M o l e c u l a r S p e c t r o S c o p y

Thermo Scientific

DXR Raman Microscope

For sample microanalysis at or below the 1-micron level

The Thermo Scientific

™

DXR

™

Raman microscope is designed to produce the results demanded by Raman experts with the ease required by busy analytical laboratories. SmartLock optical components and a patented autoalignment system make set-up easy, fast and precise. Automated calibration, focus and exposure ensure quality results with every measurement.

The DXR Raman microscope delivers exceptional spatial resolution, together with the reliability, performance and reproducibility required in demanding analytical environments.

Designed for versatility, the DXR Raman microscope makes it as easy as possible to get high quality results.

• Class I laser-safe design for use in non-restricted environments

• Up to three excitation wavelengths for optimal results with demanding samples

• Patented autoalignment system for highest optimal performance

• Rapid, automated, multi-point calibration for confidence in sample identification

•

•

•

Patented autoexposure and autofocus for true point and shoot Raman spectroscopy

• 1 µm x, y spatial resolution and 2 µm depth resolution

• Laser Power Regulator to assure reproducible laser power at sample

True confocal design

Superb visual quality

• Compatible with a full range of high-quality

Olympus ® microscope components

General System Features

Lasers Single Laser Beam Path

Multiple Excitation Lasers

Laser Safety

Laser Power Regulator

Replaceable Smart Components

Components

SmartLock Components

Computer Interface

Fiber Optic Port

Supported wavelengths 780 nm, 633 nm, 532 nm

Class 1 standard. Class IIIb when fiber optic interface installed and on some specialized accessories.

Active feedback system to control absolute laser power delivered to the sample.

Facilitates laser-to-laser and system-to-system reproducibility.

Components (lasers, filters, gratings, fiber port) store and report to Thermo Scientific ™

OMNIC ™ serial number, component identity, lifetime usage (lasers only) and parameters for calibration (gratings only)

Pre-aligned, user-exchangeable system components (lasers, filters, gratings, fiber port) lock into place and automatically optimize system alignment and calibration upon installation

Through single USB 2.0 connector. Some accessories may require additional USB or serial ports.

Optional and user-installable

System Performance – Spectral Range and Resolution

Full-Range Grating Spectral Resolution 1

Upper Cutoff (cm -1 )

Lower Cutoff (cm -1 ) 2

High-Resolution Grating Spectral Resolution

Upper Cutoff (cm -1 )

Lower Cutoff (cm -1 ) 2

Extended-Range Grating Spectral Resolution

Upper Cutoff (cm -1 )

Lower Cutoff (cm -1 ) 2

532 nm

5.0 cm -1 FWHM

3500 cm -1

50 cm -1

2 cm -1 FWHM

1800 cm -1

50 cm -1

11 cm -1 FWHM

6000 cm -1

50 cm -1

633 nm

5.0 cm -1 FWHM

3500 cm -1

50 cm -1

2 cm -1 FWHM

1800 cm -1

50 cm -1

Lasers

780 nm

(high brightness)

5.0 cm

3300 cm

50 cm

2 cm

1. The system spectral resolution is measured using ASTM Method E 2529 – 06 and a 100 × objective.

The difference between system spectral resolution and spectrograph resolution is primarily determined by the excitation laser bandwidth.

-1

1800 cm

50 cm

-1

-1

FWHM

-1

FWHM

-1

-1

2. 50% maximum transmitted power. The use of some accessories such as fiber optic probes may reduce the spectral range. Check accessory specifications.

3. 780 nm high-power laser recommended for fiber optic measurements only. Not recommended for microscope work.

780 nm 3

(high power)

6.0 cm -1 FWHM

3300 cm -1

50 cm -1

Additional Performance Specifications

Spatial Resolution (x, y axes)

Wavenumber Accuracy 6

4

Sensitivity (Signal to Noise Ratio) 5

Reproducibility after Changing Excitation Lasers 7

Smallest sample that can be analyzed (independent of stage) <1 µm

Resolution with low-resolution motorized stage 3 µm

Resolution with high-precision motorized stage

532 nm laser

1 µm, diffraction-limited

3000:1

633 nm laser

780 nm high brightness laser

Wavenumber axis

900:1

750:1

±2 cm -1

1.8 cm -1 standard deviation with full-range grating

4. Spatial resolution depends on the wavelength of the excitation laser and the objective used.

The DXR Raman microscope achieves these specifications with a 100 × objective and with all three standard lasers.

The spatial resolution achieved also depends on the resolution of the motorized stage.

5. Average signal-to-noise ratio (peak height/RMS noise) measured using standard polystyrene, full-range grating.

15-sec measurement time, 5 mW laser power at sample, 25 µm slit aperture.

6. Wavenumber accuracy is measured with full-range grating and without changing lasers or gratings.

Accuracy is based on measurement of polystyrene using peaks at 1001.4, 1602.3, and 3054.3 cm -1 .

7. Over 10 numbers of exchanges, without recalibration or realignment.

Lasers

General Multiple Lasers

Laser Exchange

Optional

Lasers are pre-aligned and userexchangeable, no tools required.

Laser may be exchanged and shared between any DXR Raman instruments.

System Alignment

Smart Lasers

Automatically optimized upon exchange

Laser stores wavelength, serial number, lifetime usage

SmartLock Installation Precision-locked into place

Laser Power Regulator Power regulated in 0.1 mW increments. Power at sample is controlled by Laser Power Regulator and reported in mW.

Filtering All lasers include laser line filters to prevent laser artifacts from showing up in measured spectra

Depolarization All lasers are depolarized.

Minimizes orientation dependence in measurements.

532 nm Laser Laser Type

Laser Output Power

Diode-pumped, solid state (DPSS)

Maximum power at sample 10 mW

Lifetime

High Brightness

Warranty for 12 months

Yes

Center Wavelength 532 ± 1 nm

Transverse Mode TEM

00

Beam Quality (M

633 nm Laser Laser Type

2 ) <1.3

HeNe gas

Laser Output Power

Lifetime

Maximum power at sample 8 mW

Warranty for 12 months

High Brightness Yes

Center Wavelength 632.8 nm

Transverse Mode TEM

00

Beam quality (M 2 ) <1.2

780 nm

(high brightness)

Laser Type Frequency-stabilized single mode diode laser

Laser Output Power

Lifetime

High Brightness

Maximum power at sample 24 mW

Warranty for 12 months

Yes

Center Wavelength 780 ± 0.2 nm

Transverse Mode TEM

00

Beam Quality (M 2 ) <1.5

780 nm Laser Laser Type

(high power)

Multiple transverse mode, narrow-spectrum diode

Laser Output Power

High Brightness

Lifetime

Beam Quality (M 2

Maximum power at sample 150 mW

No

Warranty for 12 months

Center Wavelength 780 ± 0.5 nm

Spectral Bandwidth <0.2 nm

) N/A

Gratings

General

Filters

General

Installation

SmartLock Installation Grating is precision-locked into place

Smart Technology Grating stores serial number, wavelength, spectral resolution and calibration parameters

Grating Options

Fixed position, pre-aligned, user exchangeable without the need for tools

Full-range grating and high-resolution gratings available for each standard excitation wavelength

Installation Pre-aligned, user-exchangeable without the need for tools

SmartLock Installation Filter block is precision-locked into place

Smart Technology Filters store serial number and wavelength

Rayleigh Filters Stokes only

Fiber Optic Port

General Installation Pre-aligned, user-installable/ removable without the need for tools

SmartLock Installation Fiber optic port is precision-locked into place

Compatibility Compatible with all three standard excitation laser frequencies

Smart Technology

Accepts probes with standard

FC connectors

Fiber optic port stores serial number and identity

Spectrograph

Design Triplet Spectrograph No moving parts

Spectral Range Absolute 400–1050 nm

Spectral Full-range Grating

Dispersion

Average 2 cm -1 per CCD pixel element

High-resolution Average 1 cm -1 per CCD pixel

Grating element

Aperture Four 25 and 50 µm pinhole confocal

Software-selectable apertures; 25 and 50 µm slit

Apertures apertures

DXR Raman microscope is patented by US Patents 6,661,509 B2, 7,460,229 B2,

7,345,760 B2, 7,471,390 B2, 7,233,870 B1 and 7,605,918 B2. Other patents pending.

Microscope

Microscope

Stages Manual Stage

Standard Motorized Stage

Infinity-corrected confocal optics

2" × 3" travel X and Y dimensions

Manual Z focus control

5" × 3" travel X and Y dimensions

Step size 1 µm

Software-controlled Z focus

Joystick controller with focus control knob

High-precision Motorized Stage 4" × 3" travel X and Y dimensions

Stage Well-plate Adapter

Illuminator Brightfield Microscope

Options Illuminator, plus Nosepiece

Step size 0.1 µm

Software-controlled Z focus

Joystick controller with focus control knob

Accepts up to 1,536-well microtiter plates

Reflection illumination.

Accepts brightfield objectives

Brightfield/Darkfield

Microscope illuminator, plus Nosepiece

Reflection illumination. Accepts brightfield/darkfield objectives, plus brightfield-only objectives, with adaptor. Supports optional transmission illumination.

4 ×, 10×, 20×, 50×, 100× Objectives Standard Working

Distance Objectives

Long Working Distance Objectives 10 ×, 20×, 50×, 100×

Oil Immersion Objectives 50 ×, 100×

Macro Sampling Adapter

User-supplied Objective

Includes 4 × objective, accepts brightfield objectives

Must be compatible with Olympus BF or BD nosepieces 8

Microscopy Options Reflected Light Polarized Light Kit/Fixed Analyze

Reflected Light DIC Illumination Kit

8. Note that some objectives may be Raman-active and may contribute artifacts to the sample Raman spectrum. Objectives offered by Thermo Scientific have been tested to ensure there is no significant interference with sample measurement.

OMNIC Software Suite and User Interface

Smart Components

User Interface Autoexposure

OMNIC checks for laser, grating, filter compatibility

OMNIC restores alignment and calibration settings when lasers exchanged

Patented. Optimizes exposure time and number of exposures to deliver spectra with user-determined signal-to-noise ratio.

Autofocus

Smart Background

Optimizes signal from sample

Patented. Collected when instrument is not in use.

Eliminates the need for the user to collect backgrounds.

Automated Intensity Correction Consistent instrument response with all excitation lasers

Laser Power Regulator Absolute excitation laser power at the sample controlled by OMNIC

Automatic Fluorescence

Correction

Laser power at sample reported in mW

Compensates for fluorescence prior to data analysis; available for 532, 633 and 780 nm excitation wavelengths

Reported by OMNIC Excitation Laser Spot

Size at Sample

Specialty Thermo Scientific ™ OMNIC ™ Automated data collection and post collection data

Software Array ™ Automation Software analysis from multi-well plates and similar array formats

Thermo Scientific ™ OMNIC ™ Provides software-controlled hyperspectral mapping

Atlµs ™ Software and image analysis

OMNIC Series Software Supports time-evolved data collection

Thermo Scientific ™ OMNIC ™ Interface for advanced Visual Basic programming

Macros\Pro ™ Software

Instrument Alignment and Calibration

Alignment Entirely Software- Patented autoalignment technique controlled aligns laser, Raman emission and visual beam paths to microscope cross-hairs

Calibration 9 Wavelength

Calibration

Software-controlled calibration using multiple neon emission lines

Laser Frequency Software-controlled calibration using

Calibration multiple polystyrene Raman peaks

Intensity

Calibration

Software-controlled calibration using standardized white light sources

9. Standards incorporated into patented Alignment/Calibration Tool

Optional System Validation

Thermo Scientific ™ Available for the DXR Raman microscope

ValPro ™

Qualification

DQ documentation for hardware and software

Comprehensive IQ and OQ procedures in the industry-standard format

Full system qualification

OQ tests based on industry-standard methods

Serialized and traceable standard

Software for performance verification tests

Support for user-specific OQ or PQ tests

Instrument Serviceability

Replacement Lasers

Laser Lifetime

User-installable

Laser monitors number of hours of use

Laser Power Regulator Reports when laser power at sample has fallen below laser specification

Additional Laser, Filter, User-installable

Grating Sets

Physical Dimensions

Spatial Dimensions

Other Specifications

Environmental

Power Requirements

Regulatory Approval

Warranty Information

Width: 97 cm

Depth: 69 cm

Height: 61 cm

Weight: 56.7 kg

Minimum temperature: 16 °C

Maximum temperature: 27 °C

Humidity range: 20–80%

100–250 VAC, 48–63 Hz, 3.2 A max.

CE, UL/CSA/ETL, 21CFR1040.10

12-month warranty standard on the complete DXR Raman Microscope.

Extended warranties are available.

The DXR Raman microscope is a Class IIIb laser-safe product, unless installed with the Class I Laser Safety Enclosure. Installation of a fiber optic probe launcher and fiber probe will convert all microscopes to Class IIIb laser-safe, even with the Laser Safety Enclosure installed.

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