Nikon ECLIPSE Ts2R Inverted Research Microscope Manual

Below you will find brief information for ECLIPSE Ts2R, ECLIPSE Ti-S. The ECLIPSE Ts2R is a compact, inverted microscope suitable for brightfield, phase contrast and epi-fluorescence observations. Its compact body fits easily inside laminar flow hoods, while providing stable sample observations. The optional mechanical stage enables observation of entire well plates.

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Inverted Research Microscope ECLIPSE Ts2R
Inver ted Research Microscope
A compact inverted microscope for your basic research needs
Compact body
Easy to work with
Newly developed mechanical stage option
Simple operations
Control buttons on the Ts2R microscope are intuitively located for a
streamlined workﬂow. The on/off and diascopic/epi-ﬂuorescence
switching controls are located on the front panel while buttons
pertaining to
either diascopic or
DIA illumination
epi-ﬂuorescence
brightness
control are zoned
adjuster
to the left and
FL illumination
brightness adjuster
right sides of the
microscope body,
Illumination
respectively.
LED ON/OFF
EPI illumination
DIA illumination
switch
switch
switch
Compact body for streamlined workﬂow
Easily ﬁts inside laminar ﬂow hoods
Illumination modules including the epi-ﬂuorescence light source
have been seamlessly incorporated into the microscope main body,
resulting in a compact and simple design form that's also durable.
The compact structure is also vibration-resistant to provide highly
stable sample observations.
The Ts2R can be conﬁgured with
an optional rectangular mechanical
stage. This stage provides a long
travel stroke, enabling users
to observe an entire well plate
from end-to-end. You can also
set limits to the travel stroke
(three-way) to match frequently
used samples or vessels. The
stage handle is offered in two
lengths, long or short, to further
accommodate the user's needs.
Epi-ﬂuorescence LED Light Source
The compact size of the Ts2R microscope makes it easier to store
and operate inside a laminar ﬂow hood. Additionally, the low stage
and side-port camera
position reduce user
fatigue from repetitive stage
manipulation and provides
clear visibility of the stage
and sample even with the
hood sash lowered.
Camera Port
A stage with the handle
attached to the left side is
also available.
Fluorescence Filter Cube Turret
New ergonomic stage design for improved workﬂow
1
The Ts2R’s stage height has been lowered by
approximately 30% compared to the standard
research microscope*, thereby ensuring a
comfortable hand position during repetitive
operation and sample exchange. Moreover
by lowering the support columns
and positioning the camera port
on the side of the tube, sample
visibility is improved.
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3
4
High precision and quality
5
6
Stroke adjustment using the XY
limit pin (three way)
*Comparison with Nikon’s Inverted
Research Microscope ECLIPSE Ti.
1
2
3
4
5
6
Slide Glass Holder
Petri Dish Holder 35mm
Petri Dish Holder 100mm
Well Plate Clamper
Terasaki Holder
Universal Holder
Advanced-optical performance
The Ts2R is compatible with Nikon's acclaimed CFI60 objective lenses
which provide high numerical apertures and long working distances to
deliver stunningly clear images.
High performance and quality optical accessories
Versatile applications with new LED illuminators
The high-intensity LED light source enables you to perform a wide
variety of observation methods similar to the full-size, inverted
research microscope ECLIPSE Ti. The Ts2R-FL model, which
offers epi-ﬂuorescence observation, provides four ﬂuorescence
channels and 7 different wavelengths to choose from.
2
DIA
FL
Brightﬁeld
DIC
APC (Apodized Phase Contrast)
Optical accessories achieve the same performance level as Nikon's
inverted research microscope ECLIPSE Ti, providing exceptionally
clear, sharp images.
Emboss Contrast
Phase contrast
NAMC (Nikon Advanced Modulation Contrast)
Epi-ﬂuorescence
Diascopic illumination
model
Diascopic and epi-ﬂuorescence
illumination model
3
Do more than before
DIA
Diascopic observations with enhanced quality
High-intensity LED Eco-illumination
Application
Do more than before
FL
Fluorescence images with uniform bright illumination
Application
Accurately reproduce illumination power every time
Nikon's LED Eco-illuminator is environmentally friendly with its low
power consumption and yet provides extremely bright illumination,
suitable for phase contrast and DIC imaging. The built-in ﬂy-eye lens
ensures uniform brightness across the entire ﬁeld of view. Furthermore,
LED excitation has no unwanted UV component, thereby eliminating
UV-mediated cell damage and improving cell survival rates during
long-term imaging.
The illumination power previously deﬁned by the user is replicated
when the same wavelength is used again, thus eliminating the need
for manual
adjustment of
LED Unit
light intensity
Filter Cube
when switching
between
wavelengths.
Phase contrast observation
Phase contrast is an optical contrasting technique that typically utilizes
a phase contrast objective lens and condenser annulus. The use of
a high-intensity LED light source results in clear images even at high
magniﬁcations.
High S/N epi-ﬂorescence observation in bright rooms
10x
40x
Apodized Phase Contrast (APC) observation
APC observation is a type of phase contrast microscopy which
minimizes unwanted halos in thick specimens. For example, APC
technique provides clearer details of thick samples such as dividing cells.
New Contrasting Technique, &quot;Emboss Contrast&quot;
Overlapping image with three colors with use of Imaging Software NIS-Elements
Nikon's new contrasting technique is compatible with both plastic and glass
culture dishes. Unlike phase contrast or NAMC, Emboss Contrast does not
require special objective lenses and therefore has minimal effect on epiﬂuorescence observation. Emboss Contrast allows thick samples such as
embryos to be easily observed in pseudo-three-dimensional image with
great clarity.
Multicolor ﬂuorescence observation
Using four different LEDs, multicolor ﬂuorescence observation can be easily
and efﬁciently achieved.
Image courtesy of Hideaki Watanabe, Ph.D. and Hisataka Hasegawa, Ph.D.
Spindle Observation System (optional, Released in April 2016)
Accurate observation of spindle bodies is easily attained with the Ts2R. The
system offers ﬁnely detailed work without damaging the spindle body.
Nikon Advanced Modulation Contrast (NAMC)
NAMC provides high relief, DIC-like
images of samples on plated on plastic
dishes, which is not possible with DIC
observation. Ts2R provides high-quality
NAMC images like Nikon's inverted
research microscope, ECLIPSE Ti.
4
The new Contrast Shield accessory (optional) blocks room light,
providing an easy and cost-effective method for achieving high signalto-noise ﬂuorescence observation in a brightly lit laboratory.
OPEN
OPEN
CLOSE
CLOSE
Differential Interference Contrast (DIC) observation
DIC provides high-resolution pseudo-three
dimensional images that have a shadowcast appearance. New high-intensity LED
illumination results in vivid DIC images even
at high magniﬁcations.
Fiber illumination
High signal-to-noise
ﬂuorescence imaging
Clear, vivid observation across
the entire ﬁeld of view
Noise Terminator helps to capture
vivid images.
The ﬂy-eye lens delivers uniform
brightness to the entire ﬁeld of view.
As an alternative to the built in LED system, an
external light source can be utilized through
the liquid light guide port to accommodate
users who require custom lighting needs.
5
Accessories
System Diagram
Thermo Plate TPX
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Thermo plate TPX provides accurate
and stable temperature control for the
specimen from room temperature to 50
degrees Celsius. Proprietary treatment
methods ensure that the glass surface of
the Termo Plate is breakage-free.
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Stage-top incubator
A stage-top incubation chamber can be utilized to accurately
control temperature, humidity and CO2 levels to maintain
optimal cell health during
long-term observation.
Manufacturer: TOKAI HIT Co., Ltd.
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Hydraulic micro manipulator system
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This compact manipulation system features a suspension-type, soft-touch joystick. The hydraulic remote controls enable smooth, movement-free manipulation,
minimizing needle deﬂection. Users can seamlessly switch between coarse and
ﬁne motion. Additionally, indicators on the coarse control mechanisms aid needle
adjustments.
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Manufacturer: Narishige Co., Ltd.
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Cameras for Microscopy
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Digital Sight Series
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Cameras utilizing either PC-based control or standalone, touch-screen control units are
available for use with the Ts2R.
Conﬁguration of
Ts2R-FL+DS-Fi2+
DS-L3
*The optional camera port is required to attach the digital camera to the microscope. Please
see the Digital Sight Series catalogue for more information and other products in the series.
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System Type ( C am era H e ads + Cont rol Unit s)
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Features a high frame rate, 2.0-megapixel
CCD. Displays SXGA live images (1600 x
1200 pixels max.) at
15fps (29fps max.).
DS-Fi2
Camera Type
Dimensions
(Unit: mm)
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Color camera
Monochrome Camera
This 16.25-megapixel,
high-deﬁnition camera for
microscopes is equipped with
Nikon’s digital SLR camera
FX-format CMOS sensor. The
DS-Ri2 provides superior
color reproduction and fast
frame rates.
This 16.25-megapixel,
high-definition camera for
microscopes is equipped
with Nikon's FX-format
CMOS sensor. This
monochrome CMOS camera
enables high-sensitivity,
low-noise imaging.
DS-L3
Features a large touch panel display and
variety of functions. This makes it possible to
quickly and easily capture images without a
computer or monitor.
For PC-based control unit
DS-U3
The DS-U3 control unit allows full control of
the camera through a PC, from display=and
capture to advanced processing and
analysis. Flexibly accommodates a wide
range of applications.
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Stand-alone control unit
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DS-Qi2
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DS-Fi2 is a high-deﬁnition, 5-megapixel
camera which delivers high-resolution
images of up to 2560 x
1920 pixels and a frame
rates up to 21 fps
(Display mode:
1280 x 960 pixels).
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High-speed, live display,
color camera DS-Vi1
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High-deﬁnition color camera
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7
Ts2R / Ts2R-FL Speciﬁcations
Optical System
CFI 60 Inﬁnity Optical System
Observation method
Brightﬁeld, Apodized Phase Contrast*1,
Phase Contrast, Nikon Advanced Modulation
Contrast*2, DIC, Emboss Contrast*3
Illumination
High luminescent white LED illuminator (Eco-illumination), Built-in Fly eye lens
Diascopic illumination
Brightﬁeld, Apodized Phase Contrast*1, Phase Contrast, Nikon Advanced Modulation
Contrast*2, DIC, Emboss Contrast*3, Epi-Fluorescence
LED illuminator, built-in Fly eye lens, Can be conﬁgured with up to 4 different
ﬂuorescence LED units; available wavelengths:385, 455, 470, 505, 525, 590, 625 nm
Episcopic illumination
Tube
• Binocular tube: Inclination: 35 degree
Eyepiece(F.O.V.)
10x (22), 12.5x (16), 15x (14.5)
• Ergonomic tube: Inclination:15-35 degree, Siedentopf type, Pupillary distance: 50-75mm
Focusing
Via nosepiece up/down movement, Stroke (manual): Up 8 mm, down 3 mm
Coarse stroke: 5.0mm per rotation, Fine stroke: 0.1mm per rotation, Coarse motion torque adjustable, Refocusing mechanism mounted
Nosepiece
Sextuple nosepiece, With DIC prism slots
Condenser
Condenser turret, mount up to 7 modules: Phase Contrast, DIC, NAMC, IMSI, Emboss Contrast and ND for Bright Field
Use with any one of ELWD condenser lens, LWD condenser lens and NAMC condenser lens
Slider
• Precentered or Centering PH Slider, 10x, 20x, 40x Objectives available for phase contrast
• Emboss Contrast sliders(eyepiece-tube-side slider must be mounted), 10x, 20x, 40x, 60x objectives available for Emboss Contrast
Stage
• Plain Stage, Stage Size 260(X)&times;300(Y)mm • Rectangular Mechanical Stage Stroke: 114(X)&times;73(Y)mm,
Adjustable XY stroke limit, Accepts 8 types of micro-testplate, well clamper and stage clip
Holder
• C-S-HP35 Petridish Holder 35mm
• C-S-HLP100 Petridish Holder 100mm
• C-S-HT Terasaki Holder for Terasaki holder and &oslash;65 dish • C-S-HS Slide Glass Holder for glass slides, &oslash;54 dish and hemocytometer
• C-S-HU Universal Holder for Terasaki plate holder, glass slide, &oslash;35-65 dish and hemocytometer
• C-S-HG Glass Ring Holder
• C-S-HLS Ring Holder Set
• TC-S-HA Acrylic Holder
Epi-ﬂuorescence ﬁlter turret (with main body), Filter cubes with noise terminator mechanism
Conﬁgure with up to 4 Epi-ﬂuorescence ﬁlter cubes, one position is used during brightﬁeld observation, Attachable Contrast Shield (optional; LWD,ELWD)
Epi Fluorescence attachment
Dimensions
286(W)&times;495(D)&times;542(H)mm
286(W)&times;495(D)&times;542(H)mm
Weight (approx.)
17kg
18kg
Rated Voltage/Electric Current
100V−240V, Less than 0.65A
Power Consumption
30W
*1 APC (Apodized Phase Contrast) is a type of phase contrast observation with reduced halo, thanks to Nikon’s unique lens coating.
*2 NAMC (Nikon Advanced Modulation Contrast) is Nikon’s unique modulation contrast observation method which provides stereoscopic images similar to DIC observation,
even with samples on plastic dishes.
*3 Emboss contrast is Nikon’s unique contrast observation method. It provides pseudo-three-dimensional images using focal illumination, which gives high contrast to samples.
Related products
Fits in Every Laboratory — Simple to Use &amp; Compact
The new Inverted Routine Microscope ECLIPSE TS2
offers brilliantly clear images, enabling more
efﬁcient cell culture observation.
Inverted Research Microscope
with an excellent cost-performance ratio
ECLIPSE Ti-S provides an excellent base platform for
accommodating a variety of research applications.
Speciﬁcations and equipment are subject to change without any notice or obligation on the part of the manufacturer. January 2016 &copy;2016 NIKON CORPORATION
N.B. Export of the products* in this catalog is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedures shall be required in case of export from Japan.
*Products: Hardware and its technical information (including software)
NIKON CORPORATION
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phone: +81-3-6433-3705 fax: +81-3-6433-3785
http://www.nikon.com/products/microscope-solutions/
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phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only)
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Key features

Compact and vibration-resistant design
Optional rectangular mechanical stage
Epi-fluorescence LED light source
Lowered stage height for ergonomic operation
Compatible with CFI60 objectives
Versatile LED illuminators

Frequently asked questions

The Ts2R supports Brightfield, Apodized Phase Contrast, Phase Contrast, Nikon Advanced Modulation Contrast, DIC, and Emboss Contrast.

Yes, the compact body of the Ts2R easily fits inside laminar flow hoods.

The Ts2R is compatible with Nikon's acclaimed CFI60 objective lenses.

The Ts2R-FL model provides four fluorescence channels with 7 different wavelengths to choose from.

Emboss Contrast is Nikon's unique contrasting technique that provides pseudo-three-dimensional imaging without requiring special objective lenses.

What observation methods are supported by the Ts2R?

Can the Ts2R be used inside a laminar flow hood?

What type of objective lenses are compatible with the Ts2R?

How many fluorescence channels are available on the Ts2R-FL model?

What is Emboss Contrast?

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