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System Self-Check and Checksum Test
System Self-Check
Each time the MicroFill™ is turned on, the System Self-Check is automatically performed to ensure proper instrument operation.
If the self-check passes, the Main Menu will appear and the dispenser is ready for use.
If there is a failure, the dispenser will “chirp” and display an error code. Press the
Stop
key and then look up the error code in
Appendix A
. If the dispenser fails the selfcheck, it will not perform a program operation until the error condition is cleared.
The System Self-Check can also be performed manually, by selecting
UTIL > TESTS > SLFCHK
.
Checksum Test
If the Checksum Test is performed, the initial display will show the on-board
(basecode) software part number, version number, and checksum. The second display shows the configuration software information. The Main Menu reappears at the conclusion of the test.
To run the Checksum Test, start at the Main Menu and select
UTIL > TESTS > CHKSUM
.
Dispense Precision and Accuracy Tests
Overview
Note: Disable the AutoPrime feature before conducting these tests.
Dispense
precision
is a measure of the variability of volumes dispensed from tube to tube across the manifold. The optical density of the solution in a well is proportional to the total volume of the solution in the well. If the % Coefficient of Variation (%CV) is calculated, the result is a measure of the uniformity of the distribution of dispensed volumes across the manifold. It is the ratio, expressed in percent, of the standard deviation of the distribution of fluid volumes in the wells to the mean value of volume per well. The uniformity of distribution across the manifold improves as the %CV is lowered.
Dispense
accuracy
is a measure of the average volume dispensed per well. It is independent of precision. The volume per well may vary greatly over a plate, yet the accuracy may be exact because it is an average of the volumes.
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Specifications
Precision
Note: The specifications listed here are for typical performance.
For IQ/PQ/OQ testing purposes, add 1.0% additional tolerance to the
%CV to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors.
Typical 384-well dispense precision is:
• < 2 %CV when dispensing 80 μl/well
• < 5 %CV when dispensing 20 μl/well
Typical 96-well dispense precision is:
• < 2 %CV when dispensing 160 μl/well using the 16-channel manifold
• < 5 %CV when dispensing 40 μl/well using the 16-channel manifold
• < 2 %CV when dispensing 80 μl/well using the 8-96 manifold
• < 5 %CV when dispensing 20 μl/well using the 8-96 manifold
Typical 24-well dispense precision is:
• < 2 %CV when dispensing 160 μl/well
• < 5 %CV when dispensing 40 μl/well
Accuracy
Typical 384-well dispense accuracy is:
• ± 1.25% when dispensing 80 μl/well
• ± 5% when dispensing 20 μl/well
Typical 96-well dispense accuracy is:
• ± 1.25% when dispensing 160 μl/well using the 16-channel manifold
• ± 5% when dispensing 40 μl/well using the 16-channel manifold
• ± 1.25% when dispensing 80 μl/well using the 8-96 manifold
• ± 10% when dispensing 20 μl/well using the 8-96 manifold
Typical 24-well dispense accuracy is:
• ± 1.25% when dispensing 160 μl/well using the 8-24 manifold
• ± 10% when dispensing 40 μl/well using the 8-24 manifold
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Materials
• Corning Costar #3590 96-well plates, or equivalent (16-channel and “8-96” manifold testing)
• 384-well plates (16-channel manifold testing)
• 24-well plates (“8-24” manifold testing)
• Precision balance with capacity of 100 g minimum and readability of 0.01 g resolution
• Pipettes and graduated beakers
• Microplate absorbance reader capable of dual wavelength reading at 630 and
405 (or 450) nm
If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode. To check this, select UTIL > READ and cycle through the prompts until you see READ IN RAPID MODE?”
• The test solutions described on the following page. See also the important notes below:
The absorbance of blue dye solutions should be measured at 630/450
(or 405) nm. The BioTek blue dye solution part number is 7773001.
The absorbance of yellow dye solutions should be measured at 450
(or 405)/630 nm. The BioTek yellow dye solution part number is
7120782. Note: This solution cannot be used when testing an 8-24 manifold.
The final absorbance for all dye solution concentrations should be in a range between 0.700 and 1.300 OD.
The factory determined the pass/fail specifications for the following tests using the test solutions described. You may choose to use your own solutions. If any tests fail using your own solutions, however, retry the tests using the suggested solutions.
Using pure DI water in place of these solutions is not recommended and will likely result in the failure of the unit to meet specifications.
Liquid Test Worksheet templates can be found at the end of this chapter for recording data reduction results. If your tests are failing, this information will be useful for BioTek TAC to help diagnose any problems.
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Test Solutions
For 16-Channel Manifold Testing:
20 µl Solution
• If using BioTek’s blue dye solution, mix 100 ml of DI H
2
O with 10 ml of dye solution to create a dilution of the concentrate. Mix 160 ml of DI H
2
20 ml of the diluted concentrate.
O with
• If using BioTek’s yellow dye solution, mix 940 ml of DI H
2 dye solution.
O with 60 ml of
• If using FD&C #5 yellow dye powder, mix 1 liter of DI H
2
O with 0.105 grams of dye powder.
80 µl Solution
• Mix 120 ml of DI H
2
O with 40 ml of the 20 μl solution.
For “8-96” Manifold Testing:
20 µl Solution
• If using BioTek’s blue dye solution, mix 100 ml of DI H
2
O with 10 ml of dye solution to create a dilution of the concentrate. Then mix 140 ml of DI H
2 with 80 ml of the diluted concentrate.
O
• If using BioTek’s yellow dye solution, mix 400 ml of DI H
2 dye solution.
O with 100 ml of
• If using FD&C #5 yellow dye powder, mix 1 liter of DI H
2
O with 0.350 grams of dye powder.
80 µl Solution
• Mix 120 ml of DI H
2
O with 40 ml of the 20 μl solution.
For “8-24” Manifold Testing:
40 µl Solution
• If using BioTek’s blue dye solution, mix 140 ml of DI H
2 solution.
O with 12 ml of dye
• If using FD&C #5 yellow dye powder, mix 1 liter of DI H
2 of dye powder.
O with 1.64 grams
•
Note:
BioTek’s yellow dye solution is not concentrated enough to use for this test.
160 µl Solution
• Mix 120 ml of DI H
2
O with 40 ml of the 40 μl solution.
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Dispense Precision & Accuracy Test: 16-Channel Manifold
Perform the following steps a total of four times to test two plate types (96 and 384), each with two dye solutions.
1.
Edit the Dispense program
Accuracy_Test
as follows (set
FLOW RATE
to 2 for all tests):
Test 1:
PLATE TYPE = 96, VOLUME = 40 μl/well
Test 2:
PLATE TYPE = 384, VOLUME = 20 μl/well
Test 3:
PLATE TYPE = 96, VOLUME = 160 μl/well
2.
3.
4.
5.
6.
7.
8.
Test 4:
PLATE TYPE = 384, VOLUME = 80 μl/well
Place the priming plate onto the microplate adaptor of the MicroFill™.
Prime the MicroFill™ two times with the test solution.
Tests 1
and
2:
Use
Tests 3
and
4:
Use
This will pump any residual air out of the check valves. Remove and empty the priming plate.
Place a clean, empty (96- or 384-well) microplate on the balance and then tare the balance.
Place the microplate on the MicroFill™ and run the
Accuracy_Test
program modified in step 1.
Place the plate on the balance and record the
Total Dispense Weight
.
This value will be used to calculate the % Accuracy Error in step 11.
Using a calibrated hand pipette, dispense deionized water on top of the dye solution in the wells. (Tests 1 and 2 only.)
Test 1:
Pipette 120 μl/well (resulting in 160 μl/well)
Test 2:
Pipette 60 μl/well (resulting in 80 μl/well)
Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or lightly tap the side of the plate with your finger to agitate the contents of the wells. This will result in a uniformly mixed solution and create a more uniform meniscus in each of the wells.
If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode. To check this, select UTIL > READ and cycle through the prompts.
9. Read the plate in an absorbance reader using the dual-wavelength method, to reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths on page 66. Print or export the results.
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For steps 10 and 11, enter the results in copies of the Liquid Test
Worksheets at the end of this chapter.
10.
11.
Calculate and report the Mean absorbance, Standard Deviation, and the %CV for the wells under test.
%CV
= (Standard Deviation ÷ Mean) * 100.
The
% Accuracy Error
calculation is:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 6), and divide the result by the expected weight.
Multiply the result by 100.
Example for 96-well plate, 40 μl test, Total Dispense Weight = 3.73 g:
(3.73 – 3.84) ÷ 3.84 x 100 = -2.87% = PASS (must be < ±5.0%)
The
Expected Dispense Weight
is the volume dispensed per well in ml multiplied by the number of wells dispensed. For example, if 40 μl is dispensed to 96 wells, the expected weight is 0.040 x 96 = 3.84 grams. We have calculated some expected dispense weights for you:
Test 1
grams
12.
Test 2
grams
Test 3
grams
Test 4
grams
Analyze your test results. The following is the Pass criteria for each test:
Test 1
: %CV < 6.0%* % Accuracy Error < ± 5.0%
Test 2
: %CV < 6.0%* % Accuracy Error < ± 5.0%
Test 3
: %CV < 3.0%* % Accuracy Error < ± 1.25%
Test 4
< 3.0%* % Accuracy Error < ± 1.25%
* The specifications listed on page 65 are for typical performance.
For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to the
%CV
to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors.
If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s
Technical Assistance Center.
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Dispense Precision & Accuracy Test: “8-96” Manifold
Perform the following steps two times, using two different 96-well plates:
1. Edit the Dispense program for all tests):
Accuracy_Test
as follows (set
FLOW RATE
to 2
Test 1:
VOLUME = 20 μl/well
2.
3.
4.
5.
6.
7.
8.
Test 2:
VOLUME = 80 μl/well
Place the priming plate onto the microplate adaptor of the μFill.
Prime the MicroFill™ two times with the test solution.
Test 1:
Use the 20 μl solution
Test 2:
Use the 80 μl solution
This will pump any residual air out of the check valves. Remove and empty the priming plate.
Place a clean, empty 96-well microplate on the balance and then tare the balance.
Place the microplate on the MicroFill™ and run the
Accuracy_Test
program modified in step 1.
Place the plate on the balance and record the
Total Dispense Weight.
This value will be used to calculate the % Accuracy Error in step 11.
Using a calibrated hand pipette, dispense deionized water on top of the dye solution in the wells.
Test 1:
Pipette 140 μl/well (resulting in 160 μl/well)
Test 2:
Pipette 80 μl/well (resulting in 160 μl/well)
Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or lightly tap the side of the plate with your finger to agitate the contents of the wells. This will result in a uniformly mixed solution and create a more uniform meniscus in each of the wells.
If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode. To check this, select UTIL > READ and cycle through the prompts.
9. Read the plate in an absorbance reader using the dual-wavelength method, to reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths on page 66. Print or export the results.
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71
For steps 10 and 11, enter the results in copies of the Liquid Test
Worksheets at the end of this section.
10.
11.
Calculate and report the Mean absorbance, Standard Deviation, and the %CV for the wells under test.
%CV
= (Standard Deviation ÷ Mean) * 100.
The
% Accuracy Error
calculation is:
12.
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 6), and divide the result by the expected weight.
Multiply the result by 100.
Example for 96-well plate, 20 μl test, Total Dispense Weight = 1.85 g:
(1.85 – 1.92) ÷ 1.92 x 100 = -3.64% = PASS (must be < ± 10.0%)
The
Expected Dispense Weight
is the volume dispensed per well in ml multiplied by the number of wells dispensed. For example, if 20 μl is dispensed to 96 wells, the expected weight is 0.020 x 96 = 1.92 grams. We have calculated some expected weights for you:
Test 1
grams
Test 2
grams
Analyze your test results. The following is the Pass criteria for each test:
Test 1:
%CV < 6.0%* % Accuracy Error < ± 10.0%
Test 2:
%CV < 3.0%* % Accuracy Error < ± 1.25%
* The specifications listed on page 65 are for typical performance.
For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to the
%CV
to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors.
If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s
Technical Assistance Center.
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| Chapter 5: Instrument Qualification
Dispense Precision & Accuracy Test: “8-24” Manifold
Perform the following steps two times, using two different 24-well plates:
1. Edit the Dispense program for all tests):
Accuracy_Test
as follows (set
FLOW RATE
to 2
Test 1:
VOLUME = 40 μl/well
2.
3.
4.
5.
6.
7.
8.
Test 2:
VOLUME = 160 μl/well
Place the priming plate onto the microplate adaptor of the MicroFill™.
Prime the MicroFill™ two times with the test solution.
Test 1:
Use
Test 2:
Use
This will pump any residual air out of the check valves. Remove and empty the priming plate.
Place a clean, empty 24-well microplate on the balance and then tare the balance.
Place the microplate on the MicroFill™ and run the
Accuracy_Test
program modified in step 1.
Place the plate on the balance and record the
Total Dispense Weight
.
This value will be used to calculate the % Accuracy Error in step 11.
Using a calibrated hand pipette, dispense deionized water on top of the dye solution in the wells.
Test 1:
Pipette
Test 2:
Pipette
Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or lightly tap the side of the plate with your finger to agitate the contents of the wells. This will result in a uniformly mixed solution and create a more uniform meniscus in each of the wells.
If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode. To check this, select UTIL > READ and cycle through the prompts.
9. Read the plate in an absorbance reader using the dual-wavelength method, to reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths on page 66. Print or export the results.
BioTek Instruments, Inc.
Dispense Precision and Accuracy Tests |
73
For steps 10 and 11, enter the results in the Liquid Test Worksheets at the end of this section.
10.
11.
Calculate and report the Mean absorbance, Standard Deviation, and the %CV for the wells under test.
%CV
= (Standard Deviation ÷ Mean) * 100.
The
% Accuracy Error
calculation is:
(Actual Weight – Expected Weight) ÷ Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 6), and divide the result by the expected weight.
Multiply the result by 100.
Example for 24-well plate, 160μl test, Total Dispense Weight = 3.64 g:
(3.64 – 3.84) ÷ 3.84 x 100 = -5.21% = PASS (must be < ± 10.0%)
The
Expected Dispense Weight
is the volume dispensed per well in ml multiplied by the number of wells dispensed. For example, if 40μl is dispensed to 24 wells, the expected weight is 0.040 x 24 = 0.96 grams. We have calculated some expected weights for you:
Test 1
grams
12.
Test 2
grams
Analyze your test results. The following is the Pass criteria for each test:
Test 1
: %CV < 6.0%* % Accuracy Error < ± 10.0%
Test 2
< 3.0%* % Accuracy Error < ± 1.25%
* The specifications listed on page 65 are for typical performance.
For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to the
%CV
to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors.
If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s
Technical Assistance Center.
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Documenting Test Results
The following pages contain Dispense Precision & Accuracy Test Worksheets and
Performance Verification Logs. We recommend you make copies of the appropriate pages and use them to record your calculations and test results.
• Each
Worksheet
records calculations and pass/fail test results for an individual test (e.g., “40 µl / 16-Channel Manifold / 96-Well Plate).
• Each
Performance Verification Log
records all pass/fail test results for a particular manifold type over time.
BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 40 µl / 96-Well Microplate
40 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 96)
% CV (SD
÷ Mean x 100)
% CV must be < 6.0%
%
Pass Fail
40 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 5.0%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 160 µl / 96-Well Microplate
160 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 96)
% CV (SD
÷ Mean x 100)
% CV must be < 3.0%
%
Pass Fail
160 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 1.25%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 20 µl / 384-Well Microplate
20 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 384)
% CV (SD
÷ Mean x 100)
% CV must be < 6.0%
%
Pass Fail
20 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 5.0%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
16-Channel Manifold / 80 µl / 384-Well Microplate
80 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 384)
% CV (SD
÷ Mean x 100)
% CV must be < 3.0%
%
Pass Fail
80 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 1.25%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill
Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-96” Manifold / 20 µl / 96-Well Microplate
20 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 96)
% CV (SD
÷ Mean x 100)
% CV must be < 6.0%
%
Pass Fail
20 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 10.0%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill
Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-96” Manifold / 80 µl / 96-Well Microplate
80 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 96)
% CV (SD
÷ Mean x 100)
% CV must be < 3.0%
%
Pass Fail
80 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 1.25%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill
Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-24” Manifold / 40 µl / 24-Well Microplate
40 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 24)
% CV (SD
÷ Mean x 100)
% CV must be < 6.0%
%
Pass Fail
40 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 10.0%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill
Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
MicroFill™ Dispense Precision & Accuracy Test Worksheet
“8-24” Manifold / 160 µl / 24-Well Microplate
160 µl Dispense Precision Test
Standard Deviation (SD):
Mean Absorbance (sum of all wells
÷ 24)
% CV (SD
÷ Mean x 100)
% CV must be < 3.0%
%
Pass Fail
160 µl Dispense Accuracy Test
Total Dispense (Actual) Weight:
Expected Weight:
(ml/well x number of wells dispensed)
% Accuracy Error:
(Actual Weight – Expected Weight)
÷ Expected Weight x 100
% Accuracy Error must be < 1.25%
Visual verification that no well varies considerably from the others grams grams
%
Pass Fail
Pass Fail
MicroFill
Serial Number:
Tests Performed By:
Date:
Reviewed/Approved By:
Date:
© 2008, BioTek Instruments, Inc.
Chapter 6
Troubleshooting
This chapter lists problems that you may experience with the
MicroFill™, suggests possible solutions for these problems, and provides instructions for receiving technical assistance.
Overview ............................................................................ 76
General Usage Guidelines ...................................................... 77
Before Running Any Program ............................................. 77
Preventive Maintenance .................................................... 77
Programming considerations ............................................. 77
Product Support & Service ..................................................... 78
Returning Instruments for Service/Repair ............................ 78
Contacting BioTek for Applications Support .......................... 78
Troubleshooting Charts ......................................................... 79
Dispenser Start-Up .......................................................... 79
Syringe Movement ........................................................... 79
Fluid Delivery .................................................................. 80
Fluid Leakage .................................................................. 81
Microplate Carrier Movement ............................................. 82
Dispense Manifold Movement ............................................. 82
