20/20 n

Single Tube Luminometer

20/20n Single Tube Luminometer

Instrument Specifi cations (2030-000)

Detection Modes: Luminescence,

Fluorescence (optional)

Detector: Photomultiplier tube (PMT)

Spectral Range: 350 - 650 nm

Peak Wavelength: 420 nm

Detection Limit: 3 x 10 -18 moles ATP or

1 x 10 -21 moles Luciferase

Linear Dynamic Range: >8 logs

Read Type: Glow, Flash, Kinetic, Repeat

Sample Format: 1.5 ml microfuge tubes, 35 mm petri dishes, 12 mm x 50 mm test tubes

(optional), minicell vials (optional, Fluorescence)

Sample Adapter: Holds 1.5 ml microfuge tubes, optional 12 mm x 50 mm test tube holder available

User Interface: Touch screen navigation and operation

Data Output: Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available

External PC Requirements (optional):

Windows 98 or Windows XP

Computer Interface: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate

Power: 12 V 0.84 A Max

Auto Shutoff: Touch screen hibernates after 15 min of inactivity

Dimensions: 12.92” D x 10.44” W x 8.42” H

(32.82 cm D x 26.52 cm W x 21.39 cm H)

Weight: 8.4 lbs (3.81 kg)

Operating Temperature: 60 - 105 ºF

(15 - 40 ºC)

Warranty: One year

Approvals: CE

Single Auto Injector System (Optional)

Order Instrument P/N: 2030-001

Number of Injectors: One injector

Dispense Volume Range: Selectable between

25 - 300 μl

Dual Auto Injector System (Optional)

Order Instrument P/N: 2030-002

Number of Injectors: Two injectors

Dispense Volume Range: Selectable between

25 - 300 μl

Fluorescence Modules

Specifi cations (User Installable)

Light Source: Wavelength-matched LED

Detector: PMT

Wavelength Selection: Snap-in Fluorescence

Modules

UV Fluorescence Module (2030-040)

• Wavelengths: Ex 365 nm, Em 440 - 470 nm

• Detection Limit: 10 ng/ml dsDNA using

Hoechst Dye 33258

Blue Fluorescence Module (2030-041)

• Wavelengths: Ex 460 nm, Em 515 - 575 nm

• Detection Limit: 450 pg/ml dsDNA with

PicoGreen®

Linear Dynamic Range: 5 logs, assay dependent

Sample Adapter: 100 - 200 µl minicell vial

Read Out: Direct concentration or raw fl uorescence

Calibration: Single-point calibration

Discrete Sample Average: Sample readings averaged over 5 sec to improve accuracy

An ultra-sensitive, versatile, and affordable single tube luminometer for

Life Science research.

For the most up-to-date specifi cations, visit www.turnerbiosystems.com

Headquarters

645 N Mary Avenue | Sunnyvale CA 94085

Phone (408) 636-2400 | Toll Free (888) 636-2401

Fax (408) 737-7919 | www.turnerbiosystems.com

International Sales

For international sales, contact our headquarters or refer to our Web site at www.turnerbiosystems.com

and click the “How to Buy” tab.

For research use only. Not for use in diagnostic procedures. 20/20n is a trademark of Turner

BioSystems, Inc. All other trademarks are the sole property of their respective owners.

998-2015 Rev A - 12/2008

Product: 20/20n Single Tube Luminometer

Product Series: Luminometers

Sample Format: 1.5 ml microfuge tube, 35 mm petri dish, 12 mm x 50 mm

Technologies: test tube (optional) and 100 - 200 µl minicell vial (optional,

Fluorescence)

Luminescence and Fluorescence (optional)

Optional Features: Single or Dual Auto Injectors, Fluorescence Module

Enabling People. Enabling Science.

20/20 n

Single Tube Luminometer

20/20 n

Single Tube Luminometer

Overview

The 20/20 n Luminometer is designed to provide ultra-high performance for bioluminescent and chemiluminescent assays.

In addition to high performance, the 20/20 n

The result of this design is an instrument with superior performance that is easy to use, affordable, and can be customized to your lab’s needs.

blends user-friendly operation and a small footprint with fl exible purchasing options.

Superior Performance

The 20/20 n Luminometer provides exceptional sensitivity for all luminescent assays. Proprietary circuitry and an advanced photoncounting photomultiplier tube (PMT) produce unmatched signal-tonoise ratios. The 20/20 n has a detection limit of 1 x 10 -21 moles of luciferase, making it one of the most sensitive tube luminometers available.

With greater than 8 logs of linear dynamic range, the 20/20 n

Luminometer can measure both dim and bright samples without dilution. The extended dynamic range of the 20/20 range of virtually all chemiluminescent and bioluminescent assays, eliminating the need to dilute samples or manage detector-driven gain changes.

n spans the full

Ease of Use

The 20/20 n is designed to be put into use straight from the box without the need to read a manual or obtain special training. To achieve this plug-and-play usability, the 20/20 n combines a color touch screen with an intuitive user interface. The interface makes running samples and viewing data fast and simple while also maintaining the fl exibility needed for advanced or custom protocols.

Versatility

The 20/20 n is a versatile addition to any lab. The optional single or dual auto-injectors allow measurement of either fl ash or glow-type luminescence, while the fl exible sample compartment accommodates 1.5 ml microfuge tubes, 35 mm petri dishes, and an optional 12 mm x 50 mm test tube adapter. In addition, the bottom reading design allows for very low sample volume measurements.

For added functionality, two fl uorescent modules are available, expanding the 20/20 n functionality to common fl uorescence applications.

Fluorescence Detection

Optional Fluorescence Modules add functionality to the 20/20 n .

The modules are designed to measure the most common fl uorescence applications using excitation wavelengths in the near UV or Blue light range.

The Fluorescence Modules provide sensitive fl uorescence measurements of DNA, RNA, or protein quantitation dyes.

Fluorescence Modules

UV (Em 365 nm, Ex 440 - 470 nm)

Blue (Em 460 nm, Ex 515 - 575 nm)

Typical Fluorophores

Hoechst dye, 4-methyl-umbelliferone

(4-MU)

EGFP, rAcGFP, PicoGreen ® , RiboGreen ® ,

Fluorescein, Quant-iT™ Protein

Luminescence Light Standard

The 20/20 n Luminometer Light Standard provides an external control to verify the performance of the 20/20 n Luminometer.

Some labs require this additional verifi cation procedure.

Reading the light standard before taking measurements is a quick and easy way to ensure quality control over reproducibility, sensitivity, and linearity of the luminometer.

Enabling People. Enabling Science.

Flexible Sample

Compartment

The 20/20 n accommodates

1.5 ml microfuge tubes, 35 mm petri dishes, and 12 mm x 50 mm test tubes (with optional adapter). It is also possible to measure the light output from both liquid and solid samples.

Detection Modes

Luminescence

Luminescence Applications

• Dual Reporter Assays

• Cell Viability/ATP Assays

• Kinetics Assays

• Intercellular Ca

• Immunoassays

+2 Assays

Fluorescence (optional)

Fluorescence Applications

• DNA/RNA Quantitation

• Protein Quantitation

• Gene Expression

Data Handling

The 20/20 n stores up to 18 protocols and displays data from the last 20 measurements on the LCD screen.

Spreadsheet Interface Software or an optional thermal printer are also available for easy data exporting.

Lid Start

Adjust settings to start measurement immediately after the lid is closed.

Single or Dual Auto Injectors

(Optional)

Injectors dispense fl uids from

25 - 300 μl and enable detection of fl ash-based luminescence or tworeporter assays. Completely visible plumbing allows inspection of tubing and tips during fl ushing and priming.

Touch Screen

The touch screen allows simple navigation between screens for setting parameters, storing protocols, and starting runs.