ECLIPSE TE2000-S, TE2000-U, TE2000-E, TE2000-PFS are advanced inverted research microscopes developed by Nikon for live-cell imaging and cutting-edge research. The TE2000 series is designed to provide accurate focus for long-term observations and high S/N ratio images. The TE2000-PFS (Perfect Focus System) provides automatic focus correction and is ideal for time-lapse recording and live-cell imaging, including TIRF.
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Inverted Research Microscope
Inverted Research Microscope TE2000
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The TE2000-S, a basic model that can be dedicated to specific tasks, comes with two output ports.
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The TE2000-U is a universal model that comes standard with four output ports.
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The TE2000-E incorporates a highprecision motorized focus and vibration-free motorized optical-path changeover mechanism that facilitates image capture in 3D. The TE2000-E comes with five output ports.
By combining the fully motorized TE2000-E with the Nikon Perfect Focus System (PFS), the
TE2000-PFS guarantees constant and accurate focus, making this latest model perfect for live-cell imaging, including TIRF and long term, dynamic time-lapse observations.
Configured with an epi-fluorescence attachment
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Focus drift is one of the biggest obstacles in time-lapse observation.
The new TE2000-PFS (Perfect Focus System) automatically detects the surface of the coverslip optically and continually corrects focus to compensate for even the most infinitesimal changes. Focus is maintained during long term observations and stage movement. Moreover, the TE2000-PFS includes all the functions of the acclaimed TE2000-E inverted research microscope.
• You can continue stable, in-focus observations over an extended period of time—perfect for time-lapse recording.
• You can minimize photobleaching, which keeps cells alive longer in fluorescence observation, through an overall reduction in the number of images captured.
• You will never again miss sudden changes in your specimen as PFS instantaneously corrects focus drift or
Z-axis changes resulting from temperature drop when adding reagents.
• You can freely select focus planes throughout the specimen and keep focusing on the selected plane thanks to the Simultaneous Optical Offset feature.
Nikon Original!
When PFS is turned on, the position of the coverslip surface is always detected during observation. The data is continuously fed back to the extremely accurate Z-axis control focusing mechanism thanks to Nikon’s propriety COF (Continuous
Optical Feedback) technology*. Focusing precision of less than
1/3 the focal depth of the objective is maintained.
*Patent pending
•PFS on
Configured with an epi-fluorescence attachment
PFS can also work in brightfield, phase, and DIC observations.
Principle of focus detection
Medium; n: approx 1.35
Glass; n: approx 1.5
Water: n: 1.333
Oil; n: 1.514 or Air; n: 1.0
LED detection light
The coverslip surface* is detected by the LED light emitted through the objective.
*Interface of glass and medium in immersion applications or glass and air in dry applications.
▲
Adding reagent
Correction to focus drift caused by expansion/contraction of the plastic dish when reagents are added
Observation method: Laser TIRF
•PFS off
▲
Adding reagent
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Nikon Original!
Focus is continuously corrected at any plane of interest throughout the specimen by the Simultaneous Optical Offset feature*. Unlike other systems that have to repeat focusing on the coverslip surface and then the plane of interest in alternate shifts, PFS can maintain constant focus on the plane of interest at millisecond refresh speed. Consequently, you will never again miss rapid events in your specimen because of focus drift.
*Patent pending
Focus is maintained during time-lapse recording
PFS uses an LED emitting light in the infrared range and an internal linear CCD detector to detect the precise interface, so it does not intrude on the visible wavelengths used for fluorescence emission. This means you can carry out observation and focus control at the same time, with no influence at all on captured images. Single fluorescent molecules can also be visualized at a high S/N ratio.
Single molecular fluorescence image of YFP label receptor
Observation method: DIC
3
µ m
Contours of the cell photobleach
0 0.1
0.2
0.3 (s)
Cell: dendrite (part) of a primary dispersion culture cell of a hippocampus
Time-lapse image: being photobleached at after 0.3 s
Observation method: TIRF
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The TE2000-E comes standard with linear encoded motorizedfocus and motorized 5-way light port changeover—perfect for advanced research that requires high resolution image capture in
3D, including confocal microscopy and deconvolution processing.
Streamlined operation from a PC
The microscope can be operated from a
PC using Nikon’s NIS-Elements acquisition and analysis software or other third-party application software.
Greater Z-axis precision
The E model features an integrated Z-axis linear encoded readout of 0.05µm when controlled through a connected computer.
Objective anti-collision mechanism
The nosepiece automatically drops when the objectives are being changed, preventing them from hitting the stage— particularly useful for live cell observations.
TE2000-E configured with confocal system
Auto switching between 5 ports
Five output ports, including a bottom port, are standard and can be easily switched via motorized control.
External fine focusing unit
Fine focusing can be easily controlled anywhere on the desktop with this compact unit.
Remote control unit
All motorized units can be operated easily in a darkroom thanks to the phosphorescent display tags.
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Taking advantage of infinity optics, the TE2000’s stratum structure enables the extension of the distance between the microscope body and objectives by up to 80mm (max.).
This design allows the introduction of equipment such as laser tweezers or extra illumination into the optical path without modifying the microscope body as well as a variety of Nikon’s fluorescence illumination accessories.
Nikon’s “stratum structure” can efficiently provide a perfect system for your desired application. Multimode imaging capability is realized with a single microscope.
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Epi-fl attachment
Other attachment
Optional stage risers (70mm) allow the mounting of other equipment such as laser tweezers or a laser unit in addition to an epi-fluorescent attachment without modifying the microscope body.
Epi-fl attachment
Standard position
70mm
Epi-fl attachment
Other attachment
Stage risers
Multimode imaging as shown below is possible.
C1plus or C1si confocal laser scanning microscope system
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TIRF (Total Internal Reflection
Fluorescence)/epi-fluorescence system
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Laser tweezers
The C1confocal microscope system can be paired with a combination of epi-fluorescence and TIRF units. The confocal microscope system and TIRF unit share one laser light source, and allow you to observe TIRF and confocal images of the same specimen with one microscope. Utilizing the stratum structure, it is possible to add laser tweezers in another stratum or level and provide simultaneous observation with either TIRF, confocal or epi-fluorescence excitation.
Specimen
Coverglass
Objective
(CFI Apo TIRF 60X/1.49 oil)
Top Position
Laser Tweezers
DM (400,
505, 565)
Laser
(1064nm)
DM (IR reflection, visible transmitted)
Bottom Position
TIRF/Epi-fl Attachment
Beam splitter
Fiber
Mercury Lamp
C1 confocal scanning head
Laser
(488nm, 532nm)
Detector
Prism
Eyepiece Tube, Camera Port
Epi-fl illumination
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Near-IR-DIC attachment /FRET system
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CCTV camera
Utilizing the stratum structure, it is possible to mount an epi-fl illumination system and a near-infrared DIC attachment. The image can be observed under epi-fl illumination simultaneously with viewing of dynamics of living cells with near-IR DIC illumination as the image can be divided into two wavelengths by a dichroic mirror inside the filter turret of the near-IR DIC attachment.
In addition, FRET images can be captured with a spectrophotometer attachment.
Furthermore, simultaneous photometry of multiple fluorescence wavelengths with multiphoton excitation is possible.
Prism
IR Filter
Specimen
Coverglass
Halogen
Lamp
Objective
(CFI Plan Apo VC 60X WI)
Top Position
Epi-fl Illumination System
High-speed
Spectroscopic
Fiber Illuminator
DM (400, 505, 565)
Bottom Position
Near-IR DIC Attachment
DM ( = 780nm reflection, visible transmitted)
Dual-Wavelength
Spectrophotometer
IR CCD Camera for
Edge Detection
Eyepiece Tube, Camera Port
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Responding to an increasing demand for fluorescence observation of higher S/N ratio images, Nikon created a new mechanism, Noise Terminator. The TE2000 incorporates this mechanism in the fluorescence illuminator and filter cubes to totally absorb stray light in the optical path. This significantly reduces noise and dramatically increases fluorescence image contrast.
Lenses
Light absorbing material
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Light source
Stray light
Fluorescence filter cube
Nikon’s two process mechanism first removes stray light from the filter cube completely, then absorbs it through the light absorbing material.
Therefore stray light is eliminated thoroughly.
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Each filter/mirror incorporated in these filters has a very sharp rising edge at the corresponding wavelength. By minimizing the crossover of each signal, Nikon succeeded in significantly reducing the loss of excitation wavelength. As a result these filters can provide high quality epifluorescence images. There are three types of filters: CFP, GFP, and YFP.
By changing the material structure, Nikon succeeded in significantly improving the functionality of the standard combination of DIC modules and sliders.
The excellent balance of contrast and resolution produces high quality DIC images with no color blur at any magnification.
Depending upon the type of specimen, either a high-contrast or high-resolution combination is selectable.
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The TE2000 utilizes Nikon’s world renowned CFI60 infinity optics, known for crisp and clear images at any magnification, while providing higher NAs and longer working distances. The focal length of the tube lens is ideal at 200mm, which avoids induced aberration even when you introduce phase rings, DIC prisms or dichroic mirrors into the optical path.
CFI Plan Apochromat VC Series
• Chromatic aberrations have been thoroughly correct throughout the view field. Suitable for digital imaging.
• Perfect choice for multi-stained, fluorescence specimens and when using brightfield and DIC techniques.
• Axial chromatic aberration has been corrected up to the violet range (405nm), making these objectives highly effective for confocal applications.
• Excellent brightness throughout the view field.
• The 60X water-immersion type, in particular, features high spectral transmittance, even in the 360nm wavelength range.
CFI Plan Apochromat VC 60X Oil, NA 1.40
CFI Plan Apochromat VC 60X Wl, NA 1.20
CFI Plan Apochromat VC 100X Oil, NA 1.40
CFI Apochromat TIRF Series
CFI Apochromat TIRF 60X Oil, NA 1.49 w/correction collar
CFI Apochromat TIRF 100X Oil, NA 1.49 w/correction collar
• The unprecedentedly high NA of 1.49 enables excitation on an even thinner field to produce high S/N ratio TIRF images.
• The world’s-first temperature-change spherical aberration correction ring is provided for in the objective design. Users can easily counteract the influences to the image quality from temperature-induced changes—from 23ºC (room temperature) to 37ºC (physical temperature)—in the refractive index of the immersion oil.
• The correction ring works perfectly in both DIC and epifluorescence microscopy of minute structures. It is also suitable for the laser tweezer method.
• To be used with a regular coverglass and immersion oil.
Intensity distribution of dot image
23ºC 37ºC before correction
When the temperature changes, aberration occurs.
37ºC after correction
Aberration can be corrected using the correction ring.
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The TE2000-PFS and TE2000-E have 5 ports. The TE2000-U has 4 ports.
The TE2000-S has 2 ports. These ports distribute light in the ratios given below. The TE2000 can capture or analyze images with multiple cameras.
Main body
1 2
Port select address
3 4 5
TE2000-PFS Eye 100% Front 80% Bottom Right 80% Left 100%
TE2000-E
TE2000-U
Eye 20% 100%*
1
Eye 20%
Eye 100% Front 80% (Optional) Right 80% Left 100%
TE2000-S Eye 100%
Eye 20%
Left 80%
Eye 20%*
3
*
2
–
Eye 20%
– –
By changing the optional prism, the light distribution ratio of the shaded portions can be changed as below.
*1 Right: 100 Front: 100 Left: 80 (Eye: 20)
*2 Right: 100 Front: 100 Left: 80 (Eye: 20)
*3 Left: 100
The following portions can also be changed by altering the microscope body.
(Optional at the time of purchase)
*2 Bottom: 100
*3 Right: 100
Magnifications of all ports of the TE2000-PFS, TE2000-E and TE2000-U, including the observation port, can be easily changed without the troublesome adjustment accompanying the change of objectives.
TE2000-U/E/PFS
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To achieve stability that supports focusing precision, Nikon implemented Computer Assisted Engineering (CAE) and adopted a new high-strength alloy material in the microscope body. This doubled the rigidity compared with previous models.
To minimize focus deviation due to temperature change, Nikon has reinforced the thermal stability of the microscope body, thus improving image quality during long hours of observation or photography.
Glass stage ring
This glass stage ring ensures minimum deformation caused by temperature change, minimizing blurred focus; so it is suitable for time lapse imaging.
Nikon has instituted numerous innovations to provide comfortable operation and reduce strain, even over a prolonged period of operation.
Nosepiece is inclined to the left for easy handling.
Eye-level riser (as indicated by arrow)
Frequently used buttons and controls are all located at the front and within easy reach.
The TE2000-PFS and TE2000-E come with a compact external fine focusing unit that can be placed anywhere on the desktop.
The nosepiece is inclined to the left, making it easy to read the magnification and adjust the correction ring.
The low-profile stage facilitates handling of specimens.
Main controls are concentrated in the front, close to the operator.
External fine focusing unit
The 25º -inclination eyepiece tube minimizes fatigue during long hours of observation, while its Y-shaped design permits easy viewing of the specimen area on the stage.
An ergonomic tilting eyepiece tube is optionally available.
Furnished with a built-in Bertrand lens, the inclination angle is adjustable from 15º to 45º for viewing in a relaxed and comfortable posture.
Ergonomic tube
Optimal eyepiece height can be achieved by using optional eye-level risers. Each riser has a thickness of 25mm and up to two risers can be installed at a time. (The eye-level riser cannot be used with a stage riser)
Attaching the optional large stage-handle knob enables precise operation for the fine movement of the stage during high magnification observations.
Large stage-handle knob
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All four models accept retrofittable motorized accessories, allowing researchers to not only choose the desired combinations, but also to control the microscope from external PCs.
• A wide variety of motorized accessories are available and can be controlled by an external computer when a HUB controller is attached.
• Motorized switching of objectives and observation methods between epi-fluorescence, Nomarski DIC, phase contrast,
Hoffman Modulation Contrast is possible*.
*There are some restrictions depending on the combination of model and observation method.
Motorized barrier filter wheel
This motorized barrier filter wheel with a turret rotary system can mount up to 8 ø25mm barrier filters.
Motorized system condenser turret
This motorized turret for system condensers enables easier switching between brightfield, phase contrast,
Nomarski DIC, and Hoffman modulation contrast observations.
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Motorized sextuple DIC nosepiece
This nosepiece can be used for all observations including DIC. When the magnifications are being changed, the nosepiece automatically descends for easy, safe rotation of the objectives and then returns to the original height after rotation (only in combination with the TE2000-PFS or
TE2000-E model).
Motorized epi-fl filter rotating turret
A maximum of 6 epi-fl filter cubes can be mounted and easily changed with a remote control unit, even in dark rooms.
Communication HUB controller
The HUB controller manages all the cables of each motorized unit at the back of the microscope. By connecting to the RS-232C interface of a PC, motorized units can be controlled from the PC.
Epi-fl attachment with motorized shutter
This epi-fluorescence attachment has a built-in motorized illumination shutter.
Motorized excitation filter wheel
This motorized excitation filter wheel with a turret rotary system can mount up to 8 ø25mm excitation filters.
Motorized analyzer
This motorized analyzer is used to remove and insert an analyzer for
Nomarski DIC.
Remote control unit
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1
2
6
9
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5
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All motorized units attached to the microscope can be operated from this control pad with an LCD screen. Filter position labels glow in the dark for easy identification.
1. LCD Display
2. Objectives Changeover
3. Shutter Open/Close
4. Excitation Filter Changeover
5. Barrier Filter Changeover
6. Fluorescence Filter Block Changeover
7. Z-axis Reset
8. External Signal Output
9. LCD Operation Mode Changeover
10. LCD Backlight On/Off
11. LCD Brightness Control
12. Diascopic Lamp Control by Remote Pad
13. Diascopic Lamp On/Off
14. Diascopic Lamp Brightness Control
15. Condenser Cassette Changeover
16. Analyzer In/Out
17. Light Path Changing Prism Changeover
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Flawless, high-contrast fluorescence images
Thanks to a noise terminator mechanism and zoom illumination, the TE2000 achieves an unparalleled
S/N ratio and brightness. It performs well even for weak, single-molecular-level fluorescence observations at leading-edge research.
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The Noise Terminator mechanism directs deviated stray light out of the optical path. This results in images of high contrast and unparalleled S/N ratio.
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High quality fluorescence filters
Each filter/mirror incorporated in these filters has a very sharp rising edge at the corresponding wavelength that minimizes the crossover of signals.
Zoom-type lamphouse adapter allows the operator to increase light intensity.
Remote control unit and filter turret use phosphorescent display tags to enhance visibility during operation in dark rooms.
With the new DIC system, it is possible to obtain the best image appropriate to the needs
Uniform coloration
Excellent images with uniform coloration are now possible, at any magnifications, by changing the material composition of the DIC prisms.
The perfect balance of high contrast and high resolution
The standard DIC prisms for the system condenser can cover observation at 10X-100X with only two modules that are greatly balanced in contrast and resolution at any magnification. To further closely fit your specific observation needs and specimens, the high contrast DIC prisms and high resolution DIC prisms are also selectable.
Observe ultra-minute structures at full optical performance
High NA condensers (Dry, Oil) are available to specifically address the needs for further detailed
DIC images of high magnification observations.
These configurations are optimized for a high resolution video enhanced contrast DIC system.
Ultra-high resolution DIC prisms consisting of a high transmission polarizer and analyzer for each dry and oil type condenser are selectable in addition to standard DIC prisms.
By combining epi-fluorescence and DIC it is easy to accurately locate fluorescent tagged structures or artifacts within a specimen.
Using Rotating Senarmont Compensation method
Rotate polarizer to change DIC contrast.
Polarizer de Senarmont
Compensator
1/4 Plate
DIC Prism
Slider
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12.
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Viewed with the conventional phase contrast method
Enables high-contrast imagery of minute living cells
Phase contrast is the most popular observation method for inverted microscopes. This method does not require the staining of the specimen, therefore you can observe and research precise structures of living cells without influencing the live organism.
New phase contrast— “Apodized” phase contrast
In addition to the conventional phase contrast objectives, Nikon developed the new Apodized phase contrast objectives to effectively reduce halos, which was considered troublesome with the conventional phase contrast method. The internal structures of cells ongoing cell division or thick phase objects used to submerge in halos, making observation difficult, but they are now visible with excellent contrast and a much wider tonal range.
For research level work, we are proud to introduce
CFI Plan Fluor ELWD ADL objectives, which excel in image flatness in addition to higher resolution and brightness.
Viewed with the Apodized method
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ADL objective series
3D-like images made easy
This technique permits observation of living specimens using plastic petri dishes, which is not possible with DIC. The combination of dedicated
HMC objectives and HMC condenser components creates high contrast 3D-like images of living transparent specimens without the halos seen under phase contrast.
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Note: Hoffman Modulation Contrast and HMC are registered trademarks of Modulation Optics, Inc.
• 32-channel simultaneous acquisition suppresses damage to specimens.
• Selectable wavelength resolution from 2.5, 5 or
10nm, independent of pin-hole diameter.
• Acquisition of accurate fluorescence spectra enables color rendering of fluorescence images with greater realism.
• Polarization-enhanced optical sensitivity with DEES improves brightness.
• DISP (Dual Integration Signal Processing) eliminates digitization dead time.
• Spectral imaging via simple switchover from a
3-channel PMT detector.
Overlay of 32-channel images acquired with one shot
Time-lapse recording of 320nm wide spectra
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Fluorescence power
(pixel value)
3500
Time
(sec.)
1680
1440
1200
960
720
480
240
3000
2500
2000
1500
1000
500
0
530 545 560 575 590 605
Fluorescence wavelength (nm)
0
• Simultaneous 3-channel fluorescence, 3-channel plus DIC, time-lapse recording and spatial analysis are possible.
• Filters can be easily exchanged by users to match the latest fluorescent dyes.
• ROI scanning is possible with an optional AOM (Acousto Optical
Modulator)—perfect for FRAP (Fluorescence Recovery After Photobleaching).
• Bi-Directional Scan improves frame rates. Scan Rotation is also possible.
• A greater variety of lasers can be mounted.
Bleached
16.
After 2 sec.
After 30 sec.
After 120 sec.
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• A unification of a laser TIRF unit and epi-fluorescence illumination system.
Switching the systems is elementary.
• Responds to various levels of research such as from epi-fluorescence observation of living organisms to observation of living cells at the molecular level.
• Captures single molecule activity in living cells with an extraordinary high
S/N ratio where they contact the coverglass.
• The TE2000’s unique “stratum structure” allows the simultaneous mounting of laser tweezers.
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Image under TIRF observation Image under epi-fluorescence observation
TIRF/epi-fluorescence image overlay (pseudo-color)
• A TIRF function has been provided with the epi-fluorescence attachment. TIRF observation using mercury illumination is available. Xenon and high-intensity halogen can also be used.
• Simply inserting an exclusive aperture (60X, 100X) enables switching to TIRF.
• The wide wavelength band of mercury illumination makes multiple wavelength observation possible by changing the filter. No worries for interference patterns.
Mercury TIRF image Epi-fluorescence image
• Compact and easy to attach, easy to operate.
• Observation of molecule movement by photic stimulation in a cell is possible, using fluorescence protein such as Kaede (photo conversion) and PA-GFP
(photo activation).
• Achieves short wavelength correction up to 405nm (h-line). Combined with VC series objectives, in which aberration is corrected to 405nm, it illuminates the targeted area with high precision.
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Time-lapse images after stimulation can be recorded in series.
A packaged set of instrumentation required for cellular micromanipulation, the NT-88-V3 is ideal for IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection), electrophysiology, or biotechnology applications.
This all-in-one compact CO
2 incubator sustains the internal temperature at 37ºC with humidity of 90% and CO
2 of 5% to keep the specimen in a stable and precise condition. A special technique that minimizes focus blur facilitates long hours of time-lapse imaging.
With an acrylic plastic enclosure providing easy access to the specimen area, this accessory utilizes warm air circulation and maintains the temperature of the interior at 37ºC. The temperature is also adjustable from room temperature to
40ºC.
A temperature controllable stage ring with a glass heating plate keeps the specimen at a set temperature. Temperature is adjustable from room temperature to 50ºC in 0.1ºC increments.
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The high-definition cooled color digital camera with a Peltier cooling mechanism captures weak fluorescing images clearly by minimizing background noise.
• Super high resolution images with 12.6-mega output pixels.
• High sensitivity reduces shooting time and avoids photobleaching.
• High 15-fps (maximum) transfer rate ensures smooth live images.
• Easy-to-use control software facilitates large-volume shooting.
The Digital Sight series offers a choice of five camera heads and two control units, enabling an image capturing system to be assembled to suit each use.
High-speed color camera head DS-2Mv
• High frame rate, 2.0-megapixel color CCD.
• Sharp display of live images. Realizes high quality images.
High-speed monochrome camera head DS-2MBW
• High-frame-rate and high-sensitivity 2.0megapixel monochrome CCD.
• Sharp display of live images. Reduces photobleaching due to shorter shooting time.
Configured with DS-2MBWc-U2
High-speed cooled monochrome camera head DS-2MBWc
• Cooling mechanism retains CCD at room temperature minus 20°C.
• Reduces heat noise. Captures fluorescence and darkfield images clearly.
• High-frame-rate and high-sensitivity 2.0megapixel monochrome CCD.
• Sharp display of live images. Reduces photobleaching due to shorter shooting time.
High-definition/high-speed color camera head DS-Fi1
• High-definition 5.0-megapixel color CCD.
• High resolution and high frame rate.
• High dynamic range and accurate color reproduction.
• Reduces noise.
High-definition cooled color camera head DS-5Mc
• Cooling mechanism retains CCD at room temperature minus 20°C.
• Reduces heat noise. Captures fluorescent and darkfield images clearly.
• High-definition 5.0-megapixel color CCD.
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PC-use control unit DS-U2
• Compact, space-saving design.
• High-speed image transfer to PC via USB
2.0 connection.
• Versatile image capture, processing, measurement and analysis when coupled with imaging software NIS-Elements.
• Allows control of Nikon motorized microscopes.
Standalone control unit DS-L2
• Large 8.4-in. LCD monitor (XGA).
• Pre-programmed imaging modes for different observation methods.
• Various digital interfaces including USB 2.0
connection.
• Direct print possible.
• Allows control of Nikon motorized microscopes.
Nikon’s NIS-Elements software provides an integrated solution by delivering automated intelligence to microscopes, cameras, components and peripherals.
It handles multi-dimensional imaging tasks with support for capture, display, peripheral device control, and data management & analysis of images.
The database building feature for handling large numbers of multi-dimensional image files enables efficient and fully documented experiments. The intuitive interface simplifies workflow, and fast image acquisition speeds via direct streaming to RAM allow the recording of rapid biological events. The software also supports numerous image processing capabilities including binary and morphological tools for measurement and analysis routines.
The unified control of the entire imaging system offers significant benefits for cutting-edge research applications such as live cell imaging.
Three distinct packages for specific application requirements are available.
Ar
NIS-Elements AR (Advanced Research)—for fully automated acquisition and device control through full 6D
(X, Y, Z, Lambda (wavelength), Time, multipoint) image acquisition and analysis.
Br
D
NIS-Elements BR (Basic Research)—for acquisition and device control through 4D (such as X, Y, Z, Time and X, Y,
Z, Lambda (wavelength)) acquisition.
NIS-Elements D (Documentation)—for supporting color documentation requirements in bioresearch, clinical and industrial applications, with basic measuring and reporting capabilities.
Real time 2D deconvolution
Allows one-click image acquisition and transfer from a camera to a user defined database.
The database supports image and meta-data export to a report generator, enabling users to create report templates and printed or PDF-based reports.
Ultra-high resolution images can be captured with a motorized stage using sophisticated auto focus.
Allows the creation of an all-in-focus image from a series of
Z-axis image stacks providing 3D modeling capability and a multi-dimensional image viewer.
Supports live on-the-fly or captured deconvolution of an entire image, or specific regions of interest.
Visit www.nis-elements.com for more detailed information.
Merge channels
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Description
Brightfield
Achromat flat field
Plan Achromat
Fluor
Plan Fluor
CFI Achromat 4X
CFI Achromat 10X
CFI75 LWD 16XW
CFI Achromat LWD 20X
CFI Achromat 40X
CFI Achromat LWD 40XC
CFI Achromat 60X
CFI Achromat 100X oil
CFI Achromat 100X oil, iris
CFI Plan Achromat UW 1X
CFI Plan Achromat UW 2X
CFI Plan Achromat 4X
CFI Plan Achromat 10X
CFI Plan Achromat 20X
CFI Plan Achromat 40X
CFI Plan Achromat 40X NCG
CFI Plan Achromat 50X oil
CFI Plan Achromat 100X oil
CFI Plan Achromat 100X WI
CFI Plan Achromat 100X NCG
CFI Fluor 10X W
CFI Fluor 20X W
CFI Fluor 40X W
CFI Fluor 60X W
CFI Plan Fluor 4X
CFI Plan Fluor 10X
CFI Plan Fluor 10XW
CFI Plan Fluor 20X
CFI Plan Fluor ELWD 20XC
CFI Plan Fluor 20X MI
Apochromat
Plan Apochromat
CFI Plan Fluor 40X
CFI Plan Fluor 40X oil
CFI Plan Fluor ELWD 40XC
CFI Plan Fluor 60XC
CFI Plan Fluor 60X oil, iris
CFI Plan Fluor ELWD 60XC
CFI Plan Fluor 100X dry
CFI Plan Fluor 100X oil
CFI Plan Fluor 100X oil, iris
CFI Apo 40XW NIR
CFI Apo 60XW NIR
CFI Apo TIRF 60X oil
CFI Apo TIRF 100X oil
CFI Plan Apochromat 2X
CFI Plan Apochromat 4X
CFI Plan Apochromat 10X
Plan Apochromat VC
CFI Plan Apochromat 20X
CFI Plan Apochromat 40XC
CFI Plan Apochromat 40X oil
CFI Plan Apochromat 60XC
CFI Plan Apochromat 100X Oil
CFI Plan Apochromat 100X NCG oil
CFI Plan Apochromat VC 60X oil
CFI Plan Apochromat VC 60X WI
CFI Plan Apochromat VC 100X oil
Plan Apochromat TIRF
CFI Plan Apochromat TIRF 60X oil
S Fluor
CFI Plan Apochromat TIRF 100X oil
CFI S Fluor 4X
CFI S Fluor 10X
CFI S Fluor 20X
CFI S Fluor 40XC
CFI S Fluor 40X oil
CFI S Fluor 100X oil, iris
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NA W.D. (mm)
0.90
1.25
1.10
0.90
0.30
0.50
0.80
1.00
0.13
0.30
0.3
0.50
0.45
0.10
0.25
0.8 (at 16X)
0.40
0.65
0.55
0.80
1.25
0.5-1.25
0.04
0.06
0.10
0.25
0.40
0.65
0.65
0.75
0.13
0.13
0.13
15.5
1.2
1.0
0.3
0.22
0.2
1.0
0.14
0.16
0.15
0.13
0.17
0.13
0.27
0.2
3.5
2.8
0.13
0.12
8.5
15.7
4.0
0.72
0.2
3.7-2.7
0.3
0.22
2.1-1.5
0.3
0.2
2.0
2.0
2.0
2.0
0.4
0.17
2.5
0.26
17.1
16.0
3.5
2.1
8.1-7.0
Oil 0.35;
Glycerin 0.34;
Water 0.33
0.23
3.2
7.5
30.0
10.5
1.3
0.57
0.48
30.0
7.0
3.0
3.8
0.65
2.7-1.7
0.3
0.23
0.75
0.95
1.00
0.95
1.40
1.40
1.40
1.20
1.40
1.45
1.45
0.20
0.50
0.75
0.90
1.30
0.5-1.30
0.5-1.3
0.8
1.00
1.49
1.49
0.10
0.20
0.45
0.75
1.30
0.60
0.85
0.5-1.25
0.70
0.90
1.30
Remarks
Water dipping
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Water dipping
Water dipping
Water dipping
Water dipping
Water dipping
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
C.C.0.5-1.5
Spring loaded
Spring loaded
Spring loaded
Water dipping
Water dipping
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
C.C.0-2 with iris
No cover glass with temperature correction ring
No cover glass
C.C.0-2
Multi-immersion; Oil-glycerin-water
Stopper
C.C.0-2
C.C.0.11-0.23
with iris
C.C.0.14-0.2
Stopper with iris
C.C. 0.13-0.22
C.C. 0.13-0.20
C.C.0.11-0.23
Stopper
C.C.0.11-0.23
Stopper
CC.0.15-0.18; Water-immersion
Stopper
C.C. 0.10-0.22
CG 0.17
C.C.0.11-0.23
Description
Phase Contrast
Achromat flat field
Plan Achromat
Fluor
Plan Fluor
Plan Apochromat
S Fluor
Hoffman Modulation Contrast
®
CFI HMC 10X
CFI HMC LWD 20XF
CFI HMC LWD 40XC
Polarizing
CFI Achromat DL 10X
CFI Achromat ADL 10X
CFI Achromat LWD DL 20X
CFI Achromat LWD DL 20XF
CFI Achromat LWD ADL 20XF
CFI Achromat DL 40X
CFI Achromat LWD DL 40XC
CFI Achromat LWD ADL 40XF
CFI Achromat LWD ADL 40XC
CFI Achromat DL 100X oil
CFI Achromat BM 10x
CFI Plan Achromat DL 10X
CFI Plan Achromat DL 20X
CFI Plan Achromat DL 40X
CFI Plan Achromat DL 100X oil
CFI Fluor DLL 40XW
CFI Plan Fluor DL 4X
CFI Plan Fluor DLL 10X
CFI Plan Fluor DL 10X
CFI Plan Fluor DLL 20X
CFI Plan Fluor ELWD DM 20XC
CFI Plan Fluor ELWD ADL 20XC
CFI Plan Fluor DLL 40X
CFI Plan Fluor ELWD DM 40XC
CFI Plan Fluor ELWD ADL 40XC
CFI Plan Fluor ELWD DLL 60XC
CFI Plan Fluor DLL 100X oil
CFI Plan Fluor ADH 100X oil
CFI Plan Apochromat DM20X
CFI Plan Apochromat DM40XC
CFI Plan Apochromat DM40X oil
CFI Plan Apochromat DM60XC
CFI Plan Apochromat DM60X oil
CFI Plan Apochromat DM100X oil
CFI S Fluor DL 20X
CFI S Fluor DL 40X
CFI Achromat P 4X
CFI Achromat P 10X
CFI Achromat LWD P 20X
CFI Achromat P 40X
CFI Achromat P 100X Oil
NA
0.25
0.40
0.55
0.10
0.25
0.40
0.65
1.25
0.45
0.45
0.75
0.60
0.60
0.70
1.30
0.65
1.25
0.80
0.13
0.30
0.30
0.50
1.30
0.75
0.95
1.0
0.95
1.40
1.40
0.75
0.90
0.25
0.25
0.40
0.40
0.40
0.65
0.55
0.55
0.55
1.25
0.25
0.25
0.40
W.D. (mm) Remarks
6.1
3.0
2.7-1.7
30.00
7.00
3.80
0.65
0.23
0.57
0.17
2.0
16.4
16.0
15.2
2.1
8.1-7.0
8.1-7.0
0.72
3.7-2.7
3.7-2.7
2.1-1.5
0.2
0.2
1.0
0.14
0.16
0.15
0.21
0.13
1.00
0.30
7.0
6.2
3.8
3.0
3.0
0.65
2.7-1.7
2.1
2.7-1.7
0.23
6.10
10.5
1.3
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Water dipping
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
Spring loaded
CG : Cover Glass thickness (mm)
CC : Correction Collar (mm)
Type
T-CT-E Motorized System
Condenser/System Condenser
High NA Condenser
TE-C ELWD Condenser
TE-C SLWD Condenser
TE-C HMC Condenser
ELWD condenser lens
LWD condenser lens
HMC condenser lens
Dry top lens
Water immersion top lens
Oil immersion top lens
NA
0.3
0.52
0.4
0.85
0.9
1.4
0.3
0.12
0.4
W.D. (mm) Ph module
75 L.1.2
30
44
L.1.2.3
5
4
1.92
75
190
44
L.1.2.3
L.1
HMC module DIC module
MC1.MC2.MC3
LWD N1, LWD N2, LWD NR
MC1.MC2.MC3
HNA N2, HNA NR
HNA N2, HNA NR
Magnifications
2-60X
4-100X
10-40X
10-100X
10-100X
10-100X
2-20X
4-40X
10-40X
CG 1.2
CG 1.2
CG 1.2
C.C.0-2
CG 1.2
C.C.0-2
CG 1.2
CG 1.2
C.C.0-2
C.C.0-2
C.C.0-2
C.C.0-2
C.C.0.5-1.5
Stopper
Stopper
C.C.0.11-0.23
Stopper
C.C.0.11-0.23
Stopper
Stopper
C.C.0.11-0.23
Ph1
Ph1
Ph2
Ph2
Ph2
Ph2
Ph3
Ph2
Ph3
Ph2
PhL
Ph1
Ph1
Ph1
Ph3
Ph2
Ph2
Ph3
Ph2
Ph3
Ph3
Ph2
Ph2
Phase ring
Ph1
Ph1
Ph1
Ph1
Ph1
Ph2
Ph2
Ph1
Ph2
Ph3
Ph1
Ph1
Ph1
CG 1.2
CG 1.2
C.C.0-2
23
U
V
V
B
V
B
G
Y
Filters
UV-1A
UV-2A
UV-2B
UV-2E/C
(DAPI)
V-2A
BV-1A
BV-2A
B-1A
B-1E
B-2A
B-2E/C
(FITC)
B-3A
G-1B
G-2A
G-2B
G-2E/C
(TRITC)
Y-2E/C
(Texas Red)
Wavelengths
EX 365/10
DM 400
BA 400
EX 330-380
DM 400
BA 420
EX 330-380
DM 400
BA 435
EX 340-380
DM 400
BA 435-485
EX 380-420
DM 430
BA 450
EX 435/10
EM 455
BA 470
EX 400-440
DM 455
BA 470
EX 470-490
DM 505
BA 520
EX 470-490
DM 505
BA 520-560
EX 450-490
DM 505
BA 520
EX 465-495
DM 505
BA 515-555
EX 420-490
DM 505
BA 520
EX 546/10
DM 575
BA 590
EX 510-560
DM 575
BA 590
EX 510-560
DM 575
BA 610
EX 540/25
DM 565
BA 605/55
EX 540-580
DM 595
BA 600-660
Characteristics
•Narrow band pass – only 365nm (i line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
•Standard filter block for UV
•Darker background than UV-2A
•For DAPI, cutting off FITC (green) and TRITC (red)
•Soft-coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off green and red
•Standard filter block for V
•Narrow band pass – only 435nm (g line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
•Standard filter block for BV
•Narrower excitation range than B-2A
•FITC + Counter-stain (TRITC, PI)
•For FITC (green), cutting off Rhodamine red
•Band-Pass Barrier Filter used to cut off red
•Standard filter block for B
•For FITC + Counter-stain (TRITC, PI)
•Soft coated type for high signal/noise
•For FITC (green), cutting off Rhodamine red
•Band-pass Barrier Filter used to cut off red
•Wide band pass – recommended for halogen illumination only
•Narrow band pass – only 546nm (e line) of Mercury spectrum used
•Narrow band pass minimizes auto-fluorescence and photo-bleaching
•Standard filter block for G
•610nm barrier provides darker background and deep red emission
•For TRITC (Rhodamine)
•Soft coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off reds above 643nm
•For Texas Red ®
•Soft coated type for high signal/noise
•Band-Pass Barrier Filter used to cut off reds above 660nm
Filters
Dual
Abbreviations
F-R
F-T
D-F
Applications
FITC
Rhodamine
FITC
Texas Red
DAPI
FITC
Models
GFP-L
GFP-B
Wavelengths
EX480/40, DM505, BA510
EX480/40, DM505, BA535/50
Characteristics
GFP long-pass type
GFP band-pass type
Triple
Filters
Applications
GFP
GFP
Applications
•DAPI
•Hoechst 33258/33342
•AMCA
•Cascade Blue ®
•Autofluorescence
•Catecholamine
•Serotonin
•Tetracycline
•Quinacrine
•Quinacrine Mustard (QM)
•Thioflavine S
•Acriflavine
•FITC
•Acridine Orange
•Auramine O
•Coriphosphine O
•Bodipy ®
•Fluo-3
•DIO
•TRITC
•Rhodamine B200
•Propidium iodide
•R-Phycoerythrin
•B-Phycoerythrin
•Dil
•Ethidium Bromide
•Texas Red ®
Abbreviations
D-F-R
D-F-T
Applications
DAPI
FITC
Rhodamine
DAPI
FITC
Texas Red
Each filter/mirror has a very sharp rising edge at the corresponding wavelength, minimizing signal crossover.
Filters
CFP HQ
GFP HQ
YFP HQ
Wavelengths
EX420-445, DM450, BA460-510
EX455-485, DM495, BA500-545
EX490-500, DM510, BA520-560
24
Main body
Optical system
CFI60 infinity optical system, parfocal distance 60mm
Light distribution
5 positions, motorized light-distribution changer
Observation 100, Left port 100, Right port 80, Front port 80, Bottom port
100
* 3 other models are available as options:
1) left 80/100 switchable instead of bottom 100
2) right 80/100 switchable instead of bottom 100
3) front 80/100 switchable instead of bottom 100
4 positions
Observation 100, Left port 100,
Right port 80, Front port 80
* 4 other models are available as options for 5-position light distribution:
1) with bottom port (must be added,
100% light)
2) left 80/100 switchable
3) right 80/100 switchable
4) front 80/100 switchable
2 positions
Observation 100, Left port 80
* 2 other models are available as options:
1) left 100 instead of 80
2) right port (must be added) 100 instead of left 80
PFS
Compatible specimen
Compatible dish
Detect boundary
Specimens in aqueous solutions
(cultured specimens, in-vitro assays, etc.)
Glass-bottom dishes (thickness: 150-
180µm, No. 15 recommended)
Offset distance Adjustable with the controller dial
Memory function
Offset distance, store/recall of nosepiece up/down distance (up to 6 points per nosepiece)
Focusing
Immersion lens: between glass and medium; between dry lens: glass and air
——
Via motorized/manual nosepiece up/down movement
Stroke—manual: up 7mm, down 3mm; motorized: up 6mm, down 2.5mm
Coarse stroke: 4.9mm/rotation; Fine stroke: 0.1mm/rotation (motorized)
Minimum fine reading: 0.05µm by optical linear encoder
External fine focusing unit
Via nosepiece up/down movement
Stroke—manual: up 7mm, down
3mm
Coarse stroke: 4.9mm/rotation; Fine stroke: 0.1mm/rotation
Minimum fine reading: 1µm
Refocusing stopper: Adjustable coarse torque stopper
Intermediate magnification
Extendible structure
Other
Eyepiece tube
1.5X
Available
Light intensity control; Light on/off switch
(1) T-TD Binocular Tube D
(2) T-TS Binocular Tube S
(3) T-TERG Binocular Ergonomic Tube D
(4) T-TI Intermediate Tube for Eclipse E600/400 trinocular tubes and teaching heads
Eyepiece lens
(F.O.V.)
Illumination
Condenser
CFI 10X (22mm), CFI 12.5X (16mm), CFI 15X (14.5mm)
Nosepiece
Objectives
Stage
Via nosepiece up/down movement
Stroke—manual: up 7mm, down
3mm
Coarse stroke: 4.9mm/rotation; Fine stroke: 0.1mm/rotation
Minimum fine reading: 1µm
Adjustable coarse torque stopper
——
(1) T-DH 100W Illumination Pillar (2) T-DS 30W Illumination Pillar
SLWD condenser for phase contrast*, ELWD condenser for phase contrast*, System condenser LWD**, ELWD** Motorized system condenser
LWD**, ELWD**, Hoffman Modulation condenser® (HMC), High NA condensers
*Only for 30W pillar **Only for 100W pillar
T-N6 Sextuple Nosepiece, T-ND6 Sextuple DIC Nosepiece, T-ND6-E Motorized Sextuple DIC Nosepiece
CFI60 objectives
(PFS function does not work with some objectives.)
CFI60 objectives
(1) T-SR Rectangular Stage—Cross travel: 70 x 50mm; Size: 300 x 276mm
Motorized functions
Epi-fluorescence attachment
Nomarski DIC system
(2) T-SP Plain Stage—Size: 300 x 210mm; Mechanical stage mountable
(3) T-SAM Attachable Mechanical Stage (must be used with T-SP Plain Stage)—Cross travel: 126 x 84mm; Specimen holders attachable
Fine focusing (minimum reading: 0.05µm), Objective anti-collision mechanism (when the nosepiece is rotated), Light distribution changer
—— ——
(Motorized options):
DIC nosepiece, Analyzer, Epi-fl filter rotating turret, Epi-fl shutter, Excitation filter wheel, Barrier filter wheel, System condenser turret
Six fluorescence filter blocks in rotating turret with shutter, Noise Terminator mechanism incorporated, Aperture diaphragm centerable, Field diaphragm centerable, 33mm ND4/ND8 filters, 25mm heat absorbing filter, Lamphouse adapter, Zoom lamphouse adapter (option)
Contrast control: Senarmont method (by rotating polarizer)
Objective side prism: for individual objectives (installed in nosepiece)
Condenser side prism: LWD N1/N2, HNA N2/NR types
*Nomarski DIC system can be attached only to 100W pillar.
Optional accessories
Nikon Digital SLR D1, Digital Cameras DXM1200C/Digital Sight series/DQC-FS, Photomicrographic Equipment H-III, Teaching Head, Drawing Tube,
CCTV Adapters, Micromanipulators, etc.
Power consumption
(max.)
Weight (approx.)
(TE2-PS 100W Power Supply) 100-230V, 2.4A/160W (TE-PS 30W Power Supply) 100/120V, 0.6A/80W; 230V, 0.4A/80W
Phase contrast set: 45kg; Epi-fl set: Phase contrast set: 40kg; Epi-fl set:
50kg 45kg (w/100W pillar)
Phase contrast set: 36kg; Epi-fl set:
41kg (w/100W pillar)
Phase contrast set: 32kg
(w/30W pillar)
25
HMC MC1, MC2,
MC3 Modules
HMC Lens
Eyepieces
CFI 10X,
12.5X, 15X
Centering
Telescope
Y-TT2 Trinocular Tube T2UW Y-TF2 Trinocular Tube F2UW
Y-IDT Drawing Tube
Teaching Heads
1
Eyepiece
Tubes
Phase PH-L, PH-1,
PH-2 Modules
ELWD Lens
System Condenser
Turret
Phase PH-L, PH-1,
PH-2, PH-3;
DIC LWD N1,
LWD N2;
HMC MC1,
MC2, MC3 Modules
LWD Lens
T-CT-E Motorized
System Condenser
Turret*
2
T-TD
Binocular Tube D
T-TS
Binocular Tube S
T-TERG
Binocular
Ergonomic Tube D
T-TI
Intermediate
Tube
Eye-level
Riser
5
Condensers
35mm Petri
Dish Holder
Stage Ring
C-HSG Slide
Glass Holder
Glass
Stage Ring
Hemacytometer
Holder
C-HU Universal
Holder
T-SAM
Attachable Mechanical
Stage
Acrylic
Stage Ring
2
Stages
T-SSR
Short
Rectangular
Stage
T-SR
Rectangular
Stage
T-SP Plain Stage
C-Mount
Adapter A C-Mount TV
Adapter VM4X
C-Mount TV
Adapter VM2.5X
C-Mount
CCTV
Camera
C-Mount
Adapter
0.45X, 0.6X
C-Mount
CCTV
Adapter
Relay
Lens 1X
C-0.7X
DXM
Relay Lens
C-Mount
Zoom
Adapter
TV
Zooming
Lens
ENG-Mount
CCTV
Camera
Photomicrographic
System FX-III Series
Adapters
T-BPA
Photo
Adapter
DIC HNA-N2,
Modules
DIC HNA-N2,
HNA-NR
Modules
High NA Lens
(Dry)
High NA Lens
(Oil)
High NA
Condenser
Holder
High NA
Condenser
Lens Unit
CFI Objectives
TE-C ELWD
Condenser
DIC Slider
T-N6 Sextuple
Nosepiece
T-ND6 Sextuple
DIC Nosepiece
T-ND6-E Motorized
Sextuple DIC Nosepiece* 2
6
Nosepieces
T-FLBW-E Motorized
Barrier Filter Wheel*
2
4 Side Port
Multi-image
Module
35mm SLR
Camera
T-BSLR
Front-port
SLR Adapter
High-definition
Digital Camera
DXM1200
C-mount TV
Camera T-BDCA
Front-port
Direct C
Adapter
F-mount
Digital
Camera D1
T-BFA Front-port
F-mount Adapter
3 Front Port
T-PFS
Perfect
Focus Unit
T-FLC-E
Motorized
Epi-fl Filter
Rotating Turret
Eyepiece
Tubes
1
Stages Nosepieces
2
9
Stage Riser
* 3
6
Epi-fl
Attachments for PFS
8’
REROCUS
COARSE FOCUS
ESCAPE
Bottom Port
* 3 Essential for PFS.
7
Illumination
Pillars
10
Analyzer
4 Side Port
T-HUBC
Communications
Hub Controller
T-PFS Perfect Focus
Controller
DO
W
N
T-EFN Focus Knob
T-RCP
Remote
Control Pad
T-AC
AC Adapter
26
Lambda Plate
T-P Polarizer
Halogen Lamp
12V-100W
Filter 45mm;
GIF, NCB11, ND16, ND2
Heat Absorbing
HMX
Lamphouse
Adapter
Collector
Lens
Halogen Socket
100W
SLWD Condenser
Filter 33mm;
GIF, Heat Absorbing,
NCB11, ND16, ND2
HMX
Lamphouse
Halogen Lamp
6V-30W
Epi-fl
Attachments
8
5
Condensers
T-DH
100W Illumination Pillar
T-FLC Epi-fl Filter Rotating Turret
Epi-fl
Filter
Blocks
T-FL
Epi-fl
Attachment
Halogen Lamp
12V-100W LL
Adapter
* 1
Lamphouse-2 BL
Remote Cable
TE2-PS
12V Power Supply for
100W Illumination Pillars
HMC MC1, MC2,
MC3 Modules
System Condenser
Turret
HMC Lens
TE-C ELWD
Condenser
7
Illumination Pillars
T-FLA
FL Illumination
Adapter
T-FLEW-E
Motorized Excitation
Filter Wheel* 2
TE-AT
Double
Lamphouse
Adapter
C-FC
Epi-fl
Collector
Lens
T-DS
30W Illumination Pillar
Remote Cable
Lamphouse
HMX-3B/HMX4B w/Backmirror
Mercury Lamp
Socket S 100W
TE-PS
6V Power Supply for 30W Illumination Pillar
* 1 Provided with the HMC lens.
Mercury
Lamp
HG-100W
Starter
HG-100W
T-FLZA
FL Zoom
Illumination
Adapter
Epi-fl
Collector
Lens Q2
Xenon
Starter
Xenon
Lamp
T-FLC-E Motorized Epi-fl Filter
Rotating Turret*
2
Epi-fl Attachments for PFS
8’
T-FL-E Epi-fl
Attachment with
Motorized
Shutter* 2
Xenon
Lamphouse
Xenon
Lamp Socket
Halogen
Lamp
12V-100W
Stage Riser
9
T-BSUK70
Stage Riser
T-BSUK70
Stage Riser
Analyzer
10
T-A Analyzer
T-A-E Motorized
Analyzer* 2
HMX
Lamphouse
U Transformer
Halogen Socket
100W
Front Port
3
Eyepiece
Tubes
1
Stages
2
Nosepieces
Illumination
Pillars
7
9
Stage Riser
6
Epi-fl
Attachments
8
10
Analyzer
T-HUBC
Communications
Hub Controller
Front Port
3
Eyepiece
Tubes
1
Stages
2
Nosepieces
Illumination
Pillars
6 7
9
Stage Riser
Epi-fl
Attachments
8
Analyzer
10
T-HUBC
Communications
Hub Controller
Eyepiece
Tubes
1
Stages
2
Nosepieces
Illumination
Pillars
7
9
Stage Riser
6
Epi-fl
Attachments
8
Analyzer
10
T-HUBC
Communications
Hub Controller
REROCUS
COARSE FOCUS
ESCAPE
Bottom Port 4 Side Port
T-AC
AC Adapter
DO
W
N
T-EFN Focus Knob
T-RCP
Remote
Control Pad
Model with bottom port also available
4
Side Port
T-AC
AC Adapter
T-RCP
Remote
Control Pad
4
Side Port
(left side)
T-AC
AC Adapter
T-RCP
Remote
Control Pad
*
2
Requires a communications hub controller.
27
Unit: mm (inch)
144.5 (5.7)
Eyepoint
163.5 (6.4)
195 (7.7)
260 (10.2)
144.5 (5.7)
19.6 (0.8) 476.4 (18.8)
618 (24.3)
Eyepoint
163.5 (6.4)
144.5 (5.7)
Eyepoint
163.5 (6.4)
195 (7.7)
260 (10.2)
144.5 (5.7)
41.3 (1.6)
COARSE FOCUS
ESCAPE
476.4 (18.8)
598 (23.5)
Eyepoint
154.6 (6.1)
195 (7.7)
260 (10.2)
41.3 (1.6) 135.4 (5.3)
476.4 (18.8)
Photos courtesy of:
3
4
1 JC1 stained (Mitochondrial membrane potential) Murine 2 cell embryo, In-vivo derived—
David Froiland Bsc., Deidre Zander Bsc. Hons., Michelle Lane PhD., Research Centre for
Reproductive Health, University of Adelaide, Australia
2 Albino Swiss mouse embryo fibroblast cell
6, 7, 9, 10, 16, 17
Michael W. Davidson, National High Magnetic Field Laboratory, USA
5
Aplysia bag cell neuronal growth cone—Dylan Burnette, Paul Forscher Laboratory, Yale University, USA
Profs. Akihiro Kusumi and Dr. Chieko Nakada, Kusumi Laboratory, Institute for Frontier Medical
Sciences, Kyoto University
Dr. Chieko Nakada, Kusumi Office, Institute for Frontier Medical Sciences, Kyoto University
Co-researcher: Professor Shigeo Okabe, Tokyo Medical and Dental University.
195 (7.7)
260 (10.2)
135.4 (5.3)
476.4 (18.8)
13
14
15
8
11
12
16
17
Neut lung cells in culture—Alexey Khodjakov, PhD., Wadsworth Center, NY Dept. of Health, USA
Sea urchin egg—Yukihisa Hamaguchi, PhD, Tokyo Institute of Technology, Japan
Rat embryo in cell division (EGFP transgenic rat)—Masumi Hirabayashi, PhD, YS New Technology
Inst. Inc., Japan
Monkey kidney cells—Satoko Takaoka, Utsunomiya East Hospital, Japan
Bovine ovum—YS New Technology Inst. Inc., Japan
JC-1 loaded nerve cells—Dr. Yasushi Okada, Cell Biology, Medical Dept of Graduate School, Tokyo
University
Hela cell nucleus expressing H1 Histon-GFP—Dr. Hiroshi Kimura, Horizontal Medical Research
Organization, Kyoto University Faculty of Medicine
Fixed 3T3 fibroblasts—Dr. Gregg G. Gundersen, Columbia University, USA
Please contact Nikon for a handy pamphlet listing compatible accessories, including objectives and epi-fluorescence filters.
Some models are not available in certain areas. Please check with your local Nikon representative for details.
Enter the “Microscopy University” on the web and discover a whole new world.
www. microscopyu.com
Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. March 2006. ©2006 NIKON CORPORATION
WARNING
TO ENSURE CORRECT USAGE, READ THE CORRESPONDING
MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.
* Monitor images are simulated.
CompactFlash is a trademark of SanDisk Corporation, Sunnyvale, CA, U.S.A. Company names and product names appearing in this brochure are their registered trademarks or trademarks.
NIKON CORPORATION
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UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584
NIKON INSTRUMENTS INC.
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phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306
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Nikon promotes the use of eco-glass that is free of toxic materials such as lead and arsenic.
Printed in Japan (0603-09.2)T
Code No. 2CE-MTWH-8
This brochure is printed on recycled paper made from 40% used material.
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Perfect Focus System
Noise Terminator
Motorized focus
5-way light port changeover
Extendible configuration
CFI60 optical system
Multiport design
Ergonomic design
Retrofittable motorized options
Epi-fluorescence
The Perfect Focus System (PFS) automatically detects the surface of the coverslip and continually corrects focus to compensate for drift. This ensures accurate focus for long-term observations and stage movement.
The Noise Terminator mechanism minimizes noise and increases fluorescence image contrast by absorbing stray light in the optical path.
The TE2000 supports a variety of observation methods, including epi-fluorescence, Nomarski DIC, phase contrast, and Hoffman Modulation Contrast.
The TE2000's extendible configuration allows for the introduction of equipment such as laser tweezers or extra illumination into the optical path without modifying the microscope body.
The TE2000 accepts retrofittable motorized accessories, including a motorized condenser turret, a motorized sextuple DIC nosepiece, a motorized epi-fl filter rotating turret, a motorized barrier filter wheel, and more.
What are the dimensions of the microscope?
The height of the microscope ranges from 712.8 mm (28.1 inches) to 783 mm (30.8 inches), and the width is 260 mm (10.2 inches). Other dimensions are available in the dimensional diagram in the manual.
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