N-BIOTEK NB203QR Operation Manual
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The N-BIOTEK NB203QR is a CO2 incubator with a built-in roller apparatus, designed for cell culture applications. It features accurate temperature and CO2 control, a natural humidification system, and a heated outer door to prevent condensation. The incubator offers a spacious chamber with multiple shelves and a 2-stair roller system for consistent cell growth.
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NB203QR Operation Manual CO2 incubator + Roller Model : NB-203QR 1 Table of Contents NO. CONTENTS PAGE 1 SAFETY AND GENERAL PRECAUTION 3 2 TRANSPORTATION, STORAGE, AND LOCATION OF INSTALLATION 5 3 PREREQUISITE AND CONFIGURATION 7 4 FEATURES AND SPECIFICATIONS OF THE UNIT 9 5 CONTROL PANEL 10 6 OPERATION 11 7 SAFETY SWITCH 16 8 ROLLER OPERATION 16 9 HOW TO DETACH THE ROLLER 17 10 TROUBLESHOOTING 18 2 1. SAFETY AND GENERAL PRECAUTION 1.1 General information on precaution ⚫ Precaution is to prevent the possible accident or danger during operation. So, you must keep it. ⚫ Precaution is divided into caution and warning. And, each of them has following meanings. If you don't keep this warning, you can get an accident or a fire. If you don't keep this caution, you can get injured as well as a property loss. 1.1.1 Safety warning symbols 1.2 Precaution for using the power cable Do not make the power plug be pressed by back of the product. (A space between the product and the plug must be 30cm at least.) The power outlet must be only for this product. (Using various products simultaneously can cause a fire.) Clean the power plug with a dry towel and connect it properly. (Foreign substances or unsafe connection can cause a fire.) Do not bend the power cable hardly and do not make it to be pressed by heavy products. (When it is damaged, it can cause a fire.) Do not touch the power cord with wet hands. (It can cause an electric shock.) Do not use the damaged power cord and outlet. (It can cause an electric shock and a fire.) When you see smoke coming from the product or smell burning, you have to pull out the power cord and stop using it. (It can cause an electric shock and a fire.) 3 1.3 Precaution for ground connection Please ground before use the product, if you don't ground, you could get an electric shock when malfunction or an electric leakage occurs. At the place where you can't ground, * Please buy the equipment to prevent any electrical leakage. * An electric shock, an electric leakage and a fire can be occurred without an electric leakage breaker. Do not ground to these places; Gas Pipe, water pipe, pipe, lighting rod, telephone wire, etc. * Wrong ground connection can cause electrical leakage which eventually results in fire. If you don't have the outlet for AC 220V, then bury it under the ground after connecting the ground line to copper plate. * No ground connection can bring an electrocution, an electric leakage and a fire. 1.4 Precaution for use You must not disassemble, fix and remodel the product by yourself. (You can damage the product throughout a fire and malfunction or get a property loss as well as experimental loss. Do not use the product for different purpose. (It can cause malfunction or poor function. Consequently, you will get a wrong result.) Do not use an inflammable spray near the product. (The switch and other electric connection parts can cause a fire.) When you use inflammable substances such as benzene, thinner, alcohol and LP gas, please be careful. (It can cause a fire and an explosion.) To prevent water and experiment material from going into the control panel during the experiment, make sure to clean the control panel with a dry cloth. (It can cause an electric leakage and a fire.) Do not wash the product with excessive quantity of water, thinner, benzene and Petroleum. (It can cause an electric leakage, and malfunction or damage on the surface.) 4 When you don't use the product or clean it, please pull out the power plug. (It is to prevent an eclectic leakage.) Open and close the door softly and please use a door knob. (A heavy shock can damage the product and breakdown the operating part. Also your hands can be stuck between the door and body.) Do not detach the built-in lamp and electrical devices. (It can cause an electric shock and a fire.) Please be sure to prevent foreign substances from getting into the sealing silicon of the door. (The inflow of open air can cause the change of temperature in chamber and discoloration of the packing part by a foreign substance.) 2. TRANSPORTATION, STORAGE, AND LOCATION OF INSTALLATION 2.1 Transportation DO NOT try to slide or tilt the unit Lift the unit on each corner of bottom with the aid of 2 people. Permissible ambient temperature range for transport: -10℃ to 60℃. 2.2 Storage Do not keep it at place in High Humidity. Permissible ambient humidity: max. 70% storage in a cold location is the place you transfer the unit to the installation site for start-up, condensation may form. In this case, Wait at least one hour until the CO2 incubator has attained temperature and is completely dry. Please check the voltage & Hertz written on serial label. (Over-voltage, under-voltage can damage the product and poor performance.) Do not install at a humid place. (It causes an electric leakage accident and a corrosive of the product.) Keep this product out of the direct ray of sun and do not install at a hot place or a place that is near an electric heat. (The proper indoor temperature is 20℃ ~ 30℃.) 5 2.3 Location of installation and ambient conditions Do not put flammable substances near the product. (It may cause a fire.) When you install the product, you are required to keep the distance at least 30cm from the wall. To completely separate the unit from the power supply, power plug must be disconnected. Install the unit in the way that the power plug is easily accessible and can be easily pulled in case of danger. Install the unit at a flat surface, free from vibration and in a well-ventilated location. (If the ground is not flat, it may cause an excessive vibration of the product.) When you move the product, do not lay down to its side or reverse the head to bottom. (It may cause a malfunction.) When you move the product, hold the door and other movable parts of the product with a tape. (When the product is moved, the movable door can cause injury of you and damage of the product.) When you move the product, you must hold up the product. (Pushing or pulling the product can damage the bottom part of the product.) 6 3. PREREQUISITE AND CONFIGURATION 3.1 Prerequisite Inspection of Boxes When you have received the instrument which is packed on pallet, inspect the box carefully for any damages that may have caused any damages to product during shipping. Please report any damage to the carrier or to your local N-BIOTEK distributor immediately. LOCATION The incubator is designed to operate at temperature 5℃ above ambient, and recommended to operate at minimum ambient (temperature in the place for use), 15℃. Maximum Room Temperature is 32℃. To avoid place for use this incubator is as below. 1. 2. 3. 4. 5. Near Heater or Freezer(if it may generate heat and affect temperature control of incubator) Near Equipment generating heat or cold air to incubator. Directly Sunlight Exposed to incubator Uneven ground or table head The place where is being vibrated Cleaning before use Before conducting cell culture, It is recommended to clean up entire chamber and shelves, water tray by using at least 70% Ethanol mixed of 30% distilled water and soft clothes. Inserting shelves Shelves are mounted onto the shelf racks in such a way that the edge of shelves which is slightly bent up about 8mm goes to the back of the chamber until it is too close to the back wall. Basically, 3 shelve are provided. Insert the shelves from top to bottom. Level the incubator by adjusting feet. Place a small level on the second shelf of the incubator adjust the levelling fee until the incubator is level and stable. After inserting the shelves, place humidity tray in the bottom of chamber if humidification is required for your application. 7 3.2 Configuration Exterior Configuration of CO2 incubator. <Figure #1> Control panel Air jacket -6 side direct heating Ventilation fan Power switch Glass door heater -Prevent condensation Magnetic door seal Stainless steel shelve - Perforated to improve uniformity Glass door Inner door seal - Airtight to prevent CO2 leakage Door latch Outer door Roller apparatus Roller rack fixing pin Water pan -Heated by 6 sides direct heating for humidity Outer door heater -prevent condensation CO2 inlet Safety switch Power cord Fuse holder NOTE 1. The figure 1 is to show you the name of each part of exterior incubator. 2. The diameter of blue PVC tube that we use for inflow of gas is 6mm. (total diameter 6mm, hole 4mm) Connecting Power Cord: 1. Verify your supply voltage matches the voltage of your incubator. 2. Insert the power cord into its receptacle. 3. Plug the cord into power supply outlet. 8 4. FEATURES AND SPECIFICATIONS OF THE UNIT 4.1 Features 1. Excellent Uniformity of Temperature, CO2. 2. 6 Side Direct Heat for Temperature Uniformity and Fast Recovery 3. IR CO₂Sensor detects precise density of CO₂ 4. Outer Heated Door ensures no condensation on glass door. 5. Microprocessor PID control for Temperature, CO₂ 6. Natural Humidification System by water tray and circulation fan. 7. Simple operation for Roller apparatus. 4.2 Specifications Items NB-203QR Temperature Range Ambient +5℃ to 60℃ Accuracy ±0.25℃ at 37℃ Controller Microprocessor Digital PID Humidity 70% ~ 80% at 37℃ CO2 Range 0% to 20% Accuracy ±0.1% at 5% at 37℃ CO2 Increment 0.1% CO2 Sensor Roller IR CO2 Sensor 2 stairs Roller apparatus RPM 2 rpm to 30 rpm Accuracy ±0.1 rpm Increment 0.1 rpm Time Range Continuous or up to 99h 59min Outer door Silicon Packing Magnet Door Inner door Tempered Safety Glass Door Control panel display Jacket type LED Display Dry wall type (6 sides direct heating type) Chamber material Stainless Steel (304) Weight 89kg Capacity / Shelve 179Liter / 1 shelf Chamber dimension 473x528x710(H)mm Overall dimension 560x640x940(H)mm Power 110/220V, 50/60Hz 9 5. CONTROL PANEL 1 2 3 18 4 17 5 16 15 6 14 7 13 12 8 11 1. Alarm ON/OFF switch 2. Safety Activation Indication Lamp: If Safety is activated, it will be ON. 3. Heating Signal : To show status of heating activation at 3 parts. Glass door means the heater around glass door. 4. Temperature display LED window 5. CO₂ Gas % display window 6. Pilot Lamp for CO2 valve: Pilot lamp ON position means inflow of gas 7. Roller RPM display 8. Roller operating pilot lamp (ON running, OFF not running) 9. Roller power lamp 10. Start/Stop button 11. Adjustment button for roller RPM/TIME 12. SET button for roller RPM/TIME 13. Mode key : RPM/TIME selection button 14. Calibration setting button 15. CO₂ Gas setting button 16. Adjustment button for Temperature and CO₂ 17. Temperature setting button 18. Alarm mute button 10 10 9 6. OPERATION * Before Switch ON, insert water tray filling with distilled water. Make sure connection of gas supply. And Open CO2 gas cylinder or supply with the pressure of regulator set to 4.5Psi or 0.3 bar. * Please check connection of gas supply. And Open CO2 gas cylinder for supply with the pressure of regulator set to a certain level. Co2 incubator set-up process 6-1. Place and install the product. Install the product at the desired place and check the level in all directions (side by side, front to back and ground). 6-2. Connect the power Prior to connect the power plug, make sure that the POWER S/W is off 6-3. Connect the CO2 gas ▶ Check whether CO2 gas is leaking at any point of regulator. If leak is found, please take a measure to stop leaking before supply of CO2 gas to incubator. ▶ Clear the air passage for gas input gasket at the rear of the unit. Also check the gas tube and get rid of any obstacles for smooth gas flow. ▶ Before supply of CO2 gas to incubator by gas tube, check the remaining gas volume in CO2 Gas cylinder. 11 ▶ When previous stage is cleared, connect the gas tube to regulator and incubator. At this point, Make sure that valves of all the part besides gas cylinder, Regulator are locked. (④ and ⑤ have the opposite lock direction each other. ④ is clockwise and ⑤ is counterclockwise) ▶ Open #5(Master valve of cylinder) and #4(the regulator valve), #3 Flow meter. While Flow meter fully open, do adjust regulator valve at 0.3bar. Note Once power is on with all connection for CO2 gas done, please check the solenoid valve works well by solenoid sounding. Also, observe the CO2 density on display goes up well. Factory setup for initial CO2 gas supply is up to 3%. This will be covered more at page 16. 6-4. Power switch on Once power is on, digital readout of current temperature and CO2 % in chamber will be displayed after below message passed. 6-5. Setting temperature a. Connect the power, then the LED screen will display the temperature in the chamber. b. Press the "TEMP/SET" key, then, the LED screen will flicker and display. c. Then, input the desired temperature by pushing UP (▲) and DOWN (▼). d. Press "TEMP/SET" key again after putting the desired value. “SAVE” is shown up on the LED screen as below. Note If you don't press "SET" key lastly after set-up, the new set-up value will not be saved at all. Set-up Temperature range is Ambient -8℃ (lowest 15’C at 24’C RT ~ 45℃) 6-6. Setting Up CO2 a. Press "CO2/SET" key. Then, LED screen will flicker continually. b. Input the desired value of Co2 density by pushing UP (▲) and DOWN (▼) key c. Press "SET" key again after input. “SAVE” is shown up on LED screen as below. Note If you don't press "CO2/SET" key again after set-up, the new set-up value will not be saved at all. 12 6-7 Calibration for temperature and CO2 Please follow up below procedure for calibration in case of discrepancy between actual value (measured by reliable measurement device) in chamber and displayed value. Measure CO2 density and Temperature after incubator is stabilized in which takes about more than 2 hours (you might want to perform this stabilization process at night before home) Please note that low deviation range such as ±0.1 ~ 0.3% may not be corrected precisely by this calibration. No. DISPLAY FUNCTION 1 Chamber Temperature 2 Door Heater Temperature 3 Glass Door Heater Temperature 4 CO2 Calibration 5 6 7 Heating control CO2 gas supply control To apply a new value a. Press and hold “CAL/SET” for 10 seconds. Then, LED will be flickering as below. Channel 1 is at chamber’s Main Temp calibration stage. Press UP (▲) as much as difference from measured value by precise analyzer if it is higher. Press DOWN (▼) as much as difference from measured value by precise analyzer if it is lower. Ex) If measured temperature is 38℃ and Display shows 37℃, then press up 1℃. Note * Calibration range for temperature is ±5℃ * To go to next channel is to press “CAL/SET” button. After 5th channel, the LED is back to temperature display. 13 b. Second Click “CAL/SET” ☞ Outer door’s Temp calibration Channel 2 is purposed to remove water condensing on glass door caused by high temperature difference between chamber and outside. Recommend to use calibration at Channel 2 in case of water condensing on glass door. Note Except water condensing on glass door, calibration of channel 2 and 3 is not recommendable. Check if the water condensing is removed in 3 Hours after calibration of CH2 is done. c. Third Click “CAL/SET” ☞ Door Frame Heater calibration Channel 3 is purposed to remove excessive water condensing on glass door caused by high temperature difference between chamber and outside. When failed to remove water condensing by calibrating CH2, try to calibrate d. Fourth Click “CAL/SET” ☞ CO2 density calibration Channel 4 is at CO2 density calibration stage. Press UP (▲) as much as difference from measured value by precise analyzer if it is higher. Press DOWN (▼) as much as difference from measured value by precise analyzer if it is lower. Ex) If measured CO2 value is 5% and Display shows 4%, then press up 1%. e. Fifth Click "CAL/SET" ☞ Heating control * This is pre-programmed mode before releasing from manufacturer. Push UP (▲) and DOWN (▼) to set the value. 14 NOTE Channel 5 is to set heating control point. If it is set at 3, then the heating control works from 34’C against setting value i.e. 37’C. This is in order to minimize overshooting and faster reaching time to setting value. Therefore, user is kindly required not to change this value. f. Sixth Click "CAL/SET" ☞ CO2 gas supply control. * This is pre-programmed mode before releasing from manufacturer. Push UP (▲) and DOWN (▼) to set the value NOTE Channel 6 is to set starting point of solenoid control for CO2 supply. Factory pre-programmed set point is at 2 (2 for NB203XL, 3 for NB203) to optimize set-up at 5% and it means that CO₂control valve is in open position until the CO₂density reaches to 3%. From 3% of CO2 to setting value, solenoid valve takes control of CO₂supply until it gets to setting value. When above stage is cleared, please press the button to save the new value. 6-8 Alarm In order to activate alarm system, it should maintain ±1℃, ± 1% to set point for 3 minutes. This course is recognised as stabilizing process. After stabilizing process, alarm activation is as below. Alarm Activation Temperature (beep beep beep ---) If it stays out of ±1℃ from set point for 8~9 minutes, it will give you an alarm. CO2 : (beep -------) If it stays out of ± 1% from set point for 8~9 minutes, it will give you an alarm. Door Open : Alarm is on 1 minute after door opening. No alarm if closed within 1 minute. After 1 minute of door open with alarming, -- Alarm will stop 2.5 seconds after door closed. -- Mute button will give no alarm. But alarm will activate every 10 minutes of mute button. 15 7. SAFETY SWITCH It is the safety device to prevent the heater from overheating when the temperature controller is malfunctioning. ■ Set the Safety S/W higher than setting point. ■The Safety S/W has wide deviation ■ Safety S/W is the safety device for preventing the heater to overheat when TEMP. CONTROL is malfunctioning. 8. ROLLER OPERATION 8.1 Connecting the Roller After cleaning the surface of chamber or separating the roller, please connect the roller with the shaft correctly. Axis of rotation Connected with Motor Shaft of roller Correct connection 8.2 RPM & TIME Set up RPM setting range is from 2~30rpm, and time range is from 00.00 ~ 99hours 59minutes. RPM setting change can be done by UP(∧), DOWN(∨) button and possible to change setting value during running. When the roller stops by power failure or is turned off and on by user’s mistake, then RPM shows by a message of n _ _ _ on display. (RPM is based on the number of roller rotating.) <RPM setting> ① Press RPM/TIME button to have RPM mode on display (r _ _ _) ② Press SET button for display of (r _ _ _) flickering. ③ Press UP(∧), DOWN(∨) button to change the RPM value. ④ Press SET button for saving it then rSAVE and RPM value message will appear. ⑤ Then press START/STOP button to run it. User can change RPM value during its operation by UP(∧), DOWN(∨) button and with no saving a new value. 16 <TIME setting> ① Please STOP the product first. ② Press RPM/TIME button and get TIME MODE DISPLAY( t 00.00 ). ③ Press SET button then first 2 digits of DISPLAY TIMER will be flickering. ④ Set the desired time of hour by UP(∧), DOWN(∨) button. ⑤ Press SET button once more, then last 2 digits of DISPLAY TIMER will be flickering. ⑥ Set the desired time of minute by UP(∧), DOWN(∨) button. ⑦ Press SET button to save then t SAVE on display will appear. ⑧ Start operation by START/STOP button and it will stop after set time is over with END message on display. 9. HOW TO DETACH THE ROLLER Note : Make sure to turn off the product first prior to disconnect the roller from the product. 17 10.TROUBLESHOOTING Fault description Possible cause Recommended fix Heating Chamber heating permanently, SSR relay defective set point not held Control panel display defective Temperature sensor defective Chamber does not heat up. SSR relay defective Unit does not switch on (main switch is in position “l” Replace SSR relay Replace display screen Replace temperature sensor Replace SSR relay Power not supplied to heating Reconnect the power plug on circuit the panel. Contact N-BIOTEK service The miniature fuse has blown. Replace the fuse with type 5x20mm, 220V(5A),110V(7A). If the newly inserted fuse triggers again, there is short circuit: contact N-BIOTEK service. Switch defective Replace the switch Gas CO2 concentration in chamber Defective function of the CO2 Reset the alarm. is too high/ too low. controller CO2 sensor system defective Contact N-BIOTEK service. The concentration of CO2 or Gas inlet defective Replace the gas tube O2 does not reach the Gas leaking from inner tube Replace the inner tube. adjusted set value. connecting region. Solenoid valve defective. Replace solenoid valve Humidity Condensations inside the Fan defective Replace the fan. chamber (contact N-BIOTEK service) Condensation on the door Improper temperature Increase the value of door distribution b/w the door and heating temperature. (Ch.2) the chamber No or too low humidity inside Water pan empty Fill the water pan with distilled, sterile water. Main board defective possibly Replace main board Roller Not running roller Not proper engaged with axis Make sure to fix both axis and and shaft shaft in. Manufactured By : N-BIOTEK, Inc. 402-803 Techno-park, 655 Pyeongcheon-ro, Bucheon si Gyeonggi Province, Korea Tel : +82 321 2100 E-mail : [email protected] 18
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Key Features
- Precise Temp & CO2 Control
- Natural Humidification
- Heated Outer Door
- Roller Apparatus
- Spacious Chamber
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Frequently Answers and Questions
How do I set the desired temperature and CO2 levels?
Press the "TEMP/SET" key to adjust temperature and "CO2/SET" to adjust CO2 density. Use the UP (▲) and DOWN (▼) buttons to input values, and press "SET" to save the settings.
What is the purpose of the roller apparatus?
The roller system gently rotates the culture vessels to ensure uniform cell growth and aeration.
How do I calibrate the temperature and CO2 readings?
Press and hold the "CAL/SET" button for 10 seconds to enter calibration mode. Use the UP (▲) and DOWN (▼) buttons to adjust the values for each channel (temperature, door heater, CO2). Press "CAL/SET" to save the new values.