Hanna Instruments HI83399-01 Owner Manual

Multiparameter
Photometer with COD
INSTRUCTION MANUAL
HI83399
Dear
Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of the
instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of
the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
TABLE OF CONTENTS
1. PRELIMINARY EXAMINATION............................................................................................. 7
2. SAFETY MEASURES........................................................................................................... 7
3. SPECIFICATIONS............................................................................................................... 8
4. DESCRIPTION................................................................................................................... 9
4.1. GENERAL DESCRIPTION............................................................................................. 9
4.2. PRECISION AND ACCURACY........................................................................................ 9
4.3. FUNCTIONAL DESCRIPTION........................................................................................ 10
4.4. PRINCIPLE OF OPERATION......................................................................................... 11
4.5. OPTICAL SYSTEM...................................................................................................... 12
5. GENERAL OPERATIONS...................................................................................................... 13
5.1. POWER CONNECTION AND BATTERY MANAGEMENT...................................................... 13
5.2. GENERAL SETUP........................................................................................................ 13
5.3. USING HANNA DIGITAL ELECTRODES........................................................................... 16
5.4. MODE SELECTION..................................................................................................... 16
5.5. LOGGING DATA......................................................................................................... 17
5.6. ADDING SAMPLE / USER NAMES TO LOG DATA............................................................ 17
5.7. DATA MANAGEMENT.................................................................................................. 18
5.8. CONTEXTUAL HELP.................................................................................................... 19
6. PHOTOMETER MODE......................................................................................................... 19
6.1. METHOD SELECTION................................................................................................. 19
6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS.............................................. 20
6.2.1. PROPER USE OF SYRINGE................................................................................... 20
6.2.2. PROPER USE OF DROPPER................................................................................... 20
6.2.3. PROPER USE OF POWDER PACKET......................................................................... 20
6.3. CUVETTE PREPARATION............................................................................................. 21
6.4. USING THE 16 mm VIAL ADAPTER.............................................................................. 22
6.5. TIMERS AND MEASUREMENT FUNCTIONS................................................................... 24
6.6. CHEMICAL FORMULA / UNIT CONVERSIONS................................................................ 24
6.7. METER VALIDATION / CAL CHECK................................................................................ 25
6.8. ABSORBANCE MEASUREMENTS.................................................................................. 25
7. PROBE MODE................................................................................................................... 26
7.1. pH CALIBRATION...................................................................................................... 26
7.2. pH CALIBRATION MESSAGES...................................................................................... 28
7.3. pH MEASUREMENT................................................................................................... 28
7.4. pH MEASUREMENT MESSAGES / WARNINGS............................................................... 30
7.5. pH GLP..................................................................................................................... 31
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TABLE OF CONTENTS
4
7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE..................................................... 32
8. METHOD PROCEDURES..................................................................................................... 34
8.1. ALKALINITY............................................................................................................... 34
8.2. ALKALINITY, MARINE................................................................................................. 36
8.3. ALUMINUM.............................................................................................................. 38
8.4. AMMONIA LOW RANGE.............................................................................................. 41
8.5. AMMONIA LOW RANGE (16 mm VIAL)........................................................................ 44
8.6. AMMONIA MEDIUM RANGE........................................................................................ 47
8.7. AMMONIA HIGH RANGE............................................................................................. 50
8.8. AMMONIA HIGH RANGE (16 mm VIAL)....................................................................... 53
8.9. BROMINE................................................................................................................. 56
8.10. CALCIUM................................................................................................................ 58
8.11. CALCIUM, MARINE.................................................................................................. 61
8.12. CHLORIDE.............................................................................................................. 63
8.13. CHLORINE DIOXIDE................................................................................................. 67
8.14. CHLORINE DIOXIDE, RAPID METHOD......................................................................... 70
8.15. CHLORINE, FREE..................................................................................................... 74
8.16. CHLORINE, FREE ULTRA LOW RANGE......................................................................... 77
8.17. CHLORINE, TOTAL................................................................................................... 79
8.18. CHLORINE, TOTAL ULTRA LOW RANGE....................................................................... 82
8.19. CHLORINE, TOTAL ULTRA HIGH RANGE...................................................................... 84
8.20. CHROMIUM(VI) LOW RANGE................................................................................... 86
8.21. CHROMIUM(VI) HIGH RANGE.................................................................................. 88
8.22. CHEMICAL OXYGEN DEMAND LOW RANGE (16 mm VIAL)........................................... 90
8.23. CHEMICAL OXYGEN DEMAND MEDIUM RANGE (16 mm VIAL)..................................... 93
8.24. CHEMICAL OXYGEN DEMAND HIGH RANGE (16 mm VIAL).......................................... 96
8.25. COLOR OF WATER................................................................................................... 99
8.26. COPPER LOW RANGE............................................................................................... 101
8.27. COPPER HIGH RAGE................................................................................................ 103
8.28. CYANURIC ACID...................................................................................................... 105
8.29. FLUORIDE LOW RANGE............................................................................................ 107
8.30. FLUORIDE HIGH RANGE........................................................................................... 109
8.31. HARDNESS, CALCIUM.............................................................................................. 112
8.32. HARDNESS, MAGNESIUM........................................................................................ 115
8.33. HARDNESS, TOTAL LOW RANGE................................................................................ 118
8.34. HARDNESS, TOTAL MEDIUM RANGE.......................................................................... 121
TABLE OF CONTENTS
8.35. HARDNESS, TOTAL HIGH RANGE............................................................................... 124
8.36. HYDRAZINE............................................................................................................ 127
8.37. IODINE.................................................................................................................. 129
8.38. IRON LOW RANGE................................................................................................... 131
8.39. IRON HIGH RANGE.................................................................................................. 134
8.40. IRON(II)............................................................................................................... 136
8.41. IRON(II)/(III)........................................................................................................ 140
8.42. IRON, TOTAL (16 mm VIAL)..................................................................................... 144
8.43. MAGNESIUM.......................................................................................................... 148
8.44. MANGANESE LOW RANGE........................................................................................ 150
8.45. MANGANESE HIGH RANGE....................................................................................... 153
8.46. MOLYBDENUM....................................................................................................... 156
8.47. NICKEL LOW RANGE................................................................................................ 159
8.48. NICKEL HIGH RANGE............................................................................................... 162
8.49. NITRATE................................................................................................................ 164
8.50. NITRATE (16 mm VIAL)........................................................................................... 167
8.51. NITRITE, MARINE ULTRA LOW RANGE....................................................................... 170
8.52. NITRITE LOW RANGE............................................................................................... 172
8.53. NITRITE HIGH RANGE.............................................................................................. 175
8.54. NITROGEN, TOTAL LOW RANGE (16 mm VIAL)........................................................... 177
8.55. NITROGEN, TOTAL HIGH RANGE (16 mm VIAL).......................................................... 183
8.56. OXYGEN, DISSOLVED............................................................................................... 188
8.57. OXYGEN SCAVENGERS (CARBOHYDRAZIDE)............................................................... 191
8.58. OXYGEN SCAVENGERS (DIETHYLHYDROXYLAMINE) (DEHA)........................................ 194
8.59. OXYGEN SCAVENGERS (HYDROQUINONE)................................................................. 197
8.60. OXYGEN SCAVENGERS (ISO-ASCORBIC ACID)............................................................ 200
8.61. OZONE................................................................................................................... 203
8.62. pH........................................................................................................................ 207
8.63. PHOSPHATE, MARINE ULTRA LOW RANGE................................................................. 209
8.64. PHOSPHATE LOW RANGE......................................................................................... 211
8.65. PHOSPHATE HIGH RANGE........................................................................................ 213
8.66. PHOSPHORUS, REACTIVE LOW RANGE (16 mm VIAL)................................................ 216
8.67. PHOSPHORUS, REACTIVE HIGH RANGE (16 mm VIAL)............................................... 217
8.68. PHOSPHORUS, ACID HYDROLYZABLE (16 mm VIAL).................................................. 222
8.69. PHOSPHORUS, TOTAL LOW RANGE (16 mm VIAL)..................................................... 226
8.70. PHOSPHORUS, TOTAL HIGH RANGE (16 mm VIAL).................................................... 230
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TABLE OF CONTENTS
6
8.71. POTASSIUM........................................................................................................... 234
8.72. SILICA LOW RANGE................................................................................................. 237
8.73. SILICA HIGH RANGE................................................................................................ 240
8.74. SILVER.................................................................................................................. 243
8.75. SULFATE................................................................................................................ 247
8.76. SURFACTANTS, ANIONIC.......................................................................................... 249
8.77. ZINC...................................................................................................................... 253
9. WARNINGS & ERRORS...................................................................................................... 256
10. STANDARD METHODS..................................................................................................... 259
11. ACCESSORIES................................................................................................................ 262
11.1. REAGENT SETS....................................................................................................... 262
11.2. pH ELECTRODES..................................................................................................... 267
11.3. pH SOLUTIONS...................................................................................................... 268
11.4. OTHER ACCESSORIES.............................................................................................. 269
12. ABBREVIATIONS............................................................................................................ 271
CERTIFICATION.................................................................................................................... 272
RECOMMENDATIONS FOR USERS.......................................................................................... 275
WARRANTY......................................................................................................................... 275
PRELIMINARY EXAMINATION
1. PRELIMINARY EXAMINATION
Remove the instrument and accessories from the packaging and examine it carefully to make sure
that no damage has occurred during shipping. Notify your nearest Hanna Customer Service Center if
damage is observed.
Each HI83399 is supplied with:
• Sample Cuvette and Cap (4 pcs.)
• Cloth for Wiping Cuvettes
• Scissors
• USB Cable
• 5 Vdc Power Adapter
• 16 mm Vial Adapter
• 16 mm diameter Vial Cuvette with cap (6 pcs.)
• Instruction Manual
• DO bottle (glass stopper bottle)
• Quality Certificate
Note: Save all packing material until you are sure that the instrument works correctly.
Any damaged or defective item must be returned in its original packing material with the
supplied accessories.
SAFETY MEASURES
2. SAFETY MEASURES
•The chemicals contained in the reagent kits may be hazardous if improperly
handled.
•Read the Safety Data Sheets (SDS) before performing tests.
• Safety equipment: Wear suitable eye protection and clothing when required, and follow
instructions carefully.
• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If
reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released
vapors.
• Waste disposal: for proper disposal of reagent kits and reacted samples, contact a licensed
waste disposal provider.
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SPECIFICATIONS
3. SPECIFICATIONS
Measurement Channels
Absorbance
Range
Resolution
Accuracy
Light Source
Bandpass Filter Bandwidth
5 x optical channels;
1 x digital electrode channel (pH measurement)
0.000 to 4.000 Abs
0.001 Abs
±0.003 Abs (at 1.000 Abs)
light emitting diode
8 nm
Bandpass Filter Wavelength Accuracy ±1.0 nm
pH
Light Detector
Cuvette Types
Number of Methods
Range
Resolution
Accuracy
Temperature Compensation
Calibration
Temperature
Additional
Specifications
Electrode
Range
Resolution
Accuracy
Logging
Display
USB-A (Host) Functions
USB-B (Device) Functions
Battery Life
Power Supply
Environment
Dimensions
Weight
silicon photocell
round, 24.6 mm diameter and 16 mm diameter
82
-2.00 to 16.00 pH (± 1000.0 mV)*
0.01 pH (0.1 mV)
±0.01 pH (±0.2 mV) (@ 25 °C / 77 °F)
ATC (-5.0 to 100.0 °C; 23.0 to 212.0 °F)*
2 points, eligible from 5 available buffers (4.01,
6.86, 7.01, 9.18, 10.01 pH)
Intelligent pH / temperature electrode
-20.0 to 120.0°C (-4.0 to 248.0 °F)
0.1 °C (0.1 °F)
±0.5 °C (±0.9 °F) (@ 25 °C / 77 °F)
1000 readings (mixed photometer and electrode)
128 x 64 pixel B/W LCD with backlight
mass-storage host
power input, mass-storage device
> 500 photometer measurements, or 50 hours of
continuous pH measurement
5 Vdc USB 2.0 power adapter/type micro-B connector
3.7 Vdc Li-polymer rechargeable battery,
non-serviceable
0 to 50 °C (32 to 122 °F);
0 to 95% RH, non-serviceable
206 x 177 x 97 mm (8.1 x 7.0 x 3.8")
1.0 kg (2.2 lbs.)
*Limits will be reduced to actual probe/sensor limits.
8
DESCRIPTION
4. DESCRIPTION
4.1.GENERAL DESCRIPTION
HI83399 multiparameter photometer is compact and versatile meter with two measurement
modes: Absorbance and pH/ mV. Absorbance mode include CAL Check feature and 82 different
methods that cover a wide variety of applications, making it ideal for both benchtop and portable
operation.
• Digital electrode input for pH measurements
• Certified CAL Check cuvettes to confirm meter functionality
• Dual purpose micro-USB flash drive
• Li-polymer rechargeable battery
• Auto-off
• Absorbance mode
• User and sample name entry
• GLP features
4.2.PRECISION AND ACCURACY
Precision is how closely repeated measurements
are to one another. Precision is usually expressed
as standard deviation (SD).
Accuracy is defined as the closeness of a test
result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure
explains these definitions.
For each method, the accuracy is expressed in the
related measurement section.
9
DESCRIPTION
4.3. FUNCTIONAL DESCRIPTION
1)
2)
3)
4)
5)
6)
7)
8)
9)
10)
10
Splash-proof keypad
Liquid Crystal Display (LCD)
Indexing mark
Protective port covers
Light-blocking cover panel
Cuvette holder
ON/OFF power button
3.5-mm TRRS (jack) input for digital electrodes
Standard USB host connector for data transfer to a USB flash drive
Micro-USB device connector for power or PC interface
Press the functional keys to perform the function displayed above them on the LCD.
Press to access the list of photometer methods.
Press to move up in a menu or a help screen, to increment a set value, or to access
second level functions.
DESCRIPTION
Keypad Description
The keypad contains 12 direct keys and 3 functional keys with the following functions:
Press to toggle between photometer and pH (electrode) mode.
Press to move left in a menu or to increment a set value.
Press to move down in a menu or a help screen, to decrement a set value, or to access
second level functions.
Press to move right in a menu or to increment a set value.
Press to access the setup screen.
Press to log the current reading.
Press to review saved logs.
Press to exit the current screen.
Press to display the help screen.
ON/OFF power button.
4.4. PRINCIPLE OF OPERATION
Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical-chemical characteristics of the substance according to the
Lambert-Beer Law:
-log I/Io = el c d
or
A = el c d
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DESCRIPTION
= intensity of incident light beam
Io
I
= intensity of light beam after absorption
= molar extinction coefficient at wavelength λ
el
c
= molar concentration of the substance
d
= optical path through the substance
Therefore, the concentration "c" can be calculated from the absorbance of the substance as the
other factors are constant.
Photometric chemical analysis is based on specific chemical reactions between a sample and
reagent to produce a light-absorbing compound.
4.5. OPTICAL SYSTEM
Instrument Block Diagram
The internal reference system (reference detector) of the HI83399 photometer compensates for any
drifts due to power fluctuations or ambient temperature changes, providing a stable source of light
for your blank (zero) measurement and sample measurement.
LED light sources offer superior performance compared to tungsten lamps. LEDs have a much higher
luminous efficiency, providing more light while using less power. They also produce little heat,
which could otherwise affect electronic stability. LEDs are available in a wide array of wavelengths,
whereas tungsten lamps have poor blue/violet light output.
Improved optical filters ensure greater wavelength accuracy and allow a brighter, stronger signal to
be received. The end result is higher measurement stability and less wavelength error.
A focusing lens collects all of the light that exits the cuvette, eliminating errors from cuvette
imperfections and scratches, eliminating the need to index the cuvette.
12
- battery is charging from external adapter- battery fully charged (meter connected to AC/DC adapter)
GENERAL OPERATIONS
5. GENERAL OPERATIONS
5.1. POWER CONNECTION AND BATTERY MANAGEMENT
The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery.
The meter will perform an auto-diagnostic test when it is first powered on. During this test, the
HANNA® logo will appear on the LCD. After 5 seconds, if the test was successful, the last method
used will appear on the display.
The battery icon on the LCD will indicate the battery status:
- battery capacity (no external adapter)- battery near 0% (no external adapter)
- battery exhausted (no external adapter)
To conserve battery, the meter will turn off automatically after 15 minutes of inactivity (30 minutes
before a READ measurement). If a photometer measurement is on the screen, an auto-log is
created before shutdown.
5.2. GENERAL SETUP
Press SETUP key to enter in Setup menu, highlight desired option using
and press Select.
CAL Check (Photometer Only)
Press Select to enter the CAL Check screen. The date, time and
values for the last CAL Check are displayed on the screen.
To start a new CAL Check press Check key and follow the prompts
on the screen.
13
GENERAL OPERATIONS
Temperature Unit (pH Only)
Option: °C or °F
Press the functional key to select the desired temperature unit.
Backlight
Values: 0 to 8
Press the Modify key to access the backlight intensity.
Use the functional keys or the   keys to increase or decrease
the value.
Press the Accept key to confirm or ESC to return to the Setup
menu without saving the new value.
Contrast
Values: 0 to 20
Press the Modify key to change the display’s contrast.
Use the functional keys or the   keys to increase or decrease
the value.
Press the Accept key to confirm the value or ESC to return to the
Setup menu without saving the new value.
Date / Time
Press the Modify key to change the date/time.
Press the functional keys or the   keys to highlight the value
to be modified (year, month, day, hour, minute or second).
Use the   keys to change the value.
Press the Accept key to confirm or ESC to return to the Setup
without saving the new date or time.
Time Format
Option: AM/PM or 24-hour
Press the functional key to select the desired time format.
14
Decimal Separator
Option: Comma ( , ) or Period ( . )
Press the functional key to select the desired decimal separator.
The decimal separator is used on the measurement screen and
CSV files.
GENERAL OPERATIONS
Date Format
Press the Modify key to change the Date Format.
Use the   keys to select the desired format.
Press the Select key to confirm or ESC to return to the Setup menu
without saving the new format.
Language
Press the Modify key to change the Language. Use the  
keys to select the desired language. Press Select to change the
language.
Press the functional key to select one of the 7 languages installed.
Beeper
Option: Enable or Disable
When enabled, a short beep is heard every time a key is pressed.
A long beep alert sounds when the pressed key is not active or an
error is detected. Press the functional key to enable/disable the
beeper.
Instrument ID
Option: 0 to 999999
This option is used to set the instrument’s ID (identification
number). Press the Modify key to access the instrument ID screen.
Use the functional keys or the  keys to highlight the digit
to be modified. Press the   keys in order to set the desired
value. Press the Accept key to confirm the value or ESC to return
to the Setup menu without saving the new value.
15
GENERAL OPERATIONS
Meter Information
Press the Select key to view the model, serial number, firmware
version and selected language. Press ESC to return to the Setup
menu.
Probe Information (pH mode only)
Press the Select key to view model number, serial number and
firmware version for the connected probe.
Press ESC to return to the Setup menu.
5.3. USING HANNA DIGITAL ELECTRODES
The HI83399 can be used to perform direct pH measurements by connecting a HANNA® digital
pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the
electrode to the 3.5 mm port marked with “EXT PROBE” located at the rear of the meter. If the
meter is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.
5.4. MODE SELECTION
The HI83399 has two operational modes: Photometer Mode and Probe Mode.
Photometer Mode enables on-demand measurement of a cuvette using the integrated optical
system. Photometric-related functions, such as Method selection, Zero, Read, and Timers are
available in this mode.
Probe Mode enables continuous measurement using a Hanna Digital Electrode connected to the
3.5 mm port. Probe-related functions, such as calibration and GLP, are available in this mode.
To switch between Photometer Mode and Probe Mode, use the
button.
Note: The active mode cannot be switched while in menus, such as Setup, Recall, Method, etc.
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GENERAL OPERATIONS
5.5. LOGGING DATA
The instrument features a data log function to help you keep track of all your analysis. The data log
can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using
the LOG and RECALL keys.
Storing data: You can store only a valid measurement. Press LOG and the last valid
measurement will be stored with date and time stamp.
5.6. ADDING SAMPLE / USER NAMES TO LOG DATA
A sample ID and user ID can be added to the saved log. Use the  keys to highlight the
Sample ID or User ID then press Modify.
Text Entry
Sample ID and User ID care entered using the alphanumeric multi-tapping keypad.
Enter one character at a time by pressing the key with the assigned character repeatedly until the
desired character is highlighted. For reference, a list of the characters available for the current key
will be shown under the text box.
The character will be entered after a two-second delay or after another key is pressed.
Once all characters have been entered, press Accept to use the displayed text.
17
GENERAL OPERATIONS
The following functions are available during Text Entry:
• Accept: Press to accept the current displayed text.
• Arrow: Press to delete the last character.
• Clear: Press to delete all characters.
Press to discard all changes and return to the previous screen.
5.7. DATA MANAGEMENT
Viewing and deleting: You can view, export and delete the data by pressing the RECALL key. Use
the  keys to scroll through the saved logs. Press Info to view additional information about
the selected log.
Data Export:
Log data can be exported to a USB flash drive or to a PC. To access Data Export functions, press
Recall then Export.
Use the   keys to select the desired export location.
For export to USB Flash Drive, insert the USB Flash Drive into the dedicated port at the back of the
meter labeled HOST USB, then follow the on-screen prompts.
For export to PC, connect the meter to a PC using the supplied micro-USB cable. Insert the cable
into the port at the back of the meter labeled PC PWR. Follow the on-screen prompts. When the
meter says PC connected, use a file manager (such as Windows Explorer or Mac Finder) to move the
file from the meter to the PC. The meter will appear as a removable disk.
18
5.8. CONTEXTUAL HELP
HI83399 offers an interactive contextual help mode that assists the user at any time.
To access the help screen press HELP.
The instrument will display additional information related to the
current screen. To read all the available information, scroll the text
using the   keys.
To exit help mode press ESC key and the meter will return to the
previous screen.
PHOTOMETER MODE
6. PHOTOMETER MODE
6.1. METHOD SELECTION
In order to select the desired method press the METHOD key and a screen with the available
methods will appear.
Press the  keys to highlight the desired method. Press Select.
GENERAL OPERATIONS
Log data is exported as a single file containing all logged photometer and probe data. The file
name is: “HI83399.csv”. The CSV file (Comma-Separated Values) may be opened with a text
editor or spreadsheet application.
After the desired method is selected, follow the procedure described in the related section.
Before performing a method read all the instructions carefully.
19
PHOTOMETER MODE
6.2. COLLECTING AND MEASURING SAMPLES AND REAGENTS
6.2.1. PROPER USE OF SYRINGE
(a) Push the plunger completely into the syringe and insert the tip
into the solution.
(b) Pull the plunger up until the lower edge of the seal is exactly on
the mark for the desired volume.
(c) Take out the syringe and clean the outside of the syringe tip, be
sure that no drops are hanging on the tip of the syringe. Then,
keeping the syringe in vertical position above the cuvette, push
the plunger down into the syringe, the desired volume has been
delivered into the cuvette.
6.2.2. PROPER USE OF DROPPER
(a) For reproducible results, tap the dropper on the table several times and wipe the outside of the
tip with a cloth.
(b) Always keep the dropper bottle in a vertical position while dosing the reagent.
6.2.3. PROPER USE OF POWDER PACKET
(a) Use scissors to open the powder packet
(b) Push the edges of the packet to form a spout
(c) Pour out the content of the packet.
20
PHOTOMETER MODE
6.3. CUVETTE PREPARATION
Proper mixing is very important for reproducibility of the measurements. The proper mixing
technique for each method is listed in the method procedure.
(a) Invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical
position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end,
then return the cuvette to the upright vertical position and wait for all of the solution to flow to
the cuvette bottom. This is one inversion. The correct speed for this mixing technique is
10-15 complete inversions in 30 seconds.
This mixing technique is indicated with “invert to mix” and the following icon:
(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or
vigorous. This mixing method is indicated with “shake gently” or “shake vigorously”, and one
of the following icons:
shake gently
shake vigorously
In order to avoid reagent leaking and to obtain more accurate measurements,
and then the
close the cuvette first with the supplied HDPE plastic stopper
black cap.
Whenever the cuvette is placed into the measurement holder, it must be dry
outside and free of fingerprints, oil or dirt. Wipe it thoroughly with HI731318 or
a lint-free cloth prior to insertion.
Shaking the cuvette can generate bubbles in the sample, causing higher
readings. To obtain accurate measurements, remove such bubbles by swirling or
by gently tapping the cuvette.
21
PHOTOMETER MODE
Do not let the reacted sample stand too long after reagent is added. For best accuracy, respect the
timings described in each specific method.
It is possible to take multiple readings in a row, but it is recommended
to take a new zero reading for each sample and to use the same cuvette
for zeroing and measurement when possible.
Discard the sample immediately after the reading is taken, or the glass
might become permanently stained.
All the reaction times reported in this manual are at 25 °C (77 °F). In
general, the reaction time should be increased for temperatures lower
than 20 °C (68 °F), and decreased for temperatures higher than 25 °C
(77 °F).
Interference
In the method measurement section the most common interferences that may be present in a
typical water sample have been reported. It is possible that a particular application could introduce
other compounds that will also interfere.
6.4. USING THE 16mm VIAL ADAPTER
Some parameters require special single-use 16 mm vials. These parameters can be identified by
the "(16)" in the method name and the appearance of "16 mm" on the measurement screen.
To prepare the meter for use with 16 mm vials:
1. Lift open the meter’s sample cover.
2. Orient the vial adapter with the six small holes toward the bottom.
3. Orient the vial adapter with the indexing mark toward the left. This indexing mark should align
with the indexing mark located on the meter.
4. Insert the vial slowly into the cuvette holder of the meter
keeping the index marks on the adapter and meter aligned
with each other. If the adapter appears blocked, the adapter
may need to be rotated slightly in order to correctly engage
the guides in the meter’s cuvette holder.
22
PHOTOMETER MODE
5. Using light pressure, push the adapter down until it reaches the bottom of the meter’s cuvette
holder. When the vial adapter reaches the bottom, you should no longer be able to see the
notched area of the adapter.
6. The meter is ready for use with 16 mm vial parameters. Always use the vial adapter for both
“Zero” and “Read” measurements as specified in the parameter instructions.
Note :The meter’s sample cover will not close completely while using the vial adapter.
This is normal – the vial adapter itself will block out external light.
WARNING: Improper use of the 16 mm vial adapter could cause irreversible damage to the
meter. Always use the following precautions while using the 16 mm vial adapter:
• Never use excessive force to insert the adapter. You should be able to insert the vial with light
pressure using one finger. If the vial is not reaching the bottom, if there is large resistance, or
if you are receiving a “light low” error during the “Zero” operation, re-check that the indexing
marks are aligned on the adapter and meter.
• Never insert hot vials/samples into the vial adapter. Samples should be near room temperature
before inserting into the meter/adapter.
• Do not attempt to close the sample cover while using the 16 mm vials or adapter. It is normal
for the vials/adapter to prevent the cover from closing completely.
23
PHOTOMETER MODE
6.5. TIMERS AND MEASUREMENT FUNCTIONS
Each method requires a different preparation procedure, reaction times, sample preparations, etc. If
a timer or timers are necessary for proper sample preparation, the Timer key will be available.
To use a reaction timer, press the Timer key.
The default timer will start immediately. To stop and reset the timer, press Stop.
If the selected method requires more than one timer, the meter will automatically select each timer
in the appropriate order. To bypass the default order, you may press the desired key to activate a
different timer (only while the current timer is stopped). Press Continue to start the active timer.
For some methods, the timer is only necessary after a Zero measurement has been performed. In
this case, the timer key will only be available after the Zero measurement has been performed.
If the method requires a Zero or Read measurement after a timer has expired, the meter will
automatically perform the appropriate action. Follow the instructions in the Method Procedure.
To perform a Zero or Read measurement, insert the appropriate prepared cuvette, then press the
Zero or Read key. A Zero measurement must be conducted before Read measurements.
6.6. CHEMICAL FORMULA / UNIT CONVERSION
Chemical formula/unit conversion factors are pre-programmed into the instrument and are method
specific. In order to view the displayed result in the desired chemical formula press  keys to
access the second level function and then press the Chem Frm key to toggle between the available
chemical formulas for the selected method.
24
WARNING: Do not validate the meter with standard solutions other than the HANNA® CAL Check
Standards. For accurate validation results, please perform tests at room temperature (18 to 25 °C;
64.5 to 77.0 °F).
Validation of the HI83399 involves absorbance measurements of certified HANNA® CAL Check
Standards (see “Accessories”). The “CAL Check” screen guides the user through the measurement of
each CAL Check Standard and applies the factory calibration corrections to each measurement. The
HI83399 stores the results of the most recent CAL Check measurements which may be viewed on
the “CAL Check” screen. Compare these results with the values printed on the Certificate provided
with each HANNA® CAL Check Standards kit.
To perform a validation:
PHOTOMETER MODE
6.7. METER VALIDATION / CAL CHECK
1. Press Setup button.
2. Highlight CAL Check, then press Select.
3. Follow the prompts on the screen. The meter will prompt to
measure each cuvette provided in the HANNA® CAL Check
Standards kit. To abort the process at any time, press ESC
button.
4. Press ESC to return in Setup menu.
6.8. ABSORBANCE MEASUREMENTS
Raw absorbance measurements may be performed on the HI83399 for personal or diagnostic
purposes. For example, you may monitor the stability of a reagent blank by occasionally measuring
its absorbance versus deionized water.
To measure the raw absorbance of a prepared sample:
1. Enable “Photometer Mode” if necessary by pressing the MODE key.
2. Press the METHOD key.
25
PHOTOMETER MODE
PROBE MODE
3. Highlight the appropriate Absorbance method (according to the wavelength to be used), then
press Select. Absorbance methods are located at the bottom of the method list.
4. Prepare the sample cuvette according to the method.
5. Insert a cuvette filled with deionized water, then press Zero.
6. Insert the prepared sample cuvette, then press Read.
WARNING: Never use Absorbance methods for validation using HANNA® CAL Check cuvettes. The
factory calibration corrections for CAL Check cuvettes are applied while in CAL Check mode only!
7. PROBE MODE
7.1. pH CALIBRATION
Press MODE to enter in pH/ mV measurement mode.
Press Calibrate to access electrode calibration functions.
Calibration Mode
While in pH Calibration Mode, the display will show the current pH reading, the current
temperature reading, the current selected buffer, and the buffer number (“Buffer: 1” for the
1st buffer, “Buffer: 2” for the 2nd buffer).
The following functions are available in pH Calibration Mode:
• Clear: Press to clear the current calibration from the probe.
• Confirm: Press to accept the current calibration point. Only available if the measurement is
stable and within the limits for the selected buffer.
26
Press to exit calibration and return to pH Measurement Mode.
PROBE MODE
Press to cycle through the list of available buffers: 4.01, 6.86, 7.01, 9.18, 10.01 pH.
Preparation
Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to
minimize any EMC interferences. For accurate calibration and to minimize cross-contamination, use
two beakers for each buffer solution: one for rinsing the electrode and one for calibration. If you are
measuring in the acidic range, use pH 7.01 or 6.86 as the first buffer and pH 4.01 as the second
buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as the first buffer and
pH 10.01 or 9.18 as the second buffer.
Procedure
Calibration can be performed using one or two calibration buffers. For more accurate
measurements, a two-point calibration is recommended.
Submerse the pH electrode approximately 3 cm (1¼”) into a buffer solution and stir gently. From
the Probe Measurement screen, press the Calibrate key to begin the calibration process.
When the reading is stable and close to the selected buffer, the Confirm key will become available.
Press Confirm to accept and store the calibration point.
The meter will now prompt for the second buffer (“Buffer: 2”). To use only a one-point calibration,
to exit calibration mode at this time. The meter will store the calibration information
press
to the probe and return to Measurement mode. To continue calibrating with a second buffer, rinse
and submerse the pH electrode approximately 3 cm (1¼”) into the second buffer solution and stir
gently. If necessary, press
keys to select a different buffer value.
When the reading is stable and close to the selected buffer, the Confirm key will become available.
Press Confirm to accept and store the second calibration point.
The meter will store the two-point calibration information to the probe and return to Measurement
mode. The list of calibrated buffers will appear at the bottom of the screen.
27
PROBE MODE
7.2. pH CALIBRATION MESSAGES
Clean Probe:
The "Clean Probe" message indicates poor electrode
performance (offset out of accepted window, or slope under the
accepted lower limit). Often, cleaning the probe will improve
the pH electrode's response. See pH Electrode Conditioning and
Maintenance for details. Repeat calibration after cleaning.
Check Probe & Buffer:
The “Check Probe & Buffer” message appears when there
is a large difference between the pH measurement and the
selected buffer value, or the electrode slope is outside of the
accepted slope limit. You should check your probe and confirm
the correct buffer selection. Cleaning may also improve this
response.
Wrong Temperature:
The buffer temperature is too extreme for the selected buffer value.
7.3. pH MEASUREMENT
The HI83399 can be used to perform direct pH measurements by connecting a HANNA® digital
pH electrode with a 3.5 mm TRRS connector. To begin taking probe measurements, connect the
electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter
is in “Photometer Mode”, set the meter to “Probe Mode” by pressing the MODE key.
While taking pH probe measurements, the following functions are available:
• Calibrate: Press to access electrode calibration functions.
• GLP: Press to review the last calibration information, including date/time, buffers used, slope,
and offset.
• Range: Press to switch between “pH” units and “mV” units.
28
Press to access the meter’s Setup menu.
Press to log the current measurement.
Press to review the meter’s log history.
PROBE MODE
Press to switch to Photometer mode.
Press to view contextual help information.
For high accuracy it is recommended to calibrate your electrode often. pH electrodes should be
recalibrated at least once per week, but daily calibration is recommended. Always recalibrate after
cleaning an electrode. See page 26 for more information on pH calibration.
To take pH measurements:
• Remove the protective cap and rinse the electrode with water.
• Collect some sample in a clean, dry beaker.
• Preferably, rinse the electrode with a small amount of sample. Discard the rinse.
• Submerse the electrode tip approximately 3 cm (1¼”) into the sample to be tested and stir the
sample gently. Make sure the electrode junction is completely submersed.
symbol disappears, your
• Allow time for the electrode to stabilize in the sample. When the
reading is stable.
If measurements are taken successively in different samples, it is recommended to rinse the
electrodes thoroughly with deionized or distilled water and then with some of the next sample to
prevent cross-contamination.
pH measurements are affected by temperature. HANNA® Digital pH electrodes include a built-in
temperature sensor and automatically calculate corrected pH values. The measured temperature is
displayed on the screen with the pH measurements.
29
PROBE MODE
7.4. pH MEASUREMENT MESSAGES / WARNINGS
No Probe:
No probe is connected or the probe is broken.
Connecting:
The meter has detected a probe and is reading the probe
configuration and calibration information.
Incompatible Probe:
The connected probe is not compatible with this device.
Incompatible Calibration:
The probe’s current calibration is not compatible with this
meter. The calibration must be cleared to use this probe.
Exceeded Probe Range:
The pH and/or temperature measurement exceed the
specifications of the probe. The affected measurement value(s)
will be flashing.
Broken Temperature Sensor:
The temperature sensor inside the probe is broken.
Temperature compensation will revert to a fixed value of
25 °C (77 °C).
Cal Due:
The probe has no calibration. See section Probe Calibration.
30
PROBE MODE
7.5. pH GLP
Good Laboratory Practice (GLP) refers to a quality control function used to ensure uniformity and
consistency of sensor calibrations and measurements. To view the GLP information, press the GLP
key from the Probe Measurement screen.
The pH GLP screen displays the following information about the last pH calibration:
• Date and time of the last calibration
• List of buffers used in the last calibration
• Calculated slope and offset
• Press ESC to return in measurement mode.
31
PROBE MODE
7.6. pH ELECTRODE CONDITIONING AND MAINTENANCE
Remove the protective cap of the pH electrode.
DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT.
This is normal with electrodes. They will disappear when rinsed with water.
During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of
the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with
a glass thermometer. If the bulb and/or junction is dry, soak the electrode in HI70300 or HI80300
storage solution for at least one hour.
For refillable electrodes:
If the fi lling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI7082 or
HI8082 3.5M KCl Electrolyte Solution for double junction electrodes.
Unscrew the fill hole cover during measurements so the liquid reference junction maintains
an outward flow of electrolyte.
Measurement
Rinse the electrode tip with distilled water. Submerse the tip 3 cm (1¼”) in the sample and stir
gently for a few seconds. For a faster response and to avoid cross-contamination of the samples, rinse
the electrode tip with a few drops of the solution to be tested, before taking measurements.
32
PROBE MODE
Storage Procedure
To minimize clogging and ensure a quick response time, the glass bulb and the junction should be
kept moist and not allowed to dry out.
Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage
Solution or, in its absence, Filling Solution (HI7082 or HI8082 for double junction electrodes).
Follow the preparation procedure before taking measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
Periodic Maintenance
Inspect the electrode and the cable. The cable used for connection to the instrument must be intact
and there must be no points of broken insulation on the cable or cracks on the electrode stem or
bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the
electrode. Rinse off any salt deposits with water.
For refillable electrodes: Refill the reference chamber with fresh electrolyte (HI7082 or HI8082 for
double junction electrodes). Allow the electrode to stand upright for 1 hour.
Follow the Storage Procedure above.
Cleaning Procedure
Use diagnostic messages to aid pH electrode troubleshooting. Several cleaning solutions are
available:
• General – Soak in Hanna HI7061 or HI8061 General Cleaning Solution for
approximately ½ hour.
• Protein –Soak in Hanna HI7073 or HI8073 Protein Cleaning Solution for 15 minutes.
• Inorganic – Soak in Hanna HI7074 Inorganic Cleaning Solution for 15 minutes.
• Oil/grease – Rinse with Hanna HI7077 or HI8077 Oil and Fat Cleaning Solution.
Note: After performing any of the cleaning procedures, rinse the electrode thoroughly with
distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel- filled
electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least
1 hour before taking measurements.
Temperature Correlation For pH Sensitive Glass
Verify the temperature range by reading the limits on electrodes cap. The pH electrode’s life also
depends on the temperature that is used. If constantly cycled between two temperatures, the life of
the electrode is drastically reduced.
33
ALKALINITY
8. METHOD PROCEDURES
8.1. ALKALINITY
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 500 mg/L (as CaCO3)
1 mg/L
±5 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Colorimetric Method
REQUIRED REAGENTS
Code
Description Quantity
HI775S
Alkalinity Reagent
1 mL
HI93755-53
Chlorine Removal Reagent
1 drop
REAGENT SETS
Reagents for 25 tests
HI775-26
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Alkalinity method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
34
Note: Any chlorine present in the sample will interfere
with the reading. To remove the chlorine interference
add one drop of HI93755-53 Chlorine Removal Reagent
to the unreacted sample.
ALKALINITY
• Remove the cuvette.
• Add 1mL of HI775S Alkalinity Reagent to the sample
using a 1 mL syringe.
• Replace the cap and invert 5 times.
• Reinsert the cuvette into the instrument and close the lid.
• Press Read to start the reading. The instrument displays the results in mg/L calcium carbonate
(CaCO3).
35
ALKALINITY, MARINE
8.2. ALKALINITY, MARINE
SPECIFICATIONS
Range0 to 300 mg/L (as CaCO3)
Resolution1 mg/L
Accuracy±5 mg/L ±5% of reading at 25 °C
Light SourceLED with narrow band interference filter @ 610 nm
MethodColorimetric Method
REQUIRED REAGENTS
Code
Description
HI755S
Alkalinity Reagent
Quantity
1 mL
REAGENT SETS
HI755-26
Reagents for 25 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Alkalinity Marine method using the procedure described in
the Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
36
• Add 1 mL of HI755S Alkalinity Reagent to the sample
using a 1mL syringe.
ALKALINITY, MARINE
• Remove the cuvette.
• Replace the cap and gently invert 5 times.
• Reinsert the cuvette into the instrument and close the lid.
• Press Read to start the reading. The instrument displays the results in mg/L as calcium
carbonate (CaCO3).
• Press  or  to access the second level functions.
• Press Chem Frm key to convert the result to degree KH (dKH).
• Press  or  to return to the measurement screen.
37
ALUMINUM
8.3. ALUMINUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 1.00 mg/L (as Al3+)
0.01 mg/L
±0.04 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the aluminon method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93712A-0
Aluminum Reagent A
1 packet
HI93712B-0
Aluminum Reagent B
1 packet
HI93712C-0
Aluminum Reagent C
1 packet
REAGENT SETS
HI93712-01
Reagents for 100 tests
HI93712-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Aluminum method using the procedure described in the
Method Selection section (see page 19).
• Fill a graduated beaker with 50 mL of sample.
• Add one packet of HI93712A-0 Aluminum Reagent A and mix until
completely dissolved.
• Add one packet of HI93712B-0 Aluminum Reagent B and mix until
completely dissolved.
38
• Add one packet of HI93712C-0 Aluminum Reagent C to
one cuvette (#1). Replace the cap and shake gently until
completely dissolved. This is the blank.
10 mL
10 mL
#1
#2
ALUMINUM
• Fill two cuvettes with 10 mL of sample (up to the mark).
#1
• Place the first cuvette (#1) into the holder and close the lid.
#1
• Press Timer and the display will show the countdown prior to zeroing the blank. Alternatively
wait for 15 minutes and then press Zero. The display will show “-0.0-“ when the meter is
zeroed and ready for measurement.
• Remove the blank and insert the second cuvette (#2) into the
instrument and close the lid.
#2
39
ALUMINUM
• Press the Read key to start the reading. The instrument displays the results in mg/L of
aluminum (Al3+).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of aluminum oxide (Al2O3).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Iron above 20 mg/L
Alkalinity above 1000 mg/L
Phosphate above 50 mg/L
Fluoride must be absent
40
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 3.00 mg/L (as NH3-N)
0.01 mg/L
±0.04 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426 Nessler method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93700A-0
Ammonia Low Range Reagent A
4 drops
HI93700B-0
Ammonia Low Range Reagent B
4 drops
AMMONIA LOW RANGE
8.4. AMMONIA LOW RANGE
REAGENT SETS
HI93700-01
Reagents for 100 tests
HI93700-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Ammonia LR method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
41
AMMONIA LOW RANGE
• Add 4 drops of HI93700A-0 Ammonia Low Range Reagent A.
Replace the cap and mix the solution.
• Add 4 drops of HI93700B-0 Ammonia Low Range Reagent B.
Replace the cap and mix the solution.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH3-N).
42
• Press  or  to return to the measurement screen.
AMMONIA LOW RANGE
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium
(NH4+).
INTERFERENCE
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines
43
AMMONIA LOW RANGE (16 mm VIAL)
8.5. AMMONIA LOW RANGE (16 mm VIAL)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 3.00 mg/L (as NH3-N)
0.01 mg/L
± 0.10 mg/L or ± 5% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426 Nessler method
REQUIRED REAGENTS
Code
Description
Quantity
HI93764A-0*
Ammonia Low Range Reagent Vial
1 vial
HI93764-0
Nessler Reagent4 drops
*Reagent Vial identification: A LR, white label
Note: Store the unused vials in a cool and dark place.
REAGENT SETS
Reagents for 25 tests
HI93764A-25
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Ammonia LR (16) method following one of the procedures
described in the Method Selection section (see page 19).
• Insert the 16mm vial adapter using the procedure described in the
Using the 16mm Vial Adapter section (see page 22).
• Remove the cap from HI93764A-0 Ammonia Low Range Reagent
Vial.
• Add 5.0 mL of sample to the vial, while keeping the vial at a
45-degree angle.
• Replace the cap and invert several times to mix.
44
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.
• Remove the vial.
• Remove the cap and add 4 drops of HI93764-0 Nessler Reagent.
• Replace the cap and invert the vial several times to mix.
AMMONIA LOW RANGE (16 mm VIAL)
• Place the vial into the holder.
• Place the vial into the holder.
• Press Timer and the display will show the countdown prior to the measurement, or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L ammonia nitrogen (NH3-N).
45
AMMONIA LOW RANGE (16 mm VIAL)
46
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium
(NH4+).
• Press  or  to return to the measurement screen.
INTERFERENCES
Organic compounds like: chloramines, various aliphatic and aromatic amines, glycine or urea above
10 ppm (to eliminate these interferences distillation is required).
Organic compounds like: aldehydes, alcohols (e.g. ethanol), or acetone above 0.1%. (to eliminate
these interferences distillation is required).
Sulfide: may cause turbidity.
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 10.00 mg/L (as NH3-N)
0.01 mg/L
±0.05 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426, Nessler method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93715A-0
Ammonia Medium Range Reagent A
4 drops
HI93715B-0
Ammonia Medium Range Reagent B
4 drops
AMMONIA MEDIUM RANGE
8.6. AMMONIA MEDIUM RANGE
REAGENT SETS
HI93715-01
Reagents for 100 tests
HI93715-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Ammonia MR method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
47
AMMONIA MEDIUM RANGE
• Remove the cuvette.
• Add 4 drops of HI93715A-0 Ammonia Medium Range
Reagent A. Replace the cap and mix the solution.
• Add 4 drops of HI93715B-0 Ammonia Medium Range
Reagent B. Replace the cap and mix the solution.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results to mg/L of ammonia nitrogen (NH3-N).
• Press or to access the second level functions.
48
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines
AMMONIA MEDIUM RANGE
• Press the Chem Frm key to convert the result in mg/L of ammonia (NH3) and ammonium
(NH4+).
49
AMMONIA HIGH RANGE
8.7. AMMONIA HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 100.0 mg/L (as NH3-N)
0.1 mg/L
±0.5 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426, Nessler method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93733A-0
Ammonia High Range Reagent A
4 drops
HI93733B-0
Ammonia High Range Reagent B
9 mL
REAGENT SETS
HI93733-01
Reagents for 100 tests
HI93733-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Ammonia HR method using the procedure described in the
Method Selection section (see page 19).
1 mL
• Add 1mL of unreacted sample to the cuvette using 1mL
syringe.
• Use the pipette to fill the cuvette up to the 10 mL mark
with HI93733B-0 Ammonia High Range Reagent B.
Replace the cap and mix the solution.
• Place the cuvette into the holder and close the lid.
50
• Remove the cuvette.
• Add 4 drops of HI93733A-0 Ammonia High Range Reagent A.
Replace the cap and swirl the solution.
AMMONIA HIGH RANGE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of ammonia nitrogen (NH3-N).
• Press  or  to access the second level functions.
51
AMMONIA HIGH RANGE
•Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium
(NH4+).
•Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Acetone
Alcohols
Aldehydes
Glycine
Hardness above 1 g/L
Iron
Organic chloramines
Sulfide
Various aliphatic and aromatic amines
52
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 100.0 mg/L (as NH3-N)
0.1 mg/L
± 1.0 mg/L or ± 5% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 420 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426 Nessler method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93764B-0*
Ammonia High Range Reagent Vial
1 vial
HI93764-0
Nessler Reagent4 drops
*Reagent Vial identification: A HR, green label.
Note: Store the unused vials in a cool and dark place.
REAGENT SETS
Reagents for 25 tests
HI93764B-25
For other accessories see page 267.
AMMONIA HIGH RANGE (16 mm VIAL)
8.8. AMMONIA HIGH RANGE (16 mm VIAL)
MEASUREMENT PROCEDURE
• Select the Ammonia HR (16) method using the procedure described
in the Method Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in the
Using the 16 mm Vial Adapter section (see page 22).
• Remove the cap from HI93764B-0 Ammonia High Range Reagent
Vial.
• Add 1.0 mL of sample to the vial, while keeping the vial at a
45-degree angle.
• Replace the cap and invert several times to mix.
53
AMMONIA HIGH RANGE (16 mm VIAL)
• Place the vial into the holder.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the vial.
• Add 4 drops of HI93764-0 Nessler Reagent.
• Replace the cap and invert several times to mix.
• Place the vial into the holder.
• Press Timer and the display will show the countdown prior to the measurement, or, alternatively
wait 3 minutes and 30 seconds. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L ammonia nitrogen (NH3-N).
• Press  or  to access the second level functions.
54
•Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Organic compounds like: chloramines, various aliphatic and aromatic amines, glycine or urea above
100 ppm; to eliminate these interferences distillation is required.
Organic compounds like: aldehydes, alcohols (e.g. ethanol) or acetone above 1 %; to eliminate
these interferences distillation is required.
Sulfide: may cause turbidity.
AMMONIA HIGH RANGE (16 mm VIAL)
• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and ammonium
(NH4+).
55
BROMINE
8.9. BROMINE
SPECIFICATIONS
Range
0.00 to 8.00 mg/L (as Br2)
Resolution
0.01 mg/L
Accuracy
±0.08 mg/L ±3% of reading at 25 °C
Light Source
LED with narrow band interference filter @ 525 nm
Method
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, DPD method
REQUIRED REAGENTS
Code
Description
HI93716-0
Bromine Reagent
Quantity
1 packet
REAGENT SETS
HI93716-01
Reagents for 100 tests
HI93716-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Bromine method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
56
• Add one packet of HI93716-0 Bromine Reagent. Replace
the cap and shake gently for about 20 seconds to dissolve
most of the reagent.
BROMINE
• Remove the cuvette.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of bromine (Br2).
INTERFERENCES
Interference may be caused by: Chlorine, Iodine, Ozone, Oxidized forms of Chromium and Manganese.
In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately
1 minute after adding the reagent.
In case of water with alkalinity greater than 300 mg/L CaCO3 or acidity greater than 150 mg/L CaCO3,
the color of the sample may develop only partially, or may rapidly fade. To resolve this, neutralize the
sample with diluted HCl or NaOH.
57
CALCIUM
8.10. CALCIUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method 0 to 400 mg/L (as Ca2+)
1 mg/L
±10 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the Oxalate method
REQUIRED REAGENTS
CodeDescriptionQuantity
-
Buffer Reagent
4 drops
HI93752A-Ca
Calcium Reagent A
7 mL
HI93752B-Ca
Calcium Reagent B
1 mL
REAGENT SETS
Reagents for 50 tests
HI937521-01
HI937521-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Calcium method using the procedure described in the
Method Selection section (see page 19).
• Add 3 mL of unreacted sample to the cuvette using the 5 mL syringe.
• Use the pipette to fill the cuvette up to the 10 mL
mark with the HI93752A-Ca Calcium Reagent A.
• Add 4 drops of Buffer Reagent.
58
3 mL of sample
• Place the cuvette into the holder and close the lid.
CALCIUM
• Replace the cap and invert several times to mix.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add 1 mL of HI93752B-Ca Calcium Reagent B
to the sample using the 1 mL syringe. Invert the
cuvette 10 times to mix (about 15 seconds).
• Reinsert the cuvette into the instrument.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 5 minutes.
59
CALCIUM
• After waiting 5 minutes, invert again the cuvette 10 times
to mix (about 15 seconds).
• Reinsert the cuvette into the instrument and close the lid.
• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca2+).
INTERFERENCES
Interferences may be caused by:
Acidity (as CaCO3) above 1000 mg/L
Alkalinity (as CaCO3) above 1000 mg/L
Magnesium (Mg2+) above 400 mg/L
60
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method CALCIUM, MARINE
8.11. CALCIUM, MARINE
200 to 600 mg/L (as Ca2+)
1 mg/L
±6% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Adaptation of the Zincon method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI7581
Calcium Reagent A
1 mL
HI7582
Calcium Reagent B
1 packet
REAGENT SETS
HI758-26
Reagents for 25 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Calcium Marine method using the procedure described in
the Method Selection section (see page 19).
• Add 1 mL of HI7581 Calcium Reagent A to the cuvette
using a 1 mL syringe.
10 mL
• Use the plastic pipette to fill the cuvette to the 10 mL mark
with deionized water and replace the cap. Invert 3-5 times
to mix.
• Place the cuvette into the holder and close the lid.
61
CALCIUM, MARINE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Use the minipipette to add 0.1 mL of sample to the cuvette.
• Add one packet of HI7582 Calcium Reagent B. Replace
the cap and shake vigorously for 15 seconds or until
the powder is completely dissolved. Allow air bubbles to
dissipate for 15 seconds before taking a reading.
• Reinsert the cuvette into the instrument and close the lid.
• Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca2+).
62
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method 0.0 to 20.0 mg/L (as Cl-)
0.1 mg/L
±0.5 mg/L ±6% of reading at 25 °C
Light Emitting Diode with narrow band interference filter @ 466 nm
Adaptation of the mercury(II) thiocyanate method
CHLORIDE
8.12. CHLORIDE
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93753A-0
Chloride Reagent A
1 mL
HI93753B-0
Chloride Reagent B
1 mL
REAGENT SETS
HI93753-01
Reagents for 100 tests
HI93753-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chloride method using the procedure described in the Method Selection section (see
page 19).
• Fill one cuvette (#1) with 10 mL of deionized water (up to the mark).
#1
• Fill another cuvette (#2) with 10 mL of sample (up to the mark).
#2
10 mL
10 mL
Notes: • For samples with low chloride ion concentration, rinse the cuvette a few times
with sample before filling it with 10 mL of sample.
• For the most accurate results, use two graduated pipettes to deliver exactly10 mL
of deionized water and 10 mL of sample to the cuvettes.
63
CHLORIDE
• Add 0.5 mL of HI93753A-0 Chloride Reagent A to each
cuvette using the 1 mL syringe
#1
#2
#1
#2
• Replace the caps and mix each cuvette by inverting for
approximately 30 seconds.
• Add 0.5 mL of HI93753B-0 Chloride Reagent B to each
cuvette using the second 1 mL syringe.
• Replace the caps and mix each cuvette by inverting for
approximately 30 seconds.
• Place the cuvette with the reacted deionized water (#1)
into the holder and close the lid.
64
#1
• Remove the cuvette.
CHLORIDE
• Press Timer and the display will show the countdown prior to the zero or, alternatively, wait for
2 minutes and press Zero. The display will show "-0.0-" when the meter is zeroed and ready for
measurement.
#2
• Insert the other cuvette (# 2) with the reacted sample into
the instrument and close the lid.
• Press Read to start reading. The instrument displays the results in mg/L of chloride (Cl-).
65
CHLORIDE
66
INTERFERENCES
Interference may be caused by:
For alkaline samples, neutralize before adding reagents. The pH of the sample after addition of
reagents should be about 2.
Intensely colored samples will cause interference, therefore they should be adequately treated before
performing the test. Suspended matter in large amount should be removed by prior filtration.
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
CHLORINE DIOXIDE
8.13. CHLORINE DIOXIDE
0.00 to 2.00 mg/L (as ClO2)
0.01 mg/L
±0.10 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the Chlorophenol Red method
REQUIRED REAGENT
CodeDescriptionQuantity
HI93738A-0
Chlorine Dioxide Reagent A
1 mL
HI93738B-0
Chlorine Dioxide Reagent B
1 packet
HI93738C-0
Chlorine Dioxide Reagent C
1 mL
HI93738D-0
Chlorine Dioxide Reagent D
1 mL
REAGENT SETS
Reagents for 100 tests
HI93738-01
HI93738-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chlorine Dioxide method using the procedure
described in the Method Selection section
(see page 19).
• Fill two graduated mixing cylinders (#1 & #2) up to
the 25 mL mark with the sample.
#1
#2
25 ml
25 ml
• Add 0.5 mL of HI93738A-0 Chlorine Dioxide Reagent A to each cylinder (#1 & #2), using a
1 mL syringe, cap them and invert several times to mix.
#1 & #2
#1
#2
67
CHLORINE DIOXIDE
• Add one packet of HI93738B-0 Chlorine Dioxide Reagent B to one of the two cylinders (#1),
cap and invert it several times until it is totally dissolved. This is the blank.
#1
• Add 0.5 mL of HI93738C-0 Chlorine Dioxide Reagent C to each cylinder (#1 & #2), using a
1 mL syringe, cap, and invert several times to mix.
#1 & #2
#1
#2
• Add 0.5 mL of HI93738D-0 Chlorine Dioxide Reagent to each cylinder (#1 & #2), using a
1 mL syringe, cap and invert several times to mix. Cylinder #2 is the reacted sample.
#1 & #2
#1
10 mL
#1
#2
• Fill cuvette (#1) with 10 mL of the blank (up to the mark) and
replace the cap.
• Place the blank (#1) into the holder and close the lid.
68
#1
• Fill second cuvette (#2) with 10 mL of the reacted sample (up
to the mark) and replace the cap.
CHLORINE DIOXIDE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
#2
10 mL
• Insert the sample into the instrument and close the lid.
#2
• Press Read to start the reading. The instrument displays the results in mg/L of chlorine
dioxide (ClO2).
SAMPLING PROCEDURE
It is recommended to analyze chlorine dioxide samples immediately after collection. Chlorine
Dioxide samples must be stored in sealed dark glass bottle, with minimal head space. Excessive
heat (above 25 °C /77 °F), agitation and exposure to light must be avoided.
INTERFERENCES
Interferences may be caused by strong oxidants.
69
CHLORINE DIOXIDE, RAPID METHOD
8.14. CHLORINE DIOXIDE, RAPID METHOD
SPECIFICATIONS
Range
0.00 to 2.00 mg/L (as ClO2)
Resolution
0.01 mg/L
Accuracy
±0.10 mg/L ±5% of reading at 25 °C
Light Source
LED with narrow band interference filter @ 525 nm
MethodAdaptation of Standard Methods for the Examination of Water and
Wastewater,18th. ed., 4500 ClO2 D
REQUIRED REAGENT
CodeDescriptionQuantity
HI96779A-0
Chlorine Dioxide Reagent A
5 drops
HI96779B-0
Chlorine Dioxide Reagent B
1 packet
REAGENT SETS
HI96779-01
Reagents for 100 tests
HI96779-03
Reagents for 300 tests
For other accessories see page 267.
PRINCIPLE
The reaction between the Chlorine Dioxide and DPD indicator causes a pink tint in the sample;
the addition of glycine as a masking agent inhibits the response of free chlorine.
APPLICATION
Drinking water, tap water and treated water.
NOTE
Collect the sample in a clean glass bottle and analyze it immediately. Chlorine Dioxide is a strong
oxidizing agent and is unstable in waters.
70
MEASUREMENT PROCEDURE
• Select the Chlorine Dioxide (Rapid) method using the procedure described in the
Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark).
CHLORINE DIOXIDE, RAPID METHOD
SIGNIFICANCE AND USE
Chlorine dioxide is a commonly-used alternative to chlorine (Cl2) as a water disinfectant. The
Chlorophenol Red method (non-rapid method) reacts specifically with chlorine dioxide with little
interference from free chlorine or chloramines, but the method procedure is cumbersome. The
Chlorine Dioxide Rapid Method based on the DPD (N,N-diethyl-p-phenylenediamine) indicator is
a much simpler method by comparison, but it is susceptible to interference from other oxidizers.
Glycine (Reagent A) is able to convert free chlorine to chloroaminoacetic acid without affecting
the analysis of chlorine dioxide content.
• Add 5 drops of HI96779A-0 Chlorine Dioxide Reagent A.
• Replace the cap and shake gently for 30 seconds.
• Wait for 30 seconds.
71
CHLORINE DIOXIDE, RAPID METHOD
• Place the cuvette into the meter and close the lid.
• Press the Zero key. The display will show “-0-”; the meter is zeroed and ready for measurement.
• Remove the cuvette.
• Add one packet of HI96779B-0.
• Replace the cap and shake gently 20 seconds.
• Reinsert the cuvette into the meter and close the lid.
72
INTERFERENCES
Interferences may be caused by:
Acidity
Alkalinity
Bromine, Br2 above 0.1 mg/L
Chlorine, Cl2 above 5 mg/L
Inorganic chloramines
Chloramines, organic
Chromium, Oxidized (Cr6+)
Flocculating agents
Hardness
Manganese, Oxidized (Mn4+, Mn7+)
Metals
Monochloramine
Ozone
Peroxides
Highly buffered samples or extreme sample pH
CHLORINE DIOXIDE, RAPID METHOD
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of ClO2.
73
CHLORINE, FREE
8.15 CHLORINE, FREE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 5.00 mg/L (as Cl2)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the EPA DPD method 330.5
REQUIRED REAGENTS
POWDER:
CodeDescriptionQuantity
HI93701-0
Free Chlorine Reagent
1 packet
LIQUID:
CodeDescriptionQuantity
HI93701A-F
Free Chlorine Reagent A
3 drops
HI93701B-F
Free Chlorine Reagent B
3 drops
REAGENT SETS
Reagents for 300 tests (liquid)
HI93701-F
HI93701-01
Reagents for 100 tests (powder)
HI93701-03
Reagents for 300 tests (powder)
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chlorine (Free) method using the procedure described in
the Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
74
10 mL
CHLORINE, FREE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
POWDER REAGENT PROCEDURE
• Add the content of one packet of HI93701-0 Free Chlorine
Reagent. Replace the cap and shake gently for 20 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of chlorine (Cl2).
LIQUID REAGENT PROCEDURE
• To an empty cuvette add 3 drops of HI93701A-F Free
Chlorine Reagent A and 3 drops of HI93701B-F Free
Chlorine Reagent B.
75
CHLORINE, FREE
• Swirl gently to mix.
• Add 10 mL of unreacted sample (up to the mark).
Replace the cap and shake gently.
10 mL
• Insert the cuvette into the instrument and close the lid.
• Press Read to start the reading. The instrument displays the results in mg/L of chlorine (Cl2).
Note: Free and Total Chlorine have to be measured separately with fresh sample following
the related procedure if both values are desired.
INTERFERENCES
Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and
Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for
approximately 2 minutes after adding the powder reagent.
If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity
value greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may
rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.
76
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
CHLORINE, FREE ULTRA LOW RANGE
8.16. CHLORINE, FREE ULTRA LOW RANGE
0.000 to 0.500 mg/L (as Cl2)
0.001 mg/L
±0.020 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the Standard Method 4500-Cl G
REQUIRED REAGENTS
CodeDescriptionQuantity
HI95762-0
Free Chlorine Ultra Low Range Reagent
1 packet
REAGENTS SETS
HI95762-01
Reagents for 100 tests
HI95762-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chlorine Free ULR method using the procedure
described in the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
77
CHLORINE, FREE ULTRA LOW RANGE
• Add one packet of HI95762-0 Free Chlorine Reagent.
Replace the cap and shake gently for 20 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of chlorine (Cl2).
INTERFERENCES
Interference may be caused by:
Alkalinity: above 1,000 mg/L CaCO3 if present as bicarbonate (HCO3 sample pH < 8.3); above
25 mg/L CaCO3 if present as carbonate (CO32-, sample pH > 9.0). In both cases, it will not reliably
develop the full amount of color or it may rapidly fade (negative error). To resolve this, neutralize
the sample with diluted HCl.
Acidity: above 150 mg/L CaCO3. May not reliably develop the full amount of color or it may rapidly
fade (negative error). To resolve this, neutralize the sample with diluted NaOH.
Hardness: in case of water with hardness greater than 500 mg/L CaCO3, shake the sample for
approximately 2 minutes after adding the powder reagent.
Bromine (Br2), Chloride dioxide (ClO2), Iodine (I2), Oxidized Manganese and Chromium, Ozone
(O3): positive error.
78
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
CHLORINE, TOTAL
8.17. CHLORINE, TOTAL
0.00 to 5.00 mg/L (as Cl2)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the EPA DPD method 330.5
REQUIRED REAGENTS
POWDER:
CodeDescriptionQuantity
HI93711-0
Total Chlorine Reagent
1 packet
LIQUID:
CodeDescriptionQuantity
HI93701A-T
Total Chlorine Reagent A
3 drops
HI93701B-T
Total Chlorine Reagent B
3 drops
HI93701C-T
Total Chlorine Reagent C
1 drop
REAGENT SETS
HI93701-T
Reagents for 300 tests (liquid)
HI93711-01
Reagents for 100 total tests (powder)
HI93711-03
Reagents for 300 total tests (powder)
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chlorine (Total) method using the procedure described in
the Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
79
CHLORINE, TOTAL
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
POWDER REAGENT PROCEDURE
• Add 1 packet of HI93711-0 Total Chlorine Reagent. Replace the
cap and shake gently for 20 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of chlorine (Cl2).
LIQUID REAGENT PROCEDURE
• To an empty cuvette add 3 drops of HI93701A-T
Total Chlorine Reagent A, 3 drops of HI93701B-T
Total Chlorine Reagent B, and 1 drop of
HI93701C-T Total Chlorine Reagent C. Swirl gently
to mix.
80
10 mL
CHLORINE, TOTAL
• Add 10 mL of unreacted sample (up to the mark).
Replace the cap and shake gently.
• Insert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of chlorine (Cl2).
Note: Free and Total Chlorine have to be measured separately with fresh unreacted samples
following the related procedure if both values are desired.
INTERFERENCES
Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and
Manganese. In case of water with hardness greater than 500 mg/L CaCO3 shake the sample for
approximately 2 minutes after adding the powder reagent.
If the water used for this procedure has an alkalinity value greater than 250 mg/L CaCO3 or acidity
value greater than 150 mg/L CaCO3, the color of the sample may develop only partially, or may
rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH.
81
CHLORINE, TOTAL ULTRA LOW RANGE
8.18. CHLORINE, TOTAL ULTRA LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.000 to 0.500 mg/L (as Cl2)
0.001 mg/L
±0.020 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the EPA recommended Method 330.5-
REQUIRED REAGENTS
CodeDescriptionQuantity
HI95761-0
Total Chlorine Ultra Low Range Reagent
1 packet
REAGENT SETS
HI95761-01
Reagents for 100 tests
HI95761-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chlorine (Total) ULR method using the procedure
described in the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
82
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of chlorine (Cl2).
INTERFERENCES
Interference may be cased by:
Alkalinity: above 1,000 mg/L CaCO3 if present as bicarbonate (HCO3 sample pH < 8.3); above
25 mg/L CaCO3 if present as carbonate (CO32-, sample pH > 9.0). In both cases, it will not reliably
develop the full amount of color or it may rapidly fade (negative error). To resolve this, neutralize
the sample with diluted HCl.
Acidity: above 150 mg/L CaCO3. May not reliably develop the full amount of color or it may rapidly
fade (negative error). To resolve this, neutralize the sample with diluted NaOH.
Hardness: in case of water with hardness greater than 500 mg/L CaCO3, shake the sample for
approximately 2 minutes after adding the powder reagent.
Bromine (Br2), Ozone (O3) and Chlorine dioxide (ClO2): positive error.
CHLORINE, TOTAL ULTRA LOW RANGE
• Add one packet of HI95761-0 Total Chlorine Reagent.
Replace the cap and shake gently for 20 seconds.
83
CHLORINE, TOTAL ULTRA HIGH RANGE
8.19. CHLORINE, TOTAL ULTRA HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 500 mg/L (as Cl2)
1 mg/L
±3 mg/L ±3% of reading at 25 °C
LED lamp with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for Examination of Water and Wastewater, 20th edition, 4500-Cl
REQUIRED REAGENTS
CodeDescriptionQuantity
HI95771A-0
Total Chlorine Ultra High Range Reagent A 1 packet
HI95771B-0
Total Chlorine Ultra High Range Reagent B 1 packet
REAGENTS SETS
Reagents for 100 tests
HI95771-01
HI95771-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chlorine (Total) UHR method using the procedure
described in the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
84
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of chlorine (Cl2).
CHLORINE, TOTAL ULTRA HIGH RANGE
• Add one packet of HI95771A-0 Total Chlorine Ultra High
Range Reagent A and one packet HI95771B-0 Total
Chlorine Ultra High Range Reagent B. Replace the cap and
shake gently for 20 seconds.
INTERFERENCES
Interference may be caused by:
Bromine (Br2), Oxidized Manganese, Chromium, Chlorine Dioxide (ClO2), Ozone (O3) and Iodine (I2).
85
CHROMIUM(VI) LOW RANGE
8.20. CHROMIUM(VI) LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 300 µg/L (as Cr (VI))
1 µg/L
±10 µg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1687 Diphenylcarbohydrazide method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93749-0
Chromium(VI) Low Range Reagent
1 packet
REAGENT SETS
HI93749-01
Reagents for 100 tests
HI93749-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chromium(VI) LR method using the procedure described in
the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93749-0 Chromium(VI) Low Range
Reagent. Replace the cap and shake vigorously for about
10 seconds.
86
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in µg/L of chromium (Cr6+).
CHROMIUM(VI) LOW RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of chromate (CrO42¯) and dichromate
(Cr2O72¯).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by: Vanadium above 1 ppm. However, waiting 10 minutes before
reading removes the interference. Iron above 1 ppm. Mercurous and mercuric ions cause slight
inhibition of the reaction.
87
CHROMIUM(VI) HIGH RANGE
8.21. CHROMIUM(VI) HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 1000 µg/L (as Cr(VI))
1 µg/L
±5 µg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D1687-92, Diphenylcarbohydrazide method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93723-0
Chromium(VI) High Range Reagent
1 packet
REAGENT SETS
HI93723-01
Reagents for 100 tests
HI93723-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Chromium(VI) HR method using the procedure described
in the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93723-0 Chromium(VI) High Range
Reagent. Replace the cap and shake vigorously for about
10 seconds.
88
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in µg/L of chromium (Cr6+).
CHROMIUM(VI) HIGH RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of chromate (CrO42¯) and dichromate
(Cr2O72¯).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Vanadium above 1 ppm. However, waiting 10 minutes before reading removes the interference.
Iron above 1 ppm.
Mercurous and mercuric ions cause slight inhibition of the reaction.
89
CHEMICAL OXYGEN DEMAND LOW RANGE (16 mm VIAL)
8.22. CHEMICAL OXYGEN DEMAND LOW RANGE (16 mm VIAL)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 150 mg/L (as O2)
1 mg/L
±5 mg/L or ±4% of reading @ 25 °C, whichever is greater
LED with narrow band interference filter @ 420 nm
Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93754A-0*
COD Low Range Reagent Vial
2 vials
DEIONIZED120
Deionized Water
2 mL
*Reagent Vial identification: COD A, red label.
REAGENT SETS
Reagents for 24 tests
HI93754A-25
For other accessories see page 267.
Note: Store the unused vials in a cool and dark place.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions, and notes. Failure
to do so may result in serious injury to the operator.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial
may be used more than once. The blank vial is stable for several months at room temperature.
For improved accuracy, run a blank for each set of measurements and always use the same lot of
reagents for blank and samples.
• Choose a homogeneous sample. Samples containing solids capable of settling need to be
homogenized with a blender.
• Preheat the HANNA® Reactor HI839800 to 150 °C (302 °F).The optional HI740217 safety
shield is strongly recommended.
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly
explosive atmosphere.
90
• Add 2.0 mL of deionized water to the first vial (#1) and 2.0 mL of sample to the second vial
(#2), while keeping the vials at a 45-degree angle. Replace the cap and invert several times to
mix.
WARNING: The vials will become hot during mixing, use caution when handling.
• Insert the vials into the reactor and heat them for 2 hours at 150 °C.
• At the end of the digestion period switch off the reactor. Wait for
twenty minutes to allow the vials to cool to about 120 °C.
CHEMICAL OXYGEN DEMAND LOW RANGE (16 mm VIAL)
• Remove the cap from two HI93754A-0 COD Low Range Reagent Vials.
• Invert each vial several times while still warm, then place them in
the test tube rack.
WARNING: The vials are still hot, use caution when handling.
• Leave the vials in the tube rack to cool to room temperature. Do not
shake or invert them, the samples may become turbid.
91
CHEMICAL OXYGEN DEMAND LOW RANGE (16 mm VIAL)
• Select COD LR (16) method using the procedure described in the Method Selection section (see
page 19).
• Insert the 16 mm vial adapter using the procedure described in the
Using the 16 mm Vial Adapter section (see page 22).
• Place the blank vial (#1) into the holder.
• Press the Zero key. The display will show -0.0- when the meter is zeroed and ready for
measurement.
• Remove the vial.
#2
• Place the sample vial (#2) into the holder.
• Press Read to start the reading. The instrument displays the results in mg/L of oxygen (O2).
INTERFERENCES
Interference may be caused by:
Chloride (Cl¯) above 2000 mg/L.
Samples with higher chloride concentration should be diluted.
92
#1
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 1500 mg/L (as O2)
1 mg/L
±15 mg/L or ±4% of reading @ 25 °C, whichever is greater
LED with narrow band interference filter @ 610 nm
Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93754B-0*
COD Medium Range Reagent Vial
2 vials
DEIONIZED120
Deionized Water
2 mL
*Reagent Vial identification: COD B, white label.
REAGENT SETS
Reagents for 24 tests
HI93754B-25
For other accessories see page 267.
Note: Store the unused vials in a cool and dark place.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions, and notes. Failure
to do so may result in serious injury to the operator.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial
may be used more than once. The blank vial is stable for several months at room temperature. For
improved accuracy measurement, run a blank for each set of measurements and always use the
same lot of reagents for blank and samples.
CHEMICAL OXYGEN DEMAND MEDIUM RANGE (16 mm VIAL)
8.23. CHEMICAL OXYGEN DEMAND MEDIUM RANGE (16 mm VIAL)
• Choose a homogeneous sample. Samples containing solids capable of settling need to be
homogenized with a blender.
• Preheat the HANNA® Reactor HI839800 to 150 °C (302 °F). Use of the optional HI740217
safety shield is strongly recommended.
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly
explosive atmosphere.
93
CHEMICAL OXYGEN DEMAND MEDIUM RANGE (16 mm VIAL)
• Remove the cap from two HI93754B-0 COD Medium Range Reagent Vials.
• Add 2.0 mL of deionized water to the first vial (#1) and 2.0 mL of sample to the second vial
(#2), while keeping the vials at a 45-degree angle. Replace the cap and invert several times to
mix.
WARNING: The vials will become hot during mixing, use caution when handling
• Insert the vials into the reactor and heat them for 2 hours at 150 °C.
• At the end of the digestion period switch off the reactor. Wait for
twenty minutes to allow the vials to cool to about 120 °C.
• Invert each vial several times while still warm, then place them in
the test tube rack.
WARNING: The vials are still hot, use caution when handling.
• Leave the vials in the tube rack to cool to room temperature. Do not
shake or invert them, the samples may become turbid.
94
• Insert the 16 mm vial adapter using the procedure
described in the Using the 16 mm Vial Adapter section
(see page 22).
#1
• Place the blank vial into the holder.
• Press Zero key the display will show -0.0- when the meter is zeroed and ready for
measurement.
• Remove the vial.
#2
• Place the sample vial (#2) into the holder.
• Press Read to start the reading. The instrument displays the results in mg/L of oxygen
(O2).
CHEMICAL OXYGEN DEMAND MEDIUM RANGE (16 mm VIAL)
• Select COD MR (16) method using the procedure described in the Method Selection section
(see page 19).
INTERFERENCES
Interference may be caused by:
Chloride (Cl¯) above 2000 mg/L.
Samples with higher chloride concentration should be diluted.
95
CHEMICAL OXYGEN DEMAND HIGH RANGE (16 mm VIAL)
8.24. CHEMICAL OXYGEN DEMAND HIGH RANGE (16 mm VIAL)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 15000 mg/L (as O2)
1 mg/L
±150 mg/L or ±2% of reading @ 25 °C, whichever is greater
LED with narrow band interference filter @ 610 nm
Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93754C-0*
COD High Range Reagent Vial 2 vials
DEIONIZED120
Deionized Water
0.2 mL
* Reagent Vial identification: COD C, green label
REAGENT SETS
Reagents for 24 tests
HI93754C-25
For other accessories see page 267.
Note: Store the unused vials in their container in a cool and dark place.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions and notes. Failure
to do so may result in serious injury to the operator.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial
may be used more than once. The blank vial is stable for several months at room temperature. For
improved accuracy measurement, run a blank for each set of measurements and always use the
same lot of reagents for blank and samples.
• Choose a homogeneous sample. Samples containing solids capable of settling need to be
homogenized with a blender.
• Preheat the HANNA® Reactor HI839800 to 150 °C (302 °F). Use of the optional HI740217
safety shield is strongly recommended.
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly
explosive atmosphere.
96
• Add 0.2 mL of deionized water to the first vial (#1) and 0.2 mL of sample to the second vial
(#2), while keeping the vials at a 45-degree angle. Replace the cap and invert several times to
mix.
WARNING: The vials will become hot during mixing, use caution when handling.
• Insert the vials into the reactor and heat them for 2 hours at 150 °C.
• At the end of the digestion period switch off the reactor. Wait for
twenty minutes to allow the vials to cool to about 120 °C.
CHEMICAL OXYGEN DEMAND HIGH RANGE (16 mm VIAL)
• Remove the cap from two HI93754C-0 COD High Range Reagent Vials.
• Invert each vial several times while still warm, then place them in
the test tube rack.
WARNING: The vials are still hot, use caution when handling.
• Leave the vials in the tube rack to cool to room temperature. Do not
shake or invert them, the samples may become turbid.
97
CHEMICAL OXYGEN DEMAND HIGH RANGE (16 mm VIAL)
• Select COD HR (16) method using the procedure described in the Method Selection section
(see page 19).
• Insert the 16 mm vial adapter using the procedure described in
the Using the 16 mm Vial Adapter section (see page 22).
• Place the blank vial (#1) into the holder.
• Press Zero key. The display will show -0.0- when the meter is zeroed and ready for
measurement.
#2
• Remove the vial.
• Place the sample vial (#2) into the holder.
• Press Read key to start the reading. The instrument displays the results in mg/L of oxygen
(O2).
INTERFERENCES
Interference may be caused by:
Chloride (Cl¯) above 20000 mg/L.
Samples with higher chloride concentration should be diluted.
98
#1
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 500 PCU (Platinum Cobalt Units)
1 PCU
±10 PCU ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, Colorimetric Platinum Cobalt method
COLOR OF WATER
8.25. COLOR OF WATER
REQUIRED ACCESSORIES
0.45 µm membrane for true color measurement.
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Color of Water method using the procedure described in
the Method Selection section (see page 19).
10 mL
• Fill the first cuvette (#1) up to the mark with deionized water and
replace the cap.
#1
#1
• Place the blank (#1) into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette
99
COLOR OF WATER
• Fill the second cuvette (#2) up to the mark with unfiltered
sample and replace the cap. This is the apparent color.
10 mL
#2
• Filter 10 mL of sample through a filter with a 0.45 µm
membrane into the third cuvette (#3), up to the 10 mL
mark and replace the cap. This is the true color.
• Insert the apparent color cuvette (#2) into the instrument
and close the lid.
#3
#2
• Press Read to start the reading. The meter displays the value of apparent color in Platinum
Cobalt Units (PCU).
• Remove the apparent color cuvette(#2) from the
instrument, insert the true color cuvette (#3) into the
instrument and close the lid.
#3
•Press Read to start the reading. The meter displays the true color in Platinum Cobalt Units
(PCU).
100
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
COPPER LOW RANGE
8.26. COPPER LOW RANGE
0.000 to 1.500 mg/L (as Cu2+)
0.001 mg/L
±0.010 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the EPA method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI95747-0
Copper Low Range Reagent
1 packet
REAGENT SETS
HI95747-01
Reagents for 100 tests
HI95747-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Copper LR method using the procedure described in the
Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
101
COPPER LOW RANGE
• Remove the cuvette.
• Add one packet of HI95747-0 Copper Low Range Reagent.
Replace the cap and shake gently for about 15 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the results in mg/L of copper (Cu).
INTERFERENCES
Interference may be caused by:
Silver, Cyanide.
For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted
between 6 and 8.
102
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
COPPER HIGH RANGE
8.27. COPPER HIGH RANGE
0.00 to 5.00 mg/L (as Cu2+)
0.01 mg/L
±0.02 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the EPA method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93702-0
Copper High Range Reagent
1 packet
REAGENT SETS
HI93702-01
Reagents for 100 tests
HI93702-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Copper HR method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
103
COPPER HIGH RANGE
• Remove the cuvette.
• Add one packet of HI93702-0 Copper High Range Reagent.
Replace the cap and shake gently for about 15 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the results in mg/L of copper (Cu).
INTERFERENCES
Interference may be caused by:
Silver, Cyanide.
For samples overcoming buffering capacity of reagent (around pH 6.8), pH should be adjusted
between 6 and 8.
104
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 80 mg/L (as CYA)
1 mg/L
±1 mg/L ±15% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the turbidimetric method
CYANURIC ACID
8.28. CYANURIC ACID
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93722-0
Cyanuric Acid Reagent
1 packet
REAGENT SETS
HI93722-01
Reagents for 100 tests
HI93722-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Cyanuric Acid method using the procedure described in the
Method Selection section (see page 19).
• Fill the first cuvette with 10 mL of unreacted
sample (up to the mark) and replace the cap.
#1
10 mL
#1
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
105
CYANURIC ACID
• Fill a beaker with 25 mL sample (up to the mark).
• Add one packet of HI93722-0 Cyanuric Acid Reagent and
mix to dissolve.
#2
• Fill a second cuvette with 10 mL of the reacted sample,
up to the mark, and replace the cap.
• Reinsert the cuvette into the instrument and close the lid.
10 mL
#2
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 45 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the concentration in mg/L of cyanuric acid.
106
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.00 mg/L (as F-)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, SPADNS method
REQUIRED REAGENT
CodeDescriptionQuantity
HI93729-0
Fluoride Low Range Reagent
4 mL
FLUORIDE LOW RANGE
8.29. FLUORIDE LOW RANGE
REAGENT SETS
HI93729-01
Reagents for 100 tests
HI93729-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Fluoride LR method using the procedure
described in the Method Selection section (see page 19).
• Add 2 mL of HI93729-0 Fluoride Low Range Reagent to
two cuvettes.
• Use a plastic pipette to fill the cuvette to the 10 mL
mark with deionized water (#1), replace the cap and
invert several times to mix.
• Use a plastic pipette to fill the second cuvette to
the 10 mL mark with unreacted sample (#2),
replace the cap and invert several times to mix.
2 mL
10 mL
10 mL
#1
#2
#1
• Place the first cuvette (#1) into the holder and close the lid.
107
FLUORIDE LOW RANGE
• Press Timer and the display will show the countdown prior to zeroing the blank or, alternatively,
wait for two minutes and press Zero. The display will show “-0.0-” when the meter is zeroed
and ready for measurement.
#2
• Remove the cuvette.
• Insert the second cuvette (#2) with the reacted sample into the
instrument and close the lid.
• Press Read to start reading. The instrument displays the results in mg/L of fluoride (F-).
Note: For wastewater or seawater samples, before performing measurements, distillation
is required. For most accurate results use two graduated pipettes to deliver exactly 8 mL of
deionized water and 8 mL of sample.
INTERFERENCES
Interferences may be caused by:
Alkalinity (as CaCO3) above 5000 mg/L
Aluminum above 0.1 mg/L
Iron, ferric above 10 mg/L
Chloride above 700 mg/L
ortho-Phosphate above 16 mg/L
Sodium hexametaphosphate above 1.0 mg/L
Sulfate above 200 mg/L
Highly colored and turbid samples may require distillation
Highly alkaline samples can be neutralized with nitric acid.
108
SPECIFICATIONS
Range
0.0 to 20.0 mg/L (as F-)
Resolution
0.1 mg/L
Accuracy
±0.5 mg/L ±3% of reading at 25 °C
Light Source
LED with narrow band interference filter @ 575 nm
Method
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, SPADNS method
REQUIRED REAGENT
CodeDescriptionQuantity
HI93739A-0
Fluoride High Range Reagent A
2 mL
HI93739B-0
Fluoride High Range Reagent B
8 mL
FLUORIDE HIGH RANGE
8.30. FLUORIDE HIGH RANGE
REAGENT SETS
Reagents for 100 tests
HI93739-01
HI93739-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Fluoride HR method using the procedure
described in the Method Selection section
(see page 19).
• Add 2.00 mL of HI93739A-0 Fluoride High Range
Reagent A to the cuvette and use the pipette to fill
up the cuvette to the 10 mL mark with HI93739B-0
Fluoride High Range Reagent B.
• Replace the cap and invert several times to mix.
• Place the cuvette into the holder and close the lid.
109
FLUORIDE HIGH RANGE
• Press Timer and the display will show the countdown prior to zeroing the blank or, alternatively,
wait for one minute and press Zero. The display will show “-0.0-” when the meter is zeroed
and ready for measurement.
• Remove the cuvette.
• Add 1 mL of sample to the cuvette using the second 1 mL syringe.
• Replace the cap and invert several times to mix.
• Insert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for one minute and press Read. When the timer ends the meter will perform the reading.
The instrument displays the results in mg/L of fluoride (F-).
110
INTERFERENCES
Interferences may be caused by:
Alkalinity (as CaCO3) above 5000 mg/L
Aluminum above 0.1 mg/L
Iron, ferric above 10 mg/L
Chloride above 700 mg/L
ortho-Phosphate above 16 mg/L
Sodium hexametaphosphate above 1.0 mg/L
Sulfate above 200 mg/L
Highly colored and turbid samples may require distillation
Highly alkaline samples can be neutralized with nitric acid.
FLUORIDE HIGH RANGE
Note: For wastewater or seawater samples, before performing measurements, distillation is
required.
111
HARDNESS, CALCIUM
8.31. HARDNESS, CALCIUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.70 mg/L (as CaCO3)
0.01 mg/L
±0.11 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, Calmagite method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93720A-0
Calcium Hardness Reagent A
0.5 mL
HI93720B-0
Calcium Hardness Reagent B
0.5 mL
HI93720C-0
Calcium Hardness Reagent C
1 drop
REAGENT SETS
Reagents for 100 tests
HI93720-01
HI93720-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Hardness (Calcium) method using the procedure
described in the Method Selection section (see page 19).
• Rinse a graduated beaker several times with unreacted
sample, before filling it to the 50 mL mark with the sample.
• Add 0.5 mL of HI93720A-0 Calcium Hardness Reagent A
and swirl to mix.
• Add 0.5 mL of HI93720B-0 Calcium Hardness
Reagent B and swirl to mix. Use this solution to rinse
2 cuvettes before filling them up to the 10 mL mark.
112
#1
#2
• Replace the cap and invert the cuvette several times to mix.
This is the blank.
HARDNESS, CALCIUM
• Add 1 drop of HI93720C-0 Calcium Hardness Reagent C to one cuvette (#1).
#1
• Place the blank (#1) into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
#2
• Remove the blank (#1) and insert the second cuvette (#2)
into the instrument.
• Press Read to start the reading. The instrument displays concentration in mg/L of calcium
carbonate (CaCO3).
• Press  or  to access the second level functions.
113
HARDNESS, CALCIUM
• Press the Chem Frm key to convert the results to French degrees (°f), German degrees
(°dH), and English degrees (°E).
• Press  or  to return to the measurement screen.
Note: This test will detect any calcium contamination in the beaker, measuring syringes or
sample cells. To test cleanliness repeat the test multiple times until you obtain consistent
results.
SAMPLE DILUTION
This meter is designed to determine low levels of hardness, typically found in water purification
systems.
When testing some other sources of water, it is not uncommon to come across levels of hardness
that are greater than the range of this meter.
This problem can be overcome through dilution. Dilutions must be performed with hardness-free
water or the readings will be erroneous.
A dilution to reduce the level of hardness by a factor of one hundred is performed as follows:
• Fill a 1 mL syringe with the sample.
• Place the syringe in a 50 mL beaker, making sure that the beaker is clean and empty, and
inject 0.5 mL into the beaker.
• Fill the beaker up to the 50 mL mark with hardness-free water.
INTERFERENCES
Interference may be caused by excessive amounts of heavy metals.
114
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.00 mg/L (CaCO3)
0.01 mg/L
±0.11 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, EDTA Colorimetric method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93719A-0
Magnesium Hardness Reagent A
0.5 mL
HI93719B-0
Magnesium Hardness Reagent B
0.5 mL
HI93719C-0
Magnesium Hardness Reagent C
1 drop
HI93719D-0
Magnesium Hardness Reagent D
1 drop
HARDNESS, MAGNESIUM
8.32. HARDNESS, MAGNESIUM
REAGENT SETS
HI93719-01
Reagents for 100 tests
HI93719-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Hardness (Magnesium) method using the procedure
described in the Method Selection section (see page 19).
• Rinse a graduated beaker several times with unreacted sample
before filling it to the 50 mL mark with the sample.
• Add 0.5 mL of HI93719A-0 Magnesium Hardness Reagent A, then
swirl to mix.
• Add 0.5 mL of HI93719B-0 Magnesium Hardness Reagent B and
swirl to mix. Use this solution to rinse 2 cuvettes.
115
HARDNESS, MAGNESIUM
• Fill both cuvettes up to the 10 mL mark.
10 mL
10 mL
#1
#2
• Add 1 drop of HI93719C-0 Magnesium Hardness
Reagent C to one cuvette (#1), replace the cap and
invert the cuvette several times to mix. This is the
blank.
• Add 1 drop of HI93719D-0 Magnesium Hardness
Reagent D to the second cuvette (#2), replace the cap
and invert the cuvette several times to mix. This is the
sample.
#1
#2
#1
• Place the blank (#1) into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
#2
• Remove the blank (#1), insert the sample (#2) into the
instrument and close the lid.
116
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the results to French degrees (°f), German degrees
(°dH), and English degrees (°E).
HARDNESS, MAGNESIUM
• Press Read to start the reading. The instrument displays concentration in mg/L of calcium
carbonate. (CaCO3).
• Press  or  to return to the measurement screen.
Note: This test will detect any magnesium contamination in the beakers, measuring syringes,
or sample cells. To test cleanliness repeat the test multiple times until you obtain consistent
results.
SAMPLE DILUTION
This meter is designed to determine hardness typically found in water purification systems. In order
to measure samples with high hardness, follow dilution procedure explained on page 110.
INTERFERENCES
Interference may be caused by excessive amounts of heavy metals.
117
HARDNESS, TOTAL LOW RANGE
8.33. HARDNESS, TOTAL LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 250 mg/L (as CaCO3)
1 mg/L
±5 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the EPA recommended method 130.1
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93735IND-0
Hardness Indicator Reagent
0.5 mL
HI93735A-LR
Hardness Low Range Reagent A
9 mL
HI93735B-0
Hardness Buffer Reagent B
2 drops
HI93735C-0
Fixing Reagent
1 packet
REAGENT SETS
Reagents for 100 tests (LR, 0 to 250 mg/L)
HI93735-00
HI93735-0
Reagents for 300 tests (LR - 100 tests, MR - 100 tests, HR - 100 tests)
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Hardness Total LR method using the
procedure described in the Method Selection section
(see page 19).
• Add 0.5 mL of unreacted sample to the cuvette. Add
0.5 mL of HI93735IND-0 Hardness Indicator Reagent.
• With the plastic dropper fill the cuvette up to the
10 mL mark with HI93735A-LR Hardness Low
Range Reagent A.
• Add two drops of HI93735B-0 Hardness Buffer
Reagent B. Replace the cap and invert 5 times to
mix.
118
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
HARDNESS, TOTAL LOW RANGE
• Place the cuvette into the holder and close the lid.
• Remove the cuvette and add the contents of one
packet of HI93735C-0 Fixing Reagent. Replace
the cap and shake gently to mix 20 seconds.
• Place the cuvette into the holder and close the lid.
• Press Read to start the reading. The instrument displays concentration in mg/L of calcium
carbonate (CaCO3).
• Press  or  to access the second level functions.
119
HARDNESS, TOTAL LOW RANGE
• Press the Chem Frm key to convert the result to French degrees (°f), English degrees (°E),
and German degrees (°dH).
• Press  or  to return to the measurement screen.
INTERFERENCE
Interferences may be caused by excessive amounts of heavy metals.
120
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
200 to 500 mg/L (as CaCO3)
1 mg/L
±7 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the EPA recommended method 130.1
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93735IND-0
Hardness Indicator Reagent
0.5 mL
HI93735A-MR
Hardness Medium Reagent A
9 mL
HI93735B-0
Hardness Buffer Reagent B
2 drops
HI93735C-0
Fixing Reagent
1 packet
REAGENT SETS
Reagents for 100 tests (MR, 200 to 500 mg/L)
HI93735-01
HI93735-0
Reagents for 300 tests (LR - 100 tests, MR - 100 tests, HR - 100 tests)
For other accessories see page 267.
HARDNESS, TOTAL MEDIUM RANGE
8.34. HARDNESS, TOTAL MEDIUM RANGE
MEASUREMENT PROCEDURE
• Select the Hardness Total MR method using the procedure
described in the Method Selection section (see page 19).
• Add 0.5 mL of unreacted sample to the cuvette.
Add 0.5 mL of HI93735IND-0 Hardness
Indicator Reagent.
• With the plastic dropper fill the cuvette up to the
10 mL mark with HI93735A-MR Hardness Medium Range
Reagent A.
• Add two drops of HI93735B-0 Hardness Buffer
Reagent B. Replace the cap and invert 5 times to mix.
121
HARDNESS, TOTAL MEDIUM RANGE
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette and add one packet of HI93735C-0
Fixing Reagent. Replace the cap and shake gently to mix
20 seconds.
• Place the cuvette into the holder and close the lid.
• Press Read to start the reading. The instrument displays concentration in mg/L of calcium
carbonate (CaCO3).
• Press  or  to access the second level functions.
122
• Press  or  to return to the measurement screen.
INTERFERENCES
Interferences may be caused by excessive amounts of heavy metals.
HARDNESS, TOTAL MEDIUM RANGE
• Press the Chem Frm key to convert the result to French degrees (°f), English degrees (°E)
and German degrees (°dH).
123
HARDNESS, TOTAL HIGH RANGE
8.35. HARDNESS, TOTAL HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
400 to 750 mg/L (as CaCO3)
1 mg/L
±10 mg/L ±2% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the EPA recommended method 130.1
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93735IND-0
Hardness Indicator Reagent
0.5 mL
HI93735A-HR
Hardness High Range Reagent A
9 mL
HI93735B-0
Hardness Buffer Reagent B
2 drops
HI93735C-0
Fixing Reagent
1 packet
REAGENT SETS
Reagents for 100 tests (HR, 400 to 750 mg/L)
HI93735-02
HI93735-0
Reagents for 300 tests (LR - 100 tests, MR - 100 tests, HR - 100 tests)
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Total Hardness HR method using the procedure
described in the Method Selection section (see page 19).
• Add 0.5 mL of unreacted sample to the cuvette. Add
0.5 mL of HI93735IND-0 Hardness Indicator Reagent.
• With the plastic dropper fill the cuvette up to the 10 mL
mark with HI93735A-HR Hardness High Range Reagent A.
• Add two drops of HI93735B-0 Hardness Buffer
Reagent B. Replace the cap and invert 5 times to mix.
124
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
HARDNESS, TOTAL HIGH RANGE
• Place the cuvette into the holder and close the lid.
• Remove the cuvette and add one packet of HI93735C-0
Fixing Reagent. Replace the cap and shake gently to mix
20 seconds.
• Place the cuvette into the holder and close the lid.
• Press Read to start the reading. The instrument displays concentration in mg/L of calcium
carbonate (CaCO3).
• Press  or  to access the second level functions.
125
HARDNESS, TOTAL HIGH RANGE
• Press the Chem Frm key to convert the result to French degrees (°f), English degrees (°E)
and German degrees (°dH).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interferences may be caused by excessive amounts of heavy metals
126
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method 0 to 400 µg/L (as N2H4)
1 µg/L
±4% of full scale reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
method D1385, p-Dimethylaminobenzaldehyde method
REQUIRED REAGENT
Code
Description
HI93704-0
Hydrazine Reagent
HYDRAZINE
8.36. HYDRAZINE
Quantity
24 drops
REAGENT SETS
HI93704-01
Reagents for 100 tests
HI93704-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Hydrazine method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill cuvette (#1) with 10 mL of deionized water (up to the mark).
#1
• Fill a second cuvette (#2) with 10 mL of unreacted sample
(up to the mark).
• Add 12 drops of the HI93704-0 reagent
to each cuvette. Replace the caps and
shake gently to mix (about 30 seconds).
#1
10 mL
#2
#2
127
HYDRAZINE
• Place the cuvette (#1) into the holder and close the lid.
#1
• Press Timer and the display will show the countdown prior to zeroing the blank or, alternatively
wait for 12 minutes and press Zero. The display will show “-0.0-” when the meter is zeroed
and ready for measurement.
• Remove the blank.
• Insert the cuvette with the reacted sample (#2) into the instrument
and close the lid.
#2
• Press Read to start the reading. The instrument displays concentration in µg/L of hydrazine (N2H4).
INTERFERENCES
Interference may be caused by: highly colored samples, highly turbid samples, aromatic amines.
128
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 12.5 mg/L (as I2)
0.1 mg/L
±0.1 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, DPD method
REQUIRED REAGENTS
Code
Description
HI93718-0
Iodine Reagent
IODINE
8.37. IODINE
Quantity
1 packet
REAGENT SETS
HI93718-01
Reagents for 100 tests
HI93718-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Iodine method using the procedure described in the
Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
#1
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
129
IODINE
• Remove the cap and add one packet of HI93718-0 Iodine
Reagent. Replace the cap and shake gently for about
20 seconds to dissolve most of the reagent.
• Reinsert the cuvette into the instrument and close the lid.
#1
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the concentration in mg/L of Iodine (I2).
INTERFERENCES
Interference may be caused by: Bromine, Chlorine, Ozone, Oxidized forms of Chromium and
Manganese.
In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately
2 minutes after adding the reagent.
In case of water with alkalinity greater than 250 mg/L CaCO3 or acidity greater than 150 mg/L
CaCO3, the color of the sample may develop only partially, or may rapidly fade. To resolve this,
neutralize the sample with diluted HCl or NaOH.
130
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
IRON LOW RANGE
8.38. IRON LOW RANGE
0.000 to 1.600 mg/L (as Fe)
0.001 mg/L
±0.010 mg/L ±8% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the TPTZ Method.
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93746-0
Iron Low Range Reagent
2 packets
REAGENT SETS
HI93746-01
Reagents for 50 tests
HI93746-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Iron LR method using the procedure described in
the Method Selection section (see page 19).
25 mL
• Fill one graduated mixing cylinder up to the 25 mL mark
with deionized water.
• Add one packet of HI93746-0 Iron Low Range Reagent,
close the cylinder and shake vigorously for 30 seconds.
This is the blank.
• Fill a cuvette with 10 mL of the blank (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
131
IRON LOW RANGE
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Fill another graduated mixing cylinder up to the 25 mL
mark with the sample.
25 mL
• Add one packet of HI93746-0 Iron Low Range Reagent,
close the cylinder and shake vigorously for 30 seconds.
This is the reacted sample.
• Fill a cuvette with 10 mL of the reacted sample (up to the
mark) and replace the cap.
• Insert the sample into the instrument and close the lid.
132
10 mL
IRON LOW RANGE
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 30 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays concentration in mg/L of iron (Fe).
INTERFERENCES
Interference may be caused by:
Cadmium above 4.0 mg/L
Chromium3+ above 0.25 mg/L
Chromium6+ above 1.2 mg/L
Cobalt above 0.05 mg/L
Copper above 0.6 mg/L
Cyanide above 2.8 mg/L
Manganese above 50.0 mg/L
Mercury above 0.4 mg/L
Molybdenum above 4.0 mg/L
Nickel above 1.0 mg/L
Nitrite ion above 0.8 mg/L
Sample pH should be between 3 and 4 to avoid fading of the developed to fade or turbidity
formation.
133
IRON HIGH RANGE
8.39. IRON HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 5.00 mg/L (as Fe)
0.01 mg/L
±0.04 mg/L ±2% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 23rd Edition, 3500-Fe B., Phenanthroline Method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93721-0
Iron High Range Reagent
1 packet
REAGENT SETS
HI93721-01
Reagents for 100 tests
HI93721-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Iron HR method using the procedure described in
the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” the meter is zeroed and ready for measurement.
134
• Reinsert the cuvette into the instrument and close the lid.
IRON HIGH RANGE
• Remove the cuvette and add the content of one packet of
HI93721-0 Iron High Range Reagent. Replace the cap and
shake until powder is completely dissolved.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and press Read. When the timer ends the meter will perform the reading.
The instrument displays the result in mg/L of iron (Fe).
INTERFERENCES
Interference may be caused by:
Molybdate Molybdenum above 50 ppm
Calcium above 10000 ppm (as CaCO3)
Magnesium above 100000 ppm (as CaCO3)
Chloride above 185000 ppm.
135
IRON(II)
8.40. IRON(II)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 6.00 mg/L Fe2+
0.01 mg/L
±0.10 mg/L ±2% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 23rd Edition, 3500-Fe B., Phenanthroline Method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96776-0
Iron(II) Reagent
1 packet
REAGENTS SETS
HI96776-01
Reagents Set, 100 Tests
HI96776-03
Reagents Set, 300 Tests
For other accessories see page 267.
PRINCIPLE
In aqueous solution, reactive ferrous iron (Fe2+) reacts with 1,10-phenanthroline to form an
orange-red complex.
APPLICATION
Surface water, drinking water, mineral and groundwater, process control
SIGNIFICANCE AND USE
Surface water typically contains up to 0.7 mg/L or iron. Drinking water typically contains up to 0.3 mg/L
or iron, but this level may increase significantly if plumbing fixtures contain iron. In well-oxygenated,
non-acidic waters, iron exists mainly in the ferric form (Fe3+) and will precipitate as iron oxide
hydroxide (FeO(OH)). However, anoxic water may have high levels of dissolved ferrous iron (Fe2+)
which could precipitate in heating/cooling systems or other equipment after exposure to air.
The Iron(II) method measures the ferrous (Fe2+) form of iron.
136
WARNING: Method is temperature-dependent. Sample temperature must be 18 °C - 22 °C.
• Select the Iron(II) method using the procedure described in the Method
Selection section (see page 19)
IRON(II)
MEASUREMENT PROCEDURE
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid
• P ress the Zero key. The display will show "-0.0-"; the meter is zeroed and ready for measurement.
137
IRON(II)
• Remove the cuvette and add the content of one packet of
HI96776-0 Iron(II) Reagent. Replace the cap and shake
gently for 30 seconds.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait
for 3 minutes and press Read. The instrument displays the result in mg/L of Iron (Fe2+).
WARNING: Timing is critical for accurate measurement. Reaction times beyond 3 minutes may cause
some ferric iron (Fe3+) to also react, producing false high measurements.
138
Interference may be caused by:
Extreme pH or highly buffered samples. The pH of the sample must be 3.8 - 5.5 after addition of
the reagent.
Ammonium above 500 mg/L
Calcium above 500 mg/L
Carbonate above 50 mg/L
Chloride above 1000 mg/L
Chromium(III) and (VI) above 50 mg/L
Cobalt above 50 mg/L
Copper above 10 mg/L
Lead above 50 mg/L
Mercury above 50 mg/L
Nickel above 25 mg/L
Nitrate above 50 mg/L
Potassium above 500 mg/L
Sodium above 500 mg/L
Silver above 100 mg/L
Sulfate above 1000 mg/L
Tin above 5 mg/L
Zinc above 50 mg/L
IRON(II)
INTERFERENCES
139
IRON(II)/(III)
8.41. IRON(II)/(III)
SPECIFICATIONS
Range
0.00 to 6.00 mg/L Fe
Resolution
0.01 mg/L
Accuracy
±0.10 mg/L ±2% of reading at 25 °C
Light Source
LED with narrow band interference filter @ 525 nm
MethodAdaptation of Standard Methods for the Examination of Water and Wastewater, 23rd Edition, 3500-Fe B., Phenanthroline Method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96777A-0
Iron(II)/(III) Reagent A
1 packet
HI96777B-0
Iron(II)/(III) Reagent B
1 packet
REAGENTS SETS
HI96777-01
Reagent Set, 100 Tests
HI96777-03
Reagent Set, 300 Tests
For other accessories see page 267.
PRINCIPLE
During the first measurement, ferrous iron (Fe2+) reacts with 1,10-phenanthroline to form an
orange-red complex. During the second measurement, ferric iron (Fe3+) is converted to ferrous iron
(Fe2+) by the addition of Reagent B; the resulting measurement is the sum of ferrous (Fe2+) and
ferric (Fe3+) iron.
APPLICATION
Surface water, drinking water, mineral and groundwater, process control.
SIGNIFICANCE AND USE
Surface water typically contains up to 0.7 mg/L of iron. Drinking water typically contains up to 0.3 mg/L of
iron, but this level may increase significantly if plumbing fixtures contain iron. In well-oxygenated,
non-acidic waters, iron exists mainly in the ferric form (Fe3+) and will precipitate as iron oxide
hydroxide (FeO(OH)). However, anoxic water may have high levels of dissolved ferrous iron (Fe2+)
which could precipitate in heating/cooling systems or other equipment after exposure to air.
The Iron(II)/(III) method can be used to distinguish between the ferrous (Fe2+) and ferric (Fe3+)
forms of iron in a 2-step measurement process.
140
IRON(II)/(III)
MEASUREMENT PROCEDURE
• Select the Iron(II)/(III) method using the procedure described in the Method
Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show "-0.0-"; the meter is zeroed and ready for
measurement.
• Remove the cuvette and add the content of one packet of
HI96777A-0 Iron(II)/(III) Reagent A. Replace the cap and
shake gently for 30 seconds.
• Reinsert the cuvette into the instrument and close the lid.
141
IRON(II)/(III)
• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait
for 3 minutes and press Read 1. The instrument displays the result in mg/L of Iron (Fe2+).
WARNING: Timing is critical for accurate measurement.
Reaction times beyond 3 minutes may cause some ferric iron (Fe3+) to react prematurely, producing
high Fe2+/low Fe3+ measurements.
• Remove the cap from the cuvette and add the content of one
packet of HI96777B-0 Iron(II)/(III) Reagent B. Replace the cap
shake gently for 30 seconds.
• Reinsert the cuvette into the instrument and close the lid.
Note: If pressing the Zero key, the instrument will return to measure
Iron(II) (Fe2+).
• Press Timer and the display will show the countdown prior to the measurement
or, alternatively, wait for 3 minutes and press Read 2. The instrument displays the result in mg/L of
Iron(III) (Fe3+).
142
IRON(II)/(III)
• Press Chem Frm to cycle through the available chemical forms of Fe2+,Fe3+ and (Fe2+ +Fe3+).
• Press LOG to store the measurement. Each chemical form may be logged independently.
INTERFERENCES
Interference may be caused by:
Extreme pH or highly buffered samples. The pH of the sample must be 3.8-5.5 after addition of the reagents.
Ammonium above 500 mg/L
Calcium above 500 mg/L
Carbonate above 50 mg/L
Chloride above 1000 mg/L
Chromium(III) and (VI) above 50 mg/L
Cobalt above 50 mg/L
Copper above 10 mg/L
Lead above 50 mg/L
Mercury above 50 mg/L
Nickel above 25 mg/L
Nitrate above 50 mg/L
Potassium above 500 mg/L
Sodium above 500 mg/L
Silver above 100 mg/L
Sulfate above 1000 mg/L
Tin above 5 mg/L
Zinc above 50 mg/L
143
IRON, TOTAL (16 mm VIAL)
8.42. IRON, TOTAL (16 mm VIAL)
SPECIFICATIONS
Range
0.00 to 7.00 mg/L (as Fe)
Resolution
0.01 mg/L
Accuracy
±0.20 mg/L or± 3% of reading, whichever is greater
Light Source
LED with narrow band interference filter @ 525 nm
MethodAdaptation of Standard Methods for the Examination of Water and
Wastewater, 23rd edition, 3500-Fe B., Phenanthroline Method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96778V-0
Total Iron Digestion Vial
1 vial
HI96778A-0
Total Iron Reagent A
1 mL
HI96778B-0
Total Iron Reagent B
1 packet
PERSULFATE/I
Potassium Persulfate Reagent
1 packet
*Reagent vial Identification: IRON, red label
Note: Store the unused vials in their container in a cool and dark place.
REAGENTS SETS
HI96778-25
Reagent Set, 25 Tests.
For other accessories, see page 267.
PRINCIPLE
Digestion of the sample with sulfuric acid and persulfate liberates iron from organic and inorganic
complexes. After digestion, the iron reacts with 1,10-phenanthroline to form an orange-red complex.
APPLICATION: Surface water, drinking water, groundwater, process control, wastewater.
SIGNIFICANCE AND USE
Iron is an abundant, naturally-occurring element found in soils, streams, surface waters, and
groundwater. High levels of iron in drinking water can cause objectionable taste and can stain
plumbing and laundry. Iron in drinking water and wastewater is regulated by the EPA and other
regulatory bodies.
For samples that contain complexed/chelated iron or suspended iron, such as typical wastewater
samples, digestion of the sample is required to allow all of the iron to react with the reagent.
The Total Iron method measures all forms of iron, including ferrous, ferric, dissolved, suspended,
and complexed iron.
144
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and
possibly explosive atmosphere.
IRON, TOTAL (16 mm VIAL)
SAFETY
The acidification of samples containing reactive materials may result in the release of toxic gases,
such as cyanides or sulfides; the preparation of sample and the digestion should be done in a fume
hood. Safety data sheets for all chemical reagents should be read and understood by all personnel
using this method. Specifically, concentrated sulfuric acid is moderately toxic and corrosive to
skin and mucous membranes. Use these reagents in a fume hood whenever possible. If eye or
skin contact occurs, flush with large volumes of water. Always wear skin and eye protection when
working with these reagents.
• Preheat the HANNA® Reactor HI839800 to 150˚C (302˚F). The optional HI740217 safety
shield is strongly recommended.
MEASUREMENT PROCEDURE
• Remove the cap from a HI96778V-0 Digestion Vial
• Add 8.0 mL of sample to the vial, while keeping the vial at a
45-degree angle. Replace the cap and invert several times to mix.
WARNING: The vials will become hot during mixing, use caution when handling.
• Add one packet of PERSULFATE/I Potassium Persulfate Reagent. Replace the cap and shake the
vial vigurously for 60 seconds.
145
IRON, TOTAL (16 mm VIAL)
• Insert the vial into the reactor and heat it for 30 minutes at 150˚C.
• At the end of the digestion switch off the reactor. Allow the vials to cool to room temperature.
Invert each vial several times and place them in the test tube rack.
• Select the Iron (Total) (16) method using the procedure described in the
Method Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in the Using
the 16 mm Vial Adapter section (see page 22).
• Remove the cap from the vial and add exactly 1.0 mL of HI96778A-0
Total Iron Reagent A, while keeping the vial at a 45-degree angle.
• Replace the cap and invert the vial several times to mix.
WARNING: The vials will become hot during mixing, use caution when
handling.
• Wipe the vial thoroughly with HI731318 or a lint-free cloth prior to insertion.
• Place the vial into the holder.
• Press the Zero key. The display will show "-0.0-"; the meter is zeroed and ready for
measurement.
146
• Place the vial into the holder.
IRON, TOTAL (16 mm VIAL)
• Remove the vial from the meter.
• Ensure the temperature of the vial is 18 °C - 22 °C before continuing
to the next step.
• Remove the cap and add one packet of HI96778B-0 Total Iron
Reagent B.
• Replace the cap and shake gently for 30 seconds.
• Wipe the vial thoroughly with HI731318 or a lint-free cloth prior to
insertion.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and press Read. The instrument displays the result in mg/L of Iron, Total (Fe).
INTERFERENCES
Extreme pH or highly buffered samples. After adding the sample to digestion vial, the pH must be less
than 1 for complete destruction of complexes. After addition of HI96778A-0 Total Iron Reagent A, the
pH must be 3.8-5.5.
Interference may be caused by:
Molybdate Molybdenum above 50 ppm
Calcium above 10000 ppm (as CaCO3)
Magnesium above 100000 ppm (as CaCO3)
Chloride above 185000 ppm.
If the sample exhibits turbidity after the digestion, this must be eliminated by filtration.
Matrix factors (e.g. interference ions, color, turbidities, etc.) may have a negative impact on the
measurement and cause false results. Sample with suspended solids cannot be correctly determined
without good homogenization before digestion.
147
MAGNESIUM
8.43. MAGNESIUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 150 mg/L (as Mg2+)
1 mg/L
±5 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the Calmagite method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93752A-Mg
Magnesium Reagent A
1 mL
HI93752B-Mg
Magnesium Reagent B
9 mL
REAGENT SETS
HI937520-01
Reagents for 50 tests
HI937520-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Magnesium method using the procedure
described in the Method Selection section (see page 19).
• Add 1 mL of HI93752A-Mg Magnesium Reagent A to the
cuvette using a 1 mL syringe and use the pipette to fill
the cuvette up to the 10 mL mark with the HI93752B-Mg
Magnesium Reagent B.
• Replace the cap and invert several times to mix.
• Place the cuvette into the holder and close the lid.
148
• Remove the cuvette.
MAGNESIUM
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
0.5 mL
of sample
• Add 0.5 mL of sample to the cuvette using the second 1 mL syringe.
• Replace the cap and invert several times to mix.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 15 seconds and press Read. When the timer ends the meter will perform the reading.
The instrument displays the results in mg/L of magnesium (Mg2+).
INTERFERENCES
Interferences may be caused by: acidity (as CaCO3) above 1000 mg/L, alkalinity (as CaCO3) above
1000 mg/L, Calcium (Ca2+) above 200 mg/L, Iron must be absent, Aluminum must be absent,
Copper must be absent.
149
MANGANESE LOW RANGE
8.44 MANGANESE LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 300 µg/L (as Mn)
1 µg/L
±10 µg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the PAN Method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93748A-0
Manganese Low Range Reagent A
2 packets
HI93748B-0
Manganese Low Range Reagent B
0.40 mL
HI93748C-0
Manganese Low Range Reagent C
2 mL
HI93703-51
Dispersing Agent
6 drops
REAGENT SETS
Reagents for 50 tests
HI93748-01
HI93748-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Manganese LR method using the procedure
described in the Method Selection section (see page 19).
• Fill one cuvette (#1) with 10 mL of deionized water (up to the mark).
• Fill a second cuvette (#2) with 10 mL of sample (up to the mark).
• Add one packet of HI93748A-0 Manganese Low Range
Reagent A to each cuvette, replace the caps and shake
gently until completely dissolved.
150
10 mL
#1
10 mL
#2
• Add 1 mL of the HI93748C-0 Manganese Low Range
Reagent C to each cuvette, replace the caps and shake
gently.
MANGANESE LOW RANGE
• Add 0.2 mL of the HI93748B-0 Manganese Low Range
Reagent B to each cuvette, replace the caps and invert
gently to mix for about 30 seconds.
• Add 3 drops of HI93703-51 Dispersing Agent to
each cuvette, replace the caps and invert gently to
mix for about 30 seconds.
• Place the first cuvette (#1) with the reacted deionized
water into the holder and close the lid.
• Press Timer and the display will show the countdown prior to zeroing the blank. Alternatively
wait for 2 minutes and then press Zero. The display will show ”-0.0-” when the meter is zeroed
and ready for measurement.
151
MANGANESE LOW RANGE
• Insert the second cuvette (#2) with the reacted sample into the
instrument.
• Press Read to start the reading. The instrument displays the results in µg/L of manganese (Mn).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of potassium permanganate (KMnO4)
and permanganate (MnO4-).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Aluminum above 20 mg/L
Cadmium above 10 mg/L
Calcium above 200 mg/L as CaCO3
Cobalt above 20 mg/L
Copper above 50 mg/L
Iron above 10 mg/L
Lead above 0.5 mg/L
Magnesium above 100 mg/L as CaCO3
Nickel above 40 mg/L
Zinc above 15 mg/L
152
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 20.0 mg/L (as Mn)
0.1 mg/L
±0.2 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, Periodate method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93709A-0
Manganese High Range Reagent A
1 packet
HI93709B-0
Manganese High Range Reagent B
1 packet
MANGANESE HIGH RANGE
8.45. MANGANESE HIGH RANGE
REAGENT SETS
Reagents for 100 tests
HI93709-01
HI93709-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Manganese HR method using the procedure described in
the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.
• Remove the cuvette.
• Add one packet of HI93709A-0 Manganese High Range
Reagent A. Replace the cap and shake gently for
2 minutes to mix.
153
MANGANESE HIGH RANGE
• Add one packet of HI93709B-0 Manganese High Range
Reagent B. Replace the cap and shake gently for
2 minutes to mix.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 1 minute and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of manganese (Mn).
• Press  or  to access the second level functions.
• Press Chem Frm key to convert the result to mg/L potassium permanganate (KMnO4) and
permanganate (MnO4-).
• Press  or  to return to the measurement screen.
154
MANGANESE HIGH RANGE
INTERFERENCES
Interference may be caused by:
Calcium above 700 mg/L
Chloride above 70000 mg/L
Iron above 5 mg/L
Magnesium above 100000 mg/L
155
MOLYBDENUM
8.46. MOLYBDENUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 40.0 mg/L (as Mo6+)
0.1 mg/L
±0.3 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of the mercaptoacetic acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93730A-0
Molybdenum Reagent A
1 packet
HI93730B-0
Molybdenum Reagent B
1 packet
HI93730C-0
Molybdenum Reagent C
1 packet
REAGENT SETS
Reagents for 100 tests
HI93730-01
HI93730-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Molybdenum method using the procedure described in the
Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
156
25 mL
MOLYBDENUM
• Fill one graduated mixing cylinder up to the 25 mL mark
with the sample.
• Add one packet of HI93730A-0 Molybdenum Reagent A,
close the cylinder and invert several times until completely
dissolved.
• Add one packet of HI93730B-0 Molybdenum Reagent B to
the cylinder, close and invert several times until completely
dissolved.
• Add one packet of HI93730C-0 Molybdenum Reagent C to
the cylinder, close and shake vigorously.
• Fill an empty cuvette with 10 mL of reacted sample (up to
the mark) and replace the cap.
10 mL
• Insert the cuvette into the instrument and close the lid.
157
MOLYBDENUM
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in mg/L of molybdenum (Mo6+).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of molybdate (MoO42-) and sodium
molybdate (Na2MoO4).
• Press or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Aluminum above 50 mg/L
Chromium above 1000 mg/L
Copper above 10 mg/L
Iron above 50 mg/L
Nickel above 50 mg/L
Nitrite, as NO2¯
Sulfate above 200 mg/L
Highly buffered samples or with extreme pH may exceed the buffering capacity of the reagents
158
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.000 to 1.000 mg/L (as Ni)
0.001 mg/L
±0.010 mg/L ±7% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the PAN method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93740A-0
Nickel Low Range Reagent A
2 packets
HI93740B-0
Nickel Low Range Reagent B
2 mL
HI93740C-0
Nickel Low Range Reagent C
2 packets
HI93703-51
Dispersing Agent (optional reagent)
4-6 drops
NICKEL LOW RANGE
8.47. NICKEL LOW RANGE
REAGENT SETS
HI93740-01
Reagents for 50 tests
HI93740-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Nickel LR method using the procedure described in the
Method Selection section (see page 19).
Note: For best results samples should be between 20 and 24 °C.
• Fill one graduated beaker with 25 mL of deionized water (blank)
and another one with 25 mL of sample.
• Add one packet of HI93740A-0 Nickel Low Range Reagent A to each
beaker. Cap and swirl gently until the reagent is dissolved.
Note: If sample contains iron (Fe3+), it is important that all
powder is dissolved before continuing.
• Add 1 mL of HI93740B-0 Nickel Low Range Reagent B to each
beaker, and swirl to mix.
159
NICKEL LOW RANGE
• Press Timer and the display will show a countdown or alternatively, wait for 15 minutes.
•Add one packet of HI93740C-0 Nickel Low Range Reagent C
to each beaker, cap and swirl to mix until completely dissolved.
10 mL
•Fill one cuvette (#1) with 10 mL of the blank (up to the mark).
• Place the cuvette into the holder and close the lid.
#1
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
10 mL
• Fill a second cuvette (#2) with 10 mL of the reacted sample
(up to the mark).
10 mL
#2
• Insert the second cuvette into the instrument and close the lid.
160
Note: A temperature above 30 °C may cause turbidity. In this case add 2-3 drops of
HI93703-51 Dispersing Agent to each cuvette and swirl until turbidity is removed before
zeroing the meter and reading the sample.
NICKEL LOW RANGE
• Press Read to start the reading. The instrument displays the results in mg/L of nickel (Ni).
INTERFERENCES
Interference may be caused by:
Co2+ must not be present
Fe2+ must not be present
Al3+ above 32 mg/L
Ca2+ above 1000 mg/L (as CaCO3)
Cd2+ above 20 mg/L
Cl¯ above 8000 mg/L
Cr3+ above 20 mg/L
Cr6+ above 40 mg/L
Cu2+ above 15 mg/L
F¯ above 20 mg/L
Fe3+ above 10 mg/L
K+ above 500 mg/L
Mg2+ above 400 mg/L
Mn2+ above 25 mg/L
Mo6+ above 60 mg/L
Na+ above 5000 mg/L
Pb2+ above 20 mg/L
Zn2+ above 30 mg/L
161
NICKEL HIGH RANGE
8.48. NICKEL HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 7.00 g/L (as Ni)
0.01 g/L
±0.07g/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the photometric method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93726-0
Nickel High Range Reagent
1 packet
REAGENT SETS
HI93726-01
Reagents for 100 tests
HI93726-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Nickel HR method using the procedure described in the
Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
162
Remove the cuvette and add one packet of HI93726-0
Nickel High Range Reagent. Replace the cap and shake
gently until completely dissolved.
• Reinsert the cuvette into the instrument and close the lid.
NICKEL HIGH RANGE
•
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 1 minute and press Read. When the timer ends the meter will perform the reading. The
instrument displays the concentration in g/L of nickel (Ni).
INTERFERENCES
Interference may be caused by copper.
163
NITRATE
8.49. NITRATE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 30.0 mg/L (as NO3-- N)
0.1 mg/L
±0.5 mg/L ±10% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the cadmium reduction method
REQUIRED REAGENTS
Code
Description
HI93728-0
Nitrate Reagent
Quantity
1 packet
REAGENT SETS
HI93728-01
Reagents for 100 tests
HI93728-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Nitrate method using the procedure described in
the Method Selection section (see page 19).
• Fill the cuvette with 10 mL of sample, (up to the mark),
and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
164
NITRATE
• Remove the cuvette and add one packet of HI93728-0 Nitrate Reagent.
• Replace the cap and shake vigorously up and down for exactly
10 seconds. Continue to mix by inverting the cuvette gently for
50 seconds, while taking care not to induce air bubbles. Powder
will not completely dissolve. Time and method of shaking could
sensitively affect the measurement.
Note: The method is technique-sensitive. See procedure
on page 21 Cuvette Preparation for proper mixing
technique.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 4 minutes and 30 seconds and press Read. When the timer ends the meter will perform
the reading. The instrument displays the results in mg/L of nitrate-nitrogen (NO3-N).
• Press  or  to access the second level functions.
165
NITRATE
• Press the Chem Frm key to convert the result to mg/L of nitrate (NO3¯).
•Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Ammonia and amines, as urea and primary aliphatic amines
Chloride above 100 ppm
Chlorine above 2 ppm
Copper
Iron(III)
Strong oxidizing and reducing substances
Sulfide must be absent
166
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 30.0 mg/L Nitrate (as N03--N)
0.1 mg/L
±1.0 mg/L or ±3% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 420 nm
Chromotropic acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93766V-0*
Nitrate Reagent Vial
1 vial
HI93766-0
Nitrate Reagent1 packet
* Reagent Vial Identification: N, white label.
NITRATE (16 mm VIAL)
8.50. NITRATE (16 mm VIAL)
Note: Store the unused vials in a cool and dark place.
REAGENT SETS
HI93766-50
Reagents for 50 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions, and notes. Failure
to do so may result in serious injury to the operator.
• Select the Nitrate (16) method using the procedure described in the Method Selection section
(see page 19).
• Insert the 16 mm vial adapter using the procedure
described in the Using the 16 mm Vial Adapter section
(see page 22).
• Remove the cap from a HI93766V-0 Nitrate Reagent Vial.
• Add 1.0 mL of sample to the vial, while keeping the
vial at a 45-degree angle.
• Replace the cap and invert the vial 10 times. This is
blank.
WARNING: The vial will become hot during mixing. Use caution when handling.
167
NITRATE (16 mm VIAL)
Note: The method is technique sensitive. See procedure
on page 21 Cuvette Preparation for proper mixing
technique.
• Place the vial into the holder.
• Press Zero, the display will show “-0.0-” when the meter is zeroed and ready for measurement.
• Remove the vial.
• Add one packet of HI93766-0 Nitrate Reagent.
• Replace the cap and invert the vial 10 times. This is the reacted
sample.
Note: The method is technique sensitive. See procedure on
page 21 Cuvette Preparation for proper mixing technique.
• Place the vial into the holder.
• Press Timer and the display will show the countdown prior to the measurement or alternatively,
wait for 5 minutes and press Read. The instrument displays the concentration in mg/L of
nitrate-nitrogen (NO3--N).
168
NITRATE (16 mm VIAL)
• Press  or  to access the second level functions the Chem Frm key to convert the result in
mg/L of nitrate (NO3-).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Barium (Ba2+) above 1 mg/L
Chloride (Cl-) above 1000 mg/L
Nitrite (NO2-) above 50 mg/L
Samples containing up to 100 mg/L nitrite may be measured after the following treatment: add
400 mg of urea to 10 mL of sample, mix until completely dissolved, then proceed with the usual
measurement procedure.
169
NITRITE, MARINE ULTRA LOW RANGE
8.51. NITRITE, MARINE ULTRA LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 200 µg/L (as N02--N)
1 µg/L
±10 µg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the EPA Diazotization method 354.1
REQUIRED REAGENTS
CodeDescriptionQuantity
HI764-25
Nitrite Ultra Low Range Reagent
1 packet
REAGENT SETS
HI764-25
Reagents for 25 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Nitrite, Marine ULR method using the procedure
described in the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI764-25 Nitrite Ultra Low Range
Reagent. Replace the cap and shake gently for about
15 seconds.
170
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 15 minutes and press Read. When the timer ends the meter will perform the reading.
The instrument displays concentration in µg/L of nitrite-nitrogen (NO2--N).
NITRITE, MARINE ULTRA LOW RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of nitrite (NO2¯) and sodium nitrite
(NaNO2).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by the following ions: ferrous, ferric, cupric, mercurous, silver,
antimonious, bismuth, auric, lead, metavanadate and chloroplatinate.
Strongly reducing and oxidizing reagents.
High levels of nitrate (above 100 mg/L) could yield falsely high readings due to a minute amount
of reduction to nitrite that could occur at these levels.
171
NITRITE LOW RANGE
8.52. NITRITE LOW RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 600 µg/L (as NO2--N)
1 µg/L
±20 µg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the EPA Diazotization method 354.1
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93707-0
Nitrite Low Range Reagent
1 packet
REAGENT SETS
HI93707-01
Reagents for 100 tests
HI93707-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Nitrite LR method using the procedure described
in the Method Selection section (see page 19).
• Fill the cuvette up to the mark with 10 mL of unreacted
sample (up to the mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
172
• Reinsert the cuvette into the instrument and close the lid.
NITRITE LOW RANGE
• Add one packet of HI93707-0 Nitrite Low Range Reagent.
Replace the cap and shake gently for about 15 seconds.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 15 minutes and press Read. When the timer ends the meter will perform the reading.
The instrument displays concentration in µg/L of nitrite-nitrogen (NO2--N).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of nitrite (NO2-) and sodium nitrite
(NaNO2).
• Press  or  to return to the measurement screen.
173
NITRITE LOW RANGE
174
INTERFERENCES
Interference may be caused by the following ions: ferrous, ferric, cupric, mercurous, silver,
antimonious, bismuth, auric, lead, metavanadate and chloroplatinate. Strongly reducing and
oxidizing reagents.
High levels of nitrate (above 100 mg/L) could yield falsely high readings due to a minute amount
of reduction to nitrite that could occur at these levels.
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 150 mg/L (as NO2-)
1 mg/L
±4 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the Ferrous Sulfate method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93708-0
Nitrite High Range Reagent
1 packet
NITRITE HIGH RANGE
8.53. NITRITE HIGH RANGE
REAGENT SETS
HI93708-01
Reagents for 100 tests
HI93708-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Nitrite HR method using the procedure described
in the Method Selection section (see page 19).
10 mL
• Fill the cuvette up to the mark with 10 mL of unreacted
sample (up to the mark) and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
175
NITRITE HIGH RANGE
• Add one packet of HI93708-0 Nitrite High Range Reagent.
Replace the cap and shake gently until completely
dissolved.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 10 minutes and press Read. When the timer ends the meter will perform the reading.
The instrument displays concentration in mg/L of nitrite (NO2-).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of nitrite-nitrogen (NO2--N) and
sodium nitrite (NaNO2).
• Press  or  to return to the measurement screen.
176
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 25.0 mg/L (as N)
0.1 mg/L
±1.0 mg/L or ±5% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 420 nm
Chromotropic acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93767A-B*
Total Nitrogen Low Range Digestion Vial
2 vials
Deionized Water
2 mL
DEIONIZED120
PERSULFATE/N
Potassium Persulfate Reagent
2 packets
BISULFITE/N
Sodium Metabisulfite Reagent
2 packets
HI93767-0
Total Nitrogen Reagent
2 packets
Total Nitrogen Low Range Reagent Vial
2 vials
HI93766V-0LR**
* Reagent Vial identification: N LR, green label
** Reagent Vial identification: N LR, red label
NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
8.54. NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
Notes: Store the unused vials in their container in a cool and dark place.
REAGENT SETS
HI93767A-50 Reagents for up to 49 tests.
Box 1: HI93767A-50 Reagent Set
Box 2: HI93767A&B-50 Reagent Set, for Nitrogen Total Low Range.
For other accessories see page 267.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions and notes. Failure
to do so may result in serious injury to the operator.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may
be used more than once, the blank vial is stable for one week if stored in a dark place at room
temperature. For improved accuracy use the same lot of reagents for the blank and sample, and
run a blank for each set of measurements.
• Preheat the HANNA® Reactor HI839800 to 105 °C (221 °F). The optional HI740217 safety
shield is strongly recommended.
177
NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and
possibly explosive atmosphere.
• Remove the cap from two HI93767A-B Total Nitrogen Low Range Digestion Vials.
• Add one packet of Persulfate/N, Potassium Persulfate to each vial.
• Add 2 mL of deionized water to the first vial (#1, blank) and 2 mL of sample to the second vial
(#2, sample), while keeping the vials at a 45-degree angle.
• Replace the cap and shake vigorously for 30 seconds or until powder is completely dissolved.
178
WARNING: The vials are still hot, use caution when handling.
• Select Nitrogen Total LR (16) method using the procedure described in Method Selection section
(see page 19).
• Insert 16 mm vial adapter using the procedure described in the Using the 16 mm Vial Adapter
section (see Page 22).
• For this method the instrument provides 3 reaction timers which can be used troughout the
procedure.
• Remove the cap from the vials and add one packet of BISULFITE/N Sodium Metabisulfite
analysis to each vial. Replace the cap and shake gently for 15 seconds.
NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
• Insert the vials into the reactor and heat them for 30 minutes at 105 °C.
Note: To obtain most accurate results, it is strongly
recommended to remove the vials from the reactor after
30 minutes.
• At the end of the digestion period switch off the reactor, place the
vials in the test tube rack and allow to cool to room temperature.
• Press Timer and the display will show the countdown prior to adding HI93767-0 Total Nitrogen
Reagent, or alternatively wait 3 minutes.
179
NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
• Remove the cap from the vials and add one packet of HI93767-0 Total Nitrogen Reagent to
each vial. Replace the cap and shake gently for 15 seconds.
• Press Continue and the display will show the countdown, or alternatively wait 2 minutes
(without shaking the vials) to allow the reaction to complete.
• Remove the cap from two HI93766V-0LR Total Nitrogen Low Range Reagent Vial.
• Add 2 mL of digested blank (#1) to one of the reagent vials and 2 mL of digested sample
(#2) to the second reagent vial, while keeping the vials at a 45-degree angle.
• Replace the cap and invert 10 times.
WARNING: The vials will become hot during mixing, use caution when handling.
Note: The method is technique sensitive. See procedure on page 21 Cuvette preparation for
proper mixing technique.
180
• Place the blank vial (#1) into the holder
• Press Continue and the display will show the countdown, or alternatively wait for 5 minutes.
• Press the Zero key. The display will show "-0.0-" when the meter is zeroed and ready for
measurement.
NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
#1
#2
• Remove the blank vial.
• Place the sample vial (#2) into the holder.
• Press Read key to start the reading. The instrument displays the results in mg/L of nitrogen (N).
• Press  or  to access the second level functions.
181
NITROGEN, TOTAL LOW RANGE (16 mm VIAL)
182
• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and nitrate (NO3-).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Bromide (Br -) above 60 mg/L
Chloride (Cl-) above 1000 mg/L
Chromium (Cr3+) above 0.5 mg/L
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 150 mg/L (as N)
1 mg/L
±3 mg/L or ±4% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 420 nm
Chromotropic acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93767B-B*
Total Nitrogen High Range Digestion Vial
2 vials
DEIONIZED120
Deionized Water
0.5 mL
PERSULFATE/N
Potassium Persulfate Reagent 2 packets
BISULFITE/N
Sodium Metabisulfite Reagent
2 packets
HI93767-0
Total Nitrogen Reagent
2 packets
2 vials
HI93766V-0HR** Total Nitrogen High Range Reagent Vial
* Reagent Vial identification: N HR, red label
** Reagent Vial identification: N HR, green label
Notes: Store the unused vials in their container in a cool and dark place.
NITROGEN, TOTAL HIGH RANGE (16 mm VIAL)
8.55. NITROGEN, TOTAL HIGH RANGE (16 mm VIAL)
REAGENT SETS
HI93767B-50 Reagents for up to 49 tests.
Box 1: HI93767B-50 Reagent Set
Box 2: HI93767A&B-50 Reagent Set, for Nitrogen Total High Range.
For other accessories see page 267.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions and notes. Failure
to do so may result in serious injury to the operator.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may
be used more than once, the blank vial is stable for one week if stored in a dark place at room
temperature. For improved accuracy always use the same lot of reagents for the blank and sample,
and run a blank for each set of measurements.
• Preheat the HANNA® Reactor HI839800 to 105 °C (221 °F). The optional HI740217 safety
shield is strongly recommended.
183
NITROGEN, TOTAL HIGH RANGE (16 mm VIAL)
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and
possibly explosive atmosphere.
• Remove the cap from two HI93767B-B Total Nitrogen High Range Digestion Vials.
• Add one packet of PERSULFATE/N, Potassium Persulfate to each vial.
• Add 0.5 mL of deionized water to the first vial (#1, blank) and 0.5 mL of sample to the second
vial (#2, sample), while keeping the vials at a 45-degree angle.
• Replace the cap and shake vigorously for about 30 seconds or until powder is completely
dissolved.
• Insert the vials into the reactor and heat them for 30 minutes at
105°C.
Note: To obtain most accurate results, it is strongly recommended to remove the vials from
the reactor after 30 minutes.
184
WARNING: The vials are still hot, use caution when handling.
• Select Nitrogen Total HR (16) method using the procedure described in Method selection section
(page 19).
• Insert the 16 mm vial adapter using the procedure described in the Using the 16 mm Vial
Adapter section (see page 22).
• For this method the instrument provides 3 reaction timers which can be used throughout the
procedure.
• Remove the cap from the vials and add one packet of BISULFITE/N, Sodium Metabisulfite to
each vial. Replace the cap and shake gently for 15 seconds.
NITROGEN, TOTAL HIGH RANGE (16 mm VIAL)
• At the end of the digestion place the vials in the test tube rack and
allow to cool to room temperature.
• Press Timer and the display will show the countdown prior to adding HI93767-0 Total Nitrogen
Reagent, or alternatively wait 3 minutes.
• Remove the cap from the vials and add one packet of HI93767-0 Total Nitrogen
Reagent to each vial. Replace the cap and shake gently for 15 seconds.
185
NITROGEN, TOTAL HIGH RANGE (16 mm VIAL)
• Press Continue and the display will show the countdown, or alternatively wait 2 minutes.
• Remove the cap from two HI93766V-0HR Total Nitrogen High Range Regent Vials.
• Add 2 mL of digested blank (#1) to one of the reagent vials and 2 mL of digested sample
(#2) to the second reagent vial, while keeping the vials at a 45-degree angle.
• Replace the cap tightly and invert the vials 10 times.
WARNING: The vials will become hot during mixing, use caution when handling.
Note: The method is technique sensitive, see procedure
on page 21 Cuvette preparation for proper mixing
technique.
#1
• Place the sample vial (#1) into the holder.
• Press Continue and the display will show the countdown, or alternatively wait for 5 minutes.
186
#2
• Remove the blank vial.
• Place the sample vial (#2) into the holder.
• Press Read to start the reading. The instrument displays the results in mg/L nitrogen (N).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of ammonia (NH3) and nitrate
(NO3¯).
NITROGEN, TOTAL HIGH RANGE (16 mm VIAL)
• Press the Zero key. The the display will show “-0.0-”.
• Press  or  to return to the measurement screen.
The method detects all organic and inorganic forms of nitrogen present in the sample.
INTERFERENCES
Interference may be caused by:
Bromide (Br¯) above 240 mg/L
Chloride (Cl¯) above 3000 mg/L
Chromium (Cr3+) above 0.5 mg/L
187
OXYGEN, DISSOLVED
8.56. OXYGEN, DISSOLVED
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 10.0 mg/L (as O2)
0.1 mg/L
±0.4 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 420 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, Azide modified Winkler method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93732A-0
Dissolved Oxygen Reagent A
5 drops
HI93732B-0
Dissolved Oxygen Reagent B
5 drops
HI93732C-0
Dissolved Oxygen Reagent C
10 drops
REAGENT SET
Reagents for 100 tests
HI93732-01
HI93732-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Oxygen (dissolved) method using the procedure
described in the Method Selection section (see page 19).
• Fill one 60 mL glass bottle completely with the unreacted
sample.
• Replace the cap and ensure that a small part of the sample
spills over.
• Remove the cap and add 5 drops of HI93732A-0 and 5 drops of
HI93732B-0.
• Add more sample, to fill the bottle completely. Replace the cap
and ensure that a part of the sample spills over.
Note: This ensures no air bubbles have been trapped inside
the bottle. Trapped air bubbles could alter readings.
• Invert the bottle several times until the sample turns orangeyellow and a flocculating agent appears.
188
OXYGEN, DISSOLVED
• Let the sample stand for approximately 2 minutes to allow flocculating
agent to settle.
• When the upper half of the bottle is clear, add 10 drops of
HI93732C-0 Dissolved Oxygen Reagent C.
• Replace the cap and invert the bottle until the settled flocculating
agent dissolves completely. The sample is ready for measurement
when it is yellow and completely clear.
• Fill the first cuvette (#1) with 10 mL of the unreacted sample
(up to the mark), and replace the cap.
• Place the cuvette into the holder and close the lid.
10 mL
#1
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Fill second cuvette (#2) with 10 mL of the reacted sample
(up to the mark) and replace the cap.
189
OXYGEN, DISSOLVED
#2
• Insert the cuvette into the holder and close the lid.
• Press Read to start the reading. The instrument will display the results in mg/L of oxygen (O2).
INTERFERENCES
Interferences may be caused by reducing and oxidizing materials.
190
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
OXYGEN SCAVENGERS (CARBOHYDRAZIDE)
8.57. OXYGEN SCAVENGERS (CARBOHYDRAZIDE)
0.00 to 1.50 mg/L (as Carbohydrazide)
0.01 mg/L
±0.02 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the iron reduction method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96773A-0
Oxygen Scavengers Reagent A 2 packets
HI96773B-0
Oxygen Scavengers Reagent B 1 mL
REAGENT SET
HI96773-01
Reagents for 50 tests
HI96773-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Oxy. Scavengers (Carbohy) method using the procedure
described in the Method Selection section (see page 19).
10 mL
• Fill first cuvette (#1) with 10 mL of deionized water (up to the mark).
• Fill second cuvette (#2) with 10 mL of sample (up to the mark).
• Add one packet of HI96773A-0 Oxygen Scavengers
Reagent A to cuvette #1. Replace the cap and
invert for 30 seconds.
#1
10 mL
#2
#1
191
OXYGEN SCAVENGERS (CARBOHYDRAZIDE)
• Add one packet of HI96773A-0 Oxygen Scavengers
Reagent A to cuvette #2. Replace the cap and
invert for 30 seconds.
#2
• Add 0.5 mL of HI96773B-0 Oxygen Scavengers
Reagent B to each cuvette using the 1 mL syringe.
#2
#1
• Replace the caps and invert for 10 seconds.
#1
• Place first cuvette (#1) into the holder and close the lid.
• Press Timer and the display will show countdown prior to the measurement or, alternatively,
wait for 10 minutes and press Zero. The display will show “-0.0-“ when the meter is zeroed
and ready for measurement.
192
• Insert the second cuvette (#2) into the instrument and close the lid.
#2
• Press Read to start reading. The instrument displays the results in mg/L of carbohydrazide.
INTERFERENCES
Interference may be caused by:
Borate (as Na2B4O7), Cobalt, Copper, Iron (Ferrous), Hardness (as CaCO3), Light, Lignosulfonates,
Manganese, Molybdenum, Nickel, Phosphate, Phosphonates, Sulfate, Temperature, and Zinc.
OXYGEN SCAVENGERS (CARBOHYDRAZIDE)
• Remove the cuvette.
193
OXYGEN SCAVENGERS (DEHA)
8.58. OXYGEN SCAVENGERS (DEHA)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 1000 µg/L (as DEHA)
1 µg/L
±5 µg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the iron reduction method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96773A-0
Oxygen Scavengers Reagent A 2 packets
HI96773B-0
Oxygen Scavengers Reagent B 1 mL
REAGENT SET
HI96773-01
Reagents for 50 tests
HI96773-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Oxy. Scavengers (DEHA) method using the procedure
described in the Method Selection section (see page 19).
• Fill first cuvette (#1) with 10 mL of deionized water (up to the mark).
194
#1
10 mL
• Fill second cuvette (#2) with 10 mL of sample (up to the mark).
• Add one packet of HI96773A-0 Oxygen Scavengers
Reagent A to #1 cuvette. Replace the cap and
invert for 30 seconds.
10 mL
#2
#1
OXYGEN SCAVENGERS (DEHA)
• Add one packet of HI96773A-0 Oxygen Scavengers
Reagent A to #2 cuvette. Replace the cap and
invert for 30 seconds.
#2
• Add 0.5 mL of HI96773B-0 Oxygen Scavengers
Reagent B to each cuvette using the 1 mL syringe.
#2
#1
• Replace the caps and invert for 10 seconds.
• Place first cuvette (#1) into the holder and close the lid.
#1
• Press Timer and the display will show countdown prior to the measurement or, alternatively,
wait for 10 minutes and press Zero. The display will show “-0.0-“ when the meter is zeroed
and ready for measurement.
195
OXYGEN SCAVENGERS (DEHA)
• Remove the cuvette.
#2
• Insert the second cuvette (#2) into the holder and close the lid.
• Press Read to start reading. The instrument displays the results in µg/L of DEHA.
INTERFERENCES
Interference may be caused by:
Borate (as Na2B4O7), Cobalt, Copper, Iron (Ferrous), Hardness (as CaCO3), Light, Lignosulfonates,
Manganese, Molybdenum, Nickel, Phosphate, Phosphonates, Sulfate, Temperature, and Zinc.
196
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
OXYGEN SCAVENGERS (HYDROQUINONE)
8.59. OXYGEN SCAVENGERS (HYDROQUINONE)
0.00 to 2.50 mg/L (as Hydroquinone)
0.01 mg/L
±0.04 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the iron reduction method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96773A-0
Oxygen Scavengers Reagent A 2 packets
HI96773B-0
Oxygen Scavengers Reagent B 1 mL
REAGENT SET
HI96773-01
Reagents for 50 tests
HI96773-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Oxy. Scavengers (Hydro) method using the procedure
described in the Method Selection section (see page 19).
• Fill first cuvette (#1) with 10 mL of deionized water (up to the mark).
#1
10 mL
• Fill second cuvette (#2) with 10 mL of sample (up to the mark).
• Add one packet of HI96773A-0 Oxygen Scavengers
Reagent A to #1 cuvette. Replace the cap and
invert for 30 seconds.
10 mL
#2
#1
197
OXYGEN SCAVENGERS (HYDROQUINONE)
• Add one packet of HI96773A-0 Oxygen Scavengers
Reagent A to #2 cuvette. Replace the cap and
invert for 30 seconds.
#2
• Add 0.5 mL of HI96773B-0 Oxygen Scavengers
Reagent B to each cuvette using the 1 mL syringe.
#2
#1
• Replace the caps and invert for 10 seconds.
#1
• Place first cuvette (#1) into the holder and close the lid.
• Press Timer and the display will show countdown prior to the measurement or, alternatively,
wait for 2 minutes and press Zero. The display will show “-0.0-“ when the meter is zeroed and
ready for measurement.
198
• Insert the second cuvette (# 2) into the holder and close the lid.
#2
• Press Read to start reading. The instrument displays the results in mg/L of hydroquinone.
OXYGEN SCAVENGERS (HYDROQUINONE)
• Remove the cuvette.
INTERFERENCES
Interference may be caused by:
Borate (as Na2B4O7), Cobalt, Copper, Iron (Ferrous), Hardness (as CaCO3), Light, Lignosulfonates,
Manganese, Molybdenum, Nickel, Phosphate, Phosphonates, Sulfate, Temperature, and Zinc.
199
OXYGEN SCAVENGERS (ISO-ASCORBIC ACID)
8.60. OXYGEN SCAVENGERS (ISO-ASCORBIC ACID)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 4.50 mg/L (as Iso-ascorbic acid)
0.01 mg/L
±0.03 mg/L ±3 % of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of the iron reduction method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96773A-0
Oxygen Scavengers Reagent A 2 packets
HI96773B-0
Oxygen Scavengers Reagent B 1 mL
REAGENT SET
HI96773-01
Reagents for 50 tests
HI96773-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Oxy. Scavengers (ISA) method using the procedure
described in the Method Selection section (see page 19).
10 mL
• Fill first cuvette (#1) with 10 mL of deionized water (up to the mark).
#1
10 mL
• Fill second cuvette (#2) with 10 mL of sample (up to the mark).
• Add one packet of HI96773A-0 Oxygen Scavengers Reagent A
to #1 cuvette. Replace the cap and invert for 30 seconds.
200
#2
#1
#2
• Add 0.5 mL of HI96773B-0 Oxygen Scavengers
Reagent B to each cuvette using the 1 mL syringe.
#2
#1
• Replace the caps and invert for 10 seconds.
• Place first cuvette (#1) into the holder and close the lid.
OXYGEN SCAVENGERS (ISO-ASCORBIC ACID)
• Add one packet of HI96773A-0 Oxygen Scavengers Reagent A
to #2 cuvette. Replace the cap and invert for 30 seconds.
#1
• Press Timer and the display will show countdown prior to the measurement or, alternatively,
wait for 10 minutes and press Zero. The display will show “-0.0-“ when the meter is zeroed
and ready for measurement.
201
OXYGEN SCAVENGERS (ISO-ASCORBIC ACID)
202
• Remove the cuvette.
#2
• Insert the second cuvette (#2) into the instrument and close the lid.
• Press Read to start reading. The instrument displays the results in mg/L of Iso-ascorbic acid.
INTERFERENCES
Interference may be caused by:
Borate (as Na2B4O7), Cobalt, Copper, Iron (Ferrous), Hardness (as CaCO3), Light, Lignosulfonates,
Manganese, Molybdenum, Nickel, Phosphate, Phosphonates, Sulfate, Temperature, and Zinc.
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
OZONE
8.61. OZONE
0.00 to 2.00 mg/L (as O3)
0.01 mg/L
±0.02 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Colorimetric DPD Method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93757-0
Ozone Reagent1 packet
HI93703-52-0
Glycine Powder (Optional Reagent)
1 packet
REAGENT SETS
HI93757-01
Reagents for 100 tests
HI93757-03
Reagents for 300 tests
HI93703-52
Reagents for 100 tests (Optional)
For other accessories see page 267.
Note: If the sample is suspected to contain chlorine residue (free or total chlorine), follow
the alternative measurement procedure described below, chlorine is a strong interferent.
• Perform the Standard Measurement Procedure. Record the result as Value A.
• Perform Additional Measurement Procedure. Record the result, Value B.
• To determine the ozone concentration in mg/L subtract Value B from Value A.
mg/L ozone (O3) = Value A – Value B.
STANDARD MEASUREMENT PROCEDURE
• Select the Ozone method using the procedure described in the
Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to the
mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
203
OZONE
• Press the Zero key. The display will show “-0.0-” the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93757-0 Ozone Reagent. Replace
the cap and shake gently for 20 seconds.
• Replace the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays the result in mg/L ozone (O3) (chlorine free sample only).
10 mL
Note: For samples containing chlorine, record this value as A.
ADDITIONAL MEASUREMENT PROCEDURE
For samples containing chlorine
• Fill the cuvette with 10 mL of unreacted sample (up to the mark).
• Place the cuvette into the holder and close the lid.
204
OZONE
• Press the Zero key. The display will show “-0.0-” the meter is zeroed and ready for measure.
• Remove the cuvette.
• Add one packet of the HI93703-52-0 Glycine Powder.
Replace the cap and shake gently until completely
dissolved.
#1
• Add one packet of HI93757-0 Ozone Reagent. Replace
the cap and shake gently for 20 seconds.
#2
• Replace the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
Record this value as B.
205
OZONE
206
• To determine the mg/L ozone (O3) concentration in sample containing chlorine subtract value B
(additional measurement procedure) from value A (standard measurement procedure).
INTERFERENCES
Interference may be caused by: Bromine, Chlorine Dioxide, Iodine.
Alkalinity above 250 mg/L CaCO3 will not reliably develop the full amount of color or it may rapidly
fade. To resolve this, neutralize the sample with diluted HCl.
In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately
2 minutes after adding the powder reagent.
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
pH
8.62. pH
6.5 to 8.5 pH
0.1 pH
±0.1 pH at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of the Phenol Red method
REQUIRED REAGENTS
Code
Description
HI93710-0
pH Reagent
Quantity
5 drops
REAGENT SETS
HI93710-01
Reagents for 100 pH tests
HI93710-03
Reagents for 300 pH tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the pH method using the procedure described in
the Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample (up to
the mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
207
pH
• Remove the cuvette and add 5 drops of HI93710-0
pH Reagent Indicator. Replace the cap and mix the
solution.
• Reinsert the cuvette into the instrument and close the lid.
• Press the Read key to start the reading. The instrument displays the result in pH.
208
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 200 µg/L (as P)
1 µg/L
±5 µg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 20th edition, Ascorbic Acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI736-25
Phosphorus Ultra Low Range Reagent
1 packet
REAGENT SETS
HI736-25
Reagents for 25 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Phosphate Marine ULR method using the procedure described in the Method Selection
section (see page 19).
PHOSPHATE, MARINE ULTRA LOW RANGE
8.63. PHOSPHATE, MARINE ULTRA LOW RANGE
• Rinse, cap and shake the cuvette several times with
unreacted sample.
• Fill the cuvette with 10 mL of sample (up to the
mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Add one packet of HI736-25 Phosphorus Ultra Low
Range Reagent. Replace the cap and shake gently
(for about 2 minutes) until the powder is completely
dissolved.
209
PHOSPHATE, MARINE ULTRA LOW RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in µg/L of phosphorus (P).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to µg/L of phosphate (PO43-) and phosphorus
pentoxide (P2O5).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Iron above 50 mg/L
Silica above 50 mg/L
Silicate above 10 mg/L
Copper above 10 mg/L
Hydrogen sulfide, arsenate, turbid sample and highly buffered samples also interfere.
210
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.50 mg/L (as PO43-)
0.01 mg/L
±0.04 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Adaptation of the Ascorbic Acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93713-0
Phosphate Low Range Reagent 1 packet
PHOSPHATE LOW RANGE
8.64. PHOSPHATE LOW RANGE
REAGENT SETS
HI93713-01
Reagents for 100 tests
HI93713-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
10 mL
• Select the Phosphate LR method using the procedure
described in the Method Selection section (see page 19).
• Rinse, cap and shake the cuvette several times with
unreacted sample.
• Fill the cuvette with 10 mL of sample (up to the mark) and
replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette and add the content of one packet
of HI93713-0 Phosphate Low Range Reagent. Replace
the cap and shake gently (for about 2 minutes) until
the powder is completely dissolved.
211
PHOSPHATE LOW RANGE
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays concentration in mg/L of phosphate (PO43¯).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of phosphorus (P) and phosphorus
pentoxide (P2O5).
• Press or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Iron above 50 mg/L
Silica above 50 mg/L
Silicate above 10 mg/L
Copper above 10 mg/L
Hydrogen sulfide, arsenate, turbid sample and highly buffered samples also interfere.
212
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.0 to 30.0 mg/L (as PO43-)
0.1 mg/L
±1.0 mg/L ±4% of reading at 25 °C
LED with narrow band interference filter @ 525 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93717A-0
Phosphate High Range Reagent A 10 drops
HI93717B-0
Phosphate High Range Reagent B
1 packet
PHOSPHATE HIGH RANGE
8.65. PHOSPHATE HIGH RANGE
REAGENT SETS
Reagents for 100 tests
HI93717-01
HI93717-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Phosphate HR method using the
procedure described in the Method Selection
section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample
(up to the mark) and replace the cap.
10 mL
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement.
• Add 10 drops of HI93717A-0 Phosphate High Range Reagent A.
213
PHOSPHATE HIGH RANGE
• Add one packet of HI93717B-0 Phosphate HR Reagent
B to the cuvette. Replace the cap and shake gently
until completely dissolved.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The
instrument displays the results in mg/L of phosphate (PO43¯).
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of phosphorus (P) and phosphorus
pentoxide (P2O5).
• Press  or  to return to the measurement screen.
214
PHOSPHATE HIGH RANGE
INTERFERENCES
Interference may be caused by:
Sulfide
Chloride above 150000 mg/L
Calcium above 10000 mg/L as CaCO3
Magnesium above 40000 mg/L as CaCO3
Ferrous iron above 100 mg/L
215
PHOSPHORUS, REACTIVE LOW RANGE (16 mm VIAL)
8.66. PHOSPHORUS, REACTIVE LOW RANGE (16 mm VIAL)
SPECIFICATIONS
Range
0.00 to 1.60 mg/L (as P)
Resolution
0.01 mg/L
Accuracy
±0.05 mg/L or ±4% of reading at 25 °C, whichever is greater
Light Source
LED with narrow band interference filter @ 610 nm
Method
Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic
acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93758A-0*
Phosphorus Reactive Reagent Vial
1 vial
HI93758-0
Phosphorus Reagent1 packet
* Reagent Vial identification: P R, red label
REAGENT SETS
HI93758A-50
Reagents for 50 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Phosphorus React. LR (16) method using the procedure described
in the Method Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in
the Using 16 mm Vial Adapter section (see page 22).
• Remove the cap from HI93758A-0 Reactive Phosphorus
Reagent Vial.
• Add 5.0 mL of sample to the vial, while keeping the
vial at a 45-degree angle.
• Replace the cap and invert several times to mix.
• Place the vial into the holder.
216
• Remove the vial.
• Remove the cap and add one packet of HI93758-0
Phosphorus Reagent.
• Replace the cap shake gently for 2 minutes until most of
the powder is dissolved.
• Place the vial into the holder.
• Press Timer and the display will show the countdown prior to the measurement, or alternatively
wait for 3 minutes and press Read. When the timer ends the meter will perform the reading.The
instrument displays the results in mg/L of Phosphorous (P).
PHOSPHORUS, REACTIVE LOW RANGE (16 mm VIAL)
• Press the Zero key. The display will show "-0.0-" when the meter is zeroed and ready for
measurement.
• Press  or  to access the second level functions.
217
PHOSPHORUS, REACTIVE LOW RANGE (16 mm VIAL)
218
• And press the Chem Frm key to convert the result to mg/L of phosphate (PO43¯) and
phosphorus pentoxide (P2O5).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Arsenate at any level
Silica above 50 mg/L
Sulfide above 6 mg/L
To eliminate sulfide: add Bromine Water drop-wise until a pale yellow color develops; remove
Bromine Water excess by adding Phenol solution drop-wise.
Turbidity and suspended matter in large amounts may cause interference because the reaction
conditions may dissolve suspended matter or cause desorption of phosphates from particles.
Turbidity or suspended matter should be removed before measurement by treatment with active
carbon and by prior filtration.
SPECIFICATIONS
Range
0.0 to 32.6 mg/L (as P)
Resolution
0.1 mg/L
Accuracy
±0.5 mg/L or ±4% of reading at 25 °C, whichever is greater
Light Source
LED with narrow band interference filter @ 420 nm
Method
Adaptation of Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P C, vanadomolybdophosphoric acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93763A-0*
Reactive Phosphorus High Range Reagent Vial
2 vials
DEIONIZED120
Deionized Water5 mL
*Reagent vial Identification: P RHR, green label
Note: Store the unused vials in their container in a cool and dark place.
REAGENT SETS
Reagents for up to 49 tests
HI93763A-50
For other accessories see page 267.
MEASUREMENT PROCEDURE
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial
may be used more than once; the blank vial is stable up to two weeks (room temperature). For
improved accuracy always use the same lot of reagents for the blank and sample, and run a blank
for each set of measurements.
• Select the Phosphorus React. HR (16) method using the procedure described in the Method
Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in the Using the 16 mm Vial
Adapter section (see page 22).
• Remove the cap from two HI93763A-0 Phosphorus Reactive High Range Reagent Vials.
PHOSPHORUS, REACTIVE HIGH RANGE (16 mm VIAL)
8.67. PHOSPHORUS, REACTIVE HIGH RANGE (16 mm VIAL)
• Add 5 mL of deionized water to the first vial (#1) and 5 mL of sample to the second vial (#2),
while keeping the vials at a 45-degree angle.
219
PHOSPHORUS, REACTIVE HIGH RANGE (16 mm VIAL)
• Replace the cap and invert several times to mix.
#1
• Place the blank vial (#1) into the holder and push it
completely down.
• Press Timer and the display will show the countdown prior to the zero reading, or alternatively
wait 7 minutes and press Zero. The display will show "-0.0-" when the meter is zeroed and
ready for measurement.
#2
• Remove the blank vial.
• Place the sample vial (#2) into the holder.
• Press Read to start the measurement. The instrument displays the results in mg/L of phosphorus (P).
220
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Bismuth
Fluoride
pH: the sample should have a neutral pH
Sulfide: to eliminate sulfide add Bromine Water drop-wise until a pale yellow color develops;
remove Bromine
Water excess by adding Phenol solution drop-wise.
Temperature: the method is temperature sensitive.
It is recommended to run measurements at T = 20 to 25 °C:
T < 20 °C causes a negative error
T > 25 °C causes a positive error
Turbidity and suspended matter in large amounts may cause interference because the strongly
acidic reaction conditions may dissolve suspended matter or cause desorption of phosphates from
particles. Before measurement, turbidity or suspended matter should be removed by treatment with
active carbon and by prior filtration.
PHOSPHORUS, REACTIVE HIGH RANGE (16 mm VIAL)
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of phosphate (PO4 3¯) and
phosphorus pentoxide (P2O5).
221
PHOSPHORUS, ACID HYDROLYZABLE (16 mm VIAL)
8.68. PHOSPHORUS, ACID HYDROLYZABLE (16 mm VIAL)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 1.60 mg/L (as P)
0.01 mg/L
±0.05 mg/L or ±5% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 610 nm
Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93758V-0AH*
Phosphorus Reagent Vial
1 vial
HI93758B-0
NaOH Solution 1.20N
2 mL
HI93758-0
Phosphorous Reagent1 packet
* Reagent vial Identification: P AH, white label
Note: Store the unused vials in their container in a cool and dark place.
REAGENT SETS
Reagents for 50 tests
HI93758B-50
For other accessories see page 267.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions and notes. Failure to
do so may result in serious injury to the operator.
• Preheat the HANNA® Reactor HI839800 to 150 °C (302°F). The optional HI740217 safety
shield is strongly recommended.
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and
possibly explosive atmosphere.
222
• Add 5.0 mL of sample to the vial, while keeping the vial at a
45-degree angle.
• Replace the cap and invert to mix.
• Insert the vial into the reactor and heat it for 30 minutes at 150°C.
• At the end of the digestion place the vials carefully in the test tube
rack and allow to cool to room temperature.
PHOSPHORUS, ACID HYDROLYZABLE (16 mm VIAL)
• Remove the cap from a HI93758V-0AH Phosphorus Reagent Vial.
WARNING: The vials are still hot, use caution when handling.
• Select the Phosphorus Acid Hydr. (16) method using the procedure described in the Method
Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in the
Using the 16 mm vial Adapter section (see page 22).
• Remove the cap from the vial and add 2.0 mL of HI93758B-0
NaOH Solution 1.20N while keeping the vial at a
45-degree angle.
• Replace the cap and invert to mix.
223
PHOSPHORUS, ACID HYDROLYZABLE (16 mm VIAL)
• Place the vial into the holder.
• Press Zero key. The display will show “-0.0-”. Now the meter is zeroed and ready for
measurement.
• Remove the vial.
• Remove the cap and add one packet of HI93758-0 Phosphorus
Reagent.
• Replace the cap and shake gently for 2 minutes until most of the
powder is dissolved.
• Place the vial into the holder.
• Press Timer and the display will show the countdown prior to the measurement, or alternatively wait
for 3 minutes and press Read. The instrument displays the results in mg/L of phosphorus (P).
224
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result in mg/L of phosphate (PO43¯) and mg/L
phosphorus pentoxide (P2O5).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Arsenate at any level
Silica above 50 mg/L
Sulfide above 9 mg/L
To eliminate sulfide: add Bromine Water drop-wise until a pale yellow color develops; remove
Bromine Water excess by adding Phenol solution drop-wise.
Turbidity and suspended matter in large amounts may cause interference because the strongly
acidic reaction conditions may dissolve suspended matter or cause desorption of phosphates from
particles. Before measurement, turbidity or suspended matter should be removed by treatment with
active carbon and by prior filtration.
PHOSPHORUS, ACID HYDROLYZABLE (16 mm VIAL)
Note: The method detects free (orthophosphate) and condensed inorganic forms (meta-,
pyro- and other polyphosphates) of phosphates present in the sample.
225
PHOSPHORUS, TOTAL LOW RANGE (16mm VIAL)
8.69. PHOSPHORUS, TOTAL LOW RANGE (16 mm VIAL)
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 1.15 mg/L (as P)
0.01 mg/L
±0.05 mg/L or ±6% of reading at 25 °C, whichever is greater
LED with narrow band interference filter @ 610 nm
Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93758V-0*
Phosphorus Reagent Vial
1 vial
HI93758C-0
NaOH solution 1.54N
2 mL
HI93758-0
Phosphorous Reagent 1 packet
PERFULFATE/P
Potassium Persulfate
1 packet
* Reagent vial Identification: P TLR, red label
Note: Store the unused vials in their container in a cool and dark place.
REAGENT SETS
HI93758C-50
Reagents for 50 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions and notes. Failure to
do so may result in serious injury to the operator.
• Preheat the HANNA® Reactor HI839800 to 150 °C (302°F). The optional HI740217 safety
shield is strongly recommended.
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and
possibly explosive atmosphere.
226
• Add 5.0 mL of sample to the vial, while keeping the vial at
a 45-degree angle.
• Add one packet of PERSULFATE/P Potassium Persulfate. Replace the cap and shake gently the
vial until all the powder is completely dissolved.
• Insert the vial into the reactor and heat it for 30 minutes at 150°C.
PHOSPHORUS, TOTAL LOW RANGE (16 mm VIAL)
• Remove the cap from a HI93758V-0 Reagent Vial.
• At the end of the digestion place the vials carefully in the test tube
rack and allow to cool to room temperature.
WARNING: the vials are still hot, use caution when handling.
• Select the Phosphorus Total LR (16) method using the procedure
described in the Method Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in the
Using the 16 mm Vial Adapter section (see page 22).
• Remove the cap from the vial and add exactly 2.0 mL
of HI93758C-0 NaOH Solution 1.54 N, while keeping
the vial at a 45-degree angle.
• Replace the cap and invert the vial several times to mix.
227
PHOSPHORUS, TOTAL LOW RANGE (16mm VIAL)
• Place the vial into the holder.
• Press Zero key. The display will show"-0.0-" when the meter is zeroed and ready for
measurement.
• Remove the vial.
• Remove the cap and add one packet of HI93758-0 Phosphorus
Reagent.
• Replace the cap and shake for 2 minutes until the powder is
completely dissolved.
• Place the vial into the holder.
• Press Timer and the display will show the countdown prior to the measurement, or alternatively,
wait for 3 minutes and press Read. The instrument displays the results in mg/L of phosphorus
(P).
228
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Arsenate at any level
Silica above 50 mg/L
Sulfide above 90 mg/L.
Turbidity and suspended matter in large amounts may cause interference because the strongly
acidic reaction conditions may dissolve suspended matter or cause desorption of phosphates from
particles. Before measurement, turbidity or suspended matter should be removed by treatment with
active carbon and by prior filtration.
PHOSPHORUS, TOTAL LOW RANGE (16 mm VIAL)
Note: The method detects free (orthophosphate) and condensed inorganic forms
(meta-, pyro- and other polyphosphates) of phosphates present in the sample.
• Press  or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of phosphate (PO43¯) and phosphorus
pentoxide (P2O5).
229
PHOSPHORUS, TOTAL HIGH RANGE (16 mm VIAL)
8.70. PHOSPHORUS, TOTAL HIGH RANGE (16 mm VIAL)
SPECIFICATIONS
Range
0.0 to 32.6 mg/L (as P)
Resolution
0.1 mg/L
Accuracy
±0.5 mg/L or ±5% of reading at 25 °C, whichever is greater
Light Source
LED with narrow band interference filter @ 420 nm
Method
Adaptation of Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P C, vanadomolybdophosphoric acid method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93758V-0HR*
Phosphorus Reagent Vial
2 vials
HI93758C-0
NaOH solution 1.54N
4 mL
HI93763B-0
Total Phosphorous High Range Reagent B 1 mL
DEIONIZED120
Deionized Water
5 mL
PERSULFATE/P
Potassium Persulfate
2 packets
*Reagent vial Identification: P THR, green label
Note: Store the unused vials in their container in a cool and dark place.
REAGENT SETS
HI93763B-50
Reagents for up to 49 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
Before using the reagent kit carefully read all the instructions and the Safety Data
Sheets (SDS). Pay particular attention to all warnings, cautions and notes. Failure
to do so may result in serious injury to the operator.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may
be used more than once. The blank vial is stable for one day at room temperature.
• Preheat the HANNA® Reactor HI839800 to 150 °C (302°F). The optional HI740217 safety
shield is strongly recommended.
DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and
possibly explosive atmosphere.
230
• Add 5 mL of deionized water to the first vial (#1) and 5 mL of sample to the second vial (#2),
while keeping the vials at a 45-degree angle.
• Add one packet of PERFSULFATE/P Potassium Persulfate to each vial. Replace the cap and shake
gently until all the powder is completely dissolved.
PHOSPHORUS, TOTAL HIGH RANGE (16 mm VIAL)
• Remove the cap from two HI93758V-OHR Phosphorus Reagent Vials.
• Insert the vials into the reactor and heat them for 30 minutes at 150°C.
• At the end of the digestion place the vials carefully in the test tube
rack and allow to cool to room temperature.
WARNING: The vials are still hot, use caution when handling.
231
PHOSPHORUS, TOTAL HIGH RANGE (16 mm VIAL)
• Select the Phosphorus Total HR (16) method using the procedure described in the Method
Selection section (see page 19).
• Insert the 16 mm vial adapter using the procedure described in the Using the 16 mm Vial
Adapter section (see page 22).
• Remove the cap from the vials and add 2.0 mL of HI93758C-0 NaOH Solution 1.54N to each
vial, while keeping the vials at a 45-degree angle. Replace the cap tightly and invert the vials
several times to mix.
• Remove the cap from the vials and add 0.5 mL of HI93763B-0 Total Phosphorous High Range
Reagent B to each vial, while keeping the vial at a 45-degree angle. Replace the cap and invert
several times to mix.
#1
• Place the blank vial (#1) into the holder.
• Press Timer and the display will show the countdown prior to the measurement, or alternatively
wait for 7 minutes and press Zero. The display will show "-0.0-" when the meter is zeroed and
ready for measurement.
232
• Remove the blank vial.
• Place the sample vial (#2) into the holder.
• Press Read key. The instrument displays the results in mg/L phosphorus (P).
Note: The method detects free (orthophosphate), condensed inorganic forms (meta-, pyroand other polyphosphates) and organic forms of phosphates present in the sample.
• Press  or  to access the second level functions and then press the Chem Frm key to
convert the result to mg/L of phosphate (PO43¯) and phosphorus pentoxide (P2O5).
PHOSPHORUS, TOTAL HIGH RANGE (16 mm VIAL)
#2
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Arsenate
pH: the sample should have a neutral pH
Temperature: the method is temperature sensitive.
It is recommended to add the Molybdovanadate Reagent and to run measurements at T = 20 to 25 °C:
T < 20 °C causes a negative error
T > 25 °C causes a positive error
Turbidity and suspended matter in large amounts may cause interference because the strongly
acidic reaction conditions may dissolve suspended matter or cause desorption of phosphates from
particles. Before measurement, turbidity or suspended matter should be removed by treatment with
active carbon and by prior filtration.
233
POTASSIUM
8.71. POTASSIUM
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method 0.0 to 20.0 mg/L (as K)
0.1 mg/L
±3.0 mg/L ±7% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the Turbidimetric Tetraphenylborate method
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93750A-0
Potassium Reagent A
6 drops
HI93750B-0
Potassium Reagent B
1 packet
REAGENT SETS
HI93750-01
Reagents for 100 tests
HI93750-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Potassium method using the procedure described
in the Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of sample (up to the mark).
• Add 6 drops of HI93750A-0 Potassium Reagent A.
Replace the cap and swirl the solution.
• Place the cuvette into the holder and close the lid.
234
POTASSIUM
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Add one packet of HI93750B-0 Potassium Reagent B
Replace the cap and shake gently for 1 minute.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 3 minutes.
• After waiting 3 minutes, invert again the cuvette 5 times to mix.
• Reinsert the cuvette into the instrument and close the lid.
• Press Read to start reading. The instrument displays the results in mg/L of potassium (K).
• Press  or  to access the second level functions.
235
POTASSIUM
•Press the Chem Frm key to convert the result to mg/L of potassium oxide (K2O).
•Press or  to return to the measurement screen.
INTERFERENCES
Interferences may be caused by:
Ammonium above 10 ppm
Calcium above 10000 ppm as CaCO3
Chloride above 12000 ppm
Magnesium above 8000 ppm as CaCO3
Sodium above 8000 ppm
236
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.00 to 2.00 mg/L (as SiO2)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 610 nm
Adaptation of the ASTM Manual of Water and Environmental Technology,
D859, Heteropoly Molybdenum Blue method
SILICA LOW RANGE
8.72. SILICA LOW RANGE
REQUIRED REAGENTS
CodeDescriptionQuantity
HI93705A-0
Silica Low Range Reagent A
6 drops
HI93705B-0
Silica Low Range Reagent B
1 packet
HI93705C-0
Silica Low Range Reagent C
1 packet
REAGENT SETS
Reagents for 100 tests
HI93705-01
HI93705-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Silica LR method using the procedure described
in the Method Selection section (see page 19).
• Fill the cuvette with 10 mL of unreacted sample
(up to the mark).
• Add 6 drops of HI93705A-0 Silica Low Range
Reagent A. Replace the cap and swirl the solution.
10 mL
• Press Timer and the display will show the
countdown prior to adding HI93705B-0 Silica Low
Range Reagent B, or alternatively wait 4 minutes.
• Add one packet of HI93705B-0 Silica Low Range
Reagent B and shake until it is completely dissolved.
• Press Continue and the display will show the countdown,
or alternatively wait 1 minute.
237
SILICA LOW RANGE
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Remove the cuvette.
• Add one packet of HI93705C-0 Silica Low Range
Reagent C and shake until it is completely dissolved.
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement, or
alternatively, wait 3 minutes and press Read. When the timer ends the meter will perform
the reading. The instrument displays result in mg/L of silica (SiO2).
•Press  or  to access the second level functions.
238
• Press  or  to return to the measurement screen.
SILICA LOW RANGE
• Press the Chem Frm key to convert the result to mg/L of silicon (Si).
INTERFERENCES
Interference may be caused by:
Phosphate above 60 mg/L (causes a 2% reduction in reading)
Phosphate above 75 mg/L (causes an 11% reduction in reading)
Sulfide and high concentration of iron
Eliminate color and turbidity interferences by zeroing the meter with the original water sample.
239
SILICA HIGH RANGE
8.73. SILICA HIGH RANGE
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0 to 200 mg/L (as SiO2)
1 mg/L
±1 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Adaptation of the USEPA Method 370.1 for drinking, surface and saline
waters, domestic and industrial wastes and Standard Method 4500-SiO2
REQUIRED REAGENTS
CodeDescriptionQuantity
HI96770A-0
Silica High Range Reagent A
1 packet
HI96770B-0
Silica High Range Reagent B
1 packet
HI96770C-0
Silica High Range Reagent C
1 packet
REAGENT SETS
Reagents for 100 tests
HI96770-01
HI96770-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Silica HR method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill the cuvette with 10 mL of unreacted sample (up to the mark)
and replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
240
• Add one packet of HI96770A-0 Silica High Range
Reagent A and shake until completely dissolved.
• Add one packet of HI96770B-0 Silica High Range
Reagent B. Replace the cap and shake vigorously until
completely dissolved.
SILICA HIGH RANGE
• Remove the cuvette.
• Press Timer and the display will show the countdown prior adding HI96770C-0 Silica High
Range Reagent C, or alternatively wait 10 minutes.
• Add one packet of HI96770C-0 Silica High Range
Reagent C and shake vigorously until completely
dissolved.
• Reinsert the cuvette into the instrument and close the lid.
• Press Continue and the display will show the countdown prior to the measurement, or
alternatively wait 2 minutes and press Read. When the timer ends the meter will perform the
reading. The instrument displays the results in mg/L silica (SiO2).
241
SILICA HIGH RANGE
• Press or  to access the second level functions.
• Press the Chem Frm key to convert the result to mg/L of silicon (Si).
• Press  or  to return to the measurement screen.
INTERFERENCES
Interference may be caused by:
Phosphate above 60 mg/L (causes a 2% reduction in reading)
Phosphate above 75 mg/L (causes an 11% reduction in reading)
Sulfide and high concentration of iron
Eliminate color and turbidity interferences by zeroing the meter with the original water sample.
242
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
0.000 to 1.000 mg/L (as Ag)
0.001 mg/L
±0.020 mg/L ±5% of reading at 25 °C
LED with narrow band interference filter @ 575 nm.
Adaptation of the PAN method
REQUIRED REAGENTS
Code
Description
HI93737A-0
Silver Reagent A
HI93737B-0
Silver Reagent B
HI93737C-0
Silver Reagent C
HI93737D-0
Silver Reagent D
HI93703-51
Dispersing Agent
SILVER
8.74. SILVER
Quantity
1 mL
1 mL
2 mL
2 mL
6 drops
REAGENT SETS
HI93737-01
Reagents for 50 tests
HI93737-03
Reagents for 150 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Silver method using the procedure described in the Method
Selection section (see page 19).
Note: For best results perform your tests between 20-24 °C.
• Fill two graduated beakers with 25 mL of sample.
• Add 1 mL of HI93737A-0 Silver Reagent A to beaker #1 (the
blank) and swirl gently to mix.
243
SILVER
• Add 1mL of HI93737B-0 Silver Reagent B to beaker #2
(the sample) and swirl gently to mix.
• Press Timer and the display will show the countdown prior to adding HI93737C-0 Silver Reagent
C or alternatively, wait for 2 minutes.
• Add 1 mL of HI93737C-0 Silver Reagent C to each beaker
and swirl.
•Press Continue and the display will show the countdown prior to adding HI93737D-0 Silver
Reagent D, or alternatively wait for 2 minutes.
• Add 1 mL of HI93737D-0 Silver Reagent D to each beaker
and swirl.
244
SILVER
• Press Continue and the display will show the countdown or alternatively wait for 2 minutes.
#1
• Fill cuvette (#1) up with 10 mL of the blank (up to the mark).
10 mL
• Add 3 drops of HI93703-51 Dispersing Agent, replace the
cap and invert gently for 10 seconds.
#1
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
• Fill a second cuvette (#2) up with 10 mL of the reacted sample
(up to the mark).
10 mL
#2
245
SILVER
• Add 3 drops of HI93703-51 Dispersing Agent, replace
the cap and invert gently for 10 seconds.
• Insert the second cuvette (#2) into the instrument.
#2
• Press Read to start the reading. The instrument displays the results in mg/L of silver (Ag).
INTERFERENCES
Interference may be caused by:
Al3+ above 30 mg/L
Ca2+ above 1000 mg/L (as CaCO3)
Cd2+ above 20 mg/L
Cl¯ above 8000 mg/L
Co2+ above 1.5 mg/L
Cr3+ above 20 mg/L
Cr6+ above 40 mg/L
Cu2+ above 15 mg/L
F¯ above 20 mg/L
246
Fe2+ above 1.5 mg/L
Fe3+ above 10 mg/L
K+ above 500 mg/L
Mn2+ above 25 mg/L
Mg2+ above 1000 mg/L (as CaCO3)
Na+ above 5000 mg/L
Ni2+ above 1.5 mg/L
Pb2+ above 20 mg/L
Zn2+ above 30 mg/L
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method SULFATE
8.75. SULFATE
0 to 150 mg/L (as SO42-)
1 mg/L
±5 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 466 nm
Sulfate is precipitated with barium chloride crystals
REQUIRED REAGENTS
Code
Description
HI93751-0
Sulfate Reagent
Quantity
1 packet
REAGENT SETS
HI93751-01
Reagents for 100 tests
HI93751-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Sulfate method using the procedure described in the
Method Selection section (see page 19).
10 mL
• Fill a cuvette with 10 mL of unreacted sample (up to the mark) and
replace the cap.
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for
measurement.
247
SULFATE
• Add one packet of HI93751-0 Sulfate Reagent.
• Replace the cap and invert gently for 1 minute (about
30 inversions).
• Reinsert the cuvette into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively,
wait for 5 minutes and press Read. When the timer ends the meter will perform the reading.
The instrument displays the concentration in mg/L of sulfate (SO42-).
INTERFERENCES
Interferences may be caused by:
Calcium (as CaCO3) above 20000 mg/L
Chloride (as Cl¯) above 40000 mg/L
Magnesium (as MgCO3) above 10000 mg/L
Silica (as SiO2) above 500 mg/L
Color or suspended matter in large amounts will interfere: suspended matter should be removed by
previous filtration.
Organic matter in large amounts may impede the precipitation of barium sulfate.
248
SPECIFICATIONS
Range
0.00 to 3.50 mg/L (as SDBS)
Resolution
0.01 mg/L
Accuracy
±0.04 mg/L ±3% of reading at 25 °C
Light Source
LED with narrow band interference filter @ 610 nm
Method Adaptation of the USEPA method 425.1 and Standard Methods for the
Examination of Water and Wastewater, 20th edition, 5540C, Anionic Surfactants as MBAS
REQUIRED REAGENTS
CodeDescriptionQuantity
HI95769A-0
Anionic Surfactants Reagent A 4 drops
HI95769B-0
Anionic Surfactants Reagent B 2 drops
-
Chloroform Reagent
10 mL
DEIONIZED120
Deionizate Water
15 mL
SURFACTANTS, ANIONIC
8.76. SURFACTANTS, ANIONIC
REAGENT SETS
HI95769-01
Reagents for 40 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Surfactants (Anionic) method using the procedure described in
the Method Selection section (see page 19).
• Fill the graduated glass vial with 25 mL of sample.
Note: For improved accuracy the use of class A laboratory pipettes is recommended.
• Add 2 drops of HI95769A-0 Anionic Surfactants Reagent A and 2 drops
of HI95769B-0 Anionic Surfactants Reagent B.
• Close vial with its cap and invert to mix, the solution will turn blue.
• Add 10 mL of Chloroform.
249
SURFACTANTS, ANIONIC
Note: Chloroform is more dense than water will sink to the bottom
of the graduated glass vial.
• Invert the vial twice and remove the cap to release any pressure that
has built up.
• Close the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Ensure the cap is secure when shaking.
•Press Timer and the display will show the countdown or, alternatively, wait for 2 minutes.
During this period the chloroform layer separates from the aqueous layer, the color of the
aqueous layer will fade slightly, while the chloroform layer will turn blue.
• Remove the cap.
• Remove the upper aqueous layer using the long plastic pipette, do not
remove the lower chloroform layer.
• Add 15 mL of deionized water to the vial (up to the 25 mL mark).
• Add 2 drops of HI95769A-0 Anionic Surfactants Reagent A.
• Invert the vial twice and remove the cap to release any pressure that
has built up.
• Close the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Ensure the cap is secure when shaking.
250
• Remove the cap.
• Insert a clean plastic pipette below the upper aqueous layer to transfer
the lower chloroform layer into a cuvette. Do not transfer any of the upper
aqueous layer.
SURFACTANTS, ANIONIC
• Press Continue and the display will show the countdown, or alternatively, wait for 2 minutes.
During this period, the chloroform layer separates from the aqueous layer.
Notes: The solution in the cuvette must be clear. If the solution is cloudy, the separation
between the chloroform and aqueous layer can be improved by gently warming the cuvette
(holding the vial in your hand). If the chloroform layer contains some aqueous drops
hanging on the cuvette wall, gently swirl or invert the cuvette.
It is important to transfer at least 7 mL of chloroform layer into the measurement cuvette,
thus up to 0.5 cm (1/4”) below the 10 mL mark. If the transferred volume is lower than 7
mL, the accuracy of the test may be affected. Please repeat the test waiting for longer than
2 minutes to allow complete separation between the two phases.
• Cap the cuvette. This is the reacted sample (#2).
#2
#1 blank
• Fill another cuvette with 10 mL of Chloroform reagent (up to the
mark) and place the cap. This is the blank (#1).
#1
• Place the blank (cuvette #1) into the holder and close the lid.
251
SURFACTANTS, ANIONIC
• Press Zero the display, depending on the measurement phase. After a few seconds, the display
will show “-0.0-” when the meter is zeroed and ready for measurement.
• Remove the cuvette.
#2
• Place the reacted sample (#2) into the instrument and close the lid.
• Press Read key to start the reading. The instrument displays the result in mg/L as SDBS.
INTERFERENCES
Interferences may be caused by:
Cationic surfactants negative interference
Absorption particulate matter negative interference
Sulfide negative interference
Organic sulfates, sulfonates positive interference
Strong oxidants (Cl2, H2O2, S2O82-, etc.) negative interference
Highly buffered samples or with extreme pH may exceed the buffering capacity of the reagent: pH
should be adjusted between 4 and 9 with diluted NaOH for acidic samples or with diluted HCl for
alkaline samples, prior to addition of the reagent.
252
SPECIFICATIONS
Range
Resolution
Accuracy
Light Source
Method
ZINC
8.77. ZINC
0.00 to 3.00 mg/L (as Zn)
0.01 mg/L
±0.03 mg/L ±3% of reading at 25 °C
LED with narrow band interference filter @ 575 nm
Adaptation of Standard Methods for the Examination of Water and Wastewater, 18th edition, Zincon method
REQUIRED REAGENT
Code
Description
HI93731A-0
Zinc Reagent A
HI93731B-0
Zinc Reagent B
Quantity
1 packet
0.5 mL
REAGENT SETS
Reagents for 100 tests
HI93731-01
HI93731-03
Reagents for 300 tests
For other accessories see page 267.
MEASUREMENT PROCEDURE
• Select the Zinc method using the procedure described in the Method
Selection section (see page 19).
• Fill the graduated glass vial up to the 20 mL mark with the sample.
20 mL
• Add one packet of HI93731A-0 Zinc Reagent A, close the
cylinder, and invert several times to mix until completely
dissolved.
• Fill a cuvette with 10 mL of the reacted sample (up to the
mark) and close the cap.
10 mL
253
ZINC
• Place the cuvette into the holder and close the lid.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready
for measurement.
• Add 0.5 mL of HI93731B-0 Zinc Reagent B to the cuvette,
close the cuvette with the supplied HDPE plastic stopper to
prevent contamination.
• Replace the cap and mix for 15 seconds.
• Insert the sample into the instrument and close the lid.
• Press Timer and the display will show the countdown prior to the measurement or,
alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the
meter will perform the reading. The instrument displays the results in mg/L of zinc (Zn).
254
ZINC
INTERFERENCES
Interference may be caused by:
Aluminum above 6 mg/L Iron above 7 mg/L
Cadmium above 0.5 mg/L Manganese above 5 mg/L
Copper above 5 mg/L Nickel above 5 mg/L
255
WARNINGS & ERRORS
9. WARNINGS & ERRORS
The instrument shows clear warning messages when erroneous conditions appear and when measured
values are outside the expected range. The information below provides an explanation of the errors
and warnings, and recommended action to be taken.
Explanation: There is an excess amount of ambient light reaching the
detector.
Recommended action: Make sure the lid is closed before performing
any measurements. If the issue persists, please contact Hanna
Instruments technical support.
Explanation: The sample and the Zero cuvettes are inverted.
Recommended action: Swap the cuvettes and repeat the measurement.
Explanation: There is either too much light or the instrument can not
adjust the light level.
Recommended action: Please check the preparation of the Zero
cuvette and that the sample does not contain any debris.
Explanation: The meter is either overheating or its temperature has
dropped too low to operate within published accuracy specifications.
Recommended action: Allow the meter to reach normal environmental
temperature before performing any measurements.
Explanation: Meter temperature has changed significantly since the
zero measurement has been performed.
Recommended action: The zero measurement must be performed
again.
Explanation: The measured value is outside the limits of the method.
Recommended action: If possible, change the method range. Verify
that the sample does not contain any debris. Check the sample
preparation and the measurement preparation.
256
Explanation: Stored results of the CAL Check measurements have been
lost.
Recommended action: Please redo the CAL Check measurements to
ensure accurate results.
WARNINGS & ERRORS
Explanation: The measured value cannot be calculated.
Recommended action: Please check sample preparation and
measurement procedure.
Explanation: User settings have been lost.
Recommended action: Please reset the values. If the issue persists,
please contact Hanna Instruments technical support.
Explanation: Flash drive is not recognized or it might be damaged.
Recommended action: Please insert a new USB flash drive.
Explanation: Data log is full.
Recommended action: Please review logged data and delete
unnecessary logs.
Explanation: Date and time settings have been lost.
Recommended action: Please reset the values. If the issue persists,
please contact Hanna Instruments technical support.
Explanation: Battery level is too low to ensure normal functioning and
the meter will turn off.
Recommended action: Connect the USB adapter to charge the battery.
257
WARNINGS & ERRORS
The instrument shows warning messages when some of the features become unavailable. To recover
them follow the Recommended action: Restart the meter. If the issue persists, please contact Hanna
Instruments technical support.
Explanation: English is the only available language. Help function is
not available.
Explanation: Real time clock it's not accurate.
Explanation: The device serial number inside the memory can not be
identified.
Explanation: Logged data are no longer accessible.
Explanation: Battery charge level is not accurate.
If a critical error appears, below message is displayed.
Explanation: A critical error has occured.
Recommended action: Restart the meter. If the issue persists, please
contact Hanna Instruments technical support.
258
Range
Method
Alkalinity 0 to 500 mg/L
Colorimetric
Alkalinity, Marine
0 to 300 mg/L
Colorimetric
Aluminum
0.00 to 1.00 mg/L
Aluminon
Ammonia LR
0.00 to 3.00 mg/L
Nessler
Ammonia LR (16 mm Vial)
0.00 to 3.00 mg/L
Nessler
Ammonia MR
0.00 to 10.00 mg/L Nessler
Ammonia HR
0.0 to 100.0 mg/L
Nessler
Ammonia HR (16 mm Vial)
0.0 to 100.0 mg/L
Nessler
Bromine
0.00 to 8.00 mg/L
DPD
Calcium
0 to 400 mg/L
Oxalate
Calcium Marine
200 to 600 mg/L
Zincon
Chloride
0.0 to 20.0 mg/L
Mercury(II) Thiocyanate
Chlorine Dioxide
0.00 to 2.00 mg/L
Chlorophenol Red
Chlorine Dioxide, Rapid Method
0.00 to 2.00 mg/L
DPD
Chlorine, Free LR
0.00 to 5.00 mg/L
DPD
Chlorine, Free ULR
0.000 to 0.500 mg/L DPD
Chlorine, Total LR
0.00 to 5.00 mg/L
Chlorine, Total ULR
0.000 to 0.500 mg/L DPD
Chlorine, Total UHR
0 to 500 mg/L
Standard Methods 4500-Cl
Chromium(VI) LR
0 to 300 µg/L
Diphenylcarbohydrazide
Chromium(VI) HR
0 to 1000 µg/L
Diphenylcarbohydrazide
Chemical Oxygen Demand LR (16 mm Vial)
0 to 150 mg/L
EPA 410.4
Chemical Oxygen Demand MR (16 mm Vial)
0 to 1500 mg/L
EPA 410.4
Chemical Oxygen Demand HR (16 mm Vial)
0 to 15000 mg/L
EPA 410.4
Color of Water
0 to 500 PCU
Colorimetric Platinum Cobalt
Copper LR
0.000 to 1.500 mg/L Bicinchoninate
Copper HR
0.00 to 5.00 mg/L
STANDARD METHODS
10. STANDARD METHODS
Description
DPD
Bicinchoninate
259
STANDARD METHODS
260
Description Range
Method
Cyanuric Acid 0 to 80 mg/L
Turbidimetric
Fluoride LR 0.00 to 2.00 mg/L
SPADNS
Fluoride HR
0.0 to 20.0 mg/L
SPADNS
Hardness, Calcium
0.00 to 2.70 mg/L
Calmagite
Hardness, Magnesium 0.00 to 2.00 mg/L
EDTA
Hardness, Total LR
0 to 250 mg/L
EPA 130.1
Hardness, Total MR
200 to 500 mg/L
EPA 130.1
Hardness, Total HR
400 to 750 mg/L
EPA 130.1
Hydrazine
0 to 400 µg/L
p-Dimethylaminobenzaldehyde
Iodine
0.0 to 12.5 mg/L
DPD
Iron LR
0.000 to 1.600 mg/L TPTZ
Iron HR
0.00 to 5.00 mg/L
Phenanthroline
Iron(II)
0.00 to 6.00 mg/L
EPA 315B
Iron(II)/(III)
0.00 to 6.00 mg/L
EPA 315B
Iron, Total (16 mm Vial)
0.00 to 7.00 mg/L
EPA 315B
Magnesium
0 to 150 mg/L
Calmagite
Manganesse LR
0 to 300 µg/L
PAN
Manganesse HR
0.0 to 20.0 mg/L
Periodate
Molybdenum
0.0 to 40.0 mg/L
Mercaptoacetic Acid
Nickel LR
0.000 to 1.000 mg/L PAN
Nickel HR
0.00 to 7.00 g/L
Colorimetric
Nitrate 0.0 to 30.0 mg/L
Cadmium reduction
Nitrate (16 mm Vial)
0.0 to 30.0 mg/L
Chromotropic Acid
Nitrite, Marine ULR
0 to 200 µg/L
Diazotization
Nitrite LR
0 to 600 µg/L
Diazotization
Nitrite HR
0 to 150 mg/L
Ferrous Sulfate
Nitrogen, Total LR (16 mm Vial)
0.0 to 25.0 mg/L
Chromotropic Acid
Method
Nitrogen, Total HR (16 mm Vial)
10 to 150 mg/L
Chromotropic Acid
Oxygen, Dissolved 0.0 to 10.0 mg/L
Winkler
Oxygen Scavengers (Carbohydrazide)
0.00-1.50mg/L
Iron Reduction
Oxygen Scavengers (DEHA) 0 to 1000 µg/L
Iron Reduction
Oxygen Scavengers (Hydroquinone)
0.00-2.50mg/L
Iron Reduction
Oxygen Scavengers (Iso-Ascorbic Acid)
0.00-4.50mg/L
Iron Reduction
Ozone
0.00 to 2.00 mg/L
DPD
pH
6.5 to 8.5 pH
Phenol Red
Phosphate, Marine ULR
0 to 200 µg/L
Ascorbic Acid
Phosphate LR
0.00 to 2.50 mg/L
Ascorbic Acid
Phosphate HR
0.0 to 30.0 mg/L
Amino Acid
Phosphorus, Reactive LR (16 mm Vial)
0.00 to 1.60 mg/L
Ascorbic Acid
Phosphorus, Reactive HR (16 mm Vial)
0.0 to 32.6 mg/L
Vanadomolybdophosphoric Acid
Phosphorus, Acid Hydrolyzable (16mm Vial) 0.00 to 1.60 mg/L
Ascorbic Acid
Phosphorus, Total LR (16 mm Vial)
0.00 to 1.15 mg/L
Ascorbic Acid
Phosphorus, Total HR (16 mm Vial)
0.0 to 32.6 mg/L
Vanadomolybdophosphoric Acid
Potassium 0.0 to 20.0 mg/L
Tetraphenylborate
Silica LR
0.00 to 2.00 mg/L
Heteropoly Blue
Silica HR
0 to 200 mg/L
EPA
Silver 0.000 to 1.000 mg/L PAN
Sulfate 0 to 150 mg/L
Barium Chloride
Surfactants, Anionic 0.00 to 3.50 mg/L
EPA 425.1
Zinc
0.00 to 3.00 mg/L
Zincon
STANDARD METHODS
Description Range
261
ACCESSORIES
262
11. ACCESSORIES
11.1. REAGENT SETS
Description
Code
HI736-25
25 phosphate marine ULR tests
HI755-26
25 alkalinity marine tests
HI758-26
25 calcium marine tests
HI764-25
25 nitrite marine ULR tests
HI775-26
25 alkalinity fresh water tests
HI93700-01
100 ammonia LR tests
HI93700-03
300 ammonia LR tests
HI93701-01
100 chlorine free tests (powder)
HI93701-03
300 chlorine free tests (powder)
HI93701-F
300 chlorine free tests (liquid)
HI93701-T
300 chlorine total tests (liquid)
HI93702-01
100 copper HR tests
HI93702-03
300 copper HR tests
HI93703-52
100 ozone tests
HI93704-01
100 hydrazine tests
HI93704-03
300 hydrazine tests
HI93705-01
100 silica LR tests
HI93705-03
300 silica LR tests
HI93707-01
100 nitrite LR tests
HI93707-03
300 nitrite LR tests
HI93708-01
100 nitrite HR tests
HI93708-03
300 nitrite HR tests
HI93709-01
100 manganese HR tests
HI93709-03
300 manganese HR tests
HI93710-01
100 pH tests
Description
300 pH tests
HI93711-01
100 chlorine total tests (powder)
HI93711-03
300 chlorine total tests (powder)
HI93712-01
100 aluminum tests
HI93712-03
300 aluminum tests
HI93713-01
100 phosphate LR tests
HI93713-03
300 phosphate LR tests
HI93715-01
100 ammonia MR tests
HI93715-03
300 ammonia MR tests
HI93716-01
100 bromine tests
HI93716-03
300 bromine tests
HI93717-01
100 phosphate HR tests
HI93717-03
300 phosphate HR tests
HI93718-01
100 iodine tests
HI93718-03
300 iodine tests
HI93719-01
100 hardness magnesium tests
HI93719-03
300 hardness magnesium tests
HI93720-01
100 hardness calcium tests
HI93720-03
300 hardness calcium tests
HI93721-01
100 iron HR tests
HI93721-03
300 iron HR tests
HI93722-01
100 cyanuric acid tests
HI93722-03
300 cyanuric acid tests
HI93723-01
100 chromium(VI) HR tests
HI93723-03
300 chromium(VI) HR tests
HI93726-01
100 nickel HR tests
HI93726-03
300 nickel HR tests
ACCESSORIES
Code
HI93710-03
263
ACCESSORIES
264
Code
HI93728-01
Description
100 nitrate tests
HI93728-03
300 nitrate tests
HI93729-01
100 fluoride LR tests
HI93729-03
300 fluoride LR tests
HI93730-01
100 molybdenum tests
HI93730-03
300 molybdenum tests
HI93732-01
100 dissolved oxygen tests
HI93732-03
300 dissolved oxygen tests
HI93731-01
100 zinc tests
HI93731-03
300 zinc tests
HI93733-01
100 ammonia HR tests
HI93733-03
300 ammonia HR tests
HI93735-01
100 hardness total MR tests (200 to 500 mg/L)
HI93735-02
100 hardness total HR tests (400 to 750 mg/L)
HI93735-0
300 hardness total tests (LR - 100 tests, MR - 100 tests, HR - 100 tests)
HI93735-00
100 hardness total LR tests (0 to 250 mg/L)
HI93737-01
50 silver tests
HI93737-03
150 silver tests
HI93738-01
100 chlorine dioxide tests
HI93738-03
300 chlorine dioxide tests
HI93739-01
100 fluoride HR tests
HI93739-03
300 fluoride HR tests
HI93740-01
50 nickel LR tests
HI93740-03
150 nickel LR tests
HI93746-01
50 iron LR tests
HI93746-03
150 iron LR tests
HI93748-01
50 manganese LR tests
Description
150 manganese LR tests
HI93749-01
100 chromium(VI) LR tests
HI93749-03
300 chromium(VI) LR tests
HI93750-01
100 potassium tests
HI93750-03
300 potassium tests
HI93751-01
100 sulfate tests
HI93751-03
300 sulfate tests
HI937520-01
50 magnesium tests
HI937520-03
150 magnesium tests
HI937521-01
50 calcium fresh water tests
HI937521-03
150 calcium fresh water tests
HI93753-01
100 chloride tests
HI93753-03
300 chloride tests
HI93754A-25
24 chemical oxygen demand LR tests (Viol)
HI93754B-25
24 chemical oxygen demand MR tests (Vial)
HI93754C-25
24 chemical oxygen demand HR tests (Vial)
HI93757-01
100 ozone tests
HI93757-03
300 ozone tests
HI93758A-50
50 phosphorus reactive LR tests (Vial)
HI93758B-50
50 phosphorus acid hydrolyzed tests (Vial)
HI93758C-50
50 phosphorus total LR tests (Vial)
HI93763A-50
49 phosphorus reactive HR tests (Vial)
HI93763B-50
49 phosphorus total HR tests (Vial)
HI93764A-25
25 ammonia LR tests (vial)
HI93764B-25
25 ammonia HR tests (vial)
HI93766-50
50 nitrate tests (Vial)
ACCESSORIES
Code
HI93748-03
265
ACCESSORIES
266
Code
HI93767A-50
Description
49 nitrogen total LR tests (Vial)
HI93767B-50
49 nitrogen total HR tests (Vial)
HI95747-01
100 copper LR tests
HI95747-03
300 copper LR tests
HI95761-01
100 chlorine total ULR tests
HI95761-03
300 chlorine total ULR tests
HI95762-01
100 chlorine free ULR tests
HI95762-03
300 chlorine free ULR tests
HI95769-01
40 surfactants anionic tests
HI96770-01
100 silica HR tests
HI96770-03
300 silica HR tests
HI95771-01
100 chlorine total UHR tests
HI95771-03
300 chlorine total UHR tests
HI96773-01
50 oxygen scavengers tests
HI96773-03
150 oxygen scavengers tests
HI96776-01
100 iron(II) tests
HI96776-03
300 iron(II) tests
HI96777-01
100 iron(II)/(III) tests
HI96777-03
300 iron(II)/(III) tests
HI96778-25
25 total iron tests (Vial)
HI96779-01
100 chlorine dioxide (rapid) tests
HI96779-03
300 chlorine dioxide (rapid) tests
Description
Triple ceramic, double junction, low temperature glass, refillable pH
electrode with conical tip and temperature sensor
Triple ceramic, double junction, high temperature glass, refillable pH
electrode with temperature sensor
Glass body, double junction, refillable pH/temperature electrode
Glass body, double junction, refillable pH/temperature electrode with
enhanced diagnostics
Plastic body, double junction, gel filled, non refillable pH/temperature
electrode
Plastic body, double junction, gel filled, non refillable pH/temperature
electrode with enhanced diagnostics
Glass body, double junction with temperature sensor for wine analysis
Double junction, open reference, non refillable, electrolyte viscolene, PVDF
body with conical tip, pH/temperature electrode
Double junction, open reference, non refillable, electrolyte viscolene, glass
body with conical tip, pH/temperature electrode
Double junction, open reference, non refillable, electrolyte viscolene, PVDF
body with conical tip, pH/temperature electrode
ACCESSORIES
11.2 pH ELECTRODES
Code
HI10530
HI10430
HI11310
HI11311
HI12300
HI12301
HI10480
FC2320
FC2100
FC2020
Note: The enhanced diagnostics information are not displayed by meter.
267
ACCESSORIES
11.3 pH SOLUTIONS
BUFFER SOLUTIONS
Code
HI70004P
HI70007P
HI70010P
HI7001L
HI7004L
HI7006L
HI7007L
HI7009L
HI7010L
HI8004L
HI8006L
HI8007L
HI8009L
HI8010L
Description
pH 4.01 Buffer Sachets, 20 mL (25 pcs.)
pH 7.01 Buffer Sachets, 20 mL (25 pcs.)
pH 10.01 Buffer Sachets, 20 mL (25 pcs.)
pH 1.68 Buffer Solution, 500 mL
pH 4.01 Buffer Solution, 500 mL
pH 6.86 Buffer Solution, 500 mL
pH 7.01 Buffer Solution, 500 mL
pH 9.18 Buffer Solution, 500 mL
pH 10.01 Buffer Solution, 500 mL
pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
ELECTRODE STORAGE SOLUTIONS
HI70300L
Storage Solution, 500 mL
HI80300L
Storage Solution in FDA approved bottle, 500 mL
ELECTRODE CLEANING SOLUTIONS
HI70000P
Electrode Rinse Sachets, 20 mL (25 pcs.)
HI7061L
General Cleaning Solution, 500 mL
HI7073L
Protein Cleaning Solution, 500 mL
HI7074L
Inorganic Cleaning Solution, 500 mL
HI7077L
Oil & Fat Cleaning Solution, 500 mL
HI8061L
General Cleaning Solution in FDA approved bottle, 500 mL
HI8073L
Protein Cleaning Solution in FDA approved bottle, 500 mL
HI8077L
Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL
ELECTRODE REFILL ELECTROLYTE SOLUTIONS
HI7082
3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes
HI8082
3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction
electrodes.
268
ACCESSORIES
11.4. OTHER ACCESSORIES
Code
Description
carrying case
HI72083300
HI731311
vial cuvette 16 mm diam (5 pcs.)
HI731318
cloth for wiping cuvettes (4 pcs.)
glass cuvettes (4 pcs.)
HI731331
HI731335N
cap for cuvette (4 pcs.)
HI731340
200 µL automatic pipette
HI731341
1000 µL automatic pipette
HI731342
2000 µL automatic pipette
cap for 100 mL beaker (10 pcs.)
HI740034P
HI740036P
100 mL plastic beaker (10 pcs.)
HI740038
60 mL glass bottle and stopper
HI740142P
1 mL graduated syringe (10 pcs)
HI740143
1 mL graduated syringe (6 pcs.)
pipette tip (6 pcs.)
HI740144
HI740157P
plastic refilling pipette (20 pcs.)
HI740216
Cooling Rack
HI740217
safety shield for reactor
HI740220
25 mL graduated glass vial (2 pcs.)
170 mL plastic beaker
HI740223
HI740224
170 mL plastic beaker (12 pcs.)
HI740225
60 mL graduated syringe
HI740226
5 mL graduated syringe
filter assembly
HI740227
HI740228
filter discs (25 pcs.)
HI740229
100 mL graduated cylinder
HI74083300
COD Adapter
DEMI-02
demineralizer
USB power adapter, European plug
HI75110/220E
HI75110/220U
USB power adapter, USA plug
HI76404A
electrode holder
HI83399-11
CAL Check cuvette kit for HI83399
HI83300-100
Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with
cap, 170 mL graduated beaker with cap, 3 mL pipette, 60 mL syringe,
5 mL syringe, graduated cylinder, spoon, funnel, filtter paper (25 pcs.).
269
ACCESSORIES
270
Code
HI839800-01
HI839800-02
HI920015
HI93703-50
HI93703-55
Description
reactor, European plug
reactor, USA plug
USB to micro USB cable connector
cuvette cleaning solution (230 mL)
activated carbon (50 pcs.)
ABBREVIATIONS
12. ABBREVIATIONS
EPA:
US Environmental Protection Agency
°C:
degree Celsius
°F:
degree Fahrenheit
µg/L: micrograms per liter (ppb)
mg/L: milligrams per liter (ppm)
g/L:
grams per liter (ppt)
mL:
milliliter
GLP
good laboratory practice
UHR
ultra high range
ULR
ultra low range
HR:
high range
MR:
medium range
LR:
low range
PAN:
1-(2-pyridylazo)-2-naphtol
TPTZ: 2,4,6-tri-(2-pyridyl)-1,3,5-triazine
271
CERTIFICATION
All Hanna Instruments conform to the CE European Directives.
RoHS
compliant
Disposal of Electrical & Electronic Equipment. The product should not be treated as household
waste. Instead hand it over to the appropriate collection point for the recycling of electrical and
electronic equipment which will conserve natural resources.
Disposal of waste batteries. This product contains batteries, do not dispose of them with other
household waste. Hand them over to the appropriate collection point for recycling.
Ensuring proper product and battery disposal prevents potential negative consequences for the
environment and human health. For more information, contact your city, your local household
waste disposal service, the place of purchase or go to www.hannainst.com.
MAN83399 05/19
272
Recommendations
for Users
Warranty
Before using this product, make sure it is entirely suitable for your
specific application and for the environment in which it is used.
Any variation introduced by the user to the supplied equipment
may degrade the meters’ performance. For yours and the meter’s
safety do not use or store the meter in hazardous environments.
The HI83399 is warranted for two years against defects in workmanship and materials
when used for their intended purpose and maintained according to instructions.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is
not covered.
If service is required, contact your local Hanna Instruments Office. If under warranty,
report the model number, date of purchase, serial number and the nature of the
problem. If the repair is not covered by the warranty, you will be notified of the
charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain
a Returned Goods Authorization (RGA) number from the Technical Service department
and then send it with shipping costs prepaid. When shipping any instrument, make
sure it is properly packed for complete protection.
Hanna Instruments reserves the right to modify the design, construction or appearance of its products
without advance notice.
World
Headquarters
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com
MAN83399
Printed in ROMANIA