FL WinLab User`s Guide

TLC Scan

Application

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Introduction .................................................................................... 13-2

Menu commands............................................................................. 13-2

Toolbar ........................................................................................... 13-3

Using the application...................................................................... 13-4

Parameter Pages.............................................................................. 13-5

Setup Page ................................................................................. 13-5

Realtime Options Page .............................................................. 13-9

User Info Page ......................................................................... 13-10

View Results Page................................................................... 13-12

TLC Scan

Application

13

The TLC Scan application enables luminescence measurements to be made at fixed wavelengths for an individual position, a single scan or a multiple scan over the surface of any flat sample, TLC plate or gel.

TLC Scan Application

Introduction

The TLC Scan application enables luminescence measurements to be made at fixed wavelengths for an individual position, a single scan or a multiple scan over the surface of any flat sample, TLC plate or gel.

Multiple scans are performed with a selected data collection interval, resulting in a 3D plot which can be viewed in the 3D View application.

Menu commands

File Menu

Commands for opening, saving and printing methods and exiting the application. For details see chapter 3, Working with Application

Methods.

Instrument Menu

Contains commands for starting and stopping data collection.

Help Menu

Contains commands for using the online help.

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TLC Scan Application

Toolbar

The TLCScan application displays a graphic toolbar for real-time graphic functions. The graphic icons are visible when the View

Results page is opened or when a run is started. The following generic

View buttons are described in Chapter 5:

Auto-expansion of X-axis

Auto-expansion of Y-axis

Format graph ranges

Select default Y-range

Radar Window

Vertical cursor

Remove curve

Printout of data

Copy to clipboard

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TLC Scan Application

Using the application

A general description of working with application methods is given in chapter 3.

1. In the FL WinLab window open the Application menu and click on TLC Scan. The TLC Scan application is opened.

2. Enter the parameters on each page of the application. To move from on page to the next, click on the tab on the top of the page.

3. Click on the green traffic light (Start / Sto

p

button) in the toolbar to start the method.

4. Open the page Viewing Results to look at your data. You can use the icon in the toolbar to format the graphic display.

5. To exit the application, open the File menu of the application and click on Exit.

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Parameter Pages

Setup Page

TLC Scan Application

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Excitation wavelength

Click on the textbox and enter the excitation wavelength in nm.

Emission wavelength

Click on the textbox and enter the emission wavelength in nm.

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TLC Scan Application

Excitation slit

The excitation slit width is the spectral band width of the excitation monochromator. In order to obtain the best spectral resolution, select a narrow slit width, e.g. 2.5 or 5 nm. The best signal-to-noise ratio is obtained by selecting a large slit width.

Emission slit

The emission slit width is the spectral band width of the emission monochromator. In order to obtain the best spectral resolution, select a narrow slit width, e.g. 2.5 or 5 nm. The best signal-to-noise ratio is obtained by selecting a large slit width.

Data interval

Click on the textbox and enter the required data interval (interval between two measuring points) in millimetres.

Plate scan speed

The plate scan speed controls the time for the fibre optic to scan across the plate/gel.

Plate image

This page displays an image of the maximal scannable area of the plate. Click with the left mouse button and drag to draw the scan trace(s). The left, right, top and bottom textboxes are updated accordingly. Click with the right mouse button to move the probe head and measure the intensity at that position. If the old plate reader accessory is fitted the limited x and y scan range is indicated by a red frame.

Scan in X-direction button

Click on this button to scan the plate from left to right in the Plate

Reader Accessory.

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TLC Scan Application

Scan in Y-direction button

Click on this button to scan the plate from back to front in the Plate

Reader Accessory

Immediate start

Select this option if you wish to start data acquisition immediately after clicking on the green traffic light subsequent to setting up the instrument without seeing the OK to start panel.

Single scan button

Click on this button to scan a single trace of the plate/gel.

Multiple scans button

Click on this button to scan multiple traces and create a 3D plot of the plate/gel.

Current X and Y Position

The current X and Y position of the cursor over the plate image (or the intensity at the current probe head position) is displayed on this box. The textbox below the current position shows the last measured intensity (measure the intensity by right clicking on the desired position on the plate image). This function is also useful in determining the positional parameters for a 3D scan.

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TLC Scan Application

Left, right, top, bottom and delta textboxes

To align the plate, specify the distance from the edges of the plate to the scan start and stop points. In the Left textbox enter the distance from the left edge of the plate to the scan start point. In the Right textbox enter the distance from the left edge of the plate to the scan end point. In the Top textbox enter the distance from the top edge of the plate to the scan start point. In the Bottom textbox enter the distance from the top edge of the plate to the scan end point. In the

Delta textbox enter the distance between successive traces during a multiple run.

Redraw button

Click on this button to redraw the plate scan lines to reflect the values in the textboxes Left, Right, Top, Bottom, and Delta.

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TLC Scan Application

Realtime Options Page

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Ordinate label

Enter the desired ordinate label in this textbox. The label is stored in the dataset. It is displayed on the ordinate axis of the graph.

Ordinate Max and Min

The ordinate maximum and minimum values are used as defaults for the ordinate range displayed in the graph when a measurement is started. If the textboxes are empty the graphs ordinate range does not change when a measurement is started. Furthermore the „Select default Y-range“ button

Auto-clear curves

If this option is selected all curves are deleted from the graphic before a measurement starts. The curves are not deleted from the harddisk.

Immediate start

Select this option to start the data acquisition immediately after clicking on the green traffic light subsequent to setting up the instument without seeing the OK to start panel.

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TLC Scan Application

User Info Page

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Analyst

This textbox contains the name of the current analyst. The name is automatically taken from the benchtop´s configuration dialog, but can be altered for documentation purposes. The name is saved in the header of any collected datasets and, if a method is saved, in the header of the method.

Sample info

The information entered in this textbox is stored in the dataset header.

The first line of the information is stored in the "comment“ field of the header, which is displayed in all graph windows. The complete information is stored in the "FL memo“ field of the dataset. This can be obtained using the report builder.

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TLC Scan Application

last modified / by

This shows the last date of saving the current method and the name of the analyst who saved the method.

locked

This option allows the locking of all entries in a method. The option can be altered in Expert mode only. If the option is selected all entry fields and the menu topic "method save“ are disabled.

Comments

The comment in this textbox is saved in the method header. It is also displayed in the method window of the benchtop. This comment is

NOT saved in any dataset. Use the sample info textbox for comments to be saved in datasets.

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TLC Scan Application

View Results Page

This page contains an extra toolbar, in which graphics icons appear.

For further details on viewing data see chapter 5.

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