Magus Bio VD300 LCD Biological Inverted Digital Microscope User Manual

M AGUS B I O V D 3 00 LCD
BI OLOGICAL INVERT ED D I G I TA L MI CROS COPE
USER MANUAL
Before using the microscope, please read this user manual carefully to
study the instrument design, operation modes and procedures, operational
limitations, and safety precautions.
Due to the continuous improvements in the microscope design, this manual
may not reflect minor design changes that do not affect the microscope
performance and operation procedures.
SAFET Y PRECAUTIONS
MICROSCOPE
1. To avoid electric shock or fire, switch off and unplug the microscope before assembling
the microscope, replacing the bulb or fuse.
2. Do not disassemble the microscope, except for the removable parts specified in this manual.
This can seriously damage its performance. In case of malfunction, please contact a qualified
service center.
3. Make sure that the input voltage of the microscope matches that of the local power supply.
Using the power supply with the wrong input voltage may cause a short circuit or fire.
4. Using an incorrect bulb, fuse, or power cord may damage the microscope or cause a fire.
The power cord must be grounded reliably.
5. In order to avoid a short circuit or any other malfunction, do not expose the microscope to high
temperatures or humid or moist environments for a long period of time.
6. If water splashes on the microscope, immediately switch the power off, unplug the power
cord, and wipe off the water with a dry cloth.
7. The microscope light bulb generates high temperatures during operation. To avoid burns,
do not touch the collector lens or the bulb itself for 10 minutes after the lights have been
switched off. To prevent fire, do not place paper or flammable or explosive materials near
the air vents on the underside of the base.
8. The microscope employs a coaxial coarse/fine focusing mechanism. Do not turn the left/right
coarse/fine focusing knobs in opposite directions. When the limit is reached, you should no
longer rotate the coarse focusing knob.
9. Do not expose the microscope to direct sunlight or other light sources. Do not expose the
microscope to high temperatures, humidity, or dust; otherwise, it may cause condensation,
mold growth, or contamination of the optical parts.
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10. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning
solution to keep the lenses clean.
11. Bulb installation. This microscope employs LED bulbs as a light source. The bulbs should
be replaced by the equipment vendor or in a qualified service center. If you replace the LED
yourself, the illumination function may be impaired.
CAMERA
1. Never view the sun, another bright source of light or a laser through a camera - THIS IS
DANGEROUS FOR YOUR EYESIGHT!
2. Do not disassemble the camera yourself.
3. Keep the camera away from moisture and do not use it in the rain.
4. Protect the camera from shocks, excessive stress from other objects.
5. Store the camera away from corrosive environments, household and car heaters, switched-on
light bulbs and open flames.
6. If there is dirt on the optical surfaces, first blow off dust and small particles or brush them off
with a soft brush, then clean the surface with a soft, clean cloth moistened with alcohol
or ether.
7. If any instrument part or power component has been swallowed, seek medical attention
immediately.
MONITOR
1. Make sure that the input voltage of the monitors matches that of the local power supply. Using
the power supply with the wrong input voltage may cause a short circuit or fire.
2. Do not use the damaged power source.
3. Do not use the damaged power cord.
4. Do not insert foreign objects into the slot of the monitor body.
5. Do not expose the monitor to high temperatures or humidity for a long time.
6. If water splashes on the monitor, immediately switch the power off, unplug the power cord,
and wipe off the water with a dry cloth.
7. Protect the monitor from shocks, excessive stress from other objects.
8. Store the monitor away from corrosive environments, household and car heaters, switched-on
light bulbs and open flames.
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CONTENTS
1 DESCRIPTION OF THE MICROSCOPE
5
Purpose
5
Specifications
5
Microscope kit
7
2 MICROSCOPE PARTS
10
Stand
10
Focusing mechanism
10
Microscope head
10
Eyepieces
11
Revolving nosepiece
11
Objectives
11
Lamp house
11
Stage
12
Camera
12
Monitor
12
3 UNPACKING AND ASSEMBLING THE MICROSCOPE
12
4 BRIGHTFIELD OBSERVATION PROCEDURE
13
Switching on the illumination
13
Adjusting the condenser
14
Adjusting the eyepiece tubes
14
Placing the specimen
14
Focusing on the specimen
15
Adjusting the diopter
16
Observing specimens in tall glassware
16
Calculating the total magnification
17
Calculating the field of view
17
Using the camera
17
Using the monitor
18
5 PHASE-CONTRAST TECHNIQUE
19
6 USING OPTIONAL EQUIPMENT
20
Eyepiece with a scale
Using a calibration slide with a camera
20
20
7 TROUBLESHOOTING
21
8 SCOPE OF DELIVERY
22
9 CARE AND MAINTENANCE
23
Replacing the bulb
23
Replacing the fuse
23
Maintenance
23
10 MAGUS WARRANTY
24
MAGUS Bio VD300 LCD Biological Inverted Digital Microscope has been designed and tested in accordance with
the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property,
and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
1 DESCRIPTION OF THE MICROSCOPE
PURPOSE
The microscope is designed for studying tissue cell cultures, liquid precipitates in special laboratory glassware.
The microscope is intended for observing objects in transmitted light using the brightfield and phase-contrast
techniques. The phase-contrast technique allows for the observation of unstained low-contrast objects, colorless
transparent specimens and living microorganisms.
The microscope illumination system allows for working with glassware up to 70mm high with a bottom thickness
of 1.2mm.
The microscope is used in biomedical laboratories, biotechnology, pharmaceutical research, agriculture, and
environmental studies. The microscope can be used for scientific purposes, for laboratory diagnosis, and for education.
The microscope design allows for capturing and displaying specimen images in real time on the monitor using
a special camera.
SPECIFICATIONS (TABLE 1)
Microscope
Magnification, x
Tube length
Microscope head
100–400 basic configuration (**optional 40–500, 600, 800, 1000)
Infinity (∞)
Trinocular
Eyepiece diameter: 30mm
Siedentopf type
45° inclined
Interpupillary distance: 48–75mm
The light path splitting ratio: 50/50
Eyepieces, x/field, mm
10х/22mm, eye relief: 10mm
10Х/22mm with a scale, the scale division value: 0.1mm*
12.5x/14mm*, 15х/15mm*, 20х/12mm*, 25х/9mm*
Revolving nosepiece
4 objectives
Infinity plan achromatic objectives
Optical design
Objectives, magnification,
x/aperture, working distance
Stage
parfocal distance: 45mm
long focal length
PHP2 PL L 10x/0.25 phase-contrast WD 4.3mm
PL L 10x/0.25 WD 4.3mm
PL L 20х/0.40 WD 8.0mm
PL L 40x/0.60 WD 3.5mm
4x/0.10*; 20х/0.40 phase-contrast*; 40x/0.60 phase-contrast*
Fixed stage
Stage size: 227mm×208mm
Mechanical XY stage attachment. Moving range: 77mm longitudinal, 112mm lateral
Dish holders:
1. 34mm×77.5mm; Ø68.5mm
2. 57mm×82mm; Ø60mm
3. 29mm×77mm; Ø90mm
5
Condenser
Focusing mechanism
Light source
Phase-contrast device
Power supply
Numerical aperture: 0.6
Working distance: 70mm
with an adjustable aperture diaphragm and a slot for the phase-contrast slider
Coaxial coarse & fine focusing knobs on both sides
Fine focusing scale value: 2μm
Coarse focusing lock knob and coarse focusing tension adjusting knob
9W LED with adjustable brightness
Phase-contrast slider with an open slot and a phase slide for the 10x objective
Centering telescope
AC voltage 85–265V, 50/60Hz
Fuse specifications: 250V, 3.0A
Camera
Number of megapixels
2
Sensor
SONY CMOS
Maximum resolution, pix
1920x1080
Color/monochrome
Color
Sensor size
1/1.9" (7.20х4.05mm)
Pixel size, µm
3.75x3.75
Auto focus
+
Light sensitivity
1120mV with 1/30s
Signal/noise ratio
0.15mV with 1/30s
Exposure
Video recording
Frame rate, fps at resolution, pix
0.06 – 918 ms
+
60@1920х1080 (HDMI), 25@1920х1080 (Wi-Fi)
Image format
*.jpg
Video format
*.asf
Spectral range, nm
Shutter type
System requirements
380–650 (IR-filtered)
ERS (electronic rolling shutter)
Windows 8/10/11 (32 and 64 bit), Mac OS X, Linux, up to 2.8 GHz Intel Core 2
or higher, minimum 4GB RAM, USB 2.0 ports, RJ45, CD-ROM, 19" or larger monitor
(with USB connection)
Software
HDMI: built-in, USB: MAGUS View
Mount type
C-mount
Body material
Metal
Power supply
12V/1A AC power adapter
Monitor
Type of matrix
IPS
Screen diagonal, inch
13.3
Screen resolution, pix
1920x1080 (Full HD)
6
Aspect ratio
16:9
Brightness, cd/m2
400
Number of displayed colors
16.7m
Contrast ratio
1000:1
Horizontal/vertical viewing
angle, °
178/178
Viewable screen size (WxH), mm
295x165
Pixel pitch (WxH), mm
0.154x0.154
Display refresh rate, Hz
60
Type of matrix backlight
LED
LED backlight lifetime, h
50000
Interface
HDMI
Power supply
AC 110–220V, DC 5–12V/1A (Type-C)
Power consumption, W
12 (maximum)
Dimensions without package
(WxHxD)
225mm×508mm×563mm
Package dimensions (WxHxD)
427mm×670mm×647mm
Weight
15kg
Weight with package
19kg
* Not included in the kit, available on request.
** The magnification of the microscope can be increased by using additional (optional) eyepieces and objectives.
The manufacturer reserves the right to make changes to the product range and specifications without prior notice.
MICROSCOPE KIT
This microscope kit includes the following main components:
– stand with the built-in power supply, transmitted light source, focusing mechanism, fixed stage with mechanical
XY stage attachment, condenser with phase-contrast slider, and revolving nosepiece
– trinocular microscope head
– set of objectives and eyepieces
– set of dish holders
– digital camera
– monitor
– set of spare parts and accessories
– packaging
– user manual.
See Section 8 of the User manual for a full kit contents.
The general view of the microscope is given in Fig. 1 and 2
7
10
11
12
13
1
9
2
7
3
4
5
8
6
Fig. 1. MAGUS Bio VD300 LCD Microscope. View from the left
1. Eyepieces
2. Microscope head
3. Coarse focusing tension adjusting knob
4. Coarse focusing knob
5. Fine focusing knob
6. ON/OFF switch
7. Stand
8
8. Revolving nosepiece
9. Objectives
10. Illuminator
11. Camera locking screw
12. Trinocular tube with a camera port
13. Interpupillary scale
11
10
15
12
9
13
8
1
14
2
3
7
4
5
6
Fig. 2. MAGUS Bio VD300 LCD Microscope. View from the right
1. Eyepiece tubes
2. Head locking screw
3. Coarse focusing lock knob
4. Coarse focusing knob
5. Fine focusing knob
6. Brightness adjustment ring
7. Object moving knob
8. Stage
9. Aperture diaphragm control
10. Phase-contrast slider
11. Filter
12. Condenser
13. Dish holder
14. Monitor
15. Camera
9
2 MICROSCOPE PARTS
STAND
The stand has stable ergonomic design. Parts attached to the microscope stand 7 (Fig. 1):
– revolving nosepiece 8 (Fig. 1) with objectives
– stage 8 (Fig. 2)
– condenser 12 (Fig. 2)
– lamp house 10 (Fig. 1)
– microscope head 2 (Fig. 1).
Inside the stand is the focusing mechanism and the power supply unit for the lamp house. The power supply converts
AC voltage to the required voltage to power the LED.
There is an ON/OFF switch 6 (Fig. 1) on the left side of the stand. The power is on when the switch is in "–" position.
The power is off when the switch is in "0" position.
There is a ring 6 (Fig. 2) on the right to adjust the supply voltage of the light source.
The back panel of the microscope stand contains a fuse holder and a connector for the AC power cord, which
connects the microscope to an AC outlet.
FOCUSING MECHANISM
The focusing mechanism is located inside the microscope stand. The mechanism has coaxial design – coarse and fine
focusing knobs, coarse focusing tension adjusting knob, and coarse focusing lock knob are mounted on the same axis.
Focusing on the specimen is achieved by adjusting the height of the revolving nosepiece with objectives. Coarse
focusing is performed by rotating the coaxial knobs 4 (Fig. 1, 2) on both sides of the microscope stand.
Fine focusing is performed by rotating the knobs 5 (Fig. 1, 2) on both sides of the microscope stand. Fine focusing
allows for more precise focusing on the specimen and re-focusing the microscope to get an accurate image resolution
when changing objectives and specimens.
The coarse focusing tension adjusting knob 3 (Fig. 1) is the ring between the stand and the coarse focusing knob
on the left side. The ring adjusts the coarse focusing tension so that the tension is comfortable for the user, but the
revolving nosepiece with objectives does not lower spontaneously during operation.
The coarse focusing lock knob 3 (Fig. 2) is located on the right side. Once the coarse focusing is completed, we
recommend rotating the knob clockwise as far as it will go. This secures the coarse focusing position to allow for rapid
re-focusing after the specimen is changed.
Fine focusing scale value: 2μm.
To prevent the focusing mechanism from damage:
– do not turn the left/right coarse/fine focusing knobs in opposite directions
– do not rotate the coarse focusing knob after the knob reaches its limit.
MICROSCOPE HEAD
The trinocular head 2 (Fig. 1) provides the visual observation of the specimen image. The microscope head is installed
in the mounting hole on the top of the microscope stand 7 (Fig. 1) and secured with a clamping screw 2 (Fig. 2).
The interpupillary distance is adjusted by rotating the eyepiece tubes 1 (Fig. 2) in the range of 48–75mm. The distance
between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale 13 (Fig. 1).
For convenience, the microscope head is inclined at 45°.
Microscope head magnification: 1x.
Eyepiece diameter: 30mm.
The eyepiece tubes do not have diopter adjustment to compensate for the observer's ametropia. The diopter
adjustment is on the eyepiece.
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A camera (video-eyepiece) or an imaging system with a monitor is installed in the trinocular tube 12 (Fig. 1)
of the microscope head using a C-mount 1x adapter. The light path splitting ratio: 50% trinocular tube, 50% eyepieces.
EYEPIECES
The microscope kit includes eyepieces 1 (Fig. 1). The eyepieces have high eye relief and are designed to work with
or without glasses.
Eyepiece diameter: 30mm.
Eyepiece magnification: 10x. Field of view: 22mm. Eye relief: 10mm.
There is a diopter adjustment on one of the eyepieces to compensate for the observer's ametropia.
The 10х eyepiece with a scale and 0.1mm scale value, 15х/15mm, 20х/12mm, and 25х/10mm eyepieces are not
included in the kit and are optional.
REVOLVING NOSEPIECE
The revolving nosepiece 8 (Fig. 1) allows for the installation of four objectives. Objectives are changed by rotating
the knurled ring of the revolving nosepiece until the objective fits into place.
Do not rotate the revolving nosepiece by holding the objectives.
The revolving nosepiece rotates clockwise and counter-clockwise.
The revolving nosepiece is installed on the stand under the stage. The objectives are screwed clockwise into
the revolving nosepiece in order of increasing magnification. The objectives are turned ‘away from the observer’.
OBJECTIVES
Objectives 9 (Fig. 1) are designed for the infinity-corrected tube length. The parfocal distance: 45mm. The linear field of
view: 22mm. The objectives have long focal length and are intended to be used with glassware with a bottom thickness
of 1.2mm.
Each objective has the following inscriptions: "PL L" or "PL L PHP2" correction type, linear magnification, numerical
aperture, "∞" tube length, magnification color code according to the international standard.
The specifications of the objectives are given in Table 2:
Objective identification
Microscopy
technique
Magnification
Numerical
aperture
Working
distance, mm
Color
marking
PL 4x/0.10
Brightfield
4х
0.10
21.0
red
Phase-contrast
10х
0.25
4.3
yellow
Brightfield
10х
0.25
4.3
yellow
Phase-contrast
20х
0.40
8.0
green
Brightfield
20х
0.40
8.0
green
Phase-contrast
40х
0.60
3.5
light blue
Brightfield
40х
0.60
3.5
light blue
PL L 10х/0.25 PHP2
PL L 10х/0.25
PL L 20х/0.40 PHP2
PL L 20х/0.40
PL L 40х/0.60 PHP2
PL L 40х/0.60
The 40x objectives have a spring-loaded mount to prevent mechanical damage to the front lens and the object.
If objectives are damaged, we recommend repairing them in the service center.
The objectives are intended to image the specimens through air. Do not use immersion oil.
LAMP HOUSE
The lamp house 10 (Fig. 1) is fixed on the microscope stand. The light source – 9W LED.
The essential part of the lamp house is the condenser. The condenser 12 (Fig. 2) is fixed on the microscope stand.
The condenser allows for working with glassware up to 70mm high.
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The condenser can be used for the brightfield and phase-contrast techniques. The condenser has a slot for the phasecontrast slider. The phase-contrast slider 10 (Fig. 2) has an open slot and a phase slide for the 10x objective.
The iris aperture diaphragm is located under the slot for the phase-contrast slider. The aperture diaphragm is opened
by the knob 9 (Fig. 2). The diaphragm allows setting a specific aperture to work with brightfield objectives. To achieve
greater contrast, you can use the color filters supplied with the microscope. The color filters 11 (Fig. 2) are mounted
in the upper slot of the condenser.
STAG E
The stage 8 (Fig. 2) is fixed on the microscope stand. There is an attachment on the stage that enables movement
of the specimen in two mutually perpendicular directions. The specimen is moved using the knobs 7 (Fig. 2) located
on the same axis. The control knob is low so the user will have no right-hand strain.
Stage size: 227mm×208mm. Moving range: 77mm longitudinal, 112mm lateral.
The stage design allows for using various laboratory glassware – flasks, well plates, dishes, cuvettes. The microscope
kit includes the following dish holders:
– 34mm×77.5mm; Ø68.5mm;
– 57mm×82mm; Ø60mm;
– 29mm×77mm; Ø90mm.
CAMERA
The digital camera equipped with the SONY CMOS sensor delivers high light sensitivity and low noise performance, and
it features an auto focus function. The camera operates autonomously, with no connection to the computer.
The camera is mounted into the trinocular tube using the C-mount adapter from the microscope kit.
The camera is powered via a 12V/1A AC power adapter.
MONITOR
The monitor is designed to use a visualization system of the MAGUS microscope. It is connected to the camera mounted
on the microscope to display the real-time images.
The monitor is installed on a folding mount on the table or shelf or attached directly to the camera or microscope
stand.
The monitor is powered by AC, DC 5-12V/1A (Type-C).
3 UNPACKING AND ASSEMBLING THE MICROSCOPE
The assembly procedure is given in Fig. 3.
1. Remove the microscope from the package.
2. Check the scope of delivery in item 8 of the User Manual.
3. Inspect the microscope and its components for damage.
4. Take out the microscope head 3, remove the dust cover. Insert the microscope head into the holder 2. Secure
the microscope head with the screw 9 using an Allen wrench from the microscope kit.
5. Remove dust caps from the eyepiece tubes. Insert the eyepieces 5 into the eyepiece tubes 10. Rotate the
eyepieces, making sure they are tightly seated in the tubes. Secure the eyepieces with the screw 4.
6. Remove the phase-contrast slider 7 from the package and install it in the condenser slot.
7. Install the color filter 6 in the condenser slot.
12
10
6
7
8
5
4
3
9
2
1
Fig. 3. Assembling the microscope
8. Remove the desired dish holder 8 from the package and place it in the stage attachment for moving the specimen
in X/Y directions. If you do not need to move the specimen in X/Y directions, loosen the screws and remove
the attachment.
9. Connect the AC power cord to the connector on the back panel of the stand. Plug the power cord into an AC outlet.
10. Make sure that all the components are securely and safely mounted.
11. Check and sort the supplied accessories and tools in the correct order. Keep them in proper order to avoid confusion.
4 BRIGHTFIELD OBSERVATION PROCEDURE
SWITCHING ON THE ILLUMINATION
Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the
local mains voltage. If not, do not switch on the microscope. Improper input voltage may result in a short circuit or fire.
Make sure that the power cord is plugged into the connector on the back panel of the microscope stand.
Turn the ON/OFF switch 1 to "–" position.
Adjust the brightness using the ring 2 so that the light brightness is 70% of full power.
Do not keep the brightness adjustment ring in the maximum brightness position for a long period. This may shorten
the life of the bulb. Before switching off the microscope, reduce the light intensity to the minimum.
1
2
Fig. 4. Switching on the illumination and adjusting the brightness
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ADJUSTING THE CONDENSER
Rotate the phase-contrast slider 1 to the center position corresponding
to the transmitted light.
1
1
Fig. 5. Adjusting the condenser
ADJUSTING THE EYEPIECE TUBES
Adjust the interpupillary distance. Adjust the distance between
the eyepieces to your interpupillary distance
by rotating the eyepiece tubes 1 (Fig. 6) around the central axis
until you see a single circular image when looking through
the eyepieces with both eyes (Fig. 6 a, b).
We recommend memorizing your interpupillary distance
for future reference.
1
b
a
Fig. 6. Adjusting the interpupillary distance
PLACING THE SPECIMEN
a
1
2
3
14
Fig. 7. Choosing and installing the dish holder
b
Y
X
1
2
3
Fig. 8. Moving the specimen
The stage attachment features an XY control system. The control knobs are coaxial – they are located on the same axis.
The knob 2 controls longitudinal (Y-axis) movement, the knob 3 controls lateral (X-axis) movement. Moving range:
77mm in Y-axis direction and 112mm in X-axis direction.
The stage attachment is driven by a rack and pinion gear. Once it has reached the limit, do not rotate the knob 2 or 3
as this may cause the gear to break. Be careful not to hurt your hand on the protruding rack.
FOCUSING ON THE SPECIMEN
Focusing on the specimen is achieved by coarse and fine focusing knobs.
Perform the focusing using the 10x objective.
Rotate the revolving nosepiece 1 to place the 10x objective in the optical path. The revolving nosepiece is rotated
until locked.
Rotate the coarse focusing knob 3 or 6 to raise the objective all the way up. Looking into the eyepiece and slowly
rotating the focusing knob, lower the objective. When you see the specimen image in the field of view, stop rotating the
coarse focusing knob.
Rotate the fine focusing knob 2 to focus on the specimen and get a crisp image.
Engage the coarse focusing lock knob 5.
3
4
5
6
2
1
Fig. 9. Focusing on the specimen
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When using high magnification objectives, raise the objective all the way up by rotating the coarse focusing knob and
enable the coarse focusing lock knob. After that, focus on the specimen using the fine focusing knob.
Adjust the coarse focusing tension.
The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use. If you
need to adjust the tension of the coarse focusing knob, rotate the coarse focusing tension adjusting knob 4. By rotating
it counter-clockwise you tighten the tension, and by rotating it clockwise you loosen it.
Too high a tension can affect the microscope performance and cause inconvenience in the operation.
ADJUSTING THE DIOPTER
One of the eyepieces has a diopter adjustment 1 to compensate for the difference
in vision between the user’s eyes. The adjustment range is ± 5 diopters.
Place the 40х objective into the optical path. While looking through the eyepiece
without diopter adjustment (with the other eye closed), bring the specimen into
focus. While looking through the eyepiece with diopter adjustment (with the other
eye closed) and not touching the focusing knobs, bring the specimen into sharp
focus by rotating the diopter adjustment ring.
1
Fig. 10. Adjusting the diopter
O BSERVING SPECIMENS IN TALL GLASSWARE
To observe specimens in tall glassware, remove the mechanical XY stage attachment, and then remove the dish holder 3.
To do this, use the screwdriver to loosen three attachment screws 1 below the stage and remove the XY attachment 2. Put
the attachment aside – lay it flat on a surface with the handle facing up so that it will not fall or be damaged.
2
1
3
16
Fig. 11. Preparing specimens for observations in tall glassware
CALCULATING THE TOTAL MAGNIFICATION
The total magnification is the eyepiece power multiplied by the objective power.
For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the total magnification of the microscope is
10х40 = 400х.
CALCULATING THE FIELD OF VIEW
The field of view is calculated by dividing the eyepiece field number by the objective magnification.
For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the field of view of the microscope is
22mm/40х = 0.55mm.
A stage micrometer (calibration slide) is used to accurately determine the field of view of the microscope.
USING THE CAMERA
The digital camera is equipped with a 2MP sensor producing a realistic image in Full HD resolution (1920x1080 pixels).
The microscope is designed to observe a specimen through the eyepieces and to photograph the specimen.
The trinocular tube is located on the microscope head and covered with a dust cap 2.
1
2
1. Attachment screw
2. Dust cap
3. Camera port centering screw
4. Camera
3
4
Fig. 12. Using the camera
To enable the camera:
– Loosen the attachment screw 1 of the trinocular tube, remove the dust cap.
– The microscope kit includes a C-mount adapter. Connect the camera 4 to the adapter.
– Fit the camera 4 into the trinocular tube and secure it with the screw 1.
– Place the 10х objective into the optical path. Looking through the eyepieces, bring the specimen into sharp focus.
– Switch on the camera according to the manual, adjust the image.
– If the image on the screen is blurred, adjust the focus using the fine focusing knob to ensure a clear and crisp image.
If there is a strict requirement to synchronize the image in the eyepieces and camera (coincidence between the image
center and direction), you should adjust the camera image using three centering screws 3.
17
Do it as follows:
– While observing the specimen through the
eyepieces, find a distinctive point in the field of view
(an easily identifiable target, such as point S
in Fig. 13а), move the specimen on the stage so that
the point is in the center of the field of view,
as shown in Fig. 13b. To do this, you should use
a special calibration slide with a reticle instead
of a specimen slide and an eyepiece with a reticle
in place of an ordinary one.
a
b
Fig. 13. Adjusting the camera image
– Look at the specimen on a monitor or display screen and make sure that the image of the target is in the center
of the field of view. If the image deviates from the center of the field of view, adjust three centering screws 3
on the trinocular tube to move the target towards the center.
– Move the specimen and check whether the image of the specimen on the monitor or display screen moves in
the same direction as the specimen does. If the image moves in another direction, you should adjust the camera
position. Loosen the lock screw 1, rotate the camera to make the displayed image direction in line with the direction
of stage movement, then secure the screw.
USING THE MONITOR
The IPS matrix delivers a bright image with large viewing angles, which allows you to look at the display even at
an angle with no color distortion.
To display the image on the screen:
– Attach the monitor 4 to the camera 1 using the fasteners from the kit.
– Connect the monitor to the camera using the HDMI cable 3.
– Connect the monitor and camera to AC power using the DC/DC Type-C adapter and power adapter (supplied).
If the camera and monitor are remote from each other, each device is powered separately using the power adapter
supplied with both the camera and monitor.
– Switch on and adjust the camera as per the user manual and the above steps in the previous section.
– Switch on the monitor by pressing the lower button on the side panel as indicated by the arrow
(not shown in Figure 14).
If the image on the screen is blurred, rotate the fine focusing knob to get an accurate image.
7. USB Type-С
power connector
8. Audio output
1. Camera
2. HDMI port
3. HDMI cable
4. Monitor
5. HDMI port
6. USB Type-С
power
connector
4
3
2
1
18
8
7
6
5
Fig. 14. Using the monitor
5 PHASE-CONTRAST TECHNIQUE
The condenser allows utilizing the phase-contrast technique. Brightfield observations are performed as described
above in the user manual. The following instructions apply only to the phase-contrast operation mode.
Adjusting the phase-contrast device:
1. Fully open the aperture diaphragm 5.
2. Take the 10х phase-contrast objective from the microscope kit and place it into the optical path. Place the
corresponding phase-contrast annulus of the phase-contrast slider 3 into the optical path.
1
2
3
b
c
d
6
4
a
5
7
8
Fig. 15. Phase-contrast technique
When using other phase-contrast objectives, place the corresponding phase-contrast annulus into the optical path.
3. Mount the specimen on the stage, focus the microscope.
4. Remove the eyepiece from the tube and insert the centering telescope 2 instead. You will see a dark ring 8 and
a light ring 7 in the field of view.
5. If the images of the ring edges are blurred, adjust the position of the movable part on the centering telescope 1,
focus on the rings.
6. In the phase-contrast observations, rings 7 and 8 must be coincident. If they do not coincide, use the screws 4
and 6 adjusted by an Allen wrench to center the diaphragm ring. The image shall be as shown in Fig. 15d.
7. Replace the centering telescope with a regular eyepiece.
When you change an objective, you should center the corresponding condenser phase annulus.
19
6 USING OPTIONAL EQUIPMENT
EYEPIECE WITH A SCALE
The eyepiece with a scale or reticle can be used to make comparative analysis of
the linear dimensions of the individual components of an object. The scale is
1 DIV. = 0 . 0 1 MM
installed in the plane of the field diaphragm of the 10x eyepiece. The eyepiece
with a scale is installed in the tube in place of the eyepiece of your microscope
You should use a special stage micrometer (calibration slide) to determine
the linear dimensions (in millimeters or microns).
The calibration slide is a transparent glass (of the same size as the specimen
Fig. 16. Calibration slide
slide) that has a micrometer scale with a scale division of 0.01mm etched on
the surface.
Place the calibration slide on the stage instead of the specimen with the scaled side facing up. Using the scale of the
calibration slide, calibrate the eyepiece scale for each objective that will be used for measurements. To do this, bring
the image focus of the calibration slide scale into sharp focus in the plane of the eyepiece scale and rotate the eyepiece
in the tube, setting the strokes of both scales in parallel. Determine how many divisions of the calibration slide fit in the
eyepiece scale (with the medium and high magnification objectives) or how many divisions of the eyepiece scale are
covered by the entire calibration slide (for low magnification objectives).
Work out the value for one eyepiece division using each objective by formula: Е = ТL/A, where
E – eyepiece division value
Т – stage division value specified on the stage micrometer (0.01mm)
L – number of stage micrometer divisions
A – number of eyepiece divisions.
We recommend entering the obtained data in a size chart:
Objective magnification
Eyepiece division value
10
20
40
Using these data to determine the actual linear size of the specimen, you just need to count the number of divisions
of the eyepiece scale aligned with the area of the specimen being measured, and multiply this number by the scale
division value specified in this table.
USING A CALIBRATION SLIDE WITH A CAMERA
The calibration slide (stage micrometer) is used to calibrate the image analysis software for measurements in actual
units. In the calibration mode, you should capture an image of the micrometer scale with every objective magnification
and indicate the known distance. That lets you establish a scale of the image in actual units (micrometer, millimeter, etc.).
Calibration:
1. Place the calibration slide on the microscope stage.
2. Select the desired objective and set the maximum camera resolution.
3. Get a contrast image of the scale on the monitor screen and capture the image.
4. Select the 'Calibrate' function in the software you are using.
5. Double-click on the maximum visible distance and enter the value in actual units.
6. Enter the calibration setting and check the result. The program will save the calibration factor.
7. You can select any measurement unit later, and all the results will be re-calculated in accordance with this selection.
20
7 TROUBLESHOOTING
Potential problems and remedies are given in Table 3:
Problem
Cause
Remedy
E L ECTRI CAL C OM P ONENTS
No illumination in the field
of view
The ON/OFF switch is off
Switch on the ON/OFF switch
The fuse has blown
Replace the fuse
Poor electrical contact
Check all the connectors. Have it repaired
by a qualified electronics technician
The installed bulb does not comply
with the specifications
Use the appropriate bulb
OPT IC S AND I M AGE REP RODUCTI ON
The revolving nosepiece is not clicked Rotate the revolving nosepiece into the
in the observation position (the
fixed position, i.e. position the objective into
objective is not in the optical path)
the optical path
The condenser is incorrectly
Darkened edges of the view field positioned – lowered too far or skewed
and uneven illumination of the
The diaphragm is not properly
field of view
centered or closed too much for this
objective
There is dirt or oil on the objective,
eyepiece, or condenser surfaces
Dust is visible in the field of view There is dust on the eyepiece lens
Poor image quality
(low resolution, poor contrast)
Adjust the condenser – set up Köhler
illumination
Center the diaphragm. Open the diaphragm
to illuminate the entire field of view
Remove dust using a special puffer or
brush. Clean the lens surfaces with a tissue
moistened with O-xylene
Remove dust using a special puffer or brush
The objective is damaged
Have the objective repaired by a qualified
technician or replaced
Inappropriate dish bottom thickness
Use the glassware with a standard bottom
thickness (1.2mm)
The aperture diaphragm is opened too Adjust the opening to match the numerical
wide
aperture of the objective used
The objective is not correctly engaged Rotate the revolving nosepiece until it clicks
in the optical path
into place correctly
The focal plane of the image is
tilted (brighter on one side and
darker on the other)
The specimen does not lie flat on the
stage
The phase contrast is not visible The wrong phase annulus was
selected
The phase-contrast annulus is not
centered
Place the specimen flat on the stage,
securing it with the specimen holder
Choose the proper phase annulus to match
the selected phase-contrast objective
Center the phase-contrast annulus
MEC H ANI CAL C OM P ONENTS
The image does not remain
sharp during observation
The coarse focusing tension adjusting Adjust the coarse focusing tension
knob is loosened, causing the stage to adjusting knob
lower spontaneously
The coarse focusing knob is too
tight to rotate
The coarse tension adjusting knob is
overtightened
Loosen the tension of the coarse
focusing knob
The specimen image when
viewed with two eyes in two
eyepieces does not coincide
The eyepiece tubes of the binocular
head are not adjusted to the
observer’s interpupillary distance
Adjust the microscope head
21
8 SCOPE OF DELIVERY
The scope of delivery is listed in Table 4:
Component
Pcs
Note
MICROSCOPE
MAIN COMPONENTS
Stand with built-in power supply, transmitted light source, focusing mechanism,
and condenser
1
Trinocular microscope head
1
Revolving nosepiece for 4 objectives
1
Mounted on the stand
Fixed stage
1
Mounted on the stand
Mechanical XY stage attachment
1
Mounted on the stand
REPLACEABLE PARTS
Phase-contrast slider with centerable phase annuli
1
Infinity plan achromatic objective: PL 4x/0.10 WD 21.0mm
Infinity plan achromatic objective: PL L PHP2 10х/0.25 phase-contrast WD
4.3mm
Infinity plan achromatic objective: PL L 10x/0.25 WD 4.3mm
Infinity plan achromatic objective: PL L PHP2 20х/0.40 phase-contrast WD
8.0mm
Infinity plan achromatic objective: PL L 20х/0.40 WD 8.0mm
Infinity plan achromatic objective: PL L PHP2 40x/0.60 phase-contrast WD
3.5mm
Infinity plan achromatic objective: PL L 40x/0.60 WD 3.5mm
1
10x/22mm eyepiece
2
10x/22mm eyepiece with a scale
1
Optional
15x/15mm eyepiece
2
Optional
20x/12mm eyepiece
2
Optional
25x/9mm eyepiece
2
Optional
Centering telescope
1
Set of dish holders
1
C-mount camera adapter
1
Calibration slide
1
Optional
Head locking screw
1
Installed in the stand socket
Set of Allen wrenches
1
Dust cap
2
Supplied
9W LED
1
In the lamp house
Color filter
3
Optional
One in the lamp house
Optional
1
1
1
Optional
1
1
Optional
1
ACCESSORIES AND SPARE PARTS
Fuse
2
AC Power cord
1
Dust cover
1
User manual
1
DIGITAL CAMERA
Digital camera
1
HDMI cable (1.5m)
1
USB mouse
1
32GB SD memory card
1
22
Wi-Fi USB-adapter
1
EU-plug 12V/1A AC power adapter
1
Flash drive with drivers and software
1
Fasteners (mounting plate and screws)
1
User manual
1
MONITOR
Monitor
1
HDMI cable
1
AC power adapter
1
DC/DC Type-C adapter
1
Fasteners (Allen wrench and screws)
1
9 CARE AND MAINTENANCE
REPLACING THE BULB
This microscope employs LED bulbs as a light source.
The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself,
the illumination function may be impaired.
REPLACING THE FUSE
Before replacing the fuse, turn the ON/OFF switch 1 to "0"
position (off). Unplug the power cord from the power outlet.
The fuse of the microscope’s lamp house is part of the electrical
circuitry used to power the bulb. The fuse is built into the inlet
power connector 3. It is replaced as follows:
1
– Using a flathead screwdriver 2, remove the fuse holder.
Remove the blown fuse and replace it with a new one.
Install the fuse holder back into the inlet power connector.
2
– Plug the power cord and turn on the ON/OFF switch
to check the fuse for proper operation.
Fig. 17. Replacing the fuse
3
MAINTENANCE
1. Once you have finished using the microscope, switch off the power supply. When not using the microscope for
a long time, switch off the power supply.
2. The microscope should be kept clean. Remove any residue of oil from the lenses, clean the microscope body with
gauze cloth (silk or cotton cloth) moistened with a small amount of alcohol. Do not install the dust cover unless
the microscope is completely cooled down and dry.
3. Cleaning lenses:
Remove dust from the lenses with a soft brush.
Significant contamination can be removed using a soft
cloth moistened with a small amount of a mixture of
alcohol and ethyl ether (mixture proportion: 20–30%
alcohol and 70–80% ethyl ether) or special O-xylene
solution. Wipe the lenses from the center outward.
Fig. 18. Cleaning lenses
Incorrect
Correct
23
4. Cleaning the surfaces: wipe with a clean soft cloth; significant contamination can be wiped off with a neutral
detergent.
Do not wipe the microscope stand with any organic solvent (e.g., alcohol, ethyl ether or its diluted solution). This
may cause damage to the coating of the microscope stand surface.
5. Cleaning the camera: blow off dust and small particles or brush them off with a soft brush, then clean the surface
with a soft, clean cloth moistened with alcohol or ether.
6. Monitor cleaning: brush the dust and small particles with a soft brush. If there are liquid drops on the screen,
remove them with a dry cloth or soft tissue. Use special alcohol wipes to remove heavy soiling.
Always switch off the monitor before cleaning. Do not use aggressive agents to clean difficult stains, as this may
cause damage to the device.
7. Storage: when not using the microscope for a long time, switch off the power, wait for the lamp to cool down, cover
the microscope with a dust cover, store the microscope in a dry, ventilated and clean place, with no exposure to
acids, alkalis, or steam, otherwise mold may form on the lenses.
It is recommended to apply a layer of rust-preventive coating to the moving parts of the microscope.
8. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance.
10 MAGUS WARRANTY
MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The
Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants
that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with
terms and conditions of transport, storage, and operation of the product. The warranty period for accessories is 6 (six)
months from the date of purchase.
For more information on warranty terms and conditions, see www.magusmicro.com.
For warranty service, please contact your nearest Levenhuk representative office.
24
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