KERN & Sohn GmbH
Ziegelei 1 Tel: +49-[0]7433-9933-0
D-72336 Balingen, Germany
E-mail: [email protected]
Fax: +49 (0)7433-9933-149
Internet: www.kern-sohn.com
Operating manual
Analogue Brix Refractometer
KERN ORA 10 BB
ORA 18 BB
ORA 20 BB
ORA 32 BB
ORA 80 BB
Version 1.0
01/2015
GB
ORA_10_18_20_32_80BB-BA-e-1510
GB
KERN ORA
Version 1.0 01/2015
Operating instructions
Analogue refractometer
Contents
1
2
Technical data ................................................................................................ 3
Description ..................................................................................................... 4
3 General information ....................................................................................... 5
3.1
Intended use ............................................................................................................................. 5
3.2
Warranty .................................................................................................................................... 5
4 Basic safety information ............................................................................... 6
4.1
Follow the instructions in the operating manual .................................................................. 6
4.2
Warning ..................................................................................................................................... 6
8
9
5
6
Supplied items ............................................................................................... 7
Before the first use ........................................................................................ 7
7 Use/measurement .......................................................................................... 7
7.1
Zero point calibration ............................................................................................................... 8
7.2
Additional advice ...................................................................................................................... 9
7.3
Measuring procedure ............................................................................................................... 9
Cleaning and maintenance ........................................................................... 9
Storage ......................................................................................................... 10
10 Service .......................................................................................................... 10
11 Disposal ........................................................................................................ 10
12 Additional information ................................................................................. 10
13 Brix to refractive index (nD) conversion table .......................................... 11
14 Annex .............................................................................................................. 1
ORA_10_18_20_32_80BB-BA-e-1510 2
1 Technical data
Models
KERN
ORA 10BB
ORA 18BB
ORA 20BB
ORA 32BB
Measuring range and
Scales
0-10 % Brix
0-18 % Brix
0-20 % Brix
0-32 % Brix
Scale graduations
Accuracy
0.1% Brix
0.1% Brix
0.1% Brix
0.2 % Brix
ORA 80BB
0-80 % Brix 0.5 % Brix
Example scale on the ORA 18 BB
Dimensions
Product
189x40x40mm
189x40x40mm
189x40x40mm
178x40x40mm
137x40x40mm
Net weight
0.155kg
0.155kg
0.155kg
0.145kg
0.135 kg
3 ORA_10_18_20_32_80BA-e-1510
2 Description
3
4
2
5
1
6
1. Prism cover
2. Prism surface
3. Adjustment screw
4. Eyepiece with rubber eyeshade
5. Diopter adjustment ring
6. Optical tubes with rubber grip
9
8
10
11
7
7. Storage box
8. Pipette
9. Refractometer
10. Distilled water / calibration liquid
11. Adjustment tool
12. Cleaning cloth
ORA_10_18_20_32_80BB-BA-e-1510
12
4
3 General information
3.1 Intended use
The refractometer is a measuring instrument for determining the refractive index of transparent substances in liquid or in some cases also in the solid state. It is used to observe the behaviour of light as it passes from a prism with known properties to the substance being tested.
Use of the refractometer for other purposes is contrary to its intended use and may be hazardous. The manufacturer shall not be liable for any damages caused by improper use.
3.2 Warranty
The warranty shall be void in the event of:
Failure to observe the instructions in the operating manual
Use for purposes other than those described
Modifications or opening the device housing
Mechanical damage and/or damage resulting from media, liquids, natural wear and tear
5 ORA_10_18_20_32_80BA-e-1510
4 Basic safety information
4.1 Follow the instructions in the operating manual
Carefully read through the operating manual even if you have prior experience with KERN refractometers.
Every language version includes a non-authoritative translation.
The original German document is the definitive version.
4.2 Warning
Do not let acids come into contact with skin or eyes. If acid comes into contact with skin, flush with copious amounts of water. Shower if larger areas of skin are affected.
If acid comes into contact with eyes, keep the eyelid open and flush the eye with running lukewarm water from the outer corner to the inner corner. Flush eyes for at least 15 minutes. Then consult a doctor or ophthalmologist immediately.
Thoroughly clean the refractometer after each use.
The refractometer must not be exposed to extreme temperatures, high mechanical stresses, strong direct sunlight or high humidity.
This refractometer is not a toy. Keep out of reach of children.
Make sure that you will not be hit by anything else while you are using the refractometer, as this could cause serious eye injuries
The rubber eyeshade may cause irritation when in prolonged contact with the skin. If this happens, consult your doctor.
Do not touch the lenses with your fingers.
ORA_10_18_20_32_80BB-BA-e-1510 6
5 Supplied items
After unpacking and before using the device for the first time, check that all listed parts have been supplied. Replace damaged or faulty parts immediately and do not put them into operation.
Refractometer
Storage box
Pipette
Adjustment tool
Cleaning cloth
Distilled water / calibration liquid
6 Before the first use
Remove the protective film (if present) from the prism surface [2] and check that the rubber eye-cup [4] is fitted correctly.
7 Use/measurement
The refractometer can be used to quickly and accurately determine the refractive index of transparent substances, liquid or solid ones. To ensure correct measurement, the measuring device should be adjusted before measurements are carried out.
Please make sure your hands are dry before handling the measuring device.
7 ORA_10_18_20_32_80BA-e-1510
7.1 Zero point calibration
Hold up the refractometer against a sufficiently bright light source and look through the eyepiece [5], holding the rubber eyeshade [4] closely against your eye/glasses.
Rotate the eyepiece [5] to adjust it for your eyesight, until you can see the scale sharply.
Open the prism cover [1].
Thoroughly clean the prism [2] and the underside of the prism cover [1] using a soft cloth or soft paper (with alcohol if necessary) and wipe dry. Water should only ever be applied to the measuring prism, not to other parts of the device.
Now apply a few drops of the calibration liquid [10] onto the prism surface [2].
close the prism cover [1]. The amount of fluid should be sufficient to moisten most of the prism surface. There should not be any air bubbles between the measuring prism [2] and prism cover [1].
Wait about 30 seconds to allow the temperatures of the test fluid and prism to equalise.
Look through the eyepiece [4] while pointing the refractometer's prism surface
[2] at a bright light source.
Through the eyepiece [4], you will see a bright and a blue field. The boundary line between them shows the refractive index value on the scale which is also visible through the eyepiece [4].
Use the supplied adjustment tool [11] to turn the adjustment screw [3] behind the prism surface [2] (under the rubber cap), and adjust the scale so that the measuring device is optimally set up. The bright/dark boundary line should be at 0% Brix. For refractometers which are not adjusted at 0% Brix using distilled water and have a special calibration liquid, set the bright/dark boundary line to the calibration line in the field of view. The room temperature must be +20 °C.
For refractometers without automatic temperature compensation (ATC): Convert the measured value to take account of the deviation in room and sample temperature from +20 °C. (See the table at the end of this manual, Point 13.)
Repeat step 4 (cleaning).
Important!
The ambient/room temperature and the sample temperature influence the refractometer measuring result.
The scales are designed for an ambient temperature of +20 °C!
If the measurements are carried out at a temperature other than +20 °C, the results must be correspondingly corrected.
A correction table can be found in the annex, Point 13.
ORA_10_18_20_32_80BB-BA-e-1510 8
7.2 Additional advice
It is important that the samples being measured are representative samples. For samples that evaporate easily, measurements should be carried out quickly. The samples should be at the same temperature as the measuring instrument in order to achieve an accurate result.
7.3 Measuring procedure
Make sure your hands are dry before handling the refractometer.
Open the prism cover [1] and use the supplied pipette [8] to apply a few drops of the sample liquid [8] onto the prism surface [2], then close the prism cover
[1]. Spread the liquid evenly by pressing down on the prism cover [1] and eliminate any air bubbles present.
Hold the device horizontally and wait about 30 seconds (for optimal temperature equalisation between the sample and device).
View the measurement scale through the eyepiece [4]. Point the prism surface
[2] of the refractometer at a bright light source while doing this.
Rotate the adjustment ring [5] on the eyepiece [4] to adjust the focus.
The boundary line will move on the measurement scale depending on the concentration. This bright/dark boundary line shows the result on the scale.
If the temperature deviates from +20 °C, correct the measured result using the corresponding value from the temperature correction table [13].
Carefully clean the supplied pipette [8] and the refractometer after carrying out the measurement.
Important!
After every measurement, use an absorbent, lint-free cloth to remove the fluids from the prism surface [2] and prism cover [1]. Then carefully clean the prism and prism cover using a cloth moistened with water or if necessary alcohol, and dry both parts using a soft, dry and lint-free cloth. Avoid rubbing the prism [2].
8 Cleaning and maintenance
Clean the refractometer using a clean, lint-free cloth moistened with water or if necessary alcohol. Do not use any aggressive or abrasive cleaning agents. Never im-
9 ORA_10_18_20_32_80BA-e-1510
merse the device in water or hold it under running water. Never handle the device with wet or damp hands.
Never touch the measuring prism [2] with hard tools made from plastic, wood, rubber, metal, glass etc. Hard objects can quickly damage the relatively soft prism glass, resulting in measurement errors.
The refractometer is maintenance-free.
Cleaning should be carried out immediately before and after each use of the refractometer to maximise its life and optimise measurement results.
9 Storage
Store the refractometer in a dry, non-corrosive environment, preferably between
10°C and 30 °C.
10 Service
After reading this operating manual, if you have any questions about setting up or using the refractometer, or if any unexpected problem occurs, please contact your dealer. The device housing may only be opened by trained service technicians authorised by KERN.
11 Disposal
The packaging consists of environmentally friendly materials which can be disposed of via local recycling facilities.
The device and storage box should be disposed of by the operator in accordance with applicable national or regional regulations at the place of use.
12 Additional information
The product may differ slightly from the illustrations.
Avoid exposing the refractometer to direct sunlight.
Never bring the refractometer into contact with solvents.
ORA_10_18_20_32_80BB-BA-e-1510 10
13 Brix to refractive index (nD) conversion table
BRIX
Refractive index BRIX
Refractive index BRIX
Refractive index
% nD % nD % nD
1.35568
1.35729
1.35891
1.36054
1.36218
1.36384
1.36551
1.36720
1.36889
1.37060
1.37233
1.37406
1.37582
1.37758
1.33299
1.33442
1.33586
1.33732
1.33879
1.34026
1.34175
1.34325
1.34476
1.34629
1.34782
1.34937
1.35093
1.35250
1.35408
1.40978
1.41181
1.41385
1.41592
1.41799
1.42009
1.42220
1.42432
1.42647
1.42862
1.43080
1.43299
1.43520
1.43743
1.38115
1.38296
1.38478
1.38661
1.38846
1.39032
1.39220
1.39409
1.39600
1.39792
1.39986
1.40181
1.40378
1.40576
1.40776
53
54
55
56
57
58
49
50
51
52
45
46
47
48
41
42
43
44
37
38
39
40
30
31
32
33
34
35
36
23
24
25
26
27
28
19
20
21
22
15
16
17
18
11
12
13
14
7
8
9
10
3
4
5
6
0
1
2
29 1.37936 59 1.43967
Data from "ICUMSA" International Commission for Uniform Methods of Sugar Analysis, at 20 °C and 589 nm wavelength.
83
84
85
79
80
81
82
75
76
77
78
71
72
73
74
67
68
69
70
60
61
62
63
64
65
66
1.47787
1.48040
1.48295
1.48552
1.4881
1.49071
1.49333
1.49597
1.49862
1.50129
1.50398
1.44193
1.44420
1.44650
1.44881
1.45113
1.45348
1.45584
1.45822
1.46061
1.46303
1.46546
1.46792
1.47037
1.47285
1.47535
11 ORA_10_18_20_32_80BA-e-1510
14 Annex
Table 1: International Temperature Correction Table for °Brix (% sugar gradient)
Correct the result by the following values (refractometer must be correctly calibrated at 20 °C)
% Brix reading
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0
10.0
-0.53 -0.56 -0.59 -0.62 -0.65 -0.67 -0.69 -0.71 -0.72 -0.73 -0.74 -0.75 -0.75 -0.75 -0.75 -0.75 -0.74 -0.73
11.0
-0.49 -0.52 -0.54 -0.57 -0.59 -0.61 -0.63 -0.64 -0.65 -0.66 -0.67 -0.68 -0.68 -0.68 -0.68 -0.67 -0.67 -0.66
12.0
-0.44 -0.47 -0.49 -0.51 -0.53 -0.55 -0.56 -0.57 -0.58 -0.59 -0.60 -0.60 -0.61 -0.61 -0.60 -0.60 -0.60 -0.59
13.0
-0.40 -0.41 -0.43 -0.45 -0.47 -0.48 -0.50 -0.51 -0.52 -0.52 -0.53 -0.53 -0.53 -0.53 -0.53 -0.53 -0.52 -0.52
14.0
-0.34 -0.36 -0.38 -0.39 -0.40 -0.42 -0.43 -0.44 -0.44 -0.45 -0.45 -0.46 -0.46 -0.46 -0.46 -0.45 -0.45 -0.44
15.0
-0.29 -0.31 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37
16.0
-0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30
17.0
-0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22
18.0
-0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15
19.0
-0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07
20.0
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
21.0
0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07
22.0
0.13 0.14 0.14 0.14 0.15 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15
23.0
0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22
24.0
0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30
25.0
0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.39 0.38 0.37
26.0
0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.46
27.0
0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52
28.0
0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.64 0.63 0.62 0.61 0.60
29.0
0.66 0.67 0.68 0.69 0.70 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.68
30.0
0.74 0.75 0.77 0.78 0.79 0.80 0.81 0.81 0.81 0.82 0.81 0.81 0.81 0.80 0.79 0.78 0.77 0.75
ORA_10_18_20_32_80BB-BA-e-1510 1
