Test – Sample Pickup
- 68 Test – Leaks
- 71 Test – Valve Check
- 72 Test – Back Pressure
- 73 Test – Autosampler Torque
- 74 Test – Pump Torque
- 74 Calibrate – Valve Tune
- 75 Calibrate – Flow Sensors
- 76 Calibrate – Reset Pressure Sensor
- 77 Calibrate – Direct Infusion
- 79 Keeping Service Records
- 80 Checking and Resetting the Device Usage Counters
- 81 Routine Maintenance
- 82 Maintaining a Clean Working Environment
- 82 Replacing the Main Power Fuse
- 84 Maintaining the Syringe Pumps
- 85 Retracting the Piston
- 86 Replacing the Piston Seal and Cleaning the Piston in a PLF Pump
- 91 Replacing the Piston Seal in a PLU Pump
- 95 Priming the Pump
- 96 Resetting the Pump Usage Counter
- 97 Removing Air After Replacing a Piston Seal or a Pump
- 97 Running the Leaks Script after Replacing a Piston Seal or a Pump
- 98 Maintaining the Rotary Valves
- 98 Cleaning the Rotor Seal and Stator
- 101 Replacing the Rotor Seal
- 102 Replacing the Stator
- 104 Replacing the Check Valves
- 105 Replacing an Inline Filter for the EASY-nLC 1000 Instrument
- 107 Using nanoViper Fittings
- 108 Replacing the Autosampler Needle
- 113 Replacing the Sample Loop
- 114 Replacing a Pressure Sensor for the PLU Pump
- 116 Replacing a Flow Sensor
- 116 Replacing a Flow Sensor in the EASY-nLC 1000 Instrument
- 120 Replacing a Flow Sensor in the EASY-nLC II Instrument
- 124 Replacing the Hard Drive
- 129 Managing the Devices List
- 131 Field Service Maintenance
- 132 Replacing the Built-In Computer
- 137 Replacing the Monitor
- 141 Replacing a Pressure Sensor for a PLF Pump
- 146 Replacing the ASC Autosampler
- 152 Upgrading from an ASA Autosampler to an ASC Autosampler
- 161 Replacing the Autosampler Cooler
- 161 Replacing the ASC Cooler
- 164 Replacing the ASA Cooler
- 171 Replacing a Rotary Valve
- 174 Replacing a Pump
- 174 Replacing a PLU Pump
- 178 Replacing a PLF Pump with a PLU Pump
- 187 Replacing a Pump PCB
- 187 Replacing a Pump PCB in the PLU Pump
- 191 Replacing a Pump PCB in the PLF Pump
- 193 Troubleshooting
- 194 Troubleshooting Tips
- 195 Autosampler Problems
- 196 Contact Closure Problems
- 196 Instrument Startup Problems
- 197 Delayed Elution
- 198 Leaks that Cause Delayed Elution
- 200 Introduced Swept Volume
- 202 Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps
- 204 System Reaches Its Maximum Pressure During the Gradient
- 207 Sample Signal Weak or Absent
- 208 Weak or Absent Signal—Sample Not Aspirated
- 209 Weak or Absent Signal—Defective Precolumn
- 210 Weak or Absent Signal—Leak Upstream of Flow Sensor B
- 211 Slow or No Pressure Increase in Subsystem A or B
- 213 Errors Reported by the Xcalibur Data System
- 218 Carryover
- 220 Spray Issues
- 221 Chromatographic Performance
- 222 Device Failures
- 226 Miscellaneous
- 228 Using the Direct Controls for Troubleshooting and Maintenance
- 228 Using the Valve Controls
- 229 Using the Pump Controls
- 229 Opening the Direct Controls for the Pumps
- 230 Using the Direct Controls To Fill or Empty Pump A or B
- 233 Using the Direct Controls To Fill or Empty Pump S
- 234 Using the Autosampler Controls
- 236 Troubleshooting a Pump that Fails the Flush Air Script
- 240 Troubleshooting a Pump that Fails the Leaks Script
- 242 Running a System Leak Test
- 245 Troubleshooting the Results of the System Leak Test
- 246 Checking Valve S for a Leak
- 246 Checking the Sample Loop Connections for a Leak
- 247 Locating a Leak in the Solvent Path from Valve B to Flow Sensor B
- 248 Locating a Leak in the Solvent Path from Pump A to Flow Sensor A
- 250 Locating a Leak In or Between Valve S and Valve W
- 258 Locating a Leak Between the Flow Sensors and Valve S
- 259 EASY-nLC 1000: Locating a Leak Between the Flow Sensors and Valve S
- 270 EASY-nLC II: Locating a Leak Between the Flow Sensors and Valve S
- 272 Identifying a Leaking Check Valve
- 273 Troubleshooting a System Blockage
- 274 Running the Back Pressure Test for the A and B Solvent Paths
- 275 Troubleshooting a System Blockage when Test Solvent A Fails
- 281 Troubleshooting a System Blockage when Test Solvent B Fails
- 285 Troubleshooting the Autosampler Aspiration and Calibration
- 287 Checking Sample Pickup
- 288 Troubleshooting Communication Problems
- 288 Network Access
- 289 Network Connection Failures
- 290 Testing the Network Connection to the EASY-nLC Computer
- 292 Verifying that the LC/MS System Is Properly Grounded
- 293 Calibrating the Autosampler’s XYZ Robot
- 294 Replacing the Autosampler Adapter Plate
- 295 Replacing the Adapter Plate in the ASC Model Autosampler
- 296 Replacing the Adapter Plate in the ASA Model Autosampler
- 298 Managing Plate Formats
- 298 Selecting a Plate Format
- 299 Creating a New Plate Format
- 301 Deleting a Plate Format
- 302 Preparing the Autosampler for the Calibration Routines
- 304 Calibrating Plates
- 309 Calibrating the Wash Bottle and Extra Vial Positions
- 311 Remote Support
- 312 Connecting the EASY-nLC Instrument to the Support Server
- 317 Saving System Files on a USB Removable Storage Device
- 320 Downloading the Latest Firmware File
- 323 Returning the EASY-nLC Instrument
- 323 Transport Instructions
- 325 Declaration of Contamination
- 327 Error Codes
- 335 Mobile Phase Viscosity
- 337 Consumables and Replacement Parts
- 337 Ordering Information
- 338 Solvent System Schematic for the EASY-nLC II Instrument
- 340 Solvent System Schematic for the EASY-nLC 1000 Instrument
- 342 Common Replacement Parts
- 342 Common Replacement Parts for the EASY-nLC II Instrument
- 343 Common Replacement Parts for the EASY-nLC 1000 Instrument
- 345 Quick Reference Guides for Routine Maintenance
- 347 Using nanoViper Fittings Quick Reference Guide
- 347 Connecting nanoViper Fittings
- 348 nanoViper Tubing Connections
- 349 Returning the System to Atmospheric Pressure
- 349 Upstream of Valve S Solvent Lines
- 350 Downstream of Valve S Solvent Lines
- 351 Maintaining the PLU Pump Quick Reference Guide
- 353 Maintaining the PLF Pump Quick Reference Guide
- 355 Maintaining the Rotary Valves Quick Reference Guide
- 357 Replacing a Damaged Autosampler Needle
- 359 Index