Leica MZ125 User manual

Leica M Stereomicroscopes

User Manual

Dear User

Thank you for choosing our products. We hope that you will enjoy the quality and performance of Leica Microsystems products.

In developing our instruments, we have placed great emphasis on simple, self-explanatory directions. In order to utilize all the benefits of your new stereomicroscope, we suggest studying this user manual in detail. Should you have any questions, please consult your local Leica representative. You will find the address of the closest local representative as well as valuable information about products and services from Leica Microsystems on our homepage at www.leica-microsystems.com

We are gladly at your service. Customer service is a big thing with us. Not only before the sale, but afterwards as well.

Leica Microsystems (Switzerland) Ltd

Stereo & Macroscope Systems www.stereomicroscopy.com

User Manual

Your instrument is accompanied by a printed English user manual. Additional language versions and information can be found on the interactive CD-ROM. User manuals and updates are available for download on our homepage at www.stereomicroscopy.com.

This user manual contains an explanation of the safety regulations, assembly, handling and accessories of the Leica MS5,

MZ6, MZ7

5

, MZ9

5

, MZ12

5

, MZ16, MZ16 A and MZ16 FA

(if identical) stereomicroscopes. The special functions of the automated stereomicroscopes Leica MZ16 A and MZ16 FA can be found in separate user manuals.

Leica M Stereomicroscopes User Manual 3

4

Table of contents

Page

Overview

Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Controls and functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Use

Changing the magnification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Ergonomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Interpupillary distance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Eyepoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Eye contact, eyecups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Focusing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Microscope carrier: Stereoscopic and axial observation . . . . 18

Diopter settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Assembly

Overview: Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Stand

Profile column for incident light and transmitted-light stands . . 23

Transmitted-light stand, bright field, 20 W . . . . . . . . . . . . . . . . . . 23

Transmitted-light stand, bright and dark field . . . . . . . . . . . . . . . 26

Transmitted-light stand HL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Swinging-arm stand ESD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Large swinging-arm and table-clamp stands . . . . . . . . . . . . . . . 30

Optics carrier, optical accessories

Spacer rings, objective combinations . . . . . . . . . . . . . . . . . . . . . 32

Microscope carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Optics carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Binocular tubes, optical accessories . . . . . . . . . . . . . . . . . . . . . . 35

Objective nosepiece (MZ16 and MZ16 A) . . . . . . . . . . . . . . . . . . 36

Leica M Stereomicroscopes User Manual

Page

Illuminators

Transformers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Incident lamp 6 V/10 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Incident lamp 6 V/20 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Cold light sources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Fiber-optic light guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46

LED illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Supply (line) lamp 25 W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Coaxial illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Near-vertical illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Accessories

Fitting accessory tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Double-iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Drawing tube, discussion tube . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Video/phototubes:

Photography, video, TV, filming, digital imaging . . . . . . . . . . . . . 52

Attachment for vertical and oblique observation . . . . . . . . . . . . 56

Graticules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Stages, polarization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Special notes

Tips and hints: What to do if . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Care and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Calculating total magnification and field of view diameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Optical data for MS5, MZ6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Optical data for MZ7 5 , MZ9 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Optical data for MZ12 5 , MZ16, MZ16 A . . . . . . . . . . . . . . . . . . . . 66

Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Technical data electronical devices . . . . . . . . . . . . . . . . . . . . . . .74


6

Safety concept

General directions

Permitted uses

Read the user manual and safety instructions before starting up.

The Leica MS5, MZ6, MZ7

5

, MZ9

5

, MZ12

5,

MZ16 and MZ16 A stereomicroscopes are optical instruments which use magnification for improved visualization of objects, their details and specimens from technology and natural sciences. The basic outfit, which includes a stand and a light source, can be supplemented with various modules for photography, TV, dual station viewing and other applications.

Prohibited uses

• The use of the instrument in a different manner from that described in this user manual can lead to injury, malfunction and damage.

• The Leica MS5, MZ6, MZ7

5

, MZ9

5

, MZ12

5

, MZ16 and MZ16 A stereomicroscopes must not be used for eye examinations and operations.

• Do not fit different plugs. Do not dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual.

Place of use

• The Leica MS5, MZ6, MZ7

5

, MZ9

5

, MZ12

5,

MZ16 and MZ16 A stereomicroscopes are intended mainly for indoor use.

• If the stereomicroscope is used outdoors, protect it from dirt and moisture. Lamps and electrically-operated stands from Leica may not be used outdoors.

Use in ESD protected rooms

The optics carriers MS5, MZ6, MZ7

5

, MZ9

5

, MZ12

5

, MZ16 and MZ16 A, the binocular tube, ErgoTube™, ErgoWedge™ 5°–25°, the swinging-arm stand ESD and the cold light sources Leica L2 and CLS consist of ESDdischarging material (surface resistance <10 11 ohms/squared, discharge time <2 seconds, 1,000 V to 100 V).

– A connection socket for a ∅ 4 mm grounding cable is provided on the microscope carrier.

– There are two connection sockets for ∅ 4 mm grounding cables in the base of the swinging-arm stand.

Use in Clean Rooms The Leica M series can be used in clean rooms without any problems and they can be cleaned as described on page 60. Please observe the following rules:

– Do not clean our stereomicroscopes and accessories in a different manner from that described in this user manual. Do not use unsuitable cleaning agents, chemicals and techniques. Do not clean painted surfaces and accessories with rubber parts such as the ErgoTube ®

10° – 50° with chemicals.

This can damage the surfaces. Particles or grindings can contaminate the specimen.

– If the customer cleans our instruments with chemicals without our written approval he will do it at his own risk.

– In most cases we can offer special solutions on request. Some products can be modified or we could recommend other accessories for use in clean rooms.

Leica M Stereomicroscopes – Safety concept

Safety regulations

Servicing Repairs may only be carried out by Leica-trained service technicians.

Only original Leica spare parts may be used.

Responsibilities of person in charge of instrument

• Ensure that personnel who use the instrument have read and understood this user manual and in particular the safety instructions.

• Ensure that the Leica MS5, MZ6, MZ7

5

, MZ9

5

, MZ12

5

, MZ16 and MZ16 A stereomicroscopes are operated, maintained and repaired only by authorized and trained personnel.

Workplaces with stereomicroscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Dependent upon the duration of uninterrupted activity, this may lead to asthenic trouble and muscular-skeletal pain so that suitable measures must be taken to reduce the strain:

– perfect design of workplace (see p.14), work content and work process (frequently changing activities).

– detailed introduction of the personnel while considering ergonomic and organizational work-related aspects.

The ergonomic optics concept and the design of the Leica M series aim at limiting the strain on the user to the lowest possible level.

Direct contact with eyepieces can be a potential transmission path for bacterial and viral infections of the eye. Users should be made aware of the potential risk of infection. The risk can be kept at a minimum by using individual eyepieces or attachable eyecups (see p.16).

Leica M Stereomicroscopes – Safety regulations 7

8

Electrically-operated items

The safety regulations listed below apply to the following items:

– Transmitted-light stand, bright field, 20 W (page 24)

– Supply (line) lamp 25 W (page 47)

– Regulating transformer 0–7 V/40 W (page 38)

– Step transformer 4/5/6 V (page 39)

– Motor focus (separate instructions)

– Motorized Leica MZ16 A

Liquids Be careful when handling liquids.

If spilt on the equipment, they can:

– cause the stereomicroscopes and accessories to become electrically live, endangering personnel,

– cause damage to the equipment.

Power cables Inspect cables regularly for damage. Defective cables can:

– directly endanger personnel,

– cause the stereomicroscopes and accessories to become electrically live, endangering personnel.

Cable routing Route cables with care. Make sure that personnel do not get entangled with them. The instrument could fall over, fall down, become damaged, damage other equipment, or cause personal injury.

Opening the instrument Only authorized Leica personnel are permitted to repair electrical equipment. Before opening up the equipment, pull out the power cable. If you touch the live interior of the equipment, you may receive an electric shock.

Supply voltage Ensure that the voltage setting on the equipment is correct.

A wrong setting can cause damage to the equipment.

Connections When connecting power-consuming devices to the transformers, do not exceed the maximum permitted loading, as this can cause damage to the equipment.

Changing bulbs • Before changing a bulb, disconnect the power cable.

• Never attempt to touch the inside of a lamp housing or transmitted-light stand while the instrument is connected to the power supply.

• Before changing a bulb, wait until it is cool, otherwise you may burn your fingers.

Leica M Stereomicroscopes – Safety regulations

Integration in third-party products

The following must be observed if Leica products are build into third-party products:

The manufacturer of the complete system or the person putting it on the market is responsible for adhering to applicable safety regulations, laws and guidelines.

Legal requirements Adhere to general and local regulations relating to accident prevention and environmental protection.

Disposal The products described here must be disposed off in accordance with applicable local laws and regulations.

Conformity with

European Community directive

The electrically-operated accessories for the Leica MS5, MZ6,

MZ7

5

, MZ9

5

, MZ12

5

, MZ16 and MZ16 A stereomicroscopes are constructed in accordance with the latest technologies and provided with a statement of conformity with EC requirements.

Symbols used in this user manual

Safety instructions

This symbol denotes information which must be read and obeyed. Non-observance

– can put personnel at risk!

– can cause malfunctioning or damage to the equipment.

Warning about accessible hot places, e.g. bulbs.

Failure to respect it can cause burns!

Important information

This symbol denotes additional information or explanations which promote understanding.

Action

왘This symbol within the text indicates that certain operations must be carried out.

Explanatory notes

• This symbol within the text stands for additional information and explanations.

Leica M Stereomicroscopes – Safety regulations 9

8

9

7

5

4

6

Overview

Stereomicroscope

1 Magnification changer

2 Engaging ratchet positions

3a Focusing drive

3b Coarse and fine focusing

Larger knob: Coarse focusing,

Smaller knob: Fine focusing.

3c Adjustable ring

Changes the ease of movement of the coarse/fine drive.

4 Clamping screw

Holds the optics carrier in the microscope carrier.

5 Clamping screw

Holds the binocular tube (or accessory) to the optics carrier.

6 Interchangeable objective

Achromatic, planachromatic or planapochromatic

Leica MS5 Leica MZ6

Binocular tube

7 Adjustable eyepiece tubes

Interpupillary distance adjustable from

52 mm – 76 mm.

8 Wide-field eyepieces for persons wearing glasses

Diopter setting adjustable within the range from +5 to –5, and eyecups adjustable.

9 Clamping screws

These hold the eyepieces in the binocular tube.

10 ErgoTube™

The viewing angle is adjustable within the range 10° – 50°.

11 Built-in double-iris diaphragm

(MZ16/MZ16 A only)

• The special functions of the Leica MZ16 A are shown in the user manual M2-116-0.

1

3a

4

6

8

9

7

5

1

2

3a

10 Leica M Stereomicroscopes – Overview

Leica MZ7

5

8

7

5

4

6

Leica MZ9

5

8

2

1

3c

3b

4

6

7

5

7

10

5

Leica design by Ernest Igl/Christophe Apothéloz

Leica MZ12

5

8

4

6

1

2

3c

3b

Leica MZ16

8

11

4

6

10

5

9

7

Leica M Stereomicroscopes – Overview

2

1

3b

3c

1

2

3c

3b

11

Use

Magnification changer

• Zoom means continuous change of magnification.

Ratchets can be engaged for certain magnifications

(see below).

• For zooming with MZ16 A, see the user manual M2-116-0.

MS5: 5 steps

MZ6: 6:1 zoom

MZ7

5

: 7.9:1 zoom

MZ9

5

: 9.5:1 zoom

MZ12

5

: 12.5:1 zoom

MZ16 and MZ16 A: 16:1 zoom

Changing the magnification

• The magnification changer can be operated from either the right or the left side.

왘Look into the eyepieces.

왘Focus on the object (page 16).

왘Turn the magnification changer to the desired position.

Ratchet steps MZ6: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2

MZ7

5

: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4

MZ9

5

: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5

MZ12

5

: 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8

MZ16: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8, 10

• Engage the ratchets whenever magnifications need to be exactly reproducible.

왘For MZ6, MZ9

5

, MZ12

5 and

MZ16: Press the switch.

왘For MZ7

5

: Turn the knurled ring counterclockwise.

For setting ratchets at the MZ16 A, see the user manual M2-116-0.

12 Leica M Stereomicroscopes – Operation

Scale of magnifications

MS5, MZ6: Display of magnification factors from 0.63–4.

MZ7

5

: Display of magnification factors from 0.63–5.

Corresponding scales for other objective and eyepiece combinations can be affixed to the rotary knobs (page 34).

MZ9

5


MZ12

5


MZ16, MZ16 A: Display of magnification factors from 0.71–11.5

MZ16 A: For the digital display of the magnification see the user manual M2-116-0.

Magnifications and fields of view

The tables on pages 62 – 66 provide information about the magnifications and field of view diameters as a function of the magnification changer position of the eyepiece-objective combination used.

Magnification with

MZ12

5

/MZ16 planachromatic and planapochromatic objectives

When the MZ12

5

/MZ16 planachromatic and planapochromatic objectives are used on the Leica MS5, MZ6, MZ7

5 and MZ9

5

, the magnification is increased by the factor 1.25

× . This factor is taken into account in the tables.

Magnification on

MZ12

5

/MZ16

1 × plan/planapo

1.6

× planapo

0.63

× planapo

0.5

× plan

0.8

× plan

2 × planapo

Magnification on

MS5/MZ6/MZ7

5

/MZ9

5

1.25

×

2 ×

0.8

×

0.63

×

1 ×

2.5

×

Leica M Stereomicroscopes – Operation 13

Preconditions for working comfortably

Ergonomics, viewing height

• Pay great attention when setting up your stereomicroscope.

You will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given.

• For fatigue-free work, use the ErgoModules™.

• Arrange your workplace as well as possible, and pay attention to the heights of chair and table.

• When sitting, use the entire seat area and also the back support of the chair.

• Support your forearms.

• During work breaks, stand up and move around for exercise.

Please also observe the note on p. 7.

Ergonomics

Ergo objective

The reasons why users often cannot work comfortably with the 45° tube are the differing heights of the outfits, the various accessories and working distances, and the fact that the same instrument is often used by several people of differing builds. This problem can be effectively solved by using the

Leica ErgoModules™.

If you have problems with the viewing configuration on your stereomicroscope, ask your Leica consultant for the most ergonomic Leica solutions.

With the achromatic Ergo objective 0.4

×– 0.63× for the Leica

MS5, MZ6, MZ7

5 and MZ9

5 it is possible to focus in the region of 90mm (63.5 mm – 153.5 mm) ergonomically and precisely, without changing the viewing height. At the same time, magnification and working distance can be changed without any time-consuming changing of the objectives.

Viewing height, viewing angle

The viewing height is correct when your head and back are comfortably positioned.

• On the ErgoTube™, the viewing angle is adjustable from 10° – 50°.

왘Holding the eyepiece tubes at their bases, swing them up or down (see arrow).


ErgoModule™ – ErgoWedge™ 5° – 25°

– ErgoWedge™ ±15°

– ErgoModule™ 50 mm

– The ErgoTube™ 45° has eyepiece tubes which are 65 mm longer than the standard ones.

Magnification factor 1.6

×.

MZ16 A: The magnification factor can be toggled on and off using the OPT key.

Ergo objective

– ErgoModule™ 30 – 120 mm

Relubrication may be required after several years (please send the item to Leica Service).

The achromatic Ergo objective 0.4

×–0.63× can be used to ergonomically and accurately focus in the range of 90 mm

(63.5–153.5 mm) without changing the viewing height. At the same time, magnification and working distance can be changed without time-consuming change of objectives.

Motor focus Frequently repeated fine-motor tasks, such as focusing by hand, can lead to muscle fatigue and hand problems. Using the

Leica motor focusing system, every equipment can be moved up and down without applying force.

Interpupillary distance

Interpupillary distance, eye contact

The interpupillary distance is correct when you see a single circular field when viewing with both eyes.

왘Look into the eyepieces and use both hands to move the eyepiece tubes closer together or further apart.

Locating the exit pupil The distance between eye and eyepiece measures approx.

22 mm for 10 ×/21B wide-field eyepieces for persons wearing glasses and 12 mm for 10 ×/21 wide-field eyepieces.

왘Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting.


Selecting the degree of eye contact

You do not wear glasses and prefer close contact with 10 ×/21B wide-field eyepieces for persons wearing glasses:

왘Holding the diopter ring, turn the eyecup counterclockwise until it is released.

16

왘Pull the eyecup upwards.

왘Holding the diopter ring, turn the eyecup clockwise.

Persons wearing glasses need the whole of the available distance.

Move the eyecups to the lowest position.

Soft eyecups

The 10 ×/21B wide-field eyepieces for persons wearing glasses are supplied with soft eyecups, which can be attached to the integrated eyecups made of hard plastic to achieve the following:

– Prevent eye infections if several users are working at the same instrument (see p. 7).

– Protect your glasses from being scratched.

The 10 ×/21 wide-field eyepieces are supplied with laterally tapered soft eyecups.

Setting the working distance, focusing = obtaining a sharp image

• To focus, use the focusing drive to raise or lower the stereomicroscope until the feature of interest is in focus ≅ working distance to the objective.

The working distances of the various objectives are listed in the tables on pages 62 – 66.

• The focusing drive can be operated from either the right or the left side.

• When focusing on a new object, always start with the lowest magnification, because the larger field of view makes the object easier to find. Afterwards, select the desired magnification.

Leica M Stereomicroscopes – Operation

왘Place the object beneath the objective.

왘Engage the lowest magnification

(page 12).

왘Look into the eyepieces.

왘Using the rotary knob, bring the object into focus.

Coarse/fine drive The coarse/fine drive supports up to

15 kg.

– Resolution with 5 kg load: 1 µm

– Resolution with 10 kg load: 2 µm

왘Focus approximately with the larger knob.

왘Fine-focus with the smaller

(concentric) knob.

Adjusting the free running of the focusing drive

– Focusing drive

MS5 – MZ9

– Focusing drive, coarse/fine

5

Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? You can adjust the movement in accordance with the weight of the outfit and with your own personal requirements:

왘Hold the drive knobs firmly with both hands and turn them in opposite directions until you obtain the required degree of resistance during focusing.

왘Hold the left or right fine drive.

왘Turn the other fine drive until the desired resistance during focusing is reached.


Microscope carrier for stereoscopic observation

Microscope carrier

• This microscope carrier permits only stereoscopic observation, using both beam paths.

• The microscope carrier can be fitted to the drive housing in either of two positions (see page 34):

• The optics carrier can be turned sideways in the microscope carrier if the user needs to work from the side:

왘Release the clamping screw.

왘Turn the optics carrier sideways as required.

왘Retighten the clamping screw.

Microscope carrier AX

The stereomicroscopes can be equipped with the microscope carrier AX for stereoscopic and axial observation instead of the standard microscope carrier (page 34).

Different models are available for MS5, MZ6, MZ7

5

, MZ9

5 and for MZ12

5

, MZ16 and MZ16 A. The achromatic objectives and the planochromatic 1 × fit in the thread in the AX microscope carrier for MS5, MZ6, MZ7

5

, MZ9

5

(p. 33).

The planochromatic and planapochromatic objectives fit in the thread in the AX microscope carrier for MZ12

5

, MZ16, MZ16 A

(p. 33). In addition, the AX microscope carrier features a clamping screw for fixing it in one of 3 positions and an aperture which can be turned manually (see below). Otherwise, the use of and switching to the left, middle and right position are identical.

• The optics carrier cannot be turned sideways in the microscope carrier AX.

Stereoscopic observation

A three-dimensional image is required for examining plastic objects and for working.

왘Shift the stereomicroscope to the central position.


Axial observation The parallax-free imaging produces more precise results in photography, measuring and polarization.

왘Shift the stereomicroscope to the left or the right position. The object is now located directly beneath either the left or the right beam path.

• When photographing or measuring, select the beam path above which the phototube or measuring graticule is located.

Aperture A rotating aperture is build into the AX microscope carrier. It prevents interfering reflections during axial observation with coaxial incident illuminator and quarter-wave plate.

• With the AX microscope carrier for MS5, MZ6, MZ7

5

, MZ9

5

, the aperture automatically turns during the moving process.

• With the AX microscope carrier for MZ12

5

, MZ16, MZ16 A, the aperture is located underneath the microscope carrier and must manually be turned as follows:

Please note:

– In the position for stereoscopic observation through both beam paths, turn the aperture counterclockwise until it stops.

Otherwise the field of view is underexposed (vignetting).

– In the position for axial observation through the right or left beam path, turn the aperture clockwise until it stops.


20

Tips for your work

Adjusting diopter settings and parfocality

Leica stereomicroscopes are parfocally matched. This means that, once you have focused on a feature in the manner described, taking account of diopter settings, you can zoom the magnification up and down without refocusing. You only need to refocus if you want to look at higher or lower planes in the object. A prerequisite is the correct setting of the diopters and the parfocality.

• The dioptric range is from +5 to –5.

A Preparations

The following adjustments must be carried out only once by each user. The procedures for adjusting the dioptric setting and the parfocality are somewhat different if an eyepiece contains a graticule. Refer to the user manuals for the MPS modular photomicrographic systems or for measuring.

왘Install the lamp.

왘Set the interpupillary distance and, if necessary, the viewing height.

왘On the video/phototube: Move the lever to position "VIS" and open the diaphragm.

왘Set the double-iris diaphragm to "OPEN."

왘Adjust the eyecups.

왘Set both eyepieces to zero.

왘Set the microscope carrier AX for stereoscopic observation.

B Focus on the test object

1. Place a flat test object under the objective.

2. Engage the lowest magnification position.

왘Use the coarse focusing drive to set the working distance (page 16).

3. Look into the eyepieces

왘Use the focusing drive, to bring the test object into focus.

Leica M Stereomicroscopes – Operation

4. Engage the highest magnification position.

5. Optimize the focus using the focusing drive.

C Adjusting dioptric settings

6. Engage the lowest magnification position.

7. Do not look into the eyepieces.

왘Rotate the eyepieces counterclockwise in the "+" direction as far as the stop

(+5 diopters).

8. Look into the eyepieces

왘 Slowly rotate each eyepiece individually in the "–" direction until each eye sees the object sharply imaged.

D Inspecting parfocality

9. Engage the highest magnification position.

10. Refocus if necessary.

11. Slowly move the magnification changer from the lowest magnification to the highest.

• The image sharpness must remain constant (parfocal). Otherwise, repeat the procedure.


Assembly

1a

2a

The components

1a Incident-light base with stage plate

1b Transmitted-light base with glass stage plate

2a Side-faced column with drive housing, coarse/fine

2b Side-faced column with drive housing, 300 mm or 500 mm

3 Microscope carrier

4 Optics carrier

5 Interchangeable objective

6 Binocular tube

7 Wide-field eyepiece for spectacle wearers, with eyecups

8 Various ErgoModules™ or accessories for video, photography, drawing, fluorescence, coaxial incident light etc.

7

6

8

2b

1b

4

5

3

22 Leica M Stereomicroscopes – Assembly

Side-faced column ➜ incident-light base

Stand

Incident-light stand

왘

왘

Remove the stage plate.

Insert 3 Allen screws into the underside of the baseplate and secure them to the bottom end of the side-faced column.

왘 Insert the stage plate.

To fit the microscope carrier and the stereomicroscope, see page 34

Side-faced column ➜ transmitted-light base

Transmitted-light stand

Pull the power cable out of the socket in the stand.

왘

왘

Remove the glass stage plate.

Carefully invert the transmittedlight base and remove 7 Allen screws from the base.

왘

왘

왘

Lift off the lower part of the base.

Reverse the upper part of the base.

Insert 3 Allen screws into the cover plate from beneath and secure them to the bottom end of the sidefaced column.

왘

왘

왘

Replace and secure the base.

Insert the glass stage plate.

Fit the microscope carrier and the stereomicroscope as described on page 34.

Technical data see page 74

Leica M Stereomicroscopes – Assembly 23

24

Transmitted-light stand, bright field, 20 W

Follow the safety instructions given on page 7.

왘 Fit the side-faced column to the transmitted-light base as described on page 23.

Fitting the bulb Never touch the bulb when it is hot.

You may sustain burns.

• Do not touch the new 6 V/20 W halogen bulb directly with your fingers, because finger marks can cause it to crack when heated.

Remove finger marks immediately with a cloth dipped in pure alcohol.

왘 Insert the slider together with the bulb socket.

왘 Hold the new bulb in a cloth and push it into position.

왘 Push the slider carefully but completely back along the rail to the stop.

Filters • The transmitted-light stand contains a KG1 filter.

• You can insert other ∅ 50 mm filters if you need them:

왘

왘

왘

Pull out the slider.

Insert the ∅ 50 mm filter.

Push the slider carefully but completely back along the rail to the stop.

Starting up The ex-factory setting of the voltage selector is 230 (for voltages between 200–240 V) and the fuse holder contains two

160 mAT fuses.

For 100–120 V power supply:

왘

왘

Set the voltage selector to 115.

Fit the enclosed two 315 mAt fuses in the fuse holder instead of the two 160 mAT fuses (see the section

왘

"Changing fuses" on the right).

Connect the power cable to the socket of the base and to the power source.

Leica M Stereomicroscopes – Assembly

Changing fuses Use:

– Two 160 mAT fuses for voltages between 200–240 V.

– Two 315 mAT fuses for voltages between 100–120 V

(see the section "Starting up" on the left).

왘

왘

왘

왘

Disconnect the power cable from the stand.

Pull out the fuse holder.

Fuse at rear: Press out downwards.

Spare fuse at front: Pull out sideways and

왘 insert into holder at rear.

Push the fuse holder back into position.

Transmitted or incident light

On the rear side of the base, there is a socket for incident lamps of up to 20 W.

• Connect an incident lamp to the transmitted-light stand, if required.

• Either transmitted light or incident light can be switched on.

왘

왘

왘

For transmitted light: Switch in position I.

For incident light: Switch in position II.

Use thumb wheel to adjust brightness.

Switching on the transformer

왘 On the rear of the stand: Use the thumb wheel to switch on the transformer and to adjust the brightness.

Disconnection from mains power supply

왘 Remove the power cable from the power socket.

Transmitted light 왘 Switch on transmitted light.

Transmitted light, bright field

Bright field is suitable for examining translucent objects featuring contrasting structures. The object is directly illuminated from below and is seen in its natural colors against a bright background.

왘 For transmitted light, bright field:

Pull lever forwards.

Inclined transmitted light

Inclined transmitted light which traverses the object obliquely will produce effects advantageous for observing small translucent objects such as foraminifera and fish eggs.

왘 Move the lever slowly towards the column of the stand until the desired effect is obtained.


Transmitted-light stand, bright and dark field

The illumination of the transmitted-light stand bright and dark field consists of a fiber-optic light guide and a light source.

Observe the safety regulations from the manufacturer of the light source.

왘 Unplug the fiber-optic light guide from the connection in the stand.

왘 Fit the side-faced column to the transmitted-light base as described on page 23.

왘 Fit the microscope carrier and stereomicroscope as described on page 34.

왘 Attach the fiber-optic light guide at the stand and the light source.

Starting up Before using the light source, read the corresponding user manual.

Transmitted light, bright field

Bright field is suitable for examining translucent objects featuring contrasting structures. The object is directly illuminated from below and is seen in its natural colors against a bright background.

왘 Slide handle to the right until the stop: bright field.

Transmitted light, dark field

Dark field offers more information for objects poor in contrast with rather poorly marked or very fine structures. In this case, the light beams penetrate the object at a flat angle. Fine detail structures and contours contrast brilliantly and brightly from dark background.

왘 Slide the handle out to the right: dark field.


Column ➜ Base

Transmitted-light stand HL

The column seat contains Allen screws.

왘

왘

왘

왘

Remove the glass stage plate.

Invert the transmitted-light base and place it on a table.

Hold column against the column seat from underneath.

Insert the Allen screws into the column from the top and

왘 tighten them.

Invert the transmitted-light base and insert the glass stage plate.

왘 Fit additional components such as the microscope carrier and optics carrier (page 34).

Illumination Add a very luminous cold light source and a light guide with a channel to the transmitted-light stand (TVC, active ∅ 9 mm).

왘

왘

Insert light guide into the base at the rear.

Connect light guide to the light source.

Accessory condenser

➜ Basis

For stereomicroscopes with high apertures such as Leica

MZ12

5

, MZ16 and MZ16 A with objective 1.6

×, the resolution can be increased by inserting an accessory condenser.

왘 Push the accessory condenser into the lens holder at the underside of the transmitted-light base and secure it with

2 Allen screws.

Use 왘 Turn on the light source according to the manufacturer’s user manual and adjust the light intensity.

왘 Place a transparent object on the glass stage plate and focus.

Tilted mirror The built-in mirror features a clear and an obscured side and can be rotated and moved. The obscured side provides a diffused illumination. The angle of incidence in the specimen plane changes depending on the position of the mirror so that the transmitted light bright field via oblique illumination can also generate an illumination similar to dark field.

왘 Rotate and move the mirror with the black rotary knob on the right side.

왘 Adjust exact bright field and maximum illumination: move mirror to the stand column until it stops.

Rotate the mirror until it engages in the 45° position.

Transmitted-light stand

HL-RC™

Detailed information about setup, operation and use can be found in the separate instructions for use M2-216-2.


Assembly of column ➜ base

Swinging-arm stand ESD

The swinging-arm stand ESD is of conducting material (surface resistance <10 11 Ohm/squared, discharge time <2 seconds from

1,000 V to 100 V). Two sockets for connecting ∅ 4 mm ground cables are provided on the base.

왘 Insert 4 Allen screws into the base from beneath and secure them to the end of the column.

Safety ring ➜ column The safety ring secures the instrument and must always be fixed beneath the cross-member.

왘 Slide the safety ring down the column.

왘 Retighten the clamping screw.

Cross-member ➜ column

왘 Lower the cross-member onto the safety ring.


Horizontal arm ➜ cross member

왘 Slide the horizontal arm into the cross-member with the connecting piece towards you.


Drive housing ➜ horizontal arm

• The drive housing / microscope carrier can be fitted to the horizontal arm in either of two positions (see page 34).

• The circular port in the microscope carrier accepts an illuminator.

왘 Insert the peg of the inclinable focusing drive into the connecting piece.


Optics carrier ➜ microscope carrier

왘 Fit the optics carrier in the microscope carrier.

왘 Turn the optics carrier sideways in the microscope carrier as required.



Focusing The focusing knobs are located on the inclinable drive housing.

Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? You can adjust the movement in accordance with the weight of the outfit and with your own personal requirements:

왘 Hold the drive knobs firmly with both hands and turn them in opposite directions until you obtain the required degree of resistance during focusing.

Tilting

Possible adjustments

왘

왘

왘

Release the clamping screw.

Tilt the instrument.

Retighten the clamping screw.

Altering the working distance

왘

왘


Raise or lower the cross-member as required together with the horizontal arm.


Reposition the safety ring (see page 28).

Sideways movement 왘

왘


Move the instrument sideways

왘 on the cross-member.


Altering the overhang 왘

왘

왘


Displace the horizontal arm.



Table-clamp stand

Swinging-arm stand

Large swinging-arm and table-clamp stands

왘 Use the clamp to secure the column to a table top 20 mm – 50 mm thick.

왘 Insert 4 Allen screws into the base from beneath and secure them to the end of the column.

Safety ring ➜ column

Safety ring ➜ column

The safety ring secures the instrument and must always be positioned beneath the horizontal arm.

왘 Position the safety ring with the screw head uppermost and towards you, and slide it down the column.


왘 Lower the horizontal arm onto the safety ring, with its end pointing towards you.


30

Limitation The screw head must engage the notch. It limits the lateral movement. It may not be removed, and neither may the safety ring be milled down.

If the horizontal arm is swung out beyond the limit, the instrument may fall over.

Drive housing ➜ horizontal arm

This section describes the assembly of the focusing drive with side-faced carrier rod. To fit the focusing drive with peg, refer to page 28.

왘

왘

왘

Release the clamping lever.

Tilt the carrier rod downwards.

Retighten the clamping lever.

왘 Slide the drive housing with side-faced carrier rod onto the horizontal arm.

왘 Retighten the clamping lever.

The clamping levers can be positioned as required.

왘 Pull out the clamping lever along its axis and turn it.


Microscope carrier ➜ drive housing

왘 Secure as described on page 34.


왘 Secure as described on page 34.

Tilting

Possible adjustments

왘

왘

왘

Release the clamping lever.

Tilt the instrument.

Retighten the clamping lever.

Focusing with coarse/fine drive

왘 Set large distances with the larger knob.

왘 Fine-focus with the smaller (concentric) knob.

왘 Using the ring on the right-hand drive knob, adjust the ease of movement of the drive.

Altering the working distance

Sideways movement

Altering the overhang

왘

왘


Raise or lower the horizontal arm.


Reposition the safety ring (see page 30).

왘

왘


Pull out or push in the horizontal arm.


During the sideways movement, observe the limit stops (see page 30).

왘

왘

왘


Displace the horizontal arm.



Spacer rings

Optics carrier, optical accessories

Spacer rings are required in order to attach the objectives for certain instrument configurations.

• The Leica MZ9 5 is supplied with a spacer ring (10 446 393) for the smaller-diameter achromatic objectives and 1 × planachromatic objective. When the spacer ring has been removed by applying the tool provided, the larger-diameter planachromatic and planapochromatic objectives for the

MZ12 5 /MZ16 can be used.

• The Leica MS5, MZ6 and MZ7 5 can also be equipped with the larger-diameter planachromatic and planapochromatic objectives for the MZ12 5 /MZ16 if a spacer ring is fitted (10 446 172).

• When the MZ12 5 /MZ16 planachromatic and planapochromatic objectives are used on the Leica MS5, MZ6, MZ7 5 and MZ9 5 , the magnification is increased by the factor 1.25

×.

10 446 272 MZ9

5

10 446 370 MZ12

5

10 447 102 MZ16

10 447 103 MZ16 A

10 447 107

10 445 613 MS5

10 445 614 MZ6

10 446 371 MZ7

5

10 411 589 1x

10 422 562 1.5x

10 447 081 2x

10 473 832 0.8x

10 445 201 0.63x

10 422 563 0.5x

10 422 564 0.32x

Achromat

10 446 275 1x

Plan

10 446 172

10 447 148

0.4-0.63x

Ergo

10 446 157 0.5x Plan

10 447 075 0.8x Plan

10 447 157 1x Planapo

10 447 101 2x Planapo

10 447 050 1.6x Planapo

10 447 051 0.63x Planapo

Plan/Planapo

10 411 589 1x

10 422 562 1.5x

10 447 081 2x

10 473 832 0.8x

10 445 201 0.63x

10 422 563 0.5x

10 422 564 0.32x

Achromat

10 446 275 1x

Plan

10 315 306

10 445 819 1x Plan

10 446 157 0.5x Plan

10 447 075 0.8x Plan

10 447 157 1x Planapo

10 447 101 2x Planapo

10 447 050 1.6x Planapo

10 447 051 0.63x Planapo

Plan/Planapo

10 367 929

10 445 352

10 367 929

10 315 306

10 445 352


Objective combinations

Part no.

Achromat

Achromat 1

×

Achromat 1.5

×

Achromat 2

×

Achromat 0.8

×

Achromat 0.63

×

Achromat 0.5

×

Achromat 0.32

×

Ergo objective 0.4

× – 0.63×

10 411 589

10 422 562

10 447 081

10 473 832

10 445 201

10 422 563

10 422 564

10 447 148

Planachromatic

Planachromatic 1

×

Planachromatic 1

× MZ12

5 /MZ16

Planachromatic 0.5

× MZ12

5

/MZ16

Planachromatic 0.8

×

10 446 275

10 445 819

10 446 157

10 447 075

MS5

C

C

C

C

C

C

C

C

C

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

MZ6

C

C

C

C

C

C

C

C

C

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

MZ7

C

C

C

C

C

C

C

C

C

5

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

MZ9

C

C

C

C

C

C

C

C

C

C*M

C*M

C*M

5 MZ12

C

C

C

5 MZ16/MZ16 A

C

C

C

Planapochromatic

Planapochromatic

1

× MZ12

5 /MZ16

Planapochromatic

1.6

× MZ12

5

/MZ16

Planapochromatic

0.63

× MZ12

5 /MZ16

Planapochromatic

2

× MZ12

5 /MZ16

10 447 157

10 447 050

10 447 051

10 447 101

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

Accessories

Coaxial incident light

Microscope carrier AX

MS5, MZ6, MZ7 5 , MZ9 5

Microscope carrier AX

MZ12 5 , MZ16, MZ16 A

Near vertical illuminator

10 446 180

10 445 618

10 447 062

10 445 198

C

C

CA

C

Attachment for vertical and oblique observation

Objective turret for 2

× and 1× planapo objectives

10 445 156

10 447 107

C

(C)

C Compatible

CA Compatible if used with intermediate ring (order separately)

0 Intermediate ring is recommended

M Magnification increased by factor 1.25

× or higher

* Remove intermediate ring (10 446 393); already included with MZ9

5

.

(C) Large fields of view at low magnifications are not fully illuminated.

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

C

C

CA

C

C

(C)

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

CA (10 446 172) M

O (10 446 300)

C

C*M

C*M

C*M

C*M

O (10 446 300)

C

CA

CA 2

× (10 446 300)

CA 2

× (10 446 300)

C

CA (10 446 300)

CA (10 446 300)

(C) (C)

C

C

C

C

C

C

C

C

C

C

C

CA (10 446 300)

+ (10 446 393)

CA (10 446 300)

+ (10 446 393)

(C)

C

CA (10 446 300)

+ (10 446 393)

CA (10 446 300)

+ (10 446 393)

C


Microscope carrier ➜ drive housing

Microscope carrier

The microscope carrier can be attached to the focusing drive in

2 positions:

• For short working distances and for flat objects, use Position 1.

Use this position also in conjunction with the attachment for vertical and oblique observation (see detailed instructions).

• Use Position 2 for long working distances and for bulky objects.

왘 Use an Allen screw to secure the microscope carrier in the position required.

A connection socket for a ∅ 4 mm grounding cable is provided on the microscope carrier.

Microscope carrier AX • Different models are available for MS5,

MZ6, MZ7

5

, MZ9

5 and for MZ12

5

, MZ16 and MZ16 A (see p. 18).

• The microscope carrier AX can only be fitted in position 1 to the focusing drive.


Optics carrier

왘 Gently lower the optics carrier into the microscope carrier.

왘 Use the screw to secure the optics carrier in the position required.

Apply the adhesive scale of magnifications

Only for MZ7

5

:

왘

왘

왘

왘

Remove the screws.

Remove the rotary knobs.

Apply the adhesive scales.

Replace the rotary knobs.


Binocular tube

왘 Release the clamping screw.

• If you have an accessory such as an ergo module (page 14), a coaxial illuminator (page 47) or a video/phototube

(page 52), fit it now to the optics carrier.

Tighten the clamping screw.

왘 Tilt the binocular tube and insert the edge of its dovetail ring beneath the two lugs on the optics carrier.

왘 Move the binocular tube from side to side until the locating screw on the optics carrier engages the guide.


Eyepiece

Optical accessories

왘

왘

Insert a graticule, if necessary (page 56).

Release the clamping screws on the eyepieces.

왘 Insert the eyepieces fully into the eyepiece tubes. Check that they have seated correctly.

왘 Tighten the clamping screw.

• You can extend the overall magnification range by using 10

×, 16×, 25× and 40× wide-eyepieces for persons wearing glasses.

Objective Hold the objectives during assembly and disassembly to prevent them from falling on the stage plate. This applies particularly to the 2 × planapochromatic objective, which is very heavy.

Remove the specimens from the stage plate.

왘 Screw on the selected objective counterclockwise.

• Some instrument configurations require spacer rings in order to secure the objectives (page 32). These rings are fitted and removed using the tool provided.

• The Leica MZ9

5 is supplied with a spacer ring for the achromatic objectives and for the 1 × planachromatic objective.

When the spacer ring has been removed by applying the tool provided, the larger-diameter planachromatic and planapochromatic objectives for the MZ12

5

/MZ16 can be used.


36

Objective nosepiece

• Suggested for Leica MZ16 and MZ16 A

Please note: When using the objective nosepiece together with MS5, MZ6,

MZ7

5

, MZ9

5 or MZ12

5 large fields of view at low magnifications are not fully illuminated.

– Hold objectives during assembly and disassembly to prevent them from falling on the stage plate. This applies particularly to the 2 × planapochromatic objective, which is very heavy. Remove the specimens from the stage plate.

– The adjustable ring nut with the 1 × planapochromatic objective is fastened with 2 Allen screws after the adjustment. Loosen the Allen screws if you need to remove the objective.

– Attach the limit stop to the stand column. It prevents the 2

× objective from hitting the limit stop when the objective nosepiece is rotating, if the working distance was changed ×while working with the 1 objective.

– Caution: Carefully rotate the objective nosepiece and take care not to pinch your fingers between column and objective or objective nosepiece.

MZ16 A: When changing to objective 1 × or 2×, press the OPT + SEL function keys simultaneously. The display shows the current total magnification, and in REF mode it shows the current measurement with objective 1 × or 2×.

A 1 or 2 in front of the value indicates the selected objective.

Preparation Remove the securing strap from the objective nosepiece:

왘 Remove both screws (at the bottom).

Leica MZ16 and MZ16 A are delivered with a threaded adapter for objectives.

Remove the adapter to use the objective nosepiece.

왘

왘

Invert the optics carrier.

Loosen 3 screws and remove the adapter.

Assembling

1

× planapochromatic

The objective nosepiece features two threads:

– a fixed thread for the 2

× planapochromatic objective

– a thread with 2 Allen screws for the 1 × planapochromatic objective with adjustable ring nut. The adjustable ring nut is supplied separately and used to adjust the parfocality between both objectives.

Invert the objective nosepiece to simplify the assembly of the objectives.

왘 Screw on the adjustable ring nut on the thread of the 1 × planapochromatic

왘 objective until the limit stop and tighten it with the enclosed wrench.

Screw in the 1 × planapochromatic objective with the ring nut in clockwise direction on the side with the 2 Allen screws.

• The fine-pitch thread on the ring nut consists of 24 threads.

You should expect at least 40 turns.

The adjustable ring nut must never be set higher than the top surface of the nosepiece plate to prevent the nosepiece from locking while rotating.


Assembling

2 × planapochromatic

왘 Carefully turn and tighten the 2

× planapochromatic objective clockwise to the limit stop without applying excessive force.

Assembling objective nosepiece

왘

왘

왘

왘 Raise the focusing drive with the drive heads to provide sufficient space for the 2 × planapochromatic objective.

왘 Fit the objective nosepiece like a microscope carrier with the Allen screw (see page 34, position 1).

Insert the optics carrier in the objective nosepiece.


Attach the limit stop to the column of the stand so that the working distance beneath the 2 × objective will always measure approx. 15 mm.

Adjusting parfocal • Fit and adjust the 1 × and 2× planapochromatic objectives as described.

The image then remains parfocal in the range of < 0.05 mm, and refocusing after changing the objective is no longer required.

• Use a very fine, flat object (e.g. an object micrometer) for the adjustment, which allows maximum focusing even at highest resolution.

왘

왘

왘

Screw the 2 × planapochromatic objective into the beam path.

Adjusting the diopter settings and parfocality (see p. 20).

Screw the 1 × planapochromatic objective into the beam path.

왘 Observe the same object with smallest magnification without changing the settings made with the

2 × planapochromatic objective.

왘 Turn the 1

× planapochromatic objective counterclockwise until the object can be seen in focus

왘 at the smallest magnification.

Slowly zoom to the highest magnification. At the same time correct the focus by turning the

1

× planapochromatic objective.

• Verify that the object is in focus at highest magnification with the

2 × planapochromatic as well as the 1× planapochromatic objective without refocusing. Otherwise turn the objective at the ring nut until

왘 the object is focused.

Fasten the ring nut with both screws.

Do not overtighten both screws.


Illuminators

Transformers


Leica transformers may only be opened by authorized service personnel, and the power cable must be disconnected beforehand. The transformers are not to be used outdoors.

Regulating transformer

The regulating transformer 5.3 to 7.5 V/40 VA is used to operate the 6 V/10 W and 6 V/20 W lamps.

1 Connections for two lamps (maximum combined loading 40 W)

2 Connection for power cable

3 Fuse holder 2x T 1A L 250 VA

4 Power switch

I

0 on off

5 ON display: Instrument is switched on.

6 Regulating knob

7 6 V display: The standard voltage has been reached.

Technical data see page 74

38

4 5 7 6 1 1

Starting up Switching the input voltage is not necessary.

The adjustment is carried out automatically by means of the switching controller in the regulating transformer.

왘 Connect the power cable to the socket of the base and to the power source.

왘 Connect the lamp. The sockets accept two lamps with a maximum combined loading of 40 W.

Leica M Stereomicroscopes – Illuminations

2

3

Switching on and off

You can prolong the life of your bulb if you:

왘 First switch off the lamp by turning the rotary knob into the black range

왘 and only then switch the transformer on or off.

왘 Turn on the power switch.

The diode is illuminated.

왘 Use the rotary knob to adjust the brightness.

Above 6 V the diode is illuminated.

Changing fuses Fuses 1.0A, 5 ×20 (stock no. 167 651)

왘

왘

왘

Lamp off.

Power switch off.

Use a flat-bladed screwdriver to press on the slots in the fuse holders (3) and turn the fuse holders to the left.

왘

Pull out the fuse holder.

Fit a new fuse and replace the fuse holder.

In the event of overload

왘

왘

왘

왘

왘

Lamp off.

Power switch off.

Wait a few seconds.

Power switch on.

Lamp on.

Step transformer


• You can attach a 6 V/10 W lamp to the step transformer.

왘 Set the voltage selector on the rear side to 115 V or 230 V.

왘 Attach the power cable to the power supply.

왘

왘

Connect the lamp.

Adjust the brightness with the three-step switch (4/5/6 V).

Leica M Stereomicroscopes – Illuminations 39

40

6 V/10 W incident lamp

• The 6 V/10 W lamp is an inclined incident source for illuminating spatial objects

• By using two lamps, you can soften or eliminate shadows.

• At the nominal voltage (6 V), the color temperature is 2700 K.

Assembly

Lampholder ➜ clamp for side-faced columns

왘

왘

왘


Unfold the lampholder arm.


왘 Use the screw to secure the clamp to the side-faced column, either above or below the focusing drive.

왘 Use the connector to attach the lampholder arm to the clamp, on either the right or the left.

Lampholder ➜ cast foot

Lampholder ➜ objective

Lampholder ➜ inclinable focusing drive

Lamp housing ➜ lampholder arm

왘

왘

Fit the ∅ 25 mm adapter to the cast foot.

Using the connector, attach the lampholder to the adapter.

왘

왘

왘

Remove the objective.

Slide the adapter over the objective.

Using the connector, attach the lampholder to the adapter.

왘

왘

왘

왘

Unscrew a connector from the lampholder.


Using the thread, secure the lampholder to the focusing drive.


왘 Attach the lamp to the connector on the lampholder arm.

왘 Fit the filter holder, complete with KG1 heat-absorbing filter, to one of the pegs.

• A total of 4 filter holders with ∅ 50 mm filters can be attached.


Operating the lamp Observe the safety notes on page 7.

Observe the notes with respect to the use of the transformer on page 38.

왘 Connect the cable between the lamp housing and the transformer (page 38).

왘 Switch on the transformer and use the rotary knob to adjust the brightness

(page 39).

왘 Hold the lamp housing and release the clamping screw.

왘

왘

Align the light spot with the object.


왘 Swing in the heat-absorbing filter.

왘 Enlarge or reduce the size of the light spot by turning the socket of the bulb.

Changing the bulb

Disconnect the lamp cable from the transformer.

Never touch the bulb when it is hot.


• You can separate the lamp housing and the bulb socket. Line up the arrow head on the mount with its shaft on the housing.

• Do not touch the 6 V/10 W halogen bulb because finger marks can cause it to crack when heated. Remove finger marks with a cloth dipped in alcohol.

왘 Turn the socket to the separation position.

왘

왘

Pull the socket forcibly out of the housing.

Hold the new bulb in a cloth and push it into position.

왘 Replace the socket in the housing. The socket clicks into position.


42

6 V/20 W incident lamp

• The 6 V/20 W lamp is an inclined incident source for illuminating spatial objects.

• By using two lamps, you can soften or eliminate shadows.

• At the nominal voltage (6 V), the color temperature is 3200 K, ideal for photography. Set the regulating transformer to this nominal voltage (see page 38).

Assembly 왘

왘


Unfold the lampholder arm.

왘 Secure the lampholder to the lamp.

왘 Use the screw to secure the clamp to the side-faced column, either above or below the focusing drive.

왘 Using a fork wrench and the thread connector, attach the lampholder arm to the clamp, on either the right or the left.

왘

왘

Position the lamp.


• The thread connector also enables the lampholder to be connected to the cast foot.

Diffusing and other filters

• A total of 4 filter holders with ∅ 32 mm filters can be attached.

A heat-absorbing filter is built in.

왘 Push the filter holder, complete with diffusing filter, onto one of the pegs, and swing it in.


Operating the lamp Observe the safety notes on page 7.

Observe the notes with respect to the use of the transformer on page 38.

왘 Connect the cable between the lamp housing and the transformer (page 38).

왘 Switch on the transformer and use the rotary knob to adjust the brightness.

Illuminating the object 왘 Push the condenser lens forwards or backwards until the slider engages.

왘 Hold the lamp housing and release the clamping screw.

왘

왘



왘 Adjust the size of the light spot by moving the condenser lens forward or back.

왘 If necessary, swing in a filter (page 42).


44

Changing the bulb Disconnect the lamp cable from the transformer.

Never touch the bulb when it is hot.


Centering the bulb

• Do not touch the 6 V/20 W halogen bulb directly with your fingers, because finger marks can cause it to crack when heated.

Remove finger marks immediately with a cloth dipped in pure alcohol.

왘 Press the spring clips together and pull off the rear part of the housing.

왘 Hold the new bulb in a cloth and push it into position.

왘 Press the spring clips together and close the housing again.

왘 Center the bulb.

The 6 V/20 W bulb is a powerful source of illumination. The more carefully you set it up, the more uniform the lighting will be. For this reason, recenter a new bulb after fitting it.

왘

왘

왘

왘

Switch on the lamp.

Swing out the diffusing filter.

Pull the condenser lens fully out.

Hold the lamp and release the

왘 clamping screw.

Point the lamp vertically downwards at a white sheet of paper so that two

왘 filament images are seen.


왘 Using the slotted screw, bring the two filament images into focus.

왘 Displace the filament images so that they are opposite to one another, but in contact.

왘 Swing back the diffusing filter.


Leica L2 cold-light source

The antistatic Leica L2 cold-light source is powerful, small, compact, inexpensive and suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes. In addition to one or two-arm light guides, corresponding accessories are also available for the coaxial, vertical and transmitted-light illumination methods. The Leica L2 can be directly connected on the stand.

The light guides can be mounted with arms in the threads on the microscope carrier. For instructions on mounting the clamp for fiber-optic light guides, see page 46.

For detailed information, refer to the separate user manual

Leica L2 M2-288-0.

When in use, the Leica L2 fiber-optic light source must always be locked into an adapter to ensure stability.

Adapter ➜

300 mm focusing drive

왘 Secure the clamp to the 300 mm focusing drive by tightening the screw.

왘 Using the mounting rail, slide the fiberoptic light source into the adapter fork until it engages.

Adapter ➜ baseplate

When mounted on the baseplate, the light source can be used as a stand-alone unit.

왘 Using the mounting rail, slide the fiberoptic light source into the adapter fork until it engages.


46

Cold light source Leica CLS series

The Leica CLS series is a high-performance line for high light intensity within the smallest possible space and flicker-free white light with the lowest possible thermal effect on the objects. The comprehensive line of accessories enables unlimited use. The CLS series is produced of antistatic material.

When using the ring lamp (

∅ 76 mm) on the planachromatic objective 0.8

× (∅ 80 mm), a special adapter (10 447 078) is required.

For a detailed information, refer to the user manual Leica CLS.

Fiber-optic light guide

• The clamp of the fiber-optic light guide cannot be attached to the microscope carrier AX.

왘 Turn the clamping screw out of the clamp.

왘 Hold the clamp in the opening of the microscope carrier.

왘 Push the clamping screw through the holes in the microscope carrier and in the broader part of the clamp, and screw it finger-tight to the narrow part of the clamp.

왘

왘

왘

왘

Insert the fiber-optic light guide into the clamp.

Switch on the power unit.



Leica LED illumination

The Leica LED1000 (Laser Emitting Diode) illumination is available with ring illuminator and/or spot and suitable for routine tasks with the Leica MS5, MZ6, MZ7

5

, MZ9

5 stereomicroscopes.

LEDs, which do not develop any heat, are used as illuminators.

The color temperature measures 5000 K (daylight).

For details see the separate instructions for use for the LED illumination.


Supply (line) lamp 25W


왘 Fit the lamp holder and lamp as described below (incident-light

왘 lamp 6 V/10 W).

Connect the 25 W supply (line) lamp to the power supply.

왘

왘

Switch on the supply (line) lamp.

Align the light spot with the object as described.

Coaxial illuminator

• This illuminator is for observing and photographing flat, highly-reflecting objects such as wafers, integrated circuits and polished metal sections.

• The coaxial incident light housing fits like an accessory tube between the optics carrier and the binocular tube, or between the optics carrier and the phototube (page 50).

• For the MZ7

5 and MZ9

5

, fit the objective preferably to the spacer ring (10 446 300), page 32. This will completely illuminate the largest field of view.

• A quarter-wave plate is required when the microscope carrier AX is used in the vertical position.

왘 Using the clamping screw, secure the quarter-wave plate to the objective mount.

왘 Using the knurled ring, turn the quarter-wave plate until the required effect is obtained.

Please observe the notes on the use of the aperture in the

AX microscope carrier (p. 19).


Near-vertical illuminator

• The near-vertical (approx. 5°) light beam illuminates depressions and cavities.

• Shadowing by tools is eliminated.

• This illuminator can only be used with the achromatic objectives having focal lengths of 100, 150, 175 and 200 mm.

• Spacer rings are required for the Leica MZ7 5 , MZ9 5 , MZ12 5 to secure the near-vertical illuminator to the optics carrier

(page 32).

왘

왘

Unscrew the objective.

Release the clamping screw and remove the adapter ring from the incident-light housing for near-vertical light.

For MS5, MZ6: 왘 Screw the adapter ring onto the microscope carrier.

왘 Secure the incident-light housing to the adapter ring.

For Leica MZ7

5

,

MZ9

5

, MZ12

5

:

왘 First screw the spacer ring(s) (page 32) onto the microscope carrier, then screw

왘 the adapter ring to the spacer ring.

왘 Secure the incident-light housing to the adapter ring.

Screw the achromatic objective (see above) into position.

왘 Release the left or right clamping screw and change the cover plate accordingly.

48 Leica M Stereomicroscopes – Illuminations

왘 Attach the fiber-optic light guide on either the left or the right.

To switch on the cold-light illuminator, refer to the user manual provided with it.

No light?

왘 Turn the rotary knob.

• A movable prism directs the light from the left or right outlet onto the object.


Accessories

Assembly

Tubes

• All accessory tubes which fit between the optics carrier and the binocular tube are fitted in the same manner.

• In the dovetail ring of the optics carrier is a locating screw which must engage the notch in the accessory (page 35).

This is best accomplished by moving the accessory slightly from side to side.

왘

왘


Insert the accessory tube into the dovetail ring in the same manner as the binocular tube (page 35) and move it slightly from side to side until the locating screw

왘

왘 engages the notch.


Fit the binocular tube on the accessory tube in the same manner.

Double-iris diaphragm

A double-iris diaphragm is built into the MZ16 and MZ16 A. It is operated by means of a knurled ring. A separate double-iris diaphragm is available as accessory for the other instruments.

The double-iris diaphragm for MS5–MZ12

5 can be inserted between optics carrier and binocular tube or video/phototube.

Set the lever to OPEN: The double-iris diaphragm (aperture diaphragm) is open.

The depth of field increases as the lever is moved from OPEN to 1. Remember, however, that

– brightness decreases, so exposure times become longer

– resolution deteriorates.

50 Leica M Stereomicroscopes – Accessories

Drawing tube

• The drawing tube is for both left-handers and right-handers.

Fit the mirror on the left-hand or right-hand side of the optics carrier.

왘 Fit the drawing tube between optics carrier and binocular tube as described on

왘 page 50.

Place a sheet of paper beneath the mirror.

왘 Use a table lamp to illuminate the paper.

Drawing 왘

왘

Move the lever to the circle.

Look into the binocular tube, position the object, and focus on it.

왘

왘

Move the lever to the black spot.

Look into the binocular tube and balance the brightness so that the object, the drawing surface and the pencil are all seen together.

• In general, the paper needs to be illuminated strongly, but the object only weakly.

왘 Move the lens mount of the drawing tube forwards or backwards until the drawing surface is sharply imaged.

왘 Now you can draw the feature.

• If you place a graduated scale beneath the object and draw its intervals as well, you can easily determine the image scale on the paper.

Discussion tube

At the discussion tube two observers see the same erect, laterally-correct, three-dimensional image.

The assembly and use of the discussion tube are described in the separate user manual M2-263-0.

Leica M Stereomicroscopes – Accessories 51

Video/phototubes

1”, 2/3”, 1/2”, 1/3”

C-Mount, CCD cameras

Leica DC cameras

SLR camera housing

Leica

MPS30 / MPS60 photoautomats

B-Mount

3-chip 2/3”

CCD camera

B-Mount

3-chip 1/2”

CCD camera

C-Mount

1”

CCD camera

11 543 703 11 543 702 11 543 706

Adapter

SLR

11 541 006

1”, 1x

11 541 007

2/3”, 0.63x

10 445 928

1/3”, 0.32x

10 445 929

1/2”, 0.5x

11 541 016

1/2”, 0.5x

10 446 261

2/3”, 0.63x

10 446 307

0.8x

10 446 175

10 162 226

10 445 541

10 404 207

10 446 120

8x

10 445 304

10x

10 445 305

16x

10 445 932

11 541 038

0.55x -1.1x

10 445 930

1x

10 446 121 8x

10 446 131 10x

10 446 132 16x

10 445 924 50%

10 446 229 100%

HD F 10 446 308

HD V 10 446 309

Leica MZ16, MZ16 A

H

10 445 931

HD50

10 446 197

A

10 445 925

52

10 445 927

Leica M Stereomicroscopes – Accessories

Leica MS5, MZ6, MZ7

5

, MZ9

5

,

MZ12

5

, MZ FLIII

• Video/phototubes are designed to support modular photomicrography systems, and Leica digital, video, film or SLR cameras.

• The modular photomicrographic systems and the Leica digital cameras are detailed in a separate user manual, along with instructions for their assembly and use.

• When you are not taking photographs, engage the observation beam path.

• Accessories such as the coaxial incident illuminator always fit between the stereomicroscope and the phototube.

Video/phototube A

This tube does not permit stereoscopic observation. The image is studied either in the camera viewfinder, or down the focusing telescope of the camera. In the video/photo beam path, 100% light is available.

Trinocular video/phototube 50%

왘 Fit the trinocular video/phototube on the optics carrier in place of the binocular observation tube (page 35).

For examples on camera assembly see page 54.

Partition of light • 50% to both eyepieces

• 50% in the video/photo beam path

Stop Before taking the photograph, close off the right eyepiece to keep out stray light.

왘 Move lever to the left.


Trinocular video/phototube 100%

Fitting: See "Trinocular video/phototube 50%".

Switchover 왘

왘

Observation: Push lever in.

Photography: Pull out lever to the left.

Light partition setting "VIS"

• 100% in both eyepieces.

Light partition setting "PHOT"

• 100% in the left eyepiece

• 100% in the video/photo beam path.

Camera setup, variants

As shown in the setup diagram on page 52, there are various possibilities for adapting an MPS, video, digital or SLR camera.

Example:

With this equipment you can, for example, attach an MPS camera (see detailed description in the MPS user manual), an

SLR camera, or even a digital or video camera.

왘 Fit the 1 × video/photo objective.

Example:

With this equipment you can, for example, attach a digital or video camera.

왘 Fit the video objective (2) on a Leica

DC digital camera (3) with the C-mount connection.

왘 Connect the video objective with the mounted Leica DC digital camera on

왘 the right output of the video/phototube.

Tighten the coupling ring (1).

54 Leica M Stereomicroscopes – Accessories

Video/phototube HD50

왘 Use the video/phototube HD50 between the optics carrier and the binocular tube

(page 50).

Switchover 왘

왘

Observation: Push lever in.

Documentation: Pull lever out.

Light partition for "observation"

• 50% to both eyepieces

• 50% in the left-hand video/photo beam path

• 50% in the right-hand video/photo beam path

Light partition for "documentation"

• 100% to left video/photo beam path

• 50% to right eyepiece

• 50% in the right-hand video/photo beam path

Camera setup, variants As shown in the setup diagram on page 52, there are various possibilities for adapting an MPS, video, digital or SLR camera.

Example:

왘 Fit the video/photo objective

H on the left-hand side.

With this equipment you can, for example, attach an MPS camera (see detailed description in the MPS user manual), an

SLR camera, or even a digital or video camera.

Example:

With this equipment you can, for example, attach a digital or video camera.

왘 Fit the video objective (2) on a

Leica DC digital camera (3) with

왘 the C-mount connection.

Connect the video objective with the mounted Leica DC digital camera on the right output of the video/phototube.

왘 Tighten the coupling ring (1).


56

Attachment for vertical and oblique observation

• The attachment for vertical and oblique observation enables spatial objects to be observed from above, and also from all around at an angle of 45°.

• The microscope carrier must be fitted in the lower position 1 (page 34).

• For MS5, MZ6: Fit the 1 × achromatic objective by screwing its adapter ring directly into the optics carrier.

• Spacer rings are required for the Leica

MZ7

5

, MZ9

5

, MZ12

5 and MZ16 to secure the attachment for vertical and oblique observation to the optics carrier (page 32).

• Only usable with 1 × achromatic objective.

A detailed description can be found in the user manual M2-266.

Graticules

• The graticules for length measurements and numbering are fitted in mounts and can be inserted into the eyepieces.

• A special measuring graticule is available for the Leica MZ16

A (see the user manual M2-116).

• A high-precision object micrometer with 50-mm scale,

0.1 mm and 0.01 mm graduation, allows for calibration corresponding to the selected magnification.

• Preferably measure with the microscope carrier AX in vertical position. The measurements are more accurate without the convergence angle in the stereoscopic image.

• Graticules show boundary lines for all film formats.

Inserting the graticule

왘

왘

Unscrew the insert at the knurled ring.

Clamp the graticule onto the insert with a little bit of pressure. Check for correct seating!

왘

왘

왘

Tighten the insert with graticule.

Insert the eyepiece.

Look into the eyepiece: Align the graticule by turning the eyepiece in the tube and tighten the clamping screw.


Leica M Stereomicroscopes – Accessories

Stages

The ∅ 120 mm stages can be used in those incident and transmitted-light stands which have a ∅ 120 mm port.

Gliding stage The gliding stage can be moved in all directions and rotated completely.

왘

왘

Place the

∅ 120 mm gliding stage in the ∅ 120 mm port and secure it with the eccentric lever.

Insert the ∅ 120 mm glass stage plate or the black/white stage plate.

Cup stage • The cup stage can be inclined as required.

• You can secure objects to its rubberized upper surface with needles.

• The movable holder accepts petri dishes.

왘

왘

Place the ∅ 120 mm support ring in the

∅ 120 mm port.

Place the cup stage on it.

Thermo stages The Leica MATS thermocontrol system is a heating system for microscopes and stereomicroscopes. Leica MATS allows the observation of temperature-sensitive specimens and living cells in biology, medicine and pharmaceutics under exact temperature conditions.

For details see M2-227-0 instructions for use.

Polarization, transmitted light

The analyzer, in combination with either the rotatable polarization stage or the polarizer on glass stage plate, enables doublerefracting materials to be studied. These can include organic crystals, mineral grains, thin sections of rock or bone, polymers, stressed glass, and liquid crystals.



Notes

The field of view is partly shadowed.

Tips and hints: What to do if …

Remedy:

• Set the interpupillary distance correctly (page 15).

• Locate the eyepoint (page 15).

The image does not stay in focus.

Remedy:

• Fit the eyepieces correctly (page 35).

• Adjust your dioptric settings exactly as described (page 20).

The focusing drifts downwards or can be moved only with difficulty.

Remedy:

• Adjust the ease of movement (pages 16, 29, 31).

If an electrical fault develops, first check the following:

• Is the voltage selector set correctly (bright-field transmitted light stand, page 24; transformer, page 38)?

• Is the power switch on?

• Is the power cable attached correctly?

• Are all connecting cables attached correctly?

• Are the fuses in order?

The image is too dark.

Remedy:

• Turn up the regulating knob high enough (bright-field transmitted light stand, page 24; transformer, page 38).

• Open the double-iris diaphragm of the phototube.

The image on the screen is too dark.

TV, photography

Remedy:

• Adjust the video camera / monitor system in accordance with the directions of the manufacturer.

• Refer to the remedies listed above under "The image is too dark."

Blurred photographs.

Remedy:

• Focus precisely (page 16).

• Adjust your dioptric settings exactly as described (page 20).

Color photographs have a yellow cast.

Remedy:

• Use artificial-light film.

• Turn the regulating knob of the transformer clockwise to the position of maximum voltage (bright field transmitted-light stand, page 24; transformer, page 38).

58 Leica M Stereomicroscopes – Notes

In this section

Care, maintenance

We will show you how to look after your microscope and how to clean it.

We guarantee the quality.

You are working with a high-precision, high-performance instrument.

Accordingly, we guarantee the quality of each of our instruments. The guarantee covers all faults in materials and manufacture. It does not, however, cover damage resulting from careless or improper handling.

Please handle your valuable optical instrument with care. It will then fulfill its function with the same precision for decades. Our instruments are known for that!

If, contrary to expectations, your stereomicroscope develops a fault, please call in a specialist – contact your local Leica agent or Leica Microsystems (Switzerland) Ltd,

CH-9435 Heerbrugg.

Protect your instruments

• From moisture, vapors, acids, alkalis and corrosive substances, and keep chemicals away from the instruments.

• From improper handling.

Never dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual.

• From oil and grease.

Do not grease guide surfaces or mechanical parts.

Leica M Stereomicroscopes – Notes 59

Therefore:

Cleaning

Dust and dirt will affect the quality of your results.

왘 Put a dust cover over the instrument during breaks in work.

왘 Use dust caps to protect tube openings, tubes without

왘 eyepieces, and eyepieces.

Remove dust with a pneumatic rubber bulb or with a soft brush.

왘 Clean objectives and eyepieces with special optic cleaning

왘 cloths and with pure alcohol.

When accessories are not being used, protect them against dust.

Cleaning polymer components

Some components are made of polymer, or are polymer-coated.

They are, therefore, pleasant and convenient to handle. The use of unsuitable cleaning agents and techniques can damage polymers, so always observe the following rules:

Never clean polymers this way

• Never clean them in an ultrasonic bath. The polymer can become brittle and later break.

• Never use corrosive cleaning agents or ones containing acetone.

• Never use solvents for cleaning (except for ethanol and isopropanol).

You can clean polymers harmlessly

• With warm soapy water, wiping down afterwards with distilled water.

• With ethanol (industrial alcohol) and isopropanol.

When cleaning with ethanol or isopropanol, adhere to the appropriate safety regulations.

60 Leica M Stereomicroscopes – Notes

Total magnification and field of view diameter

Calculation

r

MO Magnification of objective

ME Magnification of eyepiece z q

Magnification changer position

Tube factor, e.g. 1.5

× for coaxial incident light,

45° ErgoTube TM 1.6

×

Factor 1.25

× if the planachromatic and planapochromatic objectives of the MZ12

5

/MZ16 are used on the

MS5, MZ6, MZ7

5 or MZ9

5

NFOV Field number of eyepiece. The field numbers are printed on the eyepieces:

10 × = 21, 16× = 14, 25× = 9.5, 40× = 6.

Example r

MO Eyepiece 1

×

ME z

25 ×/9.5 eyepiece q

Zoom position 4

Tube factor 1.5

× (because of coaxial illuminator).

Factor 1.25

×

Magnification down binocular tube:

MTOT VIS = MO × ME × z × q × r 1 × 25 × 4 × 1.5 × 1.25 = 187.5 ×

Diameter of field of view:

∅ OF :

NFOV

MO × z × q × r

9.5

1 × 4 × 1.5 × 1.25

= 1.3 mm

Leica M Stereomicroscopes – Notes 61

Optical data of Leica MS5, MZ6

Objectives

1 × Plan

1 × Achromat

0.8

× Plan*

1

× Planapo* 1.6

× Planapo*

2 × Achromat

0.63

× Planapo*

0.8

× Achromat

0.5

× Plan*

0.63

× Achromat

0.32

× Achromat 0.5× Achromat 1.5× Achromat

81 Plan

89 Achromat

112 Plan

55 Planapo 19 Planapo

27 Achromat

Working distance (mm)

97 Planapo

112 Achromat

135 Plan

149 Achromat

297 Achromat 187 Achromat 49 Achromat

Eyepieces

10 ×/21B

16 ×/14B

25

×/9.5B

40 ×/6B

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

25.2

32

40

50

64

80

100

128

160

16

20

25

32

40

6.3 33.3

8 26.3

7.9

10

26.6

21

10 21 12.5 16.8

12.5 16.8 15.6 13.5

13.1

10.5

8.4

6.6

5.3

20

25

31.3

40

50

10.5 32

8.4 40

6.7 50

5.3 64

4.2 80

12.6 16.7

16 13.1

20

25

5

10.5 8

8.4 10

42

6.4 32.8

26.3

21

5.3 16

4.2 20

3.3 25.6

2.6 32

13.1

10.5

8.2

6.6

3.9

5

6.6 12.8 16.4 10

12.5

15.6

20

25

53.8

42

6.3 33.3

7.8 26.9

21

16.8

13.5

10.5

8.4

2

2.5

3.1

3.9

5

6.3

7.8

10

12.5

105

84

67.7

53.8

42

33.3

26.9

21

16.8

8

10

12.5

16

20

3.2 65.6

4 52.5

9.4

11.9

22.3

17.6

5 42 14.9 14.1

6.3 33.3 18.7 11.2

26.3

21

23.9

29.9

16.8 37.3

13.1 47.8

10.5 59.7

8.8

7

5.6

4.4

3.5

10.1 22.2 12.6 17.8

12.8 17.5 16 14

16

20

14 20

11.2 25

11.2

9

25.6

32

40

51.2

64

8.8

7

32

40

5.6 50

4.4 64

3.5 80

20.2

25.6

32

40

7 51.2

5.6 64

4.5 80

3.5 102.4

2.8 128

11.1

8.8

8.1

10.2

27.7

22

6.3

8

4.4 20.5 10.9 16

3.5 25.6

2.8 32

2.2 41

1.8 51.2

8.8

7

5.5

4.4

20

25

32

40

35.6

28

7 12.8 17.5 10

5.6 16 14 12.5

22.4

17.9

3.2 70

4 56

5

6.4 35

5 44.8 8

6.3 35.6 10

14 8

11.2 10

9

7

12.5

16

5.6 20

28

22.4

17.9

14

11.2

12.8

16

20

25.6

32

44.8 15

28

22.4

17.5

14

19.1

23.9

29.9

38.2

47.8

11.2 59.7

8.8 76.4

7 95.5

14.9

11.7

9.4

7.5

5.9

4.7

3.8

2.9

2.3

15.8 15

20 11.9

19.7

25

12.1

9.5

31.5

40

25

31.3

40

50

9.5

7.6

5.9

4.8

31.3

39.1

50

62.5

7.6

6.1

4.8

3.8

50

62.5

80

100

62.5

80

100

3.8

3

2.4

78.1

100

125

3

2.4

1.9

125

160

200

7.5 12.6 18.8

5.9 16 14.8

9.8

12.5

24.2

19

4.8 20

3.8 25

3 32

2.4 40

11.9

9.5

7.4

5.9

15.6

19.5

25

31.3

15.2

12.2

9.5

4.9

6.3

7.8

9.8

12.5

48.5

37.7

30.4

24.2

19

7.9

10

12.5

15.6

20

7.6 15.6 15.2 25

30.1

23.8

19

15.2

11.9

9.5

23.5

29.9

37.3

46.6

59.7

74.6

10.1

7.9

6.4

5.1

4

3.2

1.9 50

1.5 64

1.2 80

4.8

3.7

3

39.1

50

62.5

6.1

4.8

3.8

19.5

25

31.3

12.2

9.5

7.6

31.3

40

50

7.6 93.3

5.9 119.4

4.8 149.3

2.5

2

1.6

9.5 31.5

7.5 40

6 50

4.8 62.5

3.8 80

3 100

2.4 125

1.9 160

1.5 200

7.6 50.4

6 64

4.8 80

3.8 100

3 128

2.4 160

1.9 200

1.5 256

1.2 320

4.8 20.2 11.9 15.8 15.2

3.8 25.6 9.4 20 12

3 32

2.4 40

1.9 51.2

1.5 64

7.5

6

4.7

3.8

25

31.3

40

50

9.6

7.7

6

7.9

10

12.5

15.6

20

4.8 25

30.4

24

19.2

15.4

12

9.6

12.6

16

20

25

32

40

1.2 80

0.9 102.4

0.8 128

3

2.3

62.5

80

1.9 100

3.8

3

2.4

31.3

40

50

7.7

6

4.8

50

64

80

19

15

37.6

47.8

12 59.7

9.6 74.6

7.5 95.5

6 119.4

4.8 149.3

3.8 191

3 238.8

6.4

5

4

3.2

2.5

2

1.6

1.3

1

MS5: Positions 0.63, 1, 1.6, 2.5, 4

* When using the planachromatic and planapochromatic objectives MZ12

5

, the magnification is increased by the factor 1.25

×.

62 Leica M Stereomicroscopes User Manual

Objectives

Ergo objective

0.4

×–0.63×


63.5 mm 153.5 mm

Eyepieces

10 ×/21B

16

×/14B

25 ×/9.5B

40

×/6B

4

5

52.5

41.2

6.4 32.8

8 26.3

4

5

2.6 80.8

3.3 63.6

51.2

41.2

10.2 20.6

12.7 16.5

6.6 31.8

8.2 25.6

15.9 13.2 10.3 20.4

20.4 10.3 13.2 15.9

25.5 8.2 16.5 12.7

6.4 35

8.2 27.3

10.2 22

12.7 17.6

4 54.6

5.3 42.3

6.6 33.9

8.2 27.3

16.3 13.7 10.5 21.3

20.4 11 13.2 17

25.5

32.6

40.8

8.8 16.5

6.9 21

5.5 26.3

13.6

10.6

8.5

10 23.8 6.5 36.5

12.7 18.7 8.2 29

15.9 14.9 10.3 23

19.9 11.9 12.9 18.4

25.5 9.3 16.5 14.4

31.8

39.8

7.5

6

20.6

25.7

11.5

9.2

51

63.7

4.7 32.9

3.7 41.2

7.2

5.8

16 14.9 10.4 23

20.4 11.8 13.2 18.2

25.5

31.8

9.4

7.5

16.5

20.6

14.5

11.7

40.8

51

63.7

81.5

101.9

5.9

4.7

3.8

2.9

2.4

26.3

32.9

41.2

52.7

65.8

9

7.3

5.8

4.6

3.6

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4


Optical data of Leica MZ7

5

, MZ9

5

Objectives

1 × Plan

1

× Achromat

0.8

× Plan**

1

× Planapo** 1.6

× Planapo**

2

× Achromat

0.63

× Planapo**

0.8

× Achromat

0.5

× Plan**

0.63

× Achromat

0.32

× Achromat 0.5× Achromat 1.5× Achromat

81 Plan

89 Achromat

112 Plan

55 Planapo 19 Planapo

27 Achromat


97 Planapo

112 Achromat

135 Plan

149 Achromat

297 Achromat 187 Achromat 49 Achromat

Eyepieces

10

×/21B

16 ×/14B

25 ×/9.5B

40 ×/6B

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

5

6*

6.3 33.3

8 26.3

7.9

10

26.6

21

10 21 12.5 16.8

12.5 16.8 15.6 13.5

16

20

25

32

13.1

10.5

8.4

6.6

20

25

31.3

40

10.5

12.6

16

20

25

32

8.4 40

6.7 50

5.3 64

16.7

13.1

5 42

6.4 32.8

10.5 8

8.4 10

5.3 16

4.2 20

3.3 25.6

26.3

21

13.1

10.5

8.2

3.9

5

53.8

42

6.3 33.3

7.8 26.9

6.6 12.8 16.4 10

12.5

15.6

20

21

16.8

13.5

10.5

2

2.5

3.1

3.9

5

6.3

7.8

10

105

84

67.7

53.8

42

33.3

26.9

21

3.2

4

5

8

10

12.5

16

65.6

52.5

42

6.3 33.3 18.7

26.3

21

9.4

11.9

14.9

23.9

29.9

16.8 37.3

13.1 47.8

40

50

60

5.3 50

4.2 62.5

3.5 75

4.2 80

3.4 100

2.8 120

2.6 32

2.1 40

1.8 48

6.6 25

5.3 31.3

4.4 37.5

8.4 12.5 16.8 20

6.7 15.6 13.5 25

5.6 18.8 11.2 30

10.5 59.7

8.4 74.6

7 89.6

22.3

17.6

14.1

11.2

8.8

7

5.6

4.4

3.5

2.8

2.3

2.5

3.2

4

5

6*

0.63

0.8

1

1.25

1.6

2

10.1 22.2 12.6 17.8

12.8 17.5 16 14

16

20

25.6

32

14

11.2

8.8

7

20

25

32

40

11.2

9

7

20.2

25.6

32

40

51.2

5.6 64

11.1

8.8

7

5.6

4.4

3.5

8.1

10.2

12.8

16

20.5

25.6

27.7

22

17.5

14

10.9

8.8

6.3

8

10

12.5

16

20

35.6

28

22.4

17.9

14

3.2

4

5

6.3

8

11.2 10

70

56

44.8

35.6

28

5 44.8 15

6.4 35

8

10

12.8

22.4 16

28

22.4

17.5

14

19.1

23.9

29.9

38.2

47.8

14.9

11.7

9.4

7.5

5.9

4.7

40

51.2

64

80

96

5.6

4.4

3.5

2.8

2.3

50

64

80

100

120

4.5

3.5

2.8

2.2

1.9

80

102.4

128

160

192

2.8

2.2

1.8

1.4

1.2

32

41

51.2

64

76.8

7

5.5

4.4

3.5

2.9

25

32

40

50

60

9

7

5.6 20

4.5 25

3.7

12.5 17.9 20

16

30

14

11.2

9

7.5

25.6

32

40

48

11.2

8.8

7

59.7

76.4

95.5

5.6 119.4

4.7 143.3

3.8

2.9

2.3

1.9

1.6

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

5

6*

15.8 15

20 11.9

19.7

25

12.1

9.5

31.5

40

25

31.3

9.5

7.6

31.3

39.1

7.6

6.1

50

62.5

40

50

62.5

80

5.9

4.8

3.8

3

50

62.5

78.1

100

4.8

3.8

3

2.4

80

100

125

160

100

125

150

2.4 125

1.9 156.3

1.6 187.5

1.9 200

1.5 250

1.3 300

2.5

3.2

4

5

6*

0.63

0.8

1

1.25

1.6

2

100

128

160

200

240

25.2

32

40

50

64

80

9.5

7.5

6

4.8

3.8

3

31.5

40

50

62.5

80

100

2.4 125

1.9 160

1.5 200

1.2 250

1 300

7.6 50.4

6

0.8

64

4.8 80

3.8 100

3 128

2.4 160

1.9 200

1.5 256

1.2 320

1 400

480

7.5 12.6 18.8

5.9 16 14.8

9.8

12.5

24.2

19

4.8 20

3.8 25

11.9

9.5

15.6

19.5

15.2

12.2

3 32

2.4 40

1.9 50

1.5 64

7.4

5.9

4.8

3.7

25

31.3

39.1

50

9.5

4.9

6.3

7.8

9.8

12.5

48.5

37.7

30.4

24.2

19

7.9

10

12.5

15.6

20

7.6 15.6 15.2 25

6.1 19.5 12.2 31.3

4.8 25 9.5 40

30.1

23.8

19

15.2

11.9

9.5

7.6

23.5

29.9

37.3

46.6

59.7

74.6

93.3

5.9 119.4

10.1

7.9

6.4

5.1

4

3.2

2.5

2

1.2 80

1 100

0.8 120

3 62.5

2.4 78.1

2 93.8

3.8 31.3

3 39.1

2.5 46.9

7.6 50

6.1 62.5

5.1 75

4.8 149.3

3.8 186.6

3.2 223.9

1.6

1.3

1.1

4.8 20.2 11.9 15.8 15.2

3.8 25.6 9.4 20 12

3 32

2.4 40

1.9 51.2

1.5 64

1.2 80

0.9 102.4

0.8 128

0.6 160

0.5 192

7.5

6

4.7

3.8

3

2.3

25

31.3

40

50

62.5

80

1.9 100

1.5 125

1.3 150

9.6

7.7

6

4.8 25

3.8

3

1.6

7.9

10

12.5

15.6

20

31.3

40

2.4 50

1.9 62.5

75

30.4

24

19.2

15.4

12

9.6

7.7

6

12.6

16

20

25

32

40

50

64

4.8 80

3.8 100

3.2 120

19

15

37.6

47.8

12 59.7

9.6 74.6

7.5 95.5

6 119.4

4.8 149.3

3.8 191

3 238.8

2.4 298.5

2 358.2

1.6

1.3

1

0.8

0.7

6.4

5

4

3.2

2.5

2

* Position 6 for MZ9 5 only

** When using the planachromatic and planapochromatic objectives MZ12 5 , the magnification is increased by the factor 1.25

×.


Objectives

Ergo objective

0.4

×–0.63×


63.5 mm 153.5mm

Eyepieces

10

16

×/21B

×/14B

25

×/9.5B

40 ×/6B

4

5

6*

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

5

2.5

3.2

6*

0.63

0.8

1

1.25

1.6

2

4

5

52.5

41.2

6.4 32.8

8.0 26.3

10.2 20.6

12.7 16.5

4

5

2.6 80.8

3.3 63.6

51.2

41.2

6.6 31.8

8.2 25.6

15.9 13.2 10.3 20.4

20.4 10.3 13.2 15.9

25.5

31.8

38.2

8.2

6.6

5.5

16.5

20.6

24.7

12.7

10.2

8.5

6.4 35

8.2 27.3

10.2 22

12.7 17.6

4 54.6

5.3 42.3

6.6 33.9

8.2 27.3

16.3 13.7 10.5 21.3

20.4 11 13.2 17

25.5

32.6

8.8 16.5 13.6

6.9 21 10.6

40.8

51

61

5.5 26.3

4.4 32.9

3.7 39.5

8.5

6.8

5.7

0.63

0.8

1

1.25

1.6

2

2.5

3.2

4

5

6*

4

5

6*

0.63

0.8

1

1.25

1.6

2

2.5

3.2

10 23.8

12.7 18.7

6.5 36.5

8.2 29

15.9 14.9 10.3 23

19.9 11.9 12.9 18.4

25.5

31.8

39.8

51

63.7

79.6

95.5

9.3 16.5 14.4

7.5 20.6 11.5

6

3.7

3

2.5

25.7

4.7 32.9

41.2

51.4

61.7

9.2

7.2

5.8

4.6

3.8

16 14.9 10.4 23

20.4 11.8 13.2 18.2

25.5

31.8

9.4

7.5

16.5

20.6

14.5

11.7

40.8

51

63.7

81.5

101.9

127.4

152.9

5.9

4.7

3.8

1.6

26.3

32.9

41.2

2.9 52.7

2.4 65.8

1.9 82.3

98.8

9

7.3

5.8

4.6

3.6

2.9

2.4


Optical data of Leica MZ12

5

, MZ16, MZ16 A

Objectives

1

× Plan

1

× Planapo

60 Plan

55 Planapo

0.5

× Plan

135 Plan

0.63

× Planapo

0.8

× Plan


97 Planapo 112 Plan

1.6

× Planapo

19 Planapo

Eyepieces

2

× Planapo

15 Planapo

10 ×/21B

16 ×/14B

25 ×/9.5B

40 ×/6B

16

20

25

31.5

40

50

57.5

3.5

4

5

6.3

8

10

12.5

5.7

6.4

8

10

12.8

16

20

25.6

32

40

50.4

64

80

92

8.9

10

12.5

15.6

20

25

31.3

40

50

62.5

78.8

100

125

143.8

80

100

126

160

200

230

14.2

16

20

25

32

40

50

64

6.6

5.3

4.2

3.3

2.6

2.1

1.8

29.6

26.3

21

16.8

13.1

10.5

8.4

19.7

17.5

14

11.2

8.8

7

5.6

4.4

3.5

2.8

2.2

1.8

1.4

1.2

13.4

11.9

9.5

7.6

5.9

4.8

3.8

3

2.4

1.9

1.5

1.2

1

0.8

8.4

7.5

6

4.8

3.8

3

2.4

1.9

1.5

1.2

1

0.8

0.6

0.5

32

40

50

63

80

100

115

7.1

8

10

12.5

16

20

25

11.4

12.8

16

20

25.6

32

40

51.2

64

80

100.8

128

160

184

17.7

20

25

31.3

40

50

62.5

80

100

125

157.5

200

250

287.5

28.4

32

40

50

64

80

100

128

160

200

252

320

400

460

3.2

4

5

6.3

8

10

11.5

0.71

0.8

1

1.25

1.6

2

2.5

0.71

0.8

1

1.25

1.6

2

2.5

3.2

4

5

6.3

8

10

11.5

0.71

0.8

1

1.25

1.6

2

2.5

3.2

4

5

6.3

8

10

11.5

4

5

6.3

8

10

11.5

0.71

0.8

1

1.25

1.6

2

2.5

3.2

20.5

25.6

32

40.3

51.2

64

72.5

4.5

5.1

6.4

8

10.2

12.8

16

7.2

8.2

10.2

12.8

16.4

20.5

25.6

32.8

41

51.2

64.5

81.9

102.4

115.9

11.1

12.8

16

20

25.6

32

40

51.2

64

80

100.8

128

160

181.1

17.9

20.5

25.6

32

41

51.2

64

81.9

102.4

128

161.3

204.8

256

289.8

13.1

10.5

8.4

6.7

5.3

4.2

3.7

59.1

52.5

42

33.3

26.3

21

16.8

39.4

35

28

22.4

17.5

14

11.2

8.8

7

5.6

4.4

3.5

2.8

2.4

26.8

23.8

19

15.2

11.9

9.5

7.6

5.9

4.8

3.8

3

2.4

1.9

1.7

16.9

15

12

9.6

7.5

6

4.8

3.8

3

2.4

1.9

1.5

1.2

1

25.6

32

40

50.4

64

80

92

8

10

5.7

6.4

12.8

16

20

9.1

10.2

12.8

16

20.5

25.6

32

41

51.2

64

80.6

102.4

128

147.2

32

40

50

64

14.2

16

20

25

80

100

126

160

200

230

22.7

25.6

32

40

51.2

64

80

102.4

128

160

201.6

256

320

368

47

41.2

32.8

26.3

20.6

16.4

13.1

10.2

8.2

6.6

5.2

4.1

3.3

2.9

31.3

27.3

22

17.5

13.7

10.9

8.8

6.8

5.5

4.4

3.5

2.7

2.2

1.9

21.2

18.6

14.8

11.9

9.3

7.4

5.9

4.6

3.7

3

2.4

1.9

1.5

1.3

13.4

11.7

9.4

7.5

5.9

4.7

3.8

2.9

2.3

1.9

1.5

1.2

0.9

0.8

* Zoom positions 0.71 and 11.5 only for MZ16/MZ16 A

4.1

3.3

2.6

2.1

1.6

1.3

1.1

18.5

16.4

13.1

10.5

8.2

6.6

5.3

12.3

10.9

8.8

7

5.5

4.4

3.5

2.7

2.2

1.8

1.4

1.1

0.9

0.8

3.7

3

2.4

1.9

8.4

7.4

5.9

4.8

1.5

1.2

0.9

0.7

0.6

0.5

2.3

1.9

1.5

1.2

5.3

4.7

3.8

3

0.9

0.8

0.6

0.5

0.4

0.3

11.4

12.8

16

20

25.6

32

40

51.2

64

80

100.8

128

160

184

18.2

20.5

25.6

32

41

51.2

64

81.9

102.4

128

161.3

204.8

256

294.4

28.3

32

40

50

64

80

100

128

160

200

252

320

400

460

45.4

51.2

64

80

102.4

128

160

204.8

256

320

403.2

512

640

736

8.2

6.6

5.3

4.2

3.3

2.6

2.2

37

32.8

26.3

21

16.4

13.1

10.5

24.6

22

17.5

14

10.9

8.8

7

5.5

4.4

3.5

2.8

2.2

1.8

1.5

16.7

14.8

11.9

9.5

7.4

5.9

4.8

3.7

3

2.4

1.9

1.5

1.2

1

10.5

9.4

7.5

6

4.7

3.8

3

2.3

1.9

1.5

1.2

0.9

0.8

0.6

64

80

100

126

160

200

230

14.2

16

20

25

32

40

50

22.8

25.6

32

40

51.2

64

80

102.4

128

160

201.6

256

320

368

80

100

125

160

35.4

40

50

62.5

200

250

315

400

500

575

320

400

504

640

800

920

56.8

64

80

100

128

160

200

256

3.3

2.6

2.1

1.7

1.3

1.1

0.9

14.8

13.1

10.5

8.4

6.6

5.3

4.2

4.4

3.5

2.8

2.2

1.8

1.4

9.8

8.8

7

5.6

1.1

0.9

0.7

0.6

3

2.4

1.9

1.5

6.7

5.9

4.8

3.8

1.2

1

0.8

0.6

0.5

0.4

1.9

1.5

1.2

0.9

4.2

3.8

3

2.4

0.8

0.6

0.5

0.4

0.3

0.3


Dimensions of Leica MS5

with incident-light stand with transmitted-light stand

1

× Achromat

Measurements in mm

1 × Achromat

MS5

MS5

Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency.


Dimensions of Leica MZ6


1 × Achromat

Measurements in mm

MZ6

1

× Achromat

MZ6




5


1

× Plan

Measurements in mm

MZ7

5

1 × Plan

MZ7

5




5


1 × Plan

Measurements in mm

MZ9

5

1 × Plan

MZ9

5




5


1

× Plan

Measurements in mm

MZ12

5

1 × Plan

MZ12

5





1x Planapo

1x Planapo

Measurements in mm

MZ16

MZ16



Dimensions of Leica MZ16 A


Measurements in mm



Technical data electronic devices

Transmitted-light base 20 W – Technical Data

Type description

Power supply

Type of bulb

Socket

Operating voltage

Fuses, primary

Switch positions

Transmitted light base 20 W

ES 635 474 incl. potentiometer

Halogen bulb 6 V/20 W

Connection for one external incident illumination with a maximum combined loading of 20 W

Primary voltage: 115 /-230 V, 50/60 Hz

Secondary voltage: 7 V 28 VA

For 115 V: 315 m AT / for 230 V: 160 m AT

Position I: Transmitted light 6 V/20 W

Position II: External incident lamp 20 W

Operating environmental conditions:

– Ambient temperature

– Relative humidity

– Atmospheric pressure

Transport and storage:

– Temperature

– Relative humidity


Protection class

Symbols

+ 10° C ... + 35° C

Ambient temperature up to 35° C: 75%

700 ... 1060 hPa

–20° C ... + 52° C

10 ... 95 % (non condensing)

500 ... 1200 hPa

Class II

Insulation, Protection class II

Safety transformer, protection class III

SEV mark of conformity, reference number OG 2

Regulating transformer – Technichal data

Model code

Outlet

Operating voltage

Transformer MTR38

2 × Connection for an external incident illuminator up to 20 W

Primary: 100–240 VAC, 47–63Hz, 1.8 A

Secondary: 5.3 V DC–7.5V/40 VA

2 × T 1A L 250 V Primary fuse:

Ambient conditions during operation:

– Ambient temperatures

– Rel. humidity


Transport and storage:

– Temperature

– Rel. humidity


Protection class

Symbols

+ 10° C ... + 35° C

Up to 35° C ambient temperature: 75%

700 ... 1060 hPa

–20° C ... + 52° C

10 ... 95 % (non-condensing)

500 ... 1200 hPa

IP 30 according to EN 60529/IEC 529


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Instruments. Yet Leica symbolizes innovation as well as tradition.

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Group operate internationally in four business segments, where we rank with the market leaders.

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