BX53 is the environmental conscious products according to OLYMPUS's own standards.

Main features of OLYMPUS Eco-products are as follows.

• Lead-free and arsenic-free Eco-glass for optics, such as lenses and prisms.

• Exclusion of hexavalent chrome, mercury, lead and cadmium from metal materials and surface

treatment of metal.

• Exclusion of lead solders.

• Adoption of cardboard for packing materials without styrene foam for promoting the recycling.

* A definition of exclusion depends on OLYMPUS standard. Some accessories are inapplicable.

Please visit our web site for further information: http://www.olympus.co.jp/en/eco-products/

Images are courtesy of:

Dr. Shigeo Hayashi, Dr. Kagayaki Kato, Dr. Reiko Tajiri and Mr. Hosei Wada

Laboratory for Morphogenetic Signaling

RIKEN Center for Developmental Biology (P.12, P.15 top right, P.16 top left)

Shigenobu Yonemura, Ph.D.

Electron Microscope Laboratory

RIKEN Center for Developmental Biology

(P.6 lower right, P.7, P.15 lower right, P.17 Phase contrast, P.19 top right)

Guojun Sheng, Ph.D., Yukiko Nakaya, Ph.D.

Laboratory for Early Embryogenesis

RIKEN Center for Developmental Biology (P.2, P.15 lower left, P.16 lower left)

Prof. Tadokoro, Pathology class, School of medicine, St. Marianna University (P.15)

Fumio Matsuzaki, Ph.D., Daijiro Konno, Ph.D.

Photos courtesy of:

Laboratory for Cell Asymmetry

RIKEN Center for Developmental Biology (P.6 lower left, P.15 top left, P.16 top right)

The Tokyo University (P.11 below) Junko Kyozuka, Associate Professor

Graduate School of Agricultural and Life Sciences

The Tokyo University (P.16 lower right)

Junko Kyozuka, Associate Professor, Graduate School of Agricultural and Life Sciences,

Prof. Nakatani, Biochemistry course, School of medicine, Showa University (P.13 above)

• OLYMPUS CORPORATION is ISO14001 certified.

• OLYMPUS CORPORATION is FM553994/ISO9001 certified.

• OLYMPUS CORPORATION is MD540624/ISO13485 certified.

• Illumination devices for microscope have suggested lifetimes.

Periodic inspections are required. Please visit our web site for details.

• All company and product names are registered trademarks and/or trademarks of their respective

owners.

• Images on the PC monitors are simulated.

• Specifications and appearances are subject to change without any notice or obligation on the part of

the manufacturer.

Printed in Japan M1697E-0310B

A Clear Edge in Imaging

System Microscope

BX63/BX53

BX3 Series

1

A Revolutionary New Standard in Accuracy and Imaging Efficiency

User-friendly design assures outstanding operating ease and flexibility for brightfield/darkfield and fluorescence imaging in a wide range of research settings. With a choice of models and configuration options, there's an

Olympus research microscope to meet your individual needs.

2

3

Accuracy & Ease of Use

Advanced Sensitivity in Fluorescence Imaging

Accuracy

DM595

BA600to assure a high S/N ratio.

U-FUW

100

90

80

70

60

50

40

30

20

10

0

300 350 400 450 500

Wavelength (nm)

550 600

U-FMCHE

100

90

80

70

60

50

40

30

20

10

0

500 550 600 650 700

Wavelength (nm)

750 800

DM410

BA420IF

BP340-

390

Fluorescent Mirror Units with the Latest Coating Technology

Olympus applies an outstanding filter coating technology to all fluorescence mirror units in order to produce high transmissions, sharp cut-offs and efficient detections of fluorescence.

Fluorescence light for observation

Light source

Emission filter

Dichromatic mirror

Excitation filter

Stray light

Low reflection function

Excitation light:

Illumination light

Objective

Specimen

Stray Light Reduction Equipped on

All Mirror Units

The low reflection function eliminates over

99% of stray light, producing a high S/N ratio.

Surface of Fly’s-eye Lens,

Enlarged Image

New Fluorescence Illuminators with Fly’s-eye Lens

Fluorescence illuminators BX3-RFAA and

BX3-RFAS equipped with a Fly’s-eye lens provide uniform illumination. Even, uniform fluorescence illumination facilitates postimaging enhancements and processing.

High Transmission Objectives with

Reduced Autofluorescence

Olympus UIS2 objectives are made with superior low-autofluorescence glass, antireflection coats, and lens joining materials, improving the S/N ratio. Effective detections of subtle fluorescence emissions even with weak excitation light deliver ideal performance in fluorescence imaging.

Condenser Design to Reduce

Back-reflections

The motorized universal condenser is designed to reduce back-reflections and autofluorescence by swinging its top lens out, automatically closing its diaphragm to the minimum, and locating the wheel in between two positions.

Motorized Sensors with Power-off

Functions

The sensor for the motorized fluorescence illuminator BX3-RFAA turns the light on only upon mirror unit changeover commands, and turns it off as soon as the command is completed. This function assures that stray light from the sensor is terminated during observation.

Low Autofluorescence Immersion Oil

This immersion oil is specifically designed to reduce the autofluorescence improved S/N ratio. Insusceptible to crystallization, it is an ideal oil for long time observations.

normally associated with such oils, for

4

5

Reliability and Precision in an Easy-to-use Package

Upgraded Accuracy

The BX63 microscope features a fixed stage and focusing nosepiece for maximum stability and accurate imaging.

An ultrasonic drive system enables fine, smooth control of specimen position.

Accuracy & Ease of Use

Effortless Imaging

The cellSens software allows researchers to perform effortless imaging.

Accuracy Ease of Use

Brain section of mouse at embryonic day 15 (Cy3) NRK-52E cells (Alexa Fluor 488/Alexa Fluor 546)

Further Fluency in Fluorescence

Integration

The Olympus 8-position fluorescence illuminator allows flexible responses to various fluorescence specimens, with easily replaceable mirror units.

Observations are accelerated further with less need for changing mirror units for specimens such as multicolors and FISH.

6

BX63

Intelligent Design for Maximum

Configuration Flexibility and Operating Ease

The BX63 offers outstanding stability and imaging precision for research applications, with the convenience of a touch screen interface for easy operation. Additionally, it features a detachable controller that can be positioned to suit the operator's preference or workflow requirements.

Accuracy & Ease of Use BX63

Stability for Improved Imaging Reliability

The Olympus BX63 is the choice for today’s motorized microscope.

By incorporating a motorized nosepiece and securely fastening the stage on three points, the microscope is even more stabilized.

The smooth, high-precision drive of the fixed ultrasonic stage reduces vibrations, further advancing the quality of image acquisition.

Displacement

Large

Small

BX61 BX63

Stability Simulation in Comparison to BX61

Intelligent Microscope BX63 +

Digital Camera DP72

7

Intuitive Touch Panel Controller for Enhanced Workspace and

Workflow Flexibility

Programmable controls greatly simplify repetitive observation and imaging tasks.

In Navigation mode, the interface is context-sensitive, and displays only the functions that are relevant to the currently selected observation method.

In Full Operation mode, it allows researchers access to the full range of functions and customization options available.

The Detachable Controller Customizes Workspace into an Efficient Microscope Workstation

The controller U-MCZ can be removed and attached anywhere on the microscope, enabling the researcher to create a working environment all his/her own.

The XY-controller of the ultrasonic stage can also be attached to the user’s preferred side, allowing the simultaneous operation of the controller and the mouse.

The controller’s functionally-placed switches simplify observation method/objective/ mirror unit changeovers, light intensity adjustments and live/archive image selections.

Touch Panel Controller

U-MCZ

+BX3-SSU Ultrasonic Stage XY-controller

Configuration

8

Motorized Units Facilitate Operations

With the BX63, a diverse lineup of motorized units are available.

The researcher has a choice of three controls—the touch panel controller, the controller/U-MCZ, or the imaging software cellSens—for his/her observation method settings, microscopic operations and digital imaging. Observations are made so much faster and simpler, thanks to the interlocked motorized units working together to satisfy even the most demanding applications.

Accuracy & Ease of Use BX63

Motorized Fluorescence Illuminator/

BX3-RFAA

The flexibility of the motorized fluorescence illuminator accommodates multi-color stained specimens.

The 8-position mirror units permit quick changeover of fluorescence colors.

Motorized Attenuator Wheel/U-AW

The Olympus BX63 has a motorized ND filter wheel for fluorescence and transmitted light intensity adjustments.

* Special adapters are required for mounting (U-LHEAD for fluorescence, and U-LH100ADP for transmitted light).

Motorized Seven Position Nosepiece/

U-D7REA

Equipped with a DIC slider slot, this revolving nosepiece allows simultaneous attachment of seven objectives. It is especially suitable for continuous observations from low to high magnifications and combining specific objectives, such as polarized light observations.

9

Touch Panel Controller

Controller/U-MCZ

Software cellSens

Microscope Frame Equipped with

Motorized Focus and Field Diaphragm

This unit incorporates a high-speed, highprecision motorized focusing nosepiece with

0.01 µm resolution and 20 mm vertical strokes. The field diaphragm adjustment of transmitted light is also motorized.

Motorized Universal Condenser/

BX3-UCD8A

By integrating with designated optical components, the motorized universal condenser accommodates various kinds of transmitted light observation, from brightfield to differential interference contrast and phase contrast.

Scanning Stage with Ultrasonic/

BX3-SSU

The ultrasonic stage delivers high-precision

XY control. The XY-controller can be mounted on the controller/U-MCZ for the

BX63 and worked like conventional stage handles.

10

BX53

A Great Solution for System Flexibility with Comfortable Operability

The BX53 is a versatile system microscope that can be configured to meet virtually any research need. It supports a wide range of fluorescence imaging applications, and has a range of advanced features for enhanced operating ease and process flexibility.

System Microscope BX53 +

Digital Camera DP72

11

Accuracy & Ease of Use BX53

Customizable Control Layout

Light intensity now can be controlled with the dial in front, and transmitted filters and fluorescence shutters are operable from either side. Detachable fine focus handle can be attached on either side of the microscope based on operator preference. The BX53 frees the operator to create his/her own working environment with the microscope.

Further Ease in Imaging with

Multi-stained Specimens

The 8-position fluorescence illuminator allows flexible responses to various fluorescence specimens.

Mirror units can easily be replaced.

Automatic Switching of DIC Prisms

Switching objectives on the motorized 7-position revolving nosepiece, integrated with the motorized universal condenser, enables an automatic switch to the optimal DIC prism. Simplified prism switches accelerate observations.

Saves Microscope Information with Coded Units

The imaging software cellSens integrated with the coded fluorescence illuminator BX3-RFAS and the motorized 7-position nosepiece U-D7RES can automatically store fluorescence mirror unit and objective data with the images, facilitating post-imaging treatments.

Objective Data Mirror Unit Data Captured Image

Imaging Software

Changing Objectives with Coded

Nosepiece, Automatic Switchovers of

DIC Elements

Energy Saving Switch Turns off

Automatically

The motion sensor detects when an operator leaves and will automatically turn off the transmitted light lamp after around 30 minutes. The energysaving switch conserves energy and lamp lifetime.

Optimum Contrast Observation

System Microscope BX53

12

UIS2 Objectives Deliver Optimal Performance in Wider Wavelength Spectrum

■ UPLSAPO Series

Thanks to the application of the original Olympus UW multi-coatings, these Super

Apochromat objectives compensate for both spherical and chromatic aberrations from the UV to the near infrared region. Their sensitivity to fluorescence emissions ensures the acquisition of sharp, clear images, without color shift, even in brightfield and Nomarski DIC observations. For quality and performance, they offer solutions for digital imaging needs.

■

PLAPON Series

Designed for unsurpassed resolution and contrast, these Plan Apochromat objectives keep chromatic aberration down to a minimum.

The PLAPON60XOSC objective has two improvements, chromatic aberration compensation at 405 nm–650 nm and image-forming performance at 405 nm.

13

■ UPLFLN (UPLFLN-PH)

Series

These plan objectives also provide flat images with high transmission up to the near infrared region of the spectrum. With their high

S/N ratio, excellent resolution and high contrast imaging, they are especially effective in brightfield and Nomarski DIC observations. The UPLFLN-PH series is optimized for phase contrast observation.

■

PLN(PLN-PH) Series

Ideal for a range of biological applications, these high quality objectives feature excellent flatness up to F.N.

22 in transmitted brightfield

(phase contrast) observation.

The PLN-PH series is specifically designed for phase contrast work.

UIS2 objectives

Objective

UPLSAPO 4X 0.16

UPLSAPO 10X2 0.4

13

3.1

26.5

—

26.5

0.17

UPLSAPO 20X 0.75

0.6

26.5

0.17

UPLSAPO 20XO 0.85

0.17

26.5

— Oil

UPLSAPO 40X2 0.95

0.18

26.5

0.11-0.23

UPLSAPO 60XW 1.20

0.28

26.5

0.15-0.21

Water

UPLSAPO 60XO

UPLSAPO 100XO

N.A.

1.35

1.40

PLAPON 1.25X

0.04

PLAPON 2X 0.08

W.D.

(mm)

0.15

0.13

5

6.2

F.N.

26.5

26.5

26.5

26.5

Cover glass Immerthickness sion

(mm)

Spring

0.17

0.17

—

—

Oil

Oil

_

_

_

_

_

_

PLAPON 60XO 1.42

0.15

26.5

0.17

PLAPON 60XOSC 1.4

0.12

22 0.17

UPLFLN 4X 0.13

17 26.5

—

Oil

Oil

_

_

UPLFLN 10X2 0.3

UPLFLN 20X 0.5

10

2.1

26.5

—

26.5

0.17

UPLFLN 40X 0.75

0.51

26.5

0.17

UPLFLN 40XO 1.3

0.2

26.5

0.17

UPLFLN 60X 0.9

0.2

26.5

0.11-0.23

UPLFLN 60XOI 1.25-0.65

0.12

26.5

0.17

UPLFLN 100XO2 1.3

0.2

UPLFLN 100XOI2 1.3-0.6

0.2

26.5

26.5

0.17

0.17

UPLFLN 10X2PH 0.3

UPLFLN 20XPH 0.5

10

2.1

26.5

—

26.5

0.17

UPLFLN 40XPH 0.75

0.51

26.5

0.17

UPLFLN 60XOIPH 1.25-0.65

0.12

26.5

0.17

UPLFLN 100XO2PH 1.3

UPLFLN 4XP 0.13

UPLFLN 10XP 0.3

UPLFLN 20XP 0.5

0.2

17

10

2.1

26.5

26.5

26.5

0.17

—

—

26.5

0.17

UPLFLN 40XP 0.75

0.51

26.5

0.17

UPLFLN 100XOP 1.3

0.2

26.5

0.17

Oil

Oil

Oil

Oil

Oil

Oil

Oil

_

_

_

_

_

_

_

_

_

_

_

_

_

_

Correction ring

Iris diaphragm

Water proof and oil proof function

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

_

Objective N.A.

W.D.

(mm)

F.N.

PLN 2X

PLN 4X

PLN 10X

PLN 20X

0.06

0.1

0.25

0.4

PLN 40X 0.65

0.6

PLN 50XOI 0.9-0.5

0.2

PLN 100XO

PLN 10XPH

1.25

0.25

0.15

10.6

5.8

18.5

10.6

1.2

PLN 20XPH

PLN 40XPH

0.4

0.65

PLN 100XOPH 1.25

PLN 4XP 0.1

ACHN 10XP 0.25

1.2

0.6

0.15

18.5

6

ACHN 20XP

ACHN 40XP

0.4

0.65

ACHN 100XOP 1.25

3

0.45

0.13

22

22

22

22

22

22

22

22

22

22

22

22

22

22

22

22

MPLAPON100X 0.95 0.3

MPLAPON100XO 1.40 0.1

26.5

26.5

MPLFLN2.5X

0.08 10.7

26.5

0.17

0.17

—

—

—

0.17

—

—

—

Cover glass Immerthickness sion

(mm)

Spring

—

—

—

0.17

_

_

Oil

Oil

Oil

_

_

_

_

_

0.17

0.17

— Oil

_

_

0

0

—

Oil

MPLFLN10X 0.30 11

MPLFLN20X 0.45 3.1

26.5

26.5

—

0

MPLFLN40X 0.75 0.63 26.5 0

MPLFLN100X 0.90 1

MPLN5X 0.10 20

26.5

22

0

—

UAPON20XW340 0.70 0.35

UAPON40XO340 1.35 0.1

22

22

0.17

0.17

Water

Oil

UAPON40XW340 1.15 0.25

22 0.13-0.25

Water

All UIS2 objectives and WHN eyepieces: lead-free eco-glass

UIS objective

Objective

PLFL 100X

N.A.

0.95

W.D.

(mm)

0.2

_

_

_

F.N.

Cover glass Immerthickness sion

(mm)

26.5

0.14-0.2

Spring

_

Correction ring

Iris diaphragm

Water proof and oil proof function

_

Correction ring

_

_

Iris diaphragm

_

_

_

_

_

_

_

_

Oil proof cap

Observation Tubes / Eyepoint Adjusters

A wide range of observation tubes is available for the BX3 series, including wide field binocular and trinocular types, various tilting tubes, and tubes for observation of upright images in which the specimen and the observed image move in the same direction.

q w u y

!0

qU-TTBI/U-ETBI wU-TTLBI eU-TTR-2 rU-SWETR tU-SWETTR-5 yU-BI30-2 uU-TBI-3 iU-TR30-2/U-TR30NIR oU-SWTR-3 !0U-TBI-3-CLI !1U-ETR-4 !2U-EPA2 !3U-EPAL-2

Eyepieces

Eyepieces maintain image flatness even when a reflected light illuminator or other intermediate tube is attached. The two available types are F.N. 22 and F.N. 26.5.

Eyepiece specifications

Item

Widefield

Name

WHN10X

WHN10X-H

CROSSWHN10X

Super widefield SWH10x-H

MICROSWH10X

CROSSSWH10X

F.N.

22

22

22

26.5

26.5

26.5

Diopter

-8 — +5

-8 — +5

-8 — +2

-8 — +2

-8 — +2

Micrometer (ømm)

24

24

— i

!1

e o r

!2

!3

t

14

World-renowned Optical Performance Accommodates

Various Observation Styles

Fluorescence

Olympus Takes Fluorescence Observation to Another Plane

Nomarski DIC

Image Optimization According to Specimen Characteristics

Brain section of mouse at embryonic day 15 (GFP) Tracheal primordia of Drosophila embryo (GFP) Distal Tip of a Drosophila limb (GFP) Brain section of mouse at embryonic day 15

Gastrulation stage chicken embryo (DAPI/Alexa Fluor 488/Alexa Fluor 594) NRK-52E cells (Alexa Fluor 488/Alexa Fluor 546)

A total of three types of reflected illuminators are available, the motorized fluorescence illuminator BX3-RFAA, the coded fluorescence illuminator BX3-RFAS, and the universal reflected illuminator BX3-URA.

Eight fluorescence mirror units can be attached for comfortable multi-color fluorescence observations. Fly’s-eye lenses, that are equipped with both BX3-RFAA and BX3-RFAS, and highperformance filters that come with mirror units realize bright, efficient fluorescence observations. Storing fluorescence mirror unit data

(used in imaging) together with the images is possible by integrating coded fluorescence illuminator and motorized fluorescence

Illuminator.

BX3-URA

BX3-RFAS

BX3-RFAA

15

Blood island at stage 12 of chicken development

BX3-UCD8A

A shoot apical meristem of rice

U-CO1.25x

U-TLD U-TLO

U-UCD8-2

Olympus has prepared a wider selection of DIC sliders with varied shearing value for acquiring optimal specimen images. The U-DICT and the U-DICTS are designed for all-round performance. The

U-DICTHC optimizes high-contrast observations of thin specimens, and the U-DICTHR, high resolution with less glare for thick specimens.

Two types of condensers are also available: the 8-position universal condenser U-UCD8-2 and the motorized universal condenser

BX3-UCD8A, both for various observations (brightfield, darkfield, phase contrast, DIC and simple polarized light).

q w e r qU-DICT wU-DICTS eU-DICTHR rU-DICTHC

16

Brightfield

Brighter Images, with Superb Resolution/flatness at All Magnifications

A diverse condenser lineup includes the achromatic aplanatic

U-AAC, delivering excellent resolution and flatness from low to high magnifications, the swing-out U-SC3 accommodating 1.25x to 100x, the low magnification U-LC for w r continuous 2x to 100x (Dry) observations, and the ultra low magnification U-ULC-2.

q e t qU-SC3 wU-ULC-2 eU-AC2 rU-AAC tU-LC Cerebrum (Bodian Stain)

Liver (Azan Stain) Pancreas (HE Stain)

Phase Contrast

Ideal Phase Contrast Observation with Excellent

Image Clarity

High contrast phase imaging allows close observation of the cell interior and of live bacteria. Using UPLFLN-PH or PLN-PH series objectives, phase contrast observation from 10x up to 100x is achievable. With the U-PCD2 phase/darkfield condenser, users can view specimens in brightfield or darkfield. Simultaneous observation with reflected light fluorescence microscopy is also possible.

U-PCD2

NRK-52E cells

NRK-52E cells

17

NRK-52E cells

Polarized Light

Polarizing Observation for Wide-area Retardation

Measurement

Tooth, bone, muscle tissue, nerve tissue, actomyosin fiber and mitotic spindle can all be observed, without staining. There are intermediate attachments (U-OPA/U-CPA) for orthoscopic and orthoscopic/conoscopic viewing.

Various compensators make it w t possible to observe a wide range of retardation. Also available is a q condenser exclusively for polarization observation, revolving nosepiece, r rotating stage, objectives and simple polarizing attachment.

e qU-POC-2 wU-CPA eU-OPA rU-AN360P-2 tU-P4RE

Amyloid

Asbestos Urate crystals

Darkfield

High-quality Darkfield Effect at All Magnifications

Two darkfield condensers are provided: the dry darkfield condenser

(U-DCD), for magnifications from 10x to 100x (up to N.A. 0.80); and the oil immersion darkfield condenser (U-DCW), for magnifications from 20x to 100x (up to N.A. 1.2).

* Please consult your nearest Olympus representative for applicable objectives.

U-DCD U-DCW

Spirogyra

Water flea

18

Microscope Digital Cameras to Fulfill Diverse Needs

DP72

High-speed, High-definition Image Capture Provides Smooth Fluorescence Imaging

Thanks to its high-speed hardware, the DP72 can capture highresolution images equivalent to 12.8 million pixels* in around 2.5

seconds. The camera's high sensitivity and low noise (equivalent to the level of ISO 1600) ensure clear fluorescence imaging, while the resolution quality allows precise representation of particular specimen areas.

*By shifting the pixels of the 1.45 million pixel 2/3 inch CCD (one pixel = 6.45 um), it is possible to record still images equivalent to the maximum image recording size (4140 x 3096) or effective image size of 12.8 million pixels.

DP25

High-definition, High-resolution 5-million Pixel Technology

The DP25 provides live display at a high frame rate of 8 fps with exceptional quality of 2560 x 1920 pixels. In addition, the DP25 is equipped with a color profile that provides full-color images in realtime, allowing true color reproduction of specimens. It can easily be connected with just one cable (6-pin) to a PC with a FireWire

(IEEE1394) port. It can also connect to a laptop PC via a FireWire

(IEEE1394a) PC card.

DP21

The Optimal Stand-alone Model for Conferences

The DP21 is a stand-alone digital camera with a convenient handset for simple operations from observations to imaging. Its accurate color reproductions and smooth, high-definition live image displays are ideal for small discussion groups and conferences. Optional cellSens imaging software platform also allows operation via computers.

Camera Adapters

The single port tube of the trinocular tube is detachable, and can be used with various cameras through a range of adapters.

19

Camera Adapter

(projection lens)

U-TV1xC

U-TV1x-2+U-CMAD3

U-TV0.63xC

U-TV0.5xC-3

U-TV0.35xC

Projection

Magnifications

1x

1x

0.63x

0.5x

0.35x

Projection Area (F.N.)

2/3" CCD 1/2" CCD 1/3" CCD

11 8 6

11

17.5

8

12.7

6

9.5

22

22

16

16

12

12

Practical Field of View (mm) =

Projection Area (field number)

Objective Magnifications

U-TV0.63

×C

U-TV1 ×C

U-TV1 ×-2+

U-CMAD3

U-TV0.35

×C-2 U-TV0.5×C-3

Imaging Software / cellSens

cellSens is an imaging software available in three versions to meet individual workflow needs.

"Entry" is used for simple image acquisition. “Standard” provides simple operation for imaging documentation.

“Dimension” allows for the control of the complete workflow from image capture to analysis. With GUI customization, working environments for both Standard and Dimension can be optimized according to workflow. cellSens frees the researcher to concentrate on his/her creative work.

*cellSens versions 1.4 and later support the BX3 series. cellSens functions

Layout User experience customization

View Overlay multiple images

Tile view (multiple images in a single data set shown side by side)

Image acquisition

Image processing

Image analysis

Documentation

Remote viewing

Slice view for orthogonal plane viewing of 3D or timelapse data sets

Snap/Movie acquisition

Multi-dimensional (xyzt and wavelength)

Automated multiple image alignment (requires motorized stage)

Instantly create EFI image (manual or motorized Z)

Geometry/combine/filter processing

Fluorescence unmixing

3D deconvolution (constrained iterative deconvolution)

Region and line measurements

Object analysis and classification

Colocalization

Automatically compose Word reports

Database image and data management solution for microscopy

NetCam (live image data transmission over the internet) d

Dimension Standar

✓

✓

✓

✓

✓

Multichannel 5D

✓

✓

✓

✓

Multiposition

✓

✓

Multichannel 5D

CI Deconvolution

✓

Count & Measure

Multichannel 5D

✓

Database

✓

✓

✓

Entry

✓

✓

✓

20

BX63 system diagram

TR-Adapter

U-FMT

F mount adapter

U-TMAD

T mount adapter

U-SMAD

Sony mount adapter

U-BMAD

Bayonet mount adapter

U-CMAD3

C mount adapter

◆ WHN10X-H,

WHN10X

Eyepieces

U-CT30-2

Centering telescope

* WHN10X,

WHN10X-H,

Eyepieces

U-CT30-2 telescope

U-TVZ

Zoom camera port

U-TV1x-2

Direct image camera port U-DPCAD

Dual port tube with C mounts

U-CMDPTS

C mount adapter for U-DPTS

U-DPTS

Multi double port tube

U-CMDPTS

C mount adapter for U-DPTS

U-TV0.25xC

C mount camera port with 0.25x lens

◆

U-ETR-4* 1

Erect image trinocular tube

U-TR30NIR

Trinocular tube

U-TR30-2

Trinocular tube

U-TTR-2

Tilting trinocular tube

U-BI30-2

Binocular tube

U-TBI-3* 1

Tilting binocular tube

B

U-IFCBL200

Interface cable,

200 cm

U-POT

Polarizer

U-MCZ

Controller

Filter (ø45)

U-FC

Filter cassette

BX63F

BX63 frame

Touch panel controller

(attached to BX63F)

U-LH100ADP

LH100 adapter

A

U-D7RES

Coded 7-position nosepiece

E

U-GAN

Gout analyzer

U-ANT

Analyzer for transmitted light

U-DICT

DIC slider for transmitted light

U-DICTS

Shift DIC slider for transmitted light

U-DICTHR

High resolution DIC slider for transmitted light

U-DICTHC

High contrast DIC slider for transmitted light

U-D7REA

Motorized 7-position nosepiece

BX3-ARM

Standard arm

E

OBJECTIVES

A

U-IFCBL50

Interface cable, 50 cm

A

U-AW

Motorized attenuator wheel

U-LHLEDC

LED lamp housing

U-IFCBL15

Interface cable, 15 cm

U-IFCBL200

Interface cable, 200 cm

A

B

U-LH100-3

100 W halogen lamp housing

U-RMT

Extension cord

C

CAMERAS

U-TV0.35xC-2

C mount camera port with 0.35x lens

U-TBI-3-CLI * 1

Tilting binocular tube

BX3-RFAS

Coded fluorescence illuminator

A

E

Mirror units

U-TV0.5xC-3

C mount camera port with 0.5x lens

U-TTBI

Ergonomic binocular tube

U-AN-2

Analyzer slider

U-TV0.63xC

C mount camera port with 0.63x lens

U-LHEAD

Extension adapter for lamp housing

E

BX3-RFAA

Motorized fluorescence illuminator

A

U-IFCBL15

Interface cable, 15 cm

U-ETBI

Ergonomic erect image binocular tube

U-TV1XC

C mount camera adapter

◆

U-TTLBI* 2

Tilting, telescopic, lifting binocular tube

U-DULHA

Double lamp housing adapter

U-AW

Motorized attenuator wheel

U-IFCBL15

Interface cable, 15 cm

U-IFCBL200

Interface cable, 200 cm

A

B

B

C

D

C1394A

Interface cable

BX3-CBH

Control box

U-KPA

Intermediate attachment for simple polarizing observation

U-CA

Magnification changer

U-ANT

Analyzer for transmitted light

U-EPA2

Eyepoint adjuster

U-EPAL-2

Eyepoint adjuster

U-LH75XEAPO

75 W xenon apo lamp housing

U-LH100HGAPO

100 W mercury apo lamp housing

U-RX-T

Power supply unit for xenon lamp

U-RFL-T

LIFE TIME

BURNER ON

U-LH100HG

100 W mercury lamp housing

U-RFL-T

Power supply unit for mercury lamp

PC (software)

U-RFL-T

LIFE TIME

BURNER ON

U-HLD-4,

U-HLDT-4

Specimen holder

U-HRD-4,

U-HRDT-4

Specimen holder

U-HLS-4,

U-HLST-4

Specimen holder

IX2-GCP

Glass stage insert plate

IX-CP50

Insert plate

IX-SCL

Slide clip

U-TLO

Oil top lens

U-TLD

Dry top lens

U-CST

Centering target

D

XY-controller

(attached to BX3-SSU)

U-SC3

Swing-out condenser

U-AAC

Achromatic/

Aplanatic condenser

U-ULC-2

Ultra low condenser

U-DCD

Darkfield condenser, dry

U-DCW

Darkfield condenser, oil

U-UCDTP530

Tint plate for sensitive color observation

U-UCD8-2

8-position universal condenser

U-SHG

U-SHGT

Rubber grips

U-SVLO

Oil rectangular stage with left-hand control

U-SVRO

Oil rectangular stage with right-hand control

U-SVLB-4

Mechanical stage with left-hand control

U-SVRB-4

Mechanical stage with right-hand control

Control box

(attached to BX3-SSU)

BX3-SSU

Scanning stage with ultrasonic

IX-SVL2* 3

Cross stage with short left handle

BX3-STAD

Stage adapter

* 1 Slight vignetting may occur in combination with an additional intermediate attachment or fluorescence illuminator. * 2 Require an additional intermediate attachment or fluorescence illuminator. * 3 Cannot be used with BX3-UCD8A.

Optical elements

U-CO1.25x

Low magnification conversion lens for UCD

BX3-UCD8A

Motorized universal condenser

A

U-IFCBL15

Interface cable, 15 cm

21 22

BX53 system diagram

TR-Adapter

◆

WHN10X-H,

WHN10X

Eyepieces

U-CT30-2

Centering telescope

* WHN10X, WHN10X-H,

Eyepieces

U-CT30-2

Centering telescope

▲ SWH10X-H,

Eyepieces

U-CT30-2 telescope

U-SWETTR-5

Super widefield erect image tilting trinocular observation tube

U-FMT

F mount adapter

U-TMAD

T mount adapter

U-TVZ

Zoom camera port U-TV1x-2

Direct image camera port

U-SWETR

Super widefield erect image trinocular observation tube

U-SWTR-3

Super widefield trinocular tube

U-SMAD

Sony mount adapter

U-BMAD

Bayonet mount adapter

U-DPCAD

Dual port tube with C mounts

U-CMAD3

C mount adapter

U-ETR-4* 1

Erect image trinocular tube

*

U-TR30NIR

Trinocular tube

*

*

U-TR30-2

Trinocular tube

U-TTR-2

Tilting trinocular tube

◆

U-CMDPTS

C mount adapter for U-DPTS

U-DPTS

Multi double port tube

U-CMDPTS

C mount adapter for U-DPTS

U-BI30-2

Binocular tube

*

U-TBI-3* 1

Tilting binocular tube

*

U-TV0.25xC

C mount camera port with

0.25x lens

U-TV0.35xC-2

C mount camera port with 0.35x lens

U-TBI-3-CLI* 1

Tilting binocular tube

*

*

U-TTBI

Ergonomic binocular tube

CAMERAS

U-TV0.5xC-3

C mount camera port with 0.5x lens

U-TV0.63xC-3

C mount camera port with 0.63x lens

*

U-ETBI

Ergonomic erect image binocular tube

◆

U-TV1xC

C mount camera adapter

U-TTLBI* 2

Tilting, telescopic, lifting binocular tube

U-GAN

Gout analyzer

U-ANT

Analyzer for transmitted light

U-DICT

DIC slider for transmitted light

U-DICTS

Shift DIC slider for transmitted light

U-DICTHR

High resolution DIC slider for transmitted light

U-DICTHC

High contrast DIC slider for transmitted light

U-ANT

Analyzer for transmitted light

U-TAD

Plate adapter

COMPENSATORS

U-SRG2

Rotatable graduated stage

U-FMP

Mechanical stage

OBJECTIVES

U-SRP

Precision rotatable stage

U-SP

Plain stage

A

U-D7REA

Motorized

7-position nosepiece

C

U-CST

Centering target

U-SHG

Rubber grip

U-SHGT

Rubber grip

B

U-D7RES

Coded 7-position nosepiece

BX3-ARM

Standard arm

BX53F

BX53 frame

U-POT

Polarizer

U-FC

Filter cassette

U-LH100-3

100 W halogen lamp housing

Filter (ø45)

U-SVRO

Oil rectangular stage with right-hand control

U-HLD-4,

U-HLDT-4

Specimen holder

U-SVLO

Oil rectangular stage with left-hand control

U-D7RE

Septuple revolving nosepiece for

DIC/simple POL

C

Mirror units

A

C B

C

U-HRD-4,

U-HRDT-4

Specimen holder

U-SVRB-4

Mechanical stages with right-hand control

U-D6RE

Sextuple revolving nosepiece for

DIC/simple POL

BX3-RFAA* 4

Motorized fluorescence illuminator

U-AN-2

Analyzer slider

BX3-RFAS* 4

Coded fluorescence illuminator

U-AN-2

Analyzer slider

BX3-URA

Universal reflected illuminator

U-AN-2

Analyzer slider

BX3-25ND6

ND filter

BX3-25ND25

ND filter

U-SVLB-4

Mechanical stages with left-hand control

U-P6RE

Centerable sextuple revolving nosepiece

U-HLS-4,

U-HLST-4

Specimen holder

* 1 Slight vignetting may occur in combination with an additional intermediate attachment or observation method. * 2 Require an additional intermediate attachment or fluorescence illuminator.

* 3 Cannot be used with U-TTLBI. * 4 Compatible with F.N. 22. * 5 Cannot be used with BX3-URA. * 6 Stand is a standard equipment of the U-MDOSV and U-MDO10R3. * 7 An auxiliary lens is equipped.

U-P4RE

Centerable revolving nosepiece

U-5RE-2

Quintuple revolving nosepiece

U-LHEAD* 5

Extension adapter for lamp housing

U-DULHA

Double lamp house adapter

C

A

U-AW

Motorized attenuator wheel

U-LH75XEAPO

75 W xenon apo lamp housing

U-LH100HGAPO

100 W mercury apo lamp housing

U-LH100HG

100 W mercury lamp housing

U-RFL-T

LIFE TIME

BURNER ON

U-RX-T

Power supply unit for xenon lamp

U-RFL-T

LIFE TIME

BURNER ON

U-RFL-T

Power supply unit for mercury lamp

U-ECA

Magnification changer 2x

U-ECA1.6x

Magnification changer 1.6x

U-ANT

Analyzer for

U-KPA transmitted light

Intermediate attachment for simple polarizing observation

U-CA

Magnification changer

U-DP* 1 * 3

Dual port

U-DP1xC

Dual port 1x

U-APT

Arrow pointer

U-EPA2

Eyepoint adjuster

U-EPAL-2

Eyepoint adjuster

U-TRU* 1 * 3

Trinocular intermediate unit

U-TRUS* 1

Trinocular intermediate unit

U-CPA

Intermediate attachment for conoscopic andorthoscopic observation

U-AN360P-2

Rotatable analyzer

U-OPA

Intermediate attachment for orthoscopic observation

U-DAL10X

Drawing attachment 10X

U-DA

Drawing attachment

U-DO3

Dual observation attachment

U-SDO3

Side by side observation attachment

U-MDOSV* 6

Multi observation side viewer

U-MDOB3

Multi observation body

U-MDO10B3

Multi observation body for 10 persons

U-MDO10R3* 6

Multi observation body for 10 persons

U-LC* 7

Low magnification condenser

U-AC2

Abbe condenser

U-SC3

Swing-out condenser

U-AAC

Achromatic/

Aplanatic condenser

U-ULC-2

Ultra low condenser

U-PCD2

Phase/darkfield condenser

U-POC-2

Polarizing condenser

Optical devices

U-CO1.25x

Low magnification conversion lens for UCD

U-UCDTP530

Tint plate for sensitive color observation

A

BX3-UCD8A

Motorized universal condenser

U-UCD8-2

8-position universal condenser

U-TLO

Oil top lens

U-TLD

Dry top lens

U-DCD

Darkfield condenser, dry

U-DCW

Darkfield condenser, oil

B

A

U-CBS

Control box for coded function

U-IFCBL200

Interface cable,

200 cm

U-HSEXP

Hand switch for exposure

U-HSCBM

Hand switch for CBM

U-CBM

Control box

PC (Software)

Stand* 6

DP21-CB

Control box

23 24

BX63 specifications

Microscope frame

Revolving nosepiece

Observation tube

Stage

Condenser

ND filter wheel

Fluorescence illuminator

Optical system

Focus

Fluorescence light source

Controller

Illuminator

Widefield (F.N. 22)

UIS2 optical system

Built-in motorized nosepiece focus

Stroke: 20 mm, minimum increment: 0.01 µm, maximum nosepiece movement speed: 3 mm/s

Built-in Koehler illumination for transmitted light, Light intensity LED indicator, Built-in motorized field stop

• High color reproductivity LED light source •12 V 100 W halogen bulb (pre-centered)

• Motorized septuple revolving nosepiece • Interchangeable reversed coded septuple nosepiece

• Widefield tilting trinocular • Widefield trinocular • Widefield erect image trinocular

• Widefield tilting binocular • Widefield tilting,Telescopic,Lifting binocular tube • Widefield ergo binocular

• Widefield binocular

• Ultrasonic stage (Stage stroke: X:76 mm x Y: 52 mm, maximum stage movement speed: 30 mm/s

• Ceramic-coated coaxial stage with left or right hand low drive control: with rotating mechanism and torque adjustment mechanism, optional rubber grips available

• Cross stage with short left handle

• Motorized universal condenser (N.A. 0.9, motorized 8-position turret, Aperture stop, polarizing filter in/out mechanism and top lens swing out mechanism), for 1.25x–100x [swing-out 1.25x-4x, with oil top lens: (N.A. 1.4)]

• Swing out Achromatic (N.A. 0.9), for 1.25x–100x (swing-out: 1.25x–4x)

• Achromatic Aplanatic (N.A. 1.4), for 10x–100x

• Universal (N.A. 0.9), for 1.25x–100x [swing-out: 1.25x–4x, with oil top lens:(N.A. 1.4)]

• Ultra low (N.A. 0.16), for 1.25x–4x

• Darkfield dry (N.A. 0.8–0.92), for 10x–100x

• Darkfield oil (N.A. 1.20–1.40), for 10x–100x

• Motorized 6-position ND filter wheel

• Motorized multi-purpose coded type (F.N. 22, motorized 8-position mirror unit turret, 4-position ND slider)

• Multi-purpose coded type (F.N. 22, 8-position mirror unit turret, 4-position ND slider)

• 100 W Hg apo lamp housing and transformer

• 100 W Hg lamp housing and transformer

• 75 W Xe lamp housing and transformer

• High-performance control box ( I/F: FireWire)

*This device is designed for use in industrial environments for the EMC performance (EC61326-1 Class A device). Using it in a residential environment may affect other equipment in the environment.

BX53 specifications

Microscope frame

Revolving nosepiece

Observation tube

Stage

Condenser

Fluorescence illuminator

Optical system

Focus

Illuminator

Widefield (F.N. 22)

Super widefield (F.N. 26.5)

Fluorescence light source

UIS2 optical system

Vertical stage movement: 25 mm stage stroke with coarse adjustment limit stopper, Torque adjustment for coarse adjustment knobs, Stage mounting position variable, High sensitivity fine focusing knob (minimum adjustment gradations: 1 µm)

Built-in Koehler illumination for transmitted light, Light preset switch, Light intensity LED indicator, Built-in filters (LBD-IF, ND6,

ND25, optional), 12 V 100 W halogen bulb (pre-centered)

Interchangeable reversed quintuple/coded quintuple/sextuple/septuple/coded septuple nosepiece

• Widefield tilting, telescopic and lifting binocular

• Widefield tilting trinocular • Widefield trinocular • Widefield erect image trinocular • Widefield tilting binocular

• Widefield tilting,Telescopic,Lifting binocular tube •Widefield ergo binocular •Widefield binocular

• Super widefield trinocular • Super widefield erect image trinocular

Ceramic-coated coaxial stage with left or right hand low drive control: with rotating mechanism and torque adjustment mechanism, optional rubber grips available (Non stick grooved coaxial, plain, rotatable stages are also available)

• Abbe (N.A. 1.1), for 4x–100x

• Swing out Achromatic (N.A. 0.9), for 1.25x–100x (swing-out: 1.25x–4x)

• Achromatic Aplanatic (N.A. 1.4), for 10x–100x

• Phase contrast, darkfield (N.A. 1.1), [phase contrast: for 10x–100x, darkfield: for 10x–100x (up to N.A. 0.80)]

• Universal (N.A. 0.9), for 1.25x–100x [swing-out: 1.25x–4x, with oil top lens:(N.A. 1.4)]

• Low (N.A. 0.75), for 2x–100x(Dry)

• Ultra low (N.A. 0.16), for 1.25x–4x

• Darkfield dry (N.A. 0.8–0.92), for 10x–100x

• Darkfield oil (N.A. 1.20–1.40), for 10x–100x

• Multi-purpose coded type (F.N. 22, 8-position mirror unit turret, 4-position ND slider)

• Economical type (F.N. 26.5, 8-position mirror unit turret)

• 100 W Hg apo lamp housing and transformer

• 100 W Hg lamp housing and transformer

• 75 W Xe lamp housing and transformer

*The U-CBM is designed for the BX3 use in industrial environments for the EMC performance (IEC61326-1 Class A device). Using it in a residential environment may affect other equipment in the environment.

BX63/BX53 common specifications

Operating environment • Indoor use

• Ambient temperature : 5 º to 40 ºC (41 º to 104 ºF)

• Maximum relative humidity : 80 % for temperatures up to 31º C (88 ºF), decreasing linearly through 70 % at 34 ºC

(93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)

• Supply voltage fluctuations : Not to exceed ±10 % of the normal voltage

BX63 FL dimensions

220 212.5*

(unit: mm) Touch panel controller dimensions (unit: mm)

FS AS

OFF MAX

ECO mode

FL SHUTTER

OPEN CLOSE

OBSERVATION

FL PH DIC BF PO DF

FL MIRROR UNIT

1 2 3 4 5 6 7 8

OLYMPUS

25

°

TURRET POL.

TOP LENS AS

OFF

ECO mode

MAX

FL SHUTTER

OPEN CLOSE

3

DF

UNIT

8

190

294.5

152.5

429

656

680.5

Weight: Approx. 35 kg, Power consumption: Approx. 440 W

The length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62 mm.

BX53 FL dimensions

175 184*

(unit: mm)

Weight: Approx. 2.4 kg, Power consumption: Approx. 25 W

BX3-CBH dimensions

210.5

(unit: mm)

SHUTTER

FS AS

100 280

294.5

Weight: Approx. 4.2 kg, Power consumption: Approx. 230 W

90

274.5

91.5

362

436.5

521.5

Weight: Approx 21 kg, Power consumption: Approx. 290 W

The length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62 mm.

BX53 BF dimensions (unit: mm)

175 184*

90

274.5

91.5

362

436.5

Weight: Approx. 16 kg, Power consumption: Approx. 140 W

The length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62 mm.

25 26