Rotor-Gene® Q User Manual

Analysis User Interface

Run Archive...: This saves in a more compact file format.

Save files in this format before they are emailed. This reduces the time required to send the file and ensures that files are not corrupted by e-mail clients.

LIMS Export This saves the analysis in LIMS compatible formats according to the requirements of the user. Please contact QIAGEN Technical

Services for more information.

Excel Data

Sheet...:

Excel Analysis

Sheet...:

LinReg Export

Format...:

This exports all the raw channels to an

Excel ® sheet. Only the selected samples are exported.

This exports all the analysis in the current run into a single Excel sheet.

This exports all raw channel data into a format that can be read by LinReg (an efficiency analysis tool). See “Exporting To

LinReg” below for more details.

Matlab Export...: This exports the data into a format that can be read by the scientific package Matlab

(or its open-source equivalent, Octave).

This may be useful for methods research.

Exporting To LinReg

LinReg is a tool developed by C. Ramakers and coworkers.*

The LinReg tool is available from: http://LinRegPCR.nl.

Rotor-Gene Q software allows the user to export raw data in a format that can then be imported by the LinReg tool for analysis.

1. Open the Rotor-Gene Q run file containing the raw data.

*

Ramakers, C., Ruijter, J.M., Deprez, R.H., and Moorman, A.F. (2003)

Assumption-free analysis of quantitative real-time polymerase chain reaction

(PCR) data. Neurosci. Lett. 339, 62.

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7.5.3

Analysis User Interface

2. Export the data to the LinReg export format by selecting

“Save As…” and then “LinReg Export Format…”.

3. Microsoft Excel automatically displays the exported raw data.

4. Start up the LinReg tool.

5. The tool asks you to select the cell range where the raw data is located. The tool can only analyze one raw channel at a time, so an appropriate region of the Excel sheet should be selected.

Reports

After selecting “Reports”, the “Report Browser” window appears. If the data has already been analyzed, the report of that analysis can be displayed from the “Report Browser” window. Several report types are offered with varying degrees of detail.

7.5.4 Setup

The initial setup of the Rotor-Gene Q should be completed during installation. However, this option allows you to change the Rotor-Gene Q connection setup, if you wish to do so after installation.

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Analysis User Interface

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Virtual Mode: Select this option if the software will be used without a connected Rotor-Gene Q.

The software retains all functions. This mode is useful for demonstration purposes, data analysis, and setting up templates.

Allow access to this setup screen:

If this option is not checked during setup, this window can no longer be accessed.

This security measure prevents users from altering the settings. To reestablish access, contact your distributor.

Port: Select the correct communication port to enable communications between the computer and the Rotor-Gene Q. If you are unsure which port to select, click “Auto-

Detect” to search for all available ports.

Auto-Detect If you are unsure which port to select, click

“Auto-Detect” to search for all available ports.

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7.6

7.6.1

Analysis User Interface

Analysis menu

Analysis

After clicking “Analysis”, the “Analysis” window appears. This window allows creation of new analyses and display of existing analyses. The method of analysis is selected using the tabs. A list of the channels that can be analyzed using the selected method is shown. Multiple assays run in the same channel can be analyzed independently, provided they have been set up as separate pages in the “Edit Samples” window.

Pages that have already been analyzed have a green checkmark next to them. This means that threshold and normalization settings have been saved for this analysis. To view or analyze a channel, double-click on it. The specific analysis window appears.

Auto-shrink window:

Selecting “Auto-shrink window” shrinks the window when it is not in use. Moving the cursor over the window enlarges the window again.

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