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Biotium CF Reactive Dyes and Protein Labelling Kits

Manuals and User Guides for Biotium CF Reactive Dyes and Protein Labelling Kits. We found 1 manuals for free downloads Owner's manual

Biotium CF Reactive Dyes and Protein Labelling Kits offer versatile tools for fluorescent protein labeling. These kits utilize carbodiimide chemistry (EDC coupling) for direct conjugation or a two-stage EDC/Sulfo-NHS method for enhanced efficiency. Various CF® dyes with distinct excitation/emission wavelengths are available, enabling multicolor applications. The kits include protocols for both one-step and two-step labeling, allowing flexibility depending on your protein and experimental needs. Purification methods using Sephadex columns or ultrafiltration are described to remove excess dye.

Key Features

  • Multiple CF® dyes
  • One-step & two-step protocols
  • High labeling efficiency
  • Photostable dyes
  • Water-soluble dyes

Pages: 3 Biotium CF Reactive Dyes and Protein Labelling Kits Owner's manual

Brand: Biotium Size: 224 KB

Languages: English

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Frequently Answers and Questions

What types of proteins can be labeled with these kits?
The viewed document describes labeling proteins with activated carboxylic acids using carbodiimide chemistry. Specific proteins mentioned are antibodies, but the method adapts to other suitable proteins.
What is the role of EDC in the labeling process?
According to the viewed document, EDC (or EDAC) is a carbodiimide used for conjugating carboxylate groups to primary amines in proteins. It forms a reactive intermediate that facilitates the amide bond formation between the dye and the protein.
How is excess dye removed after labeling?
The viewed document suggests using a Sephadex® column (specific type depending on the dye used) or ultrafiltration with a molecular weight cut-off at least 3 times smaller than the protein's molecular weight.
What is the storage condition for the CF® dye amines?
The viewed document states that CF® dye amines should be stored at -20°C, protected from light. Stock solutions in DMSO or dH2O can be stored at ≤ -20°C.
Are there different protocols for labeling?
Yes, the viewed document provides protocols for both one-step direct EDC coupling and a two-step EDC/Sulfo-NHS coupling protocol.

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