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20/20 n
Single Tube Luminometer
20/20n Single Tube Luminometer
Instrument Specifi cations (2030-000)
Detection Modes: Luminescence,
Fluorescence (optional)
Detector: Photomultiplier tube (PMT)
Spectral Range: 350 - 650 nm
Peak Wavelength: 420 nm
Detection Limit: 3 x 10 -18 moles ATP or
1 x 10 -21 moles Luciferase
Linear Dynamic Range: >8 logs
Read Type: Glow, Flash, Kinetic, Repeat
Sample Format: 1.5 ml microfuge tubes, 35 mm petri dishes, 12 mm x 50 mm test tubes
(optional), minicell vials (optional, Fluorescence)
Sample Adapter: Holds 1.5 ml microfuge tubes, optional 12 mm x 50 mm test tube holder available
User Interface: Touch screen navigation and operation
Data Output: Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available
External PC Requirements (optional):
Windows 98 or Windows XP
Computer Interface: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate
Power: 12 V 0.84 A Max
Auto Shutoff: Touch screen hibernates after 15 min of inactivity
Dimensions: 12.92” D x 10.44” W x 8.42” H
(32.82 cm D x 26.52 cm W x 21.39 cm H)
Weight: 8.4 lbs (3.81 kg)
Operating Temperature: 60 - 105 ºF
(15 - 40 ºC)
Warranty: One year
Approvals: CE
Single Auto Injector System (Optional)
Order Instrument P/N: 2030-001
Number of Injectors: One injector
Dispense Volume Range: Selectable between
25 - 300 μl
Dual Auto Injector System (Optional)
Order Instrument P/N: 2030-002
Number of Injectors: Two injectors
Dispense Volume Range: Selectable between
25 - 300 μl
Fluorescence Modules
Specifi cations (User Installable)
Light Source: Wavelength-matched LED
Detector: PMT
Wavelength Selection: Snap-in Fluorescence
Modules
UV Fluorescence Module (2030-040)
• Wavelengths: Ex 365 nm, Em 440 - 470 nm
• Detection Limit: 10 ng/ml dsDNA using
Hoechst Dye 33258
Blue Fluorescence Module (2030-041)
• Wavelengths: Ex 460 nm, Em 515 - 575 nm
• Detection Limit: 450 pg/ml dsDNA with
PicoGreen®
Linear Dynamic Range: 5 logs, assay dependent
Sample Adapter: 100 - 200 µl minicell vial
Read Out: Direct concentration or raw fl uorescence
Calibration: Single-point calibration
Discrete Sample Average: Sample readings averaged over 5 sec to improve accuracy
An ultra-sensitive, versatile, and affordable single tube luminometer for
Life Science research.
For the most up-to-date specifi cations, visit www.turnerbiosystems.com
Headquarters
645 N Mary Avenue | Sunnyvale CA 94085
Phone (408) 636-2400 | Toll Free (888) 636-2401
Fax (408) 737-7919 | www.turnerbiosystems.com
International Sales
For international sales, contact our headquarters or refer to our Web site at www.turnerbiosystems.com
and click the “How to Buy” tab.
For research use only. Not for use in diagnostic procedures. 20/20n is a trademark of Turner
BioSystems, Inc. All other trademarks are the sole property of their respective owners.
998-2015 Rev A - 12/2008
Product: 20/20n Single Tube Luminometer
Product Series: Luminometers
Sample Format: 1.5 ml microfuge tube, 35 mm petri dish, 12 mm x 50 mm
Technologies: test tube (optional) and 100 - 200 µl minicell vial (optional,
Fluorescence)
Luminescence and Fluorescence (optional)
Optional Features: Single or Dual Auto Injectors, Fluorescence Module
Enabling People. Enabling Science.
20/20 n
Single Tube Luminometer
20/20 n
Single Tube Luminometer
Overview
The 20/20 n Luminometer is designed to provide ultra-high performance for bioluminescent and chemiluminescent assays.
In addition to high performance, the 20/20 n
The result of this design is an instrument with superior performance that is easy to use, affordable, and can be customized to your lab’s needs.
blends user-friendly operation and a small footprint with fl exible purchasing options.
Superior Performance
The 20/20 n Luminometer provides exceptional sensitivity for all luminescent assays. Proprietary circuitry and an advanced photoncounting photomultiplier tube (PMT) produce unmatched signal-tonoise ratios. The 20/20 n has a detection limit of 1 x 10 -21 moles of luciferase, making it one of the most sensitive tube luminometers available.
With greater than 8 logs of linear dynamic range, the 20/20 n
Luminometer can measure both dim and bright samples without dilution. The extended dynamic range of the 20/20 range of virtually all chemiluminescent and bioluminescent assays, eliminating the need to dilute samples or manage detector-driven gain changes.
n spans the full
Ease of Use
The 20/20 n is designed to be put into use straight from the box without the need to read a manual or obtain special training. To achieve this plug-and-play usability, the 20/20 n combines a color touch screen with an intuitive user interface. The interface makes running samples and viewing data fast and simple while also maintaining the fl exibility needed for advanced or custom protocols.
Versatility
The 20/20 n is a versatile addition to any lab. The optional single or dual auto-injectors allow measurement of either fl ash or glow-type luminescence, while the fl exible sample compartment accommodates 1.5 ml microfuge tubes, 35 mm petri dishes, and an optional 12 mm x 50 mm test tube adapter. In addition, the bottom reading design allows for very low sample volume measurements.
For added functionality, two fl uorescent modules are available, expanding the 20/20 n functionality to common fl uorescence applications.
Fluorescence Detection
Optional Fluorescence Modules add functionality to the 20/20 n .
The modules are designed to measure the most common fl uorescence applications using excitation wavelengths in the near UV or Blue light range.
The Fluorescence Modules provide sensitive fl uorescence measurements of DNA, RNA, or protein quantitation dyes.
Fluorescence Modules
UV (Em 365 nm, Ex 440 - 470 nm)
Blue (Em 460 nm, Ex 515 - 575 nm)
Typical Fluorophores
Hoechst dye, 4-methyl-umbelliferone
(4-MU)
EGFP, rAcGFP, PicoGreen ® , RiboGreen ® ,
Fluorescein, Quant-iT™ Protein
Luminescence Light Standard
The 20/20 n Luminometer Light Standard provides an external control to verify the performance of the 20/20 n Luminometer.
Some labs require this additional verifi cation procedure.
Reading the light standard before taking measurements is a quick and easy way to ensure quality control over reproducibility, sensitivity, and linearity of the luminometer.
Enabling People. Enabling Science.
Flexible Sample
Compartment
The 20/20 n accommodates
1.5 ml microfuge tubes, 35 mm petri dishes, and 12 mm x 50 mm test tubes (with optional adapter). It is also possible to measure the light output from both liquid and solid samples.
Detection Modes
Luminescence
Luminescence Applications
• Dual Reporter Assays
• Cell Viability/ATP Assays
• Kinetics Assays
• Intercellular Ca
• Immunoassays
+2 Assays
Fluorescence (optional)
Fluorescence Applications
• DNA/RNA Quantitation
• Protein Quantitation
• Gene Expression
Data Handling
The 20/20 n stores up to 18 protocols and displays data from the last 20 measurements on the LCD screen.
Spreadsheet Interface Software or an optional thermal printer are also available for easy data exporting.
Lid Start
Adjust settings to start measurement immediately after the lid is closed.
Single or Dual Auto Injectors
(Optional)
Injectors dispense fl uids from
25 - 300 μl and enable detection of fl ash-based luminescence or tworeporter assays. Completely visible plumbing allows inspection of tubing and tips during fl ushing and priming.
Touch Screen
The touch screen allows simple navigation between screens for setting parameters, storing protocols, and starting runs.
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