Table of Contents - Applied Biosystems

Table of Contents - Applied Biosystems

Q Trap LC/MS/MS TurboIonSpray Ion Source Manual TurboIonSpray Components

TurboIonSpray Components

Specifications

Ion Source Temperature Range

• Probe temperature from 0–500 °C

Liquid Chromatography

• Interfaces to any liquid chromatography system

TurboIonSpray Nebulizer (Gas 1)

• Zero grade air regulated to 100 psi

Heater Gas (Gas 2)

• Zero grade air regulated to 100 psi

Ion Source Housing

The ion source housing forms a seal with the instrument interface assembly to prevent any solvent or sample from escaping into the laboratory environment. Any liquid or vapor entering the source is safely transferred to a waste receptacle or fume hood with the aid of the source exhaust pump.

The source housing incorporates two motion mechanisms that adjust the position of the sprayer, described below, two windows to allow the operator to view the position and condition of the sprayer, a PEEK tubing transfer line, a grounded union/splitter fitting, two latches used for locking the source in place, and integrated voltage/gas connectors. The connections for the voltage, heater, and gases are made automatically once the source is locked to the interface.

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TurboIonSpray Components Q Trap LC/MS/MS TurboIonSpray Ion Source Manual

TurboIonSpray ion source

Sprayer Probe

The TurboIonSpray source contains a fixed angle adjustable sprayer probe. The sprayer probe is adjustable in two directions: toward the orifice from a scale of 0 to 12 (adjusted by the horizontal adjusting ring mounted on the TurboIonSpray probe), and up and down toward the orifice from 0 to 12 by the lateral adjustment control on the top of the ion source.

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Sprayer probe and adjustments

The TurboIonSpray nebulizer gas (GS1) and Ion Spray voltage (IS) are set at the applications computer using the software. The connections for the voltage and gas are made automatically once the source is locked to the interface.

Transfer Line and Grounded Union/Splitter

A short piece of PEEK tubing (30 cm [12"] long, 65 µm [0.0025"] ID) connects the sprayer probe inlet to a grounded fitting that can be used either as a union or a splitter.

Since the liquid being sprayed from the source is in contact with high voltages, the transfer line must be connected to the grounded fitting.

Q Trap LC/MS/MS TurboIonSpray Ion Source Manual

PEEK Tubing

Grounded Union/Splitter

TurboIonSpray Components

Peek tubing and grounded union/splitter

WARNING! The use of the PEEK tubing transfer line connected to the grounded fitting is mandatory because it prevents any exposed peripherals connected to the source from floating at high voltage.

WARNING! PEEK tubing (or fused silica) with PEEK fittings must be used as the transfer line. Metal tubing or fittings must not be used. The use of metal may result in the exposure of the operator to high voltage.

Refer to the procedure for changing the grounded fitting from a union connection to a splitter described later in this document.

Heater Probe

The heater probe temperature is set in the applications computer software by modifying the TEM parameter. There is a direct correlation between the TEM parameter value and the actual heater temperature (for example, TEM 350 is equal to 350 °C at the heater). The heater gas (Gas 2) is also set in the applications computer software. The connections for the heater and the gas are made automatically once the source is locked to the interface.

The heater probe temperature is maintained by the temperature control board (TCB) mounted behind the ion source panel (below the ion source housing). The TCB adjusts the flow of power to the heater element as a function of the difference between the actual heater temperature and the temperature setting in the applications computer. The probe temperature is monitored by a thermocouple connected directly to the heater element that maintains the temperature within ±5 degrees of the applications computer setting. The operating range for the probe is approximately 100–500 °C.

Note: The temperature is controlled by monitoring the output of a thermocouple connected to the heater surrounding the metal tube. At the temperature control board the thermocouple output is compared with the temperature setting, the difference determines the power flow to the heater.

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TurboIonSpray Components Q Trap LC/MS/MS TurboIonSpray Ion Source Manual

The operating temperature should be adjusted relative to LC mobile phase composition and flow rate. In general, a combination of heat and gas flow that allows the liquid spray to reach dryness before reaching the curtain plate will provide optimum performance.

Heater gas (Gas 2) flows of approximately 6 L/min can be considered optimum for conditions where heat is required. Ionization efficiency is improved with the input of heat for all liquid flow rates. However, at lower flow rates (< 20 µL/min), the gains are small.

Heat inputs may be desirable under low flow conditions for the additional reason of enhancing in-source (orifice-skimmer) fragmentation. Temperatures as low as 80–100 °C have proven useful for enhancing this capability (phosphopeptide mapping for instance) when operating at low flow rates.

Exhaust Pump

The TurboIonSpray source requires that the source exhaust system is properly connected

and functioning. See "Source Exhaust Pump" on page 15. A filtered nitrogen or air gas

supply (free from pump oil) is delivered to the source exhaust pump at 60 psi pressure at a flow of at least 4 L per minute. The source exhaust pump is used to vent solvent vapors that develop in the ion source plenum.

It is recommended that these vapors be passed through a trap vented to a fume hood or outside port.

WARNING! Failure to provide proper ventilation of the ion source can result in hazardous vapors being released into the laboratory environment.

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