To create a method for alternating ESI and APCI operation. Agilent Technologies G1978B

Methods 3

To create a method for alternating ESI and APCI operation

To create a method for alternating ESI and APCI operation

1 Open The MSD Spray Chamber dialog box by clicking Instrument > MSD

Spray Chamber in the Method and Run Control view.

2 Set Method Spray Chamber to MM-ES+APCI.

Figure 22 Method Spray Chamber set to MM-ES+APCI.

3 Check that Installed Spray Chamber is also set to MM-ES+APCI.

4 Make any other changes that are necessary for your method.

5 Click the OK button.

6 Open the Set up MSD Signals dialog box by clicking Instrument > Set Up

MSD Signals in the Method and Run Control view.

7 Modify the settings so that Signal 1 has Ionization value of MM-ES and

Signal 2 has Ionization value of MM-APCI as shown in Figure 23 .

8 Make any other changes that are necessary for your method.

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 39

3 Methods

To create a method for alternating ESI and APCI operation

N O T E

9 Click the OK button.

In general, use the mixed mode operation (MM-ES+APCI setting with Signal 1) instead of alternating MM-ES (Signal 1) and MM-APCI (Signal 2) mode switching. Two times as many scans are obtained during elution of a chromatographic peak, and no delay is needed between scans, resulting in better data. It is rarely necessary to know whether a compound responds purely in ESI or APCI modes on a chromatographic time scale.

Ionization mode for

Signal 1 and Signal 2

40

Figure 23 MM-ES and MM-APCI switching using Set Up MSD Signals dialog box

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide

Index

Index

A autotune,

32

C converting from ESI, APCI or APPI,

15

converting to ESI or APCI,

14

D diverter valve inlet filter,

20

E

ESI convert from,

15

convert to,

14

I installation,

7

, 21

L

LC/MSD sample tubing,

20

M method alternating ESI and APCI,

39

basic setup,

36

positive/negative mixed mode,

37

multimode nebulizer,

19

source image,

35

P parts multimode spray shield,

17

S spray shield for multimode source,

17

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 41

Index

42 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide

www.agilent.com

In This Book

This book contains installation, operation, maintenance and troubleshooting instruction for the Multimode Source for 6100 Series Single Quad

LC/MS.

©

Agilent Technologies, Inc. 2008

Printed in USA

First Edition, December 2008

*G1978-90070*

G1978-90070

Agilent Technologies