Olympus CH30, CH40 biological microscope Instruction manual

OLYMPUS
N %
INSTRUCTIONS
CH30/CH40
BIOLOGICAL MICROSCOPE
This instruction manual is for the Olympus Biological Microscope Models CH30/CH40. To ensure
the safety and obtain optimum performance and to familiarize yourself fully with the use of this
microscope, we recommend that you study this manual thoroughly before operating the micro-
scope. Retain this instruction manual in an easily accessible place near the work desk for future
reference.
AX9830
_ IMPORTANT
x 1. Make sure that the voltage selector switch (1) located on the underside
of the base of the microscope frame is set to conform with the local
mains voltage. Move the switch using an Allen wrench, etc. (Fig. 1)
O When shipped from the factory, the switch is set to 110-120V and
230-240V in 100V and 200V areas, respectively.
2. To avoid potential shock hazard and fire, always switch the main switch
O to “O” (OFF) and disconnect the power cord from the wall outlet
before replacing the bulb. Allow the lamp housing cover (3) and the bulb
to cool before touching.
3. Install the microscope on a level table. The air vents on the underside of
the base should never be blocked such as by placing the microscope on
a flexible surface, e.g., a carpet, etc., as this could result in overheating
and cause a fire.
4. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD" at the end of
this instruction manual. If the proper power cord is not used, Olympus
can no longer warrant the electrical safety and performance of the
equipment.
5. Connect the power cord correctly and ensure that the ground terminal
of the power supply and that of the wall outlet are properly connected.
If the equipment is not grounded, Olympus can no longer warrant the
electrical safety and performance of the equipment.
6. Never insert metal objects, etc. into the air vents of the microscope
frame as this will result in electrical shock and/or personal injury.
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols, and always use the
microscope in the safest possible manner.
Indicates that the surface becomes hot, and should not be touched with bare
hands.
Before use, carefully read the instruction manual. Improper handling could
result in injury to the user and/or damage to the equipment.
Indicates that the main switch is ON.
O— > E
Indicates that the main switch 1s OFF.
1. A microscope is a precision instrument. Handle it with care
and avoid subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct
sunlight, high temperature and humidity, dust or vibrations.
(For operating environment conditions, refer to and adhere to
the conditions specified in Section 6, SPECIFICATIONS on
page 16.)
3. The tension of the coarse focus adjustment knob should only
be adjusted by means of the tension adjustment ring.
O 4. Heat from this microscope 1s led away by natural convection.
Consequently, do not use it in a constricted space and ensure
that the room is well ventilated.
5. When moving the microscope, carry it with one hand under
the base (1) and the other hand holding at the recessed handle
on the rear of the arm @ as shown in the illustration on the
left. Handle the microscope with care when moving it.
* When moving the microscope, never hold the stage or
binocular section of the observation tube, etc. Also
make sure that eyepieces, specimen, etc. do not fall off.
* Sliding the microscope on the surface of the table may
damage or tear off the rubber feet and/or scratch the
table top surface.
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze
slightly moistened with a mixture of ether (70%) and alcohol (30%).
* Do not use a mixture of ether (70%) and alcohol (30%) to clean the lower lens (made of optical plastic) of
the eyepiece (NCWHK10X) because such solvents cloud the lens. If dust adheres to the lens, blow it off or
wipe it away gently with a dry cloth.
Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure
to keep these chemicals away from open flames or potential sources of electrical sparks — for example,
electrical equipment that is being switched on or off. Also remember to always use these chemicals only
in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the non-optical components of the microscope. To clean these,
use a lint-free, soft cloth lightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope as malfunction or damage may occur.
4. When not using the microscope, keep it covered with the provided dust cover.
Warning labels are affixed at parts where special precaution is required when handling and using the microscope.
Always heed the warnings.
[Caution for |
Warni bel Positi |
Г arning Label Position Base Underside Bulb Replacement]
If warning labels become soiled, peel off, etc. contact your local Olympus representative to have them replaced.
If the microscope is operated in a manner not specified by this manual, the safety of the user may be imperiled. In
addition, the equipment may also be damaged. Always operate the equipment as outlined in this instruction manual.
CH30/CH40
2-1 Assembly Diagram eee 2
2-2 Detailed Assembly Procedure... eerie eee 3
5-2 Stage essen eee eer rere earner eae 10
5-3 Observation TUDE .…..….................cvrrersrrenseensencennre mere carre sare nee ne sec m0 me 11
5-4 Condenser .............e...eseíesceeeiccerrecerenecenienorererene rare sens sante ne nee ace nee 12
5-5 fFocusing Adjustment Knobs ...................mexeererecercecenrencenee nene 13
5-6 Immersion Objectives cocina reas 13
5-7 SPhotomicrography cuir errr eee sae 14
5-8 Phase Contrast Attachments...........e...e. ee. .ee=eeereereeeriee re A 16
Ш PROPER SELECTION OF THE POWER SUPPLY CORD............. 22
CH30/CH40
* NCWHK10X (CH30)
* WHK10X (CH40)
JANLYIDNANON в
Observation Tube
* CH3-Bl45
* CH3-TR45
* CH3-M045
I Microscope Frame
* СНЗОВЕ
* CH3OLF
* CH40RF
* CH40LF
* NDA 4X, 10X, 40X, = Sof
100X oil CY)
= 2
Iris
man
Condenser |
CH3-CD
CH2-PCD
BH2-PC
BH-DCD
CH3-CDP
ASSEMBLY [NG]
The diagram below shows how to assemble the various components. The numbers indicate the order of assembly.
* When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
* Retain the provided Allen wrench. It should be used when units are replaced or added.
Eyepiece
Cord rest
(optional for CH30/CH40)
\
A
Objective
Power cord
Microscope frame
7
Condenser
Field iris diaphragm
attachment
(optional for CH30)
Blue filter
e
Compensating lens
(optional for CH30)
Filter holder (for CH30)
6V, 20W halogen bulb
(for CH30) e
ESP
6V, 30W halogen bulb
(for CH40)
/ 7g TED
[/
1. Turn the microscope frame on its side and pull the lamp housing knob
(D on the underside of the base to open the lamp housing cover.
2. Holding the halogen bulb (2) contained in the polyethylene bag to avoid
leaving fingerprints on the bulb, fully insert the contact pins into the
bulb socket 3). When properly seated, pull off the polyethylene bag.
{ Applicable Bulb )
CH30: 6V, 20W Halogen bulb: 6V20W HAL (Philips 7388)
CH40: 6V, 30W Halogen bulb: 6V30W HAL (Philips 5761)
Á Always use the designated bulb. Use of improper bulb may
result in fire.
A Do not touch the bulb with bare hands. If fingerprints are
accidentally left on the bulb, wipe the bulb with a soft, lint-free
cloth moistened with alcohol. Using a contaminated bulb will
shorten the service life of the bulb.
3. With the lamp housing knob still pulled out, close the lamp housing
cover. Then push in the lamp housing knob to lock the cover.
% The lamp housing cover cannot be closed if the knob is pushed
in before you attempt to close cover.
| Precautions for Halogen Bulb Replacement
A Whenever you replace the bulb during use or right after use,
first move the main switch to “O” (OFF), disconnect the power
cord from the wall outlet, and allow the bulb and parts around
the bulb to cool before touching.
% If the bulb burns out during an observation and needs to be
replaced, remove eyepieces, specimen, and other objects likely
to fall off, before tilting the microscope frame to replace the
bulb.
ea
1. Using the provided Allen wrench, loosen the observation tube
clamping screw (1).
2. Insert the circular dovetail mount at the bottom of the observation
tube into the opening on the microscope frame, positioning the
observation tube to point the binocular eyepieces towards the front.
Clamp the observation tube by tightening the clamping screw M.
AT1ANISS Y N
High
magnifi-
cation
Low
magnification
Fig. 5
Fig. 6
| Binocular Tube (CH3-BI45), Monocular Tube (CH3-MO45)
1. Remove the eyepiece dust cap (1). (Fig. 3)
2. Insert the eyepiece (2) into the eyepiece sleeve as far as it will go.
(Fig. 3) |
3. Mount the other eyepiece in the same manner.
г - | Using Eyepiece Micrometer Discs (optional) | = = = = = — — — 1
Eyepiece micrometer discs can be inserted into the NCWHK10X
eyepieces. Use 10 mm/100 micrometer discs.
1. Unscrew the micrometer mounting frame @) from the eyepiece
by turning counterclockwise.
2. Place a micrometer disc into the frame. The engraving on the
micrometer disc should face downward into the micrometer
mounting frame 3). (Fig. 4)
3. Screw the micrometer mounting frame back into the eyepiece
tube. (Fig. 4)
XxX Use 19 mm diameter micrometer discs.
Mount the objectives on the revolving nosepiece in such a manner
that the magnification increases from low to higher powers in a
counterclockwise direction.
O If there are empty sockets with no objective mounted, make sure to
attach the provided dust plugs.
1. Turn the coarse adjustment knob (1) to raise the stage to its highest
position.
2. Turn the condenser height adjustment knob (2) to lower the condenser
holder to the position where the clamping screw (9) can be turned.
3. Insert the condenser @ into the mounting hole 6) and clamp by
tightening the clamping screw (). Positioning the condenser with the
diaphragm lever (6) at the front facilitates operation.
4. Turn the condenser height adjustment knob (2) to raise the condenser
holder to its highest position.
CH30/CH40
O The blue filter converts the color temperature of tungsten illumination
into the color temperature of daylight to show the specimen in natural
colors.
Place the 32.5 mm diameter blue filter © in the filter holder M or the
compensating lens.
1. Insert the filter holder or the compensating lens () into the condenser
from below until it seats with click.
2. When mounting the compensating lens, place the lens so that the
centering screws (2) are at the front.
Holding the field iris diaphragm attachment (2) over the light glass exit
M with the filter holder notches ® positioned as shown in Fig. 9,
insert the attachment into the light glass exit opening until it seats
with a click.
* Attempting to insert the attachment with the notches (3) facing
in other directions than shown in Fig. 9 may break the mount-
ing clip.
A Cables and cords are vulnerable when bent or twisted. Never
subject them to excessive force.
A Make sure that the main switch O is on “O” (OFF) before
connecting the power cord. (Fig. 10)
Á Always use the power cord provided by Olympus.
If no power cord is provided, please select the proper power
cord by referring to the section “PROPER SELECTION OF THE
POWER SUPPLY CORD” at the end of this instruction manual.
AT9W3SSV N
ASSEMBLY №
1. Connect the power cord plug ©) 10 the AC receptacle 3). (На. 11)
2. Plug the power cord plug (9) into a wall outlet. (Fig. 12)
A Connect the power cord correctly and ensure that the ground
terminal of the power supply and that of the wall outlet are
properly connected.
If the equipment is not grounded, Olympus can no longer war-
rant the electrical safety and performance of the equipment.
A
)
© When the cord rest (CH3-CH) is attached on the rear panel of the
microscope frame, the power cord can be wound around it for
storage.
Align the hooks (2) on the cord rest (1) with the air vent slits at (3),
and insert the hooks into the slits until the cord rest is pressed against
the rear panel of the microscope frame. Then pull the cord rest down
to secure it.
Note: The illustration of the cord rest only shows the sections relevant
for the explanation here. The actual cord rest looks somewhat
different.
* Do not grasp the cord rest when moving the microscope. The
cord rest may suddenly come loose and you may drop the
microscope causing material damage or personal injury.
Detaching—Move the microscope frame to the edge of the table @).
Then apply a screwdriver (5) or Allen wrench to the lower portion of
the cord rest (1) and push in the [1] 2] directions to move the entire
cord rest upward to allow it to be detached.
CONTROLS
Interpupillary distance adjustment scale
Diopter
adjustment ring
Specimen holder Main switch
Light intensity
knob
Y-axis knob
X-axis knob
Aperture iris diaphragm knob
Filter holder For 45 mm
diameter filters Fine focus
adjustment knob
_CH30/CH40
Coarse focus
adjustment knob
Coarse adjustment
knob tension
adjustment ring
*Field iris diaphragm dial
*The CH30 is not equipped with the field iris diaphragm function but an optional field iris
diaphragm attachment can be mounted.
STOHLINOD a
SUMMARY OF OBSERVATION PROCEDURES
1. Switch the main switch to “| “ (ON) and adjust the brightness with the
light intensity knob. (Page 10)
2. Turn the revolving nosepiece to engage the 10X objective. Make sure
that the revolving nosepiece stops with an audible click.
3. Place a specimen on the stage. (Page 10)
4. Turn the X-axis knob (1) and Y-axis knob (2) to move the specimen into
the light path. (Page 11)
SUMMARY OF OBSERVATION PROCEDURES 5
5. Looking through the right eyepiece with your right eye, turn the coarse
adjustment knob to bring the specimen into focus.
After obtaining approximate focus, use the fine adjustment knob to
make fine adjustments.
6. Looking through the left eyepiece with your left eye, turn the diopter
adjustment ring to focus the specimen. (Page 11)
7. Adjust the interpupillary distance of the eyepieces. (Page 11)
8. Center the field iris diaphragm by manipulating the two centering screws
on the compensating lens. (CH40 only) (Page 12)
In case of CH30, centration is not necessary. If the optional field iris
diaphragm attachment is mounted, center the attachment in the same
manner as described above.
9. Engage the objective to be used for observation and adjust the light in-
tensity to the desired level, then readjust the focus.
10. Adjust the field iris diaphragm.
This only applies to the CH30 if the optional field iris diaphragm attach-
ment is mounted.
S3HNA3I00Hd NOILVAHISEO 40 AHVANNNS В
11. Adjust the aperture iris diaphragm. (Page 12)
USING THE CONTROLS a
10
USING THE CONTROLS
J
Fig. 16
=
— | { = |
Fig. 17
1. Switch the main switch (1) to “|” (ON).
2. Turning the light intensity knob (2) clockwise in the direction of higher
numbers makes the illumination brighter, and vice versa.
@ ricid iris Diaphragm (CH40 only) —
Using the field iris diaphragm dial (7, adjust the diameter of the field
iris for objective power to the extent that it just circumscribes the field
of view. When the field iris diaphragm is stopped down to circum-
scribe the field of view, it will exclude extraneous light and improve
image contrast within the field of view.
* When using 100X objective, the field iris diaphragm image will
not be visible within the field of view. Accordingly, stop down
the diaphragm to its smallest diameter.
© In case of CH30, the field iris diaphragm attachment is mounted on
the light exit glass of the microscope base.
(Fig. 17)
CEN specimen Placement
* Releasing the curved finger with great force, or suddenly re-
leasing your grip on the curved finger knob while releasing the
curved finger, will crack or damage the slide glass. Always
release the curved finger with great care.
| Specimen Holder for Single Slides |
1. Turn the coarse adjustment knob (1) counterclockwise in the direc-
tion of the arrow to lower the stage.
2. Open the spring-loaded curved finger 2) on the specimen holder and
slide the specimen slide into the specimen holder from the front.
3. After placing the slide as far as it will go, gently release the curved
finger O).
Specimen Holder for 2 Specimen Slides |
1. Place the first specimen slide as described in steps 1 and 2 above.
Then place the second specimen slide so that :t contacts the first
specimen slide.
2. Gently release the curved finger 2).
CH30/CH40
| Placing the Specimen Slide with One Hand
Place the specimen slide at the front of the stage, then slide the specimen slide on the stage surface to slowly and
gradually open the curved finger in the direction of the arrow. Insert the specimen slide into the specimen holder
until it is fully and properly seated in the specimen holder.
| Cover Glass |
Use cover glasses of 0.17 mm thickness in combination with objectives marked with the *160/0.17” engraving for
optimum performance of these objectives.
| Specimen Slide |
Specimen slides with a thickness between 0.9 mm and 1.2 mm are recommended for CH30/CH40 microscopes.
Using thicker specimen slides may prevent correct formation of the field iris diaphragm image on the specimen surface
and illumination will not be correct in case of darkfield observation.
О
: Turning the Y-axis knob (1) moves the specimen in the Y-axis direc-
© tion. Turning the X-axis knob (2) moves the specimen in the X-axis
direction.
* Never move the specimen by holding the specimen holder or
stage directly as this will damage the knob rotation mechanism.
* When further movement of the stage or specimen holder is
prevented by the provided limiting mechanism, the tension of
the Y-axis and X-axis knob rotation will increase. At this point,
do not rotate the knobs further. Applying excessive force could
Fig. 18 destroy the limiting mechanism.
(Fig. 19)
While looking through the eyepieces, adjust for binocular vision until
the left and right fields of view coincide completely.
Adjust so that the two index dots ® are horizontal.
© Placing the interpupillary distance indexes so that they are parallel
with the horizontal lines facilitates the adjustment.
Also keep the dots parallel with the horizontal lines in case of
interpupillary distances other than 50, 60, 70, and 75.
© Note your interpupillary distance so that it can be quickly duplicated.
1. Looking through the right eyepiece with your right eye, rotate the
coarse and fine adjustment knobs to bring the specimen into focus.
N 2. Looking through the left eyepiece with your left eye, turn the diopter
Fig. 20 adjustment ring (1) to focus the specimen.
SIOHLNO9 3HL ONISN a
11
USING THE CONTROLS В
12
Fig. 21
Field iris
diaphragm image
Eyepiece
field of view
Fig. 22
Aperture iris
diaphragm image
70-80%
30-20%
Objective pupil
Fig. 23
©
. With the 10X objective engaged and the specimen brought into focus,
turn the field iris diaphragm dial >) counterclockwise to stop down
the diaphragm somewhat. (Fig. 21)
. Turn the condenser height adjustment knob (2) to bring the field iris
diaphragm image into focus. (Fig. 21)
. While gradually opening the field iris diaphragm image, rotate the two
centering knobs 3) of the compensating lens to adjust so that the field
iris diaphragm image is centered in the eyepiece field of view.
(Figs. 21, 22)
. To check centration, open the field iris diaphragm until its image
touches the perimeter of the field of view. If the image is not precisely
inscribed in the field of view, center again. (Fig. 22)
. When used for actual observation, open the field iris diaphragm until
its image just circumscribes the field of view.
When the compensating lens and field iris diaphragm attachment are
mounted on the CH30, center the diaphragm in the same manner as
described above.
© The aperture iris diaphragm determines the numerical aperture of the
illumination system. Matching the numerical aperture (1) о? the
illumination system with that of the objective provides better image
resolution and contrast, and also increases the depth of focus.
O Since the contrast of microscope specimens is ordinarily low, setting
the condenser aperture iris diaphragm to 70-80% of the N.A. of the
objective in use is usually recommended. When necessary, adjust the
ratio by removing the eyepiece and looking into the eyepiece sleeve
while adjusting the aperture iris diaphragm lever @ until the image
shown in Fig. 23 is seen.
J
© Focusing with the fine adjustment knob is not affected by this pre-
1. The coarse adjustment knob tension is preadjusted for easy use.
However, if desired, one can change the tension using the tension
adjustment ring ). Applying a flat-bladed screwdriver to any of the
grooves (2 on the circumference of the ring and turning the ring in
the direction of the arrow increases tension, and vice versa.
2. The tension is too low if the stage drops by itself or focus is quickly
lost after adjustment with the fine adjustment knob ®. In this case,
turn the ring (1) in the direction of the arrow to increase tension.
The pre-focusing lever ensures that the objective does not come in
contact with the specimen and simplifies focusing. After focusing on
the specimen with the coarse adjustment knob, turn this lever (1)
clockwise in the direction of the arrow to set a lower limit on coarse
adjustment movement.
focusing lever. Accordingly, after using the coarse adjustment knob
to lower the stage for changing specimens or applying immersion oil
(see Section 5-6), refocusing is easily accomplished by rotating the
coarse adjustment knob to reach the pre-focusing position, then
making fine adjustments with the fine adjustment knob.
* When not required, leave the pre-focusing lever unlocked.
S 1I0OHILNOO9 3HL ONISN В
* Be sure to use the provided Olympus Immersion oil.
1. Focus on the specimen with a low power objective.
2. Place a drop of immersion oll (provided) onto the specimen at the area
to be observed.
3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.
* Since any bubbles in the oil will impair the image, make sure
that the oil is free of bubbles.
a. To check for bubbles, remove the eyepiece and fully open the field
N and aperture iris diaphragms, then look at the exit pupil of the
Fig. 27 objective inside the observation tube. (The pupil should appear
round and bright.)
b.To remove bubbles, rock the nosepiece slightly to move the oil
A Caution on Using the Immersion Oil: immersion objective back and forth a few times.
If the immersion oil comes into contact with your © If the condenser marking shows a numerical aperture (NA) of 1.0 or
o ne Tolowing action. more, the number applies only when oil is present between the slide
"than 15 min.). glass and the top surface of the condenser. When oil is not present,
For skin: Wash with soap and water. the NA is about 0.9.
If the appearance of the eye or skin changes or 4. After use, remove oil from the objective front lens by wiping with
pain continues, please consult your doctor.
gauze slightly moistened with an ether (7 parts) / alcohol (3 parts)
mixture.
* Using too much alcohol can dissolve the lens adhesive.
©
13
Automatic photomicrographic system
P 0
Photomicro adapter L
MOM-L Automatic photomicrographic system
PM-20
Automatic exposure photomicrographic system
PM-10AK
Photo eyepiece
-) NFK
adapter
e NFK photo eyepiece
PM-ADF
| Trinocular observation
tube
CH3-TR45
© Use the trinocular observation tube CH3-TR45 for photomicrography
Xx Use a heat absorbing filter (45HA) when using the PM-10, PM-20, and PM-30 photomicrographic systems.
© The interpupillary distance adjustment procedure of the trinocular
observation tube (CH3-TR45) is different from that of the binocular
observation tube (CH3-Bl45). The interpupillary distance dovetail
slides (1) on the CH3-TR45 should be moved to make the adjustment.
Move the light path selector knob (2) to select the required light path.
USING THE CONTROLS a
© For normal observation, the light path selector knob should be pushed in.
For TV and still photomicrography, pull out the knob.
eyepieces, 80% for
TV/photomicrography
Pushed in 100% for binocular Observation of dark
eyepieces specimens
Pulled out 20% for binocular Observation of bright
specimens,
photomicrography,
TV observation
* In case of long time exposure, ambient light in the room will
enter through the observation tube and the eyepieces and may
create ghost images or flare. To block this extraneous light, dim
the room or cap the eyepieces or the focusing telescope.
N a AL AL Bana A
[ Photomicrographic system | el a Mounting the Camera Unit „о (Fig.29)
y 1. Loosen the clamping screw @ of the NFK photo eyepiece adapter
||
(PM-ADF) ©.
2. Attach the NFK photo eyepiece adapter (PM-ADF) (1) to the trinocular
observation tube (CH3-TR45) (® and tighten the clamping screw (2).
3. Insert the NFK photo eyepiece (3.3X or 5X) @ into the NFK photo
eyepiece adapter (PM-ADF) (1).
4. Mount the camera unit directly on the NFK photo eyepiece adapter
(PM-ADF) M.
Using an OM Camera Back |
To use an OM camera back, mount the photo eyepiece adapter PM-
ADF (@) (into which a choice of NFK photo eyepiece is inserted), the
photomicro adapter MOM-L () and the OM camera back on the photo
tube in the described order.
e Use the 3.3X or 5X NFK photo eyepiece.
* Focus using the finder of the camera back.
e Photomicrographic magnification
= Objective magnification X Photo eyepiece magnification.
* To take a good photomicrography with a single lens reflex
camera back, a shutter speed at 1/2 second or slower is rec-
ommended so as to reduce the mirror-lockup shock.
The focus of the eyepieces and the camera film plane are not the
same. Consequently, always perform camera focusing by using the
focusing telescope of the camera unit. For details, refer to the instruc-
tion manual of the photomicrographic system.
EN Color Temperature Compensation (Fig.30)
© When using daylight type film in the camera.
% The blue filter is solely designed for visual observation (day-
| light). If this filter is engaged while taking photographs with
== Al 1. Mount the light balancing fiter (45LBD-2N) (D in the fier holder @
NN | => . Mount the light balancing filter - in the filter holder
El À of the microscope frame.
— А 2. Turn the light intensity knob 3). To obtain illumination close to day-
<< © light, set the index of the knob at 5 in case of the CH30, and 4 in the
N case of CH40.
Fig. 30
SIGLNO9 IHL ONISN В
15
USING THE CONTROLS a
| Light Annuli |
CH2-RS10
Centering knob
| Green Filter |
Centering knob
45G533
CH2-RS40
| Phase Contrast Objectives PCDA10 X PL/PCDA40X PL
Centering Telescope CT-5 |
Mount the phase annulus (CH2-RS10/CH2-RS40) in the same way as the filter holder.
* Replace previously mounted objectives with the phase contrast objectives.
\
(О Before centration After centration
a
b
a=b=c=d
Fig. 31
NN
N
. Turn the revolving nosepiece to move the objective with the same
number as the mounted light annulus.
. Set the specimen on the stage and bring it into focus.
. Remove either one of the eyepieces and insert the centering
telescope (CT-5) into the eyepiece tube.
. Rotate the CT-5's upper lens assembly so that the bright ring (1) (light
annulus in the condenser) and the dark ring @ (phase annulus in the
objective} come into focus. (Fig. 31)
. Rotate the two light annulus centering knobs @) until the light annulus
and phase annulus are concentric and superimposed. {Figs. 31 & 32)
. Remove the CT-5 and insert the eyepiece back into the eyepiece tube
to perform phase contrast observation.
. To increase image contrast, mount the filter on the filter holder.
| ESTI TE
EMO °
Optical system
LB optical system (finite-corrected system)
Hlumination llluminator built-in
6V 20 W halogen bulb Philips 7388 6V 30 W halogen bulb Philips 5761
(Average service time: Approximately 100 hrs. | (Average service time: Approximately 100 hrs.
when used as directed) when used as directed)
CH3ORF100/LF100: 100/110-120V, 50/60 Hz | CH40RF100/LF100: 100/110-120V, 50/60 Hz
0.3A 100-120V area 0.42A 100-120V area
CH3ORF200/LF200: 220/230-240V, 50/60 Hz | CH40RF200/LF200: 220/230-240V, 50/60 Hz
0.15A 220-240V area 0.21A 220-240V area
Focusing Stage movement by roller guide (Rack & Pinion)
Stroke per rotation: 39.6 mm
Fuli range stroke: 25 mM
Upper limit stopper
Torque adjustment on coarse focus knob
Revolving Build-on quadruple nosepiece Build-on quintuple nosepiece
nosepiece with inward tilt with inward tilt
Observation tube Monocular o о
CH3-MO45 Tube inclination 45
Binocular Tube inclination 45°
CH3-B145 Interpupillary distance adjustment 48-75 mm
Stage Size 188 mm x 134 mm
Movement 76 mm X-direction X 50 mm Y-direction
Specimen holder Double slide holder
Condenser Type Abbe condenser
N.A. 1.25 (with oil immersion)
Aperture iris diaphragm Provided
Dimensions and weight 233(W) x 392(H) x 301(D) mm; 8.2 kg (18 Ib) (CH30)/8.5 kg (18.7 Ib) (CH40)
Operating e Indoor use
environment e Altitude: Max. 2000 m
e Ambient temperature:
5° to 40°C( 41° to 104°F)
e Maximum relative humidity 80% for temperatures up to 31°C (88°F) decreasing linearly
through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative
humidity at 40°C (104°F)
e Main supply voltage fluctuations not to exceed £10% of the nominal voltage.
e Pollution Degree
2 (in accordance with IEC 664)
e Installation/Overvoltage Category {in accordance with IEC 664)
SNOILLVOI3IOIdS о
17
_ OPTIC pp yy y normar o
x OPTICAL CHARACTERISTICS
18
Objective designation ns“
| oumeus Da
Numerical aperture
И NDA
Magnification | oxoes |
—_— 160017 —1 Cover glass thickness
Mechanical tube length Color band
— Color band
NDA 4X
0.10 28.89 3.4 40X 175 4.5
Achromat 10X 0.25 6.33 1.3 100X 28.0 1.8
40X 0.65 0.62 0.52 400X 3.04 0.45 | Spring-loaded
100X 1.30 0.20 0.26 1000X 0.66 0.18 | Spring-loaded
Oil immersion
CH40
NDA AX
Achromat 10X
40X
100X
0.10 28.89 3.4 40X 175 5.0
0.25 6.33 1.3 100X 28.0 2.0
0.65 0.62 0.52 400X 3.04 0.5 Spring-loaded
1.30 0.20 0.26 1000X 0.66 0.2 Spring-loaded
Oil immersion
* The resolving power of immersion objectives Is obtained with the aperture iris diaphragm fully open.
Working distance (W.D.):
Numerical aperture (N.A.):
Resolving power:
Focal depth:
Field number:
Field of view diameter:
Total magnification:
The distance from the cover glass surface to the nearest point of the objective.
The N.A. value represents a performance number which can be compared to the
relative aperture (f-number) of a camera lens. The higher N.A., the higher the resolving
power.
The ability to differentiate two points, i.e., the minimum distance by which the objects
must be separated in order to be revealed as two separate objects.
The depth in the image through which the focused image will appear uniformly sharp.
As the aperture iris diaphragm is stopped down, the focal depth becomes greater. The
greater the N.A. of an objective, the shorter the focal depth.
A number that represents the diameter in mm of the image of the field diaphragm
that is formed by the lens in front of it.
The actual size of the field of view in millimeters.
Equals the objective magnification multiplied by the eyepiece magnification.
A ETT,
Under certain conditions, performance of this unit may be adversely affected by factors other than defects.
if problems occur, please review the following list and take remedial action as appropriate. If you cannot solve the
problem after checking the entire list, please contact your local Olympus representative for assistance.
a. Field of viewis obscured, | Nosepiece 1s not correctly engaged. 8
or field of view Is not clicks properly into place.
evenly illuminated.
The condenser is not correctly attached. | Re-insert the condenser all the way 4
without tilt.
The field iris diaphragm is not properly | Center the field iris diaphragm. 12
centered. (CH40 only)
The field iris diaphragm is stopped down | Open the field iris diaphragm. 12
too far.
Dirt/dust on the objective, eyepiece, | Clean thoroughly. —
condenser, and/or light exit glass.
b. Dirt or dust is visible in | Dirt/dust on the light exit glass.
the field of view.
Dirt/dust on the top lens of the con-
denser. Clean thoroughly. —
Dirt/dust on the specimen.
Dirt/dust on the eyepiece.
C. The image shows The condenser 1s lowered too far. Adjust the condenser height position. —
diffraction.
The aperture iris diaphragm is stopped | Open the aperture iris diaphragm. 12
down too far.
d. Visibility is poor. The objective is not correctly engaged in | Make sure that the revolving nosepiece 8
e Image is not sharp. the light path. clicks into place correctly.
e Contrast is poor.
e Details are indistinct. | Front lens of the objective Is dirty. Clean the objective. —
Immersion oil is not being used with an | Use immersion oil. 13
Oil immersion objective.
The immersion oil contains bubbles. Remove bubbles. 13
Recommended immersion oil not used. | Use the provided immersion oil. —
Specimen 1s dirty. Clean. —
Eyepiece, condenser 1s dirty. Clean. —
e. Part of the image is The objective is not stopped at the click- | Make sure that the revolving nosepiece 8
blurred. stop. clicks into place correctly.
The specimen is not mounted correctly | Place the specimen correctly on top of 10 11
on the stage. the stage and secure it with the speci-
men holder.
f. The image has a yellow | The blue filter is not engaged. Engage the blue filter. 5
tint.
© J0IND ONILOOHSH19N0EL
19
o TROUBLESHOOTING GUIDE
20
a. The coarse adjustment
The tension adjustment ring 1s over-
Loosen the ring.
men before focus is
obtained.
knob is hard to turn. tightened. 13
You are trying to raise the stage with the | Unlock the pre-focusing lever.
coarse adjustment knob even though the 13
pre-focusing lever is locked.
b. The stage drifts down by | The tension adjustment ring is too loose. | Tighten the ring.
itself, or focus is lost 13
during observation.
c. Coarse adjustment will | The pre-focusing lever is keeping the stage | Unlock the pre-focusing lever. 13
not go all the way up. down.
d. Coarse adjustment will | The condenser holder is too low. Raise the condenser holder. —
not go all the way down.
e. The objective makes | The specimen is mounted upside-down. Mount the specimen correctly.
contact with the speci- 10.11
a. Field of view of one eye
does not match that of
the other.
The interpupillary distance is incorrect.
Adjust the interpupillary distance.
11
Incorrect diopter adjustment.
Adjust the diopter.
11
Different eyepieces are used on the left
and right.
Change one eyepiece to match the other
so that both sides are the same.
The optical axes are not parallel.
Upon looking into the eyepieces, try
looking at the overall field before
concentrating on the specimen range. You
may also find it helpful to look up and into
the distance for a moment before looking
back into the microscope.
Image blurs as you
move the specimen.
The specimen is not correctly positioned
on the stage.
Mount the specimen correctly.
Front lens of a high
power objective comes
into contact with speci-
men when it is engaged
after a low power objec-
tive.
Specimen is mounted upside-down.
Mount the specimen correctly.
10,11
Cover glass is too thick.
Use a 0.17 mm thick cover glass.
11
a. The bulb does not light.
Bulb is not mounted. Mount the designated bulb. 3
The bulb is burned out. Replace the bulb. 3
The power cord plug is not connected. Connect the power cord. 5,6
b. The bulb is dark. The voltage selector switch is set | Set the switch to match the local AC
Incorrectly. mains.
c. The bulb burns out almost | Wrong type of bulb used. Use the correct bulb type. 3
Immediately.
The voltage selector switch is set | Set the switch to match the local AC
incorrectly. mains.
—e
OPTIONAL UNITS
Brightfield/darkfield phase
contrast attachment
CH2-PCD
Centration of phase contrast
insert possibie.
Centration of field iris diaphragm
not possible.
Dry darkfield condenser
CX-DCD
Centration of darkfield field of
view possible by using the
centering knobs of the con-
denser.
Phase contrast attachment
BH2-PC
Centration of phase contrast
insert possible.
Centration of field iris diaphragm
not possible.
Simple polarizing light attachment
set CH3-POL
(Contains)
Condenser CH3-CDP
Analyzer CH3-AN
Polarizer U-POT
The CH3-POL is provided with
the CH40. Attaching the com-
pensating lens below the CH3-
CDP allows centration of the
field iris diaphragm.
* The standard condenser does
not allow polarized light
observation.
In case of CH30, centration of
the field iris diaphragm becomes
possible If the compensating
lens and field iris diaphragm
attachment are added.
CH30/CH40
© SLINN TVNOILJO
N
—
Hl PROPER SELECTION OF THE POWER SUPPLY CORD
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and
“ Certified Cord ” below:
CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the
electrical safety of the equipment.
Specifications
Voltage Rating
Current Rating
Length
Temperature Rating
Fittings Configuration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
GA minimum
60°C minimum
305 m maximum
Grounding type attachment plug cap. Opposite terminates in molded-on IEC con-
figuration appliance coupling.
A power supply cord should be certified by one of the agencies listed in Table 1, or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in
Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the
agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in
your country.
Table 1 Certified Cord
22
Argentina IRAM (№ italy IMQ ©
Australia SAA XY Japan MIT] Y
Austria OVE Go Netherlands KEMA
Belgium CEBEC Norway NEMKO
Canada CSA G- Spain AEE Q
Denmark DEMKO ©) sweden SEMKO (5)
Finland РЕ (© Switzerland SEV ©
France UTE “Kino om AA S &
Germany VDE USA UL
Ireland NSA <>
APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Table 2 HAR Flexible Cord
| prio plectroteannique Belge CEBEC «НАБУ 10 30 10
NO Deutscher Elektrotechniker VDE HAR, 30 10 10
oe Technique de IElectricite” USE HAR 30 10 30
DUO ane det Marehio df EMMEQU HAR 10 30 50
Ces (ASE Service for Electric BASEC HAR 10 10 30
NV. KEMA KEMA-KEUR HAR» 10 30 30
SEHK 10 Sete Eek emo A | |» |
Soro vp U VE HAR 30 10 5
DEMO Elektriske Materialkontroll ¿DEMKO> HAR 30 10 30
Ne Standards Authority of Ireland ¿NSAI> «НАБУ 30 30 50
NERO e Materiellkontroll NEMKO HAR 10 10 70
Be es we we | x | 20 | м
Standarizalon CLOT andl HAR 30 30 Ш
ra Portages da Qualidade np HAR 10 10 90
Món SE sev Me | 0 | | ®
Elektriska Inspektoratet SETI <HAR> 10 30 90
SV, SVT, SJ or SIT, 3 X 18AWG
SV, SVT, SJ or SIT, 3 X 18AWG
Underwriters Laboratories Inc. (UL)
Canadian Standards Association (CSA)
This device complies with the requirements of both directive 89/336/EEC concerning electromagnetic compatibility
and directive 73/23/EEC conceming low voltage. The CE marking indicates compliance with the above directives.
. 23
OLYMPUS
OLYMPUS OPTICAL CO.,LTD.
2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan
OLYMPUS OPTICAL CO.(EUROPA) GMBH.
Postfach 10 49 08, 20034, Hamburg, Germany
OLYMPUS AMERICA INC.
2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A.
OLYMPUS SINGAPORE PTE LTD.
491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373
OLYMPUS OPTICAL CO.(U.K) LTD.
2-8 Honduras Street, London EC1Y OTX, United Kingdom.
OLYMPUS AUSTRALIA PTY. LTD.
104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia
This publication is printed on recycled paper.
Printed in Japan 2002 01 M 200-®
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