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OM0211
Air-Jacketed DH Invitrocell CO
2
Incubator
Models
NU-5800 (Series 1)
NU-5800E (Series 1)
Operation and Maintenance Manual
March 2013
Revision 3
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
Page 1 of 48
Air-Jacketed DH Invitrocell CO
2
Incubator
Operation & Maintenance Manual
NU-5800/NU-5800E
Table of Contents
Section No. 1 ..................................................General Description
Section No. 2 ..................................................Performance Parameters and Features
Section No. 3 ..................................................Models & Features
Section No. 4 ..................................................Test Performance & Procedures
Section No. 5 ..................................................Warranty
Section No. 6 ..................................................Shipments
Section No. 7 ..................................................Installation
Section No. 8 ..................................................DH Operation
8.1 ..............................................Sterility
8.2 ..............................................Humidity
8.3 ..............................................Control System Introduction
8.4 ..............................................Front Control Panel
8.5 ..............................................Rear Component Panel
8.6 ..............................................Run Mode Operator Interactions
8.7 ..............................................Setup Mode Operator Interactions
8.8 ..............................................Diagnostic Interactions
Section No. 9 ..................................................Calibration
9.1 ..............................................Chamber Temperature Calibration
9.2 ..............................................Door and Perimeter Heater Adjustments Affecting Temperature Calibration
9.3 ..............................................CO
2
Calibration
Section No. 10 ................................................Maintaining Your DH Invitrocell
10.1 ............................................Chemical Decontamination
10.2 ............................................Shutting Down the Incubator
10.3 ............................................In Filter Change Notification
Section No. 11 ................................................Error Indicators & Troubleshooting
Section No. 12 ................................................Communication Outputs
Section No. 13 ................................................Electrical/Environmental Requirements
Insert ..............................................................Replacement Parts List
MANUAL DRAWINGS
BCD-14784 .................................................... Dimensioned Assembly NU- 5800/E
BCD-15169 .................................................... Shelf/water pan Installation
BCD-15669 .................................................... Rear Component Panel Assembly
ASSEMBLY DRAWINGS
BCD-15180 .................................................... Front Component Panel
BCD-15177 .................................................... Electronics Panel Assembly
BCD-15634 .................................................... Sensor Bay Assembly
BCD-15178 .................................................... NU-5510/E Tubing Configuration
BCD-15191 .................................................... Display / Outer Door Assembly
ELECTRICAL SCHEMATICS
BCD-15670 .................................................... Electrical Schematic, NU-5800/E
BCD-15190 .................................................... Main Control Board Electrical Schematic
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DH Direct Heat
Automatic CO
2
Incubator
Models: NU-5800 and NU-5800E
Operation and Maintenance Manual
1.0 General Description
The NuAire Direct Heat (DH) Automatic CO
2
Direct Heat Incubator has been designed to provide a reliable controlled in-vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage and preservation of embryos, gametes and animal tissue cell cultures intended for research at or near body temperature. There are five parameters that contribute to optimum growth conditions.
These are:
Humidity
Precise temperature control
Precise CO
2
Sterility
Reliability
control
Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with matching computer technology, precise temperature control, and CO
2
gas control system combining state-of-the-art technology with years of design, quality, and manufacturing experience.
1.1 Incubator Chamber
The design and size of the DH inner chamber provides a large capacity (7 cu. ft.), and ease of use. The chamber walls are directly heated by physically attached foil heating elements on the sides, bottom, top, and back of the chamber, providing a temperature uniformity of +0.3°C. A space-age high-density insulation that has a high "R" rating covers the complete outer surfaces of the Incubator inner chamber.
1.2 Incubator Closed loop chamber sample circulation system & HEPA Filter
A continuous operating air pump draws environmental sample from the chamber and circulates it through a HEPA filter capsule then through sensors that measure CO2 volume (as a percentage) in the chamber for monitoring and control.
1.3 Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer based control system specifically designed to service the precise control requirements of the chambers environment, providing optimum programmable conditions for culture growth. The microcomputer is “user-friendly” and provides a 5x7 LCD screen which provides operating control parameters, status indicators, additional key operational parameters, and an imbedded touch panel to permit efficient operator entry of data.
The microcomputer is supported with Read Only Memory (ROM) containing executable software, Random Access Memory
(RAM) for temporary storage, and Electronically Erasable Programmable Read Only Memory (EEPROM) for control set points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power interruption (power fault tolerant).
1.4 Incubator CO
2
Control
The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO
The amount of energy received at the detector is an approximate logarithmic function of the CO between source and detector. The wavelengths used are absorbed only by CO
2
2
induction sensor.
concentration in the gas
2
making the measurement insensitive to other components, such as water vapor. Detector linearization is performed with 32 bit digital accuracy. Advanced design provides a very stable output minimizing drift and requiring less frequent calibration. The output is digital, alleviating errors brought about by analog signals. Calibration of this control is accomplished by a zero & span calibration done on the sensor and a display off- set calibration. These calibrations are accessible through the NU-TOUCH LCD located on the front of the unit.
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1.5 Incubator Construction
The outer shell of the air-jacketed Incubator is cold-rolled steel with a powder coat paint finish. The front frame surface of the outer shell is heated with a foil type heater directly attached behind the front perimeter opening.
The front frame perimeter heater, as well as the outer front door heater is duty cycle controlled (manually adjusted for specific ambient conditions) to balance the heat that reaches the chamber and thus reduces the possibility of condensation forming on the inner glass door and the inner chamber walls.
The inner chamber is 16 gauge, type 304L, polished stainless steel using crevice-free construction, which provides an easily cleanable inert surface that does not in itself promote biological growth. In addition, all shelves, shelf supports, and guide rails are easily removable and can be autoclaved separately if so desired.
Remember: The chamber environment is not selective. The growth environment is applied equally to all microorganisms
(specimens and contaminates) within the chamber.
1.6 Incubator Humidity
A relative humidity level of up to 90% is achieved in the Incubator by the use of a stainless steel pan filled with distilled water no purer than 1 mega ohm, and placed on the bottom of the chamber. It is necessary to set the duty cycle of the door and front perimeter heater in proper proportions to reduce the possibility of condensation forming on the glass inner door and the chamber walls. It is also important to thoroughly wipe the walls and the glass door clean before adding the humidity water pan. Condensation will occur more readily at contamination points. There is no electronic sensing and thus no automatic control of the humidity level in the air jacketed Incubator. An air pump injects fresh air into the chamber at a preset duty cycle to reduce condensation forming on the chamber walls and front glass door. The air injections are settable by the user to help control condensation. (See section 8.8 for this option.)
1.7 Cabinet Ventilator Fan
An axial fan is mounted to the bottom cover panel of the Incubator and runs continuously when the unit is switched on.
This fan pulls air into the cabinet shell thru the top panel louver openings and discharges it out of the bottom of the unit.
The operation of this fan is necessary to assure accurate chamber temperature control over the 60°F to 85°F
(15°C to 30°C) ambient temperature range that the equipment may see.
1.8 Safety Instructions
These safety instructions describe the safety features of the INVITROCELL Incubator.
The incubator has been manufactured using the latest technological developments and has been thoroughly tested before delivery. It may, however, present potential hazards if it is not used according to the intended purpose or outside of operating parameters. Therefore, the following procedures must always be observed:
• The incubator must be operated only by trained and authorized personnel.
• For any operation of this unit, the operator must prepare clear and concise written instructions for operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in particular. o
which decontamination measures are to be applied for the incubator and accessories, o
which protective measures apply while specific agents are used, o
which measures are to be taken in the case of an accident.
• Repairs to the device must be carried out only by trained and authorized expert personnel.
• Keep these operating instructions close to the unit so that safety instructions and important information are always accessible.
• Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.
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1.9 Explanation of symbols
Symbol
!
!
WARNING
CAUTION
Description
Safety alert symbol indicates a potentially hazardous situation which, if not avoided, could result in death of serious injury.
Safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury.
Note:
Biohazard
Hazardous Gases!
Personal Protection Equipment Required.
Chemical Hazard
Ground, Earth
!
Attention accompanies information or important symbol
Potential electrical hazard, only qualified person to access.
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CAUTION
Flammable Hazard
Lead Free
CAUTION used without the safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in property damage.
Hot Surface
Burn Potential
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2.0 Performance Parameters and Features
• Both the interior and exterior of the DH are constructed of 18 to 16-gauge steel.
The interior is highly polished type 304 L 16 gage stainless steel, using crevice-free construction.
All exposed edges are deburred to insure no sharp edges.
The exterior is cold rolled 16 & 18 gauge steel finished in a powder coated polyurethane finish, which is resistant to chemicals and easily cleaned using mild household detergents.
• The DH's microcomputer temperature control system has two temperature sensors located inside the chamber at the back wall.
The temperature sensors compare the values to a set point and execute a differential control algorithm that energizes a solid-state switch, supplying power to the heaters.
• Easily removable inner shelves and rails for sterilization.
• Up to 21 shelves can be placed inside the chamber (4 shelves are shipped with each DH).
• Space-age high temperature material is used to insulate the inner chamber walls.
• Foil heaters are directly attached to the chamber walls and the top and bottom surfaces of the inner chamber.
• Most electronics, motors, pumps, and valves are fully accessible from the back of the unit.
• A CO
2
sample port is provided on the front panel just below the door to check the concentration of CO chamber.
2
in the
• The CO
2
percentage is controlled by a solid-state gas infrared sensor using a single light source with dual wave length detection, providing accurate monitoring of CO
2
.
• Automatic recovery of the CO
2
level, after a 1minute door opening, to 5.0 + 0.5% is within a 5 minute period.
• The outer door includes an internal radiant heater in order to minimize condensation on the inner glass door.
A magnetic outer door gasket helps to insure a tight seal against the cabinet.
• The inner glass door is 3/16” tempered with smooth-ground edges and seals are tight against a silicone rubber gasket.
The door latch is cam action.
A momentary switch monitors the door position, the position being opened or closed.
• All control electronics are protected with a circuit breaker that may trip at 110% of loading rating but will trip at
145% of load rating in less than 2 seconds.
Should the circuit breaker open (pop-out button will appear), merely depress to reset.
• The Incubator has factory-installed adjustable leveling legs to compensate for uneven laboratory surfaces.
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3.0 Models & Features
3.1 Dimensions (see also Specification Drawing BCD-14784)
Overall Dimensions - inches (mm): Model NU-5800/E
Height:
Width:
Depth:
Foot print:
Height:
Width:
Depth:
Shelf Capacity:
Size:
Supplied:
Max. Capacity:
Water Pan:
Dimensions:
Exterior: 39.688 in (1008 mm)
26.500 in (673 mm)
27.188 in (691 mm)
23.5 in (597 mm) wide x 20.75 in (527 mm) deep
Interior: 28.500 in (723.900mm)
Max. Weight Capacity
21.257 in (539.928mm)
20.053 in (509.346mm)
19.75 Inches (502mm) x 18.75 Inches (476.5mm)
4 Shelves
23 Shelves
20 lbs. per DH shelf
50 lbs. per DH Incubator
(Make sure shelf is empty before changing position or removing.)
12.0” (305mm)
10.0” (254mm)
2.5” (63mm)
Capacity:
3.2 Performance Parameters :
• Temperature Range:
• Temperature Uniformity:
• Temperature Accuracy:
• CO
• CO
2
2
Range:
• CO
2
Accuracy:
Recovery:
• Temperature Recovery:
• Temperature Display Resolution:
•
•
CO
2
Display Resolution:
Door and Perimeter:
• Temperature Sensor Type:
• CO
2
• CO
2
Control Logic:
Sensor Type:
Mean Length
Mean Width
Depth
Maximum
Recommended Fill
3.5 Liters
3.0 Liters
5°C to 55°C (5°C above ambient to 30°C max. ambient)
+0.35°C @ 37°C.
+0.1°C.
0.1 to 20%. (0.0 set point idles system)
+0.1%
Up to 5% +0.50% in 5 minutes average.
0.12°C/minute Average
0.1°C
0.1%
Proportional base duty cycle based on Temperature set point and 0-100% manually adjustable to adapt to ambient conditions.
Precision Integrated Circuit
Fixed Algorithm/Manual Environmental Adaptable.
Infrared single source dual wave length
• Connectors on rear panel are provided for Dry contact remote alarm, RS-485 2way communication, and 4 – 20 mA analog output signals for system performance.
Note: Performance ratings apply ONLY when the Incubator is set up properly for the installation.
See sections 8 and 9 for proper set up.
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3.3 Standard Items Packed With Unit
• Four (4) stainless steel shelves
• Eight (8) stainless steel shelf brackets
• One (1) water pan
• Gas tube
• Access port plug with breather hole
• One (1), 2 meter (6.5 ft.) electrical cord
• Operation and Maintenance Manual
• Operating Instructions
3.4 Consult Factory for additional Accessories (Ordered Separately)
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4.0 Test Performance & Procedures
All equipment is thoroughly inspected at the NuAire Factory at the time of shipment. Quality control is maintained by constant surveillance over the product, beginning at the receipt of purchased material and concluding with a final inspection, which certifies the Incubator performance to the specifications. In all instances where product quality cannot be easily assessed on the end item, the product is inspected during sub-assembly fabrication. The following test procedures are conducted on every DH Incubator and a copy of the test report is included with each Incubator.
4.1 Visual Inspection
• Each Incubator is visually inspected to insure that the interior is clean and free from scratches, nicks, and burrs and that all welds, both interior and exterior are finished.
• Painted surfaces are inspected to be free of scratches, nicks, insufficient covering, and noticeable paint runs.
• The doors open and close freely without binding of the hinges.
• The gasket seals the inner glass door to the Incubator tightly across its entire opening.
• The glass door is free of scratches.
4.1 Electrical Tests
• Electrical Leakage Test
All Incubators are required to have a primary-circuit filtering to meet EMC (electromagnetic compatibility) regulations.
Electrical leakage does not exceed 1.0 milliamperes.
• Dielectric Voltage – Withstand Test
1200 VDC (115 volt units) or 1970 VDC (230 volt units) is applied between dead metal parts and the hot/neutral power source lead with no electrical breakdown using an Associated Research Model 7564SA Quadchek II, 8106 Omnia 6, or
8006 Omnia 6, or other models.
• Grounding Continuity
The resistance between the green bonding conductor of the supply cord and any dead metal part of the cabinet shall not exceed 0.10 ohms.
4.2 Functional Tests
These functional tests are performed on every Incubator over a 48-hour final acceptance test/burn-in period.
All failures are noted, corrected, and the failed test is successfully repeated.
• Control Systems
All performance and diagnostic functions are exercised to insure proper operation of control systems, components, and alarms.
• CO2 Control
Each Incubator is calibrated to function at a 5% CO2 level.
The concentration is checked with an independent calibrated instrument.
Each unit is monitored during the 48-hour burn-in period and only accepted with zero failures.
• CO2 Recovery
Each Incubator is exercised for CO2 recovery time at the end of the 48-hour burn in period.
The door is opened for 1 minute to deplete the CO2.
After the door is closed, the unit shall recover to 5% +0.2 / - 0.5% within a 5 minute period.
• Temperature
Each Incubator is monitored for stable temperature control over the 48-hour burn-in period at (37°C).
• Humidity
Each unit has a default RH level intended to avoid condensation.
Door and perimeter duty cycles are determined by a Base duty cycle calculation based on the chamber
Temperature set point. These are the set at the factory but can be adjusted if needed to control condensation and tune in the chamber temperature uniformity for the environment that the incubator is installed in.
If unit is master reset these settings will be changed to a default value of 100.
The factory default door and perimeter settings are: Door: 100, Perimeter: 100.
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5.0 Warranty
NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory a similar part to replace any material in its equipment within 24 months after the date of sale if proven to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer and this warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this product is altered, removed, or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any product shipped to NuAire without this number will be returned, refused shipment or collect freight.
6.0 Shipments
NuAire, Inc. takes every reasonable precaution to assure that your Incubator arrives without damage. Motor carriers are carefully selected and shipping cartons have been specifically designed to insure your purchase arrives safely and undamaged.
However, damage can occur in any shipment and the following paragraph outlines those steps you should take on receipt of a
NuAire Incubator to be sure that if damage has occurred, the proper claims and actions are taken immediately.
6.1 Damaged Shipments
6.1.1 Terms are F.O.B. factory, unless stated otherwise.
Therefore, it is important to check each shipment before acceptance.
6.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your filing the claim against the carrier.
6.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the request. This, along with other papers in the customer's possession will support the claim.
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7.0 Installation
The Incubator is fastened to the base skid and it is usually the best procedure to leave the skid attached until the Incubator is located in its approximate position to facilitate ease in handling. The base skid can then be removed by removing the four bolts holding the cabinet to the skid. Then screw the leveling feet in to the same nuts that were used to fasten the incubator to the skid. The incubator must be a minimum of 2.0 inches above the floor. Use the 1.0” spacers provided on the threaded stem of each leveling foot when they are installed to ensure this spacing. See BCD-14784.
Examine the Incubator carefully.
INSPECT both the exterior and the interior for any transit damage before discarding the shipping crate.
7.1 Location
In locating the Incubator, consider all possible conditions that might affect its performance as well as laboratory procedures for its intended purpose. Do not locate near heating or cooling ducts, or next to equipment that generates heat (steam radiators, stoves, ovens, autoclaves, etc.). Avoid direct sunrays and rapidly moving air currents. These conditions adversely affect the even heat dissipation required from the exterior surfaces of the Incubator and may cause the temperature variation in the chamber to exceed specifications as stated in section 3.2. Since the Incubator needs even heat dissipation on all surfaces in order to maintain an internal temperature variation of less than ± 0.2°C, a minimum of 2 inches (50mm) must be allowed between the rear and sides of the Incubator and any walls, partitions or obstructions to facilitate adequate convection of air around the Incubator. Confirm clearance with a tape measure if needed. Adjust the Incubator location accordingly. In addition, the Incubator location should address access to its power cord for connection/disconnection if necessary. For maintenance and service purposes, the control center electronics should remain accessible. Leave at least 3 ft of clearance in front for the swinging door.
7.2 Leveling
Prior to use the incubator should be leveled using a bubble level on a middle shelf in the chamber. The Incubator should have all 4 leveling feet firmly on the bench or floor. Level from side to side and front to back. By turning the adjustable leveling feet counter-clockwise, raises the Incubator. The leveling feet height should allow for a 2 inches (50mm) space between Incubator base and the floor. There is a 1 inch long Stainless Steel spacer supplied with each foot. Be sure that it is in place when you are leveling the incubator.
Note: It is important that the bottom of the incubator be off the floor by AT LEAST 2 inches (50 mm) to assure cabinet air flow relief.
Spacers are provided on the threaded stems of the feet to hold this distance.
Confirm with a tape measure if needed.
7.3 Shelf & Water Pan Installation
Before installation of the shelves, and water pan, NuAire recommends to decontaminate all surfaces within the interior chamber, glass door, and outer door with gasket. They can be wiped down with a disinfectant of 70 percent alcohol or similar non-corrosive antimicrobial agent. Use only disinfectants that are compatible with the vinyl gasket, the powder coat paint on the outer door and the 304L stainless steel in the chamber. See BCD-15169
Note: Absolutely no chlorinated or halogen materials are to be used in the chamber.
Four (4) shelves are provided with each Incubator.
The shelves are easily installed by attaching the shelf brackets to the shelf supports.
If the shelf does not bind or disengage from the horizontal bracket it is installed correctly.
Additional shelves and shelf guides are available.
The water pan shall be placed directly on the bottom of the chamber.
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7.4 Electrical
The electrical supply circuit to the Incubator must conform to all national and local electrical codes.
Consult the serial-data plate, located at the front of the right side of the Incubator, for voltage, cycle, phase, and ampere requirements before making connection. Plug the power cord securely into a grounded power source. VOLTAGE SHOULD
NOT VARY MORE THAN 5% FROM SERIAL PLATE RATINGS. Have a qualified technician check with the power source with a properly rated volt meter if needed. A separate branch circuit is recommended to prevent possible loss of product due to overloading or failure of other equipment on the same circuit. A SURGE PROTECTOR IS STRONGLY RECOMMENDED to avoid power-related faults.
7.5 Air Inlet Connection
The air inlet is installed at the Factory and consists of flexible hose attached to bulkhead fittings in the bottom of the front panel and can be found under the hinged Valance that is beneath the door. One 50mm polypropylene .3-micron HEPA filter is connected to the hoses. The filter is labeled “Air filter”. To open the valence the outer door must be open. Reach in under the front of the valence and pop it open by pulling it toward yourself.
See BCD-15180
CAUTION:
This is a free (open air atmospheric) air supply. DO NOT connect to a pressurized air source.
7.6 CO2 Connection
!
CAUTION
High concentrations of CO
2
gas can cause asphyxiation!
Install Incubator in a well-ventilated area.
This Incubator is designed to be operated with CO condition. Gases other than CO
2
2
gas only. Connecting a flammable or toxic gas can result in a hazardous
should not be connected to this equipment. CO
2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve. Check the gas cylinder for the proper identification labels.
Do not use CO which can damage the pressure regulator. Consult with your gas supplier to ensure that the CO
2 siphon tube.
2
gas cylinders equipped with siphon tubes. A siphon tube is used to extract liquid CO
2 from the cylinder
cylinder does not contain a
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7.6.1 CO
2
Tube Connection
Included with the Incubator are a tubing kit consisting of (1) six foot (2m) vinyl tube and (1) 50mm polypropylene
0.3 micron HEPA filter. The filter is an extra for your convenience. Hook one end the six foot tube to the fitting labeled CO2 (20 psi) on the back component panel of the incubator. Then hook the other end on the outlet of the
2 stage regulator located on the gas supply. You can locate the CO2 supply filter on bottom right of the front panel behind the hinged valance at the bottom of the outer door. The filter is labeled CO2 filter. To open the valance the outer door must be open. Reach in under the front of the valance and pop it open by pulling it toward yourself.
The Filter supplied in the shipping kit is an extra one that can be used when it is time to change the one on the unit.
Please see the Maintenance section for the proper period to change the filter.
7.6.2 CO
2
Supply
• CO
2
of medical grade is recommended.
• A two-stage pressure regulator, Linde # 19590, or equal, is recommended.
• DO NOT USE a single stage regulator.
It will not give a stable output at 20 psi and exposes the Incubator to the gas cylinder pressure.
7.6.3 CO
2
Regulators
The regulator’s high-pressure stage direct from the supply cylinder must have a range of from 0 to 2000 PSI or
0 to 140 BAR. This gauge indicates actual tank pressure. The low-pressure stage should have a range of 0 to 30 PSI or 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge will indicate the actual CO single stage CO
2
pressure regulators have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use
CO
2
in such small quantities that precise metering of CO
To connect the regulator:
2 pressure to the Incubator. Some
2
input pressure is important for maximum performance.
First: Open the CO
2
cylinder slightly, for an instant (this is termed “cracking the valve.”)
This will blow out dust or dirt that may have collected in the valve outlet.
BE SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt.
Second: Make sure the regulator pressure-adjusting screw is released by turning it counterclockwise until it turns freely.
Third: Attach the regulator to the cylinder valve and tighten the connection nut with a wrench.
BE SURE DISC SEAL IS IN PLACE BEFORE MAKING CONNECTION.
7.6.4 CO
2
Connection
Connect the CO
2
supply from the low-stage of the two-stage regulator, to the inlet fitting located on the Incubator back panel labeled “CO2 tank 1”. The filter should be inserted downstream of the low-stage regulator before the inlet fitting on the Incubator as shown in the figure. Secure each connection with the clamps supplied. Observe proper flow orientation of the filter (look for “in” or a green dot on the filter). The tubing is easily cut with a sharp knife.
7.6.5 CO
2
Supply Adjustment
With the regulator OFF (i.e. fully counterclockwise), open the cylinder valve slowly usually 1 to 2 turns is sufficient.
20 PSI or 1.4 BAR. The CO
Note: OSHA requires the CO
7.6.6 Checking the Connections
2 tanks to be physically restrained (i.e. via chained to wall) to prevent accidental damage to cylinder.
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The cylinder tank pressure should read 700 to 800 PSI or 48 to 55 BAR, more or less, depending on the temperature of the cylinder. Next, turn the regulator’s pressure adjusting screw clockwise, until the low-pressure gauge reads
!
CAUTION
The connections can be checked for leakage by brushing a small amount of soapy water on each one.
Observe to see if any bubbles are blown. If not the connection is secured properly.
If it is, the cause of the leaking needs to be determined, (i.e. loose clamp or damaged hose) and corrected.
2
NEVER STAND IN FRONT OR BEHIND THE REGULATOR WHEN
OPENING THE VALVE. ALWAYS STAND TO ONE SIDE.
connection is now complete.
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7.7 Water Pan
Place water pan in the center on the bottom of the chamber and fill with Single distilled water no purer than 1 Mega Ohm.
It is recommended to fill the pan to about 1/2 inch below the top rim. See Sections 3.1 and 8.2 for more specifications and operational details.
7.8 Removing packing foam from under Air pump:
Remove back access panel
Remove 2 back access panel screws
7.9 Correct Installation
When the Incubator is installed correctly connected to the power source it is rated for gas connections made properly, the water pan filled, the shelves in place, and the unit is leveled. Read section 8 and follow all instructions for setting set points at the desired value. Then calibrate the Incubator control systems as explained in section 9. If the Incubator is installed and calibrated correctly it will meet the performance specs listed in section 3.2.
7.10 Reversing Incubator Hinges
Reversing the incubator hinges from the right to the left side SEE PTB0236 for instructions.
This requires disassembly of the incubator and should be done by a qualified service technician.
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Remove pink packing from under air pump indicated by the red arrow and store for possible future use
8.0 DH Operation
The Incubator is designed to provide a sterile, constant temperature, constant CO
2
level and naturally humidified atmosphere for optimum growth of tissue cell cultures and other organisms requiring this precise environment. To operate the Incubator properly, the following parameters must be reviewed, carefully set, and/or prepared.
8.1 Sterility
The chamber environment is not selective. The growth environment is applied equally to all microorganisms (specimens and contaminates) within the chamber. Therefore, before placing any cultures in the Incubator, the shelves and sidewall top plenums should be sterilized. The interior sidewalls, top, bottom, door, as well as the gasket should be wiped clean with a 70% solution of isopropyl alcohol or other disinfectant compatible with the chamber construction, to remove any contamination. Use mild detergent to clean the exterior of the Incubator.
8.2 Humidity
Humidification of the Incubator chamber is achieved through the process of water evaporation (vapor water pressure) from a stainless steel water pan placed on the bottom of the chamber. Materials of different thermal resistance (e.g., glass, plastic) do not offer sufficient thermal recovery and are not recommended for water pan usage. Although some metals offer better thermal coefficients than stainless steel, dissimilar metals cause electrolysis in the acid atmosphere (carbonic acid) and should never be used, or placed within the chamber.
Use only distilled or mineral-free water, no purer than 1 mega ohm, in the stainless steel pan. The water should be changed at least once a week. FLOODING THE BOTTOM OF THE INCUBATOR IS NOT RECOMMENDED since it is difficult to change the water weekly and almost always necessitates the use of chemicals, which are not recommended and may damage the stainless steel. Also, it promotes condensation on the inner walls because it steals the natural convection, heat flow through the inner chamber and condensation points occur.
Note: ABSOLUTELY NO CHLORINATED OR HALOGEN MATERIALS ARE TO BE USED IN THE CHAMBER.
Humidity recovery to 90% of original level will occur in 20-40 minutes on average after a 15-second door opening with a water pan surface area of 120 square inches (774.2 sq. cm). Contamination in the water pan may be avoided by adding a small amount of copper sulfate to the water pan after each Incubator decontamination. NOTE: Please contact the copper
sulfate manufacturer to determine the proper amount of copper sulfate that should be added to the water pan.
Condensation on the glass door, walls, top, or bottom of the chamber indicates an incorrect balance of door and front perimeter heat. Both the door and front perimeter heaters operate on a duty cycle. A good starting point for these duty cycles is the default setting for the door & perimeter heaters in a room ambient temp of 22°C (72°F) at a temperature set point of 37.0°C. The fresh airflow that the air pump delivers to the chamber has been preset at the factory. If condensation starts forming on the sides or back wall in the chamber, the number and length of air injections into the chamber can be increased. (See section 8.8.1.2 for instructions.) To alleviate the condensation, increase the airflow. To increase RH in the chamber reduce the airflow. If airflow is decreased, some condensation may be unavoidable. The port plug with breather hole must be used on the back port.
Note : DO NOT SEAL THE OPENING IN THE PORT PLUG EXCEPT WHERE INSTRUCTED TO DO SO.
THIS IS A PRESSURE RELIEF FOR THE CHAMBER DURING THE HEATED CYCLES.
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8.3 Control System Introduction
The NuAire Incubator Control Electronics system is designed to serve the control requirements of the Incubator chamber.
Temperature and CO
2
levels are controlled by preset values to provide the optimum conditions for culture growth within a chamber. Operator input is coordinated through the NU-TOUCH LCD keypad and status displays.
Figure 1 shows the various inputs and outputs of the system.
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer based system that provides:
8.3.1 Chamber control in a single electronic package.
8.3.2 Enhanced information presentation on a Nu-Touch LCD
Main Screen Continuously displays:
• Measured Chamber Temperature
• Measured CO
2
level in the chamber
• Current Screen ID “MAIN SCREEN”
• A mode status indicator (RUN or STANDBY)
• NuAire Logo button Leads to the System settings
(Or Password) menu when pressed
• Date and Time
• Air, CO
2
inject, door ajar status displays.
• An Alarm Status Menu
8.3.3 Active buttons on the MAIN SCREEN:
Button Description
Control system description
(i.e.….TEMP or CO2)
Measured value of each control system
Action when touched
Brings a pop up screen that describes the control system that was touched
Goes to the Environmental Set Point Screen
(Key pad to enter password will pop up if the password function is enabled)
Goes to “System Settings” Screen
Goes to screen for adjusting time and date
NuAire Logo
Date & time display
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8.3.4 Accessing the Incubator Control Screens
Accessing the many Control screens, performing functions and diagnostic tests, and maneuvering through the
NU-Touch LCD menu system is accomplished through the following methods:
• Touching a “graphic” Icon like the NuAire Logo in the Main Screen which access the System Settings menu, or lead you to an Enter Password screen when a password has been established.
• Touching a “Button function” button command that is visible on the screen.
The button will have text in it indication the function you are about to perform.
The following buttons are used on the NU-5800:
An explanation of these buttons is as follows:
Save –
Exit –
Close –
This command saves the changes you have made and returns you to the calling screen or, in some cases, back to the main menu.
This command exits you from the current screen and returns you to the calling screen.
The command causes the current screen to be removed and returns back to the calling screen.
Cancel – This command will cancel the changes you have made, and in some circumstances, cancels the “set of requests” that you have entered and returns you back to the calling screen
(e.g., Cancel in the middle of some of the CO2 zero/span screens returns you to the calling screen).
Start – This command causes a main event to begin (e.g. CO2 zero/span calibration).
Next Step – This command causes another step (screen), in a list of steps (screens) to be displayed
Back –
(e.g., some CO
2
calibration screens).
This command returns you to the previous screen.
• Touching a “Text” button command. The text or numerical value will be in blue indicating that is a link similar to the computer or on the internet. The system settings menu screen shown below is a good example of both the Text commands and Button commands:
Note: The Main Screen is the one exception for the above described buttons.
The green clock text is and is a link to adjusting the time.
The large white Control system measured values are also a “second” link to the “Environmental
Set-Points” menu and the control system descriptions are a link to system description pop up screens when touched.
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8.3.5 Functioning accessed from the System Settings screen:
The “System Settings” menu screen can be accessed from the “Main Screen” by touching the NUAIRE logo:
Environmental Set-Points: Allows adjustment of Temperature, CO2, Door, and Perimeter heater power level
settings.
Note: You can also access the Environmental set points by touching any control system numerical value
in the MAIN SCREEN.
Performance Monitor: Views a function’s Set-Point and Measured values over the last seven days.
Service Settings: Protected by the password 9876, it facilitates Calibrations, Option settings, and access to Diagnostics.
System Information: Views the units Model Number, Serial Number, Control board assembly (CCBA)
Software Revision and the Display board assembly (LCBA) Software Revision.
Display Settings: Allows adjustment of the screen brightness.
Set Password: A 4 digit password can be set and will protect access to Environmental Set points, and changing the password
System Settings Screen
8.3.6 Automatic notification of abnormal situations ( See Section 11 for more detail)
The red “Alarm Status” text message (button) will appear on the
Main Screen to indicate a fault within the Incubator system. An audible alarm is also heard. The audible alarm is on for 10 seconds at the beginning of an alarm condition and thereafter, it will be on 1 second every 30 seconds.
By depressing (touching) the Alarm Status text on the Main Screen, you now reach the Incubator Alarm Status screen which will identify which Incubator sub-systems are in an alarm state (Temperature,
CO2, or General). You may exit the screen or touch any of the subsystem buttons which will give you more specific information on alarm conditions. See Section 11.0 for more specifics on alarm conditions.
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8.3.7 Password Protection
There are 2 levels of password protection.
1.
The permanent numerical password required for accessing the “Service Settings” menu screen.
This screen is accessed by pressing the “Service Settings” text Icon in the “System Settings” menu screen.
9 8 7 6
The functions in the “Service Settings” screen are intended to be used by qualified technicians to calibrate the control system sensors like Temperature and CO2, adjust optional parameter settings to customize incubator performance, perform system diagnostics and to Factory or Master reset for returning the incubator to Default settings.
2.
A selectable 4 digit numerical password that is entered by the Incubator user to prevent others that are not authorized to change the Incubator set points (CO
2
%, Temp), running conditions of the Incubator, or changing passwords. The password protection may be initiated by accessing the “Set Password” menu in the System Settings menu screen. Once initiated, a four digit number must be entered, then re-entered for verification.
8.3.8 Standard remote communication capability
The NU-5800 Incubator comes equipped with the following remote communication tools:
• USB Output of daily, weekly & monthly performance logs and an event log that indicates when alarms, door openings and power interruptions occur.
• 4-20 milliamp analog Output
• RS-485 2 way communications
• A USB method of updating the software
8.3.9 Diagnostic and calibration assists.
To access the Service Settings Menu, perform the following steps:
NuAire Logo → Service Settings → 9876 Enter. You are now in the
Service Settings Menu (screen). This screen provides you the capability to review and analyze numerous Incubator functions including the following:
• Individual analog inputs may be displayed to assist calibration.
• Individual outputs may be forced to an on or off condition.
• Individual digital inputs may be displayed.
• Calibrated and Uncalibrated values are displayed.
• All options may be individually tested.
8.4 Front LCD NU-Touch screen Descriptions
The system LCD touch screen has the following functions.
8.4.1 Front LCD touch screen Descriptions
The NU-TOUCH LCD allows the user to access a wide variety of unit configurations and diagnostic menus by touching the
NuAire logo icon.
The Main Screen displays:
• Power Interrupted message displayed when power is lost and then re-connected
• current Calibrated values of control systems that have been enabled
• Current operating mode (run or standby)
• Door Ajar, Alarms, and Maintenance notices
8.4.2 Inject CO
2
Status
On the bottom right of ‘Main’ screen, “CO2” text indicates when the CO into the chamber, the system is injecting CO
2 to maintain set point.
2 control valve is open and CO
2
is flowing
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8.4.3 Inject Air Status
On the bottom right of the Main screen “Air” text indicates that fresh air is currently being injected into the chamber.
8.4.4 Door Ajar Status
The door ajar white text message indicates when the inner glass door is not closed and latched; the inner glass door is open. The door ajar status is a result of the pressure switch (open/closed) located along the top middle of the front panel. After the door is closed, the text will display “Door Closed Delay” indicating the delay time (60 sec adjustable).
8.4.5 Alarm Status Notice
The “Alarm Status” button indicates is triggered when a timer for a maintenance reminder runs to zero. This text appears in red just below the NuAire Logo (paragraph 8.4.2 and 8.4.3). The “Alarm Status” button and audible alarm indicates the abnormality. A catastrophic temperature control condition will de-energize the safety relay and cause the chamber to cool below the set point. The unit will resume heating when it has recovered from the abnormality.
See section 8.3.5 and 11.0 for more details on Alarms.
8.4.6 Maintenance Required Notice
The “Maintenance Required” button indicates that a check for maintenance should be done. This text appears just above the AIR CO2 status messages (paragraph 8.4.2 and 8.4.3). This is a visual alert only no alarm will sound. Press the “Maintenance Required” button to go to the screen that will tell you what maintenance should be performed.
See section 10 for more detailed information regarding this Notice.
8.5 Rear Panel Detail
The rear control panel contains the following functions (BDC-15669)
8.5.1 Power Cord
The power cord is 6.5-foot (2 m) in length, type “SVT” molded plug, allowing for long life and easy cleaning.
8.5.2 Circuit Breaker
All control electronics are protected with a circuit breaker that may trip at 110% of load rating, but will trip at 145% of load rating in less than 2 seconds.
Should the circuit breaker open (pop-out button will appear), merely depress to reset.
8.5.3 CO
2
Inlet All models
The CO
2
inlet provides a fitting for vinyl tubing.
Be sure to follow the recommended inlet pressure to insure proper flow rates and consistent CO
2
percentage readings.
8.5.4 Power Switch
The power switch, located at the top of the rear panel, controls all power to the Incubator.
8.5.5 Communication Interface/Alarm
Three receptacle connectors are provided for direct field connection of a computer for 2 way communications and incubator monitoring/or output to a printer or other device only capable of receiving RS-485 digital information, dry contact remote alarm indicator, also 4 to 20 mA output to an analog monitoring device or system.
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8.6 Front Component Panel
The front component panel contains the following functions. See BCD-15180
8.6.1 CO2 Supply Filter
The CO2 supply filter is installed at the factory and should be changed following the instructions found in
Maintenance Section 10.
8.6.2 Air Inlet Filter - All models
The Incubator is provided with clear vinyl tubing and a 0.3-micron HEPA filter. This is a free air supply.
Note: DO NOT CONNECT TO A PRESSURIZED SOURCE.
8.6.3 Chamber sample port
The Sample port is provided for checking the CO2 level in the chamber to monitor the calibration the Sensor and determine if it need adjustment.
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8.7 Run Mode Operator Interactions
8.7.1 THE FIRST TIME THE INCUBATOR IS TURNED ON
• The NU-Touch boot up screen appears then shows a screen instructing you to “Read the manual and follow all instructions to properly install the incubator” o
Press the exit button then a thank you screen will appear. o
Press the “Continue” button then the unit will go to the “MAIN SCREEN”.
These screens will not show again unless you “MASTER RESET” the incubator control board.
• Setting the Clock
8.7.1..1 Clock display organization:
YYYY-MM-DD Hr:Min and is displayed in green text in the lower left of the display screen.
8.7.1..2 Press the green text and the Time and date adjustment screen appears.
8.7.1..3 Use the up/down arrows to increase or decrease the highlighted value.
Use the left/right arrows to move between date and time values that require changing.
8.7.1..4 Press “Save” to save the changes you just made or
Press “Cancel” to abort the changes and return to the main screen.
The mode display, on the ‘Main’ screen, is used to identify the mode of chamber operation: either standby or run.
In general, there is no need for operator interaction in "run" mode. However, operator interaction is required when the incubator is first turned on then to perform calibration functions and address abnormal conditions status. If an abnormal condition has, or does, exist for a particular parameter, an alarm becomes active and the ‘Alarm Status’ button will appear.
This could be a catastrophic alarm condition, which could harm the tissue culture cells. It does indicate an operational abnormality and should be checked. To acknowledge the abnormality, simply press the “Alarm Status’ button to view the alarm, to dismiss it press the corresponding alarm’s button. The audio and visual alarms will then extinguish. If the abnormality still exists, the return after waiting the specified alarm time.
Let the Incubator run normally, and if the alarm doesn't come back on, then everything is normal. If the alarm does come back on, use the troubleshooting guide to correct the abnormality (see Section 11.0).
8.8 Standby Mode Operator Interactions
The NuAire incubator automatically enters the standby mode when necessary. There is no action required by the user to enter this mode. All functions that put the incubator in standby mode automatically start a 10 minute timer. If the function is not interfaced through the screen on the NU-Touch display the incubator the returns to the MAIN SCREEN and switches to RUN mode.
8.9 Diagnostic and Checkout Procedures
The Incubator controller provides general diagnostic facilities:
• Diagnostic mode is intended for factory and field technicians.
It allows them to turn the controller's output signals (heaters, valves, safety devices etc.) on and off.
• Diagnostics are located in the “Service Settings” menu categorized by the control systems (i.e. Temperature, CO2,
RH and O2) plus a general category for diagnostics not directly related to the control systems.
Press the “Diag” button to access them.
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8.9.1 Diagnostic Mode
The diagnostic mode allows the operator to configure and/or check the Incubator for input/output signals manually and individually.
To initiate the diagnostic mode, perform the following from the ‘Main’ screen:
• Select the NuAire Logo to enter “System Settings”
• Select “Service Settings” and enter 9876, and then hit enter (ENT)
• In the Service Settings menu, select the “Diagnostics” button for the desired function
Once entered, the Diagnostics screen will display the calibrated and uncalibrated values of the given function and all related components. There will be several buttons in this screen. Interacting one of these buttons will activate that function until the twenty second timer on the bottom edge runs to zero. Activating more than one function will reset the timer but both functions will remain on.
Output/Input Diagnostic Parameter Functions
Temperature
1.
Safety Relay (on/off)
2.
Chamber Temp. Sensor (shows current temp.)
3.
Safety Temp. Sensor (shows current temp.)
4.
Chamber heater Triac control (0 - 50% - 100 % phase firing)
5.
Door Heater Triac control (0 - 50% - 100 % phase firing)
6.
Perimeter Heater Triac control (0 - 50% - 100 % phase firing)
CO2
2
Sensor (Display current CO
2
percentage) 1.
CO
2.
CO
2
Inject Valve /on/off
3.
CO2 Sensor bay Heater Triac (0 - 50% - 100 % phase firing)
4.
CO2 Sensor bay Temp. Sensor (Displays current temp.)
5.
Air Inject Solenoid (on/off)
6.
Blocking Solenoid (on/off)
General diagnostics
1.
Power Supply Regulated +12 VDC (current voltage)
2.
Power Supply Regulated +5 VDC (current voltage)
3.
Air Inject Solenoid (on/off)
4.
Alarm dry contact Relay (NO/C/NC) switch states
5.
Air Pump Triac (on/off)
6.
Spare AC Triac
(Note: Default values are in bold)
The paragraphs on the following page provide a more complete description of the diagnostics listed above.
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Temperature Control Diagnostics
1. Safety Relay
This function shows the current state of the safety relay. LCD display will show “On” or “Off” corresponding to the relay condition. On for closed or set or yes.
2. Chamber Temperature Sensor
This function shows on LCD the current values, calibrated (displayed) and not calibrated of the temperature sensor controlling the chamber temperature.
3. Safety Temperature Sensor
This function shows on LCD the current values, calibrated and not calibrated of the Safety temperature sensor located in the chamber.
4.
Chamber Heater Triac Control (0%, 50%, and 100%)
This function allows the jacket heater to be turned on at different percentages 0, 50,100)
5. Door Heater
This function shows on LCD the current state of the door heater. This function also allows the door heater to be turned on at different percentages (0, 50, and 100 percent). The LCD display will show phase firing percentage chosen default is 0%.
6. Perimeter Heater
This function shows the current state of the perimeter heater. This function also allows the perimeter heater to be turned on at different percentages (0, 50, and 100 percent). The LCD display will indicate percentage of phase firing chosen default is 0%.
CO2 Control Diagnostics
1. CO2 Sensor
This function shows the current CO2 sensor reading.
The value comes in as a ppm number which can then be easily displayed as a percentage number
2. CO
2
Inject Valve
This function shows on LCD the current state of the CO2 inject valve. LCD will show “ON” or “OFF” corresponding to the valve condition. On for open or set or yes.
3. CO2 Sensor Bay Heater Triac Condition
This function shows on LCD the current state of the CO manually.
4. CO2 Sensor Bay Temperature Sensor
2
Heater triac. LCD will show 0, 50, and 100 percent corresponding to the relay position. The triac can be toggled to each percent of phase firing
This sensor shows the sensor bay temperature in degrees C
5. Air inject Solenoid
This function shows on LCD the current state of the Air Inject Solenoid.
LCD will show “ON” or “OFF” corresponding to the solenoid position.
Default is off-solenoid activates when the button is touched
6. Blocking Solenoid
This function shows on LCD the current state of the Blocking Solenoid.
Default is off – solenoid activates when the button is touched.
General Control Diagnostics
1. Power Supply Regulated +12 VDC
This function will display on the LCD screen the current value (+12.xx) of the regulated +12 VDC power supply.
2. Power Supply Regulated +5 VDC
This function will display on LCD screen the current value (+5.00) of the regulated +5 VDC power supply.
3. Chamber Air Inject Solenoid
This function shows on LCD the current state of the Air Inject Solenoid. LCD will show “ON” or “OFF” corresponding to the solenoid position. Default is off-solenoid activates when the button is touched
4. Remote Alarm Relay
This function shows on LCD the current state of the remote alarm relay. The LCD display will show
“ON” or “OFF” corresponding to the relay condition and its Normally Open (NO0 pair of contacts
5. Air Pump Triac
This function shows on LCD the current state of the Air Pump Triac. LCD will show “ON” or “OFF” corresponding to the relay position. The triac can be manually turned On and Off.
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8.10 Option Configuration Parameters
Format: Option (Default, Min/Max)
All the options for the NU-5800 Incubator use this same format. The default is set at the factory and can be restored in the field with a Master or a Factory reset command. On the following pages, there is a short description of each Option with its Default/Min/Max setting, followed by a more detailed description.
Temperature
1.
Temperature Max Above Set Point (1.0°, 0.0/50.0)
2.
Temperature Time Out Timer (360min, 0/500)
3.
Temperature Min Below Set Point (0.5°, 0.1/25.0)
4.
Temperature Sensor Differential (4.0°, 0.0/25.0)
5.
Temperature Recovery Delay Time (60 sec, 0/500)
CO2
1.
CO
2
System Enable (On, On/Off) (in Settings Screen)
2.
CO2 Max Above Set Point (1.0%, 0.1/25.0)
3.
CO2 Time Out Timer (30min, 0/500)
4.
CO2 Min Below Set Point (0.5°, 0.1/20.0)
5.
CO2 Inject Delay Time (40sec, 0/500)
Ge neral
1.
Door Close Delay Time (60 sec, 0/500)
2.
General Air Inject Time (5sec, 0/500)
3.
General Air Inject Cycle Time (1min, 0/500)
4.
General Digital Output Interval (0min, 0/300)
5.
General Door Ajar Alarm Delay (5min, 1/60)
6.
Settings (top of the screen)
Note: Selecting any of the Option title texts will open a brief description of that option’s functions.
(Note: Default values are in bold)
The following paragraphs provide a more complete description of the diagnostics listed above.
Temperature
1.
Temperature Maximum Above Set point
This value determines the maximum deviation, measured in (°C) that the chamber is permitted to rise above set point, once it is reached before an alarm condition is declared. An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified.
2.
Temperature Time Out
This value determines the time, in minutes, for the temperature to achieve set point. If the temperature doesn't get to within 0.2° of set point within this time period, an alarm condition is declared.
3.
Temperature Minimum Below Set point
This value determines the maximum deviation, measured in (°C) that the chamber is permitted to fall below set point, once it is reached before an alarm condition is declared.
4.
Temperature Sensor Differential
This value specifies a maximum differential, measured in temperature (°C) that the two temperature sensors may deviate from one another, or from the last read value. If this differential is exceeded, a warning LED is shown on the running chamber. If multiple sensors fail to read within the specified limits, an alarm condition is declared. An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified.
5.
Temperature Recovery Delay Time
This value determines the time, in seconds, to turn off the chamber heaters in half-degree increments during a temperature recovery cycle. The delay time is required to prevent temperature overshoot of the control set point.
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CO2
1.
CO2 System Enable (Enabled/disabled) (Settings Screen)
This function will enable or disable the CO2 system. If the system is Enabled it will be displayed in the
MAIN screen. If it is disabled it will not be displayed.
In the Option/Settings screen for the CO2 system:
System Enabled (with check mark) means the System is enabled
System Enabled (without check mark) indicates that the system is disabled
2.
CO2 Max Above Set point
This value determines the maximum deviation, measured in (%) that the chamber is permitted to rise above set point, once it is reached before an alarm condition is declared.
3.
CO
2
Time Out
This value determines the time, in minutes, for the CO
2
percentage to achieve set point. If the CO
2 percentage doesn't get to within its lower limit of set point within this time period an alarm condition is declared
4.
CO2 Min Below Set Point
This value determines the amount in % below the CO2 set point that the Time out timer will start.
5.
CO
2
Inject Delay Time
This value specifies the time, in seconds, for an injection of CO
When CO
2
2
to be measurable at the sensor.
is injected into the chamber, the system delays until this time period has elapsed before making a new control decision.
system to overshoot the control set point. In this manner, diffusion induced delays do not cause the CO
2
General Options
1.
Door close Delay Time
This value determines the time, in seconds, to turn on the door heater to a 50 percent duty cycle after an
control for the same period. inner glass door opening. Also inhibits CO
2
2.
Air Inject Time
This value specifies the time, in seconds, for an injection of air into the chamber
3.
Air Inject Cycle Time
This value specifies the amount of time in minutes between each injection of air into the chamber
4.
Digital Output Interval
This parameter specifies the frequency, in minutes that lines are to be printed on a status report.
If the frequency is specified as zero, no report will be printed.
5.
Door Ajar Alarm Delay
Determines how long in minutes the glass door can be open before an alarm is declared.
6.
Settings (top of screen)
Pressing the Settings text gives access to Settings screen containing the following:
• The Filter Maintenance button to access:
• Resetting the change filter notice
• Setting time to reminder (24 months/1/48) 0 disables the feature.
• View time remaining until current notice is triggered.
• The Clear Password button that clears the 4 digit password that was input through the Set
Password screen.
• User screens language to English. Spanish, French & German are available see section 8.11.3 for instructions to change the user screen language.
8.11 Resetting the Electronics Software
8.11.1 Factory Reset
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The Factory Reset is similar to the Master Reset. However, it only changes the Options (returned to default settings) and the environmental set-points (returned to their defaults).
To perform a Factory Reset, you will need to do the following:
NuAire Logo → Service Settings → 9876 Enter → Reset → Factory Reset → Confirm
The Incubator will perform the Factory Reset and return to the Main screen.
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8.11.2 Master Reset
The master reset diagnostic function is the last effort to correct operational faults which otherwise cannot be solved. By reloading the default configuration, the entire memory will be reset and ALL CALIBRATION OFFSETS,
USER PASSWORD, CONFIGURATION OPTIONS, MODEL NUMBER OF THE INCUBATOR, AND THE SERIAL NUMBER
WILL BE LOST. All calibrations will need to be performed following a master reset.
To perform a Master Reset, you will need to do the following:
NuAire Logo → Service Settings → 9876 Enter → Reset → Master Reset → Confirm
The Incubator will perform the Master Reset then the “Factory Setup” Screen will appear
Note: After performing either Factory or Master Reset remember to turn the incubator off
then on again to ensure that all intended parameters were reset.
8.11.3 Inputting the Factory settings:
The “Factory Set-up” screen will appear after the master reset, this screen has several functions.
Setting the model number sets the following function:
• Model NU-5800 and NU-5800E or NU-5800D
Enables Temperature and CO2 the only control systems available on this model
Chooses the algorithm suited for low watt heating of the chamber
Supresses the decon button in the “Systems Settings” screen
Loads the model number in the System information screen
• Models NU-5810, NU-5820, NU-5830 & NU-5840 and E or D models
Enables Temperature and CO2 plus RH and O2 control systems as available on the various models
Chooses the algorithm suited for high watt heating of the chamber suited for heated Decon cycles.
Activates the heated decon cycle choices in the “Systems Settings” screen
• Touch the “Model Number” text then follow the screen prompts as shown below.
Pressing continue on the choose Region screen will bring you back to the Factory Set-Up screen showing the Model Number you just entered. Default is NU-5800 if no model number is entered
• Touch the Serial Number text then enter the 12 digit serial number which can be found on the serial number label located behind the hinged valence under the sample port.
Press the ENT button on the key pad screen and you will return to the Factory Set-Up screen showing the serial number you just entered. Default is blank if no number is entered.
NU-5800
• Touch the Set Language text then touch the save button. This sets the language on all of the user screens.
The language in the Service Settings menu /screen will remain in English.
English is default language if none is chosen. Touch the green save button to exit the factory screen.
NU-5800
123456789123
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• The final change that is requested is the clock. It should not have been reset.
As long as the date and time are correct you can cancel out of that screen to the Main screen.
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9.0 Calibration
Proper calibration of the incubator involves four parameters: chamber temperature, door temperature, perimeter temperature, and CO
2
sensor. The first three, chamber, door, and perimeter temperature should be completed and stabilized before any CO
2 sensor calibration is performed. Below, each calibration procedure is described in detail. For the best results, follow the procedure carefully, and if the desired result is not achieved, try procedure again from the start.
9.1 Chamber Temperature Calibration
The DH’s TEMPERATURE CALIBRATION MUST BE PERFORMED WITHIN 1°C OF THE PLANNED OPERATING TEMPERATURE.
Normally, 37.0°C is the most common set point. To initiate the procedure, turn on the incubator via the power switch on the back panel. The default set point is 37.0°C; enter “Environmental Set Points” and adjust if desired Let the unit stabilize for 8 to 12 hours. Use an independent calibrated instrument to check and calibrate the temperature.
Below is a description of calibration with a glass thermometer as one example for temperature calibration.
At the beginning of this procedure, set a calibrated glass thermometer in a glass beaker filled with water resting on a shelf in the middle of the chamber. Do not place the glass beaker on the bottom of the chamber because it will result in a slightly higher temperature reading due to the heater located on the chamber bottom. Placing the thermometer in glass beaker on the middle shelf will give the most accurate results for calibration. The chamber should be humidified to avoid false low readings due to evaporation of water from the flask. An accurate digital thermometer with a type K thermal couple could also be used.
When the unit has stabilized at the operating temperature, perform the following calibration procedure.
• Allow Incubator to stabilize at its given temperature set point in run mode.
• Enter “Service Settings” by means of your provided password.
• Press the Temperature Calibration button.
• Determine actual temperature within chamber by reading temperature measurement instrument.
• Select the number digits below the “Current Calibrated Sensor Reading” blue text button
• Input the current value displayed on your temperature measurement instrument and exit calibration.
Note: THE DISPLAY DOES NOT UPDATE IMMEDIATELY; IT TAKES 2-3 SECONDS
The chamber temperature calibration is complete. Let unit stabilize for 8 to 12 hours. If the chamber temperature (actual thermometer) still does not match the display, perform the above procedure again. In some cases it might be necessary to calibrate several times to achieve a stable condition due to ambient conditions of temperature and humidity within the laboratory.
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9.2 Door and Perimeter Duty Cycle adjustments for Temperature Calibration
The Door and Perimeter heaters are run on “Base Duty Cycle” calculations that is automatically increased or decreased to run in accordance with the chamber temperature at a chosen set point. These duty cycles work in the controlled lab environment but due to the many variables involved in temperature control there is also a manual adjustment. The heater duty cycles can be adjusted to control condensation or to adjust the temperature uniformity in the chamber.
The inner glass door and outer shell perimeter temperature calibration is best accomplished by running the Incubator for a minimum of 24 hours with the water pan in place and perform the following calibration sequence, if required. Open the
Incubator door and look for general condensation. Some condensation on the glass door can be desirable as an indication of adequate humidity in the chamber. Typically, one to two inches of condensation in the corners of the glass door and/or gasket indicates a properly calibrated door and perimeter heater. The perimeter heater is the heater located on the outer shell next to the glass door gasket. If calibration is required, simply perform the procedure as stated below. The door and perimeter heater operates as a base duty cycle calculated for the chamber temperature set point being used. In the door and perimeter heater (adjustment) screen “100” = the calculated base duty cycle. Adjusting the value up or down adds or removes 1 percent of the base duty cycle based on phase firing the control triac for that heater. Adjustments should be limited to 5% up or down at a time.
The following steps should be taken for setting these duty cycle percentages:
• Allow Incubator to stabilize at its given temperature and humidity level in run mode.
• Enter the “Environmental Set Points” screen either from the MAIN SCREEN or the SYSTEM SETTINGS screen.
• Select the numerical digits following the “Door” or “Perimeter” text.
• Press ▲ or ▼ keys to increase or decrease the numerical value to the desired level.
• Press “Done” to set current value and exit calibration.
Environmental Set-Points
Temperature Set-Point
CO2 Set-Point
Heaters, Door
Exit
100
37.0C
5.0%
Perimeter
100
9.2.1 Door and Perimeter heater duty cycle automatic control
The door and perimeter duty cycles are automatically reduced when the room temperature in the lab increases enough to allow the contribution from these heaters to overheat the chamber. For example if the door and perimeter duty cycles are set up when the room temperature is 22°C and the room temperature is allowed to increase to 27°C. Less heat is required to keep the chamber at set point. If the chamber starts to overheat, the duty cycles will be reduced at a rate of 1% per minute starting when the chamber temperature is 0.2°C above set point.
The duty cycles will continue to be reduced until the chamber temperature returns to set point. These duty cycles are continuously monitored and will be increased slowly again, as long as the chamber temperature does not go over the set point. If the room ambient reduces back to 22°C the door and perimeter duty cycles will actually be returned to their original settings.
Note: If it is known that the lab room temperature where the Incubator is installed will vary significantly.
(For example, the heater or air conditioning is shut off after work hours or there is no air conditioning and the room temperature has large temperature swings.)
The door and perimeter duty cycles should be set in the lower temperature expected in the lab.
Then the door and perimeter heaters will automatically be adjusted to avoid over temperature conditions in the chamber when the room temperature rises.
In this case the chamber should be monitored for condensation regularly.
If the chamber walls and ceiling start to get excessive condensation the door and perimeter heater duty cycle settings will need to be reduced.
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9.3 Setting Air Injections
If there is still some undesired condensation in the chamber when the door and perimeter heaters are set for the desired result, the air injections can be adjusted. There is a control for length of the air injection labeled, Air Inject Time, and the frequency that air is injected called, Air Inject Cycle. These controls are described in more detail in the "General Options" section of the Service Settings menu. The default is 5-second injections every 1 minute. Start by increasing the length of the injection by a few seconds at a time then increase the frequency if needed. If the humidity in the chamber is less than desired, reduce the Air Inject Time.
9.4 CO
2
Calibration
The DH infrared CO
2
sensor may be calibrated using one of four techniques: Open Door CO
2
, Closed Door CO2, Off-Set, and
CO2 injection calibrations. CO2 calibrations can be performed in approximately ten minutes, by accessing the CO2 calibrations menu in “Service Settings” and following the on screen instructions. To perform Off-Set, Open Door, and Closed
Door calibrations attach an independent CO2 measurement device to the Sample Port in the front of the unit before the calibration begins.
9.4.1 CO
2
CO
2
Off Set Calibration
Off Set Calibration can be performed anytime an independent measurement doesn't correlate to the front panel display. However, this calibration SHOULD NOT BE PERFORMED MORE THAN ONCE PER WEEK. Sensor calibration should be performed if an independent measurement, if it doesn't match the display within +0.3 percent within one week after a sensor calibration. Before doing the following calibration, check and change, if necessary, the Incubator in-line filter found within the Sensor bay.
When unit has stabilized at the operational temperature and CO
2
percentage, take an independent measurement and, if necessary, perform the following:
• In “Service Settings” enter the CO2 Calibrations menu.
• Press the “CO2 Offset Calibration” text button
• Use an independent instrument to determine actual CO independent measurement).
If these two readings have a difference of less than 1.0 percent, proceed to enter the CO
2
Fyrite value.
(See * Note below)
If the difference is greater than 1.0 percent, it is recommended to perform a CO2 Open Door Calibration.
• Enter the value measured by the independent device
• Exit and Save to retain this value.
2
percentage (compare the display CO
2
to the
*Note: When the display value is more that 0.3% different from the measured value, offset display
1/2 the difference measured.
Allow the Incubator to stabilize back to set point, then measure the CO
Offset the display again if necessary.
2 in the chamber again.
9.4.2 CO
2
Sensor Calibration (Zero/Span/CAL. INJ.)
There are 2 sensor (zero/span) calibration routines available to the lab professional. The first option is the “open door” routine involving opening the outer and inner door to zero the sensor. This routine also automatically calibrates to the CO
2
injection rate during the injection for the span portion of the sensor calibration. It is recommended that this routine be used during the initial setup of the Incubator, if the set point of the system is changed or if other changes are made on the Incubator affecting the CO
2
system.
The second option is a “closed door” routine. This routine allows calibration of the sensor without opening the door avoiding undue exposure to the cultures that may be in process. This routine injects “fresh air” into the detector head of the sensor to calibrate zero. The chamber air is then allowed back into the detector head to calibrate the gas span that is detected.
9.4.3 Open Door CO
2
Sensor Calibration Routine
The CO2 inject rate is automatically calculated from the CO
2
injection made during the span calibration making it unnecessary to run the separate “Injection Calibration”. The injection is dependent on the proper gas pressure and factory set flow rate. Any changes in either will result in a change to the value reached during this injection.
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9.4.4 Closed Door CO
2
Sensor Calibration Routine
• Attach independent CO2 monitoring device to the sample port on the front of the unit
• Enter CO2 calibrations by means of Service Settings
• Select the “CO2 Closed Door Zero Span” text
• Follow on screen instructions
Enter measurement on independent device when prompted
Allow unit to run and stabilize for a minimum of 2 hours then, check calibration with an independent instrument.
Compare the display CO
2
percent to your independent measurement. If these two readings have a difference greater than 0.3%, repeat above procedure. If these two readings have a difference of less than 0.3%, perform the
CO
9.4.5 CO
2
Offset calibration procedure in Section 9.4.1.
2
Injection Calibration
The CO
2
injection calibration can be performed separately from zero/span calibration to optimize the gas injection time required to recover the CO
2
level to set point after a door opening. The recovery time should be as minimal as possible with virtually no overshoot. Verify the CO
2
sensor calibration prior to performing an injection calibration.
The injection calibration is not required after an “Open Door” sensor calibration since it is performed automatically during this routine. The injection calibration should be performed after a “Closed Door” sensor calibration when possible, if CO
2 supply pressure of the Incubator is changed, or is the CO
2
flow control valve is disturbed.
The following steps should be taken for the CO
2
injection calibration:
• Enter CO2 Calibrations by means of the Service Settings menu
• Select the “CO2 Injection Time” text
• Follow the on screen instructions
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10.0 Maintaining Your DH
DH Chamber
The chamber maintenance is up to the discretion of the owner and the extent of cleanliness and sterility desired.
The shelves and bracket supports are all removable and autoclavable.
The interior should be wiped down with an appropriate disinfectant such as 70% ISOPROPYL ALCOHOL or equivalent.
Note: DO NOT USE ANY CHLORINATED OR HALOGEN MATERIALS IN THE CHAMBER.
SUCH MATERIAL IS HARMFUL TO THE POLISHED STAINLESS STEEL.
The humidity pan should also be sterilized and the water changed regularly to assure sterility.
A small amount of copper sulfate may be added to the humidity pan to inhibit bacterial growth.
CO
2
Supply Filter
P/N X-980385 (50 mm Disk, Uni-directional In-Line, Dry)*
The CO
2
Supply Filter should be replaced every fifth empty CO
2
tank or when the filter is visibly discolored (yellow-brown).
Sensor Bay Capsule Filter
P/N RF566 (Capsule, Uni-Directional In-Line, Wet)
The CO2 Sensor Filter should be replaced EVERY TWO YEARS to assure optimum performance.
A visual check should be performed during CO2 sensor calibration to assure filter integrity.
Remove sensor housing in back of unit cover to perform visual check.
See section 10.3 for automatic notification for checking this filter.
Outlet port is on flat top side.
Air Supply Filter
P/N X-980385 (50 mm Disk, Uni-directional In-Line, Dry)*
The Air Supply Filter should be replaced every fifth empty CO
Air Pump Filters
P/N X-980366 (50mm Disk, Uni-Directional In-Line, Wet)*
2
tank or when the filter is visibly discolored (yellow-brown).
The Air Pump Filters should be replaced EVERY TWO YEARS to assure optimum performance.
A visual check should be performed during CO
2
sensor calibration to assure filter integrity.
Remove the sensor housing cover to perform visual check.
* Note: The word "In" on the outer rink of the body indicates the inlet side of the filter and should be installed toward the gas supply.
10.1 Chemical Decontamination of the Incubator Chamber
To chemically decontaminate NuAire Incubator inner chambers, users may use the traditional formaldehyde or Chlorine
Dioxide in gas form, or Vapor Phased Hydrogen Peroxide.
All three of the chemicals are compatible to all parts within NuAire Incubator chambers.
The incubator should be turned off during these procedures
Note: As stated previously, the chamber and components can also be wiped down with a
70% solution of Isopropyl Alcohol for cleaning and decontamination.
10.2 Shutting down the Incubator
Prior to shutting down the Incubator open the inner and outer doors and remove the water pan.
Leave doors open for at least 5 minutes prior to shutting it off.
This will purge the chamber, circulating system and the sensors of humidity that could condense and cause faulty readings when the Incubator is turned back on.
Be sure to empty the water pan prior to putting it back into the chamber if the Incubator is going to be shut off for any length of time.
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10.3 Automatic Filter Change Notification
The Capsule filter located in the Sensor bay (see BCD- 15634 for location) should be checked average every 2 years to see if it should be changed. The
Filter material is normally a white material. Discoloration of the filter material is an indicator that it should be changed. Retro Fit Kit RF566 should be ordered for the replacement filter. A timed notice triggers a Maintenance Notification button in the Main Screen when this timer counts down to zero. Pressing the
Maintenance Required button produces a screen indicating that the timer for filter life has expired.
Responding to the notification:
From the main screen press the NuAire Logo / Service settings / General
Option / Settings / Filter Maintenance to access the Filter Maintenance
Adjustments screen. In this screen you can extend the alarm by 1 month by pushing the Extend? button. Reset the notification timer from 1 month to 48 months by sliding the green button right or left on the scale. The number of months showing will change as the button is moved. Setting the timer to 0 months deactivates the notification. After extending or resetting the timer you can return to the main screen by following the screen prompts.
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11.0 Error Indicators & Troubleshooting
Step 1 NOTE ALL ERROR INDICATORS.
When the Incubator is running, the Alarm Status button indicates an error.
Pressing the Alarm Status button, entering the correlating Alarms Menu and pressing the Silence button in the Alarms
Menu will silence the audible alarm until Diagnostics is entered or another alarm becomes active.
Step 2 CLEAR ERROR INDICATORS.
Error indicators can be cleared by pressing the Alarm Status button and entering the Alarms Menu and pressing the
Clear All button; if the error is still present the indicator will return when the alarm time reaches 0.
Step 3 MONITOR REOCCURRENCE OF ERROR INDICATORS.
If reoccurrence of the error indicator is immediate or daily, use the Error Indicator Troubleshooting Guide on the following pages to address the issue indicated by the reoccurring alarm.
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Displayed Error Code
Temp Over Set Point,
Temp Under Set Point,
Failed Sensor Differential
Sensor Bay Temp Low
CO2 Over Set Point
CO2 Under Set Point
Cal Inject Failed
Door Ajar/rapid beeping
“Power Interuption” Text replacing NuAire Logo
Corrupted Memory
Data Write Failure
12 VDC Power Failure
5 VDC Power Failure
Zero Cross Failure
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Error Indicator Troubleshooting Guide
Code Description Checks and Corrections
-Sensor temperature (differential)
error normal running.
-Occurs when difference between
sensors exceeds 4°C.
Temperature System
-Temperature over setpoint
-Temperature time out error
during normal running and in
Check temperature sensor calibration.
Faulty TRIAC, replace control board.
Check temperature sensor calibration
Replace fuse.
Faulty TRIAC, replace control board
Faulty chamber heater contact NuAire Technical Service.
Door/Perimeter heater needs to be increased with a high
temperature set-point in a low ambient temperature
Check temperature sensor calibration
Check connection on control board
One or both temp sensors faulty, replace
CO2 Sensor Shroud Heater
-Low shroud temperature
Check control board output to relay, replace board if faulty.
Check heater relay for function, replace if faulty
Check both shroud heaters for function, replace if faulty.
CO
2
System
- CO
- CO
2
2
over setpoint
time out error
Perform CO
2
sensor calibration
Check injection solenoid for leaking valve
Check sensor and disk filter for condensation
Check CO
2
gas supply - inline gas filters, CO
2
gas tank
pressure, CO
2
sensor function
Run Cal Inj. Calibration (see Section 9.3.3)
Check/replace CO
2 gas supply tanks
Check for leaks in chamber
- inner door gasket, chamber blower fan shaft seal
Check for leaks in air pump and hosing
- Cal inject calibration failed.
Not enough increase in the CO
2
Check gas supply then run calibration again
Call NuAire Technical Services if error persists.
reading after gas was injected.
General Indicators
- Inner glass door is not closed or the
door switch needs attention.
Close and latch inner glass door.
Confirm placement on the Control Board
Check door switch, if faulty, replace.
- Interruption of power Press any key to dismiss the power interruption notice.
Corrupted memory was detected during boot up of the incubator
A change in parameters failed to write to memory
Low voltage detected on the 12 VDC power circuit
Low voltage detected on the 5 VDC power circuit
The Power Triacs control circuit not working
Replace Control Board
Replace Control board
Check output @ Power Supply-If good could be Control Bd
Check power supply connection to Control board
DC power supply failed - replace
Check output @ Power Supply-If good could be Control Bd
Check power supply connection to Control board
DC power supply failed - replace
Check for loose line voltage connection to control board
Replace fuse with slow blow fuse of the correct amp rating as specified on the wiring diagram
Replace Control Board
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Condensation Excessive
(Humidity pan in place)
- Glass door, gasket or front wall of
chamber is wet.
- Back wall bottom and top walls
Increase door and front perimeter heater duty cycles.
See section 9 for detailed instructions.
Decrease door and front perimeter heater duty cycle.
See section 9 for detailed instructions.
Note: Depending on the room ambient that the Incubator is operating, the operator may have to readjust the door and perimeter
heater duty cycle are too high and likewise if the door and perimeter heater duty cycles are too low the glass door and front
wall of the chamber will show excessive condensation.
Condensation Persists After
Door and Perimeter Duty
Cycles are Adjusted
Excess Vibration
Increase air injections, increasing duration first, recommended initial change to 45 sec (Air Inj)
& 10 min (Air Cyc)
Check for and remove the block of shipping foam from
under the air pump.
Remove top plenum and ensure that blower wheel is not
rubbing on anything.
Check jacket fan mounted in bottom on Incubator
For further assistance, call NuAire Customer Service at 1-800-328-3352 or (763) 553-1270 USA.
12.0 Communication Outputs
12.1 Remote Alarm Contacts
The NuAire DH Incubator contains a set of contact points to connect to a remote alarm system. The contacts are located on the rear panel (see page 14). The contacts are housed in a modular (RJ-11) telephone jack and rated for (30V at 1
Amp). The contacts provided are normally open (NO), normally closed (NC) and common (COM) as shown below. The alarm contacts do not distinguish between a CO
2
temperature and any other alarm. Each will open or close the contacts upon an alarm condition. Power interruption will also change the state of the contacts. To reset the alarm contents press the Run/Setup key to setup, then back to Run.
12.2 4 to 20mA Analog System Performance Output:
12.3 RS-485 2-way Communications:
12.4 On-Board USB Port:
Uploading Service Performance/Event Data:
Uploading Incubator Programming:
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Refer to PTB0235
Refer to PTB0234
Refer to STB0213
Refer to PTB0212
13.0 Electrical/Environmental Requirements
13.1 Electrical (Supply voltage fluctuations not to exceed +/- 10%)
NU-5800
NU-5800E
115V, 50/60Hz,
230V, 50/60Hz,
1 Phase,
1 Phase,
Start Up Power
Running Power
345 WATTS
175 WATTS
13.2 Operational Performance (for indoor use only)
6.0 Amps
3.0 Amps
Environment Temperature Range:
Environment Humidity:
Environment Altitude:
60°F-85°F (15.5°C – 29.4°C)
20% - 60% Relative Humidity
6562 ft (2000 m) over sea level maximum
13.3 Light Exposure
Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity.
13.4 Installation Category: 2.0
Installation category (overvoltage category) defines the level of transient overvoltage, which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means.
For example, in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply.
13.5 Pollution Degree: 2.0
Pollution degree describes the amount of conductive pollution present in the operating environment.
Pollution degree 2.0 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.
13.6 Chemical Exposure
Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting.
Chlorinated and Halogen materials are not recommended for use on stainless steel surfaces.
Chamber decontamination can be accomplished by paraformaldehyde, vapor phased Hydrogen Peroxide or Ethylene
Oxide without degradation of cabinet materials.
13.7 EMC Performance (classified for light industrial)
Emissions:
Immunity:
EN61326
EN61326
Class A equipment is intended for use in an industrial environment.
In the documentation for the user, a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments, due to conducted as well as radiated disturbances.
13.8 Heat Rejection: 10 BTU/Min.
!
WARNING
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Key Features
- Direct heat chamber
- Precise temperature control
- Microprocessor-based CO2 control
- HEPA filtration
- Large capacity (7 cu ft)
- User-friendly LCD interface
- Sterilization-ready shelves
- Adjustable humidity
- Remote alarm and communication outputs
Related manuals
Frequently Answers and Questions
What is the temperature range of the NU-5800E incubator?
How is humidity controlled in the NU-5800E incubator?
Can I sterilize the shelves and other accessories of the NU-5800E incubator?
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