Nikon ECLIPSE Ts2R Inverted Research Microscope Manual
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Inverted Research Microscope ECLIPSE Ts2R Inver ted Research Microscope A compact inverted microscope for your basic research needs Compact body Easy to work with Newly developed mechanical stage option Simple operations Control buttons on the Ts2R microscope are intuitively located for a streamlined workflow. The on/off and diascopic/epi-fluorescence switching controls are located on the front panel while buttons pertaining to either diascopic or DIA illumination epi-fluorescence brightness control are zoned adjuster to the left and FL illumination brightness adjuster right sides of the microscope body, Illumination respectively. LED ON/OFF EPI illumination DIA illumination switch switch switch Compact body for streamlined workflow Easily fits inside laminar flow hoods Illumination modules including the epi-fluorescence light source have been seamlessly incorporated into the microscope main body, resulting in a compact and simple design form that's also durable. The compact structure is also vibration-resistant to provide highly stable sample observations. The Ts2R can be configured with an optional rectangular mechanical stage. This stage provides a long travel stroke, enabling users to observe an entire well plate from end-to-end. You can also set limits to the travel stroke (three-way) to match frequently used samples or vessels. The stage handle is offered in two lengths, long or short, to further accommodate the user's needs. Epi-fluorescence LED Light Source The compact size of the Ts2R microscope makes it easier to store and operate inside a laminar flow hood. Additionally, the low stage and side-port camera position reduce user fatigue from repetitive stage manipulation and provides clear visibility of the stage and sample even with the hood sash lowered. Camera Port A stage with the handle attached to the left side is also available. Fluorescence Filter Cube Turret New ergonomic stage design for improved workflow 1 The Ts2R’s stage height has been lowered by approximately 30% compared to the standard research microscope*, thereby ensuring a comfortable hand position during repetitive operation and sample exchange. Moreover by lowering the support columns and positioning the camera port on the side of the tube, sample visibility is improved. 2 3 4 High precision and quality 5 6 Stroke adjustment using the XY limit pin (three way) *Comparison with Nikon’s Inverted Research Microscope ECLIPSE Ti. 1 2 3 4 5 6 Slide Glass Holder Petri Dish Holder 35mm Petri Dish Holder 100mm Well Plate Clamper Terasaki Holder Universal Holder Advanced-optical performance The Ts2R is compatible with Nikon's acclaimed CFI60 objective lenses which provide high numerical apertures and long working distances to deliver stunningly clear images. High performance and quality optical accessories Versatile applications with new LED illuminators The high-intensity LED light source enables you to perform a wide variety of observation methods similar to the full-size, inverted research microscope ECLIPSE Ti. The Ts2R-FL model, which offers epi-fluorescence observation, provides four fluorescence channels and 7 different wavelengths to choose from. 2 DIA FL Brightfield DIC APC (Apodized Phase Contrast) Optical accessories achieve the same performance level as Nikon's inverted research microscope ECLIPSE Ti, providing exceptionally clear, sharp images. Emboss Contrast Phase contrast NAMC (Nikon Advanced Modulation Contrast) Epi-fluorescence Diascopic illumination model Diascopic and epi-fluorescence illumination model 3 Do more than before DIA Diascopic observations with enhanced quality High-intensity LED Eco-illumination Application Do more than before FL Fluorescence images with uniform bright illumination Application Accurately reproduce illumination power every time Nikon's LED Eco-illuminator is environmentally friendly with its low power consumption and yet provides extremely bright illumination, suitable for phase contrast and DIC imaging. The built-in fly-eye lens ensures uniform brightness across the entire field of view. Furthermore, LED excitation has no unwanted UV component, thereby eliminating UV-mediated cell damage and improving cell survival rates during long-term imaging. The illumination power previously defined by the user is replicated when the same wavelength is used again, thus eliminating the need for manual adjustment of LED Unit light intensity Filter Cube when switching between wavelengths. Phase contrast observation Phase contrast is an optical contrasting technique that typically utilizes a phase contrast objective lens and condenser annulus. The use of a high-intensity LED light source results in clear images even at high magnifications. High S/N epi-florescence observation in bright rooms 10x 40x Apodized Phase Contrast (APC) observation APC observation is a type of phase contrast microscopy which minimizes unwanted halos in thick specimens. For example, APC technique provides clearer details of thick samples such as dividing cells. New Contrasting Technique, "Emboss Contrast" Overlapping image with three colors with use of Imaging Software NIS-Elements Nikon's new contrasting technique is compatible with both plastic and glass culture dishes. Unlike phase contrast or NAMC, Emboss Contrast does not require special objective lenses and therefore has minimal effect on epifluorescence observation. Emboss Contrast allows thick samples such as embryos to be easily observed in pseudo-three-dimensional image with great clarity. Multicolor fluorescence observation Using four different LEDs, multicolor fluorescence observation can be easily and efficiently achieved. Image courtesy of Hideaki Watanabe, Ph.D. and Hisataka Hasegawa, Ph.D. Spindle Observation System (optional, Released in April 2016) Accurate observation of spindle bodies is easily attained with the Ts2R. The system offers finely detailed work without damaging the spindle body. Nikon Advanced Modulation Contrast (NAMC) NAMC provides high relief, DIC-like images of samples on plated on plastic dishes, which is not possible with DIC observation. Ts2R provides high-quality NAMC images like Nikon's inverted research microscope, ECLIPSE Ti. 4 The new Contrast Shield accessory (optional) blocks room light, providing an easy and cost-effective method for achieving high signalto-noise fluorescence observation in a brightly lit laboratory. OPEN OPEN CLOSE CLOSE Differential Interference Contrast (DIC) observation DIC provides high-resolution pseudo-three dimensional images that have a shadowcast appearance. New high-intensity LED illumination results in vivid DIC images even at high magnifications. Fiber illumination High signal-to-noise fluorescence imaging Clear, vivid observation across the entire field of view Noise Terminator helps to capture vivid images. The fly-eye lens delivers uniform brightness to the entire field of view. As an alternative to the built in LED system, an external light source can be utilized through the liquid light guide port to accommodate users who require custom lighting needs. 5 Accessories System Diagram Thermo Plate TPX �������������� Thermo plate TPX provides accurate and stable temperature control for the specimen from room temperature to 50 degrees Celsius. Proprietary treatment methods ensure that the glass surface of the Termo Plate is breakage-free. ������� ������������ �������������� �������� �������� ����� ������ ������ ������ ���� ���� ������� ������� ������� ���� ������� ������ ���� ���� ������� ������� ���������� ������ ���������� ������ ������� ������ Manufacturer: TOKAI HIT Co., Ltd. Stage-top incubator A stage-top incubation chamber can be utilized to accurately control temperature, humidity and CO2 levels to maintain optimal cell health during long-term observation. Manufacturer: TOKAI HIT Co., Ltd. ������� �������� ������ ������ ������� ������� ������� ��������������� ������ ����������� ������ ������ ��������� ������ ������ �������� ������ ��������� ����������� ����������� ����������� ����������� ������������ ������� ���� ����������� ��������� ������ ������ ������ ������ ��������� ���� ���� � ����� ��������������� ������� ����������� ����������� ��������������� ������� ���������� ���������� ��� ����� ������ �������������������� ������� ��������������� � ��� ��� ����������� ��������� ��������� �������������� ��������� Hydraulic micro manipulator system �������� ����� ������� ����� ����� ����������� ������� ������ ������� ������ ������ ������ ��������� ������������� � � � This compact manipulation system features a suspension-type, soft-touch joystick. The hydraulic remote controls enable smooth, movement-free manipulation, minimizing needle deflection. Users can seamlessly switch between coarse and fine motion. Additionally, indicators on the coarse control mechanisms aid needle adjustments. � ������� ������ �������� ������� � Manufacturer: Narishige Co., Ltd. ���������������� Cameras for Microscopy � ����������� ������������ � Digital Sight Series ����������������� ��������� Cameras utilizing either PC-based control or standalone, touch-screen control units are available for use with the Ts2R. Configuration of Ts2R-FL+DS-Fi2+ DS-L3 *The optional camera port is required to attach the digital camera to the microscope. Please see the Digital Sight Series catalogue for more information and other products in the series. � � ����������� �������� ���������� ���� ������� ���������� ������������� ������ �������� ��� ������� � � � � � � � � � � ��������������� ������������������� ���������� ��������� ������ �������� ���� ������� � � � � � � �� �� � � ������� ��������� ������ ��������� ����������� �� � ������������� ��������� ��������� ��� ��������� ������� �� � � � ������� ������� ���������� �������� ������������ ������� � � � ���������� �������� ������������������� � �������� ��������� ���������� ������������� ��������� ���������� ���� ���� ������� ��������� ����� ��������� ������� � System Type ( C am era H e ads + Cont rol Unit s) ����� ������ ������������ ��������� ������������ ���������� ������������ ������������������� �������� ���������� ������� ��� ������� ������������ ��������������� ����������� ������������ ��������������� ���������� � � � ��������� ��������������� ��������� �������������� �������� ���������� ��� ������� �� �� ���������������� ��������� ������� ���� ��������� ���� ������ �������������������������� ������� �������������������������� ���� ���� ���������� ���� �������� ��� ���������� ���� ������������������������������� Features a high frame rate, 2.0-megapixel CCD. Displays SXGA live images (1600 x 1200 pixels max.) at 15fps (29fps max.). DS-Fi2 Camera Type Dimensions (Unit: mm) ��� ��� �� Color camera Monochrome Camera This 16.25-megapixel, high-definition camera for microscopes is equipped with Nikon’s digital SLR camera FX-format CMOS sensor. The DS-Ri2 provides superior color reproduction and fast frame rates. This 16.25-megapixel, high-definition camera for microscopes is equipped with Nikon's FX-format CMOS sensor. This monochrome CMOS camera enables high-sensitivity, low-noise imaging. DS-L3 Features a large touch panel display and variety of functions. This makes it possible to quickly and easily capture images without a computer or monitor. For PC-based control unit DS-U3 The DS-U3 control unit allows full control of the camera through a PC, from display=and capture to advanced processing and analysis. Flexibly accommodates a wide range of applications. ��� ��� Stand-alone control unit ��� ��� ��� ��� ��� ��� ���������������������� 6 ��� DS-Qi2 ����������� DS-Ri2 �� ��� ��� DS-Fi2 is a high-definition, 5-megapixel camera which delivers high-resolution images of up to 2560 x 1920 pixels and a frame rates up to 21 fps (Display mode: 1280 x 960 pixels). ��� High-speed, live display, color camera DS-Vi1 ����������� High-definition color camera ��� ��� ���������������������� 7 Ts2R / Ts2R-FL Specifications Optical System CFI 60 Infinity Optical System Observation method Brightfield, Apodized Phase Contrast*1, Phase Contrast, Nikon Advanced Modulation Contrast*2, DIC, Emboss Contrast*3 Illumination High luminescent white LED illuminator (Eco-illumination), Built-in Fly eye lens Diascopic illumination Brightfield, Apodized Phase Contrast*1, Phase Contrast, Nikon Advanced Modulation Contrast*2, DIC, Emboss Contrast*3, Epi-Fluorescence LED illuminator, built-in Fly eye lens, Can be configured with up to 4 different fluorescence LED units; available wavelengths:385, 455, 470, 505, 525, 590, 625 nm Episcopic illumination Tube • Binocular tube: Inclination: 35 degree Eyepiece(F.O.V.) 10x (22), 12.5x (16), 15x (14.5) • Ergonomic tube: Inclination:15-35 degree, Siedentopf type, Pupillary distance: 50-75mm Focusing Via nosepiece up/down movement, Stroke (manual): Up 8 mm, down 3 mm Coarse stroke: 5.0mm per rotation, Fine stroke: 0.1mm per rotation, Coarse motion torque adjustable, Refocusing mechanism mounted Nosepiece Sextuple nosepiece, With DIC prism slots Condenser Condenser turret, mount up to 7 modules: Phase Contrast, DIC, NAMC, IMSI, Emboss Contrast and ND for Bright Field Use with any one of ELWD condenser lens, LWD condenser lens and NAMC condenser lens Slider • Precentered or Centering PH Slider, 10x, 20x, 40x Objectives available for phase contrast • Emboss Contrast sliders(eyepiece-tube-side slider must be mounted), 10x, 20x, 40x, 60x objectives available for Emboss Contrast Stage • Plain Stage, Stage Size 260(X)×300(Y)mm • Rectangular Mechanical Stage Stroke: 114(X)×73(Y)mm, Adjustable XY stroke limit, Accepts 8 types of micro-testplate, well clamper and stage clip Holder • C-S-HP35 Petridish Holder 35mm • C-S-HLP100 Petridish Holder 100mm • C-S-HT Terasaki Holder for Terasaki holder and ø65 dish • C-S-HS Slide Glass Holder for glass slides, ø54 dish and hemocytometer • C-S-HU Universal Holder for Terasaki plate holder, glass slide, ø35-65 dish and hemocytometer • C-S-HG Glass Ring Holder • C-S-HLS Ring Holder Set • TC-S-HA Acrylic Holder Epi-fluorescence filter turret (with main body), Filter cubes with noise terminator mechanism Configure with up to 4 Epi-fluorescence filter cubes, one position is used during brightfield observation, Attachable Contrast Shield (optional; LWD,ELWD) Epi Fluorescence attachment Dimensions 286(W)×495(D)×542(H)mm 286(W)×495(D)×542(H)mm Weight (approx.) 17kg 18kg Rated Voltage/Electric Current 100V−240V, Less than 0.65A Power Consumption 30W *1 APC (Apodized Phase Contrast) is a type of phase contrast observation with reduced halo, thanks to Nikon’s unique lens coating. *2 NAMC (Nikon Advanced Modulation Contrast) is Nikon’s unique modulation contrast observation method which provides stereoscopic images similar to DIC observation, even with samples on plastic dishes. *3 Emboss contrast is Nikon’s unique contrast observation method. It provides pseudo-three-dimensional images using focal illumination, which gives high contrast to samples. Related products Fits in Every Laboratory — Simple to Use & Compact The new Inverted Routine Microscope ECLIPSE TS2 offers brilliantly clear images, enabling more efficient cell culture observation. Inverted Research Microscope with an excellent cost-performance ratio ECLIPSE Ti-S provides an excellent base platform for accommodating a variety of research applications. Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. January 2016 ©2016 NIKON CORPORATION N.B. Export of the products* in this catalog is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedures shall be required in case of export from Japan. *Products: Hardware and its technical information (including software) NIKON CORPORATION Shinagawa Intercity Tower C, 2-15-3, Konan, Minato-ku, Tokyo 108-6290, Japan phone: +81-3-6433-3705 fax: +81-3-6433-3785 http://www.nikon.com/products/microscope-solutions/ NIKON INSTRUMENTS INC. 1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A. phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only) fax: +1-631-547-0306 http://www.nikoninstruments.com/ NIKON INSTRUMENTS EUROPE B.V. Tripolis 100, Burgerweeshuispad 101, 1076 ER Amsterdam, The Netherlands phone: +31-20-7099-000 fax: +31-20-7099-298 http://www.nikoninstruments.eu/ NIKON INSTRUMENTS (SHANGHAI) CO., LTD. CHINA phone: +86-21-6841-2050 fax: +86-21-6841-2060 (Beijing branch) phone: +86-10-5831-2028 fax: +86-10-5831-2026 (Guangzhou branch) phone: +86-20-3882-0550 fax: +86-20-3882-0580 NIKON SINGAPORE PTE LTD. NIKON UK LTD. 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