Eppendorf Galaxy 48R Operating Manual

New Brunswick™ Galaxy ® 48 R

Operating manual

Copyright© 2019 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner.

Eppendorf ® and the Eppendorf Brand Design are registered trademarks of Eppendorf AG, Germany.

New Brunswick™ is a trademark of Eppendorf AG, Germany.

Galaxy ® is a registered trademark of Eppendorf, Inc., USA.

VisioNize ® is a registered trademark of Eppendorf AG, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

The software of this product contains open source software. License information is available in the delivery box.

6705 900.041-02/102019



Table of contents


English (EN)

3

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1

Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2

Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1

Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2

Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3

Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1

Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2

User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3

Personal protective equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4

Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.5

Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.5.1

Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.6

Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.6.1

Description of ATEX Guideline (94/9EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.7

Warning signs on the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.1

Product overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.1.1

Inside the chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.2

Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.3

Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3.1

Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3.2

Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.4

Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.1

Operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.2

Direct heating system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.3

Infrared sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.4

Water tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.5

Seamless chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.6

Standard features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4.7

Multiple options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.4.8

Two-level alarm system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.5

Stacking devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.6

Connectivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.1

Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.2

Utilities requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3

Initial setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3.1

Installing the feet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3.2

Install the power cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.3

Making connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.4

Replaceing the shelf racks and shelves, and level the incubator . . . . . . . . . . . . . . . . . 25

4.3.5

Replacing the water tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

4.3.6

Replacing the Auto-zero filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

4

Table of contents


English (EN)

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.1

Preparing for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.2

Using the water tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.3

Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.3.1

Programming CO

2

, O

2

and temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.3.2

User access code. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.3.3

Removing user access code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.4

Referencing the CO

2

sensor with autozero . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.5

USER SETTINGS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.5.1

SET DATE AND TIME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.5.2

AUDIBLE ALARM VOLUME ADJUST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.5.3

PROGRAMMABLE CO

2

AUTOZERO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.5.4

DATALOGGER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.5.5

POWER FREQUENCY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.5.6

DISABLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.5.7

DISINFECTION (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.6

DATALOGGER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.6.1

ALARM EVENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.6.2

TEMPERATURE GRAPH + DOOR OPEN BAR CHART . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.6.3

CO

2

GRAPH + DOOR OPEN BAR CHART . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.6.4

DIAGNOSTIC CHAMBER ELEMENT GRAPH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.6.5

DIAGNOSTIC DOOR GRAPH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.6.6

DIAGNOSTIC DOOR ELEMENT GRAPH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.6.7

RESTART GRAPHIC RECORD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.7

CHAMBER ALARMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.7.1

Arm chamber alarms after selectable delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.7.2

Arm chamber alarms after setpoints are achieved . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.7.3

Door open alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.7.4

Audible and visual alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.7.5

Chamber alarm system function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.8

DIAGNOSTICS menu screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.9

HELP MENU screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.10

High-temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.10.1

Using the high-temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.10.2

High-temperature disinfection option with oxygen control . . . . . . . . . . . . . . . . . . . . . 44

5.11

Oxygen sensor removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

5.12

Alarm relay for Building Management System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

5.13

O

2

control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.13.1

Setting up the N

2

tank. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.13.2

Setting up oxygen control (0.1–19 %) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.13.3

Setting up oxygen control (1–19 %). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.14

Referencing to atmosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.15

Oxygen sensor replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.15.1

Replace sensor soon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.15.2

Replace sensor now . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.15.3

Removing and replacing O

2

sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.16

Replacing the filter disc . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

5.17

Humidity alert and monitoring package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

5.17.1

Water tray warning system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

5.17.2

Humidity display and alarm system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Table of contents


English (EN)

5

5.18

RS-232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.1

General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.2

O

2

sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1

Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1.1

General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1.2

Daily checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1.3

Weekly checks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1.4

Monthly checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1.5

CO

2

sampling with analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

7.2

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

7.3

Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.4

High-temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.5

Shipping the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

8.1

Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

8.2

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

8.3

Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.1

Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.1.1

Equipment dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.1.2

Internal dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.1.3

Transporting dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.1.4

Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9.2

Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

9.3

Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

9.4

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

9.4.1

Temperature management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

9.4.2

CO

2

control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

9.4.3

Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

9.4.4

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

9.5

Oxygen sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

10.1

Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

10.2

Available options for device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

10.3

Connectivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6

Table of contents


English (EN)

Operating instructions


English (EN)

7

1

1.1


Using this manual

 Read this operating manual completely before using the device for the first time. Observe the instructions for use of the accessories where applicable.

 This operating manual is part of the product. Please keep it in a place that is easily accessible.

 Enclose this operating manual when transferring the device to third parties.

 The current version of the operating manual for all available languages can be found on our webpage www.eppendorf.com/manuals.

1.2

Danger symbols and danger levels

The safety instructions of this operating manual indicate the following danger symbols and danger levels:

1.2.1

Danger symbols

Hazard point Burns

Electric shock Material damage

Explosion

Inhalation

Heavy loads

Crush

1.2.2

Danger levels

DANGER

WARNING

CAUTION

NOTICE

Will lead to severe injuries or death.

May lead to severe injuries or death.

May lead to light to moderate injuries.

May lead to material damage.

8



English (EN)

1.3

Symbols used

Depiction Meaning

1.

2.



Actions in the specified order

Actions without a specified order

• List

Text Display or software texts

Additional information

Safety


English (EN)

9

2

2.1

Safety

Intended use

The CO

2

incubator New Brunswick™ Galaxy ® 48 R is designed to and serve to provide stable and homogeneous atmosphere required for cell culture by controlling temperature, carbon dioxide and optionally oxygen for cultivating samples and cells from biological laboratories. The incubator is intended exclusively for use indoors and for operation by qualified staff for research purposes only.

2.2

User profile

The device and accessories may only be operated by trained and skilled personnel.

Before using the device, read the operating manual and the instructions for use of the accessories carefully and familiarize yourself with the device's mode of operation.

2.3

Personal protective equipment

Personal protective equipment protects your life and your health.

 Always wear the personal protective equipment required for the biosafety level and by the laboratory regulations.

 Always wear protective clothing, protective gloves, and safety boots.

 If additional protective equipment is required, this is indicated above the respective instruction.

2.4

Information on product liability

In the following cases, the designated protection of the device may be affected. Liability for any resulting damage or personal injury is then transferred to the owner:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables that are not recommended by Eppendorf.

• The device is maintained or repaired by persons not authorized by Eppendorf AG.

• The user makes unauthorized changes to the device.

10

Safety


English (EN)

2.5

Warnings for intended use

Before using the device, read the operating manual and observe the following general safety instructions.

2.5.1

Personal injury and damage to device

DANGER! Unconsciousness and death by elevated levels of CO

2

Elevated levels of CO

2

may be found in the operating area of the CO

2

incubator.

 Wear personal protective equipment.

 If the laboratory is not properly ventilated, use a CO

2

/O

2

alarm system.

 Check the tube connection system with a leakage test.

 Read the Information on performing a risk assessment for the operation of incubators with

CO

2

and N

2

from Eppendorf AG.

WARNING! Burns during the high-temperature disinfection cycle

 Do not open the equipment door during the high-temperature disinfection cycle.

 Do not touch the equipment during the high-temperature disinfection cycle.

WARNING! Risk of personal injury

 Do not open the device!

 Do not operate a broken device! ( e.g.

if the exterior is damaged)

 Do not modify the device!

WARNING! Risk of personal injury and equipment damage

The incubator or incubators may topple if they are not fixed with a safety latch.

 Each incubator or stack of two incubators on a stacking stand must be fixed to the wall with a safety latch.

CAUTION! Risk of injury due to lifting and carrying of heavy loads

The device is heavy. Lifting and carrying the device can lead to back injuries.

 Transport and lift the device with an adequate number of helpers only.

 Use a transport aid for transporting the device.

CAUTION! Risk of injury when lifting and carrying heavy loads

 Use a lifting aid for the installation of the device.

Safety


English (EN)

11

CAUTION! Risk of personal injury

 Safety standards of lab must be considered.

 Use your personal protective equipment.

CAUTION! Risk of personal injury through infection or contamination

 Use your personal protective equipment.

 Disinfect the device with an alcoholic solution.

 Perform the high temperature disinfection, if the device comes with this option.


 Before closing the glass door be sure that the shelves well installed inside the chamber.

Slamming the glass door against the shelf could cause broken glass and potential injury.

 Never lean on or place objects on the open door.

 Do not put your fingers between door and housing while closing the door.


 More than one person is required to safely lift the incubator.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of accessories and spare parts other than those recommended, or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Damage of the incubator

Lifting the incubator by the door causes permanent damage to the incubator.

 Lift the incubator by its housing.

 Never lift the incubator by its door.

NOTICE! Risk of material damage

 Never put any liquid material on the top of the incubator. Spilled liquid could cause short circuit; this would cause permanent damage to the incubator.


 To avoid possible damage to the CO

2 sensor, never leave water in the water tray while the incubator id switched off, or when a high-temperature disinfection cycle (otion) is initiated.

 Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor removal.

12

Safety


English (EN)



2

sensor, never forget to remove the cover of the sensor during the high-temperature disinfection


 CO

2

gas pressure at the in-line pressure regulator must not exceed 0.05 MPa (0.5 bar,

7.2 psi).

 For O

2

option: N

2

gas pressure must not exceed 0.1 MPa (1 bar, 14.5 psi) at the in-line pressure regulator.

 For O

2

option: Remove the O

2

sensor when using high-temperature disinfection.


 The instrument or equipment, and its external connections, to be used inside the chamber should be specified as suitable for use in a humid environment, and at 37 °C. If in doubt, consult with the manufacturer of the equipment.

 Always ensure the connections are properly and securely made.

 Instruments bring heat into the chamber. Do not bring too much heat into the chamber.

Too much heat will make trouble in the temperature control; this could cause the loss of sample.


 Do not modify the device; this could cause the loss of sample.

2.6

Application limits

2.6.1

Description of ATEX Guideline (94/9EC)

DANGER! Explosion hazard

 Do not operate the device in areas where work is completed with explosive substances.

 Do not use this device to process any explosive or highly reactive substances.

 Do not use this device to process any substances which could create an explosive atmosphere.

DANGER! Explosion hazard

 Use gases in this equipment only within the range between their lower explosion limit

(LEL) and their upper explosion limit (UEL).

 If your process requires or produces gases, be sure to verify their LEL and UEL concentration range (available online or ask your gas supplier).

Safety


English (EN)

13

CAUTION! Lack of safety due to incorrect use of materials

 Do not use this device to process any flammable materials, or use materials where the transfer of mechanical energy to glass apparatus could lead to breakage.

Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially explosive atmospheres.

The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab.

The use of substances which may contribute to a potentially explosive atmosphere is not permitted.

The final decision regarding the risks associated with using these types of substances is the user's responsibility.

2.7

Symbol

Warning signs on the device

Meaning

Hazard point

Location

Hot surface

Operating manual must be read

14

Safety


English (EN)

Product description


English (EN)

15

3

3.1

Abb. 3-1: Front and back view

Product description

Product overview

1 2 3 4 5 6 7

15

Fig. 3-1: Front and back view

1 Operating controls

2 BMS relay contact alarm socket

3 RS232 socket

4 CO

2

sample port

5 Autozero filter

6 Main/power cord receptacle

7 On/Off switch

8 In-line pressure regulator CO

2

14 13 12 11 10

9 In-line pressure regulator N

2

(optional)

10 Gas box

11 O

2

sensor port (optional)

12 25 mm access port

13 Humidity control

14 Handle for outer door (with integrated heated viewing window)

15 Handle for opening of viewing window

8

9

16

Product description


English (EN)

3.1.1

Inside the chamber

1 2 3 4

1 Sample port

2 N

2

inlet (option)

3 CO

2

inlet

4 Door switch

5 Access port

10

9

8

7

6 Water level sensor (option)

7 O

2

sensor (option)

8 CO

2

sensor

9 Temperature probe

10 Humidity sensor (option)

5

6

Product description


English (EN)

17

3.2

Control panel

The control panel consists of an LCD display, 5 function keys and 4 direction keys.

Abb. 3-2: Control panel

1

2

Fig. 3-2: Control panel

1 Directional keys

Move cursor around the screen

Adjust values

2 Function keys

Purpose of each key is identified at the bottom of the display (above the corresponding key)

Function may change from screen to screen

The HELP file contains most of the information in this operating manual, together with more

detailed troubleshooting information (see HELP MENU screen on p. 41) .

18

Product description


English (EN)

3.3

Delivery package

1–2+2

1–2

1

1

1–2

1

1

4

1

4

2

2

1

1

Tab. 3-1: Accessories

Quantity Item

1

1

3

2

Device

Operating manual

Non-tip perforated shelves

Shelf racks

16+4

Water tray

Auto-zero HEPA filter

Adjustable feet

Plug for access port

Plugs for threads for stacking stand assembly

Silicone rubber suction feet

Notes

Provided

Provided

Installed

Installed

Installed

Installed

Installed

Installed

Installed

White porous CO

2

sensor cover

Black sensor cover

Plug for O

2

sensor

Power cord

10 mm (0.4 in) outer diameter tubing with

6.5 mm (0.2 in) inner diameter with large white filter

Anti-slip pads for adjustable feet

BMS standard 6-pin DIN plug

Safety fastening kit

Jaw wrench for adjusting the feet

Installed

Additional 4 spares packed in accessories bag

Installed (depending on options)

Additional 2 spares packed in accessories bag



Packed in box inside outer carton

Packed in accessories bag (depending on options)

Packed in accessories bag




3.3.1

Inspection of boxes

Inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local Eppendorf sales order department immediately.

3.3.2

Packing list verification

Unpack your order, saving the packing materials for possible future use. Save the operating manual for instruction and reference. Verify against your Eppendorf packing list that you have received the correct materials, and that nothing is missing. If any part of your order was damaged during shipping, is missing, or fails to operate, contact customer service.

Product description


English (EN)

19

3.4

Features

The Galaxy 48 R CO

2

incubator is microprocessor-controlled and designed to ensure accurate and reliable operation.

3.4.1

Operating controls

The incubator incorporates a sophisticated control system that allows for easy programming, control and monitoring of the chamber conditions.

3.4.2

Direct heating system

The direct heating system, utilizing a thermal heating element, completely surrounds the incubator, providing an even temperature within the chamber. The independently and directly heated outer door is designed to ensure an even distribution of heat. This system ensures a rapid, controlled return to optimum chamber conditions after an outer door opening while also preventing any overshoot. The incubator’s direct heat system provides for optimal use of laboratory space by allowing the most efficient internal volume for the footprint of the instrument.

3.4.3

Infrared sensor

A solid-state infrared sensor controls the level of CO

2

. It provides excellent reliability while remaining unaffected by humidity. The CO

2

system has a semi automatic zero system (Auto-zero) to re-reference the sensor baseline to atmospheric CO

2

levels at regular intervals. This provides accurate CO

2

control.

3.4.4

Water tray

A water tray at the bottom of the incubator creates a high, uniform relative humidity (RH) while preventing condensation in other parts of the chamber. Perforated shelves are provided as standard to facilitate recovery of RH conditions in the chamber.

3.4.5

Seamless chamber

The 48 Liter chamber is seamless, and provides a sanitary and easy-to-clean environment. All internal components are manufactured from polished stainless steel. The non-tip shelves, shelf racks and water tray are easily removeable without tools for thorough cleaning and are able to be sterilized. Air circulation is achieved without the use of a fan, which eliminates ductwork (a potential source of contamination), simplifies cleaning, eliminates vibration, and facilitates use of microplates and low-volume culture.

3.4.6

Standard features

The Galaxy 48 R contains many standard features usually seen as options. It has a sealed glass door with a covert window to allow viewing of the cultures without compromising the internal atmosphere. Also available is a 2-split-door option (to coordinate with shelves), which is ideal for critical hypoxic studies. In addition, there is a 25 mm (1 in) access port to allow for seamless integration of independent probes or other equipment through the chamber. A building management system (BMS) relay is standard on every incubator and allows alarms to be transmitted to a central monitoring system. All units are also equipped

20

Product description


English (EN) with an RS-232 port that allows the incubator to be connected to a PC for the recording of operating parameters.

3.4.7

Multiple options

The incubator features multiple options that can be installed to simplify maintenance and provide superior control over experimental conditions.

For example, high-temperature disinfection quickly and conveniently disinfects the incubator’s chamber at

120 °C, without the need to remove interior components or the CO

2

sensor (except the optional O

2

-sensor).

A warning system warns the user before the water tray runs out of water, preventing dehydration of samples. The water tray must be emptied and the device must be clean and dry prior to running high-temperature disinfection.

Oxygen control provides for conditions that require below-ambient oxygen levels. For a complete list of the

available options, (see Available options for device on p. 73) .

3.4.8

Two-level alarm system

The incubator incorporates a two-level alarm system. The system alarms occur only, if a problem develops with system components that require user intervention to rectify. The incubator also incorporates an over-temperature safety system that operates independently from the main control system.

3.5

Stacking devices

The incubator is not designed to be directly stackable. A second incubator may be safely stacked on top of another identical incubator by using the custom-designed stacking stand available as an accessory. It is not possible to put any other type of incubator or heavy apparatus on top, as the top cover and stacking stand were not designed to support any other device.

3.6

Connectivity

Galaxy 48 R can be integrated into the Eppendorf connectivity solution VisioNize, which allows monitoring and management of connected devices. VisioNize connectivity enables remote monitoring of device performance and notification of important events by email. More detailed information regarding VisioNize can be found on our webpage, www.eppendorf.com. Accessories required for connecting to VisioNize system can be found in chapter Ordering information > Connectivity .

Installation


English (EN)

21

4

4.1

Installation

Selecting the location


 The device has to be installed on an even, stable ground to avoid falling down.

CAUTION! Lack of safety due to humid storage and transport conditions

 Device needs to be evaluated for condensation when received and unpacked for installation.

 If condensation due to storage and transport in humid conditions has occurred, allow a dry out for 4 hours in an environment of 18–25 °C and 30–80 % relative humidity. Only with a drying-out process, the device is able to meet safety requirements.


Due to an unlevelled device not all cells are covered with the medium; this could cause the loss of sample.

 Level the device by adjusting its feet.

Select a level surface capable of withstanding the operating weight of the incubator. Actual operating weight will be dependent on both the options installed, and the material stored in the incubator.

Position incubator to allow clearance for opening door and access to the power cord/power switch and to the CO

2

sample port located on the rear side of the incubator.

The correct location features

• well ventilation

• protection from direct sunlight

• protection from UV radiation

• a clear distance to cold or heat sources, e.g. heaters or drier compartments

• a minimum ventilation clearance of 10 mm (0.5 in) all around

• enough space on the left side to use the mains/power switch and to unplug the mains/power cord

The electrical connections feature

• a matching of mains/power connection with the specifications on the name plate

• access to the mains/power switch and cutting unit of the power system circuit is accessable during operation

22

Installation


English (EN)

4.2

Utilities requirements

The following utility requirements are needed to operate the incubator:

Tab. 4-1: Utility requirements

Utility

Electricity

Requirement

110 V – 127 V, 50/60 Hz earth/grounded mains/electrical supply with minimum capacity of 10 amps.

Mechanical

CO

2

gas

For O

2

option: N

2

gas

220 V – 240 V, 50/60 Hz earth/grounded mains/electrical supply with minimum capacity of 8 amps.

Safety Fastening Kit for fixing one incubator or two stacked incubators to the wall.

Cylinder with 100 % CO

2

vapor withdrawal, together with a two-stage regulator for pressure control to 18.85 psi (1.3 bar).

Cylinder with 100 % N

2

vapor withdrawal, together with a two-stage regulator for pressure control to 21.7 psi (1.5 bar).

 CO

2

gas pressure at the inline pressure regulator must not exceed 0.05 MPa (0.5 bar, 7.2 psi).

 Only in case of using the low O

2

option it is necessary to use a higher CO

2

pressure at the inline pressure regulator: At 0.1 % O

2

 N

2

setting use 0.1 MPa (1.0 bar, 14.5 psi) CO

2

.

gas pressure at the inline pressure regulator must not exceed 0.1 MPa (1 bar, 14.5 psi).

4.3

Initial setup

4.3.1

Installing the feet


 More than one person is required to safely lift the incubator.

To ensure adequate airflow for correct operation of the humidity control system, adjustable feet must be installed:

1. Install the locking nuts onto each of the 4 feet provided.

2. Tilt the incubator back and install the front feet in to the required depth.

3. Tilt the incubator forward to install the rear pair of feet.

4. Place an anti-slip pad (provided) on each foot.

Keep anti-slip pads installed at all times.

Installation


English (EN)

23

4.3.2

Install the power cord

1. Insert the power cord into its receptacle on the back on the incubator.

2. Press the cord firmly into its socket.

4.3.3

Making connections

DANGER! Unconsciousness and death by elevated levels of CO

2

Elevated levels of CO

2

may be found in the operating area of the CO

2

incubator.

 Wear personal protective equipment.

 If the laboratory is not properly ventilated, use a CO

2

/O

2

alarm system.

 Check the tube connection system with a leakage test.

 Read the Information on performing a risk assessment for the operation of incubators with

CO

2

and N

2

from Eppendorf AG.

WARNING!

 Observe the national standards and regulations for the use/handling of gas.

 Installation and connection of gas tubing has to be done by educated personnel.


 Remove the protective covers when putting the device into routine work. If the covers remain and cover the sensor, e.g. CO

2

will continuously flow into the incubator and possibly leak out of the device.

WARNING! Danger due to incorrect power supply

 Connect the device to voltage sources that meet the requirements on the name plate.

 Use sockets with a protective earth (PE) conductor and suitable power cable.


The gas tubing and in-line gas filter will burst or crack due to high pressure.

 Incoming CO

2

gas pressure must not exceed 0.2 MPa (2 bar, 29 psi).

1. Remove the protective cover of CO

2

, O

2

, or RH sensor (option dependent). Save the cover for reuse when the incubator is to be cleaned.

2. Attach the supplied 6.5 mm bore tubing with large white filter to the two-stage pressure regulator outlet. Secure the connected end with a clip (not supplied).

3. Attach the supplied 6.5 mm bore tubing with large white filter to the gas inline pressure regulator. Push the tubing into the rotatable tube connector of the inline regulator until it stops.

24

Installation


English (EN)

Abb. 4-1: Pressure regulator outlet

4

3

1

2

Fig. 4-1: Pressure regulator outlet

1 Inline pressure regulator CO

2 Inline pressure regulator N

2

2

3 Tube connector N

2

4 Tube connector CO

2

The in-line pressure regulator controls Secondary Gas Pressure. A default pressure setting for

CO

2

of 0.05 MPa (0.5 bar/7.2 psi) is recommended. O

2

option: A default pressure setting for

N

2

of 0.1 MPa (1.0 bar/14.5 psi) is recommended. Adjust the regulator knob to the required outlet pressure and purge tubing, making the final adjustments when the gas is flowing. A large size cylinder of CO

2

- Vapor Withdrawal is required to supply the incubator. This cylinder controls Primary Gas Pressure. Fitting a two-stage CO

2

pressure regulator is recommended.

Ensure the protective covers are removed from all sensors. Carefully remove the black CO

2 sensor cover. Do not remove the white porous sensor cover. The white cover must remain in place.

4. Check if the tubing is fixed by carefully pulling on it

For disconnecting the tubing, press down the small ring of the tube connector and pull out the tubing.

5. Check the pressure settings of the CO

2

in-line pressure regulator and (if connected) the N

2

in-line pressure regulator. The pressure unit is shown in MPa at the in-line pressure regulator.

6. Confirm the voltage requirements with the label information.

7. Connect incubator to mains/voltage supply.

Installation


English (EN)

25

8. Secure the incubator to the wall with a safety latch. Follow the instruction for the Safety Fastening Kit.

Use the mounting point close to the position of the Auto-zero filter.

1

1 Safety latch

4.3.4

Replaceing the shelf racks and shelves, and level the incubator

The shelf racks and shelves are pre-installed. Replace the shelves or shelf racks, if required:

Abb. 4-2: Shelf rack

R

1. Each shelf rack has silicone suction cups that hold the rack in place. Install the silicone suction cups onto the rack supports (8 per rack).

2. There are left-hand and right-hand racks. The suction cup marked R in the figure goes to the rear of the chamber.

The suction cups will adhere to the chamber walls even if they are dry. If it is necessary, dampen the cups with distilled water to increase adhesion.

Fig. 4-2: Shelf rack

26

Installation


English (EN)

Abb. 4-3: Suction cup 3. Ensure the shelf racks are installed squarely in the chamber so the shelves are on a level plane.

Fig. 4-3: Suction cup

Abb. 4-4: Shelf rack 4. Install the 3 shelves, making sure that each shelf´s anti-tip tab is properly inserted onto each of the shelf rack guides.

5. Level the incubator by adjusting the feet: Place a small level on the second shelf of the incubator and adjust the leveling feet until the incubator is level and stable. Lock the leveling legs in place by tightening the locking nuts on each leg.

1

Fig. 4-4: Shelf rack

4.3.5

Replacing the water tray

The water tray is pre-installed. If replacement is required, install the water tray in its location beneath the lowest shelf rack position:

Abb. 4-5: Water tray 1. Insert the tray diagonally as shown, then turn the tray 45° to set it in position.

Fig. 4-5: Water tray

Abb. 4-6: Water tray

1

Installation


English (EN)

27

2. Position the tray on the chamber floor, with its front and rear edges resting just above the shelf rack supports.

Fig. 4-6: Water tray

3. Check that the water tray rests fully on the chamber floor. A gap between tray and chamber floor could result in condensation under the tray.

4.3.6

Replacing the Auto-zero filter

The Auto-zero filter is pre-installed. If replacement is required:

1. Press the Auto-zero filter gently into the filter socket at the top of the rear panel.

28

Installation


English (EN)

Operation


English (EN)

29

5

5.1

Operation

Preparing for operation


 Do not forget to remove the sensor protective covers when putting the device into routine work. If the covers remain on the sensors, e.g. CO

2

will continuously flow into the incubator and possibly leak out of the device.


The gas tubing and in-line gas filter will burst or crack due to high pressure.

 Incoming CO

2

gas pressure must not exceed 0.2 MPa (2 bar, 29 psi).

1. Remove the black protective cover from the CO

2

sensor, taking care not to remove the white porous cover.

Save the black cover for reuse when the incubator is to be cleaned.

2. Connect the incubator to a earthed/grounded power supply.

3. Switch the incubator on.

The display will illuminate immediately.

4. Turn on the CO

2

gas supply. The pressure regulator of the gas supply is to be set to 0.05 MPa (0.5 bar/

7.2 psi) unless operating with N

2

or O

2

.

5. The chamber setpoints are pre-programmed at 37 °C and 5 % CO

2

. Leave the incubator on until the programmed chamber temperature and CO

2

concentration have been reached.

• The CO

2

valve is disabled until the incubator reaches the temperature setpoint. After the temperature setpoint is reached, the CO

2

valve is activated.

• If power is interrupted to the incubator long enough for the temperature to drop below setpoint, the CO

2

valve will be deactivated until temperature setpoint is achieved again.

This procedure serves to avoid spurious CO

2

readings.

6. Leave the incubator running for at least two hours (preferably overnight) to allow conditions to stabilize.

CO

2

sensors are calibrated at the factory to control accurately at 5 % CO

2

and 37 °C. Changing the temperature or the CO

2

value setpoints leads to deviations in accuracy.

 Close the outer door properly to avoid condensation or contamination.

30

Operation


English (EN)

5.2

Using the water tray



2

sensor, never leave water in the water tray while the incubator is switched off, or when a high temperature disinfection cycle is initiated

(optional feature).


• The water tray has to be left in place at all times.

• Use distilled water only in the water tray. The usage of any other types of water including deionized water and chlorinated additives will cause corrosion inside the incubator.

• Do not move the incubator, when the water tray is filled. Spilled water could cause corrosion in the chamber.

If humidification is required:

1. Fill the water tray with 0.5 L of warm distilled water (around 37 °C).

2. To reduce the risk of contamination, clean the tray every 10–14 days with a solution of 70 % isopropyl alcohol and 30 % distilled water.

Refill the tray with 0.5 L of warm distilled water when clean.

The humidity level within the chamber is not adjustable. The chamber will reach between 85 and 95 % relative humidity at 37 °C (depending on ambient humidity) using the water tray.

5.3

Programming

5.3.1

Programming CO

2

, O

2

and temperature

Perform the following steps to set the desired operating temperature, O

2

and CO

2

level.

1. Press the PROG function key.

2. In the PROG screen that appears, press the desired function key, TEMP , O

2

or CO

2

, then use the and

direction keys to adjust the value.

If the incubator is supplied with the option of oxygen control, the setpoint for the oxygen level can be selected and changed like the temperature and CO

2

setpoints.

3. When the desired setpoint is displayed, press the ENTER function key.

4. After making adjustments (if any were made), allow the incubator to stabilize at the setpoints before continuing.

If the chamber temperature goes above the temperature setpoint by 1 °C, the over-temperature system will activate.

Operation


English (EN)

31

Program the required oxygen level in the PROG screen, following the onscreen instructions.

If you are running an O

2

level programmed between 0.1 - 0.9 %, you should know that the control system is set to operate in the following way to minimize N

2

consumption after the outer door has been opened:

• The N

2

valve is switched on continuously until the O

2

level is within 0.1 % of setpoint.

• The CO

2

valve is then switched on to allow the CO

2

level to reach setpoint. If the O

2

level is above setpoint 15 minutes after the N

2

valve has been switched off, it is switched back on for 40 seconds and the CO

2

valve is switched on for 20 seconds. The CO

2

valve will then pulse until setpoint is reached.

• The process described above will repeat itself until the O

2

setpoint is reached.

• The same process will also repeat if the O

2

level rises above setpoint, and if the O

2

level should rise toward 0.2 % above setpoint, the N

2

valve will open again continuously until the O

2

level returns to setpoint.

• The CO

2

autozero, which would normally take place after a CO

2

alarm, will be cancelled to avoid the introduction of additional O

2

into the chamber. For the same reason, we recommend canceling the programmed CO

2

autozero.

5.3.2

User access code

Programmable user access code allows you to restrict access to the PROG , USER , and ALARM screens

(where settings can be changed) to authorized persons only.

To set the user access code (if required):

1. Press the PROG function key to enter the PROG screen.

The user access code will be displayed as a series of 4 asterisks.

2. Use the left and right direction keys to move to each code position, and the up and down direction keys to select a number from 0 – 9.

3. Once the number is selected, press the ENTER function key to save the code.

4. After returning to the main screen, programming access will require the code to make any further programming changes.

Take care to note your password somewhere. If a password is forgotten, you must contact a customer service representative to recover or delete the forgotten password.

5.3.3

Removing user access code

1. In the PROG screen, enter the current access code.

2. Now program 0000 as the new access code.

3. Press the ENTER function key to save the change.

The code is now cancelled and programming is no longer restricted.

If the access code has been misplaced, you will be unable to make changes to your incubator’s settings. Contact customer service or your service representative for instructions on how to regain access to your incubator.

32

Operation


English (EN)

5.4

Referencing the CO

2

sensor with autozero

Prior to using the incubator, you should manually perform a CO

2

Auto-Zero (see PROGRAMMABLE CO

2

AUTOZERO on p. 33) :

1. Perform a CO

2

autozero by pressing the USER

function key (see USER SETTINGS on p. 32) , selecting

PROGRAMMABLE CO

2

AUTOZERO , and pressing the START key.

2. The incubator will display a countdown as the autozero is running.

3. When the countdown is complete, the incubator is ready to use.

5.5

USER SETTINGS

In the USER SETTINGS screen, you can adjust the features called out on the screen.

Abb. 5-1: USER SETTINGS screen

1

2

Fig. 5-1: USER SETTINGS screen

1 Use the and direction keys to move the cursor

2 Use the ENTER function key to select an option

This section explains each of the USER screen features. There are other USER options that may be

displayed on screen if they are installed on your incubator. For a list of available options (see Available options for device on p. 73) .

5.5.1

SET DATE AND TIME

The date and time is factory set and will only require adjustment if you are in a different time zone, or when you change your clocks to Daylight Saving Time and back again to standard time. You may also select the style of display for the date.

Operation


English (EN)

33

5.5.2

AUDIBLE ALARM VOLUME ADJUST

The audible alarm volume can be adjusted to your own preferences.

5.5.3

PROGRAMMABLE CO

2

AUTOZERO

When you select this feature, the PROGRAM CO

2

AUTOZERO

screen (see Fig. 5-2 on p. 33) allows you to

program the autozero frequency and time, or to run the autozero function manually.

Autozero the CO

2

system:

• Prior to using the incubator for the first time.

• Once a month when your incubator is operating, to ensure that the CO

2

level is as accurate as possible.

• After the incubator has been in storage (or transit) for a while.

The autozero system automatically re-references the CO

2

sensor to atmospheric CO

2

in the following way:

1. A pump activates for 2 minutes, pumping atmosphere at 0.3 Liters/minute into the sensor’s measuring chamber. This displaces the chamber atmosphere completely from the sensor.

Abb. 5-2: PROGRAM CO2 AUTOZERO screen

Fig. 5-2: PROGRAM CO

2

AUTOZERO screen

This procedure does not affect the chamber environment and will not affect your cell culture as it is being performed.

2. After the pump shuts off, the control system adjusts the autozero factor to reference the sensor to

0.05 % CO

2

, which is the approximate atmospheric level.

3. The pump switches off and the chamber atmosphere diffuses back into the sensor’s measuring chamber. This takes 3 minutes, after which the normal CO

2

control system takes over.

4. The result of the autozero (listed as A/Z on some screens) is sent to the DATALOGGER ALARM EVENTS screen so that a record of the results will be kept.

The frequency of autozeroing can be set in steps between once a day and once every 28 days. The default

setting is once every 28 days. If not required, it can be disabled (see DISABLE on p. 34) .

34

Operation


English (EN)

The default time setting is 7:00 am. This can be altered to suit your requirements. Only change the time setting shortly before you start to use the incubator.

The autozero will only occur if the temperature is at setpoint. If the temperature is not at setpoint, the system will postpone autozero until the setpoint is achieved.

If the autozero function is to be run manually, simply press the START function key, within the PROGRAM

CO

2

AUTOZERO window.

5.5.4

DATALOGGER

For detailed information (see DATALOGGER on p. 35) .

5.5.5

POWER FREQUENCY

You can adjust the power frequency to either 50 or 60 Hz to match the local mains/electrical supply. Use the or direction key until the correct frequency is displayed, then press the ENTER function key.

5.5.6

DISABLE

This feature allows you to inform the control system to ignore certain sensors if their function is not required. The standard item on this menu is the CO

2

PRESSURE SWITCH (for autozeroing). Additional

disable options appear on this screen according to the options installed on your incubator, (see Available options for device on p. 73) .

To disable a feature, scroll to OFF using the and direction keys, then press the ENTER function key.

5.5.7

DISINFECTION

(optional)


 Make sure that the water tray is empty and dry, and that the O

2

sensor is removed before running the high-temperature disinfection.

 For O

2

option: Remove the O

2

sensor.

If the incubator is supplied with the high-temperature disinfection option, the menu item DISINFECTION will be displayed. This feature activates the disinfection cycle of the incubator.

The disinfection cycle heats the chamber to 120 °C, holds that temperature for 4 hours, and then cools the chamber to the selected temperature setpoint. All of the interior components (with the exception of the O

2 sensors, if present) can be left in place during the cycle to ensure that everything within the chamber is

disinfected prior to resumption of activity. For a full explanation of this feature, (see High-temperature disinfection on p. 41) .

5.6

DATALOGGER

The DATALOGGER screen displays the following information:

Abb. 5-3: DATALOGGER screen

Operation


English (EN)

35

Fig. 5-3: DATALOGGER screen

5.6.1

ALARM EVENTS

The following alarm events are recorded in the order in which they occurred, with the most recent event displayed at the top:

• Power ON/OFF

• Chamber Temperature High/Low (programmed value)

• CO

2

Level High/Low (programmed value)

• CO

2

Supply Failure

• All System Alarms

• CO

2

Autozero (A/Z) Adjustments

• Oxygen and Relative Humidity (R/H) Alarms (where these options are installed)

The capacity is 99 events, after which the earliest event is overwritten and a later event is added.

The date and the time are also recorded for each event (see Fig. 5-4 on p. 36):

36

Operation


English (EN)

Abb. 5-4: ALARM EVENTS screen

Fig. 5-4: ALARM EVENTS screen

5.6.2

TEMPERATURE GRAPH + DOOR OPEN BAR CHART

When you select this from the DATALOGGER screen, the Door Open bar chart is shown at the top of the

screen to associate it with a temperature disturbance (see Fig. 5-5 on p. 37). A temperature reading is

recorded every 18 seconds while the temperature is outside the specification of ±0.1 °C and each reading is shown as a single pixel.

When the temperature has settled within specification, the recording is compressed to one pixel representing (10) 18-second readings (as long as the temperature remains in specification). This allows up to 10 hours of readings to be displayed on one screen. When the temperature moves outside specification, for instance if the outer door is opened, the graph reverts to individual 18-second readings until temperature is within specification again.

When the data is compressed or decompressed, a light dotted line is displayed vertically on the screen to

signify that the time axis is changing from 18-second to 10 x 18-second increments or vice versa (see

Fig. 5-6 on p. 37).

A heavy dotted line (not shown) is displayed when the incubator is switched on.

Abb. 5-5: TEMPERATURE GRAPH + DOOR OPEN BAR CHART screen

Fig. 5-5: TEMPERATURE GRAPH + DOOR OPEN BAR CHART screen

Abb. 5-6: Dotted vertical line showing compressed/decompressed data.

Operation


English (EN)

37

Fig. 5-6: Dotted vertical line showing compressed/decompressed data.

Compressing data allows memory space to be maximized. Once the memory space has been filled, the earliest events are overwritten as they are replaced by the latest recording. Graphical recording can be extended, however, to a number of years if your incubator is equipped with an RS-232 port, by connecting the port to a PC loaded with BioCommand ®

SFI Software (see Accessories on p. 73)

.

5.6.3

CO

2

GRAPH + DOOR OPEN BAR CHART

These graphs record in a way similar to the Chamber Temperature graphs. The specification for CO

2

is

±0.1 %.

Both CO

2

and temperature graphs share the same time axis. If the time axis changes to accommodate data in one graph, it will also change in the other graph.

38

Operation


English (EN)

5.6.4

DIAGNOSTIC CHAMBER ELEMENT GRAPH

This graph records chamber element temperature over time to assist troubleshooting.

5.6.5

DIAGNOSTIC DOOR GRAPH

This graph records the door’s inner surface temperature over time to assist troubleshooting.

5.6.6

DIAGNOSTIC DOOR ELEMENT GRAPH

This graph records door element temperature over time to assist troubleshooting.

5.6.7

RESTART GRAPHIC RECORD

This feature removes the current graph and begins a new one.

The data cannot be recovered once it is deleted.

5.7

CHAMBER ALARMS

To enter the CHAMBER ALARMS menu screen, press the ALARM function key on the main display. The

CHAMBER ALARMS

programming screen (see Fig. 5-7 on p. 38) allows the various alarm options to be

selected and modified. Press the or direction key to move around the options and the or direction key to adjust values. The temperature and CO

2

high and low alarm setpoints automatically adjust to within ± 0.5 of the temperature and CO

2

setpoints. The alarm setpoints can also be manually adjusted.

Abb. 5-7: CHAMBER ALARMS screen

Fig. 5-7: CHAMBER ALARMS screen

Operation


English (EN)

39

5.7.1

Arm chamber alarms after selectable delay

1. Choose the option ARM ALARMS WHEN AT SETPOINT .

2. Select NO for both TEMP and CO

2

(see Fig. 5-7 on p. 38).

3. Choose the option DELAY IN ARMING AFTER DOOR OPEN and select the desired delay (15 minutes in

the sample screen (Fig. 5-7 on p. 38)) to allow for temperature and CO

2

recovery after the outer door has been opened.

5.7.2

Arm chamber alarms after setpoints are achieved

1. Choose the option ARM ALARMS WHEN AT SETPOINT .

2. Select YES for both TEMP and CO

2

.

3. When YES is selected for this function, the DELAY IN ARMING AFTER DOOR OPEN is ignored.

5.7.3

Door open alarm

The DOOR OPEN ALARM can be adjusted and chosen from 7 preset durations (45 seconds in this example),

(Fig. 5-7 on p. 38) to warn of an improperly closed outer door.

5.7.4

Audible and visual alarms

The audible and visual alarms can be adjusted from off to on (which means the alarm will be on continuously until it is acknowledged) in 7 preset time increments.

In the off position, any chamber alarms that occur will be displayed on the screen without flashing and with

the audible alarm inhibited (see Fig. 5-7 on p. 38).

5.7.5

Chamber alarm system function

When the incubator is switched on, or after the temperature and CO

2

levels have been re-programmed, the alarm system is inactive until the setpoint values are achieved (within ±0.1), after which the alarm system is armed. CO

2

and temperature alarms are individually armed.

If temperature and/or CO

2

levels deviate more than the programmed setpoints, the display flashes, the

audible alarm sounds and a message appears on the screen (see Fig. 5-8 on p. 40). Acknowledge the alarm

by pressing any key.

40

Operation


English (EN)

Abb. 5-8: CHAMBER ALARM message

Fig. 5-8: CHAMBER ALARM message

After setpoints have been achieved for the first time, when the outer door is opened, the alarm system is disabled. Once the outer door is closed, a programmable alarm delay starts (if selected):

• If chamber conditions recover within the programmed alarm delay time, the alarm system is immediately re-armed. After the delay, the alarm system is armed and if the temperature and CO

2

are outside the alarm high and low settings, the alarm will be activated.

• If an alarm occurs and the chamber subsequently recovers, the alarm stops and the system is re-armed.

Details of the alarm event are stored in the datalogger.

If the CO

2

valve is opened and no pressure is detected, an alarm occurs and a warning message appears on the screen, alerting you to CHECK CO

2

SUPPLY

(see Fig. 5-9 on p. 40).

Abb. 5-9: CHAMBER ALARM to check CO2 supply

Fig. 5-9: CHAMBER ALARM to check CO

2

supply

Instructions to remedy the alarm are provided in the ALARM screen.

5.8

DIAGNOSTICS menu screen

The DIAGNOSTICS screen contains technical information regarding the status of many of the system components found on the incubator. This screen is mainly for technical service use, and can be used to

Operation


English (EN)

41 troubleshoot the incubator systems before service is scheduled. This information allows technical support to optimize the service support required, and to shorten service time.

Abb. 5-10: DIAGNOSTICS screen

Fig. 5-10: DIAGNOSTICS screen

5.9

HELP MENU screen

The HELP MENU screen provides user-selectable categories of abbreviated information found in the user manual. All the major systems are covered in the help menu, including help on installing the incubator. If the user manual is misplaced, information about the CO

2

incubator and its functions can always be found on-screen.

Abb. 5-11: HELP MENU Screen

Fig. 5-11: HELP MENU Screen

5.10

High-temperature disinfection

The high-temperature disinfection option is designed to heat the internal chamber to 120 °C, maintain that temperature for 4 hours, and then allow the chamber to cool down to 37 °C or to the programmed

42

Operation


English (EN) temperature (if different from 37 °C) when normal control takes over. The cycle is designed to disinfect all

internal surfaces and components, with the exception of the oxygen control sensor where supplied (see

High-temperature disinfection option with oxygen control on p. 44)

.

5.10.1

Using the high-temperature disinfection

Prerequisites

• The incubator must be cleaned, disinfected, and dried thoroughly before starting the cycle, (see

Cleaning on p. 62)

.

• The black protective cover must be removed (the white porous cover can remain in place).

• The shelves, shelf racks, water tray and silicone rubber feet and sleeves should all be in place during the cycle.

• The water tray must be empty, clean and dry.

WARNING! Burns during the high-temperature disinfection cycle

 Do not open the equipment door during the high-temperature disinfection cycle.

 Do not touch the equipment during the high-temperature disinfection cycle.



2

sensor, never leave water in the water tray while the incubator is switched off, or when a high-temperature disinfection cycle (option) is initiated.


1. Press the USER menu button, select DISINFECTION and press START . The incubator will then prompt:

IS CHAMBER CLEAN & DRY?

Answer YES if it is clean and dry.

The cycle will start automatically, unless the incubator is fitted with oxygen control, in which case the incubator will also prompt: IS O

2

SENSOR REMOVED?

Ensure that the O

2

sensor has been removed and answer YES to begin the cycle.

2. To cancel the cycle, press CANCEL . The incubator will cool down to the programmed level where normal control takes over.

If an autozero is scheduled to begin prior to a disinfection cycle, the autozero will abort until the cycle is complete. A user initiated autozero will also abort but will not resume after completion of the disinfection cycle.

3. If the incubator outer door is opened during a disinfection cycle, the process will continue as normal, a failure message will occur due to low temperature.

Certain areas of the glass of the outer door and door seal surface temperatures will be ±5 °C of

120 °C.

4. After completion of the process, one of the following status messages will be displayed. If the cycle: was completed successfully, DISINFECTION COMPLETED OK is shown. was cancelled by the user, DISINFECTION WAS ABORTED is shown. failed for any reason, DISINFECTION FAILED [CODE: XX] is shown.

Operation


English (EN)

43

The following tables lists the disinfection failure codes and descriptions, (see Tab. on p. 43) and (see

Tab. on p. 43). If this happens, note the failure code and contact your service representative for advice.

0A

0B

0C

0D

06

07

08

09

0E

0F

Tab. 5-1: Disinfection failure codes and descriptions

Failure code

01

Failure code description

Z

02

03

04

05

W

W, Z

X

X, Z

W, X

W, X, Z

Y

Y, Z

W, Y

W, Y, Z

X, Y

X, Y, Z

W, X, Y

W, X, Y, Z

Tab. 5-2: Disinfection failure code explanations

Failure code description Explanation

W

X

Temperature drop during warm-up period: indicates the temperature fell more than 2 °C during the heating phase over a 60-second period.

Temperature drop during 4-hour period: indicates the temperature fell below 118.0 °C during the disinfection phase.

Y

Z

Temperature increase during cool-down phase: indicates the temperature rose by more than 2 °C during the cooling phase over a 60-second period.

Cancel key pressed.

• If the incubator power is cycled OFF then ON during a disinfection cycle due to a power outage, the incubator will power up as normal. This condition will be indicated by the absence of a completed disinfection status message ( DISINFECTION COMPLETED OK ).

• If the chamber temperature is above the setpoint or the element temperature is greater than a factory-preset control point, cool down will be entered until these conditions are satisfied.

• It is recommended that the autozero function be run following each disinfection cycle.

44

Operation


English (EN)

5.10.2

High-temperature disinfection option with oxygen control



2

sensor, never leave water in the water tray while the incubator is switched off, or when a high-temperature disinfection cycle (option) is initiated.


The oxygen sensor is an electrochemical device that will be destroyed by the high temperature used to disinfect the incubator if left in place. For this reason, the oxygen sensor must be removed from the incubator prior to a high temperature disinfection cycle. The sensor can be accessed from the rear panel of the incubator.

Detailed removal and installation instructions are provided (see Oxygen sensor removal on p. 44) .

5.11

Oxygen sensor removal

Prior to beginning a disinfection cycle, remove the oxygen sensor. For detailed instructions, (see Removing and replacing O

2

sensor on p. 51) .

1. Remove the black plastic plug that covers the oxygen sensor located on the back of the incubator.

2. Disconnect the sensor cable by unplugging the electrical connector (grasp the white connector body, not the wire leads).

3. Unscrew the oxygen sensor by turning it counter-clockwise. Never use excessive force or metal tools.

4. Store the sensor in a clean, safe place until the disinfection cycle is over.

5. The incubator is now ready to perform a disinfection cycle.

5.12

Alarm relay for Building Management System

The alarm relay for Building Management System (BMS) allows a signal from a central alarm system to be switched ON or OFF to indicate an alarm condition at the incubator.

The following alarm conditions will activate the system:

• Over-temperature

• Under-temperature

• System failure

• CO

2

high

• CO

2

low

As an integral option, the alarm can be programmed to indicate when the power fails (due to an electrical fault) or is switched off. If the power failure warning is active, the relay contacts will be reversed (pin 4, which is normally open, becomes normally closed and pin 6, which is normally closed, becomes normally

Operation


English (EN)

45 open). The alarm will also respond to other types of alarms, depending on the options installed on the incubator.

The system is connected at the rear of the incubator via a standard 6-pin DIN socket (see Fig. 5-12 on p. 45)

for location. The matching plug is provided, when the option is installed.

Only connect devices that meet the safety requirements defined in IEC 60950-1.

Abb. 5-12: BMS relay contact alarm socket

3

2

1

4

5

6

Fig. 5-12: BMS relay contact alarm socket

Pin

1

2

3

4

5

6

24 V DC unregulated

0 V

5 V DC

Normally closed

Common

Normally open

Designation

To power external equipment such as a remote buzzer or light

(100 mA maximum current available).*

Via 10 K pull-up resistor, for a logic signal to directly control an auxiliary control system.*

To access the relay contacts. Contact limits are 3 Amps @ 24 V DC and 3 Amps @ 34 V AC.

*Cable length should not exceed 3 m (9.8 ft) to comply with EMC requirements.

The default of Galaxy 48 R setting for the alarm system is ON. To deactivate the relay using the incubator keypad:

1. Tap USER .

2. Select BMS ALARM RELAY .

3. Select MAKE ALARM RELAY ACTIVE YES/NO .

4. Toggle to NO and then tap ENTER .

46

Operation


English (EN)

The default setting for the power failure warning is ON . To make the alarm system ignore any power outage:

1. Tap USER .

2. Select BMS ALARM RELAY .

3. Select MAKE ALARM RELAY ACTIVE AT POWER SWITCH OFF/FAILURE YES/NO .

4. Toggle to NO and then tap ENTER .

5.13

O

2

control

5.13.1

Setting up the N

2

tank

Before setting up O

2

control, ensure that you have the proper equipment for your nitrogen supply:

• 2 cylinders of nitrogen, regulation size W

• (1) two-stage pressure regulator

• 10 mm outer diameter tubing with 6.5 mm inner diameter

• Tubing clips (not supplied)

To set up the nitrogen tanks:

1. Inspect the tanks to ensure that there are no leaks or other damage.

2. Check that the two-stage pressure regulator valve and the inline pressure regulator valve are closed by trying to turn their knobs in a counter-clockwise direction.

The knobs should be at the stop point.

3. Attach supplied 6.5 mm bore tubing with large white filter to the gas inline pressure regulator’s inlet on

the left side of the control box (see Remove the protective cover of CO

2

, O

2

, or RH sensor (option dependent). Save the cover for reuse when the incubator is to be cleaned. on p. 23)

. Push the tubing into the rotatable tube connector of the inline regulator until it stops.

4. Check if the tubing is fixed by carefully pulling on it.

5. Set up oxygen control based on your option: 1–19 % or 0.1–19 %.

For disconnecting the tubing, press down the small ring of the tube connector and pull out the tubing.

5.13.2

Setting up oxygen control (0.1–19 %)

Oxygen control lets O

2

and CO

2

levels achieve at approximately the same time, via control of the N

2

valve's duty cycle (but within the range of 0.1–19 % O

2

).

1. Remove the black plastic protective cover (making sure that the hydrophobic filter cap is not removed with it) from the port inside the chamber. Retain the cover for use when you clean the chamber.

2. Open the nitrogen gas supply from the tank and set the N

2

tank’s outlet pressure gauge to 1.5 bar.

3. Set the N

2

inline pressure regulator to 0.1 MPa (1.0 bar, 14.5 psi). The gas flow rate is approximately 20 liters/minute.

Operation


English (EN)

47

• If the programmed O

2

level is close to the ambient oxygen, it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value.

• When working at 0.1 % O

2

, set the CO

2

pressure to 0.1 MPa (1.0 bar, 14.5 psi).

Be sure to humidify the incubator and leave it overnight to stabilize before proceeding further.

To automatically calibrate the sensor to atmospheric oxygen levels, select OXYGEN SENSOR – REF TO

ATMOSPHERE in the USER menu, and then follow the onscreen instructions.

The oxygen reading is automatically adjusted to 19.7 %, which is the true reading taking into account the relative humidity in the chamber.

Under normal humidity conditions (95 - 99 % RH), the sensor is unaffected. If for any reason

(such as a large spill inside the chamber or the incubator being switched off while fully humidified) liquid condenses around the sensor, the result may be restriction of gas flow and a low sensor signal. Should such condensation appear on the chamber walls, normal operation can be easily restored by removing the water tray, drying the chamber completely, and then running the incubator at 37 °C for one hour. This will dry out the sensor. After the hour has elapsed, the water tray can be reinstalled and the incubator humidified again.

5.13.3

Setting up oxygen control (1–19 %)

This oxygen control option is designed to cover the 1 – 19 % range by adding nitrogen to bring the level below ambient.

1. Remove the black plastic protective cover from the hydrophobic filter cap (making sure that the hydrophobic filter cap is not removed with it), located in the rear wall of the incubator chamber. Retain the cover for use when you clean the chamber.

2. Open the nitrogen gas supply from the tank. Set the N

2

tank’s outlet pressure gauge to 1.5 bar

3. Set the N

2

inline pressure regulator to 0.1 MPa (1.0 bar, 14.5 psi). The gas flow rate is approximately 20 liters/minute.

If the programmed O

2

level is close to the ambient oxygen, it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value.

4. After the incubator has been humidified and left overnight to stabilize, select the USER menu; then, using the or direction key, select OXYGEN SENSOR-REF TO ATMOSPHERE and follow the onscreen instructions to automatically calibrate the oxygen sensor to atmospheric oxygen levels. The oxygen reading is automatically adjusted to 19.7 %, which is the true reading taking into account the relative humidity level.

48

Operation


English (EN)

Abb. 5-13: Selecting OXYGEN SENSOR-REF TO ATMOSPHERE

Fig. 5-13: Selecting OXYGEN SENSOR-REF TO ATMOSPHERE

5. Enable the oxygen control:

1. Press the USER function key

2. Using the or direction key, select MANUAL DISABLE

3. Press the ENTER function key

4. Select ENABLE for Oxygen Control using the or direction key

5. Press the ENTER function key

Abb. 5-14: USER SETTINGS screen

Fig. 5-14: USER SETTINGS screen

6. Navigate to the PROG screen to set the required oxygen level.

7. The alarm levels are automatically set to ± 0.5 % above or below the programmed value, but you can modify them in the ALARM screen. Re-arming the alarm can be delayed until the programmed value is achieved: select the relevant option in the ALARM screen.

At low oxygen levels, the CO

2

and O

2

levels may not have fully recovered within the Alarm limits after the 15-minute “Delay in arming after door opening.” This time period can be increased (in the ALARM screen) to suit individual circumstances.

8. By controlling the duty cycle of the N

2

valve, oxygen control can be tailored to achieve programmed oxygen and carbon dioxide levels at approximately the same time.

Operation


English (EN)

49

We recommend that you repeat the OXYGEN SENSOR-REF TO ATMOSPHERE

procedure in step 4 (see

Referencing to atmosphere on p. 49) , and further details once a month to ensure that any long-term drift in

sensor output will be corrected. Be sure to do it at the chamber operating temperature.

When you are cleaning the chamber, be very careful not to wet the oxygen sensor or CO

2

sensor. Never use solvents on the sensor membrane; rather, be sure to cap the hydrophobic filter before you clean. It is good practice to replace the filter each time you clean the incubator chamber, to avoid the possibility of filter contamination.

Under normal relative humidity conditions (95 – 99 %), the oxygen sensor’s performance should not be affected. If, however, liquid condenses around the sensor, gas flow may become restricted, giving the sensor a low signal. This may occur if there is a large liquid spill inside the chamber or if the incubator is turned off while it is fully humidified. Should such condensation appear, normal operation can be restored by:

1. Removing the water tray,

2. Drying the chamber completely,

3. Running the incubator at 37 °C for one hour.

This will dry out the sensor(s). The water tray can then be reinstalled and the incubator can be safely re-humidified.

5.14

Referencing to atmosphere

The oxygen sensor is a self-powered electrochemical cell with a finite life that is dependent on the ambient oxygen level. A typical lifespan is 1 – 2 years at atmospheric levels. During the sensor's lifespan, the signal produced will slowly degrade until it is ultimately unuseable. For this reason, we recommend that you reference the sensor to atmospheric oxygen levels on a monthly basis.

To reference the sensor to atmosphere, enter the USER menu and select OXYGEN SENSOR-REF TO

ATMOSPHERE .

The procedure has 3 possible outcomes:

1. The test was completely successful, and no further action is needed until the next month's test.

2. The sensor needs to be replaced soon (see Replace sensor soon on p. 50) .

3. The sensor needs to be replaced immediately (see Replace sensor now on p. 50)

.

5.15

Oxygen sensor replacement

If you complete a referencing procedure and the O

2

sensor is not functioning at 100 %, you will receive:

• O

2

REFERENCE OK BUT SENSOR REQUIRES REPLACEMENT SHORTLY

(see Replace sensor soon on p. 50) or

• O

2

REFERENCE FAILED

(see Replace sensor now on p. 50)

if the sensor is no longer functioning.

50

Operation


English (EN)

5.15.1

Replace sensor soon

If the referencing procedure was successful but the sensor is nearing the end of its working life, the following message will appear in the display:

O

2

REFERENCE OK BUT SENSOR REQUIRES REPLACEMENT SHORTLY

PRESS ENTER TO PROCEED

When you press the ENTER function key, the message will change to this:

O

2

SENSOR

THE RESULT OF THE O

2

REFERENCE PROCESS SHOWS THAT THE SIGNAL FROM THE O

2

SENSOR HAS

REDUCED INDICATING IT IS APPROACHING THE END OF ITS LIFE .

REPEAT THE REFERENCE PROCEDURE TO CONFIRM THIS RESULT .

PRESS ENTER TO PROCEED .

Press the ENTER function key.

5.15.2

Replace sensor now

If the referencing procedure failed, oxygen control will be disabled. The incubator will appear to be functioning as normal until a new sensor is installed and referenced to atmospheric level. The following message appears on the display:

O

2

REFERENCE FAILED

PRESS ENTER TO PROCEED

When you press the ENTER function key, the message will change to this:

O

2

SENSOR

THE RESULT OF THE O

2

REFERENCE PROCESS SHOWS THAT THE SIGNAL FROM THE O

2

SENSOR HAS

REDUCED BELOW AN ACCEPTABLE LEVEL AND HAS REACHED THE END OF ITS LIFE.

REPEAT THE REFERENCE PROCEDURE TO CONFIRM THIS RESULT.

PRESS NEXT TO PROCEED.

When you press the NEXT function key, the message will change to this:

O

2

SENSOR

Operation


English (EN)

51

OXYGEN CONTROL HAS BEEN DISABLED AS A RESULT BUT THE INCUBATOR IS OTHERWISE FULLY

OPERATIONAL.

PRESS PREV TO VIEW PREVIOUS SCREEN. PRESS EXIT TO EXIT.

When you press the EXIT function key, you will return to the USER screen and normal operation.

5.15.3

Removing and replacing O

2

sensor

Prerequisites

• Oxygen sensor removal tool (see Fig. 5-16 on p. 52)


 Grasp white connector body when disconnecting the wire leads.

 Do not pull on the wire leads.

To remove and replace the oxygen sensor, you will need the sensor removal tool provided (see Fig. 5-16 on p. 52).

1. Pull the rear access cover off the rear outside wall of the incubator to gain access to the oxygen sensor.

Abb. 5-15: Oxygen sensor rear access cover

Fig. 5-15: Oxygen sensor rear access cover

2. Reach inside and disconnect the sensor by unplugging the connector: be sure to grasp the white connector body.

3. Using the sensor removal tool (see Fig. 5-16 on p. 52), unscrew the oxygen sensor by turning it

counter-clockwise. Because the oxygen sensor contains lead, be sure to dispose of it according to local regulations.

52

Operation


English (EN)

Abb. 5-16: Oxygen sensor removal tool

Fig. 5-16: Oxygen sensor removal tool

4. If you want to do an HTD, stop the procedure after this step. During the HTD the white connector should be outside the device as shown. Carefully put the rear access cover back in place. Make sure that the cables are not damaged.

Abb. 5-17: Oxygen sensor rear access cover

Fig. 5-17: Oxygen sensor rear access cover

5. Using the sensor removal tool, install the new oxygen sensor by turning it clockwise until it is finger-tight. Do not use excessive force or any metal tool.

6. Reconnect the sensor by plugging the white connector body back in.

7. Make sure that the sensor wires are inside the metal sensor tube to protect them from damage, then press the rear access cover snugly back in place.

8. After replacing the sensor, humidify the incubator and allow it to stabilize overnight.

9. Calibrate the sensor with reference to the atmospheric oxygen level (see Referencing to atmosphere on p. 49) .

Operation


English (EN)

53

5.16

Replacing the filter disc

The hydrophobic filter installed on your oxygen control system helps prevent condensation from reaching the sensor.

1. Carefully pull the complete hydrophobic filter holder away from the oxygen sensor holder on the rear wall of the chamber.

2. With a fingertip or a 10 - 11 mm (½ in) rod, from the rear of the filter holder, gently push the filter membrane disc and the filter cap out of the holder.

3. Clean and dry the filter holder and cap.

4. Wearing gloves to avoid contaminating the filter disc, gently place the new filter membrane disc into the filter holder recess. The filter disc works in both directions, so there is no right or wrong side.

Abb. 5-18: Hydrophobic filter and holder assembly (exploded view)

1 2 3

5

Fig. 5-18: Hydrophobic filter and holder assembly (exploded view)

1 Oxygen sensor holder

2 Hydrophobic filter disc

3 Hydrophobic filter holder

4

4 Hydrophobic filter cap

5 Oxygen sensor

5. Make sure the O-rings (between the cap & filter holder and between the filter holder & oxygen sensor holder) are undamaged and securely in place.

6. Gently press the filter cap back in.

7. Press fit the filter assembly back onto the oxygen sensor holder.

54

Operation


English (EN)

5.17

Humidity alert and monitoring package

The humidity alert package includes a water tray warning system and humidity display and alarm function, both providing optimal feedback on relative humidity inside the incubator chamber.

5.17.1

Water tray warning system

The water tray warning system is designed to warn you, if the water level in the water tray is too low. The water level is continuously measured by an optical water level sensor. If the water level in the water tray is too low a HUMIDITY WATER LOW ALARM is displayed. This alarm can be cancelled by pressing any key, but it will re-activate when the door is opened and closed.

The humidity alarm system can be deactivated:

NOTICE! Risk of damage to equipment

The humidity warning sensor can not be dismantled.

 Never bend the metal tube which came from the back side of the chamber. Just flip the downward part (with the sensor) with the rubber tube to take out the water tray.

1. Press USER .

2. Select DISABLE and toggle HUMIDITY WARNING from ON to OFF using the keys.

3. Press ENTER .

4. Refill the water tray with 0.5 Liters of warm (~37.0 °C) distilled water.

When the water level has been replenished and the door is closed the system will re-arm.

5.17.2

Humidity display and alarm system

The humidity display and alarm system measures and displays the relative humidity level (rH) of the chamber. Under normal operating conditions and using the supplied water tray, the rH level will reach a maximum of 95 - 96 % after being left overnight. The humidity sensor will activate an alarm if relative humidity falls below the preset limit of 88 %. The sensor is located on the rear wall of the chamber, below the CO

2

sensor. The humidity display is always active, but the alarm system can be deactivated:

1. Press USER .

2. Select DISABLE , and toggle RELATIVE HUMIDITY from ON to OFF using the keys.

3. Press ENTER .

The alarm system is disarmed for 1 hour or when it achieves 88 % rH, when the incubator is switched on, or if the door is opened and closed.

An alarm is signaled by a flashing RH LOW ALARM message. If the humidity level rises above 88 % during the 1 hour time-out period, the humidity alarm system will be armed. The alarm will then be triggered, when the rH level falls below 88 % and will be recorded by the alarm log. The alarm can be acknowledged

Operation


English (EN)

55 by pressing any key. No further alarms will occur unless the door is opened and closed, or power to the incubator is removed then restored.

The humidity sensor is protected by a white porous plastic cover. Do not spill any liquid into the sensor. The white porous cover should be protected with the black plastic sleeve provided when cleaning the incubator.

The white porous cover can be removed and autoclaved. Do not touch or wet the sensor when the cover is removed.

5.18

RS-232 interface

An external computer can be connected to the serial interface for data logging using optional BioCommand

SFI software, or by remote control with a communication program. Data from the incubator can be downloaded for record keeping or validation documentation. The device can be controlled remotely and operating parameters can be transmitted and recorded using third party software. Information on installing and operating the communication program can be found in the corresponding software documentation.

The RS-232 interface port provides a connection for the incubator to a personal computer or terminal. This allows the incubator’s operating status to be viewed on the screen.

Only connect devices that meet the safety requirements defined in IEC 60950-1.

The computer should be connected to a mains/power supply outlet as close as possible to the incubator.

Prerequisites

• A PC or terminal, capable of RS-232 communication, with one serial com port free for this connection or a serial interface box.

• A null-modem screened cable of suitable length. To ensure reliable communication, the cable should not exceed 15 m (49 ft) in length. One end must have a 9-pin female D connector, to connect to the incubator’s RS-232 port, and the other end must have either a 9-pin or 25-pin female D connector, whichever mates to your PC or terminal.

• Suitable communications software, such as Eppendorf's BioCommand SFI or HyperTerminal (included with Windows ® NT, 95, 98, ME, 2000 and XP).

The incubator requires the following settings in the communications software:

Tab. 5-3: Communication settings

Baud Rate

Data Bits

19200 bits/second

8

Parity

Stop Bits

Flow Control

None

1

Hardware (some cables may require it to be “None”)

To use the RS-232 interface:

56

Operation


English (EN)

1. Connect the null-modem cable to the RS-232 port at the rear of the incubator.

2. Connect the other end of the cable to either the 25-pin male D serial com port or the 9-pin male D serial com port on the PC or terminal.

3. Turn on the computer and start the communications software.

4. Select an unused serial port to enable communications between the incubator and the PC or terminal.

5. Using the communications software, select the unused port (COM1, COM2, COM3, etc.) then press the

ENTER key on the PC/terminal keyboard (refer to BioCommand SFI operating manual for instructions).

If you have successfully chosen an unused port, the message Type ?

for help should appear on the computer screen.

6. If you see any other message, or nothing happens, select the next port and press the ENTER key again.

If you still do not see the expected message, change the Flow Control setting from Hardware to None , then try again.

7. When the connection between the incubator and PC/terminal has been successfully established, and the message indicated in Step 5 appears, type (as prompted by the message onscreen) a ?

in the communications software, then press the ENTER key.

8. A menu will appear on the computer screen:

The menu allows the incubator temperature, CO

2

and alarm setpoints to be programmed remotely. A running status report can be generated at user-defined intervals, and all reports can be sent to a printer. For details on these features, consult the documentation supplied with your communications software.

Remote programming: all commands beginning with P can be used to program the incubator from the remote computer. These commands are not case sensitive: lower case or upper case letters work exactly the same way. The following are examples of how each of the above commands might be used:

Operation


English (EN)

57

Tab. 5-4: Remote programming

PTnn.n

(P)rogram new (T)emperature setpoint. Type PT37.5

, then press reprogram the incubator’s temperature setpoint to 37.5 °C.

ENTER to

PCnn.n

PATHnn.n

PATLnn.n

(P)rogram new (C)O

2

level. Type PC04.0

, then press ENTER to reprogram the incubator’s CO

2

level to 4 %.

(P)rogram new (A)larm (T)emperature (H)igh setpoint. Type PATH38.0

, then press ENTER to reprogram the incubator’s high temperature alarm setpoint to

38.0 °C.

(P)rogram new (A)larm (T)emperature (L)ow setpoint. Type PATL36.0

, then press ENTER to reprogram the incubator’s low temperature alarm setpoint to

36.0 °C.

PACHnn.n

PACLnn.n

(P)rogram new (A)larm (C)O

2

level (H)igh setpoint. Type PACH05.5

, then press

ENTER to reprogram the incubator’s high CO

2

level alarm setpoint to 5.5 %.

(P)rogram new (A)larm (C)O

2

(L)ow setpoint. Type PACL04.5

, then press ENTER to reprogram the incubator’s low CO

2

level alarm setpoint to 4.5 %.

In each case, the change is confirmed by a message from the incubator (e.g., Program Temperature 37.5 °C

OK or Program Alarm Temperature High 38.0 °C OK etc.).

Status commands : all commands beginning with S can be used to query the incubator from the remote computer, and to display the current status of the incubator. As with the program commands, these commands are not case sensitive: lower case or upper case letters work exactly the same way. The following are examples of how each of the status commands might be used:

Tab. 5-5: Status commands

S (S)tatus report. Type report:

S , then press the ENTER

Temperature: Actual 37.0 °C Setpoint 37.0 °C

CO

2

: Actual 05.0 % Setpoint 5.0 %

key to display a current status

SInn.nn

(S)tatus report at (I)nterval of n minutes, n seconds. Type SI60.0

, then press the

ENTER key to display a current status report (as shown above) every hour. A confirmation message appears:

Status report at interval 60.0 will be given

Press “Enter” or “ESC” to stop reports

Other commands : As with the Program and Status commands, the V (Version number report) and ? (Help) commands are not case sensitive. To use these two commands:

Tab. 5-6: Other commands

V Type V , then press the ENTER key to generate a report on the current firmware version.

? Type key to return to the initial help screen.

Alarm/Event Messages : a number of messages are preset to appear on the computer screen to inform you of either an alarm condition or a certain event. The following table recaps those messages:

58

Operation


English (EN)

Tab. 5-7: Messages

Type of Message

Alarm

Alarm

Alarm

Alarm

Alarm

Alarm (Auto-zero)

Auto-zero Event

Door Event

Door Event

Message

TEMPERATURE LOW

TEMPERATURE HIGH

CO

2

LOW

CO

2

HIGH

TEMPERATURE SENSOR FAILURE

PROG CO

2

A/Z SYSTEM INOPERATIVE

PROG CO

2

A/Z COMPLETED OK

DOOR OPENED

DOOR CLOSED

After displaying any of the above messages, a status report message relative to the alarm or event will be displayed, e.g.:

[DOOR EVENT] DOOR OPENED

Temperature: Actual 37.0 °C Setpoint 37.0 °C

CO

2

: Actual 00.1 % Setpoint 05.0 %

Troubleshooting


English (EN)

59

6

6.1

Troubleshooting

General errors

If the suggested troubleshooting measures fail repeatedly, contact your local Eppendorf partner. You can find the addresses on the webpage www.eppendorf.com.

Sample is partially dry Shelves are not properly leveled.

Solution

Level the incubator by placing a spirit level on a shelf:

• aligned left to right

• aligned front to rear

Sample evaporation

Circular cell growth in cell culture dish

Sample contaminated

CO

2

pressure below warning level

Add more medium to the cells.

No water in the water tray.

Check the water tray weekly.

Refill or clean and refill the water tray weekly.

Low humidity due to empty water tray.

Vibrations from other devices result in sample vibrations.

Consistent contamination

Contamination of sample

Check the water tray weekly.

Refill or clean and refill the water tray weekly.

Check if vibration generating elevators or devices like freezers or centrifuges are too close to the incubator, see Installation .

Perform a high-temperature disinfection.

CO

2

cylinder is almost empty.

Check your sample handling.

Check for contamination during your application steps.

Check CO

2 supply. Exchange CO

2

cylinder and adjust gas supply.

Check gas connection on disconnection or leakage, e.g., in-line gas filter, gas connection.

6.2

O

2

sensor

If the oxygen sensor fails suddenly, it is very likely that the sensor inlet membrane has become blocked by condensation. This can be seen on the DATALOGGER screen as a sudden drop from the programmed value to nearly zero.

To dry the membrane:

1. Remove the water tray.

2. Remove the hydrophobic filter holder assembly (see Replacing the filter disc on p. 53) by unscrewing it

(counter-clockwise).

3. Program the incubator for a temperature of at least 37 °C (or higher if you normally operate the incubator at a higher temperature).

4. Close the door and allow the temperature to recover.

60

Troubleshooting


English (EN)

5. Reopen the door for 15 seconds to release any build-up of humidity.

6. Repeat steps 3 and 4 every 30 minutes while monitoring the DATALOGGER O

2

Graph. The oxygen level should recover after a few hours.

7. Leave the incubator for a few more hours to be ensure that the sensor membrane has thoroughly dried out.

8. Replace the hydrophobic filter disc (see Replacing the filter disc on p. 53) .

9. Re-humidify the incubator.

10. After 2 to 3 hours, carry out an OXYGEN SENSOR-REF TO ATMOSPHERE

(see Referencing to atmosphere on p. 49)

. When the referencing has been successfully completed, the incubator is ready for use.

Maintenance


English (EN)

61

7

7.1

Maintenance

Routine maintenance

7.1.1

General

WARNING! Risk of injury to personnel or damage to equipment!

 2 stacked incubators should not be moved for maintenance or cleaning.

To ensure that chamber conditions remain stable, minimize the length of time that the door is open. The magnetic door catches are specifically designed to make door opening and closing as easy as possible.

When you open the door, wipe off any small drops of condensate that may have formed on the inner seal.

This will avoid a build-up of condensation.

If you are using the water tray for humidification, (see Using the water tray on p. 30) .

7.1.2

Daily checks

1. Check that the temperature and CO

2

levels are reading within specification.

2. Check the reserve pressure in the CO

2

cylinder (normally 725 PSI (50 bar) when full). The design of the incubator ensures very low consumption of CO

2

. During normal working conditions, a typical large cylinder should last approximately 12 months (frequent door openings will deplete the supply more rapidly). If there is a significant drop at the cylinder pressure of 725 PSI (50 bar), it means that the cylinder is almost empty and should be replaced. Ensuring that there are no leaks at any of the connections will ensure a greater lifetime to the CO

2

supply and will help avoid accidentally running out of CO

2

.

3. Any spills in the chamber should be cleaned immediately.

4. Check the DATALOGGER screen for any alarms or events that may have occurred overnight.

7.1.3

Weekly checks

Use distilled water only in the water tray. Use of any other types of water including deionized water and chlorinated additives will cause corrosion inside the incubator.

Refill the water tray with 0.5 liters of distilled water. The use of warm water will ensure a rapid return to optimum chamber conditions.

7.1.4

Monthly checks

We recommend routine replacement of the water in the water tray, and that you clean the tray at the same time.

If required, you can take a sample of the gas inside the chamber using the CO

2

sample port, and check it using a CO

2

gas analyzer (see Accessories on p. 73)

.

62

Maintenance


English (EN)

Displayed chamber CO

2

level will drop during sampling, but it will recover once the sampling is complete. This is merely a sensor characteristic; the CO

2

level in the chamber is actually not affected.

We recommend that you perform a CO

2

autozero prior to sampling. We also recommend that you autozero the CO

2

system at least once every 28 days to ensure that CO

2

level is correct.

7.1.5

CO

2

sampling with analyzer

The CO

2

sample port is located on the rear of the incubator, near the top (see Fig. 3-1 on p. 15).

If you conduct a sampling, please ensure the following:

• Turn off the CO

2

gas by re-programming the setpoint for CO

2

to 0.0 % to prevent CO

2

from being injected into the chamber and giving a false reading.

• A flow rate ≤ 0.5 liters/minute is used to take a sample.

• The door is kept closed.

• Reset the CO

2

setpoint to the desired level after sampling.

We recommend that you perform a CO

2

autozero prior to sampling. We also recommend that you autozero the CO

2

system at least once every 28 days to ensure that CO

2

level is correct.

7.2

Cleaning

DANGER! Electric shock.

 Switch off the device and disconnect the mains/power plug before commencing any service or cleaning operations.

WARNING! Risk of injury to personnel or damage to equipment

 While cleaning or disinfecting/decontaminating the chamber, take care not to break small sensors/other parts or destroy your protective gloves by wiping with too much power.

WARNING! Risk of injury to personnel

 While cleaning, disinfecting/decontaminating or preparing the chamber, take care not to damage your protective gloves at small sensor parts extending into the chamber.

NOTICE! Corrosion due to aggressive cleaning agents and disinfectants.

 Do not use any corrosive cleaning agents, aggressive solvents or abrasive polishes.

 Do not incubate the accessories in aggressive cleaning agents or disinfectants for longer periods.

Maintenance


English (EN)

63

1. Routinely clean the exterior of the device by wiping it over with a soft cloth, moistened with soapy water.

2. Rinse the soap from the cloth in clean water, and wipe the exterior surfaces again.

7.3

Disinfection/Decontamination

DANGER! Danger from exposure to decontamination agents

 Wear appropriate laboratory clothing, protective cloves and safety glasses.

 Wear breathing protection if you work with particulate matter.

CAUTION! Risk of potential harm to personnel

 As a routine precaution, wear protective gloves.

 Be sure to adequately ventilate the work area as you disinfect, to avoid the formation of potentially explosive alcohol vapors.


 Never use any of the following substances to clean the stainless steel, or damage will result: Sodium Azide, Aqua Regia, Iodine, Ferric Chloride, Sulphuric Acid or Chlorine based solvents.


 It is very important to ensure that no liquid is spilled onto the white porous CO

2

sensor cover at the rear of the chamber. Failure to use the protective cover(s) could result in damage to the sensor(s) and may affect your warranty.

Use the decontamination method recommended by the manufacturer.

If you would like to use a different method, contact Eppendorf AG to prevent the device from becoming damaged.

If you have any further questions regarding the cleaning and disinfection or decontamination and the cleaning agents to be used, contact Eppendorf AG.

The contact details are provided on the back of this manual.

The recommended disinfecting agent for use with the incubator is a solution of 70 % isopropanol

(isopropyl alcohol) and 30 % distilled water. Be sure to follow appropriate safety regulations while you are using this solution.

1. Program 0.0 % CO

2

and switch off the incubator. Unplug the incubator from the mains/power supply.

2. Dampen a clean cloth with the alcohol solution and wipe down all external surfaces, taking care to keep the alcohol solution from coming into contact with any mains/electrical outlets or assemblies.

3. Remove all of the shelves, the water tray, and the shelf racks.

64

Maintenance


English (EN)

4. Place the black protective cover over the CO

2

sensor. Also protect any additional sensors, such as

Oxygen or Humidity, with the cover(s) supplied.

5. Clean the water tray by rinsing it in sterile water, wiping it down with the alcohol solution, and then rinsing it again with sterile water.

6. Wipe down the inside of the chamber with the alcohol/water solution, and leave it to dry completely.

7. Wipe the internal components of the chamber twice with the alcohol/water solution. Wipe off excess liquid and leave it to dry completely.

8. Reassemble the shelf racks, shelves, and water tray before switching the incubator on. Wipe the inner door seal with the alcohol solution, rinse and leave it to dry.

9. Ensure the protective covers are removed from all sensors. Keep them aside. Be very careful, as you remove the black CO

2

sensor cover, not to accidentally remove the white porous sensor cover. The white cover must remain in place.

10. O

2

option: Check if the hydrophobic filter disc and the hydrophobic filter cap of the O

2

sensor are at their place.

11. Refill the water tray (see Using the water tray on p. 30) . When reinstalling, ensure that the water tray is

pushed fully back.

12. Leave the incubator on for at least two hours (preferably overnight) to allow conditions to stabilize.

13. When the incubator has stabilized, carry out an Auto-zero and reprogram the desired CO

2

level. It may be necessary to open the inner door briefly if the CO

2

level is too high after performing an Auto-zero.

7.4

High-temperature disinfection

If your incubator is supplied with the High-temperature disinfection option, follow the guidelines outlined

(see High-temperature disinfection on p. 41)

for information on how to correctly and safely operate this option.

7.5

Shipping the device

WARNING! Risk of personal injury due to contamination.

People may become contaminated if you store or ship a contaminated device.

 Clean and decontaminate the device before shipping or storage.

NOTICE! Risk of damage due to improper packaging.

Eppendorf AG is not liable for any damage caused by improper packaging.

 Only store and transport the device in its original packaging.

 If you do not have the original packaging, request original packing from Eppendorf AG.

Maintenance


English (EN)

65

Prerequisites

• The device has been cleaned and decontaminated.

• The original packaging is available.

1. Download the "Decontamination declaration for product returns" from www.eppendorf.com.

2. Complete the decontamination declaration.

3. Pack the device.

4. Put the decontamination declaration into the packaging.

Deliveries shipped without a decontamination certificate will not be processed.

5. Send the device to Eppendorf AG or an authorized service center.

66

Maintenance


English (EN)

Transport, storage and disposal


English (EN)

67

8

8.1


Transport

 Use the original packing for transport.

General transport

Air freight

Air temperature

-25 °C – 55 °C

Relative humidity

10 % – 95 %, non-condensing

Atmospheric pressure

0.7 bar (70 kPa) –

1.06 bar (106 kPa)

-25 °C – 55 °C 10 % – 95 %, non-condensing

0.7 bar (70 kPa) –

1.06 bar (106 kPa)

8.2

Storage

In transport packing

Without transport packing

Air temperature

-25 °C – 55 °C

-25 °C – 55 °C

Relative humidity



Atmospheric pressure

0.7 bar (70 kPa) –

1.06 bar (106 kPa)

0.7 bar (70 kPa) –

1.06 bar (106 kPa)

8.3

Disposal

If the product needs to be disposed of, the relevant legal regulations must be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2012/19/EU pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To document this, they have been marked with the following marking:

Because disposal regulations may differ from one country to another within the EU, please contact your supplier if necessary.

68



English (EN)

Technical data


English (EN)

69

9

9.1

Technical data

Weight/dimensions

9.1.1

Equipment dimensions

Width

Height

Depth

Net weight

9.1.2

Internal dimensions

Width

Height

Depth

Volume

9.1.3

Transporting dimensions

Width

Height

Depth

Weight

9.1.4

Shelves

Polished stainless steel, perforated (standard).

Usable area

Number of shelves

48.9 cm (19.3 in)

65.2 cm (25.7 in)

49.7 cm (19.6 in)

31.8 kg (70.1 lb)

40.1 cm (15.8 in)

40.1 cm (15.8 in)

30.5 cm (12.0 in)

48 liters (12.7 gallons)

70.0 cm (27.6 in), pallet included



50 kg (110.2 lb)

875 cm² (344.5 in²)

• 3 shelves as standard, coming with rack for 6 shelves

• Possibility of upgrade by ordering additional shelves

70

Technical data


English (EN)

9.2

Power supply

Mains/power connection 110 V – 127 V ±10 %, 50 Hz – 60 Hz

220 V – 240 V ±10 %, 50 Hz – 60 Hz

500 W Power consumption for 110 V – 127 V

Power consumption for 220 V – 240 V

Energy to maintain 37 °C

Overvoltage category

Protection class

1000 W

< 0.1 kWh

II (IEC 61010-1)

I

Max. power consumption during normal application

110 V – 127 V

220 V – 240 V

250 W

400 W

Max. power consumption during high temperature disinfection

110 V – 127 V

220 V – 240 V

500 W

1000 W

9.3

Ambient conditions

Ambient temperature

Storage temperature

Altitude limit

Relative humidity

Pollution degree

15 °C – 28 °C

10 °C – 50 °C

2000 m

20 % – 80 %

2

9.4

Specifications

9.4.1

Temperature management

• Measurements of chamber and door temperatures are taken by 4 RT (resistance temperature) curve matched sensors with a sensitivity of 0.01 °C.

• The door heating element features an adjustable independent control.

• Out of Limits temperature protection system is independent of the microprocessor control.

Range

Control increment

Stability

Uniformity

4 °C above ambient temperature to 50 °C

0.1 °C

± 0.1 °C at 37 °C

± 0.3 °C at ambient 20–25 °C

If the ambient temperature is close to the programmed value, control settings may need adjusting. Consult Eppendorf Service for instructions.

Technical data


English (EN)

71

9.4.2

CO

2

control

Solid-state infrared CO

2

sensor is operating independently of humidity. The incubator has a programmable, fully automatic zeroing function.

Range

Control increment

Stability

Uniformity

Gas connections

Required gas pressure

9.4.3

Humidity

0.2–20 %

0.1 %

± 0.2 % at 5 % CO

2

± 0.1 %

OD 10 mm and ID 6.5 mm

0.05 MPa (0.5 bar/7.2 psi)

Water tray capacity

Humidity control: manual

0.5 L

95 % at 37 °C

The relative humidity attained could vary in the range of 90 % ± 5 % depending on ambient humidity level and other factors.

9.4.4

Calibration

Maximum operating ambient temperature is 28 °C.

Factory calibration of the incubator is carried out at 37 °C, 5 % CO

2

and 90–95 RH, in an ambient temperature of 20–25 °C with no heat-generating apparatus inside the chamber.

Software calibration adjustments are required to optimize performance, if the incubator is being used outside the operating conditions. Performance specifications are also affected.

Contact Eppendorf Service for advice on calibration adjustments and relevant performance specifications.

Be prepared with the model and serial number of your incubator and the complete details of your operating conditions.

Performance values represent the average of instruments checked in the factory under optimum conditions.

72

Technical data


English (EN)

9.5

Oxygen sensor

Sensor type

Zero signal in Nitrogen

Temperature compensation

Relative humidity range

Operating temperature range

Self-powered, diffusion-limited, electrochemical cell with temperature compensation

< 50 μV

±2 % of signal variation from 0–40 °C

0–99 %, non-condensing

-20 °C to +50 °C

Resolution

Expected operating life

0.01 % oxygen

1–2 years in ambient oxygen

Nitrogen input rate 20 L/min at 0.1 MPa (1.0 bar/14.5 psi)

Typical oxygen reduction rates 3 minutes to 16 %

4 minutes to 11 %

8 minutes to 6 %

Ordering information


English (EN)

73

10 Ordering information

10.1

Accessories

P0628-5030

P0628-6150

P0628-6831

P0628-5060

P0628-5920

P0628-5070

P0628-5080

P0628-5090

P0628-5100

P0460-7750

P0460-7751

P0620-7012

M1291-1001

Order No.

(International)

P0628-5000

P0628-5010

6731 070.107

Description

CO

2

cylinder auto-changeover controller

Two-stage CO

2

pressure regulator

Gas tubing

3 m, with in-inline gas filter

CO

2

in-line pressure regulator

Electronic CO

2

gas analyzer

Electronic CO

2

and O

2

gas analyzer

Auto-zero filter

Hydrophobic filter for O

2

sensor

Non-perforated shelves

Perforated shelves

Lower stacking frame with castors

Shelf racks (for max. 6 positions)

8-port RS-232/485 to USB converter

4-port RS-232/485 to USB converter

CO

2

incubator cable, 15 ft.

BioCommand ® SFI package for incubator

For information regarding accessories not listed, contact a customer service representative.

Quantity

1

1

1

1

1

1

1

1

1

1

1

Pack of 5

10.2

Available options for device

Some option combinations are not possible, others may incur extra cost. Please inquire before ordering.

• O

2

Control, 1 – 19 %

• O

2

Control, 0.1 – 19 %

• High-temperature disinfection

• Humidity alert and monitoring package

• Split inner door

• Upper stacking frame

• Lower and upper stacking frame

• Under-bench stand, 200 mm high with feet

74

Ordering information


English (EN)

10.3

Connectivity

Order no.

(International)

1006 000.009

1006 071.003

1006 072.000

1006 076.005

Description

VisioNize box with mains/power adapter

Cable

USB to RS232 adapter, 5 m

Mains/power adapter for VisioNize box

VisioNize box adapter for incubator S41i

Index


English (EN)

75

Index

A

Access code, programming ...................................31

Alarm events..........................................................35

ATEX Guideline .....................................................12

B

BMS relay contact alarm .......................................44

C

Caution, explanation of ...........................................7

Chamber alarm system function ...........................39

P

Power frequency ................................................... 34

Programming desired O2 level ............................. 31

R

Removing access code.......................................... 31

Removing and replacing O2 sensor...................... 51

Replace sensor now .............................................. 50

Replace sensor soon ............................................. 50

Replacing filter disc .............................................. 53

Requirements, utilities.......................................... 22

Routine maintenance ............................................ 61

RS-232 interface ................................................... 55

D

Daily checks ..........................................................61

Danger, explanation of ............................................7

Datalogger ............................................................35

Direct heating system............................................19

Disinfection ...........................................................34

Disposal .................................................................67

S

Seamless chamber ................................................ 19

Setting date and time............................................ 32

Stacking devices ................................................... 20

Standard features.................................................. 19

Storage.................................................................. 67

H

Hazard symbols .......................................................7

High temperature disinfection ........................41, 64

High temperature disinfection with O2 control ....44

T

Troubleshooting the O2 sensor ............................ 59

Two-level alarm system ........................................ 20

M

Monthly check .......................................................61

Multiple options ....................................................20

U

User settings ......................................................... 32

Utilities requirements ........................................... 22

N

Notice, explanation of .............................................7

W

Warning, explanation of ......................................... 7

Water tray ....................................................... 19, 30

Weekly checks ...................................................... 61

O

Operating controls.................................................19

76

Index


English (EN)

Certificates

Evaluate Your Manual

Give us your feedback.

www.eppendorf.com/manualfeedback

Your local distributor: www.eppendorf.com/contact

Eppendorf AG · Barkhausenweg 1 · 22339 Hamburg · Germany [email protected] · www.eppendorf.com

No results