Thermo cytoperm 2 Gassed Incubator Operating instructions

The Thermo cytoperm 2 is a gassed incubator designed for cell and tissue cultures. It offers precise control and regulation of temperature, CO2 concentration, O2 concentration (optional), and relative humidity. This incubator allows for the creation of a controlled environment that closely mimics the physiological conditions needed for optimal cell growth.

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Document

GB

USA

cytoperm 2

Hot-Air Disinfectable Gassed Incubator

Operating instructions

Valid: 11.2006 / 50048320 B

Below is a list of the international Thermo marketing organizations.

Postal adress Germany Postal address USA

Thermo Electron LED GmbH

D – 63505 Langenselbold

Robert-Bosch-Straße 1

Enquiries from Germany

Telefon

Vertrieb 0800 1 536376

Service

Fax

0800 1 112110

Vertrieb/Service 0800 1 112114

E-Mail [email protected]

Thermo Electron Corporation.

275 Aiken Road

Asheville, NC 28804

USA

Enquiries from North America

Phone + 1 800-879 7767

Fax + 1 828-658 0363

E-Mail [email protected]

Enquiries from Europe, Middle East

and Africa

Tel. + 49(0) 6184 / 90-6940

Fax + 49(0) 6184 / 90-7474

E-Mail

Enquiries from Latin America

Phone + 1 828-658 2711

Fax + 1 828-645 9466

E-Mail [email protected]

[email protected]

Enquiries from Asia Pacific

Phone + 1 852-2711 3910

Fax + 1 852-2711 3858

E-Mail [email protected]

Internet: www.thermo.com

Copyright © Thermo Electron GmbH, 63505 Langenselbold, Germany

No part of this publication may be reproduced or transmitted in any form or by any means, including photocopying and recording, without the written permission of Thermo Electron.

Various sections of this instruction manual may be copied only for in-house use by the equipment operator, e.g. to provide employees with instructions on accident prevention measures.

Thermo Electron can accept no liability or responsibility for the marketability or the suitability of this instruction manual for a certain purpose other than that specified under "Areas of application of equipment".

Thermo Electron reserves the right to change the content of this instruction manual at any time and without prior notice.

As regards foreign-language translations, the German version of this instruction manual is binding.

This edition of the instruction manual applies to the cytoperm 2 unit specified on the front page.

Date of publication: November, 2006

Nominal charge

The safety concerning the protection of persons, environment and material to be treated mainly depends on the behaviour of the operating personnel of these units.

Please read and observe the following instructions carefully before starting the unit in

order to avoid faults and resulting damage, especially adverse health effects.

LIST OF CONTENTS PAGE

1. GENERAL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5/7

Explanation of icons in the instructions manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Explanation of icons in the short instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

General instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Equipment log book. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2. AREAS OF APPLICATION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

3. SAFETY INSTRUCTIONS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

4. SETUP AND INSTALLATION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

10-12

Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Place of installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Stacking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Clearances. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Supply connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Mains connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Gas supply connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

RS 232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

"Potential-free contact" connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

5. DESCRIPTION OF THE UNIT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

13/14

Levelling the shelves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Functional principle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Heating system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Gassing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Humidification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Door switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Internal fittings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Six-piece Gas-tight glass screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Lockable outside door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Disinfection routine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

"Potential-free contact" connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

6. OPERATE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

15-30

Switch panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Water supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Mains switch (key-operated switch). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Temperature protection device (temperature limit controller). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Control and display panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18-21

Disinfection routine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21/22 auto-start routine / auto-zero routine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Error code enquiry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Error codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25/26

Switching functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Switching function: Acoustic alarm ON/OFF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Switching function: Humidification ON/OFF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Switching function: Manual zero calibration of CO

measuring system. . . . . . . . . . . . . . . . . . . . . . . . . . 29

Switching function: Setpoint lock-in ON/OFF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Switching function: Door heater ON/OFF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

50 048 320 3/45

LIST OF CONTENTS PAGE

7. START-UP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8. OPERATION

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Guidelines for use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Before putting the unit into operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

During operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Operation interruptions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

9. SHUTDOWN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

10. MAINTENANCE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Cleaning/Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Equipment log book. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Replacing of electrical parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

11. AUTHORIZED EQUIPMENT PARTS AND ACCESSORIES. . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

12. TECHNICAL SPECIFICATIONS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

13. MATERIALS USED. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

14. GAS CONSUMPTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

15. PH-VALUE OF CULTURE MEDIA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Basic rules of sound microbiological engineering practice. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

General rules. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Handling of pathogenes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43/44

Handling of human- and animal-pathogenic biological agents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

APPENDIX

Statement of conformity

Log Book

Explanation of confirmation of safety

Information request form regarding maintenance and servicing contract

Communication protocoll of RS 232 device

4/45 50 048 320

1. GENERAL

EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL

In chapters of the instruction manual which deal with safety, this icon appears under the title of the chapter. Displayed on the equipment, this icon denotes that special attention must be paid to the information given in the instruction manual or accompanying documents.

Marks information in the instruction manual for optimizing use of the equipment

"Water supply" vent/overflow aqua dest

0 des start/ stop

"des" i control auto-start auto-zero des

"Add/drain water" quick release coupling

Unit ON

Unit OFF

"Disinfection mode" key-operated switch

Marks information for higher surface temperature in the disinfection mode

Overtemperature protection, temperature limit controller

Selection of switching/interlocking functions

Error code enquiry / acknowledgment of "acoustic signal"

"Change switching functions activated" display

Automatic startup of unit and calibration of measuring systems

"auto-zero" calibration active display

"Disinfection mode" display

Information on proper disposal / recovery of raw materials

50 048 320 5/45

2. AREAS OF APPLICATION

EXPLANATION OF ICONS TO THE SHORT INSTRUCTIONS

Switch on unit (operate the main switch) max.

aqua dest.

min.

> + 2 °C

°C

Check the water level

Set the temperature limit controller

Close unit doors

≥ 1 sec auto-start des start/ stop

Read the operating instructions

Start "disinfection routine" automatical "auto-start"

≥

5 s & start / stop

°C +

%CO2 +

%O2 +

%rH +

≥

30 s

auto-start auto-start

180 °C

Open unit doors for at least 30 seconds

Set the desired setpoints

"activate" the auto-start

End of the auto-start, bring in the samples

> 30 s

Take out the samples, open unit doors for at least

30 seconds

Flow chart of disinfection routine t

6/45 50 048 320

1. GENERAL

General instructions

To avoid errors and causing damage, especially personal injuries, be sure to read this manual carefully before using the equipment, and follow all instructions.

When setting up and operating the unit, be sure to comply with the instructions contained in this manual as well as all applicable laws, regulations and directives in your country (FRG: ZH 1/119,

ZH 1/342, ZH 1/343, ZH 1/598).

The unit meets the following safety requirements x x

DIN 12880 Part 1/11.78

DIN VDE 0700 Part 1 (IEC 335-1; EN 60335-1/11.90) x EN 61010

When you have an enquiry, order replacement parts or file a complaint, please state the data on the nameplate and, if applicable, the fault code.

Equipment log book

We advise you to keep an equipment log book.

Keep a record of inspections and testing, calibration work and any major work carried out on the unit - such as maintenance, agents loaded, etc. - in this log book (refer to APPENDIX).

Operating instructions

The operator (employer) is expected to provide anyone who works on or with the equipment with

written instructions, based on this instruction manual, for the tasks to be performed. These instructions should be easy to understand and in the language of the persons operating the equipment.

This also applies to disinfecting and cleaning the unit (also refer to Chapter 10: Maintenance).

50 048 320 7/45

2. AREAS OF APPLICATION x x x x

The cytoperm 2 gassed incubator is an item of laboratory equipment for cell and tissue cultures and can be used to simulate the physiological environmental conditions of cells.

The unit is generally suitable for setup and operation in the following areas:

In laboratories for microbiological and biotechnological work.

Medical and microbiological laboratories to DIN 58956.

Central laboratories in clinics and hospitals.

Laboratories operating at safety levels L1, L2 and L3.

Only organisms which comply with the requirements for safety levels L1, L2 and L3 may be handled in the unit.

The unit must be set up and operated in accordance with all applicable regulations in your country.

(FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598)

It is not permitted to handle gases or agents whose vapours are combustible or can form a hazardous, potentially explosive atmosphere when mixed with air.

Be sure to comply with the applicable regulations in your country.

(FRG: ZH 1/10)

8/45 50 048 320

3. SAFETY INSTRUCTIONS x x x x x x x

In the case of biological incubators, biological safety with regard to the protection of persons, its surroundings and the load is heavily dependent on the observance of the applicable regulations by the persons using the equipment.

Even then, however, the possibility of hazards, especially health hazards, arising cannot be ruled out.

The residual risk depends on the work performed in each individual case.

To avoid errors and causing damage, especially personal injuries, be sure to carefully read this manual before putting the equipment into operation, and follow all instructions.

Safe and reliable operation of the unit can only be guaranteed if the necessary inspections, maintenance and repair work are carried out by Heraeus Service Department personnel or by personnel authorized by our company.

Gas is to be supplied to each unit by means of a pressure reducer with the inlet pressure set to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons

(FRG: ZH 1/119).

The place of installation must be thoroughly ventilated in order to expel the gases escaping around the pressure relief valve to the outside.

To maintain the temperature protection function, the functioning of the overtemperature protection device, temperatur limit controller must be checked at reasonable intervals.

Only original replacement parts authorized by the manufacturer are to be used.

Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEER-

ING PRACTICE".

50 048 320 9/45

4. SETUP AND INSTALLATION

When setting up, installing and operating the unit, be sure to comply with all applicable regulations in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598)

Transport

Handle the unit with care during transport. Do not lift up the unit by its door.

Refer to TECHNICAL SPECIFICATIONS for weights and dimensions.

Place of installation

The place of installation must be dry and draught-free.

The unit should not be placed or operated in recesses which cannot be ventilated.

To avoid or at least minimize deviations from the technical specifications, the temperature at the place of installation must be in the range +18°C to +30°C.

Set up the unit on a solid, non-flammable surface (laboratory tables, subframes).

Make sure that the unit is in a stable and perfectly upright position.

Do not cover or obstruct the fresh air or exhaust vents in the unit housing

(please observe the minimum clearances - see page 11).

, O

or N

are admitted into the gassed incubator. CO

is a health risk where as O

is a fire

hazard. The place of installation must therefore be well-ventilated.

Gases discharged from the pressure relief valve must be safely expelled outside by means of the ventilation systems.

It may be necessary to take special measures in order to ensure proper ventilation when several units are installed in the same room.

For information on gas flow during operation, refer to the APPENDIX.

Stacking

The unit is not suitable for stacking when operated in the manner intended.

10/45 50 048 320

5. DESCRIPTION OF THE UNIT

Clearances

When setting up the unit, the minimum clearances between the unit and adjacent surfaces and other units must be maintained.

Larger clearances are recommended to facilitate accessibility for installation and the supply connections.

100 150 100

Fig. 1: Minimum clearances in mm

Supply connections

Mains socket

2 gas connection

/ N

2 gas connection (option)

Label:

Supply connections

Potential-free contact

RS 232 interface

Fig. 2: Supply connections (rear panel of unit)

Mains connection

Prior to connecting the unit to the mains, make sure that the power supply ratings match those stated on the nameplate.

Your unit is equipped with a power supply cable with a grounding contact-type plug.

Fuse protection required: x Use a type G 16 circuit-break switch or a type T 16 A fusible cutout.

Refer to the Chapter titled TECHNICAL SPECIFICATIONS for unit power consumption.

50 048 320 11/45

4. SETUP AND INSTALLATION

Gas supply connection

The gas connections are located on the back of the unit.

The gas must be supplied to each unit via a pressure reducer with the inlet pressure set to between 0.8 and max. 1 bar.

This setting must not be changed for safety reasons

(FRG: ZH 1/119).

The gases must have at least 99.5% purity.

If several units are placed in the same room, special ventilation measures are required.

x x

gassing

Connect the gas cylinder to the gas nozzle on the unit.

/ N

gassing (optional)

If you intend to run the unit with an oxygen content above 21%, connect an oxygen cylinder.

If you intend to run the unit with an oxygen content less than 21%, connect a nitrogen cylinder.

To deactivate the oxygen control, set the nominal value to 21.0% (average oxygen content of ambient atmosphere).

RS 232 interface

Serial interface for computer-aided acquisition and documentation of critical operating parameters

(temperature, CO

, O

, relative humidity, error codes, etc.). The RS 232 interface can connect a single unit in combination with the "Kelvilog"



software package.

"Potential - free contact" connection

The "Potential-free contact" (1 changeover contact) is rated for the following circuits:

Circuit

Mains-operated circuits

SELV / SELV - E - circuits

(cf. VDE 0100 Part 410)

F1x

T2A

Voltage max. 250 V AC

25/50 V AC

60/120 V DC

Fuse to be installed by customer max. 6 A max. 2/1 A max. 1/0.5 A

H1x Kx

S1x

H2x Kx A1

View X

Fig. 3 : Application example of "Potential - free contact" connection

12/45 50 048 320

5. DESCRIPTION OF THE UNIT

Levelling the shelves

The shelves can be aligned with the aid of a spirit level with height-adjustable feet.

Functional principle

The atmosphere in the chamber can be regulated in relation to its temperature, CO

2 concentration, O

2 concentration (optional) and relative humidity.

Heating system

The temperature inside the unit can be regulated in the range of +7°C to +50°C, but must be at least +5°C (approx. +7°C for the O

2 version) above the ambient temperature of the unit.

Condensation on the glass door is largely prevented by heating the unit door. If the unit door is left open for a long period of time, however, condensation cannot be ruled out.

The unit door heater can be switched off as required (refer to Chapter headed OPERATION).

This enables the unit to be operated at high ambient temperatures or when the operating temperature is only supposed to exceed the ambient temperature by approx. +4°C (approx.

+6°C on the O

2 version).

Example of CO

2 version:

Ambient temperature

+25°C

+24°C

+32°C to +33°C

Operating temp.

+37°C

+28°C

+37°C

Heating of unit door

OFF

Gassing

Connections to the gas supplies (CO

, O

or N

) are located on the rear panel of the incubator.

x The CO

20%.

content of the atmosphere in the chamber can be regulated in the range 0% to x The O

concentration inside the unit can be regulated in the range 3% to 90% O

by admitting N

(< 21% O

) or O

(> 21% O

x The sum of the nominal values for CO

+ O

must not exceed 90%.

Example: 10% CO

+ 80% O

= 90% (possible)

20% CO

+ 80% O

= 100% (impossible)

Before entering the chamber, all gases pass through a filter where particles larger than 0.3 µm are retained. Filter efficiency is 99.998%.

A fan integrated in the rear of the interior wall ensures that the gases and the incubator atmosphere are thoroughly mixed.

The sensors for CO

, O

and relative humidity are also located on the rear panel of the interior wall. These sensors measure the parameters inside the unit and transmit corresponding signals to the closed-loop controller.

A pressure compensation vent on the rear panel of unit avoids undesirable pressure build-up inside the unit during the admission of gases.

The place of installation must be thoroughly ventilated in order to expel the gases escaping around pressure compensation vent.

50 048 320 13/45

5. DESCRIPTION OF THE UNIT

Humidification

The atmosphere in the chamber is humidified through the admission of steam generated in a condenser. The steam is admitted into the chamber by way of a "pyrolytic germ barrier" (500°C).

Relative humidity (rH) can be regulated between 60% and 95%.

To ensure reliable operation of the steam generator, only fill the reservoir with fully desalinated or distilled water!

The capacity of the reservoir is approx. 4.7 ltr (difference between min. and max. levels:

2.2 ltr).

Door switch

The heating system, gas supply and humidifier are switched off when the unit door is opened.

The unit door can only be closed when all glass doors are sealed properly. If the unit door is not properly closed while the unit is in operation, a visual alarm is given (all LEDs flashing).

If the door is open for longer than 10 minutes, an acoustic alarm sounds when this monitoring function is activated by way of function level 1 (refer to Page 26).

Internal fittings

Pull-out shelves, with support brackets to prevent tilting, are arranged inside the units. The support can be offset in the carrier racks at intervals of 46 mm.

Six-piece gas-tight glass screen

By virtue of the small vent cross-section, unnecessary cooling and discharge of the atmosphere in the chamber is largely avoided when a glass door is opened of the six-piece gas-tight glass screen.

Lockable outside door

The outside door is lockable.

Disinfection routine

This routine is for disinfecting the interior of the unit. In this process, the unit is heated to 180°C and kept at the disinfection temperature for approx. 3 hours. The unit is then automatically cooled down again and the nominal values for temperature, gases and relative humidity are reset. The entire procedure lasts approx. 13 hours, after which the unit is ready for operation again.

"Potential - free contact" connection

Connection for the customer's own signalling system, e.g. telephone system, building services management systems.

If the overtemperature-protection device trips or an error is detected by the diagnosis system, a fault message is sent to the customer's own signalling system.

14/45 50 048 320

6. OPERATE

Switch panel

(1)

(2)

(A)

37.0

5.0

7.0

95.0

cytoperm 2 des autozero max min

(5) i i auto-start control

(6)

(A) "Control and display panel" (refer to Fig. 7)

(1) Indicator lamp "Fault, overtemp. protection"

(2) Indicator lamp "Disinfection mode"

(3) Indicator lamp "Master Switch"

(4) Vent/overflow

(5) Level gauge

"Water supply"

(6) Setting knob

(7) Key-op. switch

"Temperature protection device"

"Disinfection mode"

(8) Master switch (key-operated switch)

(9) Quick-release coupling "Add/drain water" des start/ stop (7)

(3)

(4)

Fig. 4: Switch panel

(8)

(9) aqua dest

50 048 320 15/45

6. OPERATE

Water supply

Level gauge (5) indicates the water level.

The water level should be in the range between "min and max". This range is equivalent to a volume of 2.2 ltr. If the water supply falls below the "min." level, a reserve of 0.5 ltr. is still available.

Filling the water reservoir

For humidification of the incubator, water of the following quality must be used:

demineralised water that has been sterilised.

sterile water that has been demineralised. Distilled or autoclaved water is suitable.

Fill the canister with the water and leave the screw cap off. Hang the canister on the bracket located on the upper left of the incubator. (fig 1) Connect the tubing connector (fig 1) into the quick fit coupling, the water reservoir will be filled under the force of gravity.

Should the water not flow, there may be an airlock in the tube, in which case press on the tube bulb to move the air through.

Attention:

If the reservoir is overfilled the excess water any will flow from the overflow pipe.

After filling place the filling canister on the floor and allow water in the filling tube to return to the canister before removing the quick fit connection. (fig 2)

Attention:

After filling completely empty and dry the filling canister, store the canister dry to prevent the formation of contamination.

Figure 1

1 6/45

Figure 2

50 048 320

6. OPERATE

As the outlet not only functions as an overflow but also as a vent, it must be unsealed while the unit is in operation.

To drain the empty filling canister, place it on the floor and connect it using the quickrelease coupling (9). Take care that the filling canister does not overflow. Disconnect the quick-release coupling if necessary.

Always drain the water reservoir before transporting the unit.

Mains switch

0 Unit OFF

I Unit ON (the green indicator lamp (3) indicates that unit is ready for operation).

Fig. 5: Mains switch (8)

50 048 320 17/45

6. OPERATE

Temperature protection device (temperature limit controller)

Fig.

6: Temperature

limit controller (6)

A temperature protection device is built into the unit and acts as a temperature limit controller. It conforms to Thermal Safety Class 3.1 to DIN 12880 Part 1/11.78.

In a functional state, the temperature limit controller assumes the control function if the set temperature is exceeded.

The red indicator lamp (1) "Fault" lights up as soon as the temperature limit controller responds.

The cutout temperature of the temperature limit controller can be adjusted in the range

+20°C to +55°C using a coin or screwdriver.

Have your temperature protection device tested for proper operation at reasonable intervals. This applies in particular before prolonged work processes.

Operational test:

Condition: Nominal temperature (controller) reached/constant.

To run this test, turn the dial on the Temperature Limit Controller so that it shows approx.

+5°C less than the temperature indicated on the temperature controller.

If the Temperature Limit Controller responds and the red indicator lamp (1) "Fault" comes on, the Temperature Limit Controller is operating properly.

Now set the Temperature Limit Controller to the required value depending on the max. cutout temperature. x If the Temperature Limit Controller is set to the upper temperature limit, it assumes the function of unit protection (protection of unit and surroundings).

x If the Temperature Limit Controller is set to approx. +2°C above the nominal temperature set at the temperature controller, it assumes the function of load protection (protection of unit, its environment and loaded material).

The load protection function is only effective above room temperature.

If "Fault" is displayed during operation: x Check the settings on the Temperature Limit Controller and the controller, and correct them if necessary. If the fault cannot be rectified, contact our service department.

Control and display panel (A) x x x x x x x

Microprocessor-controlled controller with digital temperature, CO

, O

(optional) and relative humidity displays.

The controller has the following functions:

Temperature "set, display and control"

concentration "set, display and control"

concentration "set, display and control" (OPTIONAL)

Relative humidity "set, display and control"

Error code "scan" auto-start function "activate"

Switching functions

- Acoustic alarm

- Humidification

"switch ON/OFF"

- Manual calibration of CO

measuring system "execute"

- Setpoint lock-in

- Door heater

"select ON/OFF

"switch ON/OFF"

18/45 50 048 320

6. OPERATE

(1)

(6)

(7)

(8)

(2)

(3)

(4)

(5)

37.0

5.0

7.0

95.0

%CO.

des autozero

(11)

(12)

(13)

(14)

(15)

(16)

(9) i i auto-start

(17)

(18)

(10)

control

(19)

(20)

Fig. 7: Control and display panel (A)

(1) Indicator lamp "HEATING"

(2) Display "Temperature"

(11) Indicator lamp "Disinfection mode"

(12) Key "Temperature setpoint"

(3) Indicator lamp "CO

GASSING"

(4) Display "CO

2 concentration"

(13) Indicator lamp "auto-zero"

(14) Key "CO

setpoint"

(5) Indicator lamp "O

GASSING" (optional) (15) Key "O

setpoint" (optional)

(6) Display

(7) Indicator lamp "HUMIDIFICATION"

(8) Display

concentration" (optional) (16) Key

"Relative humidity" (18) Key

"Relative humidity setpoint"

(17) Indicator lamp "auto-start"

"Error code inquiry" / Start

calibration routines

(9) Key

(10) Key

"Increase reading"

"Decrease reading"

(19) Indicator lamp "Control"

(20) Key "Switching functions"

50 048 320 19/45

7. START-UP

In its as-delivered condition, the unit is preset to the following values:

Temperature: 37.0°C

concentration: 0.0%

concentration: 21.0% (optional)

Relative humidity: 60.0%, humidity control activated via function level 2

Setting the temperature setpoint:

Instruction

Switch on unit

(master switch = " I ")

Display temperature setpoint

Entry / Keys / Remarks

All display elements come on for approx. 15 seconds.

- Auto-test of controller -

°C

Press

Select temperature setpoint

°C

Press

Display / Remarks / Status

Example:

Actual setpoint is displayed and decimal point flashes

Setpoint increases

Setpoint decreases

°C &

Press

Adopt NEW temperature setpoint

°C

Release

Temperature inside unit is displayed

Setting the CO

setpoint:

Instruction

Display CO

setpoint

Entry / Keys / Remarks

%CO

Press

Select CO

setpoint

%CO

& + Press

%CO

& Press

Adopt NEW CO

setpoint

%CO

Release

Display / Remarks / Status

Actual setpoint is displayed and decimal point flashes

Setpoint increases

Setpoint decreases

concentration inside unit is displayed

Setting the O

setpoint (optional):

Instruction

Display O

setpoint

Select O

setpoint

Entry / Keys / Remarks

Press

Display / Remarks / Status

Actual setpoint is displayed, decimal point flashes

Setpoint increases

& + Press

& Press

Setpoint decreases

Adopt NEW O

setpoint

Release

concentration inside unit is displayed

The control is deactivated when the unit is set for 21% O

since this corresponds to the O

content of air.

20/45 50 048 320

6. OPERATE

Setting the relative humidity setpoint:

Instruction

Display the relative humidity setpoint

Set the relative humidity setpoint

Entry / Keys / Remarks rH rH

Press

& + Press rH & Press

Adopt NEW relative humidity setpoint rH Release

Disinfection routine

Display / Remarks / Status

Actual setpoint is displayed and decimal point flashes

Setpoint increases

Setpoint decreases

Relative humidity concentration inside the unit is displayed

The entire interior of the unit together with all sensors can be disinfected with hot air at 180°C.

The disinfection routine lasts approx. 13 hours.

Start disinfection routine:

Instruction

Open the unit door for at least

30 seconds

Entry / Keys / Remarks

Remove samples and vessels from the interior of the unit

Activate the disinfection routine (turn key in direction of arrow) des start/ stop

Display / Remarks / Status

All displays flash

Indicator Lamp (page 18 (11)) flashes and indicator lamp (page 15

(3)) comes on.

Press for approx. 1 second

Close the door

"des"

Disinfection routine runs automatically.

2 and relative humidity displays go out;

Temperature Limit Controller is disabled.

2 display indicates the phases of the disinfection routine.

"auto-start" routine begins on completion of the disinfection routine .

In the disinfektion mode (appr. 13 h) occur slightly higher temperatures occur on the outer surface, the door area and the internal door grips. When LED "des" is flashing, proceed with particular caution.

Avoid touching the door areas during the disinfection routine.

50 048 320 21/45

7. START-UP

Temp.

180°C

Heating phase

Disinfection phase

Cooling phase

37°C

Time

Start

End

Flow chart of disinfection routine:

Status display on CO

display:

Heat:

The chamber is heated to 180°C; the disinfection temperature can be read off the temperature display.

Disinfect:

When the disinfection temperature of 180°C is reached, a disinfection phase with a duration of approx. 3 hours is started. If the door is opened during the period and the temperature drops below 180°C, the disinfection routine restarts automatically.

Cool:

On completion of the disinfection phase, the unit cools down to the originally set nominal temperature. x The yellow indicator lamp "des" as soon as the unit reaches the set operating temperature

(e.g. 37.0°C) is reached. The sensors are automatically calibrated (auto-start) and the unit is readjusted to the set gas / rel. humidity setpoints. The disinfection routine is completed.

x If a temperature of 200°C is exceeded during the disinfection routine, the latter is cancelled and all poles of the heating system are disconnected. Error message 501 is displayed.

Cancelling the disinfection routine:

Instruction

Cancel disinfection

Entry / Keys / Remarks des start/ stop

Display / Remarks / Status

The CO

2 display indicates the cooling phase of the disinfection routine.

Press for approx. 1 second

22/45 50 048 320

6. OPERATE auto-start routine / auto-zero routine

An auto-start routine / auto-zero routine will have to be run when putting the unit into operation or if the temperature setpoint is altered by more than 1°C.

To ensure that there is only air, i.e. no CO

in the chamber before the auto-start routine is activated, ventilate the chamber by opening all doors.

The auto-start can only be activated if the doors are open for at least 30 s.

The indicator lamp "auto-start" (page 18 (17)) indicates when the auto-start routine is running.

Calibration can take up to 5 hours, especially if the unit is cold.

If the unit door is opened during the auto-start, the auto-start routine / auto-zero routine restarts automatically after the unit door is closed.

On resumption of the power supply after a power failure, the auto-start routine / auto-zero routine is also restarted.

Display / Remarks / Status

All display segments flash

Instruction

Open unit doors for at least

30 seconds

Entry / Keys / Remarks

Adjust or check the setpoints

Activate auto-start

Refer to pages 19/20

+ & -

Indicator lamp (page 18 (17)) "autostart" flashes

Press for approx. 5 seconds

Close all unit doors Temperature reads "Actual value"

reads " 0.0"

reads "21.0" (optional) rel. humidity reads "Actual value" x x

The unit is set to the temperature and relative humidity setpoints. When temperature and relative humidity setpoints are achieved consistently, the CO

measuring system is automatically calibrated and the O

sensor (optional) is calibrated for 21%.

x Indicator lamp (page 18 (17)) "auto-start" goes out.

The auto-zero routine determines the reference value and O

sensor (optional) is calibrated.

Gas is admitted until the CO

setpoints are reached.

The auto-zero routine compensates for zero drift of the CO

measuring cell. The auto-zero routine runs automatically every 6 hours. When the indicator lamp (page 18 (13)) "auto-zero" is flashing, the auto-zero routine is in progress. If the door is opened or closed during the auto-zero routine,

auto-zero calibration is restarted.

If the auto-start has not been completed after 24 hours at the latest (page 18, indicator lamp (17)

"auto-start" is still flashing), the auto-start will have to be cancelled manually (refer to page 23) and repeat.

50 048 320 23/45

7. START-UP

Instruction

Cancel auto-start routine

Cancel auto-zero routine

Entry / Keys / Remarks

Press for approx. 5 seconds

Display / Remarks / Status

Indicator lamp (page 18 (17)) "autostart" goes out and auto-zero calibration routine starts

Indicator lamp (page 18 (13)) "autozero" flashes

Indicator lamp (page 18 (13)) "autozero" goes out

Press for approx. 5 seconds

Unit displays the current actual values

If a repeat auto-start / auto-zero routine is unsuccessful, contact the service department of

Heraeus Instruments.

Error code request

The unit is equipped with an error diagnosis system.

This diagnosis system lets you detect and evaluate a fault during operation by means of specific codes.

If an error occurs within a control loop, the relevant display flashes to indicate this.

Press ( page 18 (18)) to display the error detected by the diagnosis system and to acknowledge the alarm.

When the error is cleared, the error message is automatically closed and the display stops flashing.

Exception: Error 502

Instruction

Scan error code /

Acknowledge acoustic alarm

Entry / Keys / Remarks i Hold down

Display / Remarks / Status

Error code is displayed/acoustic alarm is acknowledged

Display " - - - ":

No error detected

24/45 50 048 320

6. OPERATE

ERROR CODES

Error condition POSSIBLE REMEDY CODE CAUSE

100

101

104

200

201

204

300

301

302

303

304

400

404

405 auto-start

≥

°C

10 min

Error during auto-start

Unit doors are open

Temperature below setpoint

Temperature above setpoint

Temperature sensor faulty

below setpoint

above setpoint faulty

measuring cell

below setpoint

above setpoint

below setpoint

above setpoint

sensor faulty

Measuring cell outside calibration range

Doors are open for more than 10 minutes

Actual value < setpoint

-0.5°C

Actual value > setpoint

+1°C

Actual O

+2%

Actual O

-2%

Actual O

+2%

value > setpoint

Gassing with N

value < setpoint

Gassing with O

value > setpoint

Gassing with O

Repeat the auto-start routine (refer to pages 22)

Close the unit doors

(refer to page 14) or just acknowledge acoustic alarm

Check the settings on the controller and Temperature Limit Controller

(refer to pages 17 and 19)

Ambient temperature could be too high: check the circuit status of the door heater (refer to pages 15 and

30)

Contact the service department of

Heraeus Instruments

Sensor broken/shortcircuited

Actual value < setpoint -1% Check the gas supply for

- cylinder content

- inlet pressure

- supply line and connection to unit

Refer to page 12

Actual value > setpoint +1% Check the gas supply for

- inlet pressure

Refer to page 12

Sensor broken/shortcircuited

Contact the service department of

Heraeus Instruments

Actual O

value < setpoint

-2%

Gassing with N

Check the gas supply for

- gas cylinder contents

- inlet pressure

- supply line and connection to unit

Refer to page 13/14

Check the gas supply for

- inlet pressure

Refer to page 13/14

Check the gas supply for

- gas cylinder contents

- inlet pressure

- supply line and connection to unit

Refer to page 13/14

Check the gas supply for

- inlet pressure

Refer to page 13/14

Contact the service department of

Heraeus Instruments

Relative humidity below setpoint

Relative humidity sensor faulty

Evaporator faulty

Actual value < setpoint -5%

95°C > Actual value

> 620°C

Check the water reservoir

Contact the service department of

Heraeus Instruments if necessary

Sensor faulty/short-circuited Contact the service department of

Heraeus Instruments

Contact the service department of

Heraeus Instruments

50 048 320 25/45

7. START-UP

500

501

502 des

"des" temperature below setpoint des

"des" temperature above setpoint

Actual value < 180°C -10°C

Actual value > 180°C +10°C des

Error during disinfection procedure

Power failure > 1 sec

Repeat the disinfection process

Contact the service department of

Heraeus Instruments if necessary

Contact the service department of

Heraeus Instruments

Press the des switch (Fig.4, (7)) to reset the error and repeat the disinfection procedure; check voltage supply for power failure

A malfunction of the unit is not the only explanation for error codes 101, 201, 303. They can also be displayed after a setpoint is decreased.

Example:

When operating the unit with the setpoint entered for 10% CO

If the CO

setpoint is decreased to 5% CO

, the unit cannot reach this lower CO

concentration quickly enough due to its tightness. Error 201 is displayed.

The designated error messages are displayed when: x Setpoint << actual value.

x Setpoint temperatur < ambient temperature + 7°C

To avoid this, open the doors for a short time. This also applies to the reduced temperature and

setpoints.

If these measures are unsuccessful or if errors with undefined codes occur, please notify our service department (refer to Appendix).

When requesting our service, please state the scanned error code.

26/45 50 048 320

6. OPERATE

Switching functions x x x x x

Press (page 18 (20)) to select the individual switching functions as required.

The indicator lamp "control" (page 18 (19)) indicates when one of the switching functions specified below is selected:

Acoustic alarm ON/OFF

Humidification ON/OFF

Manual zero calibration of CO

measuring system

Setpoint lock-in ON/OFF

Door heater ON/OFF

Switching function: acoustic alarm ON/OFF

Factory setting: acoustic alarm switched on.

Instruction

Select function level 1

Entry / Keys / Remarks

Press

Circuit status display and factory setting:

Acoustic alarm ON

Change circuit status: acoustic alarm OFF

Change circuit status: acoustic alarm ON

Exit function level

or Press

Release and press again

Press

Release

Press any setpoint key

Display / Remarks / Status

Indicator lamp "control" flashes and current function level is indicated by a flashing display

°C

Actual temperature, CO

, O

and rel. humidity values are displayed again after 1 minute

Actual temperature, CO

, O

and rel. humidity values are displayed again immediately

50 048 320 27/45

7. START-UP

Switching function: Humidification ON/OFF

Factory setting: Humidification switched on (set to 60 % rel. humidity).

Instruction

Select function level 2

Display circuit status, factory setting:

Humidification ON

Change circuit status:

Humidification OFF

Change circuit status:

Humidification ON

Exit function level

Entry / Keys / Remarks

Hold down

or Press

Release and press again

Press

Press any setpoint key

Display / Remarks / Status

Indicator lamp "control" flashes and current function level is indicated by the flashing display

°C

Actual temperature, CO

, O

and rel. humidity values are displayed again after 1 minute

Actual temperature, CO

, O

and rel. humidity values are displayed again immediately

28/45 50 048 320

6. OPERATE

Switching function: Manual zero calibration of CO

measuring system

Recommendation: Check the CO

concentration, especially during long-term tests.

If there is a large deviation (± 0.5 %) between the displayed actual value for CO

concentration and the nominal value, the measuring system can be recalibrated during operation.

Example: Display: 5.0 % CO

Comparative measurement: 4.2 % CO

Instruction

Select function level 3

Entry / Keys / Remarks

Hold down

Press

Display / Remarks / Status

Indicator lamp "control" flashes and the current function level is indicated on the flashing display

°C

Display zero point Release and press again

Enter determined CO

2 concentration

Press

°C

Start calibration process/exit function level

Release i

Press "CAL" is briefly displayed.

The corrected actual value is then displayed

50 048 320 29/45

7. START-UP

Switching function: Setpoint lock-in ON/OFF

To prevent unintentional or unauthorized modification of the setpoints for temperature, CO

, O

2 and rel. humidity, the setpoints can be "locked in".

The setpoints cannot be changed until the interlock has be cancelled again.

Factory setting: setpoints unlocked.

Instruction

Select function level 4

Display circuit status, factory setting: setpoints released

Change circuit status: locked setpoints

Change circuit status:

Release setpoints

Exit function level

Entry / Keys / Remarks

Hold down

Press

Display / Remarks / Status

Indicator lamp "control" flashes and current function level is indicated by flashing display

°C

Release and hold down again

Press

Release

Press any setpoint key

°C

Actual temperature, CO

, O

and rel. humidity values are displayed again after 1 minute

Actual temperature, CO

, O

and rel. humidity values are displayed again immediately

30/45 50 048 320

6. OPERATE

Switching function: Door heater ON/OFF

To adapt the unit to the ambient temperature in the place of installation, the door heater can be switched on or off (see page 13 for example).

Condensation can occur inside the unit, especially on the glass door or glass screen, when the door heater is switched off.

Factory setting: door heater switched on.

Instruction

Select function level 5

Display circuit status, factory setting:

Door heater ON

Change circuit status:

Door heater OFF

Change circuit status:

Door heater ON

Exit function level

Entry / Keys / Remarks

Hold down

Press

Display / Remarks / Status

Indicator lamp "control" flashes and the current function level is indicated by the flashing display

°C

Release and hold down again

Press

Release

Press any setpoint key

°C

Actual temperature, CO

, O

and rel. humidity values are displayed again after 1 minute

Actual temperature, CO

, O

and rel. humidity values are displayed again immediately

50 048 320 31/45

7. START-UP

The items specified in brackets refer to Fig. 4 on page 15.

Instruction

Open unit doors

Remove transit restraints and accessories from inside the unit

Carry out basic cleaning

Water reservoir

Entry / Keys / Remarks

Switch on unit

Adjust setpoints

Set temperature protection device

Select switching function(s) as required

Activate auto-start

Display / Remarks / Status

Fill unit with water (refer to page 16) -- observe max. level, use destilled or fully desalinated water

Mains switch (8) = " I "

Control and display panel (A)

All display elements come on for approx. 15 seconds

- auto-test of controller -

version:

The O

measuring system is started up automatically on completion of the auto-test.

Duration: approx. 5 min.

display reads "run".

Refer to page 17-20

Adjust Temperature Limit Controller

(6)

On control and display panel

(refer to page 18 and 26 to 30)

Refer to page 17

Press Indicator lamp "auto-start" flashes

Hold down for approx. 5 seconds

Close all unit doors Temperature displays "actual value"

2 displays "0.0",

displays "21.0" (optional) rel. humidity displays "actual value" auto-start routine runs automatically.

x Unit is set to entered temperature and relative humidity set

points.

x Relative humidity is built up.

x When a constant temperature and relative humidity are reached, the CO

2 and O

measuring system is calibrated automatically.

x Indicator lamp "auto-start" goes out.

x "auto-zero" routine for determining reference values is executed.

x Gas is admitted until the entered CO

/ O

setpoints are reached.

32/45 50 048 320

8. OPERATION

Operating control

The microbiological safety of the unit is largely dependent on the proper conduct of the persons using the unit. Refer to the Appendix "Basic rules of sound microbiological engineering practice".

Be sure to comply with the instructions contained in this manual as well as all applicable regulations and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598).

Before putting the unit into operation x x x

Put on all safety garments necessary to protect hands, face and body; remove jewelry.

Disinfect and clean the chamber and fittings regularly. Only use lint-free materials to wipe surfaces clean.

Do not use explosive disinfectants. When using alcoholic disinfectants, be sure they comply with all applicable regulations of your country (FRG: ZH 1/598).

During operation

Correct loading determines the temperature conditions in the chamber. If large objects or auxiliaries are placed inside the chamber, this can impair the temperature distribution in the unit.

When loading the unit, do not arrange articles too close to one another on the shelves

(use only approx. 70 % of surface area) in order to avoid impairing air circulation and ensure constant heat flow.

Do not impair temperature distribution. This is mainly caused by the following: x Covering the air vents.

x Voluminous objects or apparatus.

x Equipment releasing large amounts of heat.

Do not change the settings of the temperature protection devices as this will render load protection ineffective.

Operating interruptions

If operations are interrupted, make sure that no contamination hazards can occur.

x Remove, disinfect and clean any objects or auxiliaries put into the unit.

x Disinfect and clean the chamber.

x Disinfect and remove any residues.

x Disinfect the unit with hot air (disinfection routine).

50 048 320 33/45

9. SHUTDOWN

The unit may only be shut down by qualified staff trained to operate this equipment.

The unit must not present any risk of contamination after shutdown.

x Shut off the gas supply.

x Remove, disinfect and clean any objects or auxiliaries put into the unit.

x Disinfect and clean the chamber. Leave the unit doors open until the chamber is dry.

x Disinfect the unit with hot air (disinfection routine).

x Set the main switch to "0" and disconnect the power plug.

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10. MAINTENANCE

Cleaning / Disinfection

Be sure to disinfect the unit in compliance with the applicable regulations in your country

(FRG: ZH 1/598).

The chamber and fittings are decontaminated through hot air disinfection at 180°C.

The sensors can be left inside the unit during the disinfection process.

Do not spray the O

/ rel. humidity sensors (porous sintered material) with disinfectant.

Recommended disinfectant:

A surface disinfectant recommended by Thermo Electron Corporation can be ordered under following part numbers:

Spray bottle, 250 ml Part No.: 50052425

Refill bottle, 500 ml Part No.: 50051939

NOTE - Decription disingectant:

Details for efficiency and approvals are available on request.

Order Disinfection: x Remove samples, cultures, etc., from the unit.

x Switch off the unit.

x Remove shelves and carrier racks.

x Detach the rear panel of the inner vessel by lifting it up.

x Clean inner housing, shelves and carrier racks using a warm, skin-sensitive detergent

("Pril") and allow them to dry completely.

Do not wet sensors ( 4, 6, 8) with liquids and disinfectants.

8 7

1.) Lead-through and pressure compensation vent

2.) Steam outlet: Do not fill unit with any liquids -

only clean the surface

3.) Recirculated air blower

4.) O

sensor (optional)

5.) Pt 100 temperature sensors

6.) Relative humidity sensor.

7.) Tubular radiator for disinfection

8.) CO

measuring cell

Fig. 8: Rear wall of inner vessel x Also clean glass door(s) and seal(s) with detergent and allow them to dry completely.

x Then spray surfaces and seals with disinfectant and allow it to react in accordance with the manufacturer´s instructions for use.

x Then rinse surfaces with distilled water several times until all traces of disinfectant have been removed. Collect the washings.

x The surfaces of the unit can also be re-rinsed or sprayed with a 70% by vol. alcohol

solution (analytically pure isopropanol) if required.

50 048 320 35/45

10. MAINTENANCE x

Attention: Be sure to comply with the safety instructions and applicable regulations in your country!

Alcohols or solutions with an alcohol content of greater than 10% can ignite or form explosive gaseous mixtures when they come into contact with air and should therefore only be used in well- ventilated places. Do not expose to naked flame.

Equipment and component parts cleaned using alcohol or alcoholic solutions must not be exposed to naked flames or other possible hazard sources. Wait until dry before use! x Install the parts in the reverse order of removal as appropriate. x Disinfect the unit with hot air at 180°C (disinfection routine).

x After disinfection, put the unit back into operation as described and always check that it is safe to operate prior to loading.

Testing

The serviceability and safety of the unit are only guaranteed if the necessary testing, maintenance and repair work is carried out by the Heraeus service department or by staff authorized by us.

The unit should be checked for safety, leakproofing and serviceability at least once a year.

Equipment log book

We recommend you keep an equipment log book (refer to APPENDIX).

Keep a record of inspection and tests, calibration work and any major work carried out on the unit

(e.g. maintenance work, agents loaded, etc.) in this log book.

Replacement of electrical parts

Work on electrical components of the unit may only be carried out by Heraeus service department personnel and when the unit is in a deenergized state (disconnected from the mains supply).

Only use original replacement parts approved by Heraeus Instruments.

The sensors may only be replaced by authorized personnel of the operator.

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11. AUTHORIZED REPLACEMENT PARTS AND ACCESSORIES

The safety and serviceability of the equipment are only guaranteed if the approved original replacement parts specified below are used.

Use of other parts presents unknown risks and is not approved under any circumstances.

Replacement part

Instruction manual

Hose set for connecting unit to gas supply

Unit foot, upper part

Foot, height-adjustable, lower part

2 support brackets

Shelf, divided

Seal for inner casin front

Glass door for gas-tight glass screen, left

Glass door for gas-tight glass screen with feedthrough, left

Glass door for gas-tight glass screen, right

Seal for glass door on gas-tight glass screen

Filler canister

Equipment fuse

Sealing plug for feed-through (without hole)

Saeling plug for feed-through (with hole)

Subframe, 300 mm high

Subframe, 780 mm high

Pressure reducer for CO

Pressure reducer for N

Pressure reducer for O

Type

T 6,3 A (slow-blow)(2 pcs)

Order No.

50 042 724

50 062 701

50 044 921

50 029 587

50 011 380

50 069 921

50 048 705

50 030 020

50 030 021

50 030 022

50 041 536

50 029 126

03 002 641

03 669 008

50 029 827

50 031 348

50 029 597

03 429 937

03 429 942

03 429 943

50 048 320 37/45

12. TECHNICAL SPECIFICATIONS

TECHNICAL SPECIFICATIONS

UNITS: VALUE:

MECHANICAL:

Outer dimensions (W x H x D)

Inner dimensions (W x H x D)

Chamber volume

Divided Shelves (W x D)

Quantity included in scope of supply

Max. quantity

Max. surface load

Max. gross load of unit

Weight of unit excluding accessories

THERMAL:

Ambient temperature range

Temperature CO

-equipment control range CO

/ O

-equipment

Temperature deviation, temporal

(DIN 12 880, Part 2)

Temperature deviation, at: 37°C spatial (DIN 12 880, Part 2) 50°C mm mm l mm pcs pcs kg kg kg

°C

920 x 855 x 775

607 x 669 x 585 approx. 220

260 x 500

5 per shelf

107 (net)

+18 ... +30

Ambient temp.+5 ... +50

+7 ... +50

< + 0.1

< ± 0.5

< ± 0.7

Heating-up time with auto-start

(ambient temperature 22°C, unit empty) to: 37°C

Cooling time (ambient temperature 22°C unit empty)

from: 37 °C to 25°C

Heat release into surroundings at 37°C / 95% rel. humidity

Heat release into surroundings at 50°C / 95% rel. humidity

Heat release into surroundings during disinfection process

Recovery time at:

37°C / 5% CO

95% rel. humidity / 7% O

(closing the doors after they have been open for 30 s)

Temperature

Humidity

2 h h kWh/h kWh/h kWh/h min min min min approx. 5 approx. 5 approx. 0,16 approx. 0,22 approx. 0,59

< 2 (max. 1,4 % / min)

< 9

< 15

38/45 50 048 320

12. TECHNICAL SPECIFICATIONS

TECHNICAL SPECIFICATIONS

UNITS: VALUE:

Humidification

Water quality min. - max. level

Water demand to humidify: from 40 % rel. humidity to 95 % rel. humidity

Water consumption l ml ml/h

Distilled or fully desalinated

2.2

Total capacity

Measuring range and Setting range*

Control accuracy l

% r.H.

4.7

60 ... 95

± 1

GAS ENGINEERING

Carbon dioxide (CO

)

Oxygen (O

)

Nitrogen (N

)

Purity

Inlet pressure

Measuring range and Setting range

Control accuracy

Measuring range and Setting range

Control accuracy

ELECTRICAL:

Rated voltage

Rated frequency

Radio interference suppression (DIN VDE

0875)

Type of enclosure (DIN 40050)

Protection class

Overvoltage category (IEC 1010)

Degree of soiling

Rated current

Fuse protection:

Fuse

Circuit-break switch

Rated power consumption during incubation/disinfection

MISCELLANEOUS

Sound pressure level (DIN 45635 Part 1) max. r.H of the ambiance

Height

% bar

% CO

% O

A kW

2 dB (A)

% r. H m NN

99.5

0.8 up to max. 1

0 ... 20

± 0,1

3 ... 90

± 0,5

1/PE AC, 230 or 208

50/60

Interference suppression level N

IP 20

5,2

T 16 A slow-blow

G 16

1.2 / 1.2

< 50

2.000

* Under some circumstances, eg. when working with culture vessels without covers, the control range will be restricted to 90-95% since below this level evaporation of the medium will lead to an increase of the relative humidity in the chamber.

50 048 320 39/45

13. MATERIALS USED / DISPOSAL

Components

Outer housing

Inner vessel, shelves

Glass pane

Door seal of unit

Glass door seal

Thermal insulation

Control and display membrane

Heating systems

Leads

Other components

Material

Galvanized sheet steel, painted to RAL 9002

Stainless steel, material No. 1.4301

Sodium silicate glass

Magnetic core sheathed in soft PVC

Tempered silicon

PU foam sheet (CFC) incorporating non-woven needled glass fibre quilt (binder-free)

Polyethylene

Silicon-sheathed resistance heating conductor and tubular heater

Plastic-sheathed copper strands

Sheathed electrical components coated in various plastics partly mounted on printed circuit boards bonded with epoxy resin

Disposal

WEEE Compliance:

This product is required to comply with the European Union‘s Waste Electrical & Electronic Equipment

(WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Electron Corporation has contracted with one or more recycling/disposal companies in each EU

Member State, and this product should be disposed of or recycled through them. Further information on

Thermo Electron`s compliance with these Directives, the recyclers in your country, and information on

Thermo Electron products wich may assist the detection of substances subject to the RoHS Directive are available at www.thermo.com/WEEERoHS.

40/45 50 048 320

14. GAS CONSUMPTION

Gas consumption during operation of "cytoperm 2 gassed incubator"

Gas consumption corresponds to the gas quantity released at the back of the unit which has to be discharged safely into the outside air by ventilating the placement area.

Gasing with CO2 Gas consumation CO2

0,9

0,8

0,7

0,6

0,5

0,4

0,3

0,2

0,1

0 1 2 3 4 5 6

Time [min]

7 8 9 10

Amt of gas supplied: 8 l/h

Gas inlet pressure: 1 bar

Gasing with Oxygen (O2) / Nitroge (N2)

100

0 20 40 60 80

Amt. of gas supplied: O2 9,8 l/min

Time [min]

100

N2 7,4 l/min

Gas inlet pressure: 1 bar

120 140

0 2 4 6 8 10 12 14 16 18 20

CO2-concentration [% CO2]

Amt. of gas supplied: 8 l/h

Gas inlet pressure: 1 bar

Gas consumtion of N2/O2

0 10 20 30 40 50 60 70 80 90 100

O2-concentration [% O2]

Amt. of gas supplied: O2 9,8 l/min

N2 7,4 l/min

Gas inlet pressure: 1 bar

50 048 320 41/45

15. PH-VALUE OF CULTURE MEDIA

pH-value of culture media

Dependency on the CO2-concentration

6,8 7 7,2

pH-value

3,70

2,20

1,20

0,85

0,35

7,4

Media

DMEM

BME

MEM

Medium 199

Mc Coy

F 10

F 12

MEM

Media with

Hanks Salts

Na HCO

g/l

3,70

2,20

1,20

0,85

0,35

7,6

42/45 50 048 320

BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE *

General rules x Keep windows and doors in the working areas closed while work is in progress.

x Do not consume drinks, food or smoke in the working areas. Do not keep foodstuffs in the working areas.

x Lab coats or other protective garments must be worn in the working area.

x Oral pipetting is prohibited. Use pipetting aids.

x Only use needles and syringes if absolutely necessary.

x With all manipulators, be sure to avoid aerosol formation as far as possible.

x On completion of work and before leaving the working area, clean hands thoroughly. Disinfect and remoisturize hands if necessary.

x Keep working areas clean and tidy. Only equipment and materials actually required should be on the worktops. Keep stocks in the areas or cabinets provided for them only.

x Regularly check the identity of the biological agents used if this is a requirement for hazard potential assessment. The length of the interval between checks should be set according to hazard potential.

x With regard to the handling of biological agents, employees must be issued with verbal instructions relating to the workplace before commencing work. These instructions must be re-issued at least once annually thereafter.

x Employees inexperienced in the fields of microbiology, virology or cellular biology must be given thorough instructions and be supervised.

x Vermin and pests must be combatted regularly if necessary.

Handling of pathogenes

Also heed the following basic rules regarding the handling of pathogenes: x Disinfect all workplaces on a daily basis. It may be necessary to switch disinfectants as a precaution against resistant germs.

x Do not wear protective garments outside the working areas.

x Contaminated equipment must be autoclaved or disinfected prior to cleaning.

x Waste containing pathogenes must be collected safely and rendered harmless through autoclaving or disinfection.

x If infectious substances are spilt, the contaminated area must be closed off immediately and disinfected.

x If work is conducted using human pathogenes against which an effective antidote is available, all employees who are not yet immune must be vaccinated and checked regularly for immunity in the appropriate manner.

x The health of employees must be monitored by medical check-ups, i.e. employees must undergo an initial check-up before commencing work and subsequent annual check-ups. Medical check-ups should be conducted in accordance with the applicable employers´ liability insurance association guidelines, in particular G 24, "Skin diseases", and G 42, "Infectious diseases". As generally recognized rules of industrial medicine, they enable the doctor to assess, evaluate and record the results of examinations according to the same criteria.

* Apply to cell cultures as appropriate

Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employer´s liability insurance association for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg

50 048 320 43/45

BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE * x Potentially hazardous, genetically engineered organisms, viruses and subviral agents must be handled in accordance with employers´ liability insurance association guideline G 43 entitled "Biotechnology".

x Instructions for administering first aid in the event of accidents involving pathogenic microorganisms and viruses must be immediately to hand in the working area. The person in charge must be notified of all accidents immediately.

Depending on hazard potential, it may be necessary to take further safety precautions: x Use of class I, class II (type-approved)** or class III safety workbenches (air flow pointing away from experimenter).

x Restricting and supervising access to specific areas.

x Use of special protective garments and breathing apparatus.

x Disinfection of all materials containing pathogenes before they leave the worktop.

x Maintaining a partial vacuum in the working area.

x Reducing the germ count in the exhaust air by taking appropriate measures, such as using highperformance suspended-matter filters.

Handling of human- and animal-pathogenic biological agents

The following general rules also apply to the handling of human- and animal-pathogenic biological agents: x Human-pathogenic biological agents may only be handled with approval under the terms of the Federal

Epidemics Act.

x Approval under the terms of the Federal Animal Epidemics Act and the Ordinance concerning Agents of

Animal Epidemics is required to handle agents of animal epidemics.

x Pregnant women or nursing mothers are not permitted to handle infectious and human-pathogenic biological agents or materials containing these agents.

* Apply to cell cultures as appropriate

**Manufacturers´ certificates can be found in the memoranda of the Berufsgenossenschaft Chemie (employer´s liability insurance association for chemicals) entitled "Sichere Chemiearbeit" (Safe chemistry) and the Berufsgenossenschaft für

Gesundheitsdienst and Wohlfahrtspflege (employer´s liability insurance association for health service and welfare).

They can also be obtained on request from the test centre of the expert committee on "Health care and welfare"

(Berufsgenossenschaft für Gesundheitsdienst und Wohlfahrtspflege, Prüfstelle des Fachauschusses "Gesundheitsdienst und Wohlfahrtspflege, Pappelallee

35-37, 22089 Hamburg, Germany).

44/45 50 048 320

Betriebsanleitung Sicherheitswerkbank HERAsafe KS / KSP

Internet: http://www.thermo.com

71

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Key features

Precise temperature control
CO2 concentration regulation
Optional O2 control
Relative humidity control
Hot-air disinfection
Auto-start and auto-zero calibration
Error code display

Frequently asked questions

The temperature range can be set between +7°C and +50°C, but it must be at least +5°C above the ambient temperature.

The incubator uses hot-air disinfection at 180°C for approximately 3 hours. The entire process takes around 13 hours.

Only use fully desalinated or distilled water that has been sterilized or sterile water that has been demineralised. Distilled or autoclaved water is suitable.