Olympus BX 53 microscope Instruction manual

INSTRUCTIONS
BX53
SYSTEM MICROSCOPE
This instruction manual is for the Olympus System Microscope Model BX53.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
AX7855
In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user's authority to operate the equipment.
BX53
CONTENTS
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 10, “ASSEMBLY" (pages 34 to 37) carefully.
IMPORTANT
-- Be sure to read this section for safe use of the equipment. --
1-3
1
MODULE NOMENCLATURE
2
CONTROLS
5-7
3
FLOW OF OBSERVATION
8,9
4
SIMPLIFIED OBSERVATION PROCEDURE
4
10-12
4-1 Basic Operation (Until Observation of Specimen)........................................................................10,11
4-2 Microscope Adjustments (How to Improve the Observed Image) ........................11,12
5
1 Adjusting the Interpupillary
Distance ................................................................................. 11
2 Adjusting the Diopter ........................................ 11
3 Adjusting the Centering ..................................12
4 Adjusting the Contrast ......................................12
USING THE CONTROLS
13-26
5-1 Base ...............................................................................................................................................................................................................13-16
1 Adjusting the Brightness ...............................13
2 Using the Light Intensity Preset
Switch........................................................................................13
3 Using the ECO/NORMAL Switch .......14
4 Using the Filters............................................... 14-16
5-2 Focusing Block ................................................................................................................................................................................. 17,18
1 Focusing Controls ................................................... 17
2 Adjusting the Focus .............................................. 17
3 Replacing the Fine Adjustment
Knob ........................................................................................... 18
5 Pre-focusing Lever ................................................. 18
4 Adjusting the Coarse Adjustment
Knob Tension................................................................. 18
5-3 Stage.............................................................................................................................................................................................................19-21
1 Placing the Specimen .......................................19
2 Using the Auxiliary Slide Holder..........19
3 Adjusting the X- and Y-Axis Knob
Tension ................................................................................. 20
5 Adjusting the Stage Height ..................... 21
4 Rotating the Stage ..................................... 21
5-4 Observation Tube ................................................................................................................................................................... 22-24
1 Adjusting the Diopter ....................................... 22
2 Using the Eye Shades.................................... 22
3 Using the Eyepiece Micrometer
Disk ............................................................................................ 23
4 Selecting the Light Path of the
Trinocular Tube ......................................................... 23
6 Using Eyepieces Incorporating a
Micrometer ...................................................................... 25
5 Adjusting the Tilt ..................................................... 24
5-5 Condenser ................................................................................................................................................................................................... 25
1 Compatibility of Objectives and
Condensers ................................................................... 25
5-6 Immersion Objectives ................................................................................................................................................................. 26
1 Using Immersion Objectives................. 26
5-7 Objectives with Correction Collar .............................................................................................................................. 26
6
CAMERA RECORDING
7
TROUBLESHOOTING GUIDE
8
SPECIFICATIONS
9
OPTICAL CHARACTERISTICS (UIS2 Series)
10
ASSEMBLY -- See this section for the replacement of the light bulb. --
11
LAMP HOUSING INSPECTION SHEET
27
28-30
31,32
33
34-37
38
n PROPER SELECTION OF THE POWER SUPPLY CORD .......................................................... 39,40
BX53
IMPORTANT
This microscope employs a UIS2 (Universal Infinity System) optical design, and should be used only with
UIS2 eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX
series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus
or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.
SAFETY PRECAUTIONS
(Fig. 1)
1. If potentially infectious samples may be observed, use protective gloves or
other protective means to prevent the skin from contacting with samples
directly.
After observation, be sure to clean the portion contacted with samples.
· Moving this product is accompanied with the risk of dropping the
sample. Be sure to remove the sample before moving this product.
· In case the sample is damaged by erroneous operation, promptly take
the infection prevention measures.
· Follow the procedures described in Chapter “Getting Ready” (see on
page 2) prior to using the accessories of this product. Otherwise, the
stability of the device will be lost and the dropped sample will cause
the possibility of infection.
· When you maintain the device which may have contacted with potentially infectious reagents, be sure to wear the protectors such as gloves,
or clean the device prior to operation.
· Before disposing of device contacted with potentially infectious samples,
be sure to follow the regulations and rules of your local government.
2. Install the microscope on a sturdy, level table or bench.
3. Be careful not to tilt the microscope too much. Otherwise, the mobile
parts such as the light path selector knob may move in unintended
directions.
4. The surfaces of the lamp housing on the rear of the microscope will
become extremely hot during operation. When installing the microscope,
make sure to allow ample free space (10 cm or more) around and in
particular above the lamp housing.
5. When installing the microscope, route the power cord away from the
lamp housing. Should the power cord come in contact with the hot lamp
housing, the power cord could melt and cause electric shock.
6. To avoid potential shock hazards and burns when replacing the light bulb,
” (OFF) then disconnect the power cord
set the main switch @ to “
from the wall outlet in advance. Whenever you replace the bulb during
use or right after use, allow the lamp housing, and bulb to cool before
touching. (Fig. 1)
1
Designated bulb
CAUTION
Fig. 1
12V100WHAL-L (PHILIPS 7724)
Always use the designated lamp bulb. Using other bulb may
cause malfunction.
7. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this
instruction manual. If the proper power cord is not used, product safety
performance cannot be warranted.
8. Always ensure that the grounding terminal of the microscope and that of
the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.
9. Never insert metallic objects into the air vents of the microscope frame
as this could result in electrical shock, personal injury and equipment
damage.
10. The standard service life of the lamp housing is eight (8) years of use or
20,000 hours of total power ON period, whichever is the shorter period.
For details, see Inspection Sheet on page 38.
1
Restrictions in Use
When all combinations (@ to ³) below are satisfied at the same time, the microscope may fall if tilted larger than a certain
degree, comparing to other combinations. Please mount the camera port of the intermediate observation tube toward
diagonally backward 45° to prevent from falling.
@ The observation tube is either the trinocular tube, U-TTBI, U-ETBI or U-TTLBI.
² The intermediate observation tube is either U-TRUS or U-DP.
³ The camera heavier than 0.4kg is mounted on the intermediate observation tube described in ².
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol
Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Indicates a non-specific general hazard. Follow the description given after this symbol or in
instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Caution indications
Caution labels are placed at parts where special precaution is required when handling and using the microscope. Always
heed the cautions.
Caution label
position
Lamp housing
[Caution against high temperature]
Caution label
position
Rear panel
[Caution against high temperature]
If a caution label is dirty or peeled off, contact Olympus for the replacement or other inquiry.
1
Getting Ready
(Fig. 2)
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also note that the microscope
does not have a waterproof construction.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions,
refer to section 8, “SPECIFICATIONS”.)
3. When moving the microscope, remove the observation and specimen
and carefully carry the microscope by holding the grasping part on the
rear of the arm and the front part of the base as shown in Fig. 2.
CAUTION
Fig. 2
2
To prevent damage to the microscope, never hold it by the stage
or coarse/fine adjustment knob.
· Even when moving the microscope along the desktop surface, be sure
to remove the specimen in advance.
· When the microscope needs to be packaged for forwarding to a remote
location, contact Olympus.
Please do not lift up the microscope by holding the bottom side of the
power supply cover (black) located on the rear panel.
4. When the accessories of this product are used, make sure that the system
height does not exceed 1 meter. Otherwise, the stability of the device will
be lost.
5. When an arm, illuminator, intermediate attachment, observation tube and
camera are to be attached to the upper part of the microscope frame,
make sure that their total weight does not exceed 18 kg.
BX53
2
Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
CAUTION
· Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical
equipment that is being switched on or off, which could cause ignition of a fire.
Also remember to always use it only in a well-ventilated room.
· Set the main switch to “ ” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding
to maintenance.
2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them, use
a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual,
as this could result in malfunction or reduced performance.
4. After using the microscope, set the main switch to “
” (OFF), wait for the lamp housing to cool down sufficiently, and
keep it covered with a dust cover during storage.
5. Before disposing of this product, be sure to follow the regulations and rules of your local government.
3
Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor
}
4
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.
: Indicates commentary (for ease of operation and maintenance).
Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
Do not use this product for any purpose other than its intended use.
This product is considered a medical device in the following country: US.
This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.
Safety and EMC Precautions
· This product complies with the emission and immunity requirements described in IEC61326 series.
· The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in
close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation.
· Use only power cord which OLYMPUS specifies. Otherwise the safety and EMC performance of the product can not
be assured.
· Be sure to ground the product. Otherwise our intended electric safety and EMC performance of the product can not
be assured.
3
1 MODULE NOMENCLATURE
} The modules mentioned below show only the typical product names. As there are some products that are not mentioned
but also applicable to this microscope, check the latest catalogues or consult Olympus.
For the products marked “ * ”, also read their instruction manuals.
Observation tube
· U-BI30-2
· U-TBI-3
· U-TR30-2
· U-TTLBI
Eyepieces
· WHN10X
· WHN10X-H
Intermediate attachment
· U-TRU
· U-CA
· U-DO3
Arm (or vertical illuminator)
· BX3-ARM
· BX3-URA*
· BX3-RFAS*
· BX3-RFAA*
Lamp housing
· U-LH100-3
Revolving nosepiece
· U-D6RE
· U-D7RE
· U-D7RES
· U-D7REA
Slide holder
· U-HLST-4
· U-HLD-4
· U-HLDT-4
Stage
· U-SVRB-4
· U-SVRO
Condenser
· U-SC3
· U-AAC
· U-UCD8
· BX3-UCD8A
4
BX53
2 CONTROLS
} If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 34 to 37).
Interpupillary distance adjustment
scale (Page 11)
Light path selector knob
(Page 23)
Condenser height adjustment
knob (Page 12)
Diopter adjustment ring
(Page 22)
Slide holder (Page 19)
Aperture iris diaphragm ring
(Page 12)
Filter mount (Page 15)
Field iris diaphragm ring
(Page 12)
Allen screwdriver
holder
Coarse adjustment
knob (Page 17)
Y-axis knob* (Page 20)
X-axis knob* (Page 20)
Light preset adjustment
screw (Page 13)
Coarse adjustment tension
adjustment ring (Page 18)
Filter knobs (Page 15)
ND6, ND25 and LBD from the front to
the rear (In-out possible from the other
side)
* The U-SHG (thin type) and U-SHGT (thick type) stage feed
knob rubber caps can be attached optionally.
5
Main switch (Page 10)
I : ON
: OFF
Pre-focusing lever
(Page 18)
Pilot LED
Fine adjustment knob
(Page 17)
Green lighting: Lamp bulb ON
Red lighting: Auto OFF
Detachable.
Human sensor (Page 14)
Light intensity preset switch
(Page 13)
Coarse adjustment knob
(Page 17)
Brightness adjustment knob
(Page 13)
Filter knobs (Page 15)
ND6, ND25 and LBD from the front
to the rear
(In-out possible from the other side)
ECO indicator (Page 14)
ECO: ON (Green)
NORMAL: OFF
6
ECO/NORMAL switch (Page 14)
BX53
Low Magnification Condenser
U-LC
Swing-out Condenser
U-SC3
Top lens
Auxiliary lens mounting
tool
Top lens swing-out
knob
Aperture iris diaphragm
ring (Page 12)
Auxiliary lens for U-LC
Condenser mount
dovetail
* The auxiliary lens is mounted on the lamp adapter when the U-LC is used. (Page 35)
7
3 FLOW OF OBSERVATION
} When the ECO mode is set, the halogen bulb turns OFF automatically in about 30 minutes the microscope becomes
unattended.
(Controls Used)
Set the main switch to “ I ” (ON) and adjust
the brightness.
Engage only the LBD filter.
Select the light path (trinocular tube).
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen in focus.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Adjust the aperture iris and field iris diaphragms.
Engage the objective to be used in the light
path and bring the specimen in focus.
Engage the required filters.
Adjust the brightness.
Start observation.
8
@Main switch
2Brightness adjustment knob
3LBD filter knob
(Page)
(P. 13)
(P. 10,14-16)
4Light path selector knob
(P. 23)
5Slide holder
6X-/Y-axis knobs
(P. 19)
(P. 20)
7Revolving nosepiece
8Coarse/fine adjustment knobs
(P. 17)
9Binocular tube
aDiopter adjustment ring
bCondenser height adjustment
knob
cCondenser centering knob
(P. 11)
(P. 22)
(P. 12)
dAperture iris diaphragm ring
eField iris diaphragm ring
(P. 12)
(P. 12)
7Revolving nosepiece
8Coarse/fine adjustment knobs
(P. 17)
fFilters
2Brightness adjustment knob
(P. 12)
(P. 10,14-16)
(P. 13)
BX53
9
4
1
b
a
7
5
d
6
c
e
3
2
f
8
} Copy the observation procedure pages on separate sheets and post it near your microscope.
9
4 SIMPLIFIED OBSERVATION PROCEDURE
4-1 Basic Operation (Until Observation of Specimen)
This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed
operating procedure of each control, please read the description page specified below.
1
Press the main switch of the microscope frame to “ I ” (ON).
2
Rotate the knob to adjust the lamp brightness. (Details: Page 13)
3
Push in the LBD filter knob to engage the LBD filter in the light path.
(Details: Page 15)
1
Fig. 3
2
Fig. 4
3
Fig. 5
What is LBD filter?
The color of the sun at the noon of a clear day is called the
daylight color, which looks white to human eyes. On the other
hand, the halogen bulb used as the light source of this microscope does not emit the daylight-color light, so the LBD filter is
engaged in the light path to convert the color of halogen bulb
light into daylight. In general, engaging the LBD filter makes it
possible to observe specimens in natural color tones.
10
BX53
5
4
Rotate the knob to lower the stage. (Details: Page 17)
5
Open the clamping lever of the slide holder and place the specimen
slide. (Details: Page 19)
6
Rotate the revolving nosepiece to engage the 10X objective in the
light path.
7
Rotate the coarse and fine adjustment knobs to bring the specimen
into focus. (Details: Page 17)
8
Rotate the stage knob to adjust the observation position.
4
Fig. 6
6
7
8
Now you can observe the magnified image of the specimen. To
improve the observation further, read section 4-2, “Microscope
Adjustments” below.
Fig. 7
4-2 Microscope Adjustments (How to Improve the Observed Image)
Adjust the microscope as described below to improve the observed image.
1
Adjusting the Interpupillary Distance
1
While looking through the eyepieces, adjust for binocular vision until
the left and right fields of view coincide completely.
} Note your interpupillary distance so that it can be quickly duplicated.
1
Fig. 8
2
Adjusting the Diopter
The diopter adjustment ensures parfocality from low to high magnifications and makes it possible to avoid defocusing even when the
objective is switched.
For details, see page 22.
Fig. 9
11
3
Adjusting the Centering
1
2
4
7
6
5
3
8
9
1
Place the specimen.
2
Rotate the revolving nosepiece to select the 10X objective.
3
Rotate the knobs to bring the specimen into focus.
4
Rotate the knob to raise the condenser to its upper limit.
5
Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field
of view.
6
Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 10).
7
Turn the two condenser centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 10).
8
Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 10).
9
Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 10).
} Be careful not to rotate the condenser clamping screw, located behind the centering knobs, by mistake.
} When using a condenser with top lens such as the U-SC3, engage the top lens in the light path before proceeding to
the centering adjustment.
A
B
Fig. 10
4
C
D
Movement of Field Iris Diaphragm Image
Adjusting the Contrast
Aperture iris
diaphragm image
70-80%
30-20%
Objective pupil
N.A. scale
12
2
1
1
Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the
objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.
2
The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation
tube. Adjust so that the image looks approximately like that shown in the figure above.
5 USING THE CONTROLS
BX53
5-1 Base
1
Adjusting the Brightness
(Fig. 11)
Turn the brightness adjustment knob @ clockwise to increase the voltage
and make illumination brighter.
} When the brightness adjustment knob @ is adjusted to the camera
symbol marking, the brightness of the light becomes the daylight (color
temperature of about 5500K). However, note that the daylight may not
always be reproduced accurately depending on the degree of bulb
consumption, etc.
1
Fig. 11
2
1
2
Fig. 12
Using the Light Intensity Preset Switch
(Fig. 12)
The light intensity preset switch @ makes it possible to limit the light
intensity to a preselected level regardless of the position of the brightness
adjustment knob.
The light intensity preset has been preset to optimum brightness for
photography when the built-in LBD filter is engaged (approx. 9 V with
marking).
1. Press the light intensity preset switch @ to the ON position. (The face of
the switch lights when it is ON.)
2. Using a small flat-blade screwdriver, turn the preset adjustment screw 2
to obtain the required light intensity. Turning the screw clockwise increases
brightness.
3. When the light intensity preset switch @ is set to OFF, the brightness
returns to the level set by the brightness adjustment knob.
} While the light intensity preset switch is ON, turning the light brightness
adjustment knob does not affect brightness.
13
3
5
4
(Fig. 13)
} The ECO mode helps prevent leaving the halogen bulb ON while the
microscope is not used.
1. Press the ECO/NORMAL switch 3 to ON (so that the PILOT lamp |
lights in green). Hereafter, when the human sensor 5 of the microscope
has not detected human motion, etc. for more than about 30 minutes,
the lighting halogen bulb is turned OFF automatically (the PILOT lamp |
lights in red).
2. To turn the bulb ON again after it has been turned OFF automatically:
· Set the main switch to “
” (OFF) then “ I ” (ON) again.
· Press the ECO/NORMAL switch 3 twice.
1
2
3
Using the ECO/NORMAL Switch
Fig. 13
} In the ECO mode, the human sensor may sometimes be unable to detect
a human body and the halogen bulb be turned OFF during use of the
microscope in the following cases.
· When the heat source (human skin, etc.) in the detection range of
the human sensor hardly moves or moves at a high speed.
· When far-IR rays from the sun or incandescent lamp is directly incident
on the human sensor.
· When an object that transmits little far-IR rays, such as glass or acrylic
plate, is located between the human sensor and the person operating
the microscope.
· When there is little difference between the ambient temperature and
the body temperature of the person.
} In the ECO mode, the halogen bulb may not be turned OFF automatically
in the following cases. In addition to these cases, the halogen bulb may
neither be turned OFF in the case of sensor detection error.
· When the sensor detects the movement of a heat source other than
human body.
· When the sensor detects a heat source that repeats heat generation,
such as a motor repeating ON-OFF.
· When the temperature in the detection area changes suddenly, for
example due to hot/cold winds of an air conditioner or vapor from a
room humidifier.
4
Using the Filters
(Figs. 14 to 20)
} You can place a filter in the light path with one of the following methods.
· Push in the knob of the desired built-in filter to engage it in the light
path. (Page 15)
· Place a filter on the filter mount on the base and engage in the light
path. (Page 15)
· Insert a filter in the U-FC filter cassette, attach it on the filter mount
and engage it in the light path by sliding the filter lever. (Page 15)
14
BX53
Using Built-In Filters (Figs. 14 & 15)
Use the filter knobs to engage or disengage the corresponding filters into
or from the light path.
Filter Type/Purpose
1
2
3
Fig. 14
IN
OUT
OUT
IN
1
ND6 (Neutral Density filter for light adjustment, transmittance 6%)
2
ND25 (Neutral Density filter for light adjustment, transmittance
25%)
3
LBD (for color balancing, daylight filter)
Assuming that you face the microscope from the front panel, push in a
filter knob on the right or pull out a filter knob on the left to engage the
corresponding filter in the light path. The filter can be disengaged from
the light path by reversing the above.
} When pushing in a filter knob, push it all the way to ensure engagement
of the corresponding filter. If the filter is engaged incompletely, optimum
observation may be impossible.
Mounting a Single Filter (Fig. 16)
A filter with a diameter of 45 mm 5 can be placed on the filter mount
on the base. If you need to place multiple filters, please purchase the
U-FC filter cassette.
Fig. 15
CAUTION
Even when a filter cassette is used, a filter with thickness of no
more than 3 mm can still be placed on the filter mount.
Using the Filter Cassette (Figs. 17 to 20)
Loading Filters into Filter Cassette
5
} The filter cassette accommodates filters with a diameter of 45 mm and
thickness of 2.7 mm or less.
} The filter cassette has two filter levels on the right side and one on the
left side.
} When the filter cassette is used, the ECO mode cannot be used because
the human sensor is hidden by the filter cassette.
1. Move all filter levers to the OUT position except for the one belonging to
the slot into which the filter is to be inserted.
2. Slide the lever 6 to the IN position. Make sure that it clicks securely into
place. (Fig. 17)
3. Holding the lever in the position shown in Fig. 17, put the filter into the
cassette by inserting it in the direction indicated by the arrow.
4. Insert the other two filters in the same manner.
Fig. 16
6
Fig. 17
15
Mounting the Filter Cassette
8
7
9
a
1. Fully loosen the filter cassette clamping screw †. (Fig. 18)
2. Align the key ‡ on the bottom surface of the filter cassette with the positioning slot 9 on the filter mount, then snap the filter cassette into place
from above.
3. Rotate the filter cassette to align its sides with the base. (Fig. 19)
4. Align the clamping screw † with the positioning hole ‰ on the filter
mount, then tighten the screw to fasten the filter cassette.
CAUTION
Fig. 18
When the filter cassette is installed, the stage may hit it when
lowered. Therefore, exercise caution when lowering the stage
with the filter cassette installed.
Fig. 19
Using the Filter Cassette (Fig. 20)
Usable Filters
b
Fig. 20
16
Applications
45-ND6, 45-ND25
Neutral density filter
45G-530, 43IF550-W45
Green
45O-560
Orange
B&W contrast filters
Up to three of the above filters can be loaded in the filter cassette. Moving
the levers b on the left and right sides of the cassette to the IN position
brings the corresponding filter into the light path.
BX53
5-2 Focusing Block
1
Focusing Controls
The focusing block consists of the controls listed in the following table,
which also describes the functions of the controls.
1
No.
2
5
4
Name
Function
1
Coarse adjustment
knob
2
Fine adjustment dial Fine adjusts the focus position.
3
Fine adjustment
knob
Fine adjusts the focus position. This knob
can be attached to the fine adjustment
dial on either the left or right side. (When
the microscope is shipped, it is attached
to the right-side dial.)
4
Pre-focusing lever
Sets the upper limit of the stage position
(to prevent contact between the specimen and objective).
5
Tension adjustment
ring
Adjusts the force required to rotate the
coarse adjustment knob.
Fig. 21
3
(Figs. 21 & 22)
Moves the focus position widely.
1
Fig. 22
2
Adjusting the Focus
(Fig. 23)
With both the coarse adjustment knob @ and fine adjustment knob 2,
rotating the knob in the direction of the arrow raises the stage (lets the
specimen approach the objective).
2
1
Fig. 23
17
3
4
3
CAUTION
2
1
4
5
Adjust the coarse adjustment knob tension using the tension
adjustment ring @.
Pre-focusing Lever
(Fig. 26)
} The pre-focusing lever ensures that the objective does not come in contact
with the specimen and simplifies focusing.
After focusing on the specimen with the coarse adjustment knob, turn this
lever @ in the direction of the arrow and lock; the upper limit on coarse
adjustment movement is set at the locked position.
After changing a specimen, approximate refocusing is easily accomplished
by rotating the coarse adjustment knob to reach the pre-focused position.
After this, all you have to do is make fine adjustments with the fine adjustment knob.
} Stage movement with the fine adjustment knob is not locked.
CAUTION
18
(Fig. 25)
The coarse adjustment knob tension is preadjusted for easy use. However,
if desired, you can change the tension using the tension adjustment ring
@. Turning the ring in the direction of the arrow increases tension, and
vice versa.
The tension is too low if the stage drops by itself of focus is quickly lost
after adjustment with the fine adjustment knob. In this case, turn the ring
in the direction of the arrow to increase tension.
Fig. 25
Fig. 26
The fine adjustment knob has been attached on the right side
at the factory.
Adjusting the Coarse Adjustment Knob Tension
CAUTION
4
(Fig. 24)
} The fine adjustment knob is designed detachable to prevent interference
with hand during manipulation of the fine adjustment knob or X-/Y-axis
knobs.
Usually attach the knob on the opposite side to the X- and Y-axis knobs.
1. Using the Allen screwdriver, loosen the clamping screw @ and remove
the fine adjustment knob 2.
2. Remove the seal from the fine adjustment knob screw hole on the other
side and attach the knob by reversing the removal procedure.
3. Attach a provided seal on the screw hole | of the removed fine adjustment knob.
The fine adjustment dial 3 can be operated with your fingertip or finger
surface at the same time as manipulating the X- or Y-axis knob.
Fig. 24
1
Replacing the Fine Adjustment Knob
When the pre-focusing lever is locked, a coarse movement stroke is reduced due to the microscope’s mechanism, causing the
stage not to be able to be lowered to the lower limit. To lower
the stage to the lower limit, release the pre-focusing lever.
BX53
5-3 Stage
1
2
Placing the Specimen
(Figs. 27 & 28)
} The dimensions of the slide glass should be 26 x 76 mm with thickness of
0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm.
} When observing very large specimens, remove the slide holder and place
the specimen directly on the stage.
} The maximum weight of an object such as a specimen to be placed on
the stage is 0.8 kg (including the slide holder).
} When a non-Olympus stage is going to be attached, make sure that its
weight does not exceed 4 kg.
1
Fig. 27
Microscopy with Double-Slide Holder (Fig. 27)
1. Turn the coarse adjustment knob @ to lower the stage.
2. Open the spring-loaded curved finger 2 on the slide holder and place
one or two specimen slides on the stage from the front.
3. After placing the sides as far as they will go, gently release the curved
finger.
Microscopy with Single-Slide Holder (Fig. 28)
The specimen side can easily be placed by sliding it into the slide holder
from the front.
Examining a whole specimen
Use one of the thin slide holders listed below that cause less interference
with the objectives*.
· U-HRD-4 · U-HLD-4
· U-HLS-4
* The applicable objectives are ones with 40X or less magnification (except
Apo series).
Fig. 28
Using an Oil Immersion Condenser
Use the optional U-SVRO (right-hand knob) or U-SVLO (left-hand knob)
slotted stage which prevents close contact between the specimen and
stage.
2
1
Using the Auxiliary Slide Holder
(Fig. 29)
Applicable revolving nosepieces: U-D7RE, U-D7RES, U-P6RE,
U-D6BDRE, U-P5BDRE
Fig. 29
} When a biological slide glass specimen is placed on the U-HLDT4/UHRDT4 two-slide holder (thick type), the slide holder may interfere with
the objective when the following conditions are combined;
· The objective in use has a short WD (Working Distance);
· The specimen being observed is the specimen placed on the rear
side of the side holder, and;
· The stage is in a rotated position.
If the slide holder contacts the objective, set the provided auxiliary slide
holder @ as shown in the figure and observe only the specimen on the
front side of the slide holder.
19
3
Adjusting the X- and Y-Axis Knob Tension
(Fig. 30)
1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose
the adjustment knobs.
2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob |
clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases it.
2
4
3
CAUTION
1
Fig. 30
If the tension is adjusted to tight, a creaking sound may be heard
during stage travel, and the stage stopping accuracy may be
imperiled.
} After long hours of use, the stage guide may be deviated and the stage
travel range may be decreased.
} However, this is not malfunction and can be corrected easily as described
below.
[Treatment]
Horizontal direction: Hold the specimen holder and move the stage guide
to the left and right so that it hits the stoppers.
Vertical direction:
Hold the upper stage and move it to the front and rear
so that it hits the stoppers.
Stage Feed Knob Rubber Caps (Optional)
} When the X- and Y-axis knobs are fitted with the rubber caps, the knobs can
be adjusted without slipping and fine adjustment is possible by holding
the knobs with a very light force. The knob rubber caps also reduce fatigue
after long hours of operation.
The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness
2 mm) knob rubbers are available.
To attach the knob rubbers:
First fit the larger knob rubber to the Y-axis (upper) knob from below it,
then fit the smaller knob rubber to the X-axis (lower) knob from below it.
20
BX53
4
1
Rotating the Stage
(Fig. 31)
1. Slightly loosen the stage clamping screw @.
2. The stage can be rotated both clockwise and counterclockwise by the
stage clamping screw.
CAUTION
} The angle of rotation varies depending on the positions of the X- and
Y-axis knobs.
Fig. 31
Angle of Rotation
5
1
Fig. 32
2
A click may be heard and felt during rotation. However, this is
due to the construction of the substage and does not indicate
a malfunction.
3
Clockwise
Counterclockwise
Right hand knobs
230°
20°
Left hand knobs
20°
230°
Adjusting the Stage Height
(Figs. 32 & 33)
} By lowering the position of the substage, the microscope will accommodate specimens with maximum height of 35 mm. This is useful when
observing metallurgical specimens and other thick objects.
1 Lower the stage to the lower limit, then remove the stage from the microscope.
2 Using the Allen screwdriver, loosen the substage bracket clamping screw
@ and remove the substage.
3. Turn the coarse adjustment knob and raise the focusing block 3 to where
the stopper screw 2 on the arm becomes visible.
4. Using the Allen screwdriver, loosen and remove the upper stopper screw
2.
5. Reattach substage bracket and stage.
Fig. 33
21
5-4 Observation Tube
2
1
Adjusting the Diopter
(Fig. 34)
1. Set the diopter adjustment rings on both sides to scale “0”.
2. Engage a high-power objective (40X or so) in the light path, look into
the right eyepiece with your right eye, and rotate the coarse and fine
adjustment knobs to bring the specimen into focus.
} Do not use an oil-immersion objective.
3. Engage a low-power objective (10X or so) in the light path, rotate the right
diopter adjustment ring to bring the specimen into focus. Do not touch
the coarse and fine adjustment knobs during the focusing.
4. Looking into the left eyepiece with your left eye, rotate the left diopter
adjustment ring to bring the specimen into focus.
} The above procedure adjusts the diopter with reference to the right eye,
but it is also possible to adjust with reference to the left eye. In this case,
read the above procedure by inverting “right” and “left.”
} If an eyepiece equipped with a diopter adjustment ring is inserted into
the side equipped with another diopter adjustment ring of the observation
tube, perform the above procedure without moving the diopter adjustment
ring of the observation tube from the “0” position.
1
Fig. 34
2
Using the Eye Shades
(Fig. 35)
When Wearing Eyeglasses
Use with the eye shades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.
Fig. 35
22
BX53
1
3
(Fig. 36)
When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece
micrometer disk can be inserted in one of them. When the eyepiece does
not have a diopter adjustment mechanism, however, it is hard to focus
on the micrometer disk if the operator has poor eyesight. Should that be
the case, adjust the focus with eyeglasses on.
Use 24 mm dia. x 1.5 mm thick micrometer disks.
Following Fig. 36, turn the built-in micrometer-mounting frame 2 counterclockwise to remove it from the eyepiece and place a micrometer
disk @.
Screw the micrometer mounting frame back into the eyepiece sleeve.
} Be careful not to touch the lens surface with your finger.
} The micrometer-mounting frame may be tight for certain micrometer disks.
In this case, turn the frame slowly but with a stronger force than usual.
2
Fig. 36
1
Using the Eyepiece Micrometer Disk
4
Selecting the Light Path of the Trinocular Tube
(Fig. 37)
Slide the light path selector knob @ to select the desired light path.
Trinocular tube
U-TR30-2
U-SWTR-3
Fig. 37
U-TR30NIR*
U-TTR-2**
Light path selector knob position
Pushed in
Middle position
Pulled out
20% for binocular eyepieces,
100% for binocu- 80% for camera
lar eyepieces
50% for binocu100% for camera
lar eyepieces,
50% for camera
50% for binocu100% for binocular eyepieces,
lar eyepieces
50% for camera
* With the infrared trinocular tube, infrared observation up to 1000 nm is
possible. For details, consult your Olympus representative.
** The light path selector knob is removable and can be attached to the
other side.
1. Put the edge of a coin in the groove on the tip of the light path selector
knob. Turn the coin clockwise to remove the knob.
2. Remove the cap from the other side.
3. Insert the end of the light selector knob into the hole from which the cap
has been removed. Put the edge of a con in the groove on the other end
of the knob and turn the coin counterclockwise to attach the knob.
4. Close the open hole with the cap.
23
1
5° to 35°
5
Adjusting the Tilt
(Figs. 38 to 41)
Adjust the height and tilt of the observation tube to obtain the most
comfortable viewing position.
Fig. 38
U-TBI-3
5° to 35°
U-TBI-3-CLI
5° to 35°
U-ETBI
0° to 25°
U-TTBI
0° to 25°
U-TTR-2
5° to 35°
U-TTLBI
0° to 27°
With the U-TBI-3 (Fig. 38)
Holding the binocular section @ with both hands, raise or lower it to the
desired position.
CAUTION
Fig. 39
Never attempt to force the binocular section past the upper or
lower stop position. Applying excessive force could destroy the
limiting mechanism.
} The U-TBI-3/U-TBI-3-CLI can be used in combination with only one
intermediate attachment.
} If you need photography using the U-TBI-3/U-TBI-3-CLI, use the U-TRU
trinocular intermediate tube.
With the U-ETBI/U-TTBI (Fig. 39)
The U-ETBI and U-TTBI are ergonomic observation tubes with normal
field, capable of the adjustments of the positioning angle (0° to 25°) as
well as front-rear positioning (by 45 mm) of the eyepieces. The U-ETBI is
the erect image model and the U-TTBI is the inverted image model, and
both models are of the same size.
With the U-TTLBI (Fig. 40)
Fig. 40
2
The U-TTLBI is a Tilting, Telescopic, Lifting binocular tube with the following
adjustment capabilities.
· Eyepiece angle adjustment (0° to 27°)
· Eyepiece up-down position adjustment (45 mm)
· Observation tube front-rear position adjustment (55 mm)
} When adjusting front-rear position of the observation tube, hold the base
of the observation tube as shown in Fig. 41.
} When storing the microscope, increase the angle of the eyepieces so as
to prevent the eyepieces from dropping.
} To adjust the up-down position of the eyepiece, turn the dial 2.
CAUTION
Fig. 41
24
The intermediate attachments that can be combined with the
U-TTBI and U-TTLBI are limited. For details, please contact
Olympus.
BX53
6
2
Using Eyepieces Incorporating a Micrometer
(Fig. 42)
} When the eyepieces in use incorporate a micrometer, the accuracy of
the left-right focusing adjustment (diopter adjustment) can be improved
further.
1. Looking into the right eyepiece with your right eye, turn the top of the
eyepiece @ so that the micrometer in the field of view looks sharpest
(Fig. 42).
2. Looking into the right eyepiece, rotate the coarse and fine adjustment knobs to bring the specimen and micrometer (cross lines, etc.) into focus.
3. Looking into the left eyepiece with your left eye, rotate the diopter adjustment ring 2 to bring the specimen into focus.
1
Fig. 42
5-5 Condenser
For how to adjust the centering of the condenser, see page 12.
1
Compatibility of Objectives and Condensers
Condenser
Objective
Magnification
Low
magnification
U-LC
Abbe
U-AC2
Achromat/aplanat
U-AAC
Applicable (FN 26.5)
by moving top lens
out of the light path*
(FN 26.5)
2X
10-60X
100X
Ultralow
magnification
U-ULC-2
Applicable
(FN 22)
1.25X
4X
Swing-out
U-SC3
Applicable
(FN 22)**
Applicable
(FN 22)
Applicable
(FN 26.5)
Applicable
(FN 26.5)
Applicable
(FN 26.5)
Engage top lens in
light path (FN 26.5)
* When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture
iris diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the
surroundings of the field of view may become dark.
} To obtain better illumination in camera recording using a 1.25X to 4X objective, use of the U-ULC-2 is recommended.
**When the U-LC is used with an objective with an NA of 1.0 or more, the NA of the lighting becomes insufficient.
} When the U-LC is used, it is required to attach the auxiliary lens (page 35).
25
5-6 Immersion Objectives
CAUTION
Be sure to use the provided Olympus Immersion oil.
1
Using Immersion Objectives
(Fig. 43)
1. Focus on the specimen with objectives in the order of lower-power to
higher-power ones.
2. Before engaging the immersion objective, place a drop of provided
immersion oil onto the specimen at the area to be observed.
3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.
CAUTION
Fig. 43
Since air bubbles in the oil will affect the image quality, make
sure that the oil is free of bubbles.
a. To check for bubbles, remove the eyepiece and fully open the field and
aperture iris diaphragms, then look at the exit pupil of the objective inside
the observation tube. (The pupil should appear round and bright.)
b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus
and refocus the oil immersion objective.
} If the condenser engraving shows a numerical aperture (NA) of 1.0 or
higher, the number applies only when oil is applied between the slide
glass and the top surface of the condenser. When oil is not present, the
NA is about 0.9.
4. After use, remove immersion oil from the objective front lens by wiping
with gauze slightly moistened with absolute alcohol.
CAUTION
Caution in use of immersion oil
If immersion oil enters your eyes or contacts with your skin, immediately take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin: Rinse with water and soap.
If the appearance of the eyes or skin is altered or pain persists,
immediately see your doctor.
5-7 Objectives with Correction Collar
} If the thickness scale setting of the objective with collection collar does
not match the cover glass thickness, the objective cannot manifest the full
performance. To prevent this, be sure to perform the following adjustment
when using an objective with collection collar.
Adjustment Procedure
1
Fig. 44
26
· If the cover glass thickness is known, set the correction collar @ to that
scale value. (Fig. 44)
· If the cover glass thickness is unknown, adjust the correction collar @
and fine adjustment knob alternately until the positioning with the highest
contrast is obtained.
} Be careful not to touch the correction collar @ when turning the revolving
nosepiece.
BX53
6 CAMERA RECORDING
} Use a trinocular observation tube such as the U-TR30-2 or U-SWTR-2 when shooting the video or digital camera picture
of the microscope images.
The trinocular tube accepts a camera adapter (certain TV adapters necessitate a camera mount adapter).
For details, refer to the instruction manuals for these accessories.
} Be sure to adjust the parfocality before using a camera adapter. Otherwise, the focusing of the camera image will not
match that of the image observed through eyepieces. For the parfocality adjustment method, refer to the instruction
manual for the camera adapter in use.
1
System Chart
TV camera/digital camera
Camera mount adapter
C-mount camera adapter
Camera adapter
Trinocular observation tube
U-TR30-2
U-SWTR-3
U-TTR-2, etc.
2
Selecting the Camera Adapter Magnification
The image pickup area is determined by the size of the image sensor used in the TV camera or digital camera and the
magnification of the camera adapter.
The following illustrations show the image pickup areas of camera adapters when the SWH10X-H eyepieces with a FN
of 26.5 are used (the FN of the WHN10X eyepieces is 22).
1-inch image sensor
2/3-inch image sensor
1/2-inch image sensor
WHN10X: FN22
SWH10X-H: FN 26.5
When a 0.5X camera adapter is used
When a 1X camera adapter is used
27
7 TROUBLESHOOTING GUIDE
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire
list, please contact your local Olympus representative for assistance.
Problem
Cause
Remedy
Page
Replace the bulb. Note that, even
when the filament looks normal, there
may be a wire disconnection in the
terminal section.
35
Plug power cord into the power outlet.
37
1. Observation/illumination System
a) Bulb does not light.
Bulb is burned out.
Power cord is unplugged.
b) Bulb is not lit.
It has been turned OFF due to the Set the main switch to OFF then ON
operation of the ECO mode.
again or press the ECO/NORMAL
switch twice.
c) Bulb operates, but field of view Aperture and field iris diaphragms are Adjust them to proper sizes.
remains dark.
not opened wide enough.
Condenser is lowered too much.
Adjust the condenser height position.
Light path selector knob is set to the Move the knob to the
position.
position.
d) Field of view is obscured or not Light path selector knob is in an interevenly illuminated.
mediate position.
Revolving nosepiece is not correctly
engaged.
or
Set the knob according to the observation method.
Make sure that the revolving nosepiece
clicks properly into place.
Condenser is not attached properly.
Re-attach it.
Revolving nosepiece is not attached
properly.
An objective that falls outside of the
condenser’s illumination range is used.
Push the side dovetail all the way until
it is stopped.
Use a condenser to match the purpose.
Condenser is not properly centered.
Center the condenser.
Field iris diaphragm is stopped down Open the field iris diaphragm until an
too far.
optimum size.
Bulb is not mounted correctly.
Push the pins of halogen bulb all the
way until the stop position.
Clean thoroughly.
e) Dirt or dust is visible in the field of Dirt/dust on the eyepiece.
view.
Dirt or the to surface of the condenser.
14
12
12
23
23
–
36
36
25
12
12
35
3
Dirt/dust on the specimen.
f ) Visibility is poor.
· Image is not poor.
· Contrast is poor.
· Details are indistinct.
· Image glares.
A non-UIS2/UIS objective is used.
Use only UIS2/UIS series objectives
with this microscope.
33
Condenser is lowered too far.
Adjust the condenser height position.
12
Aperture iris diaphragm is stopped
down too far.
Revolving nosepiece is not mounted
properly.
Correction collar on correction collar
equipped objective is not properly
adjusted.
Open the aperture iris diaphragm to
an optimum size.
Push the slide dovetail all the way until
it is stopped.
While focusing, turn the correction
collar to find the position with the best
contrast.
Front lens of objective is dirty.
Clean objective.
Immersion oil is not being used with Use immersion oil.
an oil immersion objective.
28
12
–
26
3
26
BX53
Problem
f ) Visibility is poor.
· Image is not poor.
· Contrast is poor.
· Details are indistinct.
· Image glares.
Cause
Immersion oil contains bubbles.
h) Image appears to waver.
Remove the bubbles.
Recommended immersion oil is not Use the Olympus-designated immerused.
sion oil.
Dirt/dust on specimen.
Dirt/dust on condenser.
g) One side of image is blurred.
Remedy
Clean it.
Page
26
26
3
Inappropriate object side or cover
glass thickness.
Objective is not correctly engaged in
light path.
Revolving nosepiece is not correctly
mounted.
Replace with glass of recommended
thickness.
Make sure that revolving nosepiece
clicks into place correctly.
Push slide dovetail all the way until it
is stopped.
Stage is not correctly mounted.
Re-attach it.
Specimen is not correctly mounted
on stage.
Revolving nosepiece is not corrected
mounted.
Objective is not correctly engaged in
light path.
Place specimen correctly on top of
stage and secure it with slide holder.
Push slide dovetail all the way until it
is stopped.
Make sure that revolving nosepiece
clicks into place correctly.
21
Condenser is not properly centered.
Center the condenser.
12
Center the condenser.
12
Adjust the condenser height position
12
Engage the LBD filter in the light
path.
10
i ) Field of view becomes only slightly Condenser is not properly centered.
brighter when the brightness adjustCondenser is lowered too far.
ment knob is rotated.
j ) Image looks reddish.
The LBD filter is not engaged in the
light path.
19
–
36
21
19
–
2. Electrical System
a) Bulb intermittently lights and goes Bulb is nearly burned out.
out.
A connector is not properly connected.
Replace bulb.
35
Check all connectors.
–
b) Bulb burns out almost immediately.
Use correct bulb type.
35
Wrong type of bulb is being used.
c) Brightness does not change when Light intensity preset button is set to Press button to OFF.
you turn light intensity adjustment ON.
knob.
d) Lamp voltage cannot be varied with Bulb is burned out.
Replace bulb.
light intensity adjustment knob.
13
35
3. Coarse/Fine Adjustment
a) Coarse adjustment knob is hard Tension adjustment ring is tightened
to turn.
excessively.
You are trying to raise stage without
coarse adjustment knob while prefocusing lever is kept locked.
b) Stage drifts down by itself or focus Tension adjustment ring is too loose.
is lost during observation.
c) Image cannot be focused.
When adjusting stage height, you forgot to reattach upper stopper screw.
d) Coarse adjustment will not go all Pre-focusing lever is locked at a too
the way up.
low height.
Loose ring.
18
Unlock pre-focusing lever.
18
Tighten ring.
Reattach upper stopper screw.
Unlock pre-focusing lever.
18
21
18
29
Problem
Cause
e) Coarse adjustment will not go all Condenser holder is too low.
the way down.
f ) Objective makes contact with speci- Specimen is mounted upside down.
men before focus is obtained.
Remedy
Raise condenser holder.
Mount specimen correctly.
Page
12
–
4. Observation Tube
a) Field of view of one eye does not Interpupillary distance is incorrect.
match that of the other.
Incorrect diopter adjustment.
Adjust interpupillary distance.
11
Adjust diopter.
22
Different eyepieces are used on left Change on eyepiece to match the
and right.
other so that both sides are the same
type.
Your view is not accustomed to micro- Upon looking into eyepieces, try
scope observation.
looking at overall field before concentrating on specimen range. You may
also find it helpful to look up and into
distance for a moment before looking
back into microscope.
–
–
5. Stage
a) Image shifts when you touch
stage.
b) Specimen stops midway on the
X-axis traverse.
c) Horizontal and/or X- and Y-axis knobs are too tight or too loose.
d) Stroke has reduced.
30
Stage is not properly mounted.
Clamp stage.
Specimen is not correctly positioned.
Place specimen correctly.
Tension of horizontal and/or X- and Y- Adjust tension.
axis knobs is too high or too low.
Stage guide is deviated.
Use treatment method described on
page 20.
21
19
20
20
BX53
8 SPECIFICATIONS
Item
Specification
1. Optical system
UIS2/UIS (Universal Infinity System) optical system (featuring infinity correction)
2. Illumination
Built-in transmitted Koehler illumination
FN (Field Number): 26.5 (super-widefield compatible)
12V, 100W long-life halogen bulb (pre-centered) 12V100WHAL-L (PHILIPS 7724)
(Average life time: Approximately 2,000 hr. when used as directed)
Light intensity voltage range: 2 V or less to 12.0 V DC (continuous)
Light intensity preset button (voltage adjustment range: 2 V or less to 12.0 V DC)
Rated voltage: 100-120/220-240 V $, 1.7/0.8 A, 50/60 Hz
Power consumption: 140 W
3. Focusing
Stage height movement by roller guide (rack & pinion)
Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse)
Full stroke range: 25 mm
Upper limit stopper
Tension adjustment on coarse focus adjustment knob
4. Revolving nosepiece
Type
U-5RE-2
U-D6RE
U-P6RE
U-D7RES
5-position revolving Universal 6-position Universal 6-position Coded 7 position
centered revolving nosepiece
nosepiece
revolving
nosepiece
nosepiece
Installable
modules
5. Observation tube
Type
Transmitted DIC prism,
transmitted light analyzer
None
U-BI30-2
U-BI-3/
U-TBI-3-CLI
U-TR30-2 U-TR30NIR
U-TTR-2
U-SWTR-3
Widefield
Widefield tilting Widefield trinocular Widefield tilting Super widebinocular tube binocular
tube
trinocular tube field trinocular
Field No.
Tube inclination
22
30°
5°-35°
continuous
Interpupillary
distance
adjustment
30°
3 steps: @ BI 100%
2 BI 20%, Camera 80%
(BI 50%, Camera 50%
for U-TR30NIR and U-TTR-2)
3 Camera 100%
None
Type
5°-35°
continuous
30°
50 mm to 76 mm
Light path
selector
6. Stage
26.5
U-SVRB-4
U-SVLB-4
Common-axis knobs on bottom right
Common-axis knobs on bottom left
Rectangular ceramic-coated, wire-driven stage
Size
Movement
mechanism
Specimen
holders (single
slide holder)
Specimen
holders (double
slide holder)
156 mm (D) x 191 mm (W)
X- and Y-axis knobs with adjustable tension
Movement range: 52 mm in vertical (Y) direction, 76 mm in horizontal (X) direction
U-HLST-4 (thick slide holder)
U-HLS-4 (thin slide holder)
U-HLDT-4 (left opening, thick slide holder)
U-HLD-4 (left opening, thin slide holder)
U-HRDT-4 (right opening, thick slide holder)
U-HRD-4 (right opening, thin slide holder)
31
Item
7. Condenser
Specification
Type
N.A.
U-LC
U-AC2
U-SC3
U-AAC
Low magnification
Abbe
Swing-out
Achromat/ Aplanat
0.75
1.10
0.9 - 0.1
1.40
Aperture iris
diaphragm
Objective
range
32
With numerical aperture scale
2X - 60X (for FN
26.5 widefield)
4X (for FN 22
widefield),
10X - 100X
(for FN 26.5 super
widefield)
1.25X (for FN 22
widefield),
2X - 100X (for FN
26.5 super widefield)
10X - 100X (for
FN 26.5 super
widefield)
8. Operating
environment
· Indoor use
· Altitude: Max. 2000 meters
· Ambient temperature: 5° to 40°C (41° to 104° F)
· Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through
70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F)
· Supply voltage fluctuations; Not to exceed ±10% of the normal voltage
· Pollution degree: 2 (in accordance with IEC60664-1)
· Installation/Overvoltage category: II (in accordance with IEC60664-1)
9. Storage environment
Temperature: Min. –25°C, Max. 65°C
Humidity: Min. 0%, Max. 95%
9 OPTICAL CHARACTERISTICS
BX53
(UIS2 Series)
UIS series objectives not listed here can also be combined with this microscope.
The following table shows the optical characteristics of combinations of eyepieces and objectives. The figure on the right
shows the performance data engraved on the objectives.
Magnification
Number of aperture
Mechanical tube
length
NOTE
Refer to the latest catalogue or consult your local Olympus
representative for the updated information on the eyepieces
and objectives that can be combined with this microscope.
Optical character
Objective
Series
PLN (PH)
Plan
Achromat
(FN 22)
PH: Phase
contrast
UPLFLN (PH)
Plan Semi
Apochromat
(FN 26.5)
UPlanFLN
(Ph)
PH: Phase
contrast
UPLSAPO
Plan
Apochromat
(FN 26.5)
UPlanSApo
PLAPON
Plan
Apochromat
(FN 26.5)
PlanApoN
MPLFLN
Plan Semi
Apochromat
(FN 26.5)
MPlanFLN
UApoN
Apochromat
(FN 22)
UApoN
Oil: Oil immersion
Iris: Iris diaphragm
Field number (FN)
Color band
W.D.
(mm)
Cover
glass
thickness
(mm)
Resolution
(µm)
Magnification
N. A .
2X
4X
10X (Ph)
20X (Ph)
40X (Ph)
50XOI
100XO (Ph)
0.06
0.1
0.25
0.4
0.65
0.5-0.9
1.25
5.8
18.5
10.6
1.2
0.6
0.2
0.15
–
–
–
0.17
0.17
0.17
–
4X
10X2 (Ph)
20X (Ph)
40X (Ph)
40XO
60X
60XOI (Ph)
100XO2 (Ph)
100XOI2
0.13
0.3
0.5
0.75
1.3
0.9
0.65-1.25
1.30
0.6-1.30
17.0
10.0
2.1
0.51
0.2
0.2
0.12
0.2
0.2
4X
10X2
20X
20XO
40X2
60XW
60XO
100XO
0.16
0.4
0.75
0.85
0.95
1.2
1.35
1.4
1.25X
2X
60XO
Cover glass thickness
Eyepiece
WHN10X (FN22)
Remark
Total mag.
Depth of
focus
(µm)
Field of
view
5.59
3.36
1.34
0.84
0.52
0.37
0.27
20X
40X
100X
200X
400X
500X
1000X
560.1
175.0
28.0
9.27
3.04
1.7
0.69
11.0
5.5
2.2
1.1
0.55
0.44
0.22
Oil immersion/Iris
Oil immersion
–
–
0.17
0.17
0.17
0.17
0.17
0.17
0.17
2.58
1.12
0.67
0.45
0.26
0.37
0.27
0.26
0.26
40X
100X
200X
400X
400X
600X
600X
1000X
1000X
127.2
22.4
7.0
2.52
1.27
1.5
0.98
0.66
0.66
5.5
2.2
1.1
0.55
0.55
0.37
0.37
0.22
0.22
Oil immersion
Correction collar
Oil immersion/Iris
Oil immersion
Oil immersion/Iris
13.0
3.1
0.6
0.17
0.18
0.28
0.15
0.13
–
0.17
0.17
–
0.17
0.17
0.17
0.17
2.10
0.84
0.45
0.39
0.35
0.28
0.25
0.24
40X
100X
200X
200X
400X
600X
600X
1000X
99.6
15.9
4.29
3.5
1.9
1.03
0.89
0.59
5.5
2.2
1.1
1.1
0.55
0.37
0.37
0.22
Oil immersion
Correction collar
Water immersion
Oil immersion
Oil immersion
0.04
0.08
1.42
5.0
6.2
0.15
–
–
0.17
8.39
4.19
0.24
12.5X
20X
600X
1326.8
398.3
0.83
17.6
11.0
0.37
Oil immersion
40X
0.75
0.63
0
0.45
400X
1.7
20XW340
0.7
0.35
0.17
0.48
200X
4.08
1.1
40XW340
1.15
0.25
0.13-0.25
0.29
400X
1.29
0.55
40XO340
1.35
0.1
0.17
0.25
400X
1.2
0.55
Marking
PlanN (Ph)
Objective type
0.55
Water immersion
Water immersion/
Correction collar
Oil immersion
33
10
ASSEMBLY
10-1 Assembly Diagram
The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly.
Assembly steps enclosed in
will be detailed on the subsequent pages.
The module numbers shown in the following diagram are merely the typical examples. For the modules with which the
module numbers are not given, please consult your Olympus representative or the catalogues.
When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
CAUTION
}Most assembly operations are possible by using the Allen screwdriver (
) provided with the microscope.
However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench (
) for clamping
the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble or
remove this module).
Observation tube
U-BI30-2
U-TTLBI
U-TR30-2, etc.
7
Eyepieces
WHN10X (FN 22)
WHN10X-H (FN 22), etc.
Intermediate attachment
U-EPA2
U-DO3
U-TRU, etc.
Observation tube
clamping screw
Revolving nosepiece
U-5RE-2
U-D6RE
U-P6RE
U-D7RES, etc.
8
UIS2 series
objective
Reflected light
mercury/xenon
lamp housing
6
9
Revolving nosepiece
clamping screw
Slide holder
clamping knobs
Slide holder
U-HLDT-4
U-HRDT-4, etc.
Vertical illuminator
BX3-URA
BX3-RFAS, BX3-RFAA, etc.
Arm
BX3-ARM
1
Power cord
Stage clamping
knob
5
Stage
U-SVRB-4
U-SVLB-4, etc.
Stage feed knob
rubber caps
U-SHG
U-SHGT
34
11
Condenser
U-LC
U-AC2
U-SC3
U-UCD8 (T2), etc.
4
100 W halogen
lamp housing
U-LH100-3
11
10
Microscope
frame
BX53F
Condenser
clamping knob
2
Lamp
adapter
clamping
screw
Auxiliary lens
for U-LC
3
100 W halogen bulb
12V100WHAL-L
BX53
10-2 Detailed Assembly Procedures
1
the Auxiliary Lens for the U-LC
2 Attaching
Condenser
} When a condenser other than the U-LC is to be used, remove the auxiliary
lens.
Attach the auxiliary lens only when the U-LC is to be used.
1. Fit the mounting tool 2 (provided with the U-LC) into the groove on the
lamp adapter @ on the rear of the microscope, and turn the tool counterclockwise to remove the lamp adapter.
2. Screw the auxiliary lens 3 (provided with the U-LC) into the tip of the lamp
adapter.
3. Attach the lamp adapter with the auxiliary lens to the original position,
and clamp the lens by fitting and turning the mounting tool clockwise.
2
3
(Fig. 45)
Fig. 45
3
Installing the Bulb
(Figs. 46 to 48)
} Use only the designated bulb 12V100WHAL-L (PHILIPS 7724).
1. Fully loosen the lamp housing clamping screw @ on top of the lamp
housing cover with the provided Allen screwdriver.
2. Lift the lamp housing cover 2 upward to remove it.
3. Turn the lamp socket 3 by 90° in the direction indicated by the arrow.
4. Holding the bulb 5 with gloves or a piece of gauze, depress the bulb
clamping lever | and insert the bulb pins 6 fully into the pin holes †
on the lamp socket. Gently release the bulb clamping lever to the original
position to secure the bulb.
1
2
Fig. 46
3
CAUTION
5. Side the lamp housing cover onto the housing base from the above.
Tighten the clamping screw @ while pressing downward on the cover.
(Fig. 46)
Fig. 47
5
CAUTION
6
7
4 7
To prevent reduced bulb life or cracking, do not touch the bulb
with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.
Caution for Bulb Replacement During Use or Right After Use
The bulb and the lamp socket are areas near these will be
extremely hot during and right after use.
Set the main switch to “
” (OFF), disconnect the power cord
from the wall outlet, then allow the old bulb and lamp housing
to cool before replacing the bulb with a new of the designated
type.
Fig. 48
35
7
Attaching the Eyepieces
(Fig. 49)
Gently insert the eyepieces all the way into the eyepiece sleeves.
CAUTION
1
2
Fig. 49
9
2
· When using the U-BI30-2 binocular tube, eyepieces with a positioning pin cannot be attached because the U-BI30-2 does
not have the positioning notch.
· When using an eyepiece with micrometer, attach it to the righthand eyepiece sleeve.
When doing so, make sure that the eyepiece positioning pin @
fits into the notch 2 on the eyepiece sleeve.
· The super-widefield trinocular observation tube is equipped with
a positioning notch on both the left and right eyepiece sleeves.
Make sure that the positioning pins on both eyepieces fits into
the respective notches.
Attaching the Revolving Nosepiece
1. Rotate the coarse adjustment knob to lower the stage.
2. Loosen the revolving nosepiece clamping screw @ slightly. Do not loosen
it too much because doing so makes it impossible to attach the revolving
nosepiece.
3. Hold the revolving nosepiece so that the mount dovetail 2 is horizontal and
insert it all the way into the dovetail groove of the microscope arm 3.
4. Tighten the revolving nosepiece clamping screw @.
} When using the U-D7RES revolving nosepiece, distribute its cable toward
the rear panel by passing it below the arm, and connect the cable to the
connector of the U-CBS control box.
} When using the U-D7REA revolving nosepiece, distribute its cable toward
the rear panel by passing it below the arm, and connect the cable to the
connector of the U-CBM control box.
} When attaching or detaching a revolving nosepiece with a cable, be sure
to disconnect the cable from the revolving nosepiece in advance.
3
1
Fig. 50
10 Attaching the Condenser
2
1
3
Fig. 51
(Fig. 50)
(Fig. 51)
1. Turn the coarse adjustment knob @ to raise the stage to its upper limit.
2. Turn the condenser height adjustment knob 2 to lower the condenser
holder to its lowest position.
3. Fully loosen the condenser clamping knob 3.
4. Position the condenser so that the aperture scale is located in front.
Gently slide the condenser along the dovetail all the way until it is stopped.
CAUTION
· When attaching a swing-out condenser with a positioning pin at
the back such as the U-SC3, attach by aligning the positioning
pin with the groove in the condenser sleeve.
· When attaching a condenser with swing-out top lens, swing out
the top lens out of the way before inserting the condenser.
5. Tighten the condenser clamping knob and gently raise the condenser
holder to its highest position.
36
BX53
1
the Lamp Housing Cable
11 Attaching
and Power Cord
2
CAUTION
10mm or
farther
3
CAUTION
4
(Figs. 52 & 53)
· Cables and cords are vulnerable when bent or twisted. Never
subject them to excessive force.
· Make sure that the main switch is set to “ ” (OFF) before connecting the power cord.
1. Connect the lamp housing cable connector @ into the connector 2 on
the rear of the microscope.
CAUTION
When installing the
microsocope, the power cord
must be apart from lamp
housing (10mm or farther)
Always use the power cord provided by Olympus. If no power
cord is provided with the microscope, please select the proper
power cord by referring to section “ PROPER SELECTION OF THE
POWER SUPPLY CORD ” at the end of this instruction manual.
2. Connect the power cord plug 3 to the AC receptacle |.
Fig. 52
5
CAUTION
6
The power cord should be connected to a grounded/earthed,
3-conductor power outlet. If the power outlet is not grounded/
earthed properly, Olympus can no longer warrant the electrical
safety performance of the equipment.
3. Plug the power cord plug 5 into the wall outlet 6.
CAUTION
Should the power cord come in contact with the hot lamp
housing, the power cord could melt and cause electric shock.
Be sure to route the power cord away from the lamp housing.
Fig. 53
37
11 LAMP HOUSING INSPECTION SHEET
{ Study the instruction manual for the lamp housing before inspection.
{ For safe use of the lamp housing, we recommend performing the following inspection periodically (every time you replace
the lamp bulb and at least every 6 months).
{ The table below identifies the check items to be observed. Put (X) if not applicable or ( ) if applicable.
{ If there is any ( ) mark noted, immediately stop use of the product, and contact Olympus for detailed inspections or replace
the lamp housing.
{ If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and
contact Olympus for detailed inspections.
{Note that the service, replacement and detailed inspections are charged after expiration of the warranty period.
If you have any questions, please contact Olympus.
Check results (Date)
Check items
1. More than 8 years have passed since original purchase or the total power ON
time has exceeded 20,000 hours.
2. Lamp does not light sometimes even though the main switch is set to on.
3. Illumination flickers when you move the lamp cable or lamp housing.
4. Lamp cable is unusually hot to the touch.
5. Scorching or burning odor is produced during use.
6. Illumination still flickers after replacement with a new lamp bulb.
7. Deformation, backlash, or looseness, etc. when you assemble the lamp housing.
(Impossibility of removing the top section of lamp housing when you attempt
to replace the lamp bulb, etc.)
8. Extreme discoloration of the lamp housing connection terminal or lamp socket.
Uneven discoloration of the left and right sections of these parts.
9. Discoloration, deformation or cracking of the lamp housing.
10. Melting, crack, deformation or solidification of the lamp cable or a wiring part.
11. Increased frequency of servicing compared to similar devices put into use at
the same time as the lamp housing.
* When the Check Result columns become insufficient, copy this sheet.
38
/
/
/
/
BX53
n PROPER SELECTION OF THE POWER SUPPLY CORD
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and
“ Certified Cord ” below:
CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the
electrical safety of the equipment.
Specifications
Voltage Rating
Current Rating
Temperature Rating
Length
Fittings Configuration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
6A minimum
60˚C minimum
3.05 m maximum
Grounding type attachment plug cap. Opposite terminates in molded-on IEC configuration appliance coupling.
Table 1 Certified Cord
A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in
Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the
agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in
your country.
Country
Agency
Certification
Mark
Country
Agency
Argentina
IRAM
Italy
IMQ
Australia
SAA
Japan
JET, JQA, TÜV,
UL-APEX / MITI
Austria
ÖVE
Netherlands
KEMA
Belgium
CEBEC
Norway
NEMKO
Canada
CSA
Spain
AEE
Denmark
DEMKO
Sweden
SEMKO
Finland
FEI
Switzerland
SEV
France
UTE
United
Kingdom
ASTA
BSI
Germany
VDE
U.S.A.
UL
Ireland
NSAI
Certification
Mark
39
Table 2 HAR Flexible Cord
APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Approval Organization
Alternative Marking Utilizing
Black-Red-Yellow Thread (Length
of color section in mm)
Black
Red
Yellow
Comite Electrotechnique Belge
(CEBEC)
CEBEC
<HAR>
10
30
10
Verband Deutscher Elektrotechniker
(VDE) e.V. Prüstelle
<VDE>
<HAR>
30
10
10
Union Technique de I´Electricite´
(UTE)
USE
<HAR>
30
10
30
Instituto Italiano del Marchio di
Qualita´ (IMQ)
IEMMEQU
<HAR>
10
30
50
British Approvals Service for Electric
Cables (BASEC)
BASEC
<HAR>
10
10
30
N.V. KEMA
KEMA-KEUR
<HAR>
10
30
30
SEMKO AB Svenska Elektriska
Materielkontrollanstalter
SEMKO
<HAR>
10
10
50
Österreichischer Verband für
Elektrotechnik (ÖVE)
<ÖVE>
<HAR>
30
10
50
Danmarks Elektriske Materialkontroll
(DEMKO)
<DEMKO>
<HAR>
30
10
30
National Standards Authority of Ireland
(NSAI)
<NSAI>
<HAR>
30
30
50
Norges Elektriske Materiellkontroll
(NEMKO)
NEMKO
<HAR>
10
10
70
Asociacion Electrotecnica Y
Electronica Espanola (AEE)
<UNED>
<HAR>
30
10
70
Hellenic Organization for
Standardization (ELOT)
ELOT
<HAR>
30
30
70
Instituto Portages da Qualidade
(IPQ)
np
<HAR>
10
10
90
Schweizerischer Elektro
Technischer Verein (SEV)
SEV
<HAR>
10
30
90
Elektriska Inspektoratet
SETI
<HAR>
10
30
90
Underwriters Laboratories Inc. (UL)
Canadian Standards Association (CSA)
40
Printed or Embossed Harmonization Marking (May be located
on jacket or insulation of internal
wiring)
SV, SVT, SJ or SJT, 3 X 18AWG
SV, SVT, SJ or SJT, 3 X 18AWG
MEMO
MEMO
Printed in Japan on September 17, 2010 M 020-06
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