KERN Optics ORA 80 BE Operating Manual
KERN Optics ORA 80 BE is an analog refractometer designed for measuring the refractive index of transparent substances, like liquids and even some solids. This device is especially useful when working with sugar solutions and determining their Brix concentration. The ORA 80 BE features two distinct measurement ranges and a scale that allows for easy reading of results. It also has a built-in calibration function to ensure accuracy.
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3. General information GB 3.1 Intended use KERN & Sohn GmbH Ziegelei 1 D-72336 Balingen E-Mail: [email protected] Tel: +49-[0]7433- 9933-0 Fax: +49-[0]7433-9933149 Internet: www.kernsohn.com The refractometer is a measuring instrument for determining the refractive index of transparent substances in liquid or in some cases also in the solid state. It is used to observe the behaviour of light as it passes from a prism with known properties to the substance being tested. Use of the refractometer for other purposes is contrary to its intended use and may be hazardous. The manufacturer shall not be liable for any damages caused by improper use. Betriebsanleitung Analoges Refraktometer Operating manual Analogue refractometer KERN KERN ORA 80 BE Analog Expert 2-fach Skala Brix ORA 80 BE Analogue Expert 2x Brix scale Ziegelei 1 D-72336 Balingen E-Mail: [email protected] Version 1.1 KERN & Sohn GmbH 05/2016 Version 1.0 12/2014 Tel: +49- [0]7433-9933-0 Fax: +49-[0]7433-9933-149 Internet: www.kern-sohn.com ORA_80BE-BA-d-1410 The warranty shall be void in the event of: ▸▸ Failure to observe the instructions in the operating manual ▸▸ Use for purposes other than those described ▸▸ Modifications or opening the device housing ▸▸ Mechanical damage and/or damage resulting from media, liquids, natural wear and tear Contents 4. Basic safety information 1 2 3 3.1 3.2 4 4.1 4.2 5 6 7 7.1 7.2 7.3 8 9 10 11 12 13 14 4.1 Follow the instructions in the operating manual Technical data..................................................................................................1 Description......................................................................................................2 General information........................................................................................3 Intended use....................................................................................................3 Warranty..........................................................................................................3 Basic safety information.................................................................................4 Follow the instructions in the operating manual...........................................4 Warning........................................................................................................4-5 Supplied items.................................................................................................5 Before the first use..........................................................................................6 Use/measurement..........................................................................................6 Zero point calibration.................................................................................. 7-8 Additional advice.............................................................................................9 Measuring procedure................................................................................ 9-10 Cleaning and maintenance........................................................................... 11 Storage.......................................................................................................... 11 Service.......................................................................................................... 11 Disposal........................................................................................................ 12 Additional information................................................................................. 12 Brix to refractive index (nD) conversion table............................................. 13 Annex............................................................................................................ 14 ▸▸ Every language version includes a non-authoritative translation. The original German document is the definitive version. 4.2 Warning ▸▸ Do not let acids come into contact with skin or eyes. If acid comes into contact with skin, flush with copious amounts of water. Shower if larger areas of skin are affected. ▸▸ If acid comes into contact with eyes, keep the eyelid open and flush the eye with running lukewarm water from the outer corner to the inner corner. Flush eyes for at least 15 minutes. Then consult a doctor or ophthalmologist immediately. ▸▸ Thoroughly clean the refractometer after each use. ▸▸ The refractometer must not be exposed to extreme temperatures, high mechanical stresses, strong direct sunlight or high humidity. S kalen ORA 80BE ▸▸ Make sure that you will not be hit by anything else while you are using the refractometer, as this could cause serious eye injuries Net weight ▸▸ The rubber eyeshade may cause irritation when in prolonged contact with the skin. If this happens, consult your doctor. Genauig keit 180x40x40mm 0,400kg ORA Brix: 0 -­‐5 0 % 0 ,5 % Brix % Brix Brix: Brix: 5 0 -­‐ 00,5 ,5 % Brix 8 0 B E 0-50% 180x40x40mm 0,5 % Brix Brix: 50-80%8 0 % ▸▸ Do not touch the lenses with your fingers. 0,400kg Beispiel der Skala für den ORA 80 BE 5.Supplied items Example scale on ORA 80 BE After unpacking and before using the device for the first time, check that all listed parts have been supplied. Replace damaged or faulty parts immediately and do not put them into operation. 2 Gerätebeschreibung 6 6 2 3 4 1 2. Description 2 Gerätebeschreibung 3 6 3 6 Prismenabdeckung Prismenfläche Optische Röhre mit Griff Okular mit Einstellring des Diopters Messbereich Verstellrad Einstellschraube 1. 2. 3. 4. 5. 6. 9 Prismenabdeckung Prismenfläche 8 Röhre mit Griff Optische Okular mit Einstellring des Diopters Messbereich Verstellrad 10 Einstellschraube 4 5 11 9 7 12 8 7. Aufbewahrungsbox 8. Pipette ▸ Refractometer ▸ Storage box ▸ Pipette ▸ Adjustment tool ▸ Cleaning cloth ▸ Calibration liquid (Distilled water) 5 6. Before the first use 5 1 21. 2. 3. 4. 5. 6. 4 ▸▸ This refractometer is not a toy. Keep out of reach of children. Measuring Scale Dimensions 1 Technische Daten graduations Product range and Abmessungen Nettogewicht Mo d e lle Mes s bereic h S kalen-­‐ Scales Accuracy Produkt und t eilung K ERN Models KERN 3 ▸▸ Carefully read through the operating manual even if you have prior experience with KERN refractometers. 1.Technische Daten 1 3.2 Warranty 1. 2. 3. 4. 5. 6. Prism cover ORA_80BE-BA-d-1410 Prism surface Optical tubes with grip Eyepiece with diopter adjustment ring Adjusting wheel measuring range Adjusting screw Remove the protective film (if present) from the prism surface [2] and check that the rubber eye-cup [4] is fitted correctly. 7. Use/measurement The refractometer can be used to quickly and accurately determine the refractive index of transparent substances, liquid or solid ones. To ensure correct measurement, the measuring device should be adjusted before measurements are carried out. Please make sure your hands are dry before handling the measuring device. The measuring instrument has two measuring ranges. To switch between the two measuring ranges rotate the housing ring [5] between the eyepiece [4] and the optical tube [3]. 7. Storage box 8. Pipette 9. Refractometer 10. Calibration liquid (Distilled water) 11. Adjustment tool 12. Cleaning cloth 2 6 7.1 Zero point calibration 8. Cleaning and maintenance ▸▸ Hold up the refractometer against a sufficiently bright light source and look through the eyepiece [4], holding the eyepiece closely against your eye/glasses. ▸▸ Rotate the eyepiece [4] to adjust it for your eyesight, until you can see the scale sharply. ▸▸ Open the prism cover [1]. ▸▸ Thoroughly clean the prism [2] and the underside of the prism cover [1] using a soft cloth or soft paper (with alcohol if necessary) and wipe dry. Water should only ever be applied to the measuring prism, not to other parts of the device. ▸▸ Now apply a few drops of the calibration liquid [10] onto the prism surface [2]. ▸▸ close the prism cover [1]. The amount of fluid should be sufficient to moisten most of the prism surface. There should not be any air bubbles between the measuring prism [2] and prism cover [1]. ▸▸ Wait about 30 seconds to allow the temperatures of the test fluid and prism to equalise. ▸▸ Look through the eyepiece [4] while pointing the refractometer‘s prism surface [2] at a bright light source. ▸▸ Through the eyepiece [4], you will see a bright and a blue field. The boundary line 7 between them shows the refractive index value on the scale which is also visible through the eyepiece [4]. ▸▸ With the aid of the supplied adjustment tool [11] to turn the adjustment screw [3] at the side of the prism surface [2] the scale can be adjusted so that the measuring device is optimally set up by moving the boundary line upwards or downwards. Calibration value: 0 % (Brix) with water, or if necessary alcohol. Do not use any aggressive or abrasive cleaning agents. Never immerse the device in water or hold it under running water. Never handle the device with wet or damp hands. Never touch the measuring prism [2] with hard tools made from plastic, wood, glass, resulting in measurement errors. The refractometer is maintenance-free. refractometer to maximise its life and optimise measurement results. 9.Storage Store the refractometer in a dry, non-corrosive environment, preferably between 10 °C and 30 °C. 10.Service 11. Disposal The packaging consists of environmentally friendly materials which can be disposed of via local recycling facilities. The device and storage box should be disposed of by the operator in accordance with applicable national or regional regulations at the place of use. ▸▸ For refractometers without automatic temperature compensation (ATC): Convert the measured value to take account of the deviation in room and sample temperature from +20 °C. (See the table at the end of this manual, Point 14.) ▸▸ Repeat step 4 (cleaning). Important! 12. Additional information ! The ambient/room temperature and the sample temperature influence the refractometer measuring result. The scales are designed for an ambient temperature of +20 °C! If the measurements are carried out at a temperature other than +20 °C, the results must be correspondingly corrected. A correction table can be found in the annex, Point 14. The product may differ slightly from the illustrations. Avoid exposing the refractometer to direct sunlight. Never bring the refractometer into contact with solvents. 13. Brix to refractive index (nD) conversion table 1.37936 29 14. Annex Table 1: International Temperature Correction Table for °Brix (% sugar gradient) correctly ! After every measurement, use a lint-free, absorbent cloth to remove the fluids from the prism surface [2] and prism cover [1]. Then carefully clean the prism and prism cover using a cloth moistened with water and dry both parts using a soft, dry and lint-free cloth. Avoid rubbing the prism [2]. 10 Temperature °C 14 Annex Correct the result by the following values(%(refractometer must be Table 1: International Temperature Correction Table for °Brix sugar gradient) Correct the result by calibrated atthe following 20 °C). values (refractometer must be correctly calibrated at 20 °C) 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0 22.0 23.0 24.0 25.0 26.0 27.0 28.0 29.0 30.0 % Brix reading 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 -0.53 -0.56 -0.59 -0.62 -0.65 -0.67 -0.69 -0.71 -0.72 -0.73 -0.74 -0.75 -0.75 -0.75 -0.75 -0.75 -0.74 -0.73 -0.49 -0.52 -0.54 -0.57 -0.59 -0.61 -0.63 -0.64 -0.65 -0.66 -0.67 -0.68 -0.68 -0.68 -0.68 -0.67 -0.67 -0.66 -0.44 -0.47 -0.49 -0.51 -0.53 -0.55 -0.56 -0.57 -0.58 -0.59 -0.60 -0.60 -0.61 -0.61 -0.60 -0.60 -0.60 -0.59 -0.40 -0.41 -0.43 -0.45 -0.47 -0.48 -0.50 -0.51 -0.52 -0.52 -0.53 -0.53 -0.53 -0.53 -0.53 -0.53 -0.52 -0.52 -0.34 -0.36 -0.38 -0.39 -0.40 -0.42 -0.43 -0.44 -0.44 -0.45 -0.45 -0.46 -0.46 -0.46 -0.46 -0.45 -0.45 -0.44 -0.29 -0.31 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37 -0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30 -0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22 -0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07 0.13 0.14 0.14 0.14 0.15 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22 0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30 0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.39 0.38 0.37 0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.46 0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52 0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.64 0.63 0.62 0.61 0.60 0.66 0.67 0.68 0.69 0.70 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.68 0.74 0.75 0.77 0.78 0.79 0.80 0.81 0.81 0.81 0.82 0.81 0.81 0.81 0.80 0.79 0.78 0.77 0.75 ORA_80BE-BA-e-1510 14 Data from "ICUMSA" International Commission for Uniform Methods of Sugar Analysis, at 20 °C and 589 nm wavelength. 1.43743 1.43967 1.37758 28 59 1.43520 1.37582 27 58 1.37406 26 57 1.50129 1.50398 85 1.43299 1.43080 1.37233 25 56 1.49862 1.37060 24 55 84 1.42862 1.49597 1.36889 23 54 83 1.42647 1.36720 22 53 1.49333 81 82 1.42432 1.42220 1.36551 21 52 1.36384 20 51 1.4881 1.49071 80 1.42009 1.48552 1.36218 19 50 79 1.41799 1.48295 1.36054 18 49 78 1.41592 1.48040 1.35891 17 48 77 1.41385 1.47787 1.35729 16 47 76 1.41181 1.47535 1.35568 15 46 75 1.40978 1.47285 1.35408 14 45 74 1.40776 1.35250 13 44 1.46792 1.47037 72 71 73 1.40576 1.40378 1.40181 1.35093 12 43 1.34937 11 42 1.34782 10 41 1.46303 1.46546 70 1.39986 1.46061 1.34629 9 40 69 1.39792 1.34476 8 39 1.45822 67 68 1.39600 1.39409 1.34325 7 38 1.34175 6 37 1.45348 1.45584 66 1.39220 1.45113 1.34026 5 36 65 1.39032 1.44881 1.33879 4 35 64 1.38846 1.44650 1.33732 3 34 63 1.38661 1.33586 33 1.44420 61 62 1.38478 1.38296 1.33442 2 32 nD 1 31 60 1.38115 1.33299 0 30 Refractive index BRIX % nD Refractive index nD BRIX Refractive index 13 ▸▸ The boundary line will move on the measurement scale depending on the concentration. This bright/dark boundary line shows the result directly on the scale. ▸▸ If the temperature deviates from +20 °C, correct the measured result using the corresponding value from the temperature correction table [13]. ▸▸ Carefully clean the supplied pipette [8] and the refractometer after carrying out the measurement. % ▸▸ Make sure your hands are dry before handling the refractometer. ▸▸ Open the prism cover [1] and use the supplied pipette [8] to apply a few drops of the sample liquid [8] onto the prism surface [2], then close the prism cover [1]. Spread the liquid evenly by pressing down on the prism cover [1] and eliminate any air bubbles present. ▸▸ Hold the device horizontally and wait about 30 seconds (for optimal temperature equalisation between the sample and device). ▸▸ View the measurement scale through the eyepiece [4]. Point the prism surface [2] of the refractometer at a bright light source while doing this. 9 ▸▸ Rotate the adjustment ring [5] on the eyepiece [4] to adjust the focus. 12 BRIX 7.3 Measuring procedure 13 Brix to refractive index (nD) conversion table It is important that the samples being measured are representative samples. Measurements should be carried out quickly on samles that evaporate easily.If possible, the samples should be at the same temperature as the measuring instrument in order to achieve an accurate result. % 7.2 Additional advice 1.44193 Data from „ICUMSA“ International Commission for Uniform Methods of Sugar Analysis, at 20 °C and 589 nm wavelength. 8 Important! 11 After reading this operating manual, if you have any questions about setting up or using the refractometer, or if any unexpected problem occurs, please contact your dealer. The device housing may only be opened by trained service technicians authorised by KERN. ">
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Key features
Analogue refractometer
Brix scale
Two measurement ranges
Calibration function
Easy to read scale
Frequently asked questions
The ORA 80 BE is an analog refractometer used to measure the refractive index of transparent substances, primarily liquids, to determine their concentration, particularly in sugar solutions, using Brix units.
To calibrate the ORA 80 BE, you need to use the provided calibration liquid (usually distilled water) and follow these steps: clean the prism, apply a few drops of calibration liquid, close the prism cover, wait for temperature stabilization, and adjust the scale using the supplied tool until the boundary line aligns with the 0% Brix value.
After calibrating, clean the prism and apply a few drops of your sample. Close the cover, wait for temperature stabilization, and look through the eyepiece. The boundary line between the bright and blue field will indicate the Brix value on the scale.