PHCbi MCO-18ACL-PA MCO CO2 Incubator Operating Instructions

Operating Instructions

CO

2

Incubator

MCO-18ACL

This product is for research use only.

Not for clinical diagnosis or treatment of humans or animals.

Please read the operating instructions carefully before using this product, and keep the operating instructions for future use.

See page 43 for model number.

CONTENTS

INTRODUCTION ................................................................................................. 2

PRECAUTIONS FOR SAFE OPERATION .......................................................... 3

LABELS ON INCUBATORS ................................................................................ 8

ENVIRONMENTAL CONDITIONS ...................................................................... 8

INCUBATOR COMPONENTS ............................................................................. 9

Control panel and keypad .............................................................................. 11

Remote alarm terminal ................................................................................... 12

INSTALLATION SITE ........................................................................................ 13

INSTALLATION ................................................................................................. 14

Connecting CO

2

gas cylinder ......................................................................... 15

PREVENTING CONTAMINATION .................................................................... 16

PRECAUTIONS FOR CULTURES .................................................................... 17

INITIAL CLEANING METHOD ........................................................................... 18

REMOVING INNER ATTACHMENTS ............................................................... 19

INSTALLING INNER ATTACHMENTS .............................................................. 21

FILLING HUMIDIFYING PAN ............................................................................ 22

WATER LEVEL SENSOR (MCO-18ACL with MCO-18UVS3) .......................... 24

ROUTINE MAINTENANCE................................................................................ 25

CORRECT OPERATION ................................................................................... 25

SETTING CHAMBER TEMPERATURE AND CO

2

DENSITY ........................... 26

SETTING OF ALARM RESUME TIME .............................................................. 27

Operation after power failure .......................................................................... 27

KEY LOCK FUNCTION ..................................................................................... 28

UV LAMP (MCO-18ACL with MCO-18UVS3) ......................................................... 29

Precautions when using UV lamp .................................................................. 30

Setting UV lamp ON period ............................................................................ 31

Lighting UV lamp for 24 hours ........................................................................ 32

ALARMS, SAFETY, AND SELF-DIAGNOSIS ................................................... 33

CALIBRATION

Temperature calibration ................................................................................. 34

CO

2

calibration ............................................................................................... 35

TROUBLESHOOTING ....................................................................................... 36

DISPOSAL OF UNIT .......................................................................................... 37

AUTOMATIC CO

2

CYLINDER CHANGEOVER ................................................ 38

STACKING INCUBATORS ................................................................................ 40

SPECIFICATIONS ............................................................................................. 42

PERFORMANCE ............................................................................................... 43

SAFETY CHECK SHEET .................................................................................. 44

1

INTRODUCTION

■ Read the operating instructions carefully before using the Product and follow the instructions for safety operation.

■ PHC Corporation disavows any responsibility for safety if the Product is used for other than the intended use or used with any procedures other than those given in the operating instructions.

■ Keep the operating instructions in a suitable place so that it can be referred to as necessary.

■ The contents of the operating instructions are subject to change without notice for improvement of performance or functions.

■ Contact our sales representative or agent if any page of the operating instructions is lost or the page order is incorrect.

■ Contact our sales representative or agent if any point in the operating instructions is unclear or if there are any inaccuracies.

■ No part of the operating instructions may be reproduced in any form without the expressed written permission of PHC Corporation.

IMPORTANT NOTICE

PHC Corporation guarantees this product under certain warranty conditions. However, please note that

PHC Corporation shall not be responsible for any loss or damage to the contents of the product.

<Intended Use>

This equipment is designed for cell and tissue culture for laboratory use. Not for clinical diagnosis or treatment of humans or animals.

2

PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with the operating instructions as it contains important safety advice.

Items and procedures are described so that you can use this unit correctly and safely.

If the precautions advised are followed, this will prevent possible injury to the user and any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property.

Symbol shows;

This symbol means caution.

This symbol means an action is prohibited.

This symbol means an instruction must be followed.

Be sure to keep the operating instructions in a place accessible to users of this unit.

WARNING

As with any equipment that uses CO

2

gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices.

3


USA Only (Model with a lamp): This product has a lamp that contains mercury. Disposal may be regulated in your community due to environmental considerations. For disposal or information, please visit PHC website: https://www.phchd.com.

Contains mercury / Contenu avec mercure

For more information on safe handling Pour plus de renseignements sur les procedures, the measures to be taken in procédures de manutention sécuritaire, les case of accidental breakage and safe disposal options visit: ec.gc.ca/mercure-mercury/.

Dispose of or recycle in accordance with applicable laws. mesures à prendre en cas de bris accidentel et les options d’élimination sécuritaire visitez: ec.gc.ca/mercure-mercury/.

Mettez au rebut ou recyclez conformément aux lois applicables.

4

WARNING

Do not use the unit outdoors.

Current leakage or electric shock may result if the unit is exposed to rain water.

Only qualified engineers or service personnel should install the unit.

The installation by unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from turning over.

If the floor is not strong enough or the installation site is not adequate, this may result in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location.

This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod.

Such grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles.

Improper use may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet hand.

This may cause electric shock.

5


WARNING

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when the liquid is spilled.

Never bind, process, or step on the power supply cord, or never damage or break the power supply plug.

A broken power supply cord or plug may cause fire or electric shock.

Do not use the power supply cord if its power supply plug is loose. Such power supply cord may cause fire or electric shock.

Never disassemble, repair, or modify the unit yourself.

Any such work carried out by an unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit.

Continued abnormal operation may cause electric shock or fire.

When removing the power supply plug from the power supply outlet, grip the power supply plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the power supply plug before moving the unit. Take care not to damage the power supply cord. A damaged cord may cause electric shock or fire.

Disconnect the power supply plug when the unit is not used for long periods.

Keeping the connection may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children do not have access and that doors cannot be closed completely.

The disposal of the unit should be accomplished by appropriate personnel.

Remove doors to prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

Do not position this unit and the other unit so that it is difficult to operate the disconnection of the power supply plug.

Failure to disconnect the power supply plug may cause fire if there is something wrong with the unit.

6

CAUTION

This unit must be plugged into a dedicated circuit protected by branch circuit breaker.

Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may cause fire resulting from abnormal heating.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power switch.

The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet when you request any repair or maintenance for the safety of service personnel.

7

LABELS ON INCUBATORS

Warning and caution labels are attached to the incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to prevent electric shock. Only a qualified engineer or service personnel should be allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Temperature 5 o C to 40 o C

■ Maximum relative humidity 80 % for temperature up to 31 o C decreasing linearly to 50 % relative humidity at 40 o C;

■ Mains supply voltage fluctuations up to ±10 % of the nominal voltage;

■ Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;

■ Temporary OVERVOLTAGES occurring on the mains supply;

■ Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);

8

INCUBATOR COMPONENTS

1

15

Handles

Handles

5

Rear Right Side

4

13

10, 11

7 8

When an optional UV system set

MCO-18UVS3 is installed.

21

14

9 (inside)

17

3

16

6

2

19,20 (inside)

When an optional UV system set

MCO-18UVS3 is installed.

18

12

Rear Left Side

9


1.

Outer door: Sticks to frame with magnetic seal. Door heater is installed in the outer door panel.

The door opening is reversible. Contact our sales representative or agent to change the door hinge from left to right or vice versa.

2. Inner door: Made of tempered glass, however avoid excessive impact on the glass.

3. Leveling feet: Screw type for adjusting the height. Adjust the feet so that the incubator can be level.

4. Tray: Can be pulled toward you.

5. Tray support: Can be removed by lifting the front side and pulling toward you.

6. Side support: Right and left side supports can be removed for disinfection. See page 19 and 20.

7. Fan cover: Inlet of circulating air. Removable.

8. Duct: Flow path for circulating air. Removable.

9. Fan (inside the rear duct): Made from polypropylene resin. Can be disinfected by an autoclave.

10. Sample air outlet: This also functions as an internal gas outlet. Normally, cover this outlet with the sample air outlet cap.

11. Sample air outlet cap: Always attach this cap except at the time of using of sample air outlet.

Note: Operation without sample air outlet cap may result low humidity or condensation on the outer door.

12. Connecting port A/B for CO

2

gas pipe: When an optional component MCO-21GC (gas auto changer) is installed, both A and B are available. If MCO-21GC is not used, only A is available. Refer to page 15 for gas cylinder connection. Ensure that the gas pressure is set at 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.4 psi(G)). Refer to page 42 for gas auto changer.

13. Door switch: Detects the door opening/closing and stops the circulating fan, the UV lamp* and electromagnetic valve for CO

2

when the outer door is open.

14. Humidifying pan: Use sterile distilled water to fill the humidifying pan. (When an optional UV system set MCO-18UVS3 is installed) Install the humidifying pan properly so that it can be covered with the humidifying pan cover.

15. Remote alarm terminal: Refer to page 12.

16. Access port: When not in use, cap with the silicon caps on both outside and inside.

17. Power switch: Main switch of the incubator (ON-“I”, OFF-“O”). Also functions as an over-current breaker. The switch is covered by a switch cover to prevent the accidental push. To turn on or off the switch, remove the switch cover by loosening the screw.

18. Humidifying pan cover*: Prevents UV light being exposed to the chamber. When filling the humidifying pan, lift the front side and take out the humidifying pan. See page 22 for details.

19. UV lamp*: This UV lamp does not generate ozone. Never look at the UV light directly. For replacement, contact our sales representative or agent.

20. Water level sensor*: Detects the water level in the humidifying pan. See page 24 for details.

21. Glow starter*: The glow is started when the UV lamp light.

*When an optional UV system set MCO-18UVS3 is installed.

10

Control panel and keypad

7

1

2

3

4

5

6

8

9

10

11

12

13

14

17 16 15

1. Digital temperature indicator (TEMPERATURE o C): Normally, this indicator shows the chamber temperature. In the setting mode, it shows the set value of the chamber temperature. If the self diagnostic function detects any abnormality, an error code will be displayed.

2. Heater lamp (HEAT): This lamp lights when the heater is energized.

3. UV indicator (UV): This lamp lights when the UV lamp is ON. The blink of this indicator recommends the replacement of the UV lamp. See page 29 for the details. When an optional UV system set MCO-18UVS3 is installed.

4. Door lamp (DOOR): This lamp lights when the outer door is open.

5. Water level alarm lamp (RH PAN): This lamp blinks when the water in the humidifying pan is less than approximately 0.8 L. When an optional UV system set MCO-18UVS3 is installed.

6. High limit regulator : This regulator is used to set the high limit temperature. See page 26 for the details.

7. Over heat lamp (OVER HEAT): This lamp lights when the chamber temperature reaches the high limit temperature.

8. CO

2

inject lamp (INJECT): This lamp lights when CO

2

gas is being injected.

9. Digital CO

2

density indicator (CO

2

%): Normally, this indicator shows the CO

2

density in the chamber. In the setting mode, it indicates the set value of the CO

2

density. In the calibration mode, the decimal point in the digital CO

2

density indicator blinks.

10. Set key (SET): Pressing this key to enter the setting mode, and the digits to be set will blink.

11. Calibration key (CAL): Pressing this key to enter the CO

2

calibration pre-conditioning mode.

Normally when the outer door is opened, the fan motor will stop operating. This mode allows the fan motor to continue to circulate the chamber environment for 5 minutes to ensure accurate CO

2

uniformity inside the chamber. At this time, by pressing this key for approximately 5 seconds, the incubator enters the temperature calibration input mode. And then pressing this key again, the incubator enters the CO

2 calibration input mode. See page 34 and 35 for the details. Also, used to change the UV lamp ON period. See page 31 for the details.

11


12. Alarm buzzer stop key (BUZZER): Press this key to silence the buzzer when the alarm operates and the buzzer sounds (The remote alarm is not stopped).

13. CO

2

gas supply line indicator (A/B): The lamp for the supply line currently in use lights up provided that MCO-21GC gas auto changer (option) is installed.

14. CO

2

gas supply line switching key : This key to select CO

2

gas supply line is available only when a gas auto changer MCO-21GC (option) is installed. When one CO

2 cylinder is empty, the CO

2 is supplied by the other cylinder automatically.

15. Enter key (ENT): Pressing this key memorizes the set value in the controller.

16. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value to shift. In key lock mode, pressing this key makes key lock ON or OFF.

See page 28 for details.

17. Digit shift key ( ): Pressing this key in the setting mode causes the changeable digit to shift.

Pressing this key more than 5 seconds enters key lock mode. See page 28 for the key lock.

Remote alarm terminal

The terminal of the remote alarm is installed at the rear right side of the incubator. The alarm is outputted from this terminal. Contact capacity is DC 30 V, 2 A.

Contact output: between COM. and N.O. between COM. and N.C.

12

Note:

The alarm is actuated when the power supply cord is disconnected from the outlet or the power switch is

OFF.

• The remote alarm cannot be stopped by pressing the alarm buzzer stop key (BUZZER) since the remote alarm is not conjunct with the alarm buzzer stop key (BUZZER).

Remote alarm terminal

INSTALLATION SITE

For correct operation of the incubator, install it in a location with the following conditions.

WARNING

When using CO

2

gas for control, make sure that there is adequate ventilation . Using CO

2

gas in a small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when opening the incubator doors, do not directly inhale the air in the chamber.

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO

2

de l’air peut s’élever et peut

être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la porte.

 Normal air environment

Install the incubator in an environment with normal air.

 Do not expose to direct sunlight

Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is operated in direct sunlight, performance will be adversely affected.

 Separate from heat sources

Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.

Heat will adversely affect the performance of the incubator.

 Ambient temperature at least 5  C lower than set temperature

The control temperature of the incubator is at least 5  C higher than the ambient temperature. For example, if the chamber is controlled at 37  C, the ambient temperature normally must be no more than

32  C. Do not allow the ambient temperature to become too high.

 Strong and level floor

Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that the installation is stable. An unstable surface may result in vibration or noise.

WARNING

Install the incubator at a location that can support the weight.

If the floor is not strong enough or if the installation is insufficient, the incubator may fall over and cause injury.

Always make sure that the floor is strong, even, and level, and that the incubator will not tip over .

An insufficient installation may result in injury due to water leakage or the incubator falling over.

 Low humidity

Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result in current leakage or electric shock.

WARNING

Do not use the incubator outdoors.

If the incubator is exposed to rain water, it may result in current leakage or electric shock.

Never install the incubator in a moist location, such as near a sink or water line, or where it is likely to be exposed to water.

In addition, do not install it near water or steam pipes . Moisture can cause the insulation to deteriorate, which may result in current leakage or electric shock.

 No inflammable or corrosive gas

Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing, resulting in current leakage or electric shock.

 No falling objects

Do not install the incubator in a location where there is the possibility of objects falling from above. Doing so may result in damage or accident.

13

INSTALLATION

1. Remove the packing tape and clean up.

Remove all the tapes that are securing the doors and inner attachments. Open the doors for ventilation. If the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an undiluted solution may damage the plastic. Follow the diluting instructions for the detergent that is used.)

Wipe off the residual detergent with a wet cloth and then wipe off any moisture.

Note:

Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion of the cord coating.

WARNING

Do not leave plastic wrapping bags within reach of children.

If a bag is placed over a child’s head, it can block mouth and nose and cause suffocation.

2. Adjust the leveling feet.

Adjust the leveling feet by turning them counterclockwise to level the incubator. (See the illustration on the right.)

3. Ground the incubator.

Ground the incubator during installation to prevent electric shock in case the insulation is not sufficient. If there is no ground wire at the location, contact with qualified service personnel.

 When a ground must be installed

If a grounded 3-pole outlet is not available, then a ground must be installed. Contact with qualified service personnel.

Leveling feet

WARNING

To prevent electric shock, always ground the incubator.

If grounding is not possible, then have a ground installed by qualified service personnel. If the incubator is not grounded, it may result in electric shock.

Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.

Doing so may cause electric shock .

 Installing a ground fault circuit breaker

If using the incubator in a location with moisture or humidity cannot be avoided, then it is recommended that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the incubator). Have the circuit breaker installed by qualified service personnel.

CAUTION

Do not climb on the incubator or place objects on top of it.

Doing so may damage it or cause it to fall over, resulting in injury. If it is to be stacked, refer to page 40 and stack it securely.

 When the incubator is not in use

Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the chamber is completely dry before closing the doors. Failure to do so may result in damage.

 Before moving the incubator

Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug from the outlet, and make sure that the power supply cord will not be damaged. Failure to do so may result in electric shock or fire.

14

Connecting CO

2

gas cylinder

WARNING

When connecting a gas cylinder to the incubator, confirm the gas type . Confirm that the connections are secure and that no gas will escape . Be sure to use the specified pressure . Using an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping gas.

Install the incubator in a location with adequate ventilation.

If adequate ventilation cannot be provided, then install an alarm system using CO

2

and O

2 densitometers.

1. Use a liquefied CO

2

gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.

2. Install a gas regulator rated at 25 MPa(G) (250 kgf/cm 2 (G), 3600 psi(G)) for the primary pressure, and

0.25 MPa(G) (2.5 kgf/cm 2 (G), 36 psi(G)) for the secondary pressure on the CO

2

gas cylinder.

3. Using the gas tube that is provided, connect the gas regulator to the connecting port A/B for CO

2

gas pipe (located at the lower left-hand side on the rear panel of the incubator).

Note:

If CO

2

is supplied to multiple CO

2

incubators from a single gas cylinder, a CO

2

solid will be formed in the gas regulator. The gas regulator safety valve will operate, and there may an explosive sound.

When the MCO-21GC is not mounted

Using the gas tube that is provided, connect the gas regulator for the CO

2

gas cylinder to connecting port

A for CO

2

gas pipe on the incubator. After connecting the gas tube, make sure that no gas is leaking.

When the MCO-21GC is mounted

Connect a pair of CO

2

gas cylinders with gas regulators independently. The gas supply line will be switched automatically. Connect the main cylinder to connecting port A for CO

2

gas pipe and the reserve cylinder to connecting port B for CO

2

gas pipe. After connecting the cylinders, make sure that no gas is leaking.

 For details on installing the optional gas auto changer (MCO-21GC), refer to the MCO-21GC installation guide. For details on using the MCO-21GC, refer to page 38.

4. Set the CO

2

gas on the secondary side to 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.4 psi(G)) for gas injection. As the pressure increases, the CO

2

gas density control range will increase. Excessive pressure may cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen deprivation due to the escaping of gas. If gas lines come loose, the incubator must be repaired.

 The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities are found during inspection, replace the lines immediately.

15

PREVENTING CONTAMINATION

To prevent contamination of the chamber, select a suitable installation site.

 Avoid locations with high temperatures or humidity.

Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in the air.

 Avoid locations with passers-by or drafts.

Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry of microorganisms into the chamber.

 If possible, use a cleanroom.

To achieve a better culture result, it is recommended that a cleanroom be used if one is available.

 Use clean containers.

The greatest cause of contamination is dirty containers used in cultures. Be careful not to get containers or trays dirty when taking them in and out.

 Keep the chamber clean.

Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details, refer to “ROUTINE MAINTENANCE “on page 25.

 Use sterile distilled water in the humidifying pan.

Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because red rust-like suspended particles might be generated.

The water level alarm lamp (RH PAN) on the control panel blinks when the water level is low. Quickly refill the water in the humidifying pan when the water level alarm is displayed. Adding low-temperature water will significantly lower the temperature in the chamber. Clean the humidifying pan once a month.

 Keep the incubator out of direct airflows from air conditioners or fans.

Cool airflow from an air conditioner may cause condensation and lead to possible contamination.

16

PRECAUTIONS FOR CULTURES

 Leave space between culture containers.

Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate spacing may result in uneven temperature distribution and CO

2

gas density.

 Do not place harmful materials in the chamber.

Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause damage resulting from discoloration or corrosion.

 Close the inner door.

Always close the inner door before closing the outer door. Failure to close the inner door will adversely affect performance even if the outer door is closed.

 Open and close the doors gently.

Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the glass to confirm that the UV lamp is OFF.

 Be careful when closing the outer door.

Use the handle when closing the outer door. Holding the door in other places may cause injury by getting fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door coming loose or the incubator falling over, or it may cause current leakage or electric shock.

 Be careful of the inside of the outer door.

The inside of the outer door may become hot.

 Avoid using excessive force on the inner door.

Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result in injury from breaking the glass.

 Check the cause of any alarm buzzer.

If an alarm buzzer sounds while the incubator is in use, immediately check the cause of the alarm. For details on what may cause an alarm buzzer to sound, refer to page 33.

● Thermal conductivity CO

2

sensor

It is not abnormal that the thermal conductivity CO

2 sensor displays CO

2

density higher than the actual density when the chamber humidity temporarily goes down. And take care of the level of humidifying water because the lack of water affects the CO

2

density.

17

INITIAL CLEANING METHOD

Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the chamber and the inner attachments thoroughly. To keep the chamber clean is essential to get the proper performance out of the incubator. Use the following steps to clean the incubator properly.

1. Remove the inner attachments, referring to “REMOVING INNER ATTACHMENTS ” on page 19.

2. Clean the removed inner attachments, the chamber inside walls and the inner door gaskets with a cloth or sponge soaked in neutral detergent, diluted by 5 % or less. (Undiluted detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.) (Fig. 1)

CAUTION

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause discoloration, corrosion, or rusting.

Be careful to keep the diluted detergent or water out of the temperature sensor, the CO

2

gas injection hole and the fan motor shaft bearing (Fig.2 ). Also, do not wash the temperature sensor, the water level sensor and the UV lamp using detergent. Doing so may cause failure. (Fig.2)

3. Soak a gauze or unwoven cloth in distilled water and wring it tightly, and then wipe off the residual detergent thoroughly.

4. Wash the silicon caps (2 pcs) for the access port and the fan using the above mentioned detergent and rinse them with distilled water, and then autoclave them for sterilization (121 o C, 20 minutes).

5. Wipe up the inside walls, and the inner attachments like trays thoroughly with a cloth or unwoven cloth soaked in alcohol for disinfection. Be careful not to leave any residue alcohol.

6.

Reinstall the inner attachments correctly and securely, referring to “INSTALLING INNER ATTACHMENTS ” on page 21.

Fig. 1

Silicon cap

Temp. sensor

CO

2

sensor

(UV lamp)

(Water level sensor)

Fig. 2

18

REMOVING INNER ATTACHMENTS

CAUTION

Wear rubber gloves when performing maintenance on the chamber.

Failure to wear gloves may result in cuts or abrasions from sharp edges or corners.

Be careful not to damage the water level sensor or the UV lamp in the duct (if an optional UV system set

MCO-18UVS3 is installed.).

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause discoloration, corrosion, or rusting.

1.

Turn OFF the power switch of the incubator.

2. Open the outer and inner doors and pull out all the trays. (Fig. 1)

3. Lift the front of the tray supports and pull them out. (Fig. 2)

4.

(When an optional UV system set MCO-18UVS3 is installed) Lift the humidifying pan cover off from the pins at the rear side. (Fig. 3)

5.

Pull out the humidifying pan. (Fig. 4)

6.

Loosen the two screws securing the fan cover and take off the fan cover. (Fig. 5)

7.

Lift the duct and remove it from the pins at the rear side. (Fig. 6)

Pins

Tray support

Fig. 1 Fig. 2

Humidifying pan cover

Fig. 3

Duct

Humidifying pan

Fig. 4

Screws

Fan cover

Fig. 5 Fig. 6

19

REMOVING INNER ATTACHMENTS

8.

Remove the fan by pulling out the central spring and then by pulling out the fan. (Fig. 7)

9.

Remove the screw securing the clamp for the side support. (Fig. 8)

10.

Remove the clamp. (Fig. 9)

11.

Lift the side supports off of the pins. (Fig. 10)

12.

Remove the silicon caps of the access port, one each from interior (Fig. 11) and exterior (Fig. 12).

1. Pull out the central spring

Screw

Clamp

Side support

2. Pull out the fan

Fig. 7

Silicon cap

Fig. 8

Silicon cap

Fig. 9

Fig. 11 Fig. 12

20

Fig. 10

INSTALLING INNER ATTACHMENTS

Use the following steps to install the inner attachments properly.

1. To reinstall all the attachments, perform the procedure in reverse order from step 12 on page 20.

2. When installing the fan, insert it to the motor shaft securely. Lightly turn the fan by manual to make sure that it does not touch the rear panel. Then install the central spring. (Fig.1)

CAUTION

If the fan is not inserted deep enough, the intended velocity performance cannot be achieved, which may cause culture failure or insufficient decontamination.

3. To install the duct, confirm 4 pins are securely installed in the 4 holes of duct. (Fig. 2)

CAUTION

If the duct is fixed insecurely, the intended velocity performance cannot be achieved, which may cause culture failure or insufficient decontamination.

1. Push the fan deeply.

2. Install the central spring afterwards.

3. Lightly turn the fan manually to make sure that it does not touch the rear panel.

Fig.1 Fig.2

4. To install the side support, confirm the direction of fixing holes as shown in Fig. 3.

5. As shown in Fig. 4, set the tray with only the front edge bent down. If the tray is set in the wrong direction, it may not be level and may become unstable.

Fig. 3

Bent down

Fig. 4

21

FILLING HUMIDIFYING PAN

Use the following procedure to fill the humidifying pan or to replace the water.

1. (When an optional UV system set MCO-18UVS3 is installed) Lift the humidifying pan cover. (Fig. 1)

2. Pull out the humidifying pan. (Fig. 2)

3. Dispose of the remaining water in the humidifying pan and clean the humidifying pan with a diluted detergent. Then rinse it thoroughly with distilled water and wipe it with alcohol for disinfection.

4. Wipe all moisture from the bottom of the chamber.

5. Return the humidifying pan to the chamber and pour sterile distilled water (approx. 1.5 L, preheated to 37  C). (Fig. 3)

Note:

• Preheat the water to 37  C to be poured into the humidifying pan. Adding low-temperature water will lower the temperature and humidity in the chamber.

Fig. 1

Fig. 2

CAUTION

Set the humidifying pan lengthwise at the center of chamber width. At that time, push the humidifying pan all the way until short side of the pan contacts chamber rear surface. Improper installation may cause condensations.

6. Set the humidifying pan with the inner side flush against the back, and close the inner door and the outer door.

Fig. 3

(When an optional UV system set MCO-18UVS3 is installed)

Set the humidifying pan with the inner side flush against the back, and replace the humidifying pan cover

(Refer to page 24). Close the inner door and the outer door, and confirm that water level alarm lamp (RH

PAN) on the control panel is OFF.

Note:


If the water level alarm lamp (RH PAN) blinks, use the above procedure to replenish the water.

22

CAUTION


When refilling the water in the humidifying pan, always wipe off any dirt from the water level sensor with alcohol for disinfection . While doing that, be careful not to apply excessive force to the sensor lead wire.

Note:

The humidity in the incubator chamber is adjusted to the optimum setting. To prevent condensation on the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the humidifying pan at the bottom of the chamber (on the inside of the humidifying pan cover), but this does not indicate a problem.

23

WATER LEVEL SENSOR

(MCO-18ACL with MCO-18UVS3)

The water level sensor is used with the MCO-18ACL with an optional UV system set MCO-18UVS3 installed. The water level sensor is set automatically when the humidifying pan is installed. Take care not to damage the water level sensor when removing or installing the humidifying pan.

When the humidifying pan is removed (side view)

Water level sensor

Humidifying pan

When the humidifying pan is installed (side view)

Note: Make sure that the humidifying pan is pushed all the way to the back and that the water level sensor comes down when you install the humidifying pan.

Note:

• Lift the water level sensor before installing the humidifying pan if the water level sensor is in the lower position after maintenance.

• When installing the humidifying pan, make sure that the humidifying pan is set properly and that water level sensor comes down into the humidifying pan. The water level alarm lamp (RH PAN) blinks if the water level sensor does not come down completely. If necessary, set the humidifying pan again in the proper location.

• The water level sensor detects the water level every 30 minutes and just after the outer door is closed. It takes several seconds to detect the water level. Therefore, the water level alarm lamp may flash a few times after the outer door is closed even when the humidifying pan is full.

CAUTION

Foreign particles on the water surface can adhere to the water level sensor and fittings by capillary action because the water level sensor is always in the water. The adhered foreign particles degrade the water level sensor performance and the water level alarm lamp may blink even though there is sufficient water in the humidifying pan. Be sure to wipe OFF any dirt on the water level sensor with alcohol for disinfection whenever you change the humidifying water. When cleaning the water level sensor, take care not to apply excessive force to the lead wires.

24

ROUTINE MAINTENANCE

To use this unit in a clean condition, clean the chamber and all the inner attachments at least once a month.

1. Remove all the inner attachments by the procedures shown on page 19.

2. Clean the chamber and all the inner attachments by the procedures shown on page 18.

3. Install all the inner attachments by the procedures shown on page 21.

● When there is excessive dirt, contact our sales representative or agent.

CORRECT OPERATION

Use the following procedure to start trial operation or actual operation of the incubator.

1. Install the incubator correctly, referring to “INSTALLATION ” on page 15.

2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the chamber and all the inner attachments, referring to above “ROUTINE MAINTENANCE”.

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 22.)

4. Connect the power supply cord to the outlet. Turn ON the power switch on the rear left side of the incubator.

Note:

The humidity in the incubator chamber is adjusted to the optimum setting. To prevent condensation on the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the humidifying pan at the bottom of the chamber (on the inside of the humidifying pan cover), but this does not indicate a problem.

25

SETTING CHAMBER TEMPERATURE AND CO

2

DENSITY

Table below shows the basic procedure for setting the chamber temperature and CO

2

density. The high limit temperature alarm setting is also shown in the table. Perform key operations in the sequence indicated in the table. The example in the table is based on the assumption that the desired temperature is 33 o C and CO

2

density is 5 %. Adjustment of the high limit regulator should be executed after the chamber temperature reaches the stable condition.

Note:

• The incubator is set at the factory so that the chamber temperature is 37 o C and CO

2

density is 0 %.

• When starting the incubator for the first time or after not using it for an extended period of time, allow at least 8 hours for the chamber temperature, humidity, and CO

2

sensor to become stable after setting the desired chamber temperature at 0 % CO

2

density. Then change the setting to the desired CO

2

density.

Basic operation sequence (Example: Chamber temperature: 33 o C, CO

2

density: 5 %)

Description of operation Key operated

1 Turn the power switch ON. ----

Indication after operation

The current chamber temperature is displayed in the digital temperature indicator.

2 Press set key. SET The left digit blinks.

3

By pressing numerical value shift key and digit shift key, set the figure to 33.0.

4 Press enter key. ENT

When pressed, the changeable digit is shifted.

When pressed, the figure of settable digit changes.

Set temperature is memorized.

Left digit in the digital

CO

2 density indicator blinks.



5

By pressing digit shift key and numerical value shift key, set the figure to 05.0.

6 Press enter key.

(Executed after the chamber temperature reaches the stable condition)

7

Adjust high limit regulator so that the alarm temp. is at least 1 o C higher than set temperature.

ENT

----

Set CO

2

density is memorized.

In the digital CO

2 density indicator, HI is displayed.

In the digital temperature indicator, high limit temp. is displayed.

The high limit temp. can be changed by turning high limit regulator.


This is the end of set mode and the indicators display current temperature and CO

2

density.

Note:

● In each setting mode, if the change of the setting is not necessary, pressing set key (SET) skips to next setting mode.

● When the CO

2

density is set to 00.0, the control is OFF regardless of chamber density.

● The high limit temperature set value will change when the high limit regulator is turned even if the incubator is not in setting mode, because the alarm circuit is an independent circuit.

● In each set mode, the indicator returns to the current temperature and CO

2

density display mode automatically when 90 seconds has passed without any key operation.

26

SETTING OF ALARM RESUME TIME

The alarm buzzer is silenced by pressing the alarm buzzer stop key (BUZZER) on the control panel during alarm condition (The remote alarm is not canceled).

The buzzer will be activated again after certain suspension if the alarm condition is continued (The remote alarm continues). The suspension time can be set by following the procedure shown in the table below.

The example in the table is based on the assumption that the desired duration is 20 minutes.

Note: The duration is set in 30 minutes at the factory.

Table Changing procedure for alarm resume time (Ex: change from 30 minutes to 20 minutes)

Description of operation Key operated Indication after operation

1 ----

The current chamber temperature is displayed.

2

Press calibration key for 5 seconds.

CAL The left digit blinks.

3

Set the figure to F25 with the numerical value shift key and digit shift key. the figure of settable digit changes.

The settable digit is shifted.

4 Press enter key.

5

Set the figure to 020 with the numerical value shift key.

6 Press enter key.

ENT

ENT

The current setting is displayed.

The middle digit blinks.


The setting is memorized and the current chamber temperature is displayed.

• The settable alarm resume time are 0, 10, 20, 30, 40, 50, or 60 minutes (The setting is 000, 010, 020,

030, 040, 050 or 060 respectively). The buzzer would not reset if the resume time is set in 000.

• It is recommended to set the alarm resume time when the incubator is not under alarm condition. The setting during alarm condition is effective on the next alarm condition.

• The setting cannot be changed during power failure.

• The remote alarm during power failure cannot be stopped.

• The set mode returns to the temperature display mode automatically when 90 seconds has passed without any key operation. In this case, any setting before pressing enter key is not memorized.

Pressing calibration key (CAL) for about 5 seconds leads the calibration mode. In the calibration mode, the calibration of temperature and CO

2

density is possible. Wrong key operation affects the basic performance. Never touch any other keys on the control panel in the event of pressing calibration key

(CAL) accidentally. After about 90 seconds, the incubator returns to chamber temperature display mode automatically.

Operation after power failure

The set value is memorized by nonvolatile memory. Accordingly, the incubator resumes the operation with setting before power failure.

27

KEY LOCK FUNCTION

This incubator is provided with the key lock function. When the key lock is ON, change of temperature or

CO

2

density setting through the key pad is not available.

Note: The key lock is set in OFF mode at the factory.

Display Mode Function

1

Key lock is OFF Enable to change of temperature and CO

----

2

setting

Key lock is ON Disable to change of temperature or CO

Procedure for key lock setting (change from key lock OFF to key lock ON)

2

setting


The current chamber temperature and

CO

2 density are displayed.

2 Press digit shift key for 5 seconds.

3

Press numerical value shift key and scroll the figure to 1.

L0 is displayed in the digital temperature indicator.



The key lock is set to ON.

The current chamber temperature is displayed.

Note:

● The key lock function is available for temperature and CO

2

density setting.

● To cancel the key lock, set to L0 in the above procedure.

28

UV LAMP (MCO-18ACL with MCO-18UVS3)

The clauses below are applicable when an optional UV system set MCO-18UVS3 is installed.

The UV lamp is located inside the duct to sterilize the water in the humidifying pan and air circulating in the chamber.

 When all the inner attachments are installed correctly, only the inside of the duct and the inside of the humidifying pan cover are exposed to UV light.

 Correctly install all of the inner attachments when starting a cell culture, and never turn ON the UV lamp when the humidifying pan cover is removed.

 Always install the humidifying pan cover even when using the incubator without turning ON the UV lamp. Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity recovery performance.

 The UV lamp stays lit for a preset period of time after the outer door is closed. The default setting is for

5 minutes. The period can be changed when necessary as shown in the page 31.

 If the outer door is not opened for at least 12 consecutive hours, the UV lamp will light for the preset time period every 12 hours. The period can be changed when necessary as shown in the page 31.

 The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to approx.

70% of its initial value) is when the accumulated ON time reaches 1,000 hours. When the accumulated

ON time reaches approximately 1,000 hours, the UV indicator blinks (when the UV lamp is not lit). It is recommended that the UV lamp be quickly replaced at this point. Contact our sales representative or agent for information on replacing the UV lamp.

 If the UV lamp burns out, E18 is displayed on the digital temperature indicator. If this occurs, replace the UV lamp. When replacing the UV lamp, replace the glow starter (type FG-7P) at the same time.

Contact our sales representative or agent for information on replacing the UV lamp.

29


Precautions when using UV lamp


 Always use the humidifying pan and humidifying pan cover.

The humidifying pan and humidifying pan cover prevent UV light from escaping. Always use them even when not humidifying. To check whether the UV lamp is lit, open the outer door and then press the door switch with the inner door still closed. Visible blue light can be confirmed from the front of the humidifying pan cover. UV light is harmful to eyes, so do not light the UV lamp when the inner door or the humidifying pan cover is open.

WARNING

Never expose eyes directly to UV light as UV light can cause permanent damage to eyes. Never remove humidifying pan cover when UV light is ON (When an optional UV system set MCO-18UVS3 is installed in MCO-18ACL).

Hazardous UV light.

Do not press door switch when the inner door is opened..

 Be careful when handling the UV lamp.

There is a UV lamp inside of the duct. Be careful not to damage it when installing or removing the inner attachments or the humidifying pan.

30

Setting UV lamp ON period


Follow the procedure below when changing the setting of the UV lamp ON period.

Basic operation sequence (Example: change of UV lamp ON period from 5 minutes to 3 minutes)


1 Press calibration key for 5 seconds. CAL

The left digit in the digital temperature indicator is blinked.

2

By pressing numerical value shift key and digit shift key, set the figure to F01.

3 Press enter key. ENT the figure of settable digit changes.


The current setting is displayed in the digital CO

2 density indicator.

4

By pressing numerical value shift key, set the figure to 003.


Set value is memorized and the display return to

5 Press enter key. ENT normal display mode.

 The UV timer can be set from 0 minute to 30 minutes. The default setting is for 5 minutes. When the UV timer is set to zero, the UV lamp will not light.

 If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the door is closed, the lamp will light for the preset period of time.

 If the UV lamp ON time is set for longer than 5 minutes or if only the outer door is repeated opened and closed, condensation may occur in the chamber and may affect temperature distribution. It will also shorten the service life of the UV lamp.

 When replacing the UV lamp, contact our sales representative or agent.


2



31


Lighting UV lamp for 24 hours


If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to decontaminate the chamber by lighting the UV lamp for 24 hours.

1.

Remove all attachments from the chamber, including the trays, the tray supports, the side supports, the fan cover, the duct, the fan, the humidifying pan and the humidifying pan cover. Clean all the attachments in an autoclave or with alcohol for disinfection.

2.

Clean and wipe off inside the chamber with alcohol for disinfection.

3.

Set the CO

2 density to 0 % and set the UV 24-hour mode.


1 Press calibration key for 5 seconds. CAL

The left digit in the digital temperature indicator blinks.

2

By pressing numerical value shift key and digit shift key, set the figure to F02.


3 Press enter key. ENT the changeable digit is shifted.

“000” is displayed in the digital CO

2 density indicator.

4

By pressing numerical value shift key, set the figure to 001.

5 Press enter key. ENT the figure of settable digit changes.

Set value is memorized and the display returns to normal display mode.

· This procedure must be preformed only with the outer door closed and the UV lamp turned OFF.

· The UV lamp will light continuously for 24 hours after this parameter is set. The setting is canceled if the outer door is opened. Perform the above procedure to set the UV 24-hour mode again when opening the outer door.

· The incubator keeps running with set temperature and CO

2

density during UV lighting.

· Install the attachments after completion of the UV 24-hour mode.

Note: Set the high limit temperature alarm to at least 10 o C higher than the chamber set temperature when using the UV 24-hour mode of the UV lamp. The UV 24-hour mode may cause the automatic set temperature alarm because the temperature of the chamber will increase.


2



32

ALARMS, SAFETY, AND SELF-DIAGNOSIS

The incubator supports the following alarms, safety functions, and self-diagnostic functions.

Alarms, safety and self-diagnosis for culture operations

Alarm or safety function

High limit temperature alarm

If the chamber temperature exceeds the high limit temperature alarm set value.

Over heat lamp lights.

E12 or E16 and chamber temperature are displayed alternately.

Automatic set temperature alarm

Automatic set CO

2 density alarm

If the chamber temperature deviates from the set temperature by ±1 o C or more.

If the chamber CO

2

density deviates from the set value by ±1 % or more.

All digits on the digital temperature indicator blink.

All digits on the digital CO density indicator blink.

2

Continuous tone

Intermittent tone with

15 minutes delay.

Heater OFF

Remote alarm

Remote alarm with 15 minutes delay

Door check

Auto-return

Key lock

CO

2

gas cylinder empty

Chamber temperature sensor abnormality

Ambient temperature sensor abnormality

CO

2

sensor abnormality

Main heater abnormality

Bottom heater abnormality

Door heater abnormality

Disconnection of sensor for each heater

Low humidifying water*

UV lamp failure*

Recommendation of new UV lamp*

The outer door is open. Door lamp lights

When there is no key pressing in each setting mode for 90 seconds.

When the key lock is “ON”.

If the CO

2

density does not increase when the gas valve is opened.

If the temperature sensor is disconnected.

If the temperature sensor is short circuited.

If the ambient temperature sensor is disconnected.

If the ambient temperature sensor is short circuited.

Normal display mode.

-----

E01 is displayed alternately with the temperature on the digital temperature indicator.


CO

2

gas supply line indicator of empty blinks.





E11 is displayed alternately with the temperature on the digital temperature

If the output voltage of the CO

2 is abnormal.

sensor

If the high limit temperature alarm alarm operates, or if the main heater is open circuit, or the main heater relay is short circuited.

If the bottom heater goes open circuit, or the bottom heater relay is short circuited. indicator.



If the door heater goes open circuit, or the door heater relay is short circuited. indicator.


If the relay of main heater, bottom heater or sensor box heater goes open circuit.

If the water in the humidifying pan is about 0.8 L.

When the UV lamp is burned out.

The accumulated ON time is over about

1,000 hours. indicator.


Water level alarm lamp

(RH PAN) blinks.


UV indicator blinks when the UV lamp is OFF.

Intermittent tone with

15 minutes delay.

-----

-----

-----

Intermittent tone

Intermittent tone

Intermittent tone

Intermittent tone

Intermittent tone

Intermittent tone

Intermittent tone

Intermittent tone

Intermittent tone

Remote alarm with 15 minutes delay

Fan motor OFF

CO

2

valve is closed

UV lamp OFF*

(60 seconds after)

Heater OFF

The setting mode is canceled.

The setting is disabled.

Remote alarm

Gas supply line is altered.

Remote alarm

Heater OFF

Remote alarm

Remote alarm

CO

2

valve is closed

Remote alarm

Remote alarm

Remote alarm

Remote alarm

Remote alarm

----- -----

Intermittent tone Remote alarm

----- -----

*When an optional UV system set MCO-18UVS3 is installed.

• The alarm can be canceled by pressing the alarm buzzer stop key (BUZZER), but the remote alarm cannot be stopped. And the high limit temperature alarm cannot be silenced with the alarm buzzer stop key (BUZZER).

• E01 is cleared automatically when the gas is connected correctly and the buzzer is silenced with the alarm buzzer stop key (BUZZER). When MCO-21GC is installed, press the alarm buzzer stop key (BUZZER) to silence the alarm after changeover of gas supply line.

• If one of E05 to E18 (except for E12, E13, and E14) is displayed, contact our sales representative or agent.

33

CALIBRATION

Temperature calibration

Span setting

Span setting should be done under stable condition of temperature, humidity, and CO

2

density.

1.

Press the calibration key (CAL) once to enter the CO

2


2.

Press the calibration key (CAL) for approximately 5 seconds again to enter the temperature calibration input mode.

3.

The left digit of the digital temperature indicator blinks, and the digital CO

2

density indicator goes out.

4.

Set the present correct temperature with the digit shift key ( ) and numerical value shift key ( ) , then press the enter key (ENT).

5.

The incubator automatically reverts to the display mode.

[Example]

If the displayed chamber temperature is 37.0 o C (set value) and the actual temperature is 36.8 o C.

1.

Press the calibration key (CAL).

2.

Press the calibration key (CAL) for about 5 seconds.

3.

The “3” on the digital temperature indicator blinks, and the digital CO

2


4.

Adjust the set value to the actual value of 36.8 o C with the digit shift key ( ) and numerical value shift key ( ), then press enter key (ENT).

5.


Note:

It is important to accurately measure the temperature inside the incubator when calibrating the temperature. Particularly, the temperature gauge used must have an accuracy of JIS 0.5 Class or better.

The temperature must be measured at several points.

The temperature setting must not change by more than ±1.0 o C during calibration. If these values are exceeded, an error buzzer will sound, the input data will be ignored, and the incubator will return to the display mode. If it is necessary to change the temperature by more than 1.0 o C, perform calibration in several stages over a period of time.

34

CO

2

calibration

Span setting

Span setting should be done under stable condition of temperature, humidity, and CO

2

density.

1.

Press the calibration key (CAL) once to enter the CO

2


2.

Confirm blinking the decimal point in the digital CO

2

density indicator.

3.

Open the outer door and remove the sample air outlet cap on the inner door.

4.

Measure the CO

2

density in the chamber through the sample air outlet on the inner door.

5.

Press the calibration key (CAL) for about 5 seconds again to enter the temperature calibration input mode.

6.

The left digit on the digital temperature indicator blinks, and the digital CO

2


7.

Press the calibration key (CAL) once again to enter the CO

2

calibration input mode.

8.

The left digit on the digital CO

2

density indicator blinks, and the temperature indicator goes out.

9.

Set the present correct CO

2

density with the digit shift key ( ) and numerical value shift key ( ), then press the enter key (ENT).

10.

Replace the sample air outlet cap.

11.


Note:

This calibration is available when the setting of CO

2

density is 2 % or more.

[Example]

For an internal CO

2

density of 5.0 % (setting) and a measured value of 4.5 %.

1.

Press the calibration key (CAL) once.

2.

Press the calibration key (CAL) for about 5 seconds again.

3.

The “3” on the digital temperature indicator blinks, and the digital CO

2


4.

Press the calibration key (CAL) once again.

5.

The “0” on the digital CO

2

density indicator blinks, and the digital temperature indicator goes out.

6.

Set the present correct CO

2

density (4.5 %) with the digit shift key ( ) and numerical value shift key

( ), then press the enter key (ENT).

7.


35

TROUBLESHOOTING

If the incubator does not seem to be working properly, check the following items before calling for service.

Malfunction Check/Remedy

• The incubator is not plugged correctly into a power outlet.

The incubator does not operate at all.

• The circuit breaker at the power source is active or a power failure has occurred.

The key operation is disable

If the alarm function and the buzzer operates

If the chamber temperature is not equal to the set temperature

If the CO

2

gas density does not coincide with the set value

If the chamber humidity does not rise

If the CO

2 too much

consumption is

• The key lock function is set in ON mode.

[At the beginning of operation]

• The chamber temperature is not equal to the set value.

• The chamber CO

2

density is not equal to the set value. a. The secondary pressure of the gas regulator is not equal to the set value (0.03 MPa(G), 0.3 kgf/cm 2 (G), 4.4psi(G)). b. The gas tube is not connected securely between the gas regulator and the incubator.

[During operation]

• The high limit temperature alarm is not set at least 1 o C higher than the set chamber temperature.

• The set temperature value was changed, or the door was left open for a long period. Or a low temperature load was placed inside the incubator. In this case, if the incubator is left as it is, the alarm will eventually clear itself.

• The gas tube has slipped off or the gas leaks.

• The set value of the gas density was changed.

• The gas cylinder is empty. Check the primary pressure of the

CO

2

cylinder once a week. (The primary pressure of less than

3.8 MPa(G) (38 kgf/cm 2 (G), 551 psi(G)) means a little gas in the cylinder. Replace the cylinder soon.)

 It is used near an electric product which emits an electromagnetic wave.

• The ambient temperature must always be at least 5 o C less than the set temperature.

• The outer door was closed while the inner door was left open.


• The secondary pressure is not set to 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.4 psi(G)).

• The gas tube is clogged or chinked.

• The humidifying pan is not filled with sterile distilled water.

(Always use sterile distilled water.)


• The humidifying pan is not filled with sterile distilled water.

(Always use sterile distilled water.)

• The outer and inner doors are opened frequently.

• There is any gas leakage at the connection or pin hole on the gas tube. It is recommended to replace the gas tube once a year.

• The gasket of the inner door is not completely sealed.

• The access port at the upper left corner is opened.

If normal cultivation cannot be done and chamber gas density is suspect

• The environment around the incubator is not normal. The source of the contaminated gas is nearby.

• The incubator is installed in an enclosed space.

36

Malfunction Check/Remedy

If it takes much time to recover the CO

2

gas density

• HEPA filter is provided in the gas piping. If it takes much time to recover the gas density even though the gas pressure is normal, it may be that dust on the HEPA filter prevents the gas flow. Contact our sales representative or agent.

CO

2

gas is not being injected.

• The CO

2 control method for the gas density is the ON-OFF method. CO

2

gas is intermittently injected as the gas density in the chamber approaches the set value. Injections may be stopped for periods of approximately 15 seconds, but that is not an error.

 The gas is not injected until the temperature of the CO

2

sensor becomes stable enough approx. 1 hour, after turning ON the power switch or recovering from power failure.

Note: If the problem still has not been solved after trying the above checks and countermeasures, or for any problems not covered here, contact our sales representative or agent.

Keep an electric product which emits an electromagnetic wave away from this product. A noise from an electromagnetic wave may cause malfunction to this product.

DISPOSAL OF UNIT

When disposing of the CO

2

incubator, contact our sales representative or agent.

WARNING

The CO

2

incubator must be dismantled and disposed of by qualified personnel only . If the CO

2 incubator is left where outsiders enter, it may result unexpected accident (for example, children to become locked inside).

Before disposing a CO

2

incubator with biohazardous danger, decontaminate the CO

2

incubator to the extent possible by the user.

37

AUTOMATIC CO

2

CYLINDER CHANGEOVER

A gas auto changer (MCO-21GC) is available as an optional accessory. This system switches the gas supply line when one of the CO

2

gas cylinders is empty.

Note: The installation of the MCO-21GC must be performed by qualified service personnel only.

Install the MCO-21GC and then perform the following procedure

1.

Connect 2 gas tubes to connecting ports A and B for CO

2

gas pipes. Connecting ports A and B for CO

2 gas pipes are located on the left back of the incubator. (Refer to page 9).

Note: Only the port A is available when the MCO-21GC is not installed. Both ports of A and B are available when the MCO-21GC is installed.

2.

Connect a CO

2

gas cylinder equipped with a gas regulator to each gas tube. Refer to page 15 for information on connecting the CO

2

gas cylinders. (“Gas cylinder A” is the CO

2

gas cylinder connected to the connecting port A for CO

2

gas pipe, and “gas cylinder B” is the CO

2

gas cylinder connected to the connecting port B for CO

2

gas pipe.)

3.

Open the valve of both gas cylinders.

4.

The CO

2

gas supply line indicator of CO

2

gas supply line currently used is ON. (Only when MCO-21GC is installed.)

CO

2

gas supply line indicator A CO

2

gas supply line indicator B

CO

2

gas supply line switching key

CO

2

gas supply line indicator A

Current CO

2

gas supply line is A. ON

Current CO

2

gas supply line is B. OFF

5.

CO

2

gas supply line switches when one gas cylinder is empty.

The notice of change of gas supply line is as follows:

CO

2

gas supply line indicator B

OFF

ON

Digital temperature indicator CO

2

gas supply line indicator

When CO

2

gas supply line

A is switched to B

Current chamber temperature and E01 is displayed alternately.

CO

2

gas supply line indicator A blinks.

CO

2

gas supply line indicator B is ON.

automatically.

• By pressing the alarm buzzer stop key (BUZZER), the alarm is canceled and the incubator returns to the normal operation.

6. When the gas cylinder A is switched to the gas cylinder B, remove the CO

2

gas cylinder A and replace it with new one.

Note: Be careful when handling the empty CO

2

gas cylinder. Some gas may still be left in the cylinder.

• When the gas cylinder B becomes empty, the line is switched to the gas cylinder A again.

38

The MCO-21GC detects that there is no more CO

2

gas in a cylinder when the CO

2 density in the chamber does not increase for a while even after opening the CO

2

gas valve in the incubator. The supply line may also be switched for other reasons even though there is gas left in the cylinder: blocking or restricting of gas tube, reduction of CO

2

gas pressure, or improper opening of CO

2

gas cylinder. Always check the gas quantity in the cylinder before disconnecting it.

Following shows the procedure to change the CO

2

gas supply line manually. (Only when MCO-21GC is installed.)

(Example: Change of CO

2

gas cylinder from B to A)

1

2

3

(Gas cylinder B is currently used.)

Press CO

2

gas supply line switching key once.

(Pressing the key again selects gas cylinder B)

(Gas cylinder A is currently used.)

Operation

Key operated

Indication after operation

CO

2

gas supply line indicator

A B

CO

2

gas supply line indicator B is ON.

The CO

2

gas supply line indicator B changes OFF,

The CO

2

gas supply line indicator A changes ON.

CO

2

gas supply line indicator A is ON.

: indicator ON : indicator OFF

39

STACKING INCUBATORS

Use the following procedure to stack incubators. This work is potentially dangerous, so contact our sales representative or agent.

CAUTION

 Select a floor that is strong enough to support the stacked incubators.

 Never stack 3 or more incubators. Doing so is dangerous.

1. Take out 2 stacking plates A, 2 stacking plates B, 4 protective stickers, and 4 screws from the accessory bag.

Note:

When stacking 2 incubators, use 2 stacking plates (A and B) for each one . Only one pair of stacking plates is provided per an incubator, so take out the stacking plates (A and B) from both incubators.

Similarly only 2 protective stickers are provided per an incubator

2.

Unplug both of the incubators that are to be stacked.

3.

Remove the caps at the 2 places in the front of the top surface of the incubator that is to be on the bottom, and attach the protective stickers to the 4 corners on the top surface of the incubator that is to be on the bottom. (Fig. A)

4. Secure stacking plates A with the screws that are provided.

5. Remove the front panel (4 screws) from the incubator that is to be on the top.

6. Place the upper incubator on top of the lower one, being careful not to pinch your fingers.

7. Make the lower incubator level by adjusting the leveling feet, and then level the upper incubator in the same way.

8. Secure the stacking plates A on the top surface of lower incubator to the upper one with the accessory screws.

9. Remove from the back of the lower incubator the 2 hooks (4 screws) and remove from the upper incubator the 2 screws on the lower rear panel.

10. Use the 6 screws that were removed in step 9 to secure stacking plates B to the rear panels of the upper and lower incubators.

11. Replace the front panel (4 screws) to the upper incubator.

12. Use the hooks on the back of the upper incubator to prevent the incubators from falling over.

40

Front panel

Front side

Stacking plate A

Protective sticker

Fig. A

Stacking plate B

Hook

41

SPECIFICATIONS

Product name

External dimensions

Internal dimensions

Interior volume

CO

2

Incubator MCO-18ACL

W620 mm x D710 mm x H900 mm (W24.4 inch x D28.0 inch x H35.4 inch)


170 L (6.00 cu.ft.)

Interior

Outer door

Inner door

Trays

Access port

Insulation

Heating system

Stainless steel containing copper

Painted steel

Tempered glass

3 trays made of stainless steel containing copper


Maximum load: 7 kg/tray

Inner diameter: 30 mm (1.18 inch), On the back side

Rigid polyurethane foamed-in place

DHA system (heater jacket + air jacket system)

Humidifying system

Temperature controller

Temperature display

CO

2

controller

Natural evaporation with humidifying pan

PID control system

Digital display

ON-OFF control system/TC sensor

CO

2 density display

Air circulation

Air filter

Alarms

Digital display

Fan assisted

0.3 μ m, Efficiency; 99.97 % or more

Automatic set temperature alarm, Automatic set CO

2

density alarm

High limit temperature alarm, CO

2

gas, various sensor/heater alarms

(When a MCO-18UVS3 is installed) UV lamp failure alarm, Low humidifying water alarm

Allowable contact capacity: DC 30 V, 2 A

4 mm to 6 mm (0.157 inch to 0.236 inch) diameter tube can be connected

Remote alarm contacts

CO

2

inlet connection

CO

2

inlet pressure

Accessories

0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.4 psi(G))

3 trays, 3 sets of tray support, 1 gas tube, 1 humidifying pan,

1 pair of stacking plate A and B, 2 protective stickers, 2 tube bands

Weight

Optional accessories

92 k g

UV system set (MCO-18UVS3),

Interface board (MCO-420MA, USA only), Gas regulator (MCO-010R)

Tray (MCO-47ST) including 2 tray supports, Half tray (MCO-25ST)

Gas auto changer (MCO-21GC), Roller base (MCO-170RB)

Interface board (MTR-480)*, Interface board (MTR-L03)*

*Only for the Data acquisition system MTR-5000 user.

Note: Refer to the updated catalog when ordering an optional component.

Designs and specifications are subject to change without notice.

42

PERFORMANCE

Product name

Model number

Temperature control range

Temperature distribution

Temperature variation

CO

CO

2

2

control range

variation

Chamber humidity

Heat emission

Applicable environment condition

CO

2

Incubator MCO-18ACL

MCO-18ACL-PA

Ambient temperature +5 o C to 50 o C (ambient temperature; 5 o C to 35 o C)

Noise level

Power consumption

Rated voltage, frequency

Amperage

Note: It is based on our measuring method.

±0.25 o C (ambient temperature; 25 o C, setting; 37 o C, CO

2

5 %, no load)

±0.1 o C (ambient temperature; 25 o C, setting; 37 o C, CO

2

5 %, no load)

0 % to 20%

±0.15 % (ambient temperature; 25 o C, setting; 37 o C, CO

2

5 %, no load)

95 %R.H.±5 %R.H.

Max. 1116 kJ/h

Temperature: 5 o C to 35 o C, Humidity: equal or less than 80 %R.H.

(The designed performance may not be obtained when the ambient temperature is equal or less than 15 o C)

24 dB (A scale)

Max. 300 W

AC 110 V-120 V, 60 Hz

Max. 2.6 A

43

CAUTION

Please fill in this form before servicing.

Hand over this form to the service engineer to keep for his and your safety.

Safety check sheet

1. Incubator contents :

Risk of infection:

Risk of toxicity:

Risk from radioactive sources:

□

□

□

Yes

Yes

Yes

□

□

□

No

No

No

(List all potentially hazardous materials that have been stored in this unit.)

Notes :

2.

Contamination of the unit

Unit interior

No contamination

Decontaminated

□

□

Yes

Yes

□

□

No

No

□

Yes

□

No

Others:

3. Instructions for safe repair/maintenance/disposal of the unit a) The unit is safe to work on

□

Yes

□

No b) There is some danger (see below)

□

Yes

□

No

Procedure to be adhered to in order to reduce safety risk indicated in b) below.

Date :

Signature :

Address, Division :

Telephone :

Date of Installation : Product name :

CO

2

incubator

Model :

MCO-

Serial number :

Please decontaminate the unit yourself before calling the service engineer.

44

1-1-1 Sakada, Oizumi-machi, Ora-gun, Gunma 370-0596, Japan

© PHC Corporation 2018

Printed in Japan

LDCL039700-0

S0418-0

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