Zeiss Universal transmitted-light research microscope Operating instructions

Large,
transmitted - light
research microscope
Universal
Operating instructions
G41-140/1-e
Table of contents
Page
lnivarcal R miernernna 4
Universal microscope 4
1. Assembly 5
1.1 In-base 6 V 15 W illuminator 5
1.2 Mounting the microscope illuminator 60 or 100 6
1.3 Fitting 32 mm dia. filters in filter holder 7
1.4 Clamping condenser carrier on change slide 8
1.5 Fitting condenser in the condenser carrier 8
1.6 Clamping specimen stage on change slide 9
1.7 Coarse and fine focusing controls 9
1.8 Mounting the tube head 10
1.9 Fitting the objective turret 10
1.10 Mounting the binocular tube 10
2. Microscope magnification 11
3. Microscope adjustment 12
3.1 Brightfield (H) 12
3.2 Phase contrast (Ph) 14
3.3 Differential interference contrast (DIC) 16
3.4 Darkfield (D) 20
4. Specimen stages 21
4.1 Mechanical stage 30x75 mm 21
4.3 Rotary centerable gliding stage 22
4.4 Circular, rotary, centerable mechanical stage 23
4.5 Scanning stages 0.5 pm and 10 pm 24
5. Special illuminator 25
6. Further operating instructions 26
Universal, transmitted-light
microscope
Fig. 1: Transmitted-light microscope Universal R
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Pushrod pushed in: all light relayed to the binocular tube
Pushrod pulled out: all light relayed upwards, e.g. to a photomicrographic
camera
Receptacle for image receivers, e.g. camera or photometer head.
When not in use close it dust and moisture-proof with a lid.
Binocular tube (473012)*
Wide-angle eyepiece
Focusing wide-angle eyepiece
Fill plug to close receptacle for analyzer slide
Slot accepting compensators or auxiliary objects
Exchangeable objective turret |
Mechanical stage with specimen holder and deep-lying controls
Specimen
Condenser
Condenser centering screws
Swing-in holder for 32 mm dia. filters
Swung-in auxiliary lens produces an image of the lamp field
stop at infinity. |
Knob for condenser adjustment.
In case of accidental lowering of the condenser carrier,
tighten the screw of this knob.
Ring for lamp field stop adjustment.
Within the ring: dust cover and filter support.
Coaxial controls for specimen movement
Coarse and fine focusing controls™*
In-base 6 V, 15 W illuminator
Fig. 2: Transmitted-light microscope Universal
1
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Pushrod in click-stop position:
white ring: ali light relayed to the binocular tube
red ring: 1/3 of the light to the binocular tube and 2/3 upwards,
e.g. to a camera
colorless ring: all light relayed upwards, e.g. to a camera
black ring: without function
Optovar magnification changer with Bertrand lens
The upper knurled ring is for focusing of the objective exit pupil where,
for instance, an image of the aperture diaphragm is formed.
Large mechanical stage with specimen holder, range of motion 50x75 mm
Microscope illuminator 60
Filter selectors
Pushbutton-operated filters in the holder are brought into the light path.
Pushing the black key removes the filter from the light path.
* The 6 or 10-digit numbers in brackets are catalog numbers which are also
imprinted on the individual modules.
** Before operating the coarse and fine focusing controls (1.18 = Fig. 1, item 18)
remove the transport lock (plastic plate under the rack-and-pinion box) by lifting
the box with the coarse focusing control.
1. Assembly
1.1 In-base 6 V, 15 W illuminator
Press 6 V, 15 W filament lamp
(3800 18-1740) (3.1) into socket
(46 8010-9903) (3.2); the red dot must
be opposite the red pin (3.3). Turn the
lamp clockwise as far as it will go and
on the bulb. o
Insert lamp with socket (4.3) in the
collector tube with lamp condenser
(46 7050) (4.1); adjust red dot on
clamping ring (4.2) to red dot on
collector tube. Secure the inserted lamp
transformer, e.g. (3925 24), connect
lamp cable (3.4) to the mains.
Adjust brightness with the voltage
generally sufficient to run the lamp at
T4 i
only for short periods.
1.2 Mounting the microscope
illuminator 60 or 100
The in-base 6 V, 15 W illuminator can
be replaced by the microscope illumi-
nator 60 or 100. Lay the microscope
stand on the left side (see Fig. 8),
unscrew the 4 screws and take off the
plate (6.1).
Unscrew screw (5.2) and remove
collector tube (46 70 50) (5.1).
Fit connection tube (46 7040-9903)
(5.3) and secure with screw (8.2). Refit
bottom plate and secure it with 4 screws.
With the supplied Allen key (6.2) swing
diffusion disk out of the light path while
the lamp is adjusted.
Fit microscope illuminator 60 or 100 to
the connection tube (7.1): loosen
clamping screw (7.3), push back its
spring bolt with the dovetails (7.2) and
let the dovetails engage.
Fa
also operating instructions G 41-305 and
G 41-310.
1.3 Fitting 32 mm dia. filters
in filter holder
Lay Universal (not Universal R) stand on
the left side (see Fig. 8).
Remove 4 screws (8.1) and take off
plate (6.1).
Push key (8.2) of selected filter holder.
Place filter (9.1) in holder (9.2) and
secure with snap ring (9.3).
The filter selectors are color-coded. It is
recommended to write down filter
positions once fixed.
Example of a filter setup from front
to rear: |
Neutral density filter 0.03 (467842)
Neutral density filter 0.12 (467841)
Neutral density filter 0.5 (467840)
Neutral density filter 0.5
Green filter VG 9 (46 7805)
Blue conversion filter
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and secure it with 4 screws. |
Filter combinations are brought into the
light path by pushing several filter
selectors at a time.
With the neutral density filters the
transmittance can be graded at a ratio
of 2:1. The transmittance of a filter set is
determined by multiplying the individual
filter values, e.g. 0.5x0.5 = 0.25.
To swing the filters out of the light path
push the black key (8.3).
on change slide (10.3)
Flick up lever (10.2), and place the right-
hand guide rail (10.4) against the
change slide. Then swing in the left side
until the spring bolt engages.
Flick the lever in horizontal position.
Lower condenser carrier as far as it will
go. Flick down lever and tighten slightly.
1.5 Mounting the condenser
Rack down condenser carrier with
control (1.15).
Press dovetails (11.1) of the condenser
against the spring bolt (10.5) of the
condenser carrier until the condenser
snaps into position. If the condenser is
provided with an orientation notch, this
must engage the spring bolt.
With control (1.15) rack up the con-
denser as far as it will go.
1.6 Clamping specimen stage on
stage carrier on change slide
With coarse focusing control (1.18) rack
down change slide. Flick up lever of
stage carrier. Place stage carrier on
change slide from above; insert at first
the lower right projection, then the left
spring bolt and finally the upper right
projection, thereby pressing the stage
carrier against the change slide. Mount
the stage carrier on the condenser
carrier, and flick down the lewer.
1.7 Coarse and fine focusing controls
act on the specimen stage.
The coarse motion can be adjusted as
follows: with the supplied
Allen key turn the hex socket-head
screw (13.2), not visible in the picture,
in the direction of the arrow to stiffen the
coarse focusing motion.
Adjusting the fine focusing control to a
medium working range: turn the fine
focusing control unit dot (13.1) is
bracketed by the two lines. Then focus the
specimen with the coarse focusing con-
trol; this leaves sufficient space above
and below for fine focusing.
One interval of the graduation (13.3)
corresponds to a vertical movement of
the stage of 2 pm = 0.002 mm.
10
1.8 Mounting the tube head on the
Universal stand
(the Universal R is supplied with rigidly
mounted tube head).
Hold the tube head (14.1) against the
dovetails of the stand (14.2), let it snap
into position and secure with clamping
screw (14.3).
1.9 Mounting the objective turret
with objectives fitted in rising sequence:
insert the turret from behind left in the
dovetails (16.1), let it snap into position
and secure it with clamping screw
(162).
Insert holder for single objectives from
the front right in the dovetails.
1.10 Mounting the binocular inclined
tube
Remove dust cover and loosen
clamping screw (17.1). With the sloped
surface down (dovetails) press down the
spring bolt underneath the clamping
screw and fit the dovetails completely.
Tighten clamping screw before letting go
the tube.
2. Microscope magnification
11
Objective magnification X factor of wide-field system (0.63) (in wide-field turret)
x Optovar factor (e.g. 1.25) X eyepiece magnification
Example: 200 = 16X0.63 x 1.25X16
2.1 Magnification table
Eyepiece 10X or 16X and wide-field system 0.63X
Objective Optovar (2.2)
1.25X 1.6 2X
Total factor Total factor Total factor
12.5% 16X 20X
‘Red dots
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1 12.5X 16X 20X
2.5 32X 40X BOX
4 50X 63X 80X
6.3 80X 100X 125X
10 125X 160X 200X
16 200X 250X 320X
25 320X 400X 500%
40 500% 630X 800X
63 800x 1000X 1250X
100 1250X 1600X 2000X
3. Microscope adjustment 12
19
> 1 Brightfield (H) (19.11), move the specimen holder
. Connect microscope illuminator with towards the specimen slide, and
transformer and the latter with the re-tighten the clamping screw.
mains, and switch on. The voltage 3. Turn in 10X or 16X objective.
selector setting should not be higher
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2. Mount the specimen in the specimen be adjusted to red ring.
holder: loosen clamping screw
5. Insert eyepieces of the same
magnification in the binocular tube,
e.g. a focusing eyepiece (19.1) in the
10. Adjust the image contrast with the
condenser aperture. To check this
stop without eyepiece or in
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the right one.
Adjust the distance between the two
tubes until a circular, sharply defined
field of view is visible.
. Adjust uniform illumination of the
objective exit pupil (Fig. 23): set the
Optovar magnification changer
(19.5) to position EY), observe the
image, and focus with the upper
the instrument IS not equipped with an
Optovar, mount centering telescope
(464822) in one tube of the binocular
body instead of the eyepiece; the
objective pupil will be enlarged (or of
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the pupil image in the centering
telescope (25.1) by shifting the
eyelens (25.2).
Centering the lamp coil in the pupil
center: loosen lamp socket (4.3) with
clamping ring (4.2), and shift the lamp
socket in longitudinal direction until the
pupil image is uniformly illuminated by
the lamp. Clamp the lamp socket.
Replace the centering telescope
again by an eyepiece, or set Optovar
. Focusing the specimen: look through
the right eyepiece and focus the
specimen with the focusing control
eye by turning the eyelens of the eye-
piece (19.1).
. Close the lamp field stop (19.10)
while observing it through the tube.
denser until the stop is in focus in the
specimen image (Fig. 20).
. Center the lamp field stop with the
screws (19.7) (Fig. 21).
Open the lamp field stop almost to the
edge of the field of view, make
precision centering, and open it
further until it just disappears behind
the edge of the field of view (Fig. 22).
objective exit pupil; the stop diameter
should cover about 2/3 of the objec-
tive aperture (23.1); see para. 6.
above.
. The use of oil immersions
With condenser apertures higher
than 0.9 and for high illuminating
apertures immersion of the condens-
er 5 recommended. With the oiler
objective apply one drop of immer-
sion oil each to the bottom surface
of the specimen slide and the con-
denser front lens. There must always
be immersion oil between an oil
glass. Turn the objective out of the
light path and apply oil to the
coverglass.
Push the resilient mount of the
objective front lens in and lock it by
turning it clockwise. Turn in an
immersion objective and immerse its
front lens in the oil. Focus with the
fine focusing control.
12. Low-power objectives (6.3X and less)
image large fields. To completely
or unscrew the condenser front lens
(19.12). Better illumination can be
achieved бу slightly lowering the con-
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(19.10) which acts here as aperture
contrast diaphragm.
The Planachromat 1.0/0.04 (46 20 10)
produces images of large object
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auxiliary lens (1.14) remains fitted.
This objective is not parfocalized
with other objectives.
3.2 Phase contrast (Ph)
14
| objective turret, and turn 10X or 16X
objective in the light path.
Insert Ph condenser (24.2) in the
condenser carrier so that the spring
bolt engages the notch of the con-
denser adapter. Set knurled ring
(24.3) to (+).
. Adjust the specimen in brightfield
(see 3.1 above).
. Swing green filter in filter holder
(24.4), in filter selector (2.5) or on
filter support (1.16) into light path.
. Turn knurled ring (24.3) of Ph con-
denser to a position which cor-
responds to the Ph objective, e.g. to
(2) for a Neofluar 16/0.40 Ph.
. Replace one eyepiece by the
centering telescope (464822) (25.1)
and shift the latter's eyelens, or set the
phase ring and annular diaphragm
(Fig. 26).
. With lockabie lever (24.5) and knob
(24.6) of the Ph condenser move the
bright ring to lie within the black one
(Fig. 27).
. Replace the centering telescope
(25.1) by an eyepiece or set the
Optovar to a magnification system
Through the binocular tube you will
see the Ph image.
After objective exchange always
adjust the lamp field stop (1.16) to
the size of the field of view (see 3.1.9
above), and with the knurled ring of
the condenser turn in a Ph annular
diaphragm which corresponds to the
Ph objective (see 3.2.4 above). Check
the centering of the annular dia-
phragm after specimen exchange.
Specimen preparation for phase-
contrast work
For satisfactory results the microscopic
specimens must be optimally adapted to
the optical conditions of the phase-
contrast method. The high sensitivity of
the method requires particularly careful
preparation. Use only optically perfect
(schlieren and bubble-free) specimen
slides and coverglasses, and always
remove all residues of detergents on
specimen slides and coverglasses. The
specimens must be limited by plane
surfaces, and the examination of
specimens in suspended drops or even
in hollow-edged specimen slides is not
possible. For this kind of work we
recommend an oil chamber.
Some necessary plastic rings are
supplied with the mounting media
(4629 29). This chamber has proved
particularly valuable for the observation
of live specimens.
Oil chamber
a = paraffin о!
b= 0.5 - 1 mm thick plastic ring
c = culture medium
d = coverglass
e = specimen slide
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This technique produces a visible relief 3. With the lamp switched on and the
of differences in the optical path length lamp field stop open, look through the
of unstained specimens (product of empty tube.
mechanical height and refractive index). Slide in analyzer (29.1) as far as it
The contrast also depends on the orien- will go.
tation of a structure in the field of view Set scale of rotating analyzer
should therefore be rotated around the must be achieved in this position. Re-
optical axis, preferably on a rotary correct in case of slight deviations.
microscope stage. Retain the position of maximum
extinction for DIC examinations. Swing
3.3.1 Orientation of polarizer and in auxiliary lens (29.8) and insert
analyzer eyepiece in tube.
1. Remove condenser, auxiliary lens,
objective, DIC slide and eyepiece
from the light path.
2. Swing in the polarizer and adjust its
oscillation direction East-West: with
lever (29.16) swing polarizer
(47 08 65) into light path, and set the
corresponding scale to 0° with lever
(29.7) or put 32 mm dia. polarizing
filter (47 36 00) in filter holder (1.13) or
on filter support (29.10) so that both
white lines on the filter mount are in
East-West (right-left) direction.
1/
3.3.2 Fitting the DIC equipment 3.3.3 Object focusing in DIC
1. Insert DIC-Ph-H-D condenser (29.12) 1. Focus object, preferably with Plan-
(see table on p. 19) in condenser achromat 16/0.35 and corresponding
carrier (10.1) (see section 1.5 on p. 8). DIC prism in position © of the DIC
| condenser (29.14).
2. Screw objectives (see table on p. 19) Check microscope adjustment
into quintuple DIC objective turret according to section 3.1 above.
(29.2) (47 3156) which features five Center condenser aperture with lever
oriented, fixed DIC adapter rings and knob (29.15) and open it with
(29.3) (47 44 65). Replace the knurled ring (29.13) until 2/3 of the
standard objective turret by the DIC objective exit pupil are covered (see
objective turret. From the front left to para. 3.1.10 above).
the rear right slide the DIC slider
(29.4) (see table on p. 19) into the slot 2. By turning screw (29.5) of DIC slider
of the DIC adapter ring, corresponding select black-and-white contrast and
to each objective and with the adjust it to the image structures to be
engraving pointing upwards. examined.
Other objectives on the DIC turret,
e.q. for phase-contrast work, are 3. A-plate (47 37 04) (29.11) in the light
screwed into the empty DIC adapter path produces color contrast.
rings or mounted via spacer ring
(47 44 66). 4. The DIC equipment can also be used
Only then are all objectives par- - lo observe weakly stained specimens
focalized. in amplitude contrast. To achieve
Subsequent fitting of DIC adapter maximum contrast polarizer or
rings (4744 65) and alignment of the analyzer must perhaps be slightly
slots for DIC sliders see operating turned out of the cross position.
instructions G 41-215.
DIC prisms in the DIC condenser
if the condenser was not supplied with
the microscope.
Checking the prism position: take DIC
condenser out of the condenser carrier.
Remove dust cover (Fig. 31) at con-
ring until the DIC prisms are visible.
The notch marked with a line (32.1)
must be assigned to the pin (33.1) of
the turret. If the line is in opposite
direction, the DIC prism must be
tube (34.1) into the DIC prism mount,
pull out the prism and insert it again in
correct orientation. The symbols (30.1)
and (30.2) indicate the DIC prism notch
positions relative to the pin of the
condenser ring. T means that on the
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marked by a line must engage the pin.
S means that on the Standard micro-
scope the notch marked by a dot must
engage the pin.
Table
Objective DIC slider DIC condenser DIC condenser
Cat. No. aperture 0.03 aperture 1.4
(465273) (465285)
position (29.14) position (29.14)
Plan 6.3/0.16 474531 | *
Plan 16/0.35 474551 | |
RO 000 474564 I |
Plan 40/0.65 4745 71 | ||
Achromat 40/075 W 434501 || |
Planapo 63/1.40 oil 474581 | |
Plan 100/1.25 oil 474591 | |
Se 47 4555 ] |
25/08 Woil go, 174960
Neofluar 434500 | |
Plan 63/0.9 434500 | I
without coverglass
*The field of view is inadequately
illuminated.
Condenser position (J) (iris) for bright-
or Ph 3 objectives, TT
Condenser setting(ll is intended for
special prisms.
3.4 Darkfield (D)
3.4.1 The microscope equipment for
darkfield corresponds to that for
brightfield (Fig. 19, p. 12).
A darkfield condenser replaces the
brightfield condenser. The darkfield con-
denser illuminates the object with a
aperture must be higher than the
objective aperture. Only the light
refracted by the object reaches the
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3. The use of oil immersion objectives
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diaphragm. Push in the resilient
mount of the objective front lens and
lock it by turning it clockwise.
Apply immersion oil to the coverglass
without bubbles.
Immerse the objective front lens.
(35.1).
Focus the object.
Re-adjust the lamp field stop. Open
the objective iris diaphragm only so
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3.4.3 Adjustment with dry darkfield
condenser
Insert dry darkfield condenser in the
condenser carrier. Swing a dry objective
into the light path with an aperture that is
smaller than that of the condenser. Do
ments see para. 3.4.2 above.
image remains dark.
3.4.2 Object adjustment with
immersion condenser
1. Insert H-Ph-D condenser V/Z
(4652 77) or ultra condenser
(465500) (35.2) on condenser
holder Z (465542) (35.3) in the
condenser carrier. Apply a drop of
immersion oil bubble-free on the con-
denser front lens. Provide a specimen
and rack up the condenser until the
immersion oil has reached the
specimen slide.
2. Focus the object with 10X to 25X
objective. Close lamp field stop
(19.10).
Adjust the height of the condenser
until the light spot in the field of view
is small, bright and in focus. With the
centering screws (19.7) center this
image of the lamp field stop in the
field of view. Open the lamp field stop
until its image just disappears behind
the edge of the field of view.
4. Specimen stages
21
The specimen stage is fixed with 4
screws on the attachable stage carrier
(47 1540), and clamped to the change
slide of the microscope (see section 1.6
p. 9). From the ample selection of
specimen stages the user can choose
the one which best suits his purposes.
Some stages are described below.
4.1 Mechanical stage 30 X75 mm
with graduation and deep-lying controls
right (4734 15) (Fig. 1.9) or left
(4734 16). The range of motion can be
extended to 50 X75 mm by fixing the
stage with its 4 screws on the stage
carrier turned through 180° so that the
controls are in front (nearer to the user).
The outer stop screw at the bottom of the
stage must then be unscrewed.
4.2 Large mechanical stage with a
range of motion of 50 X75 mm, and
controls either left (47 34 25) or right
(47 34 26). One of the specimen holders
of the mechanical stage with graduation
is provided with index and vernier (37.1).
A specimen area can only be relocated
according to its coordinates if the
specimen holder with index and vernier
has not been moved. To hold it use only
the other specimen holder, shift it
towards the specimen slide and tighten
the clamping screw. With the graduation
a specimen area can be determined
most accurately to within 1/10 mm, and it
can always be relocated with its coordi-
nates. The values of the x (left-right) and
y (forward-backward) directions are
noninterchangeable (Fig. 37).
Fig. 37 shows the readout 90/18.5.
The spring pressure the clamp exerts on
the specimen slide can be adjusted by
pressing down knob (38.1) turning it
and letting it engage again.
raie |]
22
4.3 Rotary and centerable gliding
stage with centering piece (47 34 54)
and stage clips (473373)
The stage plate can be shifted by hand
and turned using two pins (39.2). During
stage rotation the object remains in the
field of view if the axis of rotation is
centered relative to the center of the field
of view.
Centering the gliding stage
Swing a 10X or 16X objective into the
light path. Turn and shift the gliding
stage so that a prominent feature lies on
the axis of rotation. Mark the center of
the field of view by closing the centered
lamp field stop. With socket wrenches on
the centering screws (39.1) center the
axis of rotation in the field of view. The
stage rotation can be locked with
clamping screw (39.3).
If the gliding stage was not in use for a
longer period, shift it several times in all
directions to smoothen the motion.
To maintain the gliding properties of the
stage and for correct microscopy at all
magnifications, grease the gliding
surface of the stage every six months.
Suitable oil (10 ccm) (473391) is
supplied with the equipment.
Greasing the gliding stage
Unscrew centering screws (39.1).
Press stage against spring bolt (40.1)
and take it out of the centering piece
(Fig. 40).
Separate bottom plate of stage (Fig. 41)
of the guide frame in the base plate
(42.1) and the base plate’s position with
respect to the notches of the stage plate
(Fig. 41).
Thoroughly clean all gliding surfaces
with xylol. With a finger apply an ultra-
thin film of oil to the gliding surfaces. Re-
assemble the gliding stage. The spring
bolt (40.1) must engage the notch in the
base plate which is used to press it
back.
After re-assembly shift the stage plate
several times in all directions to
distribute the oil. If the gliding motion is
too smooth you have used too much oil.
CO
43
AR LN =
mechanical stage, 50 X75 mm range
of motion, with centering piece
(473456)
Centering the stage
With a 10X or 16X objective in the light
path focus a fine-grain object by evenly
turning the specimen stage.
Turn the socket wrenches on the
centering screws (43.4) so that the
center of rotation of the stage moves to
the center of the field of view.
The center of rotation of the stage is
that point of the object which only rotates
but does not move on a circle. The
center of the field of view can be
marked by the centered, closed lamp
field stop.
The rotary motion of the stage can be
control (43.1) the object is adjusted in
X direction. |
With control (43.2) the object is adjusted
in Y direction. lts motion can be stiffened
or smoothened with screw (43.3).
44
24
45 Scanning stage for 0.5 um
(47 3481) and 10 pm increments
(47 34 82)
The stage is similar to the large mecha-
nical stage (see p. 21). With the stepping
motors (44.1) on both sides object fields
of max. 25X75 mm can be scanned.
An object feature can only be relocated
according to its coordinates if the
specimen holder with index and vernier
(44.2) has not been moved. Its outer
edge should always lie at the inner index
line of the stage. For holding press the
right specimen holder (44.3) against
the specimen slide.
Clamp the specimen in the holder. Never
press the specimen against the stage
plate from above (e.g. with clips),
because friction would hinder the
scanning motion.
The stage can be coarsely adjusted with
the handwheels on the motor housings
(44.1). They are coupled with the gear
by means of a slip friction clutch. The
scanning stage is operated via motor
control 46 (47 74 46) (see operating
instructions G 41-907).
5. Special illuminator
45
46
The special illuminator (Fig. 45) accepts
the microscope illuminator 250 (46.1) via
holder 465644-9902) (45.1) with
opening for continuous interference filter
b, and illuminator 60 (46.4) via holder
(45.5).
The special illuminator (47 20 15-9902)
can only be used with microscopes with
65 mm high bases, while the special
illuminator (472016) is intended for
microscopes with 50 mm high bases.
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A mirror system (46.2) is switched with
the pushrod (46.3).
AU ES ——
Pushrod (46.3) down:
sata Allan Lomas mmivvme FAR TN Avid Alaa Al
illuminator 250 (46.1) transmitted light;
Mirror (46.2) switched according to
drawing B:
illuminator 250 (46.1): not operative;
illuminator 60 (46.4): reflected light.
i
”
Pushrod (46.3) up:
the mirror system is not in the light path,
and upper and lower light sources have
free passage.
Lay the microscope on the left side.
Instead of the collector tube with lamp
condenser (46 7050) (5.1) mount the
tube (45.1) supplied with the special
illuminator according to section 1.2
on p. 6.
Unscrew the four rubber supports
(8.4, p. 7) of the microscope. Remove
dust cover (5.4, p. 6) on the back of the
stand.
Place microscope on blase plate.
To screw the base plate to the micro-
scope, let the latter protrude over the
table edge so that one borehole after the
other becomes accessible, screw the 4
supplied screws in and tighten them.
For more details of microscope illu-
minator 250 see operating instructions
G 42-320, of microscope illuminator 100
G 41-310/ and of microscope illumi-
nator 60 G 41-305.
6. Further operating instructions
Further equipment and accessories for
the Universal microscope see the
following operating instructions:
G 41-350 Fluorescence equipment for
transmitted-light excitation
G 41-351 Epi-fluorescence condenser
HRS
G 41-415 MC 63 photomicrographic
camera
G 41-500/1 Accessories for transmitted-
light polarizing microscopy
G 41-820/1 Microscope photometer 01 K
G 41-823/l Electronic shutter sequence
cantrol
G 41-825 Microscope photometer 03
We reserve the right to change design or
extent of instrumentation in the course of
advanced development.
These instruments may not be used in
locations with explosion hazards.
The repair of electromedical equipment
shall be the exclusive responsibility of
our repair and maintenance service.
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