Olympus IX 50, IX 70 inverted microscope Instructions

PDF
Dokument
Reklama
hh
4
A
OLYMPUS
INSTRUCTIONS
IX50/1X70
INVERTED SYSTEM MICROSCOPE
This instruction manual is for the Olympus Inverted System Microscope Models IX50 and IX70. To -
ensure the safety, obtain optimum performance, and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating
the microscope. Retain this instruction manual in an easily accessible place near the work desk
for future reference.
- AX7066
ve
`
The IX system comprises two basic microscope frames, the IX50 and the 1X70. Various modules, such as
illumination column, stages, condensers, etc. are available for upgrading the system. This instruction manual
introduces the basic system. Explanations relating to particular modules are preceded by a heading stating
the name of the module. Please study carefully the relevant sections dealing with the modules you have
already purchased.
This 1X50 and 1X70 units feature a UIS (universal infinity system) optical design, and
should be used only with UIS eyepieces, condensers, etc. Less than optimum
performance may result if inappropriate accessories are used.
Precautions When Unwrapping the Micoscope
\
1.
Before use, using the Allen wrench provided with the microscope frame,
loosen the screw (1) securing the clamping rod holding the focusing
controls clamped to protect them during transport. (Fig. 1)
2. Remove the screw and the caution tape (2) around the focusing adjust-
3.
ment knobs. Then rotate the coarse adjustment knob counterclockwise
(in the direction of the arrow), and remove the rod (3) holding the focusing
controls. (Fig. 1)
When moving the microscope, insert a thick sheet of paper, etc. into the
space left by the clamping rod between the revolving nosepiece and the
dummy cover (or cube cassette) and turn the coarse adjustment knob
clockwise to prevent the focusing block from moving.
* Never attempt to turn the coarse adjustment knob clockwise while
the rod for clamping the focus controls remains in place.
Before moving the stage, clamp the flexible knob to prevent it from being
damaged.
+ у
Co .002, 0155 pt PE OT
Ay A UN a 8
Ta EEE EE AAA E
=
a
1.
2.
= тат
es a. ERA so
Install the microscope on a sturdy, level table or bench. (Weight: 1X70
approximately 28 kg (62 lb.); X50 approximately 27 kg (59.5 16.))
The surfaces of the lamp housing (3) on the illumination column will
become extremely hot during operation. When installing the microscope,
make sure to allow ample free space around and in particular above
and below the lamp housing. (Fig. 2)
3. When moving the microscope, grip at the designated position (1) and
the handle (2) on the illumination column IX-ILL100 (the IXILL30 has a
groove instead of the handle). (Fig. 2)
* Damage to the microscope may occur if you grasp it by the lamp
housing or the binocular eyepiece section.
TT
IX50/IX70
N 4. To avoid potential shock hazards and burns, first set the main switch
@ to “O” (OFF) and then disconnect the power cord from the wall
outlet before replacing the bulb and fuses (3). Whenever you replace
the bulb during use or right after use, allow the lamp housing and bulb
to cool before touching. (Fig. 3)
Designated bulb | 1X70 (IX-ILLTOOLH)
12V, 100WHAL-L (PHILIPS 7724)
IX50 (X-ILL30)
&gt; 6V, 30WHAL (PHILIPS 5761)
Fig. 3 “| Designated fuse | IX70 (IX-ILL100LH)
= TBA (H) 250V (LITTLEFUSE 215005)
IX50 (IX-ILL30)
== 13.15A (H) 250V (LITTLEFUSE 2153.15)
Use of improper bulb or fuse could result in a fire.
5. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD&quot; at the end of
this instruction manual.
If the proper power cord 1s not used, product safety and performance
cannot be guaranteed.
6. Always ensure that the ground terminal of the microscope and that of
the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety and performance -
of the equipment.
.7. Never insert metal objects, etc. into the air vents of the microscope
frame as this could result in electrical shock and personal injury.
| Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols, and always use the equipment
in the safest possible manner.
1
Symbol Explanation
AN Indicates that the surface becomes hot, and should not be touched with bare hands.
A Before use, carefully read the instruction manual. Improper handling could result in injuly to
the user and/or damage to the equipment, _
Indicates a potential fire hazard; when replacing fuses, be sure replacement fuse is of the
specified rating.
| Indicates that the main switch is ON.
O Indicates that the main switch is OFF.
Warnings
Warning engravings are placed at parts where special precaution is required when handling and using the microscope.
Always heed the warnings.
Warning engraving position Lamp housing | Warning against high temperature |
Getting Ready
1.
2.
3.
4.
A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust, or vibrations.
(For operating conditions, refer to Section 9, SPECIFICATIONS on page 53.)
Always use the tension adjustment ring to adjust the tension of the coarse adjustment knob.
In case of the IXb0, make sure that the line voltage selector switch located at the rear of the microscope is set
to conform with the local mains voltage.
O When shipped from the factory, the switch is set to 220-240V.
ea Maintenance and Storage
1.
Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze
slightly moistened with a mixture of ether (70%) and alcohol {30%) or EE System Cleaner (Olympus EE-6310).
A Since solvents such as ether, alcohol and EE-6310 are highly flammable, they must be handled carefully. Be
sure to keep these chemicals away from open flames or potential sources of electrical sparks — for example,
electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a
well-ventilated room.
. Do not attempt to use organic solvents to clean the non-optical components of the microscope. To clean these,
use a lint-free, soft cloth lightly moistened with a diluted neutral detergent.
. When handling fluids, e.g., culture solutions, be careful not to spill these. If fluids are spilled, immediately set the
main switch to “O” (OFF) and disconnect the power cord and wipe off objectives and any fluid running below
these. Pay special attention to the dust cover glass below the revolving nosepiece and storage hole for the Allen
screwdriver on the microscope frame.
. When objectives are not screwed into the sockets of the revolving nosepiece, seal the sockets with dust caps,
which will protect the lenses located in the lower light path from dust, spilled solution, etc.
5. Do not disassemble any part of the microscope as this could result in malfunctions or reduced performance.
6. When not using the microscope, keep it covered with the provided dust cover.
7. To prevent the risk of infection, immediately wipe with disinfectant alcohol after using specimens containing infectious
material. ~
Caution
If the equipment is used in a manner not specified in this manual, the safety of the user may be imperiled. In
addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
ен =
не — = вмтитееоы о
Module System Diagram essen R RR HERR RAR RR KB R RR KERRKEURRELKGG
Detailed Assembly Procedure esse creer en
4-1 Brightfield/Phase Contrast Observation (Microscope Frame 1X70,
lumination Column 100W Configuration) ........................esemeenieras 19
4-2 Brightfield/Phase Contrast Observation (Microscope Frame IX50,
lllumination Column 30W Configuration) ....................eeseecsiircas 20
4-3 Transmitted Light Nomarski DIC Observation
(When Using the Universal Condenser IX-LWUCD) ..................... 21
5-1 Base ..............eesviecvrrvenoerrcanrerereseaasenaceeneneeeacenaceacenr reee e e 22
Focusing Adjustment Knobs ...................eevvveverricensrricenenenen 24
D-3 Stage ..........e...eessircceciccerieeoriie rene irene can e rene ee ee e 25
54 Observation Tube …..........…..…....rrrcerrenmerercenanrentenserrencansenrececns eee 28
| 5-5 lllumination Column ......енненненеенееенееенееенееенеенееееееее. 31
| 5-6 Condenser are 34
5-7 &copy; 1 &cent; T= ox (1 V/= JR 15
q
N
6-1 Differential Interference Contrast ObservatiON oo... 41
6-2 Simple Polarized Light ObservatiON vers 43
7-1 Still Photography .....................eesecesveerreciruneiecenerezan rre ere e 44
72 TV Observation..........................esiveceriiecriie ere re Dee 48
“ PROPER SELECTION OF THE POWER SUPPLY CORD....................... 56
IX50/1X70
ami MODULE NOMENCLATURE
Lamp housing
o o
to
D
0
c
D
&copy;
=
о
O
lilumination
| Observation tube
т
a
o
o
an
2 0
sa
&gt;
&copy;
&pound;35
wn
3
о с
00
uu O
7
y
/ 5
|
n 2
SE
2.
Eo x
Eo
w= D
со О
(2-1 Module System Diagram |
The diagram below shows where the various modules should be mounted.
* When assembling, make sure that all parts are free of dust and dirt, and avoid scratching any parts or
touching the glass surfaces.
* Before assembling, make sure to prepare all required tools (i.e., Allen screwdriver provided with the
microscope frame and the provided Allen wrench).
Turret inserts | IX-PHL Phase contrast
IX-PHC
on omer
IX-PH2 12V100W
enana о &Ntilde; IX-PH3 Phase contrast halogen lam
: PM system ; IX-DP10 element CU 12V100WHAL-L
: TV system | IX-DP20 IX-PHCU
E IX-DP40 | = Q
IX-DP60 ULWCD
‘ Condenser 100W transmitted
ru ographie IX-ULWCD illumination
IX-SPT aa column LH
Eyepiece uE IX-ILL100 LH
35WH10X ——
WH10X Ш о?
WH10X-H Un, Polarizer | —
IX-LWPO
Intermediate |
Q) tube 1 o
Focusing &gt;
telescope IX-ATU Va LWD —
U-CT30 Universal
condenser O
Binocular IX-LWUCD i
tube 90CT ULV
U-BISOCT UCD Adapter nell + Con
&gt; IX-ADUCD XL
Binocular
tube 90
U-BI90
Universal condenser
U-UCDB
Transmitted 2
differential Objective lens 5
interference
prism |
U-DICT
U-DICV — | _
: Lb O 77
UN
— a В
MMS-R2 в Е IX70S1F2/S8F2
Release 1 TVsystem | Microscope
PM system | и
| F-mount | |
' video camera \
DTU * FT-mount Photographic
U-FMT tube —
IX-SPT
; C-mount eve Ц |!
| video camera :
ernennen A C/T-maunt Video adapter
U-CMT IX-TVAD
IX50/IX70
Terasaki plate holder
IX-HOT
Slide glass holder
IX-HOS
Petri dish holder
IX-HOP
AlaNSSY ANO
Specimen Intermediate
sm Use ero
Lamp socket - -
U-LS30-3 A
O IEEE
Mechanical stage
30W Halogen lamp | IX-MVR
30W transmitted
9 illumination column ю
a | IX-ILL30 | | |
Ring slit stider 1 I r 1
T то, L2 A, a CU от [ y -
| Cross stage R Cross stage L Ш Plain stage Sub-stage
I | IX-SFR IX-SVL IX-SP CK2-SS
ULWCD SLWD Condenser
Condenser IX-SLWCD
IX-ULWCD
\ Objective lens 5
=)
Ne”
Y
= )
IX50-S 1F/S8F
Microscope
Er |
Tools
=== |
i
u
i
i
i Allen screwdriver
i {stored inside the
1 microscope frame)
E
i
i
i
u
a
Allen wrench
{provided with the
microscope frame)
Йона на нон о ни ша ни ни ни Ши Ш
0 _ CTT TT Er A y o TL LL A a Tal ES AE
ASSEMBLY В
i
&Ecirc;
\
Fig. 5
(3)
2)
No
Fig. 6
~
= &deg;
== с TT
To
. Ol
D
==
=
SIN
I
о о US
S
—
N
С 2-2 Detailed Assembly Procedure X
Mounting the lllumination Column
(Fig. 4)
1. Aligning the guide holes in the illumination column with the two protruding
positioning pins A) оп the microscope frame, gently place the column onto
the microscope frame from above.
While pressing down the illumination column with one hand, insert the four
provided hexagon socket head screws (2) into the screw holes. Then
tighten the screws with the provided Allen wrench (3).
Mounting the Bulb and the Lamp Housing (Figs. 5-10)
| Hllumination Column {IX-ILL100LH)
(Figs. 5-7) |
O The designated bulb is a 12V, T00WHAL-L halogen bulb (PHILIPS 7724).
1.
Using the Allen screwdriver, loosen the clamping screw (1) on the right
side of the lamp housing and detach the lamp housing. (Fig. b)
Holding the bulb with a piece of gauze to avoid touching it directly with bare
hands, depress the bulb clamping levers (2) in the direction of the arrow
and insert the pins of the bulb 3) fully into the pin holes @). (Fig. 6)
Gently release the bulb clamping levers to their original positions to secure
the bulb.
&Agrave; To prevent reduced bulb life or cracking, do not touch the buib
with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.
A Bulb Replacement During Use or Right After Use
The bulb and the lamp housing surfaces and vicinity will be
extremely hot during use and right after use. Set the main switch
10 “О” (OFF) and disconnect the power cord from the wall outlet.
Then allow the old bulb, lamp housing and vicinity to cool before
replacing the bulb with a new of the designated type.
4. Using the Allen screwdriver, loosen the clamping screw (1) on the right
hand side of the lamp housing. Mount the lamp housing horizontally and
securely tighten the clamping screw. (Fig. 5)
Align the plug &reg;) of the connecting cord extending from the illumination
column with the notch in the lamp power output connector (6) on the rear
of microscope frame and connect them firmly (Fig. 7). |
* Thelamp housing andthe areainthe vicinity of it will become very
hot during operation. Ensure thatthere is ample free space around
and in particular above the lamp housing.
_IX50/1X70
| illumination Column (IX-ILL30) (Figs. 8-10) |
&copy; The designated bulb is a 6V, 30WHAL halogen bulb (PHILIPS 5761).
1. Holding the bulb (0) with a piece of gauze to avoid touching it directly with
bare hands, insert the bulb pins 2) fully into the pin holes (3) of the lamp
socket. (Fig. 8)
A18N3SSY ENO
A To prevent reduced bulb life or cracking, do not touch the bulb
with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.
\
в 2. Insert the plug @) into the socket pin 6). Then align the guide pins (&copy; with
the guide holes (7) on the illumination column, and gently place the lamp
socket on the illumination column. {Fig. 9)
A\ Bulb Replacement During Use or Right After Use
The bulb and the lamp housing surfaces and vicinity will be
extremely hot during use and right after use. Set the main switch
to “O” (OFF) and disconnect the power cord from the wall outlet.
Then allow the old bulb, lamp housing and vicinity tn cool before
replacing the bulb with a new of the designated type.
N
) 3. Plug the connecting cord plug firmly into the lamp power output
connector (9) on the rear of the microscope frame. (Fig. 10)
* The lamp housing and the area in the vicinity of it will become very
hot during operation. Ensure that there is ample free space around
and in particular above the lamp housing.
ITI
Fig. 10
ASSEMBLY NRO
Mounting the Stage (Figs. 11-18)
&copy; The stage mounting procedures are the same for the stages IX-SFR,
IX-SVL, and IX-SP.
&copy; When mounting the mechanical stage (\X-MVR), first attach it to the plain
stage (IX-SP). (See page 7.)
1. Aligning the stage mounting holes with the threaded holes in the micro-
scope frame, gently place the stage on the frame.
&copy; Position the closely spaced stage mounting holes at the front.
2. Insertthe four provided Allen screws into the mounting holes (0). Tighten
the screws with the provided Allen wrench. (Fig. 11)
3. Insert the stage insert plate @ into the stage. (Fig. 12)
x The stage insert plate is designed very thin so that the objective
will not hit it when the revolving nosepiece is rotated. Do not
subject the stage insert plate to impact or excessive force, as this
may deform it.
O To facilitate confirmation of the objective front lens position and applica-
tion of oil for oil immersion objectives, rotate the stage insert plate so that
the keyhole (&reg; will be facing the front. (Fig. 12)
4. Screw the stage clips (U-SCL) (9) into the threaded holes provided on the
stage surface. (Fig. 13)
| Attachable Mechanical Stage (IX-MVR) (Figs. 14-16) |
{ Mounting the Mechanical Stage Y
1. Invert the mechanical stage 4). Invert the plain stage (IX-SP) (2) and place
it on top of the inverted mechanical stage. (Fig. 14)
2. Align the two mounting holes 3) on the mechanical stage with the
mounting holes @) on the plain stage. Insert the two clamping screws (5)
and tighten with the Allen screwdriver.
* The mechanical stage may also be attached to the left side of the
plain stage. However, this will interfere with the operation of the
stage travel knobs, focusing knobs, and the revolving nosepiece.
Always attach the mechanical stage on the right side of the plain
stage.
IX50/1X70
) { Attaching the Scales) 2
&copy; Scales for use with a 96-well microtiter plate are provided with the
attachable mechanical stage.
1. Loosen the two clamping knobs (&cent;) at the top of the stage’s X-axis guide.
Positioning the scale numerals correctly, place the X-axis scale (7) on the
guide. Then tighten the clamping knobs. (Fig. 15)
AT9WN3SSV
2. Positioning the scale numerals correctly, place the Y-axis scale (8) on the
top of the stage’s Y-axis guide. The scale will be held in place magneti-
cally.
&gt; ( Mounting the Stage Extender Plates (CKS2-SS) )
Mount these plates in the same manner as outlined in “Attachable
Mechanical Stage (IX-MVR)” above. (See page 6)
&copy; When the mechanical stage is not used, a stage extender plate (9) can
be mounted at both sides of the plain stage. (Fig. 16)
{ Mounting the Positioning Bracket Y
&laquo; &copy; Flexible stalks for the coaxial X-Y movement knobs are employed in
the case of the stage (IX-SFR) and the attachable mechanical stage
(IX-MVR). These stages are provided with a positioning bracket that
allows the tilt of the stalk to be adjusted to suit the observer's
| preference.
| Stage (IX-SFR) (Figs. 17,18) |
1. Insert the stalk 2) of the flexible X-Y movement knobs into the U-shaped
notch in the positioning bracket 0). (Fig. 17)
2. Using the Allen screwdriver, tighten the clamping screw 3) of the bracket
lightly to the stage.
N
Fig. 17 3. Alignthe stage's center alignment index line (4) with the edge of the upper
stage (8). (Fig. 18)
\ 4. Slide the positioning bracket to and fro in order to determine the most
$ convenient stalk angle.
5. Tighten the clamping screw (3).
4)
| Attachable Mechanical Stage (IX-MVR) |
e |sattachedinthe same manner asoutlinedin “Stage (IX-SFR)&quot; above. The
attachablemechanical stage, however, 1s not provided with a stage center
alignment index line. Instead turn the Y-axis travel knob to bring the stage
N
into the center.
ASSEMBLY 5
‘Mounting the Objectives I (Figs. 19, 20)
1. Turn the coarse adjustment knob (1) in the direction of the arrow to lower
the revolving nosepiece. (Fig. 19)
2. Remove the stage insert plate and mount the objectives through the
hole in the stage left by the stage insert plate.
&copy; Mount the objectives onthe revolving nosepiece in such a manner that the
magnification increases from low to higher powers in a clockwise direc-
tion.
* In the inverted microscope, the front lens of the objective faces
upward, and is more exposed to contamination than the objective
of an upright microscope. Therefore, if there are empty sockets in
the nosepiece, attach the dust caps 2) provided. (Fig. 20)
Mounting the Condenser Turret Inserts (Figs. 21-28)
O The turret insert centering wrenches (1) provided with the condenser may
be conveniently stored in the storage holes provided on the microscope
frame. (Fig. 21)
| Universal Condenser (IX-LWUCD) (Figs. 22 — 26) |
&copy; See page 51, “TURRET INSERTS AND COMPATIBLE OBJECTIVES”, for
details on the various combinations.
&laquo; Mounting the Phase Contrast Ring Slit Insert )
1. Position the condenser as shown in Fig. 22. Loosen the two clamping
screws (2) and remove the cover (3).
2. Rotate the turret so that the number of the insert that is to be mounted
becomes visible in the opening.
3. Put the turret insert centering wrenches @) into the designated insert
centering holes and turn the wrenches counterclockwise. (Fig. 22)
&copy; Seepage 51, &quot;TURRET INSERTS AND COMPATIBLE OBJECTIVES” for
turret opening diameters and compatible turret inserts.
IX50/1X70
&raquo; 4. While depressing the spring (6) on the inward side of the turret aperture
with the side of the phase contrast ring slit mount (6), insert the phase
&copy; contrast ring slit completely until it sits firmly on the bottom of the turret
aperture. (Fig. 23)
* Be careful not to press against the ring slit glass plate in its mount.
ATaNISSY В
5. Turn the turret insert centering wrenches clockwise to tighten lightly.
* If the centering wrenches are engaged, the turret cannot be rotated.
* Do not tighten the centering wrenches excessively as this may
deform the insert mount.
N 6. Attach the index sticker (7) which comes with the insert at the index
number (9) position that corresponds to the number (8) into which the
turret insert was placed. (Figs. 24, 25)
O The turret insert corresponding to the number indicated by the * symbol
is engaged into the light path. (Fig. 25)
&copy; Remove the turret insert index sticker with the tip of a bal-pen or mechanical
pencil.
7. When all the turret inserts have been mounted, mount the cover and
tighten the two clamping screws.
{ Mounting the Differential Interference Contrast Prism Insert Y
—
1. Follow steps 1, 2, and 3 in the above paragraph.
2. Aligning the positioning dot (1) of the DIC prism insert with the positioning
dot (2 of the turret aperture, drop the DIC prism insert into the turret
aperture as far as it will go in a manner so that the positioning pin of the
DIC prism insert fits into the pin hole. Slightly depress the spring (3 on
the inward side of the turret aperture with the side of the DIC prism insert
mount when inserting. (Fig. 26)
Xx Be careful not to touch the prism in its mount.
_ * Do not tighten the centering wrenches excessively as this may
Fig. 26 deform the prism mount.
3. Follow steps 5, 6, and 7 in the above paragraph.
ASSEMBLY JANG)
10
| Condenser (IX-ULWCD) (Figs. 27, 28) |
{ Mounting the Phase Contrast Ring Slit Insert ))
1. Rotate the turret (OD to the Ph1/PhC index position. (Fig. 27)
2. Put the turret insert centering wrenches &copy; into the designated insert
centering holes indicated by the 4 symbols 3 . Turn the wrenches
counterclockwise to loosen.
3. Rotate the turret to have the BF index appear in the front window.
4. Loosen the two clamping screws @) and remove the cover (5).
5. While depressing the spring (6) on the inward side of the turret aperture
with the side of the phase contrast ring slit mount (IX-PH1U or IX-PHCU
(7), insert the phase contrast ring slit completely until it sits firmly on the
bottom of the turret aperture. (Fig. 28)
* Be careful notto press against the ring slit glass plate in its mount.
* Turretinserts other than IX-PH1U or 1X-PHCU cannotbe used. Only
the turret inserts listed above can be replaced. Never attempt to
pull out other turret inserts.
6. Rotate the turret to the Ph1/PhC posttion. Turn the turret insert centering
wrenches clockwise to tighten lightly.
* Do not tighten the centering wrenches excessively as this may
deform the insert mount.
7. Attach the cover and tighten the clamping screws.
a Mounting the Condenser to the Microscope Frame (Figs. 29-35)
| Universal Condenser (IX-LWUCD) (Fig. 29) |
1. Loosen the condenser clamping knob (D.
2. Insert the condenser into the condenser holder fork dovetail. Push the
condenser horizontally onto the condenser holder until the condenser
positioning pin is inserted into the positioning groove of the dovetail.
3. Tighten the condenser clamping knob .
| Condenser (IX-ULWCD) (Figs. 29, 30) |
&laquo;When Using Illumination Column (IX-ILL100LH) Y
1. Loosen the condenser clamping knob (7. (Fig. 29)
2. Insert the turret condenser circular dovetail into the condenser holder
and tighten the clamping knob.
{ When Using illumination Column (IX-ILL30) &gt;
1. Using the Allen screwdriver, loosen the two condenser clamping
screws (1). (Fig. 30)
2. Insert the positioning section on the upper portion of the condenser into
the hole in the condenser mount adapter as far as it will go.
3. Position the turret index window at the front and tighten the condenser
clamping screws (1).
a AA UL A E =
IX50/1X70
&Agrave; | Condenser (IX-SLWCD) (Figs. 31-33) |
cena &copy; This condenser can only be used with the illumination column {IX-ILL30). &gt;
It consists of a slider and a condenser lens. o
== | &lt;
= ( Mounting the Condenser Y o
( 3 1. If the condenser (IX-ULWCD) mounting adapter is mounted to the arm &lt;
== of the illumination column, rotate the adapter counterclockwise and
и remove it.
&laquo; 2. Insert the threaded portion of the condenser () into the illumination
Fig. 31 column and turn the lens in the direction of the arrow until it comes up
against the bracket and is firmly seated. (Fig. 31)
&gt; CInstalling the Ring SIlit Slider )
&copy; Insert the slider into the illumination column (IX-ILL30).
1. Remove the dummy slider from the slider slot on the illumination
column. (Fig. 32)
2. Dropthe frosted filter 4) provided with the illumination column into the
center aperture (3) of the ring slit slider 2). (Figs. 32, 33)
3. With the imprinted side of the ring slit slider 2) upwards, and the
V-groove for the click function towards the rear, insert the slider into the
slot. (Fig. 32)
Fig. 32
Fig. 33
- 11
| | Transmitted Light Universal Condenser (U-UCDB) (Figs. 34, 35) |
(Attaching the Condenser to the UCD Adapter )
1. Using the Allen screwdriver, loosen the two set screws (1) on the UCD
adapter (IX-ADUCD). (Fig. 34)
ASSEMBLY NO)
2. Insert the transmitted light universal condenser (U-UCDB) horizontally into
the adapter until the condenser positioning pin is inserted into the
positioning groove of the mounting dovetail.
NN 3. Tighten the two set screws (1) with the Allen screwdriver. (Fig. 34)
{ Attaching to the Illumination Column )
1. Raise the condenser height adjustment knob (2) to its upper limit position.
(Fig. 35)
2. Loosen the condenser clamping knob (3). (Fig. 35)
3. Insert the mount of the UCD adapter into the condenser holder fork
dovetail (the condenser turret should be positioned on the right hand side).
Push the condenser horizontally onto the condenser holder until the
condenser positioning pin is inserted into the positioning groove of the
mounting dovetail.
N
4. Tighten the condenser clamping knob. (Fig. 35)
{ Attaching Turret Insert Index Stickers )
O If the index stickers are attached as outlined in the instruction manual for
the U-UCDB, they will be difficult to see in case of IX. Affix the stickers as
follows.
e Affix the index sticker provided with the turret insert engaged into the
optical axis at the index position (4) for IX use on the rear side of the
U-UCDB. (Fig. 35)
| Mounting the Polarizer (IX-LWPO) (Fig. 36)
| Universal Condenser (IX-LWUCD)
&copy; The polarizer is used in conjunction with the universal condenser for
performing differential interference contrast observation.
1. Using the Allen screwdriver, loosen the polarizer clamping screw (1).
2. Aligning the pin hole on the polarizer with the positioning pin (2) on the
&laquo; condenser, place the polarizer on the condenser.
Fig. 36 3. Tighten the polarizer clamping screw.
12 &gt;
IX50/1X70
a Mounting the Observation Tube &copy; (Figs. 37-40) -
| Binocular Tube (U-BI9OCT, U-BI90) (Figs. 37, 38) |
1. Using the Allen screwdriver, loosen the observation tube clamping
screw (I) on the observation tube mount. (Fig. 37)
AlgNISSY he)
2. Insert the circular dovetail mount of the observation tube into the
observation tube mount, placing the observation tube so that the inter-
pupillary distance scale numbers 2) are seen right side up. Clamp the
observation tube by tightening the clamping screw (1).
Fig. 37
&copy; Normally, the distance from the surface of the desk to the eyepoint is
approximately 430 mm. If It is desirable to lower the eyepoint position,
the observation tube may be detached, turned 180&deg; and then mounted
on the observation tube mount again. Eyepoint height will be lowered
approximately 30 mm by this procedure. (Fig. 38) |
Fig. 38
В | Trinocular Tube (U-TR30H) (Figs. 39, 40) |
&copy; First mount the intermediate tube (IX-ATU).
1. Using the Allen screwdriver, loosen the observation tube clamping
screw (1) on the observation tube mount. (Fig. 39)
2. Insert the circular dovetail mount of the intermediate tube into the
observation tube mount, placing the intermediate tube so that its upper
surface is flush with the microscope frame. Clamp the intermediate
tube by tightening the clamping screw DD).
Fig. 39
) 3. Using the Allen screwdriver, loosen the observation tube clamping
screw (2) on the intermediate tube’s observation tube mount. (Fig. 40)
4. Insert the circular dovetail mount of the observation tube into the
opening on the intermediate tube, placing the observation tube to point
the binocular eyepieces towards the front. Clamp the observation tube
by tightening the observation tube clamping screw (2).
&deg; 13
ASSEMBLY UNO)
14
x
Fig. 43
9 Mounting the Eyepieces (Fig. 41)
. Remove the eyepiece dust caps.
. Insert the helicoid equipped eyepiece (WH10X-H) into the eyepiece sleeve
with no helicoid {shown on the left in the figure).
. Insert the eyepiece with no helicoid (WH10X) into the eyepiece sleeve
with a helicoid (shown on the right in the figure).
a Mounting 35 mm Single-lens Reflex Camera (Figs. 42-44) |
| Microscope Frame (1X70) N
1.
Using the Allen screwdriver, loosen the two OM camera adapter clamping
screws (1) on the SLR port. (Fig. 42)
Use a sharp object such as the tip of a superfine screwdriver or a
mechanical pencil to remove the plastic cap &copy; from the SLR port.
Aligning the red dot (4) on the SC35 camera and the red dot (3) on the OM
mount of the OM camera adapter, insert the OM mount into the camera
and turn it clockwise until a click is heard. (Fig. 43)
Aligning the red dot 3) on the OM camera adapter's V-groove side with the
red dot (&copy; of the microscope frame's SLR port, insert the OM camera
adapter into the port. (Fig. 44)
Using the Allen screwdriver, tighten the OM camera adapter clamping
screws Q). (Fig. 44)
&copy; When it is required to mount a single-lens reflex camera other than one
with an OM mount, please take your camera mount to a camera shop and
purchase an adapter (T-ring) that will convert your camera mount to a
T-mount. The camera can now be mounted on the microscope frame.
&copy; In addition to single-lens reflex cameras, video cameras having a C-mount
or F-mount can also be mounted. (See page 49.)
O Todetach the mounttrom the single-lens reflex camera, rotate the camera
counterclockwise while pressing down the button (7) on the mount.
(Fig. 43)
Fig. 46
Connecting the Power Cord (Fig. 45)
A Cords are vulnerable when bent or twisted. Never subject them to
excessive force.
A Make sure that the main switch (Dis on “O” (OFF) before connect-
ing cords and cables. (Fig. 45)
&Aacute; Always use the power cord provided by Olympus. If no power cord
is provided, please select the proper power cord by referring to the
section “PROPER SELECTION OF THE POWER SUPPLY CORD&quot; atthe
end of this instruction manual.
1. Before shipment from factory, the voltage selector switch (2) is set to
the 200-240V position in case of the IX-50. In case your local line voltage
is 100-120V, move the switch to the 100-120V position usinga flat-blade
screwdriver.
2. Connect the power cord plug (3) to the AC receptable (9). (Fig. 45)
3. Plug the power cord plug into a wall outlet.
A Connect the power cord correctly and ensure that the ground
terminal of the power supply and that of the wall outlet are
properly connected. If the equipment is not grounded, Olympus
can no longer warrant the electrical safety and performance of the
equipment.
IA Fuse Replacement | | (Figs. 46, 47)
&copy; Before replacing fuses, set the main switch (1) to “O” (OFF) and unplug
the power cord. (The power cord should be unplugged from the AC
receptacle to allow removal of the fuse cassette.)
1. Remove the fuse cassette (2) by inserting the tip of a flat-bladed
screwdriver to squeeze in the claw the left or right side of the fuse
cassette and pulling outward. (Fig. 47)
2. Replace both fuses (3) with new ones. (Fig. 47)
A Use only specified fuses. Use ofimproper fuse could resultin afire.
Applicable fuse (2 fuses)
IX70 + T5A(H) 250V (LITTLEFUSE 215005)
IX50 += T3.15A 250V (LITTLE FUSE 2153.15)
13 Mounting Accessories Such As
Micromanipulator
(Fig. 48)
O Eight tapped holes (1) are provided on the microscope frame for
mounting accessories, such as a micromanipulator, etc. These holes
are covered with plastic dust caps. To remove the caps, insert the tip of
a flat-blade screwdriver sideways into the space between the cap and
the frame.
XxX The applicable screw is M6. The screws can be inserted into the
microscope frame up to a depth of 10 mm. Select the screw length
in accordance with this.
ATANISSV В
15
CONTROLS e
&copy; To explain the various controls, three representative combinations are explained on the following pages.
Interpupillary
distance
adjustment scale
16
CT turret
Focusing ring
SLR port
(IX70 only)
Single-lens
reflex camera
(IX70 only)
Filter holder
Field iris
diaphragm lever
Condenser
centering knob
X-LWUCD
Stage insert plate
IX70-S1F2
1X70-S8F2
Light path
selector dial
A
OT в Condenser
Lamp housing
BB pi Lamp housing
= clamping screw
|
IX-ILLTOOLH
holder clamping
screw
| Condenser height
5 adjustment knob
Condenser
clamping knob
Column tilt
clamping screw
E
\ Magnification
selector knob
j (IX70 only)
|1
[Ту Y-axis travel knob
X-axis travel knob
Fine adjustment
knob
(with rubber cap)
Coarse adjustment
knob
Tension
adjustment ring
W
OL
D
3
e
IX-MVR
with IX-SP
E
CONTROLS В
=
Filter pocket =
Da Y
IX-ULWCD A
Y-axis travel knob
X-axis travel knob
Main switch
ЗУ У
Fuse
Light path selector
18
(X50-S1F
|X50-S8F
WII
(
Slider slot
Lamp port for
reflected
light illuminator
Voltage selector
switch
(IX50 only)
IX50/1X70
Lo FE HW
кз % FE
wa &copy; Bo
&gt; &Euml;
“+1 Brighifield/Phas
(Microscope Frame |X70, [Illumination Column 100W Configuration)
a AO
se a
a HORS
BSE
=&gt;
oo
+
8
(Preparation )
Confirmthatthe frostedfilterisinserted. Filter holder p. 32
Lower the revolving nosepiece. В U “ Coarse adjustment knob p. 24
Y
| Turn ON the main switch. | нннннененненнчннненннчненененнннннененененннненненненненненене Main switch p. 22
Confirm that the light-off switch isnot cernes Light-off switch
activated.
orate the light path selector dial to] nrrrccrrrrrrre Light path selector dial p. 23
When using trinocular observation tube,
move the light path selector knob to = … Trinocular observation tube p. 29
| 100% for binocular eyepieces.
Y
| Place the specimen. | p. 25
| Engage the 10X objective.
Rotate the condenser turretto the “BF”
position. Stop down the aperture IMS... ce. Turret condenser
SIHNC3304d NOILYAY3S9O 10 AYYWINNS В
iris di ‚ 37
diaphragm for low contrast specimens. Aperture iris diaphragm р
у
| Interpupillary distance adjustment | vos . Binocular eyepieces p. 28
T—— Diopter adjustment ring p. 28
| Diopter adjustment | Eyepiece helicoid p. 28
| Bring the specimen into focus. | Coarse and fine adjustment knob р. 24
Stop down and bring the field iris dia-
y phragm image into focus by adjusting
the condenser height. Center the con-| rues Condenser height adjustment knob p. 34
denser and open the field iris diaphragm Condenser centering knobs p. 34
image so that it circumscribes the field
of view.
Y
| Engage the objective to be used. |
For phase contrast observation, rotate
the condenser turret to engage the ring peices
| Jage | Turret condenser
slit that matches the objective in use.
For phase contrast observation, center, ~~ Ring slit slider 36
the respective ring slit. (VS centering screws and CT p.
Y .
[ Bring the specimen into precise focus. |&quot; teren Coarse and fine adustment knob 0.24&quot;
Adjust the correction collar of 40X, or
60X objectives according to the TICKET Objective correction collar p. 39
ness of the vessel bottom.
Y
Adjust the light intensity. | … Light intensity lever p. 22
For brightfield observation, adjust the
aperture iris diaphragm. For phasecon-| er Aperture iris diaphragm lever on
trast observation, open the aperture iris turret condenser | p. 37
diaphragm.
-—— Engage the filter(s) to be used. EE Filter holder p.32
y
( Observation )
SUMMARY OF OBSERVATION PROCEDURES В
42 Brightfield/Phase Contrast Observation
( Preparation )
(Microscope Frame 1X50, Illumination Column 30W Configuration)
Confirm that the frosted filter is inserted.
Lower the revolving nosepiece.
Filter holder
Coarse adjustment knob
q
| Turn ON the main switch. |
srraceeennenten cena nus Main switch
— Move the light path selector leverto &quot; | &quot;
матотанинвавинантнншцваавичнпеяненазанатт Light path selector lever
When using trinocular observation tube,
move the light path selector knob to 100%
for binocular eyepieces.
e Trinocular observation tube
у
| Place the specimen. |
| Enga ge the 10X objective. ||
Set the condenser turret to the brightfield
(BF) position. Stop down the aperture iris dia-
phragm if the contrast of specimen is low.
ere Condenser iris diaphragm lever
r
| Diopter adjustment
| Interpupillary distance adjustment | meando nremmaaArrenD ranita eN
PRECCTTECECELLLEL EE ELLE EEES Binocular eyepieces
eet teeter rere rere nenees Diopter adjustment ring
Eyepiece helicoid
| Bring the specimen Into focus. N ene ‘|
| Engage the objective to be used. |
For phase contrastobservation, en-
the objective in use.
gage thering slitslider that matches |i,
Coarse and fine adjustment knobs
ernonaninceaoe or eneneneanceneenacanereD Turret condenser
For phase contrast observation, center the
respective ring slit.
Ring slit slider centering screws
and CT
y
Bring the specimen into precise
focus.
.... Coarse and fine adjustment knobs
Adjust the correction collar of 40X objec-
tives, etc. according tothe thickness of the
vessel bottom.
preenenericanaeereeereaoeeneneneenes Objective correction collar
y
| Adjust the light intensity. |
secaneoreamar escena Nano Light Intensity lever
For brightfield observation, adjust the ap-
erture iris diaphragm. For phase contrast
observation, open the aperture iris dia-
phragm.
Aperture iris diaphragm lever on
pp turret condenser
.. Filter holder
| | Engage the filter(s) to be used. |.
\ 4
( Observation )
. 32
.22
.23
.29
.25
. 37
.28
.29
.29
.24
. 36
. 36
. 24
. 39
‚22
. 37
‚ 32
20
IX50/1X70
4-3 Transmitted Light Nomarski DIC Observation
7
(When Using the Universal Condenser IX-LWUCD)
(Preparation)
Remove analyzer, polarizer, transmitted
light differential interference contrast
prism (U-DICT) from the light path.
Turret condenser
Analyzer, U-DICT
Confirmthatthe frostedfilterisinserted.
Lower the revolving nosepiece.
Filter holder
+ Coarse adjustment knob
| Place the specimen. |
| Engage the 10X objective. |]
Y
Turn ON the main switch. Main switch
switch.
Confirm that the light-off switch is not |... creer Light-off switch
activated.
Rotate the light path selector dial to Light path selector dial (1X70)
“а” &copy; 191 pa | Light path selector lever (1X50)
When using trinocular observation tube, Tri lar ob b
move the light path selector knokb to jee rinocular observation tube
100% for binocular eyepieces.
y
Rotate the condenser turret to the “BF”
posttion. Stop down the aperture iris
Turret condenser
ARENA IRENE ATEO Aperture Iris diaphragm
| diaphragm for low contrast specimens.
у
[ Interpupillary distance adjustment |
- Binocular observation tube
Diopter adjustment ring
| Diopter adjustment |
[ Bring the specimen into focus. |
Eyepiece helicoid
eevacarenceneraren tenen enenerenneneene. Coarse and fine adjustment knob
Stop down and bring the field iris dia-
phragmimage into focus by adjusting the
condenser height. Center the condenser
and open the field ins diaphragm image
so that it circumscribes the field of view.
Condenser height adjustment knob
Condenser centering knobs
— Engage the analyzer and polarizer.
y
Adjust until complete extinction is ob-
tained (“crossed Nicol” position).
| Engage the objective to be used. |
У
Rotate the condenser turret to engage the
DIC optical element that matches the ob- |--.---
| eneicaoiaoecere rara aerceneeeernee&oacute;a Polarizer rotation knob
reas Turret condenser
jective in use.
Y
| Bring the specimen into precise focus. | ancssrenrrensass neue
ннемненнееееенененеенеенение Coarse and fine adjustment knob
Adjust the correction collar of 40X or 60X
etc. objectives according to the thick-
PS Objective correction collar
| ness of the vessel bottom.
Y
| Adjust the light intensity. |
RR. Light intensity lever
——— Adjust the aperture iris diaphragm.
Y
O To adjust the background color contrast, rotate the transmitted light differential interference contrast prism control knob.
Aperture iris diaphragm on turret
&quot;condenser
Do
о оо &copy;
.41
32
. 24
‚ 22
‚ 23
‚ 29
25
41
37
28
28
28
24
. 34
‚ 34
42
‚ 41
‚ 24
39
22
37
SIHNC3204d NOILVAYS9O JO AHVININNS В
21
Fig. 50
USING THE CONTROLS on
&gt;)
J
Q
с
—
Turning ON the Light Souce (Fig. 49)
Press the main switch (1) at the rear of the microscope frame to ON.
(ON is when the switch is set to “ 1”; OFF is when the switch is set to
&quot;Q”)
Voltage Adjustment | (Figs. 50, 51)
Sliding the light intensity lever (7) upward increases the voltage, increas-
ing the light intensity.
&copy; Move the light intensity lever to find the optimal brightness. Noting the
position of the light intensity lever relative to the dots e to the right of the
light intensity lever facilitates duplication of the previous light intensity
setting.
&copy; For accurate color temperature adjustment, use the color temperature
meter of the camera unit.
| Microscope Frame (1X70) |
&copy; For color photomicrography, the voltage should be approximately at the
level indicated by the camera symbol ( 81 ). The voltage will be ap-
proximately 9V.
( Light-off Switch y
&copy; When observing very weak fluorescent specimens using a high-sensi-
tive video camera, suchas a C-CCD or SIT camera, the specimen position
is determined using transmitted light. Then, the light-off switch is used
to temporarily turn off the transmitted light during video camera obser-
vation of the specimen.
1. When the light-off switch (D is depressed, the LED indicator will light up
and the transmitted light illumination will be turned off.
2. When the switch is pressed again, the switch lock is released, the LED
indicator goes out, and the transmitted light illumination will return to its
original brightness.
* Please note that ifthe light-off switch is left in the locked position,
the illumination will not turn on, even if the main switch is set to
“1” (ОМ).
| Microscope Frame (1X50) |
&copy; Is not provided with a camera symbol and light-off switch.
22
CD
LL
Light Path Selection
(Figs. 52, 53)
| Microscope Frame (1X70)
(Fig. 51)
Fig. 52
Dial position
&lt;
&lt;
Binocular eyepieces
SLR port
20%
80%
100%
symbol a
Light path [&copy;] [sP]([sP])
Side port — — 80% (100%)
20% ( —)
Select the light path by rotating the light path selector dial (1) to the
position indicating the desired observation method.
Brackets () indicate microscope frame IX70-S1F2.
&copy; A vibration damping mechanism is employed to ensure that vibration is
reduced to an absolute minimum during light path change provided the
light path selection dial is rotated slowly.
&copy; The side port can be used for mounting photometric equipment, a camera
unit via a straight photo tube, or a video-camera via a video-adapter.
Fig. 53
| Microscope Frame (IX50)
(Fig. 53)
Turn the light path selector lever (1) to the position indicating the desired
observation method.
symbol
Lever position
a
&lt;
Binocular eyepieces
100%
Light path
Side port — 80% (100%)
20% (—)
Brackets () indicate microscope frame IX50-S1F.
unit via a straight photo tube, or a video camera via a video adapter.
Magnification Change
(Fig. 54)
&copy; The side port can be used for mounting photometric equipment, a camera
Fig. b4
| Microscope Frame (1X70)
|
When the magnification selector knob (1) is pulled out, the magnification
will be 1.5X. When the knob is pushed in, the magnification will be 1X.
* When using the IX-CA2X, and depending on the ambient tem-
perature, focus may be lost when the magnification is changed. In
this case, readjust the focus. When observing with the IX-CA2X,
slight vignetting may occur at the periphery of the field of view.
Vignetting will disappear if used in combination with the IX-EPA.
| Microscope Frame (1X50)
Not equipped with magnification changer.
STOY1NO9 3HL ONISN В
23
USING THE CONTROLS В
24
( 5-2 Focusing Adjustment Knobs
Fig. 55
Fig. 56
mana a — — - X .- = но = нок a TIA DANA DADA AAA ee a те ene
Adjusting the Coarse Adjustment Knob Tension (Fig. 55)
O Always adjust the coarse adjustment knob tension using the tension
adjustment ring 0).
The coarse adjustment knob tension is preadjusted for easy use. However,
if desired, one can change the tension using the tension adjustment
ring. Turning the ring in the direction of the arrow increases tension, and
vice versa.
The tension is too low if the revolving nosepiece drops by itself or focus
is quickly lost after adjustment with the fine adjustment knob. In this
case, turn the ring in the direction of the arrow to increase tension.
Using the Fine Adjustment Knob Rubber Cap (Fig. 56)
&copy; Ordinarily, the fine adjustment knob is used with the rubber cap attached.
However, if you feel the space between the knob and the stage controls
IS insufficient, the cap may be removed by pulling it straight out.
The cap makes it easier to turn the fine adjustment knob in small
increments to obtain more precise focusing.
Pre-focusing Lever | (Fig. 57)
&copy; The pre-focusing lever ensures that the objective does not come in
contact with the specimen and simplifies focusing.
After focusing on the specimen with the coarse adjustment knob, turn
this lever (1) in the direction of the arrow to set an upper limit on the
objective movement.
After changing specimens, refocusing is easily accomplished by turning
the coarse adjustment knob to reach the pre-focused position, then
making fine adjustments with the fine adjustment knob.
O Objective movement with the fine adjustment knob is not affected by
this pre-focusing lever.
IS
ls
ali
IX50/1X70
N
|
}
Specimen Placement (Figs. 52-62)
| Stage (IX-SFR, IX-SVL) (Fig. 58) |
Place the specimen in the center of the stage.
&copy; Inthe case of a slide glass specimen, place the specimen with the cover
glass facing down.
&copy; If the specimen is prone to slide on the stage, attach the stage clips
(U-SCL) ( and clamp the specimen down with the clips.
| Mechanical Stage (IX-MVR) (Figs. 59-62) |
&copy; 96-well and 24-well microtiter plates, etc. are held in place by the speci-
men holder. Microtiter plates with dimensions of Max. 136 mm x 92 mm
can be accommodated in this way.
1. Open the spring-loaded finger of the specimen holder (4) and slide the
microtiter plate into the holder frame. Gently release the curved finger to
clamp. (Fig. 59)
&copy; To secure other vessels than microtiter plates, various optional holders
are available. A Terasaki plate holder 2) is available for holding Terasaki
plates (72-well, 60-well). When using this, it is necessary to replace the
stage scales with those provided with the plate holder. Petri dish holder
(3) is available for 35 mm, 54 mm, and 60 mm diameter Petri dishes, and
a slide glass holder @) is available for holding slide glass. (Fig. 60)
STOYLNOI 3HL DNISN a
25
USING THE CONTROLS В
26
LE EE &copy; ово
Fig. 61
{ Adjusting the Scales Y
&copy; Before using the mechanical stage, adjust the provided scales. The
scales are designed to match the X,Y coordinates of a 96-well microtiter
plate, thus making it easy to read the coordinates of a well under
observation.
1. Operate the Y-axis and X-axis travel knobs to bring a well at the center
area of the 96-well microtiter plate (example: D, 6) into the center of the
light path.
2. Loosen the X-axis scale clamping knobs (5) and slide the scale to align the
X-index e (6) with the scale index e (7). Then tighten the X-axis scale
clamping knobs. (Fig. 61)
3. Loosen the Y-axis index clamping knob (8) and move the scale to align the
scale index e () with the index e (9. Then tighten the Y-axis index
clamping knob. (Fig. 62)
Moving the Specimen (Fig. 63)
| Stage (IX-SFR, IX-SVL)
1. To move the specimen to adesired position, rotate the X-axis knob (1) and
the Y-axis knob D.
&Oacute; When the index mark of the upper stage is aligned with the index line 3)
provided on the substage, the center of the stage insert plate aperture
Is almost in the center of the optical axis. Use this as a guideline when
moving the specimen. Travel area is 50 mm x 50 mm.
&copy; !f the revolving nosepiece is rotated to change the objective immediately
after observing the specimen with an objective having a short working
distance (0.5mm or less), the objective may impinge on the stage insert
plate. If objectives with a short W.D., or oil immersion objectives, are
frequently employed, using the optional stage insert plate with 50 mm
diameter aperture (IX-CP50) will greatly reduce the risk of the objective(s)
hitting the stage insert plate.
* The stage (IX-SVL) travel area is 43 mm (Y) x 50 mm (X).
| Attachable Mechanical Stage (IX-MVR) |
Specimens are moved in the same manner as outlined in Step 1 above.
&copy; The stage travel area is 85 mm (Y) x 130 mm (X).
| = Ww
Db =
&laquo;M CD
MD
0 &lt;
= o
rr
—
ho
IX50/1X70
Connecting the Grounding Wire (Fig. 64)
| Stage (IX-SFR, IX-SVL) |
&copy; A grounding wire can be attached to the stage for electrophysiological
experiments, etc.
Prepare a grounding wire (1) and one M4 screw (2), and attach the wire
as shown in Fig. 64.
* The screw hole may be covered by a paint covering, etc. There-
fore, before fastening the grounding wire, screw in and unscrew
the M4 screw several times until the screw hole’s metal portion
is exposed. Then tighten the screw to put the grounding wire in
firm contact with the stage.
Adjusting the Tension of X-axis and Y-axis knobs (Fig. 65)
| Stage (IX-SVL) |
&copy; The tension of the X-axis and Y-axis knobs can be individually adjusted.
1. Using the provided Allen wrench, loosen the two set screws (1). While
holding the stage stationary, turning the knob in the direction of the
arrow increases tension, and turning it in the opposite direction reduces
tension.
2. After adjusting the tension, tighten the two set screws again.
* If the tension is adjusted too tight, or too loose, the image may
jump or show backlash during stage travel.
27
}
STOYLNOI ЭНА DNISA 2
roy sy
M eg O on amar hy EER RE ET TE EET TEE TER EA
EEE A E EEE Te ! ROA 86 ERA NO Se Е В Fogg : a e DE Ae
+ A Fa ET A LE Ra RE EE TE FA e MEA NE „8 LEE SEE CETTE aT . ERT I FEE get Fe EE an
; ; A a A ; EEES TEE EE E. EEE Era EP 8 у EA E
N ENS . , ‘ts CEN Г Boyt OF Ty BEE ETT EE SEE TE EST AE eT, 5 8 5 N ES TE # в 788
- E. FE: ME &lt; Ga EE en Fam NN Ia A pe gt Feet RE eh LN
wy get Egg Ea Reed ee wm Bog af EEE pe By a Fg eT RE a TE BE Se at FFF RG
er aE PAR BRE Me ESTE A A A E E o Tas Ea ie fF RE
Bh ten dh o EA E a AENA DEA, „8 Sell FREE ELE wT dae aida ind ETRE LAY, Hi rem
) Interpupillary Distance Adjustment (Fig. 66)
While looking through the eyepieces, adjust the binocular vision until the
left and right fields of view coincide completely. The index dot e indicates
the interpupillary distance.
&copy; Note your interpupillary distance so that it can be quickly duplicated.
Fig. 66
e Diopter Adjustment a В (Figs. 67-71)
Microscope Frame (1X-70) (Figs. 67-69) |
1. Pull out the photo frame (1) to engage the photo mask into the light path.
(Fig. 67)
2. Looking through the right-hand light path's helico:d eyepiece, turn the
diopter adjustment ring 2) on the eyepiece sleeve until the reticles in the
center of the photo mask are seen as two distinct sets of reticles in the
field of view. (Figs. 68, 69)
3. Looking through the left-hand light path's eyepiece, turn the diopter
adjustment ring 3) on the eyepiece until the reticles in the center of the
USING THE CONTROLS [@)
photo mask are seen as two distinct sets of reticles in the field of view.
* When turning the eyepiece's diopter adjustment ring, use your
other hand to hold the lower part of the eyepiece stationary.
4. Rotate the coarse and fine adjustment knobs (4) to bring the specimen
into focus. (Fig. 67)
J LL
Le ‘и чи
LL
| Microscope Frame (IX-50) (Figs. 70, 71) |
1. Looking through the right eyepiece, turn the diopter adjustment ring (1)
on the eyepiece sleeve until the periphery of the field of view is sharp.
(Fig. 70)
&copy; Diopter adjustment can also be performed when eyepiece micrometers
are used.
2. Looking through the eyepiece, rotate the coarse and fine adjustment
knobs (2) to bring the specimen into focus. (Fig. 71)
3. Looking through the helicoid eyepiece, turn the diopter adjustment ring
&reg; on the eyepiece to focus on the specimen. (Fig. 70)
* When turning the eyepiece’s diopter adjustment ring, use your
other hand to hold the lower part of the eyepiece stationary.
Observation Tube Light Path Selection (Fig. 72) -
| Trinocular Observation Tube (U-TR30H) |
Slide the light path selector knob (M) to select the desired light path.
&copy; The selector knob is ordinarily at the middle position. With dark speci-
mens, push the knob in. If additional light is needed for television or
photomicrography, pull the knob out.
Light path as
selector knob Symbol Intensity ratio Application
100% for Observation of
Pushed in [=] q binocular eyepieces | dark specimens
20% for Observation of
oo binocular eyepieces, | bright specimens,
Middle position [= Jaro 20% for YEP aay
TV/photography TV observation
100% for Photography,
Pulled out [| [0] TV/photography TV observation
IX50/IX70
Cc
&copy;
&laquo;&lt;
&copy;
—
I
m
O
O
&lt;&gt;
—
x
O
г
о
29
USING THE CONTROLS В
30
Fig. 73
Fig. 74
a How to Use the CT (Bertrand Lens) Turret (Fig. 73)
[ Binocular Tube (U-BI90CT) |
1. To use the CT turret (7, turn the knurled ring with a finger to select the
setting corresponding to the indication (O-CT-O-S). When selecting the
setting, make sure the turret is correctly positioned at a click-stop.
(Set the magnification selector knob on the IX70 microscope frame to 1X.)
Turret symbol Application
O (2 positions) | Nothing is engaged into the light path (Normal observation)
The CT lens is engaged into the light path and the objective exit
pupil can be observed.
CT Used when centering the ring slit and phase annulus in phase
contrast and adjusting the aperture iris diaphragm.
The light excluding plate is engaged into the light path to block
с extraneous light from entering via the eyepieces. Used during
photomicrography to prevent extraneous light from affecting
the exposure.
2. To bring the exit pupil image of the objective into focus when using the
CT lens, turn the focusing ring @ to adjust.
| Binocular Tube (U-BI90), Trinocular Tube (U-TR30H) |
These observation tubes are not equipped with a built-in CT lens. Use a
focusing telescope (U-CT30).
e Engage the 10X objective and obtain approximate focus. Replace the
objective with the focusing telescope.
oe Rotate the knurled portion of the focusing telescope and bring the
objective exit pupil into focus.
Using the Eye Shades Fig. 74)
| When Wearing Eyeglasses |
Use with the eye shades in their normal folded down position. This will
prevent the eyeglasses from being scratched.
| When Not Wearing Eyeglasses |
Extend the folded eye shades in the direction of the arrow for efficient
use of the eye shades.
3)
the
the
1X.)
Fig. 75
IX50/1X70
Tilting the lumination Column (Fig.7)
| Illumination Column (I1X-ILL100LH) |
&copy; When replacing large specimens, placing a micromanipulator, or replac-
ing a patch clamp electrode, working space can be created by tilting the
illumination column,
&copy; Even with the illumination column tilted, the specimen surface will be
illuminated, which is convenient for rough confirmation of the specimen
location or initial positioning when placing the specimen.
. Using the Allen screwdriver, loosen the column tilt clamping screw (1) by
rotating it approximately 11 rounds in the direction of the arrow.
. Holding the illuminator attachment’'s upper front side, slowly tilt the
illumination column backward. Vibrations should be avoided. Accordingly,
always support the illumination column with a hand and tilt slowly and
gently. Toreturn the column to ts original position, reverse this procedure.
x |f the reflected light illuminator attachment is mounted, and the
condenser holder is left rotated to the back of the column, the
condenser holder will contact the reflected light illuminator attach-
ment lamp housing if the illumination column is tilted. Accord-
ingly, do not attempt to tilt the illumination column while the
condenser holder remains situated at the back of the column.
A When tilting the illumination column upwards or downwards,
make sure not to catch your fingers in the hinge joint.
sea
* The tilt clamping screw should normally be tightened during use.
the If the microscope is used while the screw is loosened, make sure
that the illumination column does not accidentally tilt during use.
the A When moving or transporting the microscope, always do so with
the tilt clamping screw tightened.
* Ifthe lamp housing unitis remodeled, and a heavy module, such
as a high-intensity lamp housing, Is installed, always use the
microscope with the tilt clamping screw tightened.
4)
will
lent
—e
STOYLNO3 3HL DNISN
31
USING THE CONTROLS В
Mounting Filters (Figs. 76-79)
| lllumination Column (IX-ILL100LH) (Figs. 76-78) |
&copy; 45 mm diameter, maximum 6 mm thick filters can be used. Various
filters, such as the provided frosted filter, the color temperature conver-
sion filter (LBD), the green interference filter {IF550), and ND filter, can
be mounted.
1. Place a finger on the milled section (1) of the filter holder and lift the filter
holder. (Fig. 76)
&gt;
2. While pressing down the protrusion of the plastic filter holder (2), insert
the filter. (Fig. 77)
x Holdthe filter by its edge to avoid leaving fingerprints orsmudges
on the filter surface.
A After the illumination has been turned ON, the filter will be very
hot. Only replace filters after setting the main switch to “O” (OFF)
and allowing the filter holders and filters to cool.
3. Engage the filters into the light path by moving them in the direction
indicated by the arrow. (Fig. 78)
* Unless maximum intensity is required, always leave the frosted
filter engaged in the light path.
| Illumination Column (IX-ILL30) (Fig. 79) |
1. Fordetallsonhowtomountfilters, see “ lllumination Column {IX-ILL100LH)&quot;
above.
2. Engage the filters into the light path by moving them in the direction
indicated by the arrow.
* Unless maximum intensity is required, always leave the frosted
filter engaged in the light path.
—
-n
JUS
/ег-
san
Iter
sert
jes
ery
FF)
Jon
ted
Ну”
tion
ted
IX50/1X70
=
Field iris daphragm
Fig. 81
Using the Field iris Diaphragm (Figs. 80, 81)
[ INumination Column (IX-ILL100LH) |
&copy; The field iris diaphragm lever (1) is used to adjust the diameter of the
illuminating beam in accordance with the objective in use. Adjust the
diaphragm so the field of view is circumscribed by the field iris diaphragm
to exclude stray light.
&copy; То limit specimen damage or fading when observing living cells or
fluorescent specimens, the field iris diaphragm may be stopped down.
The field iris diaphragm can be opened/closed by moving the field iris
diaphragm lever () rightward/leftward.
| Illumination Column (IX-ILL30) |
Is not provided with a field iris diaphragm. (It has a fixed diaphragm.)
S1OHILNO9 3HLI ONISA В
33
USING THE CONTROLS a
34
| 5-6 Condenser
Centering the Condenser (Figs. 82, 83)
| Universal Condenser (IX-LWUCD), Condenser (IX-ULWCD) (Fig. 82)
{Mumination Column {IX-ILL100LH) )
1.
a &gt; OU N
Rotate the turret (1) to select the “BF” brightfield observation setting (no
optical element engaged).
. Turn the aperture iris diaphragm lever 2) to open the diaphragm.
. Move the field iris diaphragm lever @) to the fully open position (@—&gt;O).
. Engage the 10X objective and bring the specimen into focus.
. Using the field iris diaphragm lever, stop down the field iris diaphragm until
its image is just inside the field of view.
. Turn the condenser height adjustment knob @) to bring the field iris
diaphragm image into focus.
. While gradually opening the field iris diaphragm image, rotate the con-
denser holder's condenser centering knobs (5) to adjust so that the field iris
diaphragm image 15 centered in the eyepiece field of view.
. To check centration, open the field iris diaphragm until its image touches
the perimeter of the field of view. If the image is not precisely inscribed in
the field of view, center the condenser again.
&copy; When used for actual observation, open the field iris diaphragm until its
Image just circumscribes the field of view.
*X When the condenser (IX-ULWCD) is used in combination with 40X
or higher power objective, the field iris diaphragm cannot be seen
in the visual field.
* The field iris diaphragm cannot be seen in the field of view when
the condenser (IX-LWUCD) is used in combination with a 100X
objective.
y (no
&gt;).
until
1 iris
con-
diris
ches
ed in
til its
40X
seen
/hen
100X
IX50/1X70
Transmitted Light Universal Condenser (U-UCDB) (Fig. 83) |
* For operation of the U-UCDB, refer to the provided instruction
manual (BX-UCDB).
Since this condenser is used in its inverted condition, the turret inserts
may fall out if the condenser is subjected to shock or impact. In particular,
be careful when tilting the condenser holder.
(Illumination Column (X-ILL100LH) Y
1.
м оо с A
10.
Loosen the condenser height fine adjustment knob @) Бу turning it
counterclockwise. Then push it all the way to the rear.
Rotate the condenser height adjustment knob (2) in the direction of the
arrow to lower the condenser to its lowest position.
Rotate the turret 3) to select the “BF” brightfield observation setting
{no optical element engaged.)
Turn the aperture iris diaphragm lever (4) to open the diaphragm.
. Move the field iris diaphragm lever (5) to the fully open position. (&sect;— OO).
. Engage the 10X objective and bring the specimen into focus.
. Using the field iris diaphragm lever, stop down the field iris diaphragm
until its image is just inside the field of view.
. Slowly move the condenser height fine adjustment knob (1) to the front
to bring the field iris diaphragm image into focus. When the image is in
focus, clamp the knob by turning it clockwise.
. While gradually opening the field iris diaphragm image, rotate the
condenser holder's condenser centering knobs (6) to adjust so that the
field irs diaphragm image is centered in the eyepiece field of view.
To check centration, open the field iris diaphragm until its image touches
the perimeter of the field of view. If the image is not precisely inscribed
in the field of view, center the condenser again.
&copy; When used for actual observation, open the field iris diaphragm until its
image just circumscribes the field of view.
&copy; When replacing the specimen or applying oil, use the condenser height
adjustment knob to first raise the condenser and then lower it to its
lowest position. If this is done, it should be unnecessary to adjust the
condenser centration or focus again.
STOYINOS3 3HL DNISN
35
USING THE CONTROLS
36
Fig. 85
Centering the Ph ntrast Ring Slit
Attachment aseCo ng &gt;t (Figs. 84-87)
. Engage the phase contrast objective and bring the specimen into focus.
. When the U-BI90OCT binocular observation tube is used, rotate the CT
turret (1) to select the “CT” position. When observation tubes other than
the U-BI9OCT are used, remove an eyepiece and replace it with the
focusing telescope (U-CT30). (Fig. 84)
. Engage the ring slit in the condenser that matches the phase contrast
objective.
. Turnthe focusing ring@ (the knurled ring in case of the focusing telescope)
and focus on the objective phase annulus (4) matching the condenser ring
slit 3). (Figs. 84, 85)
. The centering operations for each unit are outlined below.
* Ring slit ghost images may appear. In this case, select the brightest
ring to superimpose over the phase annulus.
* In case of thick specimens, or if the specimen is moved or replaced,
the concentric superimposition of the ring slitand phase plate may
be disturbed. In this case, and if the observed image contrast is
poor, perform steps 1 through 4 again to re-adjust.
. After centering, rotate the CT turret to return the turret to the &quot;0&quot; position.
If the focusing telescope (U-CT30) was used, replace it with the eyepiece.
Universal Condenser {(IX-LWUCD), Condenser (IX-ULWCD} (Fig. 86) |
Using the optical element centering wrenches (1), rotate the phase
condenser ring slit centering screws (marked A) until the ring slit image is
superimposed over the objective phase annulus.
* To obtain best contrast, re-centering may be required ifthe bottom
of a culture vessel is not completely flat. Perform centering in the
order of low to higher power objectives.
[ Condenser (IX-SLWCD) (Fig. 87) |
Fig. 87
&raquo; Rotate the ring slit slider's centering knobs (1) to superimpose the ring slit
image over the phase annulus of an objective compatible with the ring slit.
Ring slit . Compatible objective
PhL UPlanF14XPh
PhC CPlan10XPh, LCAch20XPh, CPlanFI10XPh
IX50/IX70
87) 3 70~80% Using the Aperture Iris Diaphragm (Fig. 88)
a | 30 — 20% | Ш
о : | &copy; In general, the potential resolving power of an objective is fully utilized
us. В If the diaphragm is stopped down to correspond with the numerical
e CT aperture (N.A.) of the objective.
en &copy; Depending on the specimen, image contrast may be improved, or focus
&copy; depth increased, by keeping the aperture iris diaphragm stopped down
Obiecti я a little during observation or photomicrography. In general, a good image
ective pupil . . . . . .
trast &laquo; PP Is obtained if the diaphragm is stopped down to 70-80% of the numerical
| aperture of the objective. Stop further down for less contrasty speci-
cope) &Euml; Fig. 88 mens.
rring e O To check the position of the aperture iris diaphragm, remove the
| eyepieces. Looking through the eyepiece sleeves, the aperture iris
{ diaphragm image and the objectives exit pupil can be seen.
htest | | &copy; When using the binocular observation tube (U-BI90CT)}, and setting the
; turret to “CT”, or when using a focusing telescope (U-CT30), the
iced, &amp; aperture iris diaphragm can be observed in the same way.
may &amp;
ist is
. . . . a na 00e o - CC
~ Jl Adjusting the Tension of the Condenser (Ра. 89 о
ition. Height Adjustment Knobs 22&deg; 19. 89) 5
lece. —
I
| Illumination Column (IX-ILL100LH) | u
36) | &copy; The tension of the condenser height adjustment knobs (1) is preadjusted =
36) for easy use. However, if desired you can change the tension. Holding 3
nase the right condenser height adjustment knob stationary with the right &gt;
ge is hand, rotate the left condenser height adjustment knob until the tension
of the knobs is as desired.
tom
| the
Tilting the Condenser Holder (Fig. 90) |
37)
y slit | Illumination Column (IX-ILL100LH) |
slit &copy; To create working space and facilitate specimen replacement, micro-
manipulator positioning, or mounting an objective through the hole in the
stage, tilt the condenser holder upwards.
1. Placing a hand against the bottom of the condenser holder, press upwards.
2. The tension of the tilt can be adjusted by rotating the adjustment screw
( using a turret insert centering wrench or the shank of the Allen screw-
driver.
* Adjust the tension so that the tilted condenser will not drop down
by itself.
* When returning a tilted condenser to its original position, do so
gently and slowly.
&gt; 37
wn
|
O
EC
|
2
O
O
Ш
T
|
O
=
о
2
38
a Rotating the Condenser Holder (Fig. 91)
| lllumination Column (IX-ILL100LH)
&copy; When replacing large specimens, placing a micromanipulator, or placing
a patch clamp electrode, working space can be created by turning the
condenser holder all the way to the rear of the illumination column.
It is convenient for the observation of large culture vessels. Using the
Allen screwdriver, loosen the clamping screw (1). Rotate the condenser
holder.
* When the condenser holder is rotated out of the way, specimens
with a height of up to 230 mm can be placed on the stage surface.
—
—
a o
91)
acing
g the
g the
anser
nens
face.
ne
Toe
= r
e 1 ect es: ce
; ' } PE =
Чен нео лей ref RE -
rE EEE a
PE. ANNA wr &quot;E.
: Pre a = 5 + 5
Correction Caps
(Glass)
CAP-G
&gt;
| seseo-
]
(Plastic)
CAP-P
Eso: reo if
L
Y
50+
Fo
Nd 09&deg;07 XY
нченот
SAdIALAO
Objective
Fig. 92
Correction Caps for Objectives
The following objectives can be used with correction caps. By attaching
the caps in accordance with the bottom thicknesses of various vessels,
optimum performance is obtainable with glass vessels, and plastic vessels.
{ Objective Types )
IX50/1X70
(Fig. 92)
Brightfield/
Differential interference
Phase contrast/
Brightfield
Correction collar
LCPLFL20X
LCPLFLAOX
LCPLFL6OX
LCPLFL20XPh
LCPLFL40XPh
LCPLFL60XPh
None
Available
Available
Provided as standard
accessory
Correction cap:
CAP-G1.2
Provided as standard
accessory
Correction cap:
CAP-P1.1
* As shown in the above table, the brightfield/differential interference
objectives are delivered with the correction cap “CAP-G1.2” for use with
glass vessels, and the phase contrast/brightfield objectives are delivered
with the “CAP-P1.1” as standard accessory. Confirm the compatibility of
the culture vessel in use.
(Correction Cap Types (usable with all objective types) Y
Name
Applicable vessel thickness range (mm)
CAP-GO.5
CAP-G1.2
CAP-G2
Glass
vessels
0-1.0
0.7-1.7
1.5 - 2.5
CAP-PO.5
CAP-P1.1
CAP-P2
Plastic
vessels:
0-1.0
06-106
1.5-2.5
Correction Collar Adjustment
1. The correction collar is effective with 0 to 2.5 mm thick vessel bottom
thicknesses.lIf the thickness of the vessel bottom is known, match the
correction collar to the thickness of the vessel bottom by using the scale.
2. How to find the optimum position for optimum image resolution and
contrast.
&copy; If the thickness of the vessel bottom is unknown, the optimum position
for the correction collar can be obtained by judging the image resolution.
When a satisfactory image is not obtained after focusing, rotate the
correction collar to the right or the left and refocus to compare the image
at both sides. Return the collar to the the position yielding the improved
image; then starting from this position, further rotate the collar slightly to
the right or left, refocusing each time, to compare the image at each
position. Repeat this procedure until the position with the optimum image
is found.
STOYLNOO IHL DNISN
39
USING THE ..
40
Fig. 93
Using Immersion Objective (Fig. 93)
. Using a low power objective, bring the specimen into focus.
. Rotate the revolving nosepiece to engage the oll immersion objective.
. Remove the specimen and move the stage insert cut-out (0) close to the
objective front lens. Apply a drop of the provided immersion oil to the
front lens of the objective. Place the specimen and rotate the fine
adjustment knob to bring the specimen into focus.
* Use as little oil as possible. Immediately after use, remove the oil.
x If the oil contains air bubbles, the image will be degraded. Make
sure the oil is free of air bubbles.
(1) Tocheck forairbubbles, remove the eyepieces. Completely open the
field iris diaphragm and aperture iris diaphragm. When looking at the
objective exit pupil {looking like a bright circle), any air bubbles can
be seen.
(2) To remove air bubbles, slightly rock the revolving nosepiece to
engage and disengage the oil immersion objective once or twice.
. After use, wipe away the immersion oil at the objective front lens with
gauze slightly moistened with EE-6310 or a mixture of ether (70%) and
alcohol (30%).
* Excessive amounts of alcohol may dissolve the adhesive of the
objective.
&copy; The same procedure is applicable when using a water immersion
objective.
IX50/IX70
DIFFERENTIAL INTERFERENCE CONTRAST OBSERVATION ~~
Differential Interference Contrast Observation \
. 93) a | Universal Condenser (IX-LWUCD) (Figs. 94-99) |
{Installing and Adjusting the Analyzer and Polarizer )
1. Remove the dummy slider from the revolving nosepiece.
ective.
2. Align the analyzer (U-ANT) (1) and place it into the opening in the trans-
&agrave; to ie mitted light differential interference contrast slider (U-DICT) (2. {Fig. 94)
to the
1e fine
heoil.
‚ Маке
sen the 3. Insertthe transmitted light differential interference contrast slider (U-DICT)
Jat the (3) with the analyzer facing downwards and engage it into the light path.
es can Turn the clamping knob @) to clamp. (Fig. 95)
&copy; When using the IX-AN analyzer &copy;, insert it into the slider slot (6) under the
ece to revolving nosepiece. Insert as far as the click-stop. In this case, the
wice. analyzer U-ANT is not used. (Figs. 95, 96)
1S with O When performing simultaneous reflected light fluorescence and trans-
70) and mitted light observation, using the IX-AN analyzer will prevent decreased
excitation light intensity and make observation of bright images possible.
of the
ersion
4. Rotate the condenser turret @) to select the “BF” brightfield observation
light path (no optical element in the light path) (Fig. 97).
5. To engage the polarizer into the light path, move the polarizer lever (8) on
the polarizer unit (IX-LWPO) to the right.
6. Engage the 10X objective and place a specimen suitable for brightfield
observation on the stage. After bringing the specimen into approximate
focus, remove the specimen from the light path.
NOLLVAH3ISEO ILSYYLNO9 39INIYI4YILNI IWILN34Y34410 &gt;
=
DIFFERENTIAL INTERFERENCE CONTRAST OBSERVATION E
&raquo;
N
Fig. 98
Fig. 99
7. When the binocular observation tube (U-BI9OCT) is used, rotate the CT
turret (9) to the “CT” position to engage the CT lens. (Fig. 98)
When using the binocular observation tube (U-BI90), or the trinocular
observation tube (U-TR30H), replace an eyepiece with the focusing
telescope (U-CT30).
8. Turn the focusing ring (0) {the knurled ring in case of the focusing telescope)
and bring the objective exit pupil into focus.
9. When the prism control knob (1 of the transmitted light differential
interference contrast slider (U-DICT) is rotated all the way in the clockwise
direction, the black fringe will be sandwiched between rainbow colored
fringes and should go diagonally through the center of the field. (Figs. 95,
99)
10. While looking at the objective exit pupil, rotate the polarizer slider polarizer
rotation/clamping knob (2 to the position where the black fringe is best
defined. (Figs. 97, 98)
&copy; The “crossed filter” position (total extinction) should be near the 0&deg; position
index.
11.0nce the position of the polarizer is determined, tighten the polarizer
rotation/clamping knob so that the polarizer does not rotate.
{ Observation )
1. Rotate the condenser turret @) to engage the Nomarski prism that matches
the objective to be used. (Fig. 97)
&copy; See Section 8, OPTICAL TURRET INSERTS AND COMPATIBLE OBJEC-
TIVES, for details on the various optical insert combinations.
2. Engage the objective to be used.
3. Place the specimen on the stage and move the stage to bring the specimen
into focus.
4. Adjust the field iris diaphragm until the diaphragm opening circumscribes
the field of view.
5. Stopping down the aperture iris diaphragm somewhat will increase the
contrast.
6. Rotate the prism contro! knob of the DIC prism slider to adjust the
interference color of the background, and to achieve maximum contrast
depending on the specimen under observation, as outlined below:
(1) Rotating the prism control knob of the slider will continuously change
the interference color of the background from gray to magenta (200
to 700 nm).
e |fthe background color is black (&cent;p-order fringe), darkfield like observa-
tion Is possible.
e |fthe background color is gray, a pseudo relief image with maximum
contrast with gray sensitivity can be obtained.
e |fthe background color is magenta, even a minor optical retardation can
be observed as a color change.
* Care should be taken to keep the specimen surface clean, as even
a small amount of contamination on the surface may show up due
to the exceptionally high sensitivity of the differential interference
contrast method.
(2) As differential interference contrast exhibits directional sensitivity,
rotate the specimen on the stage as required to obtain maximum
contrast.
* For details on simultaneous reflected light fluorescence and trans-
mitted light observation, refer to the instruction manual for the
reflected light fluorescence attachment.
Xx If plastic vessels are used, the interference contrast effect is not
obtainable due to the de-polarizing effect of plastics.
IX50/1X70
imple Polarized Light Observation )
AA a
в | Universal Condenser {IX-LWUCD) (Figs. 100, 101) |
1. Remove the dummy slider from the revolving nosepiece.
2. Align the index (1) of analyzer (U-ANT) with the groove (2) in the dummy
slider, and place it into the opening. (Fig. 100)
3. Invert the dummy slider with the mounted analyzer and insert it into the
slot under the revolving nosepiece. Insert as far as the click-stop.
4. Rotate the turret 3) to select the “BF” brightfield observation light path
95, N (no optical element in the light path). (Fig. 101)
izer Fig. 100 5. Toengage the polarizer into the light path, move the polarizer lever (4) on
jest the polarizer unit (IX-LWPO) to the right.
N 6. Engage the objective to be used.
tion . . ,
7. Loosen the polarizer rotation/clamping knob @&amp;) a little by rotating it
izer counterclockwise. Move it horizontally until the total extinction position
Is obtained.
8. When the position of the polarizer is determined, tighten the polarizer
hes rotation/clamping knob to clamp the polarizer.
9. Place the specimen on the stage and bring it into focus. Simple polarized
EC- light observation can now be performed.
10. Adjust the field iris diaphragm until the diaphragm opening circumscribes
the field of view.
nen
| 11. Stopping down the aperture iris diaphragm somewhat will increase the
bes : contrast.
the
the
rast =
m
x
ge &gt;
200 A
&gt;
Г
Na- &gt;
—
mM
um 2
m
os)
can г
О
jen o
Jue &gt;
nce 7
&gt;
0
&quot;ty, —
um o
0
Mm
ns- 2
the &gt;
—
O
not Z
A
IN
с
| 7-1 Still Photography | hn
&copy; Use a trinocular observation tube (U-TR30H), the side port, or the SLR port (microscope frame 1X70 only) for photo-
micrography.
Photomicrography can be performed using either the PM-10, the PM-20, or the PM-30 photomicrographic system
cameras. Procedures for operating the photomicrographic units are described in their respective instruction manuals.
| Procedures specific to this microscope are described below.
Attaching the Straight Photo Tube (IX-SPT) (Figs. 102, 103)
| Trinocular Observation Tube (U-TR30H) (Fig. 102) | *
1. Using the Allen screwdriver, loosen the clamping screw (1) on the
trinocular tube photo port and remove the cap.
2. Align the red index dot (2) with the red index dot (3) on the straight photo
tube (IX-SPT), then mount the straight photo tube on the trinocular tube
photo port.
3. Tighten the clamping screw Q).
| Microscope Frame (IX50/1X70) Side Port (Fig. 103) |
1. Using the Allen screwdriver, loosen the clamping screw (1) at the
microscope frame side port.
2. Align the index dot &copy; on the straight photo tube (IX-SPT) with the red
positioning dot @ on the side port, then attach the straight photo tube to
the side port. |
3. Tighten the clamping screw (7) firmly.
Photo Eyepiece (Fig. 104)
Use only a PE photo eyepiece for photomicrography. Insert the photo
eyepiece (1) of your choice into the straight photo tube.
&copy; The SLR port {microscope frame {X70} is equipped with a built-in 2.5X PE
= photo eyepiece.
Fig. 104
re
PHOTOMICROGRAPHY, TV OBSERVATION =
D
A
LD
Fig. 107
IX50/1X70
El Mounting the Camera Unit (Fig. 105)
* Place the camera unit directly over the circular dovetail of the straight photo
tube. Make sure the index dots e (1) on the straight photo tube and the
camera unit are aligned, then clamp the unit.
Mount to the side port in the same manner.
e For details on mounting the SC35 camera to the SLR port, refer to page 14.
a Selecting the Observation Tube Light Path
See page 29 of the “Observation Tube” section.
a Using the Photo Mask (Figs. 106, 107)
| Microscope Frame (1X70) |
Pull out the photo frame (1) to engage the photo mask into the light path
for observation. (Fig. 106)
&copy; If the photo frame is pulled further out than when placed in the light path
for observation, the photo mask slider can be removed from the
microscope frame, and dirt or other contaminants can be cleaned off.
When mounting the slider, do so with the inscription on the slider facing
towards the observer and the positioning pin fitting into the positioning
groove.
&copy; The cross-lines in the center of the photo mask are used for eyepiece
diopter adjustment.
&copy; The mask indicates the areas covered when using a 35mm film, and the
numerals next to the lines correspond to the magnification of the
respective PE photo eyepiece. (Fig. 107)
&copy; When using 4X 3&quot; film, the photographed area will be one step smaller
than the 35 mm film reticles.
&copy; When using the SLR port for photography, a 2.5X photo magnification
will be effective. Accordingly, use the 2.5 masks. The built-in 2.5X photo
eyepiece may be replaced with another of different magnification (4X,
5X).
For details, please consult your Olympus representative.
| Microscope Frame (1X50) |
This microscope frame is not equipped with a photo frame.
NOILVAJISdO AL “AHdYHIOHIINOLOHd N
A
Ol
PHOTOMICROGRAPHY, TV OBSERVATION N
A
O)
Fig. 108
a Focus Adjustment (Figs. 108, 109)
&copy; Focusing adjustment for photomicrography is performed by using the
built-in photo mask of the microscope frame (1X7), or the photo mask of the
camera unit's focusing telescope, or by using the photo mask of a finder
eyepiece 35WH10X.
* If no photo mask is used, the film plane may not be in focus, even
if the image is in focus in the binocular tube.
| Microscope Frame (1X70) (Figs. 108, 109) |
1.
Camera focusing 1s done using the built-in photo mask of the binocular
observation tube.
. The photo mask has a reticle with four masks and a double cross-line,
and the focus is for all purposes the same for the reticle and the camera
film plane. The masks indicate the areas covered, and the numerals next
to the masks correspond to the magnification of the photo eyepiece.
(Fig. 107)
Looking through the finder eyepiece, adjust the diopter ring so that the
double cross lines can be clearly observed as two distinctly separated
lines. (Fig. 108)
Using the coarse and fine adjustment knobs, bring the specimen into
focus. Since the focusing lens reticles and the film plane are in precise
alignment, bring the double cross-lines and the specimen into simulta-
neous focus using the coarse and fine adjustment knobs.
&copy; Because of the great depth of focus of 2.5X to 4X objectives, use an
optional focusing magnifier (U-FT) for accurate focusing.
&copy; Since it will be difficult to use the standard focusing telescope of the
camera unit when attached to the side port, it Is recommended to use
a V-shaped focusing telescope (U-FTV) ). (Fig. 109)
* When using a low power objective, the focusing may be lost when
the photo mask is pulled out after use. In this event, focus once
again.
Be sure to use the photo mask when adjusting the focus of the side
port.
* When the camera unit is attached to the trinocular observation
tube, do not use the built-in photo mask of the microscope frame.
Be sure to use the finder eyepiece 35WH10X or the photo mask of
the camera unit's focusing telescope. When the camera unit is
attached to the side port, adjust the focus by using the photo mask
of the camera unit's focusing telescope.
! How to Use the U-FTV |
e Turn the diopter adjustment ring of the focusing telescope to the +2 index
position.
* Slide the top portion of the U-FTV (1 up and down until the photo mask is
brought into focus. (Fig. 109)
(Photographic Magnification &raquo;
* 35 mm photo magnification
= Objective magnification X PE photo eyepiece X magnification changer
* Large format camera back magnification
= 35 mm photo magnification X 3
IX50/1X70
| Microscope Frame (IX50)
To adjust focus when using this microscope frame, attach a finder
eyepiece to the eyepiece portion of the trinocular observation tube, or
use the focusing telescope on the camera unit.
When the camera unit is attached to the side port, itis not possible to focus
using the 35WH10X. Use the camera unit's focusing telescope. If it is
difficult to use the focusing telescope, it is recommended to use a V-
shaped focusing telescope (U-FTV).
* Note thatthe finder eyepiece 35WH10X cannot be attached to the
binocular tubes U-B190CT, U-B190.
Adjusting the Illumination
Correct illumination is more crucial for photomicrography than for
observation since flawless pictures cannot be obtained unless the
illuminating light is properly adjusted. To avoid uneven illumination,
especially with high contrast film, adjust the illuminating light by carefully
following the observation procedures.
a Radiant Heat Influence from the Illumination
Even at optimum illumination setting for observation and photomicro-
graphy, the illuminating light will generate considerable radiant heat which
may damage living specimens. In case of interference contrast observa-
tion special caution should be exercised. To prevent this damage, consider
the following precautions:
* Reduce light intensity for observation as much as possible.
* Use additional heat absorbing filters for observation.
* For time-laps photography, synchronize the on/off of the light bulb with
the exposure (use SYNCHRONOUS mode).
au Filters and Lamp Intensity Setting
5 Set the lamp intensity and filters in accordance with the film to be used.
К - 0
Light intensity ая
Film Filter adjustment slider 9 |
Daylight type color film 45L BD-IF Camera symbol position =
x Tungsten type color film | 45LBT Camera symbol position Q
; Other position than camera &copy;
B &amp; W film 431F550-W45 | symbol position o
IS 5
* To match your preference in color rendition, make test exposures т
for determining the lamp intensity. &lt;
&copy; When IF550 is not used for color photography of phase contrast images, 2
a neutral color is obtained by combining LBD filters and setting the lamp О
er light intensity at around the lower limit of camera symbol. a
2
&gt;
—
O
2
&egrave;
IN
~
( 7-2 TV Observation |
&copy; The following five methods can be used for TV observation.
1. Attaching the video camera to the side port.... To obtain a bright video image with good resolution.
2. Attaching the video camera to the trinocular observation tube (U-TR30H).... To obtain a video image with a high magnification.
3. Attaching the video camera to the SLR port (IX70 only).
4. Attaching the video camera to the bottom port. (Requires modification) (IX70 only)
5. Attaching the video camera to a right hand side port. (Requires modification) (IX70 only).
Attaching the Video Adapter to the Side Port (Figs. 110, 111)
J
1. Loosen the side port clamping screw (1) using the Allen screwdriver.
2. Fit the video adapter (IX-TVAD) @ onto the side port and tighten the
clamping screw (1). (Fig. 110).
— | 5 3. Firmly screw the C/T-mount adapter (U-CMT) 3) or F/T-mount adapter
(U-FMT) (9) onto the video adapter (IX-TVAD) O). (Fig. 111)
4. Attach a video camera compatible with U-CMT or U-FMT.
| &gt; { Adjustment of observation image and monitor image Y
1. Adjust the eyepiece to bring the specimen into focus.
2. Lightly loosen the clamping screws (7, &reg;. (Figs. 110, 111)
3. While observing the monitor image and holding the U-CMT (6) or
U-FMT @) stationary, rotate IX-TVAD (2. Tighten the clamping screws (7)
and 6) when the image is in focus. (Figs. 110, 111)
* |f the clamping screw () is facing downwards and is difficult to
tighten, loosen the screw (1) and then hold only the IX-TVAD and
rotate it until the screw 6) faces upward. Then, tighten the screw
(5). Next, rotate to the original position and tighten the screw (1).
Attaching the Video Adapter to the Trinocular (Fig. 112)
Observation Tube (U-TR30H) is
. Attach the adapter in the same manner as outlined on page 44, “1.
Attaching Photographic Tube (IX-SPT).”
. Insert the PE photo eyepiece 2) into IX-SPT (1), and attach the video adapter
for the Olympus video system.
. Mount a video camera compatible with the video adapter. (Refer to the
Instruction manual provided with the video system.)
PHOTOMICROGRAPHY, TV OBSERVATION = [
A
Qo
&oacute;
IX50/1X70
Attaching the Video Adapter to the SLR Port (Figs. 113,114) |
| Microscope Frame (1X70) |
1. Using the Allen screwdriver, loosen the two clamping screws (1) holding
the intermediate body of the OM adapter on the SLR port. Remove the
front section (2) of the adapter. (Fig. 113)
2. The rear section of the adapter is provided with a thread (3) for mounting
a T-mount. Firmly screw a C/T-mount adapter (U-CMT) @) or an F/T-mount
adapter (U-FMT) onto the tread. (Fig. 114)
&copy; A commercially available T-ring (mfd. by Vixen) may also be used.
Attaching the Video Adapter to the Bottom Port
Modification is required to attach the video adapter to this port. For
details, please consult your Olympus representative.
Attaching the Video Adapter to the Right Hand Side Port
Modification is required to attach the video adapter to this port. For
details, please consult your Olympus representative.
ще
I
e
+
O
=
O
I
O
&copy;
2
&gt;
0
I
&lt;
О
o
N
m
x
&gt;
=
O
&lt;
A
&copy;
PHOTOMICROGRAPHY, TV OBSERVATION
OT
O
a TV Observation
1. Adjusting the microscope frame
Turn on the light source and perform all the required adjustments for
observation.
2. Adjusting the video camera/monitor
Perform white balance and color adjustments as required.
* The center of the eyepiece and the center of the monitor may not
match. However, this is a problem with the video camera's
mechanism for adjustment.
3. Simultaneous focusing of the observed image and the monitor image ’
In accordance with the adjustment method of each video adapter, adjust
for simultaneous focusing of the observed image and the monitor image.
{ TV Observation Magnification )
TV observation magnification
= Objective magnification
X Video adapter lens magnification
Video monitor size
Video camera format
IX50/1X70
Turret
Observation Turret insert ; iecti
Condenser method insert replace- Compatible objectives
ment
IX-PHL (S) O UPlanFI 4XPh
IX-PHC (S) O CPlan10XPh, LCAch20XPh, CPlanFI10XPh
IX-PH1 (S) O UPlanFITOXPh, UPlanFI20XPh, LCPlanFIZ20XPh,
Phase UPlanApo10XPh
contrast IX-PH2 (S) O UPlanFI4ZOXPh, LCPlanFI4OXPh, LCPlanFIGOXPh,
(Note 1) UPlanApo20XPh
LCAch40XPh
IX-PH3 (L) O UPlanFITOOXOPh, UPlanApo40XOI Ph,
IX-LWUCD UPlanApo100XOI Ph, PlanApo60XOPh
IX-DP10 (5) O UPlanFI10XUPlanApo10X
interfer- IX-DP40 (L) O LCPlanF140X, UPlanApo40X
ence IX-DP60 (L) O LCPlanFIGOX
contrast IX-DPO60 (L) O UPlanApo60XWPSF
IX-DP100 (L) O UPlanFI100Xo, UPlanApo100Xo
Brightfield — — NA 0.13 — 0.9 objectives (Note 2)
PHL (built-in) X UPlanFI4XPh
IX-PHCU O CPlan10XPh, LCAch20XPh, CPlanFI10XPh
Phase IX-PH1U O UPlanFI10XPh, UPlanFI20XPh, LCPlanFI20XPh,
contrast | UPlanApo.10XPh
PH2 (built-in X UPlanFI40XPh, LCPlanFI40XPh, LCPlanFI60XPh,
IXULWED UPlanApo20XPh,
LCAch40XPh
af: — Magnification 2.5X or more and NA 0.75
Brightfield or lower objectives
Phase PHL (built-in) X UPlanFI4XPh
contrast PHC (built-in) X CPlan10XPh, LCAch20XPh, CPlanFI10XPh
IX-SLWCD
Brightfield _ Magnification 2.5X or more and NA 0.3
or lower objectives
Note 1: Small diameter turret inserts (5) should be placed in the 30 mm diameter turret opening, and large diameter turret
inserts (L) should be placed in the 38 mm diameter turret opening.
Note 2: When combined with an objective of NA 0.3 or higher, the resolution may decrease somewhat. For a well
observation, the phase contrast effect can be obtained in a large area of the field of view when the PHC is used.
В SIALLIIrgo 319ILYdNOJ ONV SLYISNI LIYYHNL
SPECIFICATIONS o
O1
N
item Specifications
IX70-S8F2 3-step light path selector (shockless design): C1 S8F2: Side port 80%
IX70-S1F2 S1F2: Side port 100%
SLR port (single-lens reflex), built-in intermediate mag. (1X, 1,5X) lens, built-in
photo mask, sextuple revolving nosepiece, aberration corrected 1X image (side
Microscope port), revolving nosepiece vertical stroke: 7 mm upward, 2 mm downward, with
frame reduction gear, graduated in increments of 1p.
IX50-S8F 2-step light path selector (shockless design): UT S8F: Side port 80%
IX50-S1F S1F: Side port 100%
No SLR port, no built-in intermediate mag. lens, sextuple revolving nosepiece,
aberration corrected 1X image (side port), revolving nosepiece vertical stroke:
7 mm upward, 2 mm downward, with reduction gear, graduated in increments of 1p.
IX-ILL100LH Column with lamp housing, 30&deg; inclinable mechanism, tilting condenser holder,
[llumination 100W halogen bulb, pre-centered.
column
IX-1LL30 30W halogen column, for use with condenser IX-ULWCD or IX-SLWCD.
U-BISOCT Binocular tube, 45&deg; tube inclination, built-in CT, one eyepiece sleeve with diopter
adjustment, 2-step eyepoint: 400 mm or 430 mm
Observation U-B190 Binocular tube, 45” tube inclination, one eyepiece sleeve with diopter adjustment,
tube 2-step eyepoint: 400 mm or 430 mM
U-TR30H Trinocular tube, 30&deg; tube inclination, mounted by means of intermediate tube
IX-ATU.
IX-LWUCD NA 0.55, WD 23 mm, 5-aperture turret
Condenser IX-ULWCD NA 0.3, WD 73 mm, 4-aperture turret
U-UCDB Dry: NA 0.9, WD 1.5 mm
Oil: NA 1.4, WD 0.63 mm
IX-SFR Right low positioned coaxial knobs, flexible stalk, movement range:
50 mm x 50 mm; stage insert plate diameter: 110 mm
Stage IX-SVL Left low positioned coaxial knobs, movement range:
9 43 mm (Y) x 50 mm (X); stage insert plate diameter: 110 mm
IX-SP/IX-MVR | Size: 240 mm (Y) X 232 mm (X); stage insert plate diameter: 110 mm; movement
range: 87 mm {Y) x 132 mm (X); low positioned coaxial knobs, flexible stalk.
WH10X Fieid number 22
Eyepiece WH10X-H Field number 22, with diopter adjustment helicoid.
35WH10X Field number 22, with photo mask and diopter adjustment helicoid.
item Specifications
CPlan10XPh NA 0.25, WD 9.8 mm, ring slit IX-PHC, PHCU
Objective Achromatic CPlanFI10XPh NA 0.3, WD 9.5 mm, ring slit IX-PHC, PHCU
. phase contrast LCAch20XPh NA 0.4, WD 3 mm, ring slit IX-PHC, PHCU
LCAch40XPh NA 0.55, WD 1.2 mm, ring slit IX-PH2
IX50/IX70
item Specifications
UPlanFI4XPh NA 0.13, WD 17 mm, ring slit IX-PHL
UPlanFI10XPh NA 0.3, WD 10 mm, ring slit IX-PH1, PHCU
Plan semi LCPlanFI20XPh NA 0.4, WD 6.9* mm, ring slit IX-PH1, PH1U,
apochromat correction cap system
phase contrast LCPlanFI40XPh NA 0.6, WD 2.6* mm, ring slit IX-PH2, correction
cap system with correction collar
LCPlanFI6OXPh NA 0.7, WD 1.7* mm, ring shit 1X-PH2, correction
cap system with correction collar
UPlanF110X NA 0.3, WD 10 mm
Plan semi LCPlanF120X NA 0.4, WD 6.9* mm, correction cap system
apochromat LCPlanF140X NA 0.6, WD 2.6* mm, correction cap system
DIC brightfield with correction collar
Objective LCPlanFI60X NA 0.7, WD 1.7* mm, correction cap system
with correction coilar
Brightfield Plan Apos0XO NA 1.4, WD 0.1 mm
high resolution UPlanF!100XO NA 1.3, WD 0.1 mm
DIC UPlanApo100XO1 | NA 1.35, WD 0.1 mm, with iris diaphragm
UPlanApo10X NA 0.4, WD 3.1 mm
seed font UApo20X/340 NA 0.75, WD 0.55 mm, with water-proof cap
fluorescence UApo40X/340 NA 0.9, WD 0.2 mm, with water-proof cap
Fura-2 UApo40X01/340 NA 1.35, WD 0.1 mm, with iris diaphragm and
water-proof cap
Water NA 1.2, WD 0.25 mm, correction collar range:
immersion UPlanApo60Xw
objective 0.15-0.19 mm
*When the correction cap for standard culture vessel is used.
Brightness adjustment range: DC 1.0-11.9V (continuous), with light-off switch
Power requirements: 100-120V/220-240V &quot;v 1.8A/0.8A 50/60 Hz
[X70-S8F2 Rated output: 12V 100W
IX70-S1F2 Bulb used: 12V 100W Halogen lamp PHILIPS 7724
Average life time of bulb: approx. 2,000 hr.
Applicable fuse: += T5A(H) 250V (LITTEL FUSE 215005)
Brightness adjustment range: DC 1.0 -5.9V (continuous)
Power requirements: 100-120V/220-240V NL 0.8A/0.4A 50/60 Hz
Main IX50-58F2 Rated output: 6V 30W
Frame IX50-51F2 Bulb used: 6V 30W Halogen lamp PHILIPS 5761
Electrical Average life time of bulb: approx. 100 hr.
System Applicable fuse: == T3.15A(H) 250V (LITTEL FUSE 2153.15)
e Indoor use
e Altitude up to 2000 m
e Temperature: 5&deg; to 40&deg;C (41&deg; to 104&deg;F)
Operation * Maximum relative humidity 80% for temperatures up to 31&deg;C (88&deg;F)
environment | decreasing linearly to 50% relative humidity at 40&deg;C (104&deg;F)
e Main supply voltage fluctuations not to exceed = 10% of the nominal voltage
o Installation/Overvoltage Category II {In accordance with IEC664)
e Pollution Degree 2 (In accordance with |EC664)
SNOILVI4131dS
53
|
|
|
|
на &lt;
В TROUBLESHOOTING GUIDE
OT
A
Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If
problems occur, please review the following list and take remedial action as appropriate. If you cannot solve the
problem after checking the entire list, please contact your local Olympus representative for assistance.
Problem Cause Remedy Page
1. Bulb does not light. Light-off switch is set to ON. (1X70 only) Set light-off switch to OFF. (IX70 only) 22
Bulb burned out. Replace bulb. 4,5
Fuse burned out. Replace fuse. 15
2. Bulb lights, but field of Line voltage selector switch is not set to | Set the switch to the position matching the
view remains dark conform with local mains voltage. local line voltage (100-120V or 220-240V). 15
(IX50 only) (1X50 only)
Light intensity lever is in low position. Set lever to appropriate position. 22
Condenser is not in correct position. Adjust the condenser height until the field iris
diaphragm image is formed in the specimen 34
plane.
Condenser is not centered. Center the condenser until the field iris
diaphragm image is centered in the field of | 34
view.
The revolving nosepiece is not correctly en- Make sure that the revolving nosepiece clicks | —
gaged. properly into place.
The light path selector knobis setto SLR port Set the knob to binocular eyepieces or raise 23
or side port. the lamp voltage.
Too many filters are engaged. Reduced the number of filters to the mini- | 32
mum required,
The stage insert plate blocks the light path. Move the stage and place the specimen | 25
again.
Field iris diaphragm is not opened wide | Open the field iris diaphragm sufficiently. 33
enough.
Trinocular tube light path selector knob is set Set the knob at intermediate or pushed-in 29
to pulled-out position. position.
The U-BI9OCT turret position is “S”. Rotate the turret to the “0&quot; position 30
3. Field of view is obscured, or | Trinocular tube light path selector knob is in Fully engage the knob at the click-stop | 29
field of view is not evenly | middie position. corresponding to the observation method.
illuminated IN
An objective that falls outside of the Use a condenser that matches the objective. 51
condenser's illumination range is used.
The field iris diaphragm is not properly | Center the field iris diaphragm correctly. 34
centered.
The field iris diaphragm is stopped down too far. Open the field iris diaphragm sufficiently. 33
Light path selector knob on the frame is in Fully engage the knob at the click-stop | 29
middle position. corresponding to the observation method.
Magnification selector knob (1X70 only) is in Fully engage the knob at the click-stop | 23
middle position. corresponding to the observation method.
|
The revolving nosepiece or magnification Fully engage the knob at the click-stop. 23
selector knob (IX70 only) is in middie position.
The condenser turretis not correctly engaged. Engage the condenser turret at a click-stop. —
Filter is not engaged correctly. Set filter at appropriate position. —
Frosted filter is not inserted. Insert the frosted filter. 32
4. Dirt or dust is visible Dirt/dust on specimen, Clean thoroughly.
in the field of view.
Dirt/dust on eyepiece.
Dirt on photo mask. —
Dirt on mirror cube.
Dirt on turret insert.
Condenser is not correctly positioned and | Adjust the condenser height until the field iris
the frosted filter, etc. is in focus. diaphragm image is formed in the specimen 34
plane.
——e
Problem Cause Remedy Page
5. The image shows Condenser is raised too high. Lower to the proper position. 34
diffraction
The aperture iris diaphragm is stopped down | Open the aperture iris diaphragm. 37
too far.
6. Visibility is poor. You are using a non-UIS series objective. Use only UIS series objectives with this mi- | 52,53
* Image is not sharp. croscope.
: contrast IS E distinot The correction collar on the correction collar | While focusing, turn the correction collar to 39
equipped objective 1s not adjusted. find the best position.
Front lens of the objective is dirty. Clean the objective. —
Immersion oil is not being used with an oil | Use immersion oil. 40
Immersion objective.
The immersion oil contains bubbles. Remove bubbles. —
Inappropriate slide or cover glass thickness. Replace with glass of appropriate thickness. —
Dirty condenser, objective, eyepiece, culture | Clean thoroughly. —
vessel, etc.
Ring attachment and phase plate are not | Center correctly. 36
centered.
Poor contrast during differential interference | Replace plastic culture vessel with glass —
contrast observation. vessel.
Culture vessel bottom thickness and the | Replace with suitable correction cap.
respective objective correction cap type do 39
not match.
7. Part of the image is The objective is not correctly engaged in the | Make sure that the revolving nosepiece clicks —
blurred. light path. Into place correctly.
The specimen is not placed flat on the stage. | Place the specimen correctly on top of the | 6,7
stage and secure it with the specimen holder.
8. The coarse and fine ad- The tension adjustment ring is tightened | Loosen the ring. 24
justment knobs are hard excessively.
to turn.
Youare trying to raise the revolving nosepiece | Unlock the prefocusing lever. 24
with the coarse adjustment knob even though
the prefocusing lever is locked.
9. The revolving nosepiece
drifts down by itself, or . Lo
focus is lost during ob- The tension adjustment ring is too loose. Tighten the ring. 24
servation.
10. Coarse adjustment will Prefocusing lever is keeping the revolving | Unlock the lever. 24
not go all the way up. nosepiece down.
11. Field of view of one The interpupillary distance is incorrect. Adjust the interpupillary distance. 28
eye does not match
that of the other. Incorrect diopter adjustment. Adjust the diopter setting. 28,29
The optical axes are not parallel. Upon looking into the eyepieces, try looking
at the overall field before concentrating on
the specimen range. You may also find it | —
helpful to look up and into the distance for a
moment before looking back into the
Microscope.
This device complies with the requirements of both directive 89/336/EEC
concerning electromagnetic compatibility and directive 73/23/EEC concerning
low voltage. The CE marking indicates compliance with the above directives.
IX50/1X70
—
x
O
с
o
m
wn
I
O
O
—
zZ
&copy;
&copy;
C
o
ГП
errr YN dans mms
Bl PROPER SELECTION OF THE POWER SUPPLY CORD
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to
“Specifications” and “Certified Cord” below:
CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer
warrant the electrical safety of the equipment.
Specifications
Voltage Rating
Current Rating
Length
Temperature Rating
Fittings Configuration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
6A minimum
60&deg;C minimum
3.05 M maximum
Grounding type attachment plug cap. Opposite terminates in molded-on
IEC configuration appliance coupling.
Table 1 Certified Cord
A power supply cord should be certified by one of the agencies listed in Table 1, or comprised of cordage marked
with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of
agencies listed in Table 1. In case you are unable to buy locally in your country the power supply cord which is
approved by one of the agencies mentioned in Table 1, please use replacements approved by any other
equivalent and authorized agencies in your country.
Country Agency Certification Country Agency Certification
Australia. SAA XY Italy IMQ &copy;
Austria &Ouml;VE Gv Japan MIT) NA
Belgium CEBEC Netherlands | KEMA
Canada CSA | G. Norway NEMKO (N)
Denmark DEMKO &copy;) Spain AEE
Finland FE! (FD Sweden SEMKO (5)
France UTE Switzerland SEV &copy;
— e E SO
ireland NSAI &lt;&gt; USA. UL UL
56
—— — —_——
___IX50/1X70
Table 2 HAR Flexible Cord
APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Printed or embossed Harmo- epa Marking Tang
| izati nization Marking (May be o
Approval Organization located on jacket or insulation (Length of color section in mm)
of internal wiring) Black Red Yellow
| trotechni Bel
ES rotechnique Belge CEVEC &lt;HAR&gt; 10 30 10
Verband Deutscher Elektrotechniker
(VDE) e.V. Pr&uuml;fstelle &lt;VDE&gt; &lt;HAR&gt; 30 10 10
on Technique de d'Electricite USE &lt;HAR&gt; 30 30 10
Instituto Italiano del Marchio di
Qualita’ {IMQ) IEMMEQU &lt;HAR&gt; 10 30 50
British O Service for Electric BASEC &lt;HAR&gt; 10 10 30
N.V. KEMA KEMA-KEUR &lt;HAR&gt; 10 30 30
SEMKO AB Svenska Elektriska SEMKO &lt;HAR&gt; 10 10 50
Materielkontrollanstalter
Osterreichischer Verband f&uuml;r :
Elektrotechnik (ОМК) &lt;OVE&gt; &lt;HAR&gt; 30 10 50
|
ers Elektriske Materielkontrol &iquest;DEMKO&gt; &lt;HAR&gt; 30 10 30
o NSAN OS Authority of &lt;NSAI&gt; &lt;HAR&gt; 30 30 50
Norges Elektriske Materiellkontroll NEMKO &lt;HAR&gt; 10 10 70
(NEMKO)
Asociacion Electrotecnica Y
R
Electronica Espanola (AEE) &lt;UNDE&gt; &lt;HAR&gt; 30 10 70
Hellen organization for Standardiza- ELOT &lt;HAR&gt; 30 30 70
Oe Portugues da Qualidade | np | &lt;HAR&gt; 10 10 90
Schweizerischer Elektro Technischer
Verein (SEV) SEV &lt;HAR&gt; 10 30 90
Elektriska Inspektoratet SETI &lt;HAR&gt; 10 30 90
Underwriters Laboratories Inc. (UL)
Canadian Standards Association (CSA)
SV, SVT, SJ or SIT, 3 X 1BAWG
SY, SVT, SJ or SJT, 3X 18AWG
же еее ее неее ева то +5
+ APR
т . &quot;
ar
OLYMPUS
OLYMPUS OPTICAL CO. LTD.
43-2, Hatagaya 2-ch&ocirc;me, Shibuya-ku, Tokyo Japan
OLYMPUS OPTICAL CO. (EUROPA) GMBH
(Premises/Goods delivery) Wendenstrasse 14-16, D-20097 Hamburg, Germany
(Letters) Postfach 10 49 08, 20034 Hamburg, Germany
OLYMPUS AMERICA INC.
2 Corporate Center Drive, Melville, N.Y. 11747-3157, U.S.A.
OLYMPUS OPTICAL CO. (U.K.) LTD.
2-8 Honduras Street, London EC1Y OTX, United Kingdom
This publication is printed on recycled paper.
Printed in Japan 9807 M 045—-0
">
Witaj! Jestem chatbotem AI specjalnie przeszkolonym, aby pomóc Ci w obsłudze Olympus IX 50. Dokładnie przejrzałem dokument i mogę pomóc Ci zlokalizować dokładne informacje, których potrzebujesz, lub wyjaśnić treść w jasny i prosty sposób. Niezależnie od tego, czy szukasz wskazówek dotyczących konkretnych funkcji, kroków rozwiązywania problemów, czy ogólnego użytkowania, możesz zadawać pytania. Im więcej szczegółów podasz na temat swoich obaw lub potrzeb, tym dokładniej i skuteczniej mogę Ci pomóc!
Asystent pisze
Asystent jest niedostępny. Spróbuj ponownie później.
Wygląda na to, że dotarłeś do dokumentu napisanego w wielu językach, w tym w języku, który nie jest Twoim językiem ojczystym. Możesz użyć poniższych linków, aby tymczasowo ukryć strony bez preferowanego języka.